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湯本浩通

徳島大学

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2024年4月26日更新

職名: 教授
電話: 633-7343
電子メール: yumoto@tokushima-u.ac.jp
学歴: 1992/3: 徳島大学歯学部歯学科卒業
1996/3: 徳島大学大学院歯学研究科博士課程修了
学位: 博士(歯学) (徳島大学) (1996年3月)
職歴・経歴: 〜: 徳島大学助手, 大学院ヘルスバイオサイエンス研究部 (-2007.3.)
2007/4: 徳島大学助教, 大学院ヘルスバイオサイエンス研究部 (-2012.3.)
2012/4: 徳島大学講師, 病院 (-2017.9.)
2017/10: 徳島大学教授, 大学院医歯薬学研究部

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)

研究者総覧で最新データを確認する

2024年4月26日更新

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)
担当経験のある授業科目: 2022年度臨床能力試験説明用コンテンツ (学部)
SIH道場~アクティブ・ラーニング入門~(歯学部) (共通教育)
口腔と健康 (共通教育)
口腔保健推進予防歯学概論 (大学院)
口腔感染症学 (大学院)
実践口腔科学実習 (大学院)
歯内治療学 (学部)
歯周歯内治療学 (大学院)
歯周歯内治療学演習 (大学院)
歯周治療学各論 (学部)
歯周治療学実習 (学部)
歯周治療学総論 (学部)
歯周病学概論 (大学院)
歯科保存学(2) 実習 (学部)
歯科保存学(2)B 講義 (学部)
歯科保存学第二 (学部)
歯科薬理学 (学部)
研究基礎ゼミ (学部)
社会と歯科医療 (学部)
統合臨床講義 (学部)
総合歯科学二 (学部)
臨床実習(5年) (学部)
臨床実習(6年) (学部)
高齢者歯科学実験実習 (大学院)
指導経験: 2人 (博士)

研究者総覧で最新データを確認する

2024年4月26日更新

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)

研究テーマ: 口腔内細菌の付着に関する研究, 歯周病関連細菌と上皮細胞間の相互作用に関する研究, 歯周病関連細菌のバイオフィルム形成能に関する研究, 歯周病の心臓血管疾患への関わり, 歯髄炎の病態の解析(特に自然免疫機構の関連についての研究), 高周波治療の難治性根尖性歯周炎への応用

著書

湯本浩通 :
第3章ペリオドンタルメディシン歯周病と慢性腎臓病(CKD),
2023年2月. 湯本浩通 :
歯と歯根周囲の組織学,
永末書店, 2022年3月. Yuji Inagaki, Jun-ichi Kido, Yasufumi Nishikawa, Rie Kido, Eijiro Sakamoto, Mika Bandou, Koji Naruishi, Toshihiko Nagata and Hiromichi Yumoto :
Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis,
Jul. 2021. 二宮雅美, 湯本浩通 :
歯周病の病因,
株式会社永末書店, 2021年1月. 高柴正悟, 池田淳史, 湯本浩通, 二宮雅美, 生田貴久 :
WORKGROUP 2: PERIODONTITIS, 急性歯周疾患(歯周膿瘍と壊死性歯周疾患)と歯内―歯周病変,
2020年10月. Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Masami Ninomiya, Keiji Murakami, Hideki Fujii and Yoichiro Miyake :
The Pathogenic Factors from Oral Streptococci for Systemic Diseases,
Aug. 2020. 二宮雅美, 湯本浩通 :
特殊な歯周病の治療,
医歯薬出版株式会社, 2020年2月. 二宮雅美, 湯本浩通 :
クイズで学ぶ口腔疾患123, --- 歯・歯肉の異常 ---,
株式会社デンタルダイヤモンド社, 東京, 2019年3月.

(キーワード): 薬物性歯肉増殖症 / 壊死性潰瘍性歯周炎 / 侵襲性歯周炎 / 垂直性歯根破折

湯本浩通 :
歯根尖の炎症(根尖性歯周炎)むし歯科:高周波・電磁波治療の臨床試験,
2015年8月.

論文

Jun-ichi Kido, Yuka Hiroshima, Rie Kido, Kaya Yoshida, Yuji Inagaki, Koji Naruishi, Kazuaki Kajimoto, Masatoshi Kataoka, Yasuo Shinohara and Hiromichi Yumoto :
Lipocalin 2, synthesized using a cell-free protein synthesis system and encapsulated into liposomes, inhibits the adhesion of Porphyromonas gingivalis to human oral epithelial cells.,
Journal of Periodontal Research, Vol.58, No.2, 262-273, 2023.

(徳島大学機関リポジトリ): ● Metadata: 119199
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jre.13088
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36579753; ● Search Scopus @ Elsevier (PMID): 36579753; ● Search Scopus @ Elsevier (DOI): 10.1111/jre.13088

(徳島大学機関リポジトリ: 119199, DOI: 10.1111/jre.13088, PubMed: 36579753) Yasufumi Nishikawa, Yoritoki Tomotake, Hiromichi Kawano, Koji Naruishi, Jun-ichi Kido, Yuka Hiroshima, Akikazu Murakami, Tetsuo Ichikawa and Hiromichi Yumoto :
Effects of Candidalysin Derived from Candida albicans on the Expression of Pro-Inflammatory Mediators in Human Gingival Fibroblasts,
International Journal of Molecular Sciences, Vol.24, No.4, 3256, 2023.

(徳島大学機関リポジトリ): ● Metadata: 118834
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms24043256
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36834667; ● Search Scopus @ Elsevier (PMID): 36834667; ● Search Scopus @ Elsevier (DOI): 10.3390/ijms24043256

(徳島大学機関リポジトリ: 118834, DOI: 10.3390/ijms24043256, PubMed: 36834667) Yuka Hiroshima, Jun-ichi Kido, Rie Kido, Kaya Yoshida, Mika Bandou, Kazuaki Kajimoto, Hiromichi Yumoto and Yasuo Shinohara :
β-defensin 2 synthesized by a cell-free protein synthesis system and encapsulated in liposomes inhibits adhesion of Porphyromonas gingivalis to oral epithelial cells.,
Odontology, Vol.111, 830-838, 2023.

(徳島大学機関リポジトリ): ● Metadata: 119198
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10266-023-00789-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36745267; ● Search Scopus @ Elsevier (PMID): 36745267; ● Search Scopus @ Elsevier (DOI): 10.1007/s10266-023-00789-x

(徳島大学機関リポジトリ: 119198, DOI: 10.1007/s10266-023-00789-x, PubMed: 36745267) 川上歩花, 板東美香, 髙士友恵, 杉内美月, Mizusa Hyodo, 三島優奈, Masashi Kuroda, 森博康, 黒田暁生, 湯本浩通, 松久宗英, 阪上浩, 堤理恵 :
Umami taste sensitivity is associated with food intake and oral environment in subjects with diabetes,
The Journal of Medical Investigation : JMI, Vol.70, No.1.2, 241-250, 2023年.

(要約): Dysgeusia is a serious problem in patients with diabetes because it often leads to overeating, which is associated with disease progression. This study aimed to investigate the association between taste sensitivity, eating habits, and the oral environment. In this cross-sectional study of 75 subjects with diabetes, gustatory function was assessed using the whole-mouth method, and lingual taste receptor gene expression was measured by real-time PCR. Food intake was evaluated using a food frequency questionnaire based on food groups. The oral environment was assessed using xerostomia and periodontal comprehensive examination. In total, 45.3%, 28.0%, and 18.7% of subjects showed lower umami taste sensitivity, low sweet taste sensitivity, and low salt taste sensitivity, respectively. Lower umami sensitivity correlated with lower estimated glomerular filtration rate and higher energy-source food intake. Subjects with diabetes with higher plaque control record showed significantly higher T1R3 gene expression than those with lower plaque control record. Reduced umami taste sensitivity is associated with decreased renal function and high energy food intake in diabetes. Subjects with diabetes with higher plaque control record showed significantly higher T1R3 gene expression, suggesting that the oral environment affects taste gene expression. J. Med. Invest. 70 : 241-250, February, 2023.
(キーワード): Humans / Taste / Cross-Sectional Studies / Taste Perception / Diabetes Mellitus / Eating
(徳島大学機関リポジトリ): ● Metadata: 118208
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.70.241
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37164729; ● Summary page in Scopus @ Elsevier: 2-s2.0-85158866092

(徳島大学機関リポジトリ: 118208, DOI: 10.2152/jmi.70.241, PubMed: 37164729, Elsevier: Scopus) Yuta Uemura, Yuka Hiroshima, Ayano Tada, Keiji Murakami, Kaya Yoshida, Yuji Inagaki, Tomomi Kuwahara, Akikazu Murakami, Hideki Fujii and Hiromichi Yumoto :
Porphyromonas gingivalis Outer Membrane Vesicles Stimulate Gingival Epithelial Cells to Induce Pro-Inflammatory Cytokines via the MAPK and STING Pathways,
Biomedicines, Vol.10, No.10, 2643, 2022.

(要約): () is a keystone pathogen associated with chronic periodontitis and produces outer membrane vesicles (OMVs) that contain lipopolysaccharide (LPS), gingipains, and pathogen-derived DNA and RNA. -OMVs are involved in the pathogenesis of periodontitis. -OMV-activated pathways that induce the production of the pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in the human gingival epithelial cell line, OBA-9, were investigated. The role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in levels of -OMV-induced pro-inflammatory cytokines was investigated using Western blot analysis and specific pathway inhibitors. -OMVs induced IL-6 and IL-8 production via the extracellular signal-regulated kinase (Erk) 1/2, c-Jun N-terminal kinase (JNK), p38 MAPK, and NF-κB signaling pathways in OBA-9 cells. In addition, the stimulator of interferon genes (STING), an essential innate immune signaling molecule, was triggered by a cytosolic pathogen DNA. -OMV-induced IL-6 and IL-8 mRNA expression and production were significantly suppressed by STING-specific small interfering RNA. Taken together, these results demonstrated that -OMV-activated Erk1/2, JNK, p38 MAPK, STING, and NF-κB signaling pathways resulting in increased IL-6 and IL-8 expression in human gingival epithelial cells. These results suggest that -OMVs may play important roles in periodontitis exacerbation by stimulating various pathways.
(徳島大学機関リポジトリ): ● Metadata: 117616
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/biomedicines10102643
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36289904; ● Search Scopus @ Elsevier (PMID): 36289904; ● Search Scopus @ Elsevier (DOI): 10.3390/biomedicines10102643

(徳島大学機関リポジトリ: 117616, DOI: 10.3390/biomedicines10102643, PubMed: 36289904) Arya Adiningrat, Rifkhi Ade Kusnadi, Asyam Syafiq Allam, Erma Sofiani, Ikhsan Maulana and Hiromichi Yumoto :
The Effect of Probiotic Lactobacillus acidophilus and Ethanolic Propolis Compound toward Nucleic Acid Deposition in the Extracellular Polymeric Substance of Root Canal Bacteria,
European Journal of Dentistry, 2022.

(要約): This study aimed to evaluate the effects of ethanolic propolis and probiotic bacteria on the nucleic acid concentration in the extracellular polymeric substances (EPS) derived from biofilm of root canal bacteria. Clinical bacteria of the root canal were cultured with ethanolic extract of propolis (EEP; 10 or 0.1%) and . After the formation of biofilm was observed in the monolayer bacterial culture under several conditions, the enzymatic treatment and nucleic acid quantification were sequentially performed. Independent -test and Mann-Whitney were performed following data normality to analyze the significant differences of the treatment effect on the nucleic acid concentration in EPS from the isolated biofilm. The results showed that the nucleic acid concentration in EPS biofilm were not increased by coculture with as probiotics. However, the treatment with 10% EEP could significantly increase nucleic acid concentration. This study suggested that the biosurfactants from probiotic bacteria might be a promising candidate for endodontic treatment, arguably better than EEP in inhibiting biofilm maturation and complexity.
(徳島大学機関リポジトリ): ● Metadata: 118727
(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-0042-1750771
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35785820; ● Summary page in Scopus @ Elsevier: 2-s2.0-85134464904

(徳島大学機関リポジトリ: 118727, DOI: 10.1055/s-0042-1750771, PubMed: 35785820, Elsevier: Scopus) Yumi Kuwamura, Sumiko Yoshida, Kiyoe Kurahashi, Masuko Sumikawa, Hiromichi Yumoto, Hirokazu Uemura and Munehide Matsuhisa :
Effectiveness of a Diabetes Oral Nursing Program Including a Modified Diabetes Oral Health Assessment Tool for Nurses (M-DiOHAT©): A 12-Month Follow-Up Intervention Study,
The Journal of Medical Investigation : JMI, Vol.69, No.1,2, 86-96, 2022.

(要約): This study aimed to evaluate the effectiveness of a diabetes oral nursing intervention program for individuals with diabetes. Fifty-six participants with diabetes underwent a diabetes oral nursing intervention program. The program's effect was evaluated through questionnaires and small interviews. The modified diabetes oral health assessment tool (M-DiOHAT©) was used to assess and educate four factors;oral conditions, behaviors, perceptions and knowledge about diabetes and periodontal disease, and health information-sharing, among participants at baseline, 3, 6, and 12 months later. Primary outcomes included changes in the M-DiOHAT© total scores. Secondary outcomes included scores on the motivation stage of changes in oral health behaviors' scales, dental visits, number of present teeth, hemoglobin A1c (HbA1c), and participants' comments. The M-DiOHAT© total score and the motivation stage score significantly improved with the narrative comment of "being motivated to practice oral health behaviors" between the baseline and 12 months later. Eight participants visited the dentist, whereas no differences were observed in the number of present teeth or HbA1c. This program improved participants' M-DiOHAT© total score, motivation stage score, and dental visits. These results suggest the program improved oral health perceptions and behaviors among individuals with diabetes. J. Med. Invest. 69 : 86-96, February, 2022.
(キーワード): 糖尿病 (diabetes mellitus) / 看護介入 (nursing intervention) / 口腔保健行動 (oral health behaviors) / 認識
(徳島大学機関リポジトリ): ● Metadata: 117017
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.69.86
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35466152; ● Search Scopus @ Elsevier (PMID): 35466152; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.69.86

(徳島大学機関リポジトリ: 117017, DOI: 10.2152/jmi.69.86, PubMed: 35466152) Akihiro Wada, Keiji Murakami, Yumi Ishikawa, Takashi Amoh, Kouji Hirao, Yuki Hosokawa, Daisuke Hinode, Yoichiro Miyake and Hiromichi Yumoto :
Anti-Inflammatory and Protective Effects of Juncus effusus L. Water Extract on Oral Keratinocytes,
BioMed Research International, Vol.2022, 9770899, 2022.

(要約): Periodontitis is a chronic inflammatory disease caused by periodontopathogenic bacteria that form biofilms in periodontal pockets. The gingival epithelium acts as the first physical barrier in fighting attacks by periodontopathogenic pathogens, such as the primary etiological agent , and various exogenous chemicals, as well as regulates the local innate immune responses. Therefore, the development of novel oral care products to inhibit inflammatory reactions caused by bacterial infection and protect the gingival epithelium is necessary. L. has generally been used as an indigenous medicine, such as a diuretic, an antipyretic, and an analgesic, in ancient practice. In this study, we examined the effects of a water extract from L. on the inhibition of the inflammatory reaction elicited by bacterial infection and protection of the oral epithelium by chemical irritation. Pretreatment of oral epithelial cells with the water extract from L. significantly reduced or its lipopolysaccharide- (LPS-) mediated production of chemokines (interleukin-8 and C-C-chemokine ligand20) in a concentration-dependent manner with comparable to or greater effects than epigallocatechin gallate and protected oral epithelial cells from injury by chemical irritants, cetylpyridinium chloride, and benzethonium chloride. Moreover, the water extract from . L. in the presence of antimicrobial agents or antifibrinolytics already used as ingredients in mouthwash could significantly reduce the production of chemokines from LPS-stimulated oral epithelial cells in a concentration-dependent manner. These findings suggest that the water extract from . L. is potentially useful for oral care to prevent oral infections, such as periodontal infections, and maintain oral epithelial function.
(キーワード): Anti-Inflammatory Agents / Bacteroidaceae Infections / Cell Line, Transformed / Humans / Keratinocytes / Magnoliopsida / Mouth Mucosa / Periodontitis / Plant Extracts / Porphyromonas gingivalis
(徳島大学機関リポジトリ): ● Metadata: 117031
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2022/9770899
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35028318; ● Summary page in Scopus @ Elsevier: 2-s2.0-85123567270

(徳島大学機関リポジトリ: 117031, DOI: 10.1155/2022/9770899, PubMed: 35028318, Elsevier: Scopus) Rie Kido, Jun-ichi Kido, Yasufumi Nishikawa, Eijiro Sakamoto, Yoritoki Tomotake and Hiromichi Yumoto :
Diagnosis of inflammatory peri-implant diseases using an immunochromatographic assay for calprotectin in peri-implant crevicular fluid,
International Journal of Implant Dentistry, Vol.7, No.1, 106, 2021.

(要約): The incidence rate of peri-implant diseases is increasing with implant placement. Early detection of peri-implant diseases is important to prevent and treat these diseases, and a simple and objective diagnostic method is expected. Immunochromatographic (IC) assays are used for rapid diagnostic methods for some diseases. The aim of this clinical study was to determine the amount of calprotectin, an inflammatory marker, in peri-implant crevicular fluid (PICF) using an IC chip, and estimate the possibility of this diagnostic system. Forty-six individuals with dental implants participated in a pilot study. PICF samples were collected from the peri-implant sites with or without inflammation after clinical examinations including probing depth (PD), bleeding on probing (BOP) and gingival index (GI). Calprotectin in PICF was determined by an IC chip and enzyme-linked immunosorbent assay (ELISA) for calprotectin. The density of calprotectin line on the IC chip was measured using an IC reader (IC reader value). The relationship between IC reader value and ELISA value or clinical parameters was investigated. A receiver operating characteristic (ROC) curve analysis of IC reader value of calprotectin was performed to predict inflammation in peri-implant diseases. IC reader value of calprotectin was significantly correlated with its ELISA value and PD. IC reader values of calprotectin in PICF samples from periodontal sites with GI-1 and GI-2, and with BOP-positive sites were significantly higher than those of PICF samples from GI-0 sites, and BOP-negative sites, respectively. The IC reader value for calprotectin in PICF samples from inflammatory diseased sites was significantly higher than that of non-diseased sites. ROC analysis suggested that the IC reader value of PICF calprotectin was useful for predicting inflammatory peri-implant diseases. IC assay for PICF calprotectin may be a possible system for diagnosing the inflammatory peri-implant diseases.
(キーワード): Calprotectin / Diagnosis / Immunochromatographic assay / Peri-implant diseases / Peri-implant crevicular fluid
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s40729-021-00386-z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34623545; ● Search Scopus @ Elsevier (PMID): 34623545; ● Search Scopus @ Elsevier (DOI): 10.1186/s40729-021-00386-z

(DOI: 10.1186/s40729-021-00386-z, PubMed: 34623545) Wenhua Shao, Natsumi Fujiwara, Yasuhiro Mouri, Satoru Kisoda, Kayo Yoshida, Kaya Yoshida, Hiromichi Yumoto, Kazumi Ozaki, Naozumi Ishimaru and Yasusei Kudo :
Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells.,
Scientific Reports, Vol.11, No.1, 14943, 2021.

(要約): The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although Fusobacterium nucleatum mainly inhabits in oral cavity, attention has been focused on the F. nucleatum involvement in colorectal cancer development. Here we examined the p-EMT regulation by F. nucleatum in oral squamous cell carcinoma (OSCC) cells. We cultured OSCC cells with epithelial, p-EMT or EMT phenotype with live or heat-inactivated F. nucleatum. Expression of the genes involved in epithelial differentiation, p-EMT and EMT were examined in OSCC cells after co-culture with F. nucleatum by qPCR. Cell growth and invasion of OSCC cells were also examined. Both live and heat-inactivated F. nucleatum upregulated the expression of p-EMT-related genes in OSCC cells with epithelial phenotype, but not with p-EMT or EMT phenotype. Moreover, F. nucleatum promoted invasion of OSCC cells with epithelial phenotype. Co-culture with other strains of bacteria other than Porphyromonas gingivalis did not alter p-EMT-related genes in OSCC cells with epithelial phenotype. F. nucleatum infection may convert epithelial to p-EMT phenotype via altering gene expression in OSCC. Oral hygiene managements against F. nucleatum infection may contribute to reduce the risk for an increase in metastatic ability of OSCC.
(徳島大学機関リポジトリ): ● Metadata: 116445
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-021-94384-1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34294795; ● Summary page in Scopus @ Elsevier: 2-s2.0-85111135598

(徳島大学機関リポジトリ: 116445, DOI: 10.1038/s41598-021-94384-1, PubMed: 34294795, Elsevier: Scopus) Hiroo Tarao, Masatake Akutagawa, Takahiro Emoto, Amane Takei, Hiromichi Yumoto, Toshihiko Tominaga, Toshitaka Ikehara and Yosuke Kinouchi :
Evaluation of Temperature Increase from Joule Heat in Numerical Tooth Model by Applying 500 kHz Current for Apical Periodontitis Treatment Effect of applied voltage and tooth conductivity,
Bioelectromagnetics, Vol.42, No.3, 224-237, 2021.

(要約): For apical periodontitis treatments, a new method with the insertion of an electrode into the root canal of a tooth and application of a current at 500 kHz to sterilize the area by Joule heat has attracted attention. However, few studies have quantified the temperature increase in the root canal. This study aimed to investigate the basic characteristics of the temperature increase in a simple and standard tooth model when energizing a current at 500 kHz to the numerical tooth model with typical electrical and physical properties. We developed a numerical model of a standard tooth (dentin) and periodontal tissues consisting of an alveolar bone, cortical bone, and gingiva, and physiological saline in a root canal and calculated the temperature increase inside the numerical model by a coupled analysis of current and heat when a voltage was applied across the electrodes. The calculated results for the different applied voltages showed a temperature increase at the apical portion of the root canal, which increased with the applied voltage even for the same total supplied energy. The temperature increase occurred at the apical portion of the root canal as the tooth conductivity decreased. When the tooth conductivity was high, a current passed through the dentin, which led to a decrease in the temperature at the apical portion of the root canal. However, a chemical solution with a higher conductivity in the root canal tended to increase the temperature at the apical portion of the root canal, regardless of the tooth conductivity. More efficient approaches for increasing the spatial and temporal temperature for the tooth model target are needed. © 2021 Bioelectromagnetics Society.
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/bem.22325
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33537989; ● Search Scopus @ Elsevier (PMID): 33537989; ● Search Scopus @ Elsevier (DOI): 10.1002/bem.22325

(DOI: 10.1002/bem.22325, PubMed: 33537989) Yuji Inagaki, Jun-ichi Kido, Yasufumi Nishikawa, Rie Kido, Eijiro Sakamoto, Mika Bandou, Koji Naruishi, Toshihiko Nagata and Hiromichi Yumoto :
Gan-Lu-Yin (Kanroin), traditional Chinese herbal extracts, reduces osteoclast differentiation in vitro and prevents alveolar bone resorption in rat experimental periodontitis,
Journal of Clinical Medicine, Vol.10, No.3, 386, 2021.

(要約): Gan-Lu-Yin (GLY), a traditional Chinese herbal medicine, shows therapeutic effects on periodontitis, but that mechanism is not well known. This study aims to clarify the precise mechanism by investigating the inhibitory effects of GLY extracts on osteoclastogenesis in vitro and on bone resorption in periodontitis in vivo. RAW264.7 cells are cultured with soluble receptor activator of nuclear factor-kappa B (sRANKL) and GLY extracts (0.01-1.0 mg/mL), and stained for tartrate-resistant acid phosphatase (TRAP) to evaluate osteoclast differentiation. Experimental periodontitis is induced by placing a nylon ligature around the second maxillary molar in rats, and rats are administered GLY extracts (60 mg/kg) daily for 20 days. Their maxillae are collected on day 4 and 20, and the levels of alveolar bone resorption and osteoclast differentiation are estimated using micro-computed tomography (CT) and histological analysis, respectively. In RAW264.7 cells, GLY extracts significantly inhibit sRANKL-induced osteoclast differentiation at a concentration of more than 0.05 mg/mL. In experimental periodontitis, administering GLY extracts significantly decreases the number of TRAP-positive osteoclasts in the alveolar bone on day 4, and significantly inhibits the ligature-induced bone resorption on day 20. These results show that GLY extracts suppress bone resorption by inhibiting osteoclast differentiation in experimental periodontitis, suggesting that GLY extracts are potentially useful for oral care in periodontitis.
(徳島大学機関リポジトリ): ● Metadata: 116745
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/jcm10030386
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33498415; ● Search Scopus @ Elsevier (PMID): 33498415; ● Search Scopus @ Elsevier (DOI): 10.3390/jcm10030386

(徳島大学機関リポジトリ: 116745, DOI: 10.3390/jcm10030386, PubMed: 33498415) Tsuyoshi Kano, Tsuyoshi Morita, Kaori Sumida, Hiromichi Yumoto and Otto Baba :
Expression of fibroblast growth factor receptor1, -2c, and -3c transcripts in mouse molars after tooth eruption,
Anatomical Science International, 2021.

(キーワード): Dentin formation / FGF receptor / Odontoblasts / Secondary dentin / Tooth eruption
(徳島大学機関リポジトリ): ● Metadata: 116058
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12565-020-00594-4
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85099170531

(徳島大学機関リポジトリ: 116058, DOI: 10.1007/s12565-020-00594-4, Elsevier: Scopus) 西川泰史, 成石浩司, 木戸淳一, 湯本浩通 :
高グルコース条件下における歯肉線維芽細胞のカルプロテクチン誘導性炎症関連因子の産生におけるスダチチンの抑制効果,
日本歯科保存学雑誌, Vol.63, No.6, 503-511, 2020年.

(キーワード): 糖尿病 / 歯周病 / スダチチン / カルプロテクチン / 歯肉線維芽細胞 / diabetes mellitus / periodontitis / Sudachitin / calprotectin / gingival fibroblasts
(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.63.503
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390849931318242944; ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.63.503

(DOI: 10.11471/shikahozon.63.503, CiNii: 1390849931318242944) Koji Inagaki, Takeshi Kikuchi, Toshihide Noguchi, Akio Mitani, Keiko Naruse, Tatsuaki Matsubara, Masamitsu Kawanami, Jun Negishi, Yasushi Furuichi, Eiji Nemoto, Satoru Yamada, Hiromasa Yoshie, Koichi Tabeta, Sachiyo Tomita, Atsushi Saito, Sayaka Katagiri, Yuichi Izumi, Hiroshi Nitta, Takanori Iwata, Yukihiro Numabe, Matsuo Yamamoto, Nobuo Yoshinari, Tsuyoshi Fujita, Hidemi Kurihara, Fusanori Nishimura, Toshihiko Nagata, Hiromichi Yumoto, Toru Naito, Kazuyuki Noguchi, Koichi Ito, Shinya Murakami, Rimei Nishimura and Naoko Tajima :
A largescale observational study to investigate the current status of diabetic complications and their prevention in Japan (JDCP study 6): baseline dental and oral findings,
Diabetology International, Vol.12, No.1, 52-61, 2020.

(要約): Japan Diabetes Complication and Prevention prospective (JDCP) study was conducted to examine the association between glycemic control and oral conditions in a large database of Japanese patients with diabetes. It included a total of 6099 patients with diabetes (range, 40-75 years) who had been treated as outpatients between 2007 and 2009. The mean number of present teeth at baseline was 19.8 and women with type 2 diabetes had fewer teeth than men with type 2 diabetes. Within the previous year, 17% of all patients had lost teeth. At baseline, 32% had experienced gingival swelling, 69% had brushed more than twice a day, 37% had used interdental cleaning aids, and 43% had undergone regular dental checkups. Multiple logistic regression analysis indicated that type 1 patients with HbA1c ≥ 7.0% were at higher risk of having fewer than 20 teeth (odds ratio [OR] 2.38; 95% confidence interval [CI] 1.25-4.78), and type 2 patients with HbA1c ≥ 8.0% also were at high risk of having fewer than 20 teeth (OR 1.16; 95% CI 1.00-1.34), after adjustment for nine possible confounding factors. In conclusion, patients with diabetes were found to be at high risk of tooth loss, and the poorer the glycemic control, the higher the risk of tooth loss in these patients.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s13340-020-00465-3
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33479579; ● Search Scopus @ Elsevier (PMID): 33479579; ● Search Scopus @ Elsevier (DOI): 10.1007/s13340-020-00465-3

(DOI: 10.1007/s13340-020-00465-3, PubMed: 33479579) 桑村由美, 吉田守美子, 倉橋清衛, 澄川真珠子, 坂本英次郎, 黒田暁生, 粟飯原賢一, 船木真理, 湯本浩通, 上村浩一, 岡本美鈴, 大和光, 松久宗英, 遠藤逸朗, 岸田佐智 :
糖尿病患者の口腔保健行動アセスメントツールを用いた看護支援プログラムの評価,
糖尿病, Vol.63, No.Supplement, S117, 2020年.

(キーワード): 糖尿病 (diabetes mellitus) / 口腔保健行動 (oral health behaviors) / アセスメント (assessment) / 看護支援

Saori Matsui, Naomichi Yoneda, Hazuki Maezono, Katsutaka Kuremoto, Takuya Ishimoto, Takayoshi Nakano, Hiromichi Yumoto, Shigeyuki Ebisu, Yuichiro Noiri and Mikako Hayashi :
Assessment of the functional efficacy of root canal treatment with high frequency waves in rats,
PLoS ONE, Vol.15, No.9, e0239660, 2020.

(要約): The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-week-old male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, high-frequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-β1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis.
(キーワード): Animals / Bacteria / DNA, Bacterial / Dental Pulp Cavity / Fibroblast Growth Factor 2 / Imaging, Three-Dimensional / Interleukin-1beta / Male / Molar / Periapical Periodontitis / Radio Waves / Rats / Rats, Wistar / Root Canal Therapy / Transforming Growth Factor beta1 / X-Ray Microtomography
(徳島大学機関リポジトリ): ● Metadata: 116623
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0239660
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32991601; ● Search Scopus @ Elsevier (PMID): 32991601; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0239660

(徳島大学機関リポジトリ: 116623, DOI: 10.1371/journal.pone.0239660, PubMed: 32991601) Natsumi Fujiwara, Keiji Murakami, Kaya Yoshida, Shunsuke Sakurai, Yasusei Kudo, Kazumi Ozaki, Katsuhiko Hirota, Hideki Fujii, Maiko Suzuki, Yoichiro Miyake and Hiromichi Yumoto :
Suppresive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin.,
Heliyon, Vol.6, e04211, 2020.

(徳島大学機関リポジトリ): ● Metadata: 115916
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.heliyon.2020.e04211
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32577575; ● Summary page in Scopus @ Elsevier: 2-s2.0-85086425832

(徳島大学機関リポジトリ: 115916, DOI: 10.1016/j.heliyon.2020.e04211, PubMed: 32577575, Elsevier: Scopus) Yumi Kuwamura, Sumiko Yoshida, Kiyoe Kurahashi, Masuko Sumikawa, Eijiro Sakamoto, Ken-ichi Aihara, Hiromichi Yumoto, Akio Kuroda, Itsuro Endo, Toshiyuki Yasui and Sachi Kishida :
Modified diabetes oral health assessment tool (M-DiOHAT©) for nurses and their association with efficacy beliefs and outcome expectancies in patients with diabetes,
JNI : The Journal of Nursing Investigation, Vol.18, No.1, 13-26, 2020.

(キーワード): 糖尿病 (diabetes mellitus) / アセスメント (assessment) / 看護 (nursing) / 口腔保健行動 (oral health behaviors)
(徳島大学機関リポジトリ): ● Metadata: 114058
(出版サイトへのリンク): ● Publication site (DOI): 10.32273/jni.JNI_018_013
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.32273/jni.JNI_018_013

(徳島大学機関リポジトリ: 114058, DOI: 10.32273/jni.JNI_018_013) 稲垣幸司, 菊池毅, 野口俊英, 三谷章雄, 成瀬桂子, 川浪雅光, 根岸淳, 古市保志, 根本英二, 山田聡, 吉江弘正, 多部田康一, 富田幸代, 齊藤淳, 片桐さやか, 和泉雄一, 新田浩, 岩田隆紀, 沼部幸博, 山本松男, 吉成伸夫, 藤田剛, 栗原英見, 西村英紀, 永田俊彦, 湯本浩通, 内藤徹, 野口和行, 伊藤公一, 村上伸也, 西村理明, 田嶼尚子, 糖尿病データベース構築委員会 :
糖尿病合併症の実態とその抑制に関する大規模観察研究ベースライン時の口腔所見JDCP study 6,
糖尿病, Vol.63, No.4, 195-205, 2020年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11213/tonyobyo.63.195
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390566775131553664; ● Search Scopus @ Elsevier (DOI): 10.11213/tonyobyo.63.195

(DOI: 10.11213/tonyobyo.63.195, CiNii: 1390566775131553664) Rie Kido, Yuka Hiroshima, Jun-ichi Kido, Takahisa Ikuta, Eijiro Sakamoto, Yuji Inagaki, Koji Naruishi and Hiromichi Yumoto :
Advanced glycation end-products increase lipocalin 2 expression in human oral epithelial cells.,
Journal of Periodontal Research, Vol.55, No.4, 539-550, 2020.

(徳島大学機関リポジトリ): ● Metadata: 114681
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jre.12741
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32170733; ● Summary page in Scopus @ Elsevier: 2-s2.0-85081730917

(徳島大学機関リポジトリ: 114681, DOI: 10.1111/jre.12741, PubMed: 32170733, Elsevier: Scopus) 成石浩司, 坂本英次郎, 生田貴久, 木戸理恵, 木戸淳一, 湯本浩通 :
講義および実習試験の評価による歯周病学教育のあり方の考察,
日本歯科保存学雑誌, Vol.63, No.1, 22-29, 2020年.

(要約): 目的 : 一般的に, 臨床実習前の歯学部生に対する歯周病学の教育は, 座学講義と基礎 (模型) 実習を主体とする. 本研究の目的は, 歯周病学の講義および実習の評価試験の結果の関連性を調べ, 将来の歯学部生に対する歯周病学教育のあり方について考察することである. 方法 : 2018年度後期から2019年度前期に徳島大学歯学部に在籍し, 歯周病学を履修する歯学部3∼4年次生43名を対象とした. 歯周病学の講義前後に当該分野に関する筆記試験 (5肢2択形式) を行い, さらに歯周病学の基礎 (模型) 実習の終了時に, 顎模型を用いてスケーリング・ルートプレーニングの実習試験を行った. 講義による学生の理解度・習得度の評価は, カイ二乗検定を用いて判定した. 筆記試験および実習試験の結果の関連性は, スピアマンの順位相関検定を用いて判定した. また, 筆記試験の点数を25点満点で9点以下, 10∼12点, 13点以上の3群に分類し, 各群間の実習試験点数の差異の有無について, ANOVA-Tukey HSD解析を用いて判定した. なお, p値が0.05未満を有意差ありと判定した. 結果 : 歯周病学の講義後の筆記試験の点数は, 出題内容によって差はあるものの, 講義前と比較しておおむね有意に上昇した. 筆記試験の結果と実技試験の結果は, 有意に相関した (r=0.34, p=0.025). また筆記試験の点数が9点以下の群における実習試験の点数は, 筆記試験の点数が10∼12点, 13点以上の群と比較して有意に低かった (p<0.05). 一方, 筆記試験の点数が10∼12点と13点以上の群間における実習試験の点数に有意差は認められなかった (p=0.76). 結論 : 臨床実習前の歯周病学の学生教育において, 筆記試験の点数と基礎 (模型) 実習試験の点数は有意に相関した. このことは, 将来の効果的な歯周病学の教育システム構築の一助となる可能性を示唆する.
(キーワード): 歯学教育 / 歯周病学 / 基礎(模型)実習試験 / 筆記試験
(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.63.22
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390002184879112064; ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.63.22

(DOI: 10.11471/shikahozon.63.22, CiNii: 1390002184879112064) Ryosuke Takagi, Eijiro Sakamoto, Jun-ichi Kido, Yuji Inagaki, Yuka Hiroshima, Koji Naruishi and Hiromichi Yumoto :
S100A9 Increases IL-6 and RANKL Expressions through MAPKs and STAT3 Signaling Pathways in Osteocyte-Like Cells.,
BioMed Research International, Vol.2020, No.7149408, 2020.

(徳島大学機関リポジトリ): ● Metadata: 114641
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2020/7149408
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32149126; ● Search Scopus @ Elsevier (PMID): 32149126; ● Search Scopus @ Elsevier (DOI): 10.1155/2020/7149408

(徳島大学機関リポジトリ: 114641, DOI: 10.1155/2020/7149408, PubMed: 32149126) Natsumi Fujiwara, Hiromichi Yumoto, Koji Miyamoto, Katsuhiko Hirota, Hiromi Nakae, Saya Tanaka, Keiji Murakami, Yasusei Kudo, Kazumi Ozaki and Yoichiro Miyake :
2-Methacryloyloxyethyl phosphorylcholine (MPC)-polymer suppresses an increase of oral bacteria: a single-blind, crossover clinical trial,
Clinical Oral Investigations, Vol.23, 739-746, 2019.

(要約): The biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymers, which mimic a biomembrane, reduce protein adsorption and bacterial adhesion and inhibit cell attachment. The aim of this study is to clarify whether MPC-polymer can suppress the bacterial adherence in oral cavity by a crossover design. We also investigated the number of Fusobacterium nucleatum, which is the key bacterium forming dental plaque, in clinical samples. This study was a randomized, placebo-controlled, single-blind, crossover study, with two treatment periods separated by a 2-week washout period. We conducted clinical trial with 20 healthy subjects to evaluate the effect of 5% MPC-polymer mouthwash after 5 h on oral microflora. PBS was used as a control. The bacterial number in the gargling sample before and after intervention was counted by an electronic bacterial counter and a culture method. DNA amounts of total bacteria and F. nucleatum were examined by q-PCR. The numbers of total bacteria and oral streptcocci after 5 h of 5% MPC-polymer treatment significantly decreased, compared to the control group. Moreover, the DNA amounts of total bacteria and F. nucleatum significantly decreased by 5% MPC-polymer mouthwash. We suggest that MPC-polymer coating in the oral cavity may suppress the oral bacterial adherence. MPC-polymer can be a potent compound for the control of oral microflora to prevent oral infection.
(キーワード): 2-Methacryloyloxyethylphosphorylcholine / Clinical study / Oral infection / Oral bacteria / Fusobacterium nucleatum
(徳島大学機関リポジトリ): ● Metadata: 113011
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00784-018-2490-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29766378; ● Summary page in Scopus @ Elsevier: 2-s2.0-85046904798

(徳島大学機関リポジトリ: 113011, DOI: 10.1007/s00784-018-2490-2, PubMed: 29766378, Elsevier: Scopus) Hitomi Kuramoto, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Tadashi Nakanishi, Daisuke Takegawa, Ayako Washio, Chiaki Kitamura and Takashi Matsuo :
Caffeic acid phenethyl ester (CAPE) induces VEGF expression and production in rat odontoblastic cells,
BioMed Research International, Vol.Article ID 5390720, 2019.

(要約): Caffeic acid phenethyl ester (CAPE), the main component of propolis, has various biological activities including anti-inflammatory effect and wound healing promotion. Odontoblasts located in the outermost layer of dental pulp play crucial roles such as production of growth factors and formation of hard tissue termed reparative dentin in host defense against dental caries. In this study, we investigated the effects of CAPE on the upregulation of vascular endothelial growth factor (VEGF) and calcification activities of odontoblasts, leading to development of novel therapy for dental pulp inflammation caused by dental caries. CAPE significantly induced mRNA expression and production of VEGF in rat clonal odontoblast-like KN-3 cells cultured in normal medium or osteogenic induction medium. CAPE treatment enhanced nuclear factor-kappa B (NF-B) transcription factor activation, and furthermore, the specific inhibitor of NF-B significantly reduced VEGF production. The expression of VEGF receptor- (VEGFR-) 2, not VEGFR-1, was up regulated in KN-3 cells treated with CAPE. In addition, VEGF significantly increased mineralization activity in KN-3 cells. These findings suggest that CAPE might be useful as a novel biological material for the dental pulp conservative therapy.
(徳島大学機関リポジトリ): ● Metadata: 114566
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2019/5390720
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31930126; ● Summary page in Scopus @ Elsevier: 2-s2.0-85077441042

(徳島大学機関リポジトリ: 114566, DOI: 10.1155/2019/5390720, PubMed: 31930126, Elsevier: Scopus) Takuya Nii, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake :
Anti-inflammatory effects of olanexidine gluconate on oral epithelial cells,
BMC Oral Health, Vol.19, No.Article number 239, 2019.

(徳島大学機関リポジトリ): ● Metadata: 114367
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s12903-019-0932-0
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1186/s12903-019-0932-0

(徳島大学機関リポジトリ: 114367, DOI: 10.1186/s12903-019-0932-0) Kohei Nonaka, Mika Bandou, Eijiro Sakamoto, Yuji Inagaki, Koji Naruishi, Hiromichi Yumoto and Jun-ichi Kido :
6-Shogaol inhibits advanced glycation end-products-induced IL-6 and ICAM-1 expression by regulating oxidative responses in human gingival fibroblasts,
Molecules, Vol.24, No.20, e3705, 2019.

(要約): Advanced glycation end-products (AGEs) cause diabetes mellitus (DM) complications and accumulate more highly in periodontal tissues of patients with periodontitis and DM. AGEs aggravate periodontitis with DM by increasing the expression of inflammation-related factors in periodontal tissues. 6-Shogaol, a major compound in ginger, has anti-inflammatory and anti-oxidative activities. However, the influence of shogaol on DM-associated periodontitis is not well known. In this study, the effects of 6-shogaol on AGEs-induced oxidative and anti-oxidative responses, and IL-6 and ICAM-1 expression in human gingival fibroblasts (HGFs) were investigated. When HGFs were cultured with 6-shogaol and AGEs, the activities of reactive oxygen species (ROS) and antioxidant enzymes (heme oxygenase-1 [HO-1] and NAD(P)H quinone dehydrogenase 1 [NQO1]), and IL-6 and ICAM-1 expressions were investigated. RAGE expression and phosphorylation of MAPKs and NF-κB were examined by western blotting. 6-Shogaol significantly inhibited AGEs-induced ROS activity, and increased HO-1 and NQO1 levels compared with the AGEs-treated cells. The AGEs-stimulated expression levels of receptor of AGE (RAGE), IL-6 and ICAM-1 and the phosphorylation of p38, ERK and p65 were attenuated by 6-shogaol. These results suggested that 6-shogaol inhibits AGEs-induced inflammatory responses by regulating oxidative and anti-oxidative activities and may have protective effects on periodontitis with DM.
(徳島大学機関リポジトリ): ● Metadata: 114673
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/molecules24203705
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31619000; ● Summary page in Scopus @ Elsevier: 2-s2.0-85073474436

(徳島大学機関リポジトリ: 114673, DOI: 10.3390/molecules24203705, PubMed: 31619000, Elsevier: Scopus) Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Masami Ninomiya, Keiji Murakami, Hideki Fujii and Yoichiro Miyake :
The pathogenic factors from oral Streptococci for systemic diseases,
International Journal of Molecular Sciences, Vol.20, No.18, 2019.

(徳島大学機関リポジトリ): ● Metadata: 114574
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms20184571
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85072542634

(徳島大学機関リポジトリ: 114574, DOI: 10.3390/ijms20184571, Elsevier: Scopus) Keiko Aota, Kohichi Kani, Tomoko Yamanoi, Yukihiro Momota, Masami Ninomiya, Hiromichi Yumoto and Masayuki Azuma :
Management of tooth extraction in a patient with ELANE gene mutation-induced cyclic neutropenia: A case report.,
Medicine, Vol.98, No.39, e17372, 2019.

(要約): Cyclic neutropenia (CyN) is a rare hematological disease, and patients with CyN often experience an early onset of severe periodontitis and are forced to undergo tooth extraction. Here, we report a case of a patient with CyN who showed different periodicity and oscillations of neutrophil count compared with her mother, despite sharing the same novel genetic mutation. A 17-year-old Japanese girl who had been diagnosed with CyN shortly after birth presented to our hospital with a complaint of mobility of her teeth and gingivitis. Upon presentation, an intraoral examination was performed and revealed redness and swelling of the marginal and attached gingiva. Radiographs revealed extreme resorption of the alveolar bone and apical lesions in her mandibular lateral incisors. The patient's hematologic data demonstrated a lack of blood neutrophils (0/μL). The patient had no history of dental extraction, and her mother also had a history of CyN. The patient was diagnosed with severe periodontitis that was associated with CyN. Gene testing showed a novel heterozygous mutation in exon 4 of the ELANE gene (c.538delC, p.Leu180Ser fsX11). Based on the clinical findings, we planned to extract the patient's mandibular lateral incisors. Although the tooth extraction was scheduled considering the cyclic variation in neutrophil count, the patient's neutrophil count was 0/μL on the day before the planned extraction. Therefore, granulocyte-colony stimulating factor (G-CSF) was administered to increase the patient's neutrophil count. On the day of the patient's admission for the tooth extraction, she presented with fever (body temperature, 38.5°C), tonsillitis, and stomatitis. The extraction was subsequently delayed, and the patient was administered antibiotics and G-CSF for 4 days. At this time, the neutrophil count increased to 750/μL, and the tooth extraction was carried out safely. The postoperative course was uneventful, and the healing process at the extraction site was excellent. There is a possibility that the periodicity and oscillations of neutrophil count may change with growth in patients with CyN. Therefore, it is important to frequently examine and treat patients with fluctuating neutrophil levels for the management of invasive dental treatment in patients with CyN.
(キーワード): Adolescent / Exons / 女性 (female) / Humans / Leukocyte Count / Leukocyte Elastase / Mutation / Neutropenia / Neutrophils / 歯周病 (periodontitis) / Tooth Extraction
(徳島大学機関リポジトリ): ● Metadata: 115183
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/MD.0000000000017372
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31574885; ● Summary page in Scopus @ Elsevier: 2-s2.0-85072847226

(徳島大学機関リポジトリ: 115183, DOI: 10.1097/MD.0000000000017372, PubMed: 31574885, Elsevier: Scopus) Hiromichi Yumoto, Takashi Uebanso, Takaaki Shimohata and Akira Takahashi :
Current understanding of the gut microflora in subjects with nutrition-associated metabolic disorder such as obesity and/or diabetes: Is there any relevance with oral microflora?,
Current Oral Health Reports, Vol.6, No.2, 100-109, 2019.

(徳島大学機関リポジトリ): ● Metadata: 113630
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s40496-019-0221-7
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050566774698498304; ● Search Scopus @ Elsevier (DOI): 10.1007/s40496-019-0221-7

(徳島大学機関リポジトリ: 113630, DOI: 10.1007/s40496-019-0221-7, CiNii: 1050566774698498304) Eijiro Sakamoto, Jun-ichi Kido, Ryosuke Takagi, Yuji Inagaki, Koji Naruishi, Toshihiko Nagata and Hiromichi Yumoto :
Advanced glycation end-product 2 and Porphyromonas gingivalis lipopolysaccharide increase sclerostin expression in mouse osteocyte-like cells,
Bone, Vol.122, 22-30, 2019.

(要約): Sclerostin is a secreted glycoprotein that is mainly expressed in osteocytes, exerts negative effects on bone formation, and is present at elevated levels in diabetes mellitus (DM). Periodontitis is an infectious disease caused by periodontopathic bacteria, a complication of DM, and sometimes associated with severe inflammation and alveolar bone resorption. Advanced glycation end-products (AGEs) are a major pathogen in DM complications and adversely influence periodontitis in DM patients. In the present study, the effects of AGE2 and Porphyromonas gingivalis lipopolysaccharide (P-LPS) on the expression of sclerostin in mouse osteocyte-like cells (MLO-Y4-A2 cells) and its function in osteoblast differentiation were investigated. AGE2 and P-LPS up-regulated the expressions of receptor of AGE (RAGE) and Toll-like receptor 2 (TLR2), respectively, and significantly up-regulated that of sclerostin and interleukin 6 (IL-6) in osteocytes. Sclerostin, RAGE and TLR2 levels were synergistically increased by AGE2 and P-LPS. The siRNAs of RAGE and TLR2 significantly inhibited AGE2- and P-LPS-induced sclerostin expression. AGE2 up-regulated sclerostin expression in osteocyte-like cells via the RAGE, ERK and JNK, and NF-κB signal pathways. On the other hand, P-LPS elevated sclerostin levels via the TLR2, JNK and p38, and NF-κB signal pathways. When osteocytes pre-treated with AGE2 and P-LPS and osteoblastic cells (MC3T3-E1) were co-cultured in the medium with a sclerostin-neutralizing antibody, AGE2- and P-LPS-induced decreases in alkaline phosphatase activity and Runx2 expression in osteoblastic cells were significantly inhibited by the sclerostin-neutralizing antibody. These results suggest that AGE2 and P-LPS influence bone metabolism and inflammation through the regulation of sclerostin expression, and may aggravate periodontitis with DM.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bone.2019.02.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30735798; ● Search Scopus @ Elsevier (PMID): 30735798; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bone.2019.02.001

(DOI: 10.1016/j.bone.2019.02.001, PubMed: 30735798) Yoshihito Naitou, Hiromichi Yumoto, K Hs Kumar, Takashi Matsuo, Katsuhiko Hirota, Yoichiro Miyake, Kan Nagao, Yoritoki Tomotake, R Jimbo and Tetsuo Ichikawa :
Antifungal and Mechanical Properties of Tissue Conditioner Containing Plant-Derived Component, --- An In Vitro Study ---,
Journal of Prosthodontics, Vol.27, No.7, 665-669, 2018.

(要約): To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus-mixed tissue conditioner (dimensions: 10 mm in diameter and 2 and 6 mm in height for antimicrobial and mechanical tests, respectively) were prepared. The specimens placed on the bottom of the 24-well tissue culture plate were cultured with Candida albicans CAD1 for 2 and 4 days. The proliferation of the C. albicans in the wells was determined by measuring the optical density of fungal culture, and the surface of the specimens were also observed by scanning electron microscopy (SEM). To assess the mechanical properties of the specimens, the fluidity and hardness of Juncus-mixed tissue conditioner were measured using the methods certified according to ISO 10139-1. Juncus-mixed tissue conditioner significantly exhibited growth inhibitory effect in a Juncus concentration-dependent manner after both 2- and 4- day cultures. SEM observation showed that the amount of C. albicans on Juncus-mixed specimens drastically decreased, and biofilm formation was markedly inhibited. Moreover, both mechanical properties were found to be within the ranges regulated and specified by ISO. These findings demonstrated that the tissue conditioner including Juncus powder has a significant growth inhibitory effect against C. albicans, and it is suggested that the application of Juncus-mixed tissue conditioner may prevent denture stomatitis and oral candidiasis in denture wearers.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jopr.12546
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28901663; ● Search Scopus @ Elsevier (PMID): 28901663; ● Search Scopus @ Elsevier (DOI): 10.1111/jopr.12546

(DOI: 10.1111/jopr.12546, PubMed: 28901663) Koji Naruishi, Hiromichi Yumoto and Jun-ichi Kido :
Clinical effects of low body mass index on geriatric status in elderly patients.,
Experimental Gerontology, Vol.110, 86-91, 2018.

(要約): ). Significant associations of low BMI to several geriatric factors such as loss of posterior occlusion, cognitive impairment were observed in both male and female. FIM scores in above cut-off point group were significantly higher than in below cut-off point group in female (FIM gain, P = 0.0005; FIM efficiency, P = 0.0025, Mann-Whitney U test). On the other hand, there were no significant differences between low and above BMI cut-off point in FIM scores of male patients.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.exger.2018.05.017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29842897; ● Search Scopus @ Elsevier (PMID): 29842897; ● Search Scopus @ Elsevier (DOI): 10.1016/j.exger.2018.05.017

(DOI: 10.1016/j.exger.2018.05.017, PubMed: 29842897) Shinya Horiuchi, Hiromichi Yumoto, Tomoko Kimura, Minami Sato, Silvia Naomi Mitsui Akagi, Masahiro Hiasa, Eiji Nishikawa, Tarek El-Bialy and Eiji Tanaka :
Effect of pulsed ultrasound toothbrush on Streptococcus mutans biofilm removal.,
American Journal of Dentistry, Vol.31, No.2, 67-70, 2018.

(要約): The sonic vibration with pulsed ultrasound showed more reduction of the biofilm compared to the control and the sonic vibration with and without continuous ultrasound. Thus, pulsed ultrasound action may be beneficial for biofilm removal of interproximal regions.
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29630788; ● Summary page in Scopus @ Elsevier: 2-s2.0-85047330699

(PubMed: 29630788, Elsevier: Scopus) 平尾功治, 湯本浩通, 細川由樹, 蔵本瞳, 鷲尾絢子, 中西正, 武川大輔, 北村知昭, 松尾敬志 :
ラット象牙芽細胞様細胞 (KN-3) におけるカテキンの抗炎症作用,
日本歯科保存学雑誌, Vol.60, No.5, 235-244, 2017年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.60.235
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680498132224; ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.60.235

(DOI: 10.11471/shikahozon.60.235, CiNii: 1390282680498132224) Susilowati Heni, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Kouji Hirao, Katsuhiko Hirota, Takashi Matsuo and Yoichiro Miyake :
Royal jelly inhibits Pseudomonas aeruginosa adherence and reduces excessive inflammatory responses in human epithelial cells,
BioMed Research International, Vol.2017, 3191752, 2017.

(要約): is a Gram-negative bacterium and causes respiratory infection especially in elderly patients. Royal jelly has been used worldwide as a traditional remedy and as a nutrient; however, the effect against is unclear. The aim of this study was to analyze antibacterial, antiadherent, and anti-inflammatory effects of royal jelly against . Wild-type strain PAO1 and clinical isolates of were used for antibacterial assay and antiadherent assay to abiotic surface and epithelial cells, which are pharynx (Detroit 562) and lung (NCI-H292) epithelial cells. In anti-inflammatory assay, epithelial cells were pretreated with royal jelly before bacterial exposure to investigate its inhibitory effect on interleukin (IL-8) and macrophage inflammatory protein-3/CCL20 overproduction. Although royal jelly did not have antibacterial activity at concentration of 50% w/v, antiadherent activity was confirmed on the abiotic surface and epithelial cells under concentration of 25%. Pretreatment with royal jelly significantly inhibited overproduction of IL-8 and CCL20 from both cells. These results demonstrated that royal jelly inhibits adherence and protects epithelial cells from excessive inflammatory responses against infection. Our findings suggested that royal jelly may be a useful supplement as complementary and alternative medicine for preventing respiratory infection caused by .
(キーワード): Cell Line / Chemokine CCL20 / Epithelial Cells / Fatty Acids / Gene Expression Regulation / Humans / 炎症 (inflammation) / Interleukin-8 / Pseudomonas Infections / 緑膿菌 (Pseudomonas aeruginosa)
(徳島大学機関リポジトリ): ● Metadata: 112385
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2017/3191752
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29075644; ● Summary page in Scopus @ Elsevier: 2-s2.0-85030761989

(徳島大学機関リポジトリ: 112385, DOI: 10.1155/2017/3191752, PubMed: 29075644, Elsevier: Scopus) Sekita Yasuko, Keiji Murakami, Hiromichi Yumoto, Kouji Hirao, Takashi Amoh, Natsumi Fujiwara, Katsuhiko Hirota, Hideki Fujii, Takashi Matsuo, Yoichiro Miyake and Yoshiki Kashiwada :
Antibiofilm and Anti-Inflammatory Activities of+ Houttuynia cordata Decoction for Oral Care,
Evidence-Based Complementary and Alternative Medicine : eCAM, Vol.2017, 2850947, 2017.

(要約): Dental biofilms that form in the oral cavity play a critical role in the pathogenesis of several infectious oral diseases, including dental caries, periodontal disease, and oral candidiasis. (HC, Saururaceae) is a widely used traditional medicine, for both internal and external application. A decoction of dried HC leaves (dHC) has long been consumed as a health-promoting herbal tea in Japan. We have recently reported that a water solution of HC poultice ethanol extract (wHCP) exerts antimicrobial and antibiofilm effects against several important oral pathogens. It also exhibits anti-inflammatory effects on human keratinocytes. In our current study, we examined the effects of dHC on infectious oral pathogens and inflammation. Our results demonstrated that dHC exerts moderate antimicrobial effects against methicillin-resistant (MRSA) and other oral microorganisms. dHC also exhibited antibiofilm effects against MRSA, (involved in dental plaque formation), and and inhibitory effects on interleukin-8, CCL20, IP-10, and GRO productions by human oral keratinocytes stimulated by lipopolysaccharide (a cause of periodontal disease), without cytotoxic effects. This suggests that dHC exhibits multiple activities in microorganisms and host cells. dHC can be easily prepared and may be effective in preventing infectious oral diseases.
(徳島大学機関リポジトリ): ● Metadata: 112956
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2017/2850947
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29234378; ● Summary page in Scopus @ Elsevier: 2-s2.0-85042323475

(徳島大学機関リポジトリ: 112956, DOI: 10.1155/2017/2850947, PubMed: 29234378, Elsevier: Scopus) Natsumi Fujiwara, Keiji Murakami, Michiyasu Nakao, Munehisa Toguchi, Hiromichi Yumoto, Katsuhiko Hirota, Takashi Matsuo, Shigeki Sano, Kazumi Ozaki and Yoichiro Miyake :
Antibacterial and antibiofilm effects of Reuterin-Related Compounds to periodontopathic bacteria,
Journal of Oral Health and Biosciences, Vol.30, No.1, 8-17, 2017.

(徳島大学機関リポジトリ): ● Metadata: 111063
(出版サイトへのリンク): ● Publication site (DOI): 10.20738/johb.30.1_8
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.20738/johb.30.1_8

(徳島大学機関リポジトリ: 111063, DOI: 10.20738/johb.30.1_8) Natsumi Fujiwara, Keiji Murakami, Michiyasu Nakao, Munehisa Toguchi, Hiromichi Yumoto, Takashi Amoh, Katsuhiko Hirota, Takashi Matsuo, Shigeki Sano, Kazumi Ozaki and Yoichiro Miyake :
Novel reuterin-related compounds suppress odour by periodontopathic bacteria,
Oral Diseases, Vol.23, No.4, 492-497, 2017.

(要約): Halitosis is caused by volatile sulphur compounds including methyl mercaptan (CH3 SH) in the oral cavity and is a serious problem that limits interpersonal social communication. The aim of study was to evaluate the effects of reuterin-related compounds (RRCs) on halitosis-related periodontopathic bacteria in vitro. RRC-01, RRC-02 and RRC-03 (32 and 64 g ml(-1) ) in culture media containing Fusobacterium nucleatum JCM8523 and Porphyromonas gingivalis ATCC33277 were used. The effects of RRCs on CH3 SH production and detectable odour by F. nucleatum and P. gingivalis were examined by CH3 SH production assay and organoleptic test, respectively. The number of bacterial cells was also measured using an ATP assay. In P. gingivalis treated with RRCs, the expression of mgl gene, which is responsible for CH3 SH production, was examined by qRT-PCR. CH3 SH production and the score of detectable odour from F. nucleatum and P. gingivalis culture media containing RRCs were significantly lower than that without RRCs (P < 0.05). The expression of mgl gene in P. gingivalis was significantly downregulated by RRC-01 (P < 0.01), but not by RRC-02 or RRC-03. RRCs are potent oral care products for preventing halitosis via reducing CH3 SH production.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/odi.12638
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28083982; ● Summary page in Scopus @ Elsevier: 2-s2.0-85015084211

(DOI: 10.1111/odi.12638, PubMed: 28083982, Elsevier: Scopus) Keiji Murakami, Hiromichi Yumoto, Ayu Murakami, Takashi Amoh, Darija Viducic, Katsuhiko Hirota, Atsushi Tabata, Hideaki Nagamune, Hiroki Kourai, Takashi Matsuo and Yoichiro Miyake :
Evaluation of the effectiveness of the potent bis-quaternary ammonium compound, 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) on Pseudomonas aeruginosa.,
Journal of Applied Microbiology, Vol.122, No.4, 893-899, 2017.

(要約): Quaternary ammonium compounds (QACs), including benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC) are cationic surfactants and have been used widely as general disinfectants in the medical field due to their strong antibacterial effects and low cytotoxicity to human cells. 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) is one of the potent bis-QACs synthesized to improve the antimicrobial activities of mono-QACs such as BAC. This study aimed to assess the effectiveness of 4DTBP-6,8 against Pseudomonas aeruginosa, a prevalent hospital pathogen. The minimum inhibitory concentrations of 4DTBP-6,8, CPC and BAC against P. aeruginosa were measured. 4DTBP-6,8 exhibited strong antibacterial activity. We assessed the bactericidal effects of QACs against P. aeruginosa under certain conditions and their cytotoxicities in human epithelial cells using lactate dehydrogenase (LDH) release. 4DTBP-6,8 exerted excellent bactericidal effects against high concentrations of bacteria, biofilm cells and even in the presence of contaminated proteins. Cellular LDH was not released by the treatment with 4DTBP-6,8. 4DTBP-6,8 exhibited the strongest bactericidal activity against P. aeruginosa among the three QACs tested without any cytotoxicity. The potent bis-QAC, 4DTBP-6,8 has the potential to be an effective disinfectant in preventing hospital infections caused by P. aeruginosa.
(キーワード): Anti-Bacterial Agents / Biofilms / Cell Line / Disinfectants / Humans / Niacinamide / 緑膿菌 (Pseudomonas aeruginosa) / Pyridinium Compounds
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.13392
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28035713; ● Summary page in Scopus @ Elsevier: 2-s2.0-85013236835

(DOI: 10.1111/jam.13392, PubMed: 28035713, Elsevier: Scopus) Katsuhiko Hirota, Hiromichi Yumoto, B Sapaar, Takashi Matsuo, Tetsuo Ichikawa and Yoichiro Miyake :
Pathogenic factors in Candida biofilm-related infectious diseases.,
Journal of Applied Microbiology, Vol.122, No.2, 321-330, 2016.

(要約): Candida albicans is a commonly found member of the human microflora and is a major human opportunistic fungal pathogen. A perturbation of the microbiome can lead to infectious diseases caused by various micro-organisms, including C. albicans. Moreover, the interactions between C. albicans and bacteria are considered to play critical roles in human health. The major biological feature of C. albicans, which impacts human health, resides in its ability to form biofilms. In particular, the extracellular matrix (ECM) of Candida biofilm plays a multifaceted role and therefore may be considered as a highly attractive target to combat biofilm-related infectious diseases. In addition, extracellular DNA (eDNA) also plays a crucial role in Candida biofilm formation and its structural integrity and induces the morphological transition from yeast to the hyphal growth form during C. albicans biofilm development. This review focuses on pathogenic factors such as eDNA in Candida biofilm formation and its ECM production and provides meaningful information for future studies to develop a novel strategy to battle infectious diseases elicited by Candida-formed biofilm.
(キーワード): Biofilms / Candida / Candida albicans / Candidiasis / DNA (DNA) / Humans / Hyphae / クォーラムセンシング (quorum sensing) / Respiratory Tract Infections / Virulence Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.13330
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27770500; ● Summary page in Scopus @ Elsevier: 2-s2.0-85005943779

(DOI: 10.1111/jam.13330, PubMed: 27770500, Elsevier: Scopus) Yuki Hosokawa, Kouji Hirao, Hiromichi Yumoto, Ayako Washio, Tadashi Nakanishi, Daisuke Takegawa, Chiaki Kitamura and Takashi Matsuo :
Functional roles of NOD1 in odontoblasts on dental pulp innate immunity,
BioMed Research International, Vol.2016, 9325436, 2016.

(要約): Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.
(キーワード): Animals / Cell Line / 細胞質分裂 (cytokinesis) / Dental Pulp / 自然免疫 (innate immunity) / Inflammation Mediators / Nod1 Signaling Adaptor Protein / Odontoblasts / Rats
(徳島大学機関リポジトリ): ● Metadata: 112386
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2016/9325436
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27747243; ● Summary page in Scopus @ Elsevier: 2-s2.0-84991669747

(徳島大学機関リポジトリ: 112386, DOI: 10.1155/2016/9325436, PubMed: 27747243, Elsevier: Scopus) Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Natsumi Fujiwara, Shohei Ogata, Takashi Matsuo, Yoichiro Miyake and Yoshiki Kashiwada :
Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases.,
BioMed Research International, Vol.2016, 2581876, 2016.

(要約): Houttuynia cordata (HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey showed that HC poultice (HCP) prepared from smothering fresh leaves of HC had been frequently used for the treatment of purulent skin diseases with high effectiveness. Our experimental study also demonstrated that ethanol extract of HCP (eHCP) has antibacterial, antibiofilm, and anti-inflammatory effects against S. aureus which caused purulent skin diseases. In this study, we focused on novel effects of HCP against oral infectious diseases, such as periodontal disease and dental caries. We determined the antimicrobial and antibiofilm effects of water solution of HCP ethanol extract (wHCP) against important oral pathogens and investigated its cytotoxicity and anti-inflammatory effects on human oral epithelial cells. wHCP had moderate antimicrobial effects against some oral microorganisms and profound antibiofilm effects against Fusobacterium nucleatum, Streptococcus mutans, and Candida albicans. In addition, wHCP had no cytotoxic effects and could inhibit interleukin-8 and CCL20 productions by Porphyromonas gingivalis lipopolysaccharide-stimulated human oral keratinocytes. Our findings suggested that wHCP may be clinically useful for preventing oral infectious diseases as a mouthwash for oral care.
(キーワード): Anti-Bacterial Agents / 細菌 (bacteria) / Biofilms / Cell Line / Cell Survival / Dental Caries / Houttuynia / Humans / Periodontal Diseases / Plant Extracts
(徳島大学機関リポジトリ): ● Metadata: 112384
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2016/2581876
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27413739; ● Summary page in Scopus @ Elsevier: 2-s2.0-84978765486

(徳島大学機関リポジトリ: 112384, DOI: 10.1155/2016/2581876, PubMed: 27413739, Elsevier: Scopus) Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Hiroyuki Mizuguchi, Takashi Amoh, Satoshi Ogino, Takashi Matsuo, Yoichiro Miyake, Hiroyuki Fukui and Yoshiki Kashiwada :
Anti-bacterial and anti-inflammatory effects of ethanol extract from Houttuynia cordata poultice.,
Bioscience, Biotechnology, and Biochemistry, 2016.

(要約): Houttuynia cordata (HC) has been commonly used as many traditional remedies in local areas of Japan. Although many pharmacological activities of HC have been reported, the mechanism underlying the effect of HC remains unknown. We conducted the interview survey in Japan to verify how HC was actually used. The interview survey revealed that HC poultice (HCP) prepared from smothering fresh leaves of HC was most frequently used for the treatment of purulent skin diseases including furuncle and carbuncle with high effectiveness. Ethanol extract of HCP (eHCP) showed anti-bacterial effects against methicillin-resistant Staphylococcus aureus (MRSA), and showed an anti-biofilm activity against MRSA. eHCP showed dose-dependent inhibition of S. aureus lipoteichoic acid (LTA)-induced interleukin-8 and CCL20 production in human keratinocyte without any cytotoxicity. These results suggest that HCP is effective for skin abscess and its underlying mechanism might be the complicated multiple activities for both bacteria and host cells.
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09168451.2016.1151339
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27023331; ● Search Scopus @ Elsevier (PMID): 27023331; ● Search Scopus @ Elsevier (DOI): 10.1080/09168451.2016.1151339

(DOI: 10.1080/09168451.2016.1151339, PubMed: 27023331) Tsuyoshi Miyagawa, Tsuyoshi Fujita, Hiromichi Yumoto, Tetsuya Yoshimoto, Mikihito Kajiya, Kazuhisa Ouhara, Shinji Matsuda, Hideki Shiba, Takashi Matsuo and Hidemi Kurihara :
Azithromycin recovers reductions in barrier function in human gingival epithelial cells stimulated with tumor necrosis factor-α,
Archives of Oral Biology, Vol.62, 64-69, 2016.

(要約): The gingival epithelium plays an important role in protecting against the invasion of periodontal pathogens, and the permeability of gingival epithelial cells has been implicated in the initiation of periodontitis. Azithromycin (AZM) has been used in the treatment of chronic inflammatory airway diseases because it regulates cell-cell contact in airway epithelial cells. Therefore, AZM may also regulate barrier function in gingival epithelial cells. In the present study, we examined the effects of AZM on the permeability of human gingival epithelial cells (HGEC) under inflammatory conditions in vitro. HGEC were stimulated by tumor necrosis factor-α (TNF-α) in the presence of AZM or p38 MAP kinase and ERK inhibitors. Permeability was assessed based on transepithelial electrical resistance (TER). The expression of E-cadherin, phosphorylated p38 MAP kinase, and ERK was analyzed by Western blotting. TNF-α decreased TER in HGEC, and AZM and the p38 MAP kinase and ERK inhibitors recovered this decrease. AZM inhibited the phosphorylation of ERK and p38 MAP kinase in TNF-α-stimulated HGEC. Furthermore, AZM recovered the decrease in E-cadherin expression in HGEC stimulated with TNF-α. These results suggested that AZM regulated gingival epithelial permeability through p38 MAP kinase and ERK signaling, and may contribute to suppress the inflammation in gingival tissue.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.archoralbio.2015.11.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26655749; ● Summary page in Scopus @ Elsevier: 2-s2.0-84949193967

(DOI: 10.1016/j.archoralbio.2015.11.015, PubMed: 26655749, Elsevier: Scopus) Hiromichi Yumoto, Kouji Hirao, Toshihiko Tominaga, Naoki Bando, Kanako Takahashi and Takashi Matsuo :
Electromagnetic wave irradiation promotes osteoblastic cell proliferation and up-regulates growth factors via activation of the ERK1/2 and p38 MAPK pathways,
Cellular Physiology and Biochemistry, Vol.35, No.2, 601-615, 2015.

(要約): Periodontitis with bone resorption is caused by inflammatory reactions to bacterial infection. We recently reported that electromagnetic wave irradiation (EMWI) has bactericidal effects. However, the effects of EMWI on periodontal tissues remain unclear. This study was aimed to investigate the effects of EMWI on osteoblasts. Osteoblastic cells MC3T3-E1 were treated with EMWI (500-1,000 kHz, 5 times, 1 sec/time). Cell growth and cytotoxicity were determined by cell proliferation assays and measurement of lactate dehydrogenase release, respectively. Gene expression and protein production of growth factors were analyzed using real-time PCR and ELISA, respectively. EMWI-activated cellular signal transduction pathways were investigated by immunoblotting and blocking assay with specific inhibitors. Osteoblasts proliferation was significantly enhanced 3 days after EMWI and no cytotoxicity was observed. EMWI up-regulated various growth factors, such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). EMWI induced ERK1/2, p38 MAPK and SAPK/JNK phosphorylation within 5 min, and the production of PDGF-ΑΑ and VEGF was partially reduced by MAPK-specific inhibitor. These findings demonstrated that EMWI increases osteoblastic cell activity and the expression of growth factors via ERK1/2 and p38 MAPK pathways and suggested that EMWI may be beneficial to bone tissue repair such as periodontitis.
(キーワード): Animals / アポトーシス (apoptosis) / Cell Line / Cell Proliferation / Electromagnetic Radiation / MAP Kinase Signaling System / Mice / Mice, Inbred C57BL / Osteoblasts / リン酸化 (phosphorylation) / Platelet-Derived Growth Factor / Up-Regulation / Vascular Endothelial Growth Factor A
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000369722
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25612851; ● Summary page in Scopus @ Elsevier: 2-s2.0-84921876910

(DOI: 10.1159/000369722, PubMed: 25612851, Elsevier: Scopus) Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Modulatory roles of interferon-γ through indoleamine 2, 3-dioxygenase induction in innate immune response of dental pulp cells.,
Journal of Endodontics, Vol.40, No.9, 1382-1387, 2014.

(要約): Marked infiltration of inflammatory cells such as activated T cells producing interferon-γ (IFN-γ) is observed in severe pulpitis. However, the roles of IFN-γ in the innate immune response of dental pulp have not been reported. Indoleamine 2, 3-dioxygenase (IDO) is a regulator of immune responses, and the IDO expression is induced by IFN-γ in many cells whose expression in dental pulp is unknown. The purpose of this study was to determine the role of IFN-γ in the immune response through microbial pattern recognition receptors (PRRs) such as Toll-like receptors or nucleotide-binding oligomerization domain-like receptors on the production of proinflammatory cytokines such as CXCL10 and interleukin (IL)-6 and the expression of IDO in cultured human dental pulp cells (HDPCs). HDPCs were established from explant cultures of healthy pulp tissues. CXCL10 and IL-6 production was determined using enzyme-linked immunosorbent assay. Confirmation of IDO localization in dental pulp tissues was examined using immunohistochemistry. IDO expression in HDPCs was analyzed by immunoblot. IFN-γ significantly up-regulated CXCL10 and IL-6 production in the HDPCs stimulated with ligands for PRRs in a concentration-dependent manner. The expression of IDO was detected in inflamed pulp tissue. In addition, IFN-γ in combination with the PRR ligands enhanced IDO expression in HDPCs compared with IFN-γ alone. Moreover, CXCL10 production in IFN-γ-stimulated HDPCs was inhibited by an IDO inhibitor. This study showed the synergistic effects by IFN-γ on cytokine production and IDO expression in HDPCs, suggesting that IFN-γ may modulate the innate immune response of dental pulp.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.joen.2014.03.018
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25146019; ● Summary page in Scopus @ Elsevier: 2-s2.0-85027943562

(DOI: 10.1016/j.joen.2014.03.018, PubMed: 25146019, Elsevier: Scopus) Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Tsuyoshi Miyazaki, Nobuyuki Yamamoto, Koji Miyamoto, Keiji Murakami, Natsumi Fujiwara, Takashi Matsuo and Yoichiro Miyake :
Anti-inflammatory and protective effects of 2-methacryloyloxyethyl phosphorylcholine polymer on oral epithelial cells.,
Journal of Biomedical Materials Research. Part A, Vol.103, No.2, 555-563, 2014.

(要約): Periodontitis is a chronic inflammatory disease initiated by a microbial biofilm formed in the periodontal pocket. Gingival epithelium plays important roles as the first physical barrier to bacterial invasion and in orchestrating the innate immune reaction via toll-like receptors (TLRs), which recognize various bacterial products, and maintaining its function. Newly developed oral care products to inhibit bacterial adherence, subsequent inflammatory reaction and protect the gingival epithelium are expected. We previously reported that 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer coating decreased bacterial adhesion to human oral keratinocytes, RT-7, and mouth-rinsing with MPC-polymer inhibited the increase of oral bacteria. In this study, regarding the possibility of MPC-polymer application for preventing the adherence of periodontal pathogen, subsequent inflammatory reaction and protection of gingival epithelium, we examined the effects of MPC-polymer on the adherence of Porphyromonas gingivalis, major periodontitis-related pathogen, and TLR2 ligand to RT-7 and subsequent interleukin (IL)-8 production. MPC-polymer treatment significantly reduced P. gingivalis adherence by 44% and TLR2-mediated IL-8 production by blocking the binding of its specific-ligand in a concentration-dependent manner. Furthermore, MPC-polymer pretreatment protected RT-7 from injury by chemical irritants, cetylpyridinium chloride. These findings suggest that MPC-polymer is potentially useful for oral care to prevent oral infection and to maintain oral epithelial function. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2014.
(キーワード): Anti-Inflammatory Agents / Bacteroidaceae Infections / Cell Line, Transformed / Epithelial Cells / Humans / Mouth / 歯周病 (periodontitis) / Phosphorylcholine / Polymethacrylic Acids / Porphyromonas gingivalis
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.35201
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24753309; ● Summary page in Scopus @ Elsevier: 2-s2.0-84924401882

(DOI: 10.1002/jbm.a.35201, PubMed: 24753309, Elsevier: Scopus) Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa,
Bacterial Adherence & Biofilm, Vol.27, 45-47, 2014. B Sapaar, A Nur, Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, T Amoh, Takashi Matsuo, Tetsuo Ichikawa and Yoichiro Miyake :
Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation.,
Journal of Applied Microbiology, Vol.116, No.6, 1531-1542, 2014.

(要約): The aim of this study was to investigate the effects of genomic DNA purified from Candida albicans and pneumonia-related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, on in vitro biofilm formation and morphological change of 3 Candida species (C. albicans, C. glabrata, and C. tropicalis). Biofilm formation was evaluated by the crystal violet assay and colony-forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (<1·0 μg ml(-1)) significantly increased biofilm mass of all three Candida species. In contrast, the addition of DNA at a high concentration (10 μg ml(-1)) decreased the biofilm mass. Interestingly, the formation of hyphae in a dense network of yeast cells was observed in C. albicans biofilms exposed to a low concentration of DNA (<1·0 μg ml(-1)). These findings demonstrated that extracellular DNA (eDNA) plays a crucial role in Candida biofilm formation and suggested that eDNA may induce the morphological transition from yeast to hyphal growth form during C. albicans biofilm development. A novel therapy targeting eDNA may be applicable for Candida infection to decrease biofilm formation and hyphal formation.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.12483
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24661775; ● Summary page in Scopus @ Elsevier: 2-s2.0-84900806168

(DOI: 10.1111/jam.12483, PubMed: 24661775, Elsevier: Scopus) Wang Yiwei, 湯本浩通, Liu Dali, 松尾敬志, Shu Rong :
The comparison of the ability of polysaccharide from different Porphyromonas gingivalis in inducing THP-1 cells to produce cytokines,
Chinese Journal of Stomatology, Vol.49, No.2, 78-83, 2014年.

(要約): To compare the ability of the polysaccharide from various Porphyromonas gingivalis (Pg) type and clinical strains in inducing THP-1 cells to produce cytokines interleukin(IL)-1β, IL-8, and tumor necrosis factor(TNF)-α, in order to analyze the immunogenicity of Pg polysaccharide components and the virulence-associated factors of this periodontal pathogen. The bacterial polysaccharide was extracted from high virulent Pg strains, W83, SJD2, SJD12 and low virulent Pg, ATCC33277, SJD4, SJD5, and SJD11 by phenol-water extraction. The extracted polysaccharide was used to stimulate the THP-1 cells with different simulation periods and doses. The level of the cytokines, including IL-1β,IL-8 and TNF-α in the cell culture suspension was measured by enzyme-linked immunosorbent assay(ELISA). The polysaccharide extraction of Pg strains was composed of lipopolysaccharide(LPS) and capsular polysaccharide. The secretion of IL-1β, IL-8 and TNF-α, produced by the THP-1 cells showed in a time- and dose-dependent manner in the medium containing 10% fetal bovine serum. The level of these cytokines of the high virulent strains was higher than that of the low virulent strains in medium containing 1% fetal bovine serum.Four hours after stimulation with polysaccharide extracted from high virulent strains, the levels of IL-1β,IL-8, and TNF-α in the cell suspension were (1 639 ± 497), (1 648 ± 513) and (140 ± 48) µg/L, respectively, whereas for low virulent strains, the levels of IL-1β, IL-8, and TNF-α were (773 ± 382), (892 ± 400) and (67 ± 33) µg/L, respectively. Polysaccharide extracted from Pg could induced the THP-1 cells to secrete the cytokines of IL-1β, IL-8 and TNF-α. The level of the cytokines produced by the THP-1 cells associates with the bacterial virulent properties.
(キーワード): Cytokines / Interleukin-1beta / Interleukin-8 / Lipopolysaccharides / Porphyromonas gingivalis / Tumor Necrosis Factor-alpha
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24731418; ● Summary page in Scopus @ Elsevier: 2-s2.0-84929938924

(PubMed: 24731418, Elsevier: Scopus) Dali Liu, Yanbin Zhou, Mariko Naito, Hiromichi Yumoto, Qiang Li, Yoichiro Miyake, Jingping Liang and Rong Shu :
Draft Genome Sequence of Porphyromonas gingivalis Strain SJD2, Isolated from Periodontal Pocket of a Patient with Periodontitis in China,
Genome Announcements, Vol.2, No.1, e01091-13, 2014.

(要約): Porphyromonas gingivalis strain SJD2 was isolated from subgingival plaque of a patient in China with chronic periodontitis. Here, we report the draft genome of this strain, with a size of 2,328,850 bp, average G+C content of 48.3%, and 2,020 predicted protein-coding sequences.
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/genomeA.01091-13
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24385574; ● Summary page in Scopus @ Elsevier: 2-s2.0-84998610618

(DOI: 10.1128/genomeA.01091-13, PubMed: 24385574, Elsevier: Scopus) Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Role of Extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermeius,
Bacterial Adherence & Biofilm, Vol.26, 25-29, 2013. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, D Liu, Kanako Takahashi, Keiji Murakami, Takashi Matsuo, R Shu and Yoichiro Miyake :
Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm.,
Journal of Applied Microbiology, Vol.115, No.1, 260-270, 2013.

(要約): The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA-binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity. Formed biofilm mass was measured with 0·1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml(-1) ) markedly decreased biofilm formation and cell density in biofilms. Colocalization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml(-1) ) purified from Strep. intermedius, other Gram-positive bacteria, Gram-negative bacteria, or human KB cells into the Strep. intermedius culture increased the biofilm mass of all tested strains of Strep. intermedius, wild-type, HLP-downregulated strain and control strains. In contrast, the addition of eDNA (>1 μg ml(-1) ) decreased the biofilm mass of all Strep. intermedius strains. These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity. eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
(キーワード): Biofilms / Cell Line, Tumor / DNA (DNA) / DNA-Binding Proteins / Deoxyribonuclease I / Humans / Streptococcus intermedius
(徳島大学機関リポジトリ): ● Metadata: 106279
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.12202
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23551549; ● Summary page in Scopus @ Elsevier: 2-s2.0-84879085342

(徳島大学機関リポジトリ: 106279, DOI: 10.1111/jam.12202, PubMed: 23551549, Elsevier: Scopus) Hiromichi Yumoto, Toshihiko Tominaga, Kouji Hirao, Tomoko Kimura, Kanako Takahashi, Takashi Sumitomo, Naoki Bando and Takashi Matsuo :
Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation,
Journal of Applied Microbiology, Vol.113, No.1, 181-191, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2672.2012.05307.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22507081; ● Search Scopus @ Elsevier (PMID): 22507081; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2672.2012.05307.x

(DOI: 10.1111/j.1365-2672.2012.05307.x, PubMed: 22507081) Jing Li, Katsuhiko Hirota, Takaharu Goto, Hiromichi Yumoto, Yoichiro Miyake and Tetsuo Ichikawa :
Biofilm formation of Candida albicans on implant overdenture materials and its removal.,
Journal of Dentistry, Vol.40, No.8, 686-692, 2012.

(要約): The purposes of this study were to clarify the surface characteristics of various implant overdenture materials and the capabilities of Candida albicans adherence and biofilm formation on these surfaces, and to investigate the role of salivary mucin in biofilm formation. Seven commonly used implant and restorative materials were assessed. The surface roughness averages of all materials were limited to 0.07-0.10μm. Contact angles and salivary mucin absorption were measured. After 90-min initial adhesion and 2-day biofilm formation, the amounts of C. albicans were determined by counting colony-forming units and the morphological characteristics were observed by scanning electron microscopy (SEM). The effects of saliva coating and the influences of material surface property on initial adhesion, biofilm formation and its removability were analysed by univariate two-way analysis of variance and multiple linear regression analysis. Surface contact angle of materials, the index of hydrophobicity, was found to be correlated positively with initial adhesion and biofilm formation of C. albicans. A negative correlation between mucin absorption and removability of Candida biofilm indicates that mucin plays an important role in biofilm formation and its rigidity. SEM observation also revealed fewer Candida cells on saliva-coated Ti than on saliva-coated hydroxyapatite or acrylic resin. The materials with different hydrophobic property and compositions display diverse manners of salivary mucin absorption, initial adhesion and biofilm formation. The hydrophobic materials encourage enhanced initial adhesion, subsequently resulting in the active biofilm formation. Mucin has decisive effects on Candida immobilization and biofilm development on the materials. Surface hydrophilic property and composition of materials and salivary proteins, especially mucin, affect the process of Candida biofilm formation and influence the amount and rigidity of formed biofilm. The present data may be applied as a reference for selecting materials in implant overdenture treatment from a microbiological point of view.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jdent.2012.04.026
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22580351; ● Search Scopus @ Elsevier (PMID): 22580351; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jdent.2012.04.026

(DOI: 10.1016/j.jdent.2012.04.026, PubMed: 22580351) Tsuyoshi Fujita, Hiromichi Yumoto, Hideki Shiba, Kazuhisa Ouhara, Tsuyoshi Miyagawa, Takayoshi Nagahara, Shinji Matsuda, Hiroyuki Kawaguchi, Takashi Matsuo, Shinya Murakami and Hidemi Kurihara :
Irsogladine maleate regulates epithelial barrier function in TNF--stimulated human gingival epithelial cells,
Journal of Periodontal Research, Vol.47, No.1, 55-61, 2012.

(要約): As epithelial cells function as a mechanical barrier, the permeability of the gingival epithelial cell layer indicates a defensive capability against invasion by periodontal pathogens. We have reported the expression of claudin-1 and E-cadherin, key regulators of permeability, in the gingival junctional epithelium. Irsogladine maleate (IM) is a medication for gastric ulcers and also regulates Aggregatibacter actinomycetemcomitans-stimuated chemokine secretion and E-cadherin expression in gingival epithelium. In this study, we have further investigated the effects of IM on the barrier functions of gingival epithelial cells under inflammatory conditions. We examined the permeability, and the expression of claudin-1 and E-cadherin, in human gingival epithelial cells (HGECs) stimulated with tumor necrosis factor (TNF)-α, with or without IM. TNF-α increased the permeability of HGECs, and IM abolished the increase. TNF-α reduced the expression of E-cadherin in HGECs, and IM reversed the reduction. In addition, immunofluorescence staining showed that TNF-α disrupted claudin-1 expression in HGECs, and IM reversed this effect. The results suggest that IM reverses the TNF-α-induced disruption of the gingival epithelial barrier by regulating E-cadherin and claudin-1.
(キーワード): Adult / Blotting, Western / Cadherins / Cell Culture Techniques / Cell Line / Cell Membrane Permeability / Claudin-1 / Electric Impedance / Epithelial Attachment / Epithelial Cells / Female / Fluorescein / Fluorescent Antibody Technique / Fluorescent Dyes / Gingiva / Humans / Male / Membrane Proteins / Real-Time Polymerase Chain Reaction / Tight Junctions / Triazines / Tumor Necrosis Factor-alpha / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0765.2011.01404.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21895660; ● Search Scopus @ Elsevier (PMID): 21895660; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2011.01404.x

(DOI: 10.1111/j.1600-0765.2011.01404.x, PubMed: 21895660) 坂東直樹, 富永敏彦, 湯本浩通, 住友孝史, 平尾早希, 平尾功治, 松尾敬志 :
電磁波照射の歯内療法への応用ーEMAT (Electro-Magnetic Apical Treatment)ー,
日本歯内療法学会雑誌, Vol.32, No.3, 184-200, 2011年.

(キーワード): electro-magnetic wave / periapical lesion / non-surgical treatment / refractory periapical periodontitis
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680166084096

(CiNii: 1390282680166084096) Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Kanako Takahashi, Hiromichi Yumoto and Takashi Matsuo :
Roles of dental pulp fibroblasts in the recognition of bacterium-related factors and subsequent development of pulpitis,
Japanese Dental Science Review, Vol.47, No.2, 161-166, 2011.

(キーワード): Toll-like receptor (TLR) / Nucleotide-binding oligomerization domain (NOD) / Dental pulp fibroblast / Cytokine / Pulpitis
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jdsr.2011.02.001
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1360565165959499264; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jdsr.2011.02.001

(DOI: 10.1016/j.jdsr.2011.02.001, CiNii: 1360565165959499264) Katsuhiko Hirota, Hiromichi Yumoto, K Miyamoto, N Yamamoto, Keiji Murakami, Yumi Hoshimo, Takashi Matsuo and Yoichiro Miyake :
MPC-Polymer Reduces Adherence and Biofilm Formation by Oral Bacteria.,
Journal of Dental Research, Vol.90, No.7, 900-905, 2011.

(要約): Oral biofilms such as dental plaque cause dental caries and periodontitis, as well as aspiration pneumonia and infectious endocarditis by translocation. Hence, the suppression of oral biofilm formation is an issue of considerable importance. Mechanical removal, disinfectants, inhibition of polysaccharide formation, and artificial sugar have been used for the reduction of oral biofilm. From the viewpoint of the inhibition of bacterial adherence, we investigated whether aqueous biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer can reduce streptococcal colonization and biofilm formation. We examined the effects of MPC-polymer on streptococcal adherence to saliva-coated hydroxyapatite and oral epithelial cells, and the adherence of Fusobacterium nucleatum to streptococcal biofilm. MPC-polymer application markedly inhibited both the adherence and biofilm formation of Streptococcus mutans on saliva-coated hydroxyapatite and streptococcal adherence to oral epithelial cells, and reduced the adherence of F. nucleatum to streptococcal biofilms. A small-scale clinical trial revealed that mouthrinsing with MPC-polymer inhibited the increase of oral bacterial numbers, especially of S. mutans. These findings suggest that MPC-polymer is a potent inhibitor of bacterial adherence and biofilm development, and may be useful to prevent dental-plaque-related diseases. (UMIN Clinical Trial Registry UMIN000003471). Abbreviations: QOL, quality of life; MPC, 2-methacryloyloxyethyl phosphorylcholine; BHI, brain-heart infusion broth; PBS, phosphate-buffered saline; SEM, scanning electron microscopy; CFU, colony-forming unit.
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/0022034511402996
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21447697; ● Summary page in Scopus @ Elsevier: 2-s2.0-84884211413

(DOI: 10.1177/0022034511402996, PubMed: 21447697, Elsevier: Scopus) J Li, Katsuhiko Hirota, Hiromichi Yumoto, Takashi Matsuo, Yoichiro Miyake and Tetsuo Ichikawa :
Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms.,
Journal of Applied Microbiology, Vol.109, No.6, 2183-2190, 2010.

(要約): The germicidal effects of UVA-LED irradiation (365 nm, 0·28 mW cm(-2) , in pulsed or continuous mode) on Candida albicans or Escherichia coli biofilms were evaluated by determining colony-forming units. The morphological change of microbial cells in biofilms was observed using scanning electron microscopy. After 5-min irradiation, over 90% of viable micro-organisms in biofilms had been killed, and pulsed irradiation (1-1000 Hz) had significantly greater germicidal ability than continuous irradiation. Pulsed irradiation (100 Hz, 60 min) almost completely killed micro-organisms in biofilm (>99·9%), and 20-min irradiation greatly damaged both microbial species. Interestingly, few hyphae were found in irradiated Candida biofilms. Moreover, mannitol treatment, a scavenger of hydroxyl radicals (OH(•) ), significantly protected viable micro-organisms in biofilms from UVA-LED irradiation.
(キーワード): Biofilms / Candida albicans / Colony Count, Microbial / Disinfection / 大腸菌 (Escherichia coli) / Free Radical Scavengers / Hydroxyl Radical / Mannitol / Microbial Viability / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2672.2010.04850.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20854456; ● Summary page in Scopus @ Elsevier: 2-s2.0-78449262889

(DOI: 10.1111/j.1365-2672.2010.04850.x, PubMed: 20854456, Elsevier: Scopus) Kouji Hirao, Hiromichi Yumoto, Tadashi Nakanishi, Kayo Mukai, Kanako Takahashi, Daisuke Takegawa and Takashi Matsuo :
Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells.,
Life Sciences, Vol.86, No.17-18, 654-660, 2010.

(要約): These findings suggest that catechins might be useful therapeutically as an anti-inflammatory modulator of dental pulpal inflammation.
(キーワード): 抗炎症剤 / カテキン (catechin) / Cells, Cultured / 歯髄細胞 / Enzyme-Linked Immunosorbent Assay / Fibroblasts / Humans / Inflammation / Inflammation Mediators / Ligands / Mitogen-Activated Protein Kinases / Phosphorylation / Receptors, Pattern Recognition / Reverse Transcriptase Polymerase Chain Reaction / Signal Transduction / Streptococcus / Tea / Toll-Like Receptor 2 / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2010.02.017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20176036; ● Search Scopus @ Elsevier (PMID): 20176036; ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2010.02.017

(DOI: 10.1016/j.lfs.2010.02.017, PubMed: 20176036) Tadashi Nakanishi, Kayo Mukai, Hiromichi Yumoto, Kouji Hirao, Yoshitaka Hosokawa and Takashi Matsuo :
Anti-inflammatory effect of catechin on cultured dental pulp cells affected by bacteria-derived factors,
European Journal of Oral Sciences, Vol.118, No.2, 145-150, 2010.

(要約): Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis.
(キーワード): Anti-Inflammatory Agents / Antioxidants / Catechin / Cells, Cultured / Dental Pulp / Escherichia coli / Flow Cytometry / Humans / Intercellular Adhesion Molecule-1 / Interleukin-6 / Interleukin-8 / Lipopolysaccharides / Peptidoglycan / Reverse Transcriptase Polymerase Chain Reaction / Staphylococcus aureus / Time Factors / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0722.2010.00714.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20487003; ● Search Scopus @ Elsevier (PMID): 20487003; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0722.2010.00714.x

(DOI: 10.1111/j.1600-0722.2010.00714.x, PubMed: 20487003) Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Proinflammatroy effects of muramyldipeptide on human gingival fibroblasts,
Journal of Periodontal Research, Vol.45, No.2, 193-199, 2010.

(要約): Because human gingival fibroblasts (HGFs) are the predominant cells in periodontal tissues, we hypothesized that HGFs are contributed to receptors for components of bacteria. In this study, we focused on expression and function of nucleotide binding oligomerization domain 2 (NOD2) in HGFs, which is a mammalian cytosolic pathogen recognition molecule. Expression of NOD2 in HGFs was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry. Production of interleukin (IL)-6, IL-8, cc chemokine ligand2, cxc chemokine ligand10 (CXCL10) and CXCL11 from HGFs was examined by enzyme-linked immunosorbent assay (ELISA). We used RT-PCR and immunohistochemistry to detect the NOD2 expression in human gingival tissues. We found clear NOD2 expression in HGFs. Upon stimulation with NOD2 agonist, muramyldipeptide (MDP), production of proinflammatory cytokines was enhanced. Moreover, MDP-induced production of proinflammatory cytokines was inhibited in a different manner by mitogen-activated protein kinase inhibitors and phosphatidylinositol 3-kinase inhibitor. Furthermore, MDP enhanced CXCL10 and CXCL11 productions by tumor necrosis factor-alpha (TNF-alpha)- or interferon-gamma (IFN-gamma)-stimulated HGFs, although MDP alone did not induce these chemokines. TNF-alpha and IFN-gamma increased NOD2 expression in HGFs. In addition, we detected NOD2 expression in mononuclear cells and HGFs in periodontally diseased tissues. These findings indicate that MDP which induces production of cytokines and chemokines from HGFs is related to the pathogenesis of periodontal disease.
(キーワード): Acetylmuramyl-Alanyl-Isoglutamine / Adult / Anthracenes / Calcium-Calmodulin-Dependent Protein Kinases / Cells, Cultured / Chemokine CCL2 / Chemokine CXCL10 / Chemokine CXCL11 / Chromones / Chronic Periodontitis / Enzyme Inhibitors / Female / Fibroblasts / Flavonoids / Gingiva / Humans / Imidazoles / Inflammation Mediators / Interferon-gamma / Interleukin-6 / Interleukin-8 / JNK Mitogen-Activated Protein Kinases / Male / Middle Aged / Morpholines / Nod2 Signaling Adaptor Protein / Periodontal Attachment Loss / Periodontal Pocket / Phosphatidylinositol 3-Kinases / Pyridines / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0765.2009.01217.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20470259; ● Search Scopus @ Elsevier (PMID): 20470259; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2009.01217.x

(DOI: 10.1111/j.1600-0765.2009.01217.x, PubMed: 20470259) Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi, Kayo Mukai, Tadashi Nakanishi and Takashi Matsuo :
Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts.,
Journal of Dental Research, Vol.88, No.8, 762-767, 2009.

(要約): Pulp fibroblasts express various pro-inflammatory mediators leading to marked infiltration of inflammatory cells in the progression of pulpitis. We hypothesized that pulp fibroblasts play roles in the recognition of invaded caries-related bacteria and the subsequent innate immune responses. We found clear expressions of TLR2, NOD1, and NOD2 and a faint expression of TLR4 in human dental pulp fibroblasts (HDPF) by RT-PCR and flow cytometry. We also observed that various pro-inflammatory mediators, including cytokines, chemokines, adhesion molecules, prostaglandin E(2) and its key enzyme COX-2, not iNOS or caspase-1, were markedly up-regulated by stimulation with these TLR and NOD agonists. More over, the NOD2 agonist acted synergistically with the TLR2, not the TLR4, agonist to stimulate the production of pro-inflammatory mediators in HDPF. These findings indicate that TLR2, TLR4, NOD2, and NOD1 in HDPF are functional receptors, and NOD2 is a modulator of signals transmitted through TLR2 in pulpal immune responses, leading to progressive pulpitis.
(キーワード): Acetylmuramyl-Alanyl-Isoglutamine / Apoptosis / Cells, Cultured / Chemokine CCL2 / Chemokine CXCL10 / Cyclooxygenase 2 / Dental Pulp / Diaminopimelic Acid / Dinoprostone / Escherichia coli / Fibroblasts / Humans / Inflammation Mediators / Interleukin-6 / Interleukin-8 / Lipopeptides / Lipopolysaccharides / Nod1 Signaling Adaptor Protein / Nod2 Signaling Adaptor Protein / Pulpitis / Signal Transduction / Streptococcus mutans / Toll-Like Receptor 2 / Toll-Like Receptor 4 / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/0022034509341779
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19734466; ● Search Scopus @ Elsevier (PMID): 19734466; ● Search Scopus @ Elsevier (DOI): 10.1177/0022034509341779

(DOI: 10.1177/0022034509341779, PubMed: 19734466) Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Taniguchi Tomonori, Yamamoto Akitake, Tsuneko Ono, Takashi Matsuo and Yoichiro Miyake :
Heterologous expression of a histone-like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E. coli,
FEMS Microbiology Letters, Vol.288, No.1, 68-75, 2008.

(要約): Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli.
(キーワード): Bacterial Proteins / Cell Nucleolus / Cloning, Molecular / DNA, Bacterial / DNA, Superhelical / 大腸菌 (Escherichia coli) / 遺伝子発現 (gene expression) / Histones / Streptococcus intermedius
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1574-6968.2008.01327.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18761672; ● Summary page in Scopus @ Elsevier: 2-s2.0-52649125775

(DOI: 10.1111/j.1574-6968.2008.01327.x, PubMed: 18761672, Elsevier: Scopus) Kanako Takahashi, Tadashi Nakanishi, Hiromichi Yumoto, Tomohiko Adachi and Takashi Matsuo :
CCL20 production is induced in human dental pulp upon stimulation by Streptococcus mutans and pro-inflammatory cytokines,
Oral Microbiology and Immunology, Vol.23, No.4, 320-327, 2008.

(要約): Pulpitis is characterized by the marked infiltration of inflammatory cells in response to an invasion of caries-related bacteria. It is well known that chemokines regulate the trafficking of lymphocytes, and CC chemokine ligand 20 (CCL20) has been recently shown to play a crucial role in the recruitment of memory T cells and immature dendritic cells into inflammatory lesions. We previously reported that CCL20 was mainly expressed in microvascular endothelial cells and macrophages that accumulated in inflamed pulp tissues and that its specific receptor, CCR6, was expressed on infiltrated lymphocytes. However, the mechanism of CCL20 expression remains unclear. In this study, we investigated the expression of CCL20 in monocytes/macrophages, endothelial cells, and pulpal fibroblasts after stimulation with Streptococcus mutans, a representative of caries-related bacteria, or proinflammatory cytokines. CCL20 messenger RNA was detected by reverse transcription-polymerase chain reaction in inflamed pulp, but not in clinically normal pulp. By enzyme-linked immunosorbent assay, S. mutans induced a human monocytic cell line, differentiated macrophage-like THP-1 cells, and human umbilical vein endothelial cells (HUVEC) to produce an increased amount of CCL20. Lipoteichoic acid from S. mutans also elicited CCL20 production by HUVEC. Moreover, CCL20 production from pulpal fibroblasts was increased by stimulation with inetrleukin-1beta and tumor necrosis factor-alpha. Our results indicate that CCL20 expression is induced by stimulation with caries-related bacteria that have invaded deeply into the dentinal tubules as well as by proinflammatory cytokines in the inflamed pulpal lesions. It may be involved in the progression of pulpitis via accumulation of inflammatory cells.
(キーワード): Adult / Aged / Cell Differentiation / Cell Line / Cells, Cultured / Chemokine CCL20 / Cytokines / Dental Pulp / Endothelial Cells / Endothelium, Vascular / Female / Fibroblasts / Humans / Inflammation Mediators / Intercellular Adhesion Molecule-1 / Interleukin-1beta / Interleukin-8 / Lipopolysaccharides / Macrophages / Male / Middle Aged / Monocytes / Pulpitis / Streptococcus mutans / Teichoic Acids / Tumor Necrosis Factor-alpha / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1399-302X.2008.00431.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18582332; ● Search Scopus @ Elsevier (PMID): 18582332; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1399-302X.2008.00431.x

(DOI: 10.1111/j.1399-302X.2008.00431.x, PubMed: 18582332) Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
The essentiality and involvement of Streptococcus intermedius histone-like DNA-binding protein in bacterial viability and normal growth,
Molecular Microbiology, Vol.68, No.5, 1268-1282, 2008.

(要約): Streptococcus intermedius histone-like DNA-binding protein (Si-HLP) is a homodimeric protein and, conserved with Escherichia coli HU, a well-documented nucleoid-associated protein (NAP). In E. coli, HU plays important roles as both structural and regulatory factors, but it is not essential for E. coli viability. Streptococcal HLP has been found to bind host cells and induce cytokine production, but its physiological role remains poorly defined. In the present study, using gene insertion knockout and tetracycline-regulated antisense RNA expression techniques, we determined whether Si-HLP is essential for bacterial viability and normal growth in S. intermedius. The Si-HLP-downregulated S. intermedius strain showed alterations in its morphology and surface properties. Downregulation of Si-HLP led to an expanded nucleoid to fill the intracellular space. Transcription levels of several genes, including virulence-associated factors, were found to be activated or repressed in the antisense Si-hlp RNA-expressing strain by real-time PCR and reverse-transcription PCR. Collectively, these data suggest that Si-HLP serves as an essential NAP governing the nucleoid architecture and controlling the gene transcription profile in S. intermedius.
(キーワード): Bacterial Proteins / DNA-Binding Proteins / Escherichia coli Proteins / Gene Expression Regulation, Bacterial / Histones / Microbial Viability / Streptococcus intermedius / Transcription, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2958.2008.06232.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18410499; ● Summary page in Scopus @ Elsevier: 2-s2.0-43749117518

(DOI: 10.1111/j.1365-2958.2008.06232.x, PubMed: 18410499, Elsevier: Scopus) Takashi Ukai, Hiromichi Yumoto, Frank Gibson C III and Caroline A Genco :
Macrophage-elicited osteoclastogenesis in response to bacterial stimulation requires Toll-like receptor 2-dependent tumor necrosis factor-alpha production,
Infection and Immunity, Vol.76, No.2, 812-819, 2008.

(要約): The receptor activator of NF-kappaB ligand (RANKL) and the proinflammatory cytokines are believed to play important roles in osteoclastogenesis. We recently reported that the innate immune recognition receptor, Toll-like receptor 2 (TLR2), is crucial for inflammatory bone loss in response to infection by Porphyromonas gingivalis, the primary organism associated with chronic inflammatory periodontal disease. However, the contribution of macrophage-expressed TLRs to osteoclastogenesis has not been defined. In this study, we defined a requirement for TLR2 in tumor necrosis factor-alpha (TNF-alpha)-elicited osteoclastogenesis in response to exposure to P. gingivalis. Culture supernatant (CS) fluids from P. gingivalis-stimulated macrophages induced bone marrow macrophage-derived osteoclastogenesis. This activity was dependent on TNF-alpha and occurred independently of RANKL, interleukin-1beta (IL-1beta), and IL-6. CS fluids from P. gingivalis-stimulated TLR2(-/-) macrophages failed to express TNF-alpha, and these fluids induced significantly less osteoclast formation compared with that of the wild-type or the TLR4(-/-) macrophages. In addition, P. gingivalis exposure induced up-regulation of TLR2 expression on the cell surface of macrophages, which was demonstrated to functionally react to reexposure to P. gingivalis, as measured by a further increase in TNF-alpha production. These results demonstrate that macrophage-dependent TLR2 signaling is crucial for TNF-alpha-dependent/RANKL-independent osteoclastogenesis in response to P. gingivalis infection. Furthermore, the ability of P. gingivalis to induce the cell surface expression of TLR2 may contribute to the chronic inflammatory state induced by this pathogen.
(キーワード): Animals / Bacteroidaceae Infections / Bone Resorption / Macrophages / Male / Mice / Mice, Inbred C57BL / Mice, Knockout / Osteoclasts / Porphyromonas gingivalis / Toll-Like Receptor 2 / Toll-Like Receptor 4 / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.01241-07
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17998311; ● Search Scopus @ Elsevier (PMID): 17998311; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.01241-07

(DOI: 10.1128/IAI.01241-07, PubMed: 17998311) Dali Liu, Hiromichi Yumoto, Katsuhiko Hirota, Keiji Murakami, Kanako Takahashi, Kouji Hirao, Takashi Matsuo, Kazuto Ohkura, Hideaki Nagamune and Yoichiro Miyake :
Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via action of ERK1/2 and JNK pathways,
Cellular Microbiology, Vol.10, No.1, 262-276, 2008.

(要約): Streptococcus intermedius is a commensal associated with serious, deep-seated purulent infections in major organs, such as the brain and liver. Histone-like DNA binding protein (HLP) is an accessory architectural protein in a variety of bacterial cellular processes. In this study, we investigated the mechanisms of pro-inflammatory cytokine inductions in THP-1 cells by stimulation with recombinant HLP of S. intermedius (rSi-HLP). rSi-HLP stimulation-induced production of pro-inflammatory cytokines (IL-8, IL-1 beta and TNF-alpha) occurred in a time- and dose-dependent manner. In contrast with the heat-stable activity of DNA binding, the induction activity of rSi-HLP was heat-unstable. In subsequent studies, rSi-HLP acted cooperatively with lipoteichoic acid, the synthetic Toll-like receptor 2 agonist, Pam3CSK4, and the cytosolic nucleotide binding oligomerization domain 2 receptor agonist, muramyldipeptide. Furthermore, Western blot and blocking assays with specific inhibitors showed that rSi-HLP stimulation induced the activation of cell signal transduction pathways, extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). In addition to its physiological role in bacterial growth through DNA binding, these results indicate that Si-HLP can trigger a cascade of events that induce pro-inflammatory responses via ERK1/2 and JNK signal pathways, and suggest that bacterial HLP may contribute to the activation of host innate immunity during bacterial infection.
(キーワード): Acetylmuramyl-Alanyl-Isoglutamine / Bacterial Proteins / Cell Line / Cytokines / DNA, Bacterial / DNA-Binding Proteins / Hot Temperature / Humans / JNK Mitogen-Activated Protein Kinases / Lipopolysaccharides / Mitogen-Activated Protein Kinase 3 / Molecular Sequence Data / Monocytes / Recombinant Proteins / Sequence Analysis, DNA / Signal Transduction / Streptococcus intermedius / Teichoic Acids / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1462-5822.2007.01040.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17883418; ● Search Scopus @ Elsevier (PMID): 17883418; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1462-5822.2007.01040.x

(DOI: 10.1111/j.1462-5822.2007.01040.x, PubMed: 17883418) Xinyan Liu, Takashi Ukai, Hiromichi Yumoto, Michael Davey, Sulip Goswami, Frank Gibson C III and Caroline A Genco :
Toll-like receptor 2 plays a critical role in the progression of atherosclerosis that is independent of dietary lipids,
Atherosclerosis, Vol.196, No.1, 146-154, 2008.

(要約): Toll-like receptors (TLRs), a group of pathogen-associated microbial pattern recognition receptors, play an important role in innate immune signaling and are differentially regulated in chronic inflammatory diseases such as atherosclerosis. However, the involvement of TLRs in the progression of atherosclerosis is still unclear. TLR2 and apolipoprotein E double knockout (Tlr2(-/-)Apoe(-/-)) mice were generated and the progressive formation of atherosclerotic plaque in the aortas was examined in mice fed a normal chow diet. We demonstrate that inactivation of TLR2 resulted in reduced progression of atherosclerosis in both male and female Apoe(-/-) mice. Likewise, TLR2 deficiency resulted in a reduction in lipid accumulation and decreased macrophage recruitment to the aortic sinus, as well as reduced monocyte chemoattractant protein-1 (MCP-1) levels. Furthermore, macrophages isolated from Tlr2(-/-)Apoe(-/-) mice demonstrated significantly reduced MCP-1 production upon stimulation with a TLR2 ligand. However, no differences in acetylated low-density lipoprotein uptake and foam cell formation were observed in macrophages isolated from Tlr2(-/-)Apoe(-/-) mice as compared to Apoe(-/-) mice. TLR2 plays a critical role in the progression of atherosclerosis in Apoe(-/-) mice, which is independent of dietary lipids and macrophage lipid uptake.
(キーワード): Animals / Aorta / Apolipoproteins E / Atherosclerosis / Dietary Fats / Disease Models, Animal / Female / Foam Cells / Inflammation / Male / Mice / Mice, Knockout / Toll-Like Receptor 2
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.atherosclerosis.2007.03.025
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17466307; ● Search Scopus @ Elsevier (PMID): 17466307; ● Search Scopus @ Elsevier (DOI): 10.1016/j.atherosclerosis.2007.03.025

(DOI: 10.1016/j.atherosclerosis.2007.03.025, PubMed: 17466307) Tomohiko Adachi, Tadashi Nakanishi, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Kayo Mukai, Hideaki Nakae and Takashi Matsuo :
Caries-related bacteria and cytokines induce CXCL10 in dental pulp.,
Journal of Dental Research, Vol.86, No.12, 1217-1222, 2007.

(要約): Marked infiltration of inflammatory cells, such as activated T-cells, is observed in the progression of pulpitis; however, little is known about the mechanism of their recruitment into pulpal lesions. It has been recently demonstrated that CXC chemokine ligand 10 (CXCL10) chemoattracts CXC chemokine receptor 3 (CXCR3)-positive activated T-cells. We therefore examined whether CXCL10 is involved in the pathogenesis of pulpitis. CXCL10 mRNA expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Immunostaining results revealed that CXCL10 was detected in macrophages, endothelial cells, and fibroblasts in inflamed dental pulp, and that CXCR3 expression was observed mainly on T-cells. Moreover, cultured dental pulp fibroblasts produced CXCL10 after stimulation with live caries-related bacteria, peptidoglycans, and pro-inflammatory cytokines. In contrast, heat-killed bacteria did not induce CXCL10 secretion. These findings suggest that CXCL10-CXCR3 may play an important role in the pulpal immune response to caries-related bacterial invasion. Abbreviations: CXCL10, CXC chemokine ligand 10; CXCR3, CXC chemokine receptor 3; IFN, interferon; FBS, fetal bovine serum; LTA, lipoteichoic acid; PGN, peptidoglycan; IL, interleukin; TNF, tumor necrosis factor; PBS, phosphate-buffered saline; ELISA, enzyme-linked immunosorbent assay; CCL, C-C chemokine ligand; TLR, Toll-like receptor; NOD, nucleotide oligomerization domain; HDPF, human dental pulp fibroblasts.
(キーワード): Adult / Bacteroides / Chemokine CXCL10 / Dental Caries / Dental Pulp / Fibroblasts / Humans / Immunohistochemistry / Lymphocyte Subsets / Middle Aged / RNA, Messenger / Receptors, CXCR3
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/154405910708601215
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18037659; ● Search Scopus @ Elsevier (PMID): 18037659; ● Search Scopus @ Elsevier (DOI): 10.1177/154405910708601215

(DOI: 10.1177/154405910708601215, PubMed: 18037659) Hiromichi Yumoto, Masayoshi Yamada, Chihiro Shinohara, Hideaki Nakae, Kanako Takahashi, Hiroyuki Azakami, Ebisu Shigeyuki and Takashi Matsuo :
Soluble products from Eikenella corrodens induce cell proliferation and expression of interleukin-8 and adhesion molecules in endothelial cells via mitogen-activated protein kinase pathways,
Oral Microbiology and Immunology, Vol.22, No.1, 36-45, 2007.

(要約): The periodontal vasculature is profoundly affected during the progression of periodontitis, and several specific bacteria are believed to be involved in this inflammatory disease. Eikenella corrodens is one of the common bacteria detected in periodontitis diseased lesions; however, the function of this organism in periodontitis is not well understood. In this study, we investigated the E. corrodens-induced endothelial cell alteration and inflammation process that leads to leukocyte infiltration in inflamed regions. Soluble products from E. corrodens (EcSP) induced the gene expression and protein production of vascular endothelial growth factor in oral epithelial cells and human umbilical vein endothelial cells (HUVEC). Direct stimulation by EcSP also activated endothelial cell proliferation. Moreover, EcSP induced ERK1/2 (p44/42) and p38 mitogen-activated protein kinase (MAPK) phosphorylation within 10-30 min in HUVEC, as demonstrated by Western blot analysis and up-regulated intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), E-selectin and interleukin-8 (IL-8) production demonstrated by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. The specific p38 MAPK inhibitor SB203580 reduced the expression of ICAM-1, VCAM-1 and IL-8, whereas the blockade of p44/42 by MAPK kinase (MEK1) inhibitor, PD98059, inhibited only IL-8 expression. Our results indicate that E. corrodens can trigger a cascade of events that induce inflammatory responses in periodontal tissue via the MAPK cascade and may promote chronic periodontitis without bacteria-cell contact.
(キーワード): Cell Adhesion Molecules / Cell Line / Cell Proliferation / Cells, Cultured / Culture Media, Conditioned / E-Selectin / Eikenella corrodens / Endothelial Cells / Endothelium, Vascular / Enzyme Inhibitors / Epithelial Cells / Flavonoids / Humans / Imidazoles / Intercellular Adhesion Molecule-1 / Interleukin-8 / KB Cells / Mitogen-Activated Protein Kinase 1 / Mitogen-Activated Protein Kinase 3 / Mitogen-Activated Protein Kinases / Periodontitis / Phosphorylation / Pyridines / Vascular Cell Adhesion Molecule-1 / Vascular Endothelial Growth Factor A / p38 Mitogen-Activated Protein Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1399-302X.2007.00320.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17241169; ● Search Scopus @ Elsevier (PMID): 17241169; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1399-302X.2007.00320.x

(DOI: 10.1111/j.1399-302X.2007.00320.x, PubMed: 17241169) Piotr Mydel, Yusuke Takahashi, Hiromichi Yumoto, Maryta Sztukowska, Malgorzata Kubica, Frank Gibson C. III, Donald Kurtz, James Travis, Vincent Collins, Ky-Anh Nguyen, Caroline A. Genco and Jan Potempa :
Roles of the host oxidative immune response and bacterial antioxidant Rubrerythrin during Porphyromonas gingivalis infection,
PLoS Pathogens, Vol.2, No.7, e76, 2006.

(要約): The efficient clearance of microbes by neutrophils requires the concerted action of reactive oxygen species and microbicidal components within leukocyte secretory granules. Rubrerythrin (Rbr) is a nonheme iron protein that protects many air-sensitive bacteria against oxidative stress. Using oxidative burst-knockout (NADPH oxidase-null) mice and an rbr gene knockout bacterial strain, we investigated the interplay between the phagocytic oxidative burst of the host and the oxidative stress response of the anaerobic periodontal pathogen Porphyromonas gingivalis. Rbr ensured the proliferation of P. gingivalis in mice that possessed a fully functional oxidative burst response, but not in NADPH oxidase-null mice. Furthermore, the in vivo protection afforded by Rbr was not associated with the oxidative burst responses of isolated neutrophils in vitro. Although the phagocyte-derived oxidative burst response was largely ineffective against P. gingivalis infection, the corresponding oxidative response to the Rbr-positive microbe contributed to host-induced pathology via potent mobilization and systemic activation of neutrophils. It appeared that Rbr also provided protection against reactive nitrogen species, thereby ensuring the survival of P. gingivalis in the infected host. The presence of the rbr gene in P. gingivalis also led to greater oral bone loss upon infection. Collectively, these results indicate that the host oxidative burst paradoxically enhances the survival of P. gingivalis by exacerbating local and systemic inflammation, thereby contributing to the morbidity and mortality associated with infection.
(キーワード): Animals / Antioxidants / Bacterial Proteins / Bacteroidaceae Infections / Disease Models, Animal / Female / Ferredoxins / Hemerythrin / Humans / Immunity, Mucosal / Mice / Mice, Inbred C57BL / Mice, Knockout / NADPH Oxidase / Neutrophils / Oxidative Stress / Porphyromonas gingivalis / Respiratory Burst / Rubredoxins
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.ppat.0020076
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16895445; ● Search Scopus @ Elsevier (PMID): 16895445; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.ppat.0020076

(DOI: 10.1371/journal.ppat.0020076, PubMed: 16895445) Yusuke Takahashi, Michael Davey, Hiromichi Yumoto, Frank Gibson C. III and Caroline Attardo Genco :
Fimbria-dependent activation of pro-inflammatory molecules in Porphyromonas gingivalis infected human aortic endothelial cells,
Cellular Microbiology, Vol.8, No.5, 738-757, 2006.

(要約): Epidemiological studies support that chronic periodontal infections are associated with an increased risk of cardiovascular disease. Previously, we reported that the periodontal pathogen Porphyromonas gingivalis accelerated atherosclerotic plaque formation in hyperlipidemic apoE-/- mice, while an isogenic fimbria-deficient (FimA-) mutant did not. In this study, we utilized 41 kDa (major) and 67 kDa (minor) fimbria mutants to demonstrate that major fimbria are required for efficient P. gingivalis invasion of human aortic endothelial cells (HAEC). Enzyme-linked immunosorbent assay (ELISA) revealed that only invasive P. gingivalis strains induced HAEC production of pro-inflammatory molecules interleukin (IL)-1beta, IL-8, monocyte chemoattractant protein (MCP)-1, intracellular adhesion molecule (ICAM)-1, vascular cellular adhesion molecule (VCAM)-1 and E-selectin. The purified native forms of major and minor fimbria induced chemokine and adhesion molecule expression similar to invasive P. gingivalis, but failed to elicit IL-1beta production. In addition, the major and minor fimbria-mediated production of MCP-1 and IL-8 was inhibited in a dose-dependent manner by P. gingivalis lipopolysaccharide (LPS). Both P. gingivalis LPS and heat-killed organisms failed to stimulate HAEC. Treatment of endothelial cells with cytochalasin D abolished the observed pro-inflammatory MCP-1 and IL-8 response to invasive P. gingivalis and both purified fimbria, but did not affect P. gingivalis induction of IL-1beta. These results suggest that major and minor fimbria elicit chemokine production in HAEC through actin cytoskeletal rearrangements; however, induction of IL-1beta appears to occur via a separate mechanism. Collectively, these data support that invasive P. gingivalis and fimbria stimulate endothelial cell activation, a necessary initial event in the development of atherogenesis.
(キーワード): Aorta / Bacteroidaceae Infections / Cells, Cultured / Chemokine CCL2 / E-Selectin / Endothelial Cells / Endothelium, Vascular / Enzyme-Linked Immunosorbent Assay / Fimbriae Proteins / Fimbriae, Bacterial / Humans / Intercellular Adhesion Molecule-1 / Interleukin-1 / Interleukin-8 / Mutation / Porphyromonas gingivalis / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1462-5822.2005.00661.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16611224; ● Search Scopus @ Elsevier (PMID): 16611224; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1462-5822.2005.00661.x

(DOI: 10.1111/j.1462-5822.2005.00661.x, PubMed: 16611224) Frank Gibson C. III, Hiromichi Yumoto, Yusuke Takahashi, Hsin-Hua Chou and Caroline Attardo Genco :
Innate immune signaling and Porphyromonas gingivalis-accelerated atherosclerosis,
Journal of Dental Research, Vol.85, No.2, 106-121, 2006.

(要約): Periodontal diseases are a group of diseases that lead to erosion of the hard and soft tissues of the periodontium, which, in severe cases, can result in tooth loss. Anecdotal clinical observations have suggested that poor oral health may be associated with poor systemic health; however, only recently have appropriate epidemiological studies been initiated, with defined clinical endpoints of periodontal disease, to address the association of periodontal disease with increased risk for cardiovascular and cerebrovascular disease. Although conflicting reports exist, these epidemiological studies support this connection. Paralleling these epidemiological studies, emerging basic scientific studies also support that infection may represent a risk factor for atherosclerosis. With P. gingivalis as a model pathogen, in vitro studies support that this organism can activate host innate immune responses associated with atherosclerosis, and in vivo studies demonstrate that this organism can accelerate atheroma deposition in animal models. In this review, we focus primarily on the basic scientific studies performed to date which support that infection with bacteria, most notably P. gingivalis, accelerates atherosclerosis. Furthermore, we attempt to bring together these studies to provide an up-to-date framework of emerging theories into the mechanisms underlying periodontal disease and increased risk for atherosclerosis, as well as identify intervention strategies to reduce the incidence of periodontal disease in humans, in an attempt to decrease risk for systemic complications of periodontal disease such as atherosclerotic cardiovascular disease.
(キーワード): Animals / Atherosclerosis / Disease Models, Animal / Endothelial Cells / Humans / Immunity, Innate / Inflammation Mediators / Macrophages / Mice / Periodontal Diseases / Porphyromonas gingivalis / Signal Transduction / Toll-Like Receptors
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/154405910608500202
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16434728; ● Search Scopus @ Elsevier (PMID): 16434728; ● Search Scopus @ Elsevier (DOI): 10.1177/154405910608500202

(DOI: 10.1177/154405910608500202, PubMed: 16434728) Takanari Miyamoto, Hiromichi Yumoto, Yusuke Takahashi, Michael Davey and Frank Gibson C. III :
Pathogen-accelerated atherosclerosis occurs early after exposure and can be prevented via immunization,
Infection and Immunity, Vol.74, No.2, 1376-1380, 2006.

(要約): Here we report on early inflammatory events associated with Porphyromonas gingivalis-accelerated atherosclerosis in apolipoprotein E knockout (ApoE-/-) mice. Animals challenged with P. gingivalis presented with increased macrophage infiltration, innate immune marker expression, and atheroma without elevated systemic inflammatory mediators. This early local inflammatory response was prevented in mice immunized with P. gingivalis. We conclude that localized up-regulation of innate immune markers early after infection, rather than systemic inflammation, contributes to pathogen-accelerated atherosclerosis.
(キーワード): Animals / Aorta, Thoracic / Apolipoproteins E / Atherosclerosis / Bacteroidaceae Infections / Immunity, Innate / Immunization / Inflammation / Male / Mice / Porphyromonas gingivalis / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.74.2.1376-1380.2006
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16428788; ● Search Scopus @ Elsevier (PMID): 16428788; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.74.2.1376-1380.2006

(DOI: 10.1128/IAI.74.2.1376-1380.2006, PubMed: 16428788) Hiromichi Yumoto, Hsin-Hua Chou, Yusuke Takahashi, Michael Davey, Frank Gibson C. III and Caroline A Genco :
Sensitization of human aortic endothelial cells to lipopolysaccharide via regulation of toll-like receptor 4 by bacterial fimbria-dependent invasion,
Infection and Immunity, Vol.73, No.12, 8050-8059, 2005.

(要約): Toll-like receptors (TLRs) are differentially up-regulated in response to microbial infection and chronic inflammatory diseases such as atherosclerosis. Epidemiological data support the idea that periodontal disease may be a risk factor for acceleration of atherosclerosis. Porphyromonas gingivalis, the etiological agent of periodontal disease, invades endothelium, has been detected in human atheromatous tissue, and accelerates atheroma formation in apolipoprotein E-/- mice with concurrent induction of TLRs in the aorta. As endothelial cells can present antigen via TLRs and play an important role in the development of atherosclerosis, we examined TLR expression in human aortic endothelial cells (HAEC) cultured with wild-type P. gingivalis, a fimbria-deficient mutant, and purified antigens. We observed increased TLR expression in HAEC infected with wild-type P. gingivalis by fluorescence-activated cell sorter, but not with noninvasive, fimbria-deficient mutant or purified P. gingivalis antigens. Following a wild-type P. gingivalis challenge, functional TLR2 and TLR4 activation was assessed by subsequent stimulation with TLR agonists Staphylococcus aureus lipoteichoic acid (SLTA; TLR2 ligand) and Escherichia coli lipopolysaccharide (LPS; TLR4 ligand). Unchallenged HAEC failed to elicit monocyte chemoattractant protein 1 (MCP-1) in response to LPS or SLTA but did so when cultured with wild-type P. gingivalis. P. gingivalis-induced TLR2 and -4 expression on HAEC functionally reacted to SLTA and E. coli LPS as measured by a further increase in MCP-1 production. Furthermore, MCP-1 expression elicited by E. coli LPS was inhibitable with TLR4-specific antibody and polymyxin B. These results indicate that invasive P. gingivalis stimulates TLR expression on the surface of endothelium and these primed cells respond to defined TLR-specific ligands.
(キーワード): Antigens, Bacterial / Aorta / Chemokine CCL2 / Cytochalasin D / Endothelial Cells / Endothelium, Vascular / Fimbriae, Bacterial / Humans / Ligands / Lipopolysaccharides / Porphyromonas gingivalis / Teichoic Acids / Toll-Like Receptor 4 / Toll-Like Receptors / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.73.12.8050-8059.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16299299; ● Search Scopus @ Elsevier (PMID): 16299299; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.73.12.8050-8059.2005

(DOI: 10.1128/IAI.73.12.8050-8059.2005, PubMed: 16299299) Hsin-Hua Chou, Hiromichi Yumoto, Michael Davey, Yusuke Takahashi, Takanari Miyamoto, Frank Gibson C. III and Caroline A. Genco :
Porphyromonas gingivalis Fimbria-Dependent Activation of Inflammatory Genes in Human Aortic Endothelial Cells,
Infection and Immunity, Vol.73, No.9, 5367-5378, 2005.

(要約): Epidemiological and pathological studies have suggested that infection with the oral pathogen Porphyromonas gingivalis can potentiate atherosclerosis and human coronary heart disease. Furthermore, infection with invasive, but not noninvasive P. gingivalis has been demonstrated to accelerate atherosclerosis in apolipoprotein E-deficient (ApoE(-/-)) mice and to accelerate local inflammatory responses in aortic tissue. In the present study, using high-density oligonucleotide microarrays, we have defined the gene expression profile of human aortic endothelial cells (HAEC) after infection with invasive and noninvasive P. gingivalis. After infection of HAEC with invasive P. gingivalis strain 381, we observed the upregulation of 68 genes. Genes coding for the cytokines Gro2 and Gro3; the adhesion molecules intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule (VCAM)-1, and ELAM-1 (E-selectin); the chemokine interleukin-8 (IL-8); and the proinflammatory molecules IL-6 and cyclooxygenase-2 were among the most highly upregulated genes in P. gingivalis 381-infected HAEC compared to uninfected HAEC control. Increased mRNA levels for signaling molecules, transcriptional regulators, and cell surface receptors were also observed. Of note, only 4 of these 68 genes were also upregulated in HAEC infected with the noninvasive P. gingivalis fimA mutant. Reverse transcription-PCR, enzyme-linked immunosorbent assay, and fluorescence-activated cell sorting analysis confirmed the expression of ICAM-1, VCAM-1, E-/P-selectins, IL-6, and IL-8 in HAEC infected with invasive P. gingivalis. We also demonstrated that increased expression of ICAM-1 and VCAM-1 in aortic tissue of ApoE(-/-) mice orally challenged with invasive P. gingivalis but not with the noninvasive P. gingivalis fimA mutant by immunohistochemical analysis. Taken together, these results demonstrate that P. gingivalis fimbria-mediated invasion upregulates inflammatory gene expression in HAEC and in aortic tissue and indicates that invasive P. gingivalis infection accelerates inflammatory responses directly in the aorta.
(キーワード): Aorta / Bacteroidaceae Infections / Cell Adhesion / Cytokines / Endothelium, Vascular / Fimbriae, Bacterial / Gene Expression Regulation / Immunity, Innate / Inflammation / Intercellular Adhesion Molecule-1 / Oligonucleotide Array Sequence Analysis / Porphyromonas gingivalis / Reverse Transcriptase Polymerase Chain Reaction / Up-Regulation / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.73.9.5367-5378.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16113252; ● Search Scopus @ Elsevier (PMID): 16113252; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.73.9.5367-5378.2005

(DOI: 10.1128/IAI.73.9.5367-5378.2005, PubMed: 16113252) Asaf Wilensky, Yankel Gabet, Hiromichi Yumoto, Yael Houri-Haddad and Lior Shapira :
Three-dimensional quantification of alveolar bone loss in Porphyromonas gingivalis-infected mice using micro-computed tomography,
Journal of Periodontology, Vol.76, No.8, 1282-1286, 2005.

(要約): Animal models are routinely used for the study of the pathogenesis of periodontal disease. However, some of the methods used for evaluating alveolar bone loss are limited to linear or two dimension measurements, while other methods, such as histology, are labor consuming. The present study was designed to evaluate a novel method for assessing the volume of alveolar bone loss in mice and to compare it to the traditional morphometric (linear) technique. Seven- to 8-week-old BALB/c mice were divided into three equal groups of six each; two groups were infected orally with Porphyromonas gingivalis (P. gingivalis) 381 or 53,977, while the third group was used as non-infected control. Forty-two days following infection, serum samples were obtained and maxillae were harvested. Bone loss was evaluated by micro-computed tomography (micro-CT) and by the morphometric technique. Significant decrease in residual supportive bone volumes (RSBV) was observed in both P. gingivalis-infected groups compared to the control group (P<0.0001). The P. gingivalis 53,977-infected group showed less residual supportive bone volume compared to the P. gingivalis 381-infected group, but there were no significant differences between these two groups. Using the morphometric technique, the differences between the three groups were not found to be statistically significant (P>0.05). The present study describes a novel micro-CT technique for volumetric evaluation of alveolar bone loss in mice. This technique is relatively simple and accurate, and due to its high sensitivity, enables the investigator to reduce the number of animals needed in each experimental group.
(キーワード): alveolar bone loss / models,animal / periodontal disease/diagnosis / Porphyromonas gingivalis / tomography,micro-computed
(出版サイトへのリンク): ● Publication site (DOI): 10.1902/jop.2005.76.8.1282
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16101359; ● Search Scopus @ Elsevier (PMID): 16101359; ● Search Scopus @ Elsevier (DOI): 10.1902/jop.2005.76.8.1282

(DOI: 10.1902/jop.2005.76.8.1282, PubMed: 16101359) Hiroyuki Azakami, Hiromi Akimichi, Masakatsu Usui, Hiromichi Yumoto, Shigeyuki Ebisu and Akio Kato :
Isolation and characterization of a plasmid DNA from periodontopathogenic bacterium, Eikenella corrodens 1073, which affects pilus formation and colony morphology,
Gene, Vol.351, 143-148, 2005.

(要約): Eikenella corrodens (Ec) is one of a group of periodontopathogenic bacteria. A plasmid DNA (8.7 kb) isolated from Ec 1073 was designated pMU1. Agarose gel electrophoresis and Southern analysis suggested that pMU1-like plasmids were carried in 2 Ec strains, including 1073, with higher hemagglutination (HA) activity than other strains. We determined the nucleotide sequence of this plasmid and identified 7 ORFs. A homology search revealed that 4 ORFs of pMU1 were homologous to ORFs in pJTPS1, found in a spontaneous avirulent mutant of the phytopathogenic bacterium, Ralstonia solanacearum. pJTPS1 is a putative hypovirulent plasmid, which is thought to control the virulence of R. solanacearum. We also found the ORF to be homologous to the recombinase specific to the type IV pilin gene. We introduced a part of pMU1 into the Ec 23834 strain, which has a pilus structure on its cell surface and forms corroding colonies on solid medium. No pilus structure was observed on the surface of transformants, most of which formed non-corroding colonies. When such transformants (or Ec 1073) were cured of pMU1 with acridine orange, they remained non-foliated and non-corroding. The results suggest that pMU1 might irreversibly affect pilus formation and colony morphology, and might be involved in the pathogenicity and virulence of Ec.
(キーワード): Pathogen / 歯周炎 (periodontal disease) / Phase variation / DNA sequencing
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.gene.2005.03.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15869847; ● Search Scopus @ Elsevier (PMID): 15869847; ● Search Scopus @ Elsevier (DOI): 10.1016/j.gene.2005.03.015

(DOI: 10.1016/j.gene.2005.03.015, PubMed: 15869847) Frank Gibson C. III, Charlie Hong, Hsin-Hua Chou, Hiromichi Yumoto, Jiqiu Chen, Egil Lien, Jodie Wong and Caroline Attardo Genco :
Innate Immune Recognition of Invasive Bacteria Accelerates Atherosclerosis in Apolipoprotein E-Deficient Mice,
Circulation, Vol.109, No.22, 2801-2806, 2004.

(要約): Infectious diseases have emerged as potential risk factors for cardiovascular disease (CVD). Epidemiological studies support a connection between periodontal disease, a chronic inflammatory disease of the supporting tissues of the teeth, and CVD. To directly test the connection between periodontal disease and atherosclerosis, apoE-/- mice were orally challenged with the periodontal disease pathogen Porphyromonas gingivalis or an invasion-impaired P gingivalis fimbriae-deficient mutant (FimA-). Both wild-type P gingivalis and the FimA- mutant were detected in blood and aortic arch tissue of apoE-/- mice by PCR after challenge. ApoE-/- mice challenged with wild-type P gingivalis presented with increased atherosclerotic plaque and expressed the innate immune response markers Toll-like receptor (TLR)-2 and TLR-4 in aortic tissue. Despite detection of the FimA- mutant in the blood and in aortic arch tissue, apoE-/- mice challenged with the FimA- mutant did not present with periodontal disease, upregulation of TLRs, or accelerated atherosclerosis. Furthermore, we demonstrate that immunization to control P gingivalis-elicited periodontal disease concomitantly prevents P gingivalis-accelerated atherosclerosis. We conclude that invasive P gingivalis accelerates atherosclerosis.
(キーワード): infection / inflammation / atherosclerosis / receptors / endothelium
(出版サイトへのリンク): ● Publication site (DOI): 10.1161/01.CIR.0000129769.17895.F0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15123526; ● CiNii @ 国立情報学研究所 (CRID): 1362825895621995520; ● Search Scopus @ Elsevier (PMID): 15123526; ● Search Scopus @ Elsevier (DOI): 10.1161/01.CIR.0000129769.17895.F0

(DOI: 10.1161/01.CIR.0000129769.17895.F0, PubMed: 15123526, CiNii: 1362825895621995520) Takashi Matsuo, Toshiyuki Shirakami, Kazumi Ozaki, Tadashi Nakanishi, Hiromichi Yumoto and Shigeyuki Ebisu :
An immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions,
Journal of Endodontics, Vol.29, No.3, 194-200, 2003.

(要約): We immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. Forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. The bacteria in the specimens were detected by Brown-Brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. Seventy percent of the examined teeth showed bacteria invading the dentinal tubules of the roots. Fusobacterium nucleatum, Eubacterium alactolyticum, E. nodatum, Lactobacillus casei, and Peptostreptococcus micros were abundant. Even in the canal-enlarged group, invasion of bacteria was observed in 65% of teeth. This study revealed the actual condition of bacteria in infected root dentin and suggested that the canal-enlargement procedure could not completely remove all the bacteria in the infected dentinal tubules of the root.
(キーワード): Adult / Aged / Bacteria / Coloring Agents / Dental Pulp / Dentin / Eubacterium / Fusobacterium nucleatum / Humans / Immune Sera / Immunoenzyme Techniques / Immunohistochemistry / Lactobacillus casei / Middle Aged / Peptostreptococcus / Periapical Diseases / Porphyromonas / Prevotella / Root Canal Preparation / Statistics, Nonparametric / Tooth Root
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00004770-200303000-00008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12669880; ● Search Scopus @ Elsevier (PMID): 12669880; ● Search Scopus @ Elsevier (DOI): 10.1097/00004770-200303000-00008

(DOI: 10.1097/00004770-200303000-00008, PubMed: 12669880) Masayoshi Yamada, Hideaki Nakae, Hiromichi Yumoto, Chihiro Shinohara, Shigeyuki Ebisu and Takashi Matsuo :
N-acetyl-D-galactosamine specific lectin of Eikenella corrodens induces intercellular adhesion molecule-1 (ICAM-1) production by human oral epithelial cells,
Journal of Medical Microbiology, Vol.51, No.12, 1080-1089, 2002.

(要約): During the acute inflammatory response in periodontitis, gingival epithelial cells are considered to play important roles in the recruitment of inflammatory cells to the site of infection through the secretion of chemokines. However, little is known about the expression of molecules that are involved in the interaction between the epithelium and neutrophils following bacterial attachment. Earlier work reported that periodontopathogenic Eikenella corrodens strain 1,073 up-regulated the expression and secretion of chemokines such as interleukin-8 (IL-8) from KB cells (a human oral epithelial cell line derived from a human oral epidermoid carcinoma). To elucidate the mechanism of the transmigration of neutrophils through the epithelium, the present study investigated the expression of adhesion molecules on KB cells in response to E. corrodens attachment. Adhesion molecule gene expression was assessed by RT-PCR and adhesion proteins expressed on KB cell surfaces were determined by cell-based ELISA and FACS. In RT-PCR, ICAM-1 mRNA levels were significantly increased within 1 h in response to exposure to E. corrodens and continued to increase over the 12-h period of study. In ELISA, increased surface ICAM-1 expression was paralleled by increased ICAM-1 mRNA levels. Furthermore, the increases in ICAM-1 expression on epithelial cells infected with E. corrodens were observed to be due to the N-acetyl-D-galactosamine (GalNAc) specific bacterial lectin-like substance of E. corrodens (EcLS), which was one of the adhesins of E. corrodens. This is the first study to report that a bacterial lectin-like substance increased the expression of ICAM-1 on gingival epithelial cells.
(キーワード): レクチン / ICAM-1 / Eikenella corrodens / 上皮細胞
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12466406; ● Search Scopus @ Elsevier (PMID): 12466406

(PubMed: 12466406) Yoshitaka Hosokawa, Tadashi Nakanishi, Daisuke Yamaguchi, Kanako Takahashi, Hiromichi Yumoto, Kazumi Ozaki and Takashi Matsuo :
Macrophage Inflammatory Protein 3α-CC chemokine receptor 6 interactions play an important role in CD4⁺ T-cell accumulation in periodontal diseased tissue.,
Clinical and Experimental Immunology, Vol.128, No.3, 548-554, 2002.

(要約): The regulatory role of chemokines and chemokine receptors on specific lymphocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that lymphocytes infiltrating inflamed gingival tissue expressed marked levels of CCR6. In periodontal diseased tissue, the expression of MIP-3alpha mRNA was detected by RT-PCR and further, MIP-3alpha was distributed in the basal layer of gingival epithelial cells, microvascular endothelial cells and the areas of inflammatory cells as shown by immunohistochemistry. Moreover, CCR6-expressing cells infiltrated into periodontal diseased tissue, and the proportion of CCR6-positive CD4+ T cells was significantly elevated in periodontal diseased tissue compared with peripheral blood in the same patients. Furthermore, gingival lymphocytes isolated from patients showed migration toward MIP-3alpha in an in vitro chemotaxis assay in which migration was abrogated by specific antibody to CCR6. Thus, these findings suggested that CCR6 and the corresponding chemokine, MIP-3alpha may have an important regulatory role in specific lymphocyte migration into inflamed periodontal tissue.
(キーワード): CCR6 / MIP-3 alpha / periodontal disease / MONOCYTE CHEMOATTRACTANT PROTEIN-1 / INFLAMED HUMAN GINGIVA / IMMUNOHISTOLOGICAL ANALYSIS / SELECTIVE RECRUITMENT / INDUCIBLE EXPRESSION / BACTERIA / IMMUNITY / HEALTHY / LESIONS / SUBSETS
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.1365-2249.2002.01865.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12067311; ● Search Scopus @ Elsevier (PMID): 12067311; ● Search Scopus @ Elsevier (DOI): 10.1046/j.1365-2249.2002.01865.x

(DOI: 10.1046/j.1365-2249.2002.01865.x, PubMed: 12067311) Hiromichi Yumoto, Hideaki Nakae, Masayoshi Yamada, Keiko Fujinaka, Chihiro Shinohara, Shigeyuki Ebisu and Takashi Matsuo :
Soluble products from Eikenella corrodens stimulate oral epithelial cells to induce inflammatory mediators,
Oral Microbiology and Immunology, Vol.16, No.5, 296-305, 2001.

(要約): In the inflammatory response elicited by bacterial colonization in periodontal pockets, pocket epithelial cells not only serve as a barrier to isolate the pocket microenvironment from external stimuli but also regulate the functions of neighboring cells including fibroblasts and inflammatory cells. To elucidate this mechanism, we characterized the effects of periodontopathic bacterium Eikenella corrodens 1073 components on the production of some inflammatory mediators in a human oral epithelial cell line (KB). In enzyme-linked immunosorbent assay (ELISA), the E. corrodens supernatant induced interleukin-6 (IL-6), IL-8 and prostaglandin E2 but not interferon-gamma (IFN-gamma) production by KB cells. After incubation with E. corrodens supernatant, KB cells showed a marked increase in the levels of IL-6, IL-8 and PG G/H synthase (cyclooxygenase)-2, but not IFN-gamma, gene expression by reverse-transcriptase polymerase chain reaction. All these E. corrodens products responsible for production of these inflammatory mediators resisted freezing and boiling and were present in a 10-kDa filtrate. These results imply that these soluble small-molecular-mass products from E. corrodens stimulate various inflammatory mediator productions by human oral epithelial cells and may play a role in the initiation of periodontal inflammation and subsequently perpetuate the inflammatory response during chronic infection.
(キーワード): Eikenella corrodens / 口腔上皮細胞 / プロスタグランディンE2 / インターロイキン6 / インターロイキン8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11555307; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034840691

(PubMed: 11555307, Elsevier: Scopus) 弘田克彦, 村田広美, 湯本浩通, 大倉一人, 長宗秀明, 三宅洋一郎 :
レンサ球菌のヒストン様蛋白質の構造解析,
Bacterial Adherence研究, Vol.14, 6-8, 2000年. Hiromichi Yumoto, Hideaki Nakae, Keiko Fujinaka, Shigeyuki Ebisu and Takashi Matsuo :
Interleukin-6 (IL-6) and IL-8 are induced in human oral epithelial cells in response to exposure to periodontopathic Eikenella corrodens,
Infection and Immunity, Vol.67, No.1, 384-394, 1999. 吉山昌宏, 浦山明久, 篠原千尋, 湯本浩通, 松尾敬志 :
X線微小分析法による象牙質接着界面の解析,
日本歯科保存学雑誌, Vol.41, No.2, 461-466, 1998年.

(キーワード): 象牙質 / レジン / XMA

Hiromichi Yumoto, Hiroyuki Azakami, Hideaki Nakae, Takashi Matsuo and Shigeyuki Ebisu :
Cloning, sequencing and expression of an Eikenella corrodens gene encoding a component protein of the lectin-like adhesin complex,
Gene, Vol.183, No.1-2, 115-121, 1996. Hiroyuki Azakami, Hiromichi Yumoto, Hideaki Nakae, Takashi Matsuo and Shigeyuki Ebisu :
Molecular analysis of the gene encoding a protein component of the Eikenella corrodens adhesin complex that is close to the carbohydrate recognition domain,
Gene, Vol.180, No.1-2, 207-212, 1996. Hideaki Nakae, Hiromichi Yumoto, Takashi Matsuo and Shigeyuki Ebisu :
Mitogenic stimulation of murine B lymphocytes by the N-acetyl-D-galactosamine specific bacterial lectin-like substance from Eikenella corrodens,
FEMS microbiology letters, Vol.116, No.3, 349-353, 1994.

MISC

松尾敬志, 中西正, 湯本浩通, 平尾功治, 篠原千尋, Toshihiko Tominaga :
感染根管治療の考え方,
Journal of Oral Health and Biosciences, Vol.28, No.2, 87-92, 2016年.

(要約): It is a well-established fact that bacterial infection of the dental pulp ultimately results in the formation of apical periodontitis. And apical periodontitis may develop periapical leisons consisting granulomas and cysts. In this article, we show the pathologic condition of infected root canal systems and periapical lesions immunohistologically. We also describe the possible causes of refractory apical periodontitis that conventional root canal therapy could not work effectively. Finally,we explain our newly developing treatment for refractory apical periodontitis named electro-magnetic apical treatment, and illustrate clinical efficacy of this treatment.
(キーワード): infected root canal treatment / refractory apical periodontitis / pathological condition
(徳島大学機関リポジトリ): ● Metadata: 109873
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050845762395157248

(徳島大学機関リポジトリ: 109873, CiNii: 1050845762395157248) 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 天羽崇, 三宅洋一郎 :
Streptococcus intermedius biofilm componentsの糖鎖結合能と炎症反応誘導能に関する解析,
Bacterial Adherence & Biofilm, Vol.29, 2016年. Park Hyuk Sang, Ye Ling, Love M. Robert, Farges Jean-Christophe and Hiromichi Yumoto :
Inflammation of the Dental Pulp,
Mediators of Inflammation, 980196, 2015.

(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2015/980196
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84956934621

(DOI: 10.1155/2015/980196, Elsevier: Scopus) 弘田克彦, 湯本浩通, 星野由美, 村上圭史, 宮本幸治, 松尾敬志, 三宅洋一郎 :
水溶性MPCポリマーの口腔細菌付着抑制効果,
Bacterial Adherence & Biofilm, Vol.24, 47-49, 2011年.

(要約): 口腔細菌によるbiofilm，すなわちdental plaqueの形成にはいくつかのkey stepがあり，dental plaque形成の抑制のためにはそれらのkey stepに効果的に働く方法を選択する必要が有る．我々は，超親水性表面を得ることができる2-methacryloloxethyl phosphorylcholine(MPC)co-polymerを用いてプラスチック表面をコートした界面は微生物の付着抑制に関与することを報告してきた．本実験では5%水溶性MPCポリマーで処理したHydroxyapatite(HY)プレートと培養歯肉上皮細胞(RT-7)へのStreptococcus mutansの付着状況を顕微鏡下にて観察した．その結果，5%水溶性MPCポリマー処理でHYプレート及び培養歯肉上皮細胞上へのS.mutansの付着は抑制されることが示された．以上よりHYプレートや培養歯肉上皮細胞に吸着した水溶性MPCポリマーは，プラスチックへのコーティングと同様にS.mutansの付着抑制効果を発揮することが示唆された．

石川清美, 渡邊久恵, 三木千津, 久保万友美, 山下美由紀, 大森幸子, 石川利栄子, 荒木哲也, 蓮井義則, 湯本浩通, 松尾敬志 :
歯周病患者に対する禁煙指導について, --- ニコチネルの応用 ---,
歯科衛生士, Vol.26, No.9, 77-81, 2002年. 三木千津, 石川清美, 渡邊久恵, 市原真由美, 久保万友美, 北林裕枝, 岡村由華, 細道美喜子, 石川利栄子, 綿城香, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
臼歯部の動揺を伴う歯周病患者への対応, --- 特にメインテナンスにおける歯科衛生士の役割 ---,
歯科衛生士, Vol.24, No.11, 81-85, 2000年. 石川清美, 渡邊久恵, 三木千津, 市原真由美, 久保万友美, 北林裕枝, 岡村由華, 細道美喜子, 石川利栄子, 綿城香, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
更年期障害を有する歯周病患者の一症例,
歯科衛生士, Vol.24, No.2, 81-84, 2000年. 渡邊久恵, 三好千奈, 三木千津, 石川清美, 池内明子, 蓮井義則, 石川利栄子, 綿城香, 石丸毅, 湯本浩通, 松尾敬志 :
口腔がん患者における歯周治療および管理の重要性,
歯科衛生士, Vol.23, No.9, 65-69, 1999年. 多田智代, 三好千奈, 渡邊久恵, 石川清美, 池内明子, 塚原ひとみ, 石川利栄子, 三森康智, 綿城香, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
喫煙習慣を持つ歯周病患者への対応,
歯科衛生士, Vol.22, No.8, 67-71, 1998年. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Biological Roles of Extracellular DNA in Bacterial Infection,
Asean Plus and Tokushima Joint International Conference, Program and Proceeding Book, 47-48, 2012. Katsuhiko Hirota, Takeyoshi Yoneyama, Mayumi Sakamoto, Hiroshi Miyamoto, Masanori Kurihara, Shizuo Kayama, Keiji Murakami, Hiromichi Yumoto, Takashi Matsuo and Yoichiro Miyake :
High prevalence of Pseudomonas aeruginosa from oropharyngeal biofilm in patients with cerebrovascular infarction and dysphagia.,
Chest, Vol.138, No.1, 237-238, 2010.

(キーワード): Aged / Biofilms / Cerebral Infarction / Deglutition Disorders / 女性 (female) / Humans / 男性 (male) / Oropharynx / Pneumonia, Aspiration / Prevalence / Pseudomonas Infections / 緑膿菌 (Pseudomonas aeruginosa)
(出版サイトへのリンク): ● Publication site (DOI): 10.1378/chest.10-0240
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20605833; ● Search Scopus @ Elsevier (PMID): 20605833; ● Search Scopus @ Elsevier (DOI): 10.1378/chest.10-0240

(DOI: 10.1378/chest.10-0240, PubMed: 20605833)

総説・解説

湯本浩通, 山村佳子, 石丸直澄, 宮本洋二 :
Dd診断力てすと歯肉と頬粘膜の白色病変,
デンタルダイアモンド, Vol.48, No.5, 25-26, 2023年4月. 湯本浩通, 青田桂子, 二宮雅美 :
歯肉頬移行部の腫瘤,
デンタルダイヤモンド, Vol.47, No.1, 25-26, 2022年1月.

(キーワード): 歯肉腫瘤 / 脂肪種

湯本浩通 :
歯内療法における技術革新と最近のトレンド・トピックス,
日本歯科保存学雑誌, Vol.64, No.3, 185-192, 2021年6月.

(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.64.185
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390288535621100032; ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.64.185

(DOI: 10.11471/shikahozon.64.185, CiNii: 1390288535621100032) 湯本浩通 :
歯内療法における技術革新,
デンタルダイヤモンド, Vol.46, No.681, 26-34, 2021年5月. 湯本浩通, 弘田克彦, 三宅洋一郎, 村上圭史, 藤原奈津美, 秋月皆人, 藤猪英樹 :
``撥菌''∼口腔バイオフィルム感染症予防への応用∼,
全国大学歯科衛生士教育協議会雑誌, Vol.10, No.25, 13-17, 2021年3月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521980706066501120

(CiNii: 1521980706066501120) 弘田克彦, 米山武義, 湯本浩通, 大野由香, 中石裕子, 野村加代, 坂本まゆみ, 和食沙紀, 濱田美晴, 内田智子 :
歯周病およびNETsとがんを含む全身疾患,
全国大学歯科衛生士教育協議会雑誌, Vol.10, No.25, 19-27, 2021年3月.

(キーワード): 歯周病 / 歯周病原細菌 / 感染症 / 非感染症 / NETs / がん / 転移 / 口腔健康管理
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523388080946625792

(CiNii: 1523388080946625792) 坂本英次郎, 廣島佑香, 木戸淳一, 西川泰史, 成石浩司, 木戸理恵, 湯本浩通 :
カルプロテクチンの歯周病病態における多様な役割と歯周病診断マーカーとしての可能性,
日本歯周病学会会誌, Vol.62, No.4, 193-199, 2020年12月.

(キーワード): カルプロテクチン / 抗菌ペプチド / 歯周病 / DAMPs / 歯周病診断マーカー
(出版サイトへのリンク): ● Publication site (DOI): 10.2329/perio.62.193
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1391975831222787968; ● Search Scopus @ Elsevier (DOI): 10.2329/perio.62.193

(DOI: 10.2329/perio.62.193, CiNii: 1391975831222787968) 湯本浩通, 平尾功治, 富永敏彦, 松尾敬志 :
高周波電流の根尖性歯周炎への応用,
歯内療法学会雑誌, Vol.41, No.2, 82-90, 2020年5月.

(徳島大学機関リポジトリ): ● Metadata: 115856

(徳島大学機関リポジトリ: 115856) 平尾功治, 二宮雅美, 湯本浩通 :
X線写真で認められた根尖部透過像,
デンタルダイヤモンド, Vol.45, No.662, 21-22, 2020年4月. 成石浩司, 湯本浩通 :
糖尿病と慢性腎疾患(CKD),
月刊糖尿病, Vol.11, No.4, 80-85, 2019年10月. 二宮雅美, 岩本勉, 湯本浩通 :
女児に認められた上顎前歯部の歯肉腫脹,
デンタルダイヤモンド, Vol.44, No.4, 15-16, 2019年3月.

(キーワード): 歯肉線維腫症

Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Daisuke Takegawa, Tadashi Nakanishi and Takashi Matsuo :
The Roles of Odontoblasts in Dental Pulp Innate Immunity,
Japanese Dental Science Review, Vol.54, No.3, 105-117, Aug. 2018.

(要約): Odontoblasts located in the outermost layer of dental pulp form a natural barrier between mineralized tissues, dentin, and soft tissues, dental pulp, of the vital tooth, and they first recognize caries-related pathogens and sense external irritations. Therefore, odontoblasts possess a specialized innate immune system to fight oral pathogens invading into dentin. Generally, the rapid initial sensing of microbial pathogens, especially pathogen-associated molecular patterns (PAMPs) shared by microorganisms, are mediated by pattern recognition receptors (PRRs), such as Toll-like receptor and the nucleotide-binding oligomerization domain (NOD). The innate immune responses in odontoblasts initiated by sensing oral pathogens provide host protective events, such as inflammatory reactions, to produce a variety of pro-inflammatory mediators, including chemokines and cytokines. These attract various inflammatory cells and cause antibacterial reactions, such as the production of defensins, to kill microorganisms in the proximal region of the odontoblast layer. This review focuses on innate immunity, especially cellular and molecular mechanisms regarding the sensing of PAMPs from oral pathogens by PRRs, in odontoblasts and provides information for future studies for the development of novel therapeutic strategies, including diagnosis and treatment, to prevent exceeding dental pulp inflammation and preserve the dental pulp tissues.
(徳島大学機関リポジトリ): ● Metadata: 112960
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jdsr.2018.03.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30128058; ● Summary page in Scopus @ Elsevier: 2-s2.0-85044379499

(徳島大学機関リポジトリ: 112960, DOI: 10.1016/j.jdsr.2018.03.001, PubMed: 30128058, Elsevier: Scopus) 湯本浩通 :
口腔Biofilm感染症における病原因子と全身に及ぼす影響,
Journal of Oral Health and Biosciences, Vol.31, No.1, 1-12, 2018年7月.

(要約): Oral infectious diseases, such as dental caries and periodontitis, are caused by oral biofilm, so-called dental plaque. The foundation of the biofilm structure is the extracellular polymeric substances consisting of polysaccharides, toxins, proteins and nucleic acids as well as vesicles, which secrete these components outside of bacterial cells. Among them, extracellular DNA (eDNA) has become increasingly recognized as a key component for biofilm formation and its structural stability. Bacterial histone-like DNA binding protein (HLP), an accessory architectural protein in a variety of bacterial cellular processes due to DNA and mRNA binding capacity, also contributes to the activation of host innate immunity during bacterial infection. Moreover, it has been suggested that vesicles harboring DNA are associated with horizontal gene transfer. In recent two decades, numerous studies regarding the association between oral biofilm infectious diseases and various systemic diseases, such as cardiovascular diseases, atherosclerosis, diabetes mellitus, aspiration pneumonia and autoimmune diseases, have been reported. Besides bacteria, Candida spp., which are frequently implicated in mixed bacterial–fungal infections in humans, can readily form biofilms on the surfaces of denture and cause a variety of infectious diseases ranging from denture stomatitis in denture wearers to life-threatening invasive infections, such as aspiration pneumonia, particularly in immunocompromised and elderly populations. From the viewpoint of biofilm infection, which cause systemic diseases as well as oral infectious diseases, the development of new preventive and therapeutic procedures targeting bacterial-derived pathogenic factors such as eDNA, HLP and vesicle, is expected in future studies.
(キーワード): 口腔Biofilm / 病原因子 / 菌体外DNA / DNA結合蛋白 / 全身疾患
(徳島大学機関リポジトリ): ● Metadata: 111931
(出版サイトへのリンク): ● Publication site (DOI): 10.20738/johb.31.1_1
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282763019594240; ● Search Scopus @ Elsevier (DOI): 10.20738/johb.31.1_1

(徳島大学機関リポジトリ: 111931, DOI: 10.20738/johb.31.1_1, CiNii: 1390282763019594240) 二宮雅美, 湯本浩通 :
歯根側面に認められたX線透過像,
デンタルダイヤモンド, Vol.43, No.4, 17-18, 2018年3月.

(キーワード): 垂直性歯根破折 / 画像診断 (diagnostic imaging)

松尾敬志, 尾崎和美, 中西正, 湯本浩通, 高橋加奈子, 平尾功治, 武川大輔 :
歯髄疾患の病態と診断,
日本歯内療法学会雑誌, Vol.34, No.2, 67-76, 2013年5月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680166022016

(CiNii: 1390282680166022016) 宮本貴成, 湯本浩通, Frank Gibson C. III :
心臓血管疾患と歯周病 –動脈硬化症への歯周病原性細菌感染のかかわり-,
ザ·クインテッセンス, Vol.25, No.5, 174-183, 2006年5月. 宮本貴成, 高橋祐介, 湯本浩通 :
抗生物質利用におけるEBM(根拠に基づく医学)を考える,
日本ヘルスケア歯科研究会誌, Vol.6, No.1, 17-22, 2004年. 湯本浩通, 松尾敬志, 市川哲雄 :
義歯とカンジダ,
医薬ジャーナル, Vol.37, No.10, 211-217, 2001年10月. Takashi Matsuo, Kazumi Ozaki, Hiromichi Yumoto and Masahiro Yoshiyama :
Caries-A disease of bacteria infection,
Caries Preventive restoration, 1-8, 2001. 中江英明, 湯本浩通, 山田真義, 篠原千尋, 松尾敬志 :
歯周炎発症における歯肉上皮細胞の役割,
四国歯学会雑誌, Vol.13, No.2, 259-265, 2001年.

(キーワード): 歯周病関連細菌 / 歯肉上皮細胞 / ケモカイン / 接着分子

阿座上弘行, 湯本浩通, 恵比須繁之 :
歯周病原性細菌は口腔内にどのように定着するか新たな診断·予防法の開発に向けて,
化学と生物, Vol.35, No.12, 848-853, 1997年12月.

講演・発表

Yumi Kuwamura, Kiyoe Kurahashi, Sumiko Yoshida, Hiromichi Yumoto, Maki Hosoki, Yukihiro Momota, Ken-ichi Aihara, Masuko Sumikawa, Hirokazu Uemura, Munehide Matsuhisa and Itsuro Endo :
Evaluation of Nursing Educational Material on Oral Health Behavior of Persons with Diabetes: Part 3 -- Physicians' Survey,
International Diabetes Federation Western Pacific Congress 2023 /15th Scientific Meeting of Asian Association for the study of Diabetes, Kyoto, Japan, Jul. 2023. Rika Saito, Masatake Akutagawa, Hiromichi Yumoto, Kouji Hirao, Takahiro Emoto, Hiroo Tarao, Toshihiko Tominaga, Toshitaka Ikehara, Emiko Yasuno and Yohsuke Kinouchi :
Relationship between current density and osteogenesis-promoting effect of osteoblasts,
THE 2nd Annual Conference of BioEM (BioEM 2023), Oxford, United Kingdom, Jun. 2023. Yusuke Kuroda, Masatake Akutagawa, Takahiro Emoto, Hiromichi Yumoto, Hiroo Tarao, Toshihiko Tominaga, Toshitaka Ikehara, Emiko Yasuno and Yohsuke Kinouchi :
A study of Coupled Thermal-Electrical Analysis for Lattice Network Model,
THE 2nd Annual Conference of BioEM (BioEM 2023), Oxford, United Kingdom, Jun. 2023. Raras Ajeng Enggardipta, Minato Akizuki, Kazumitsu Sekine, Kenichi Hamada, Akikazu Murakami and Hiromichi Yumoto :
The preliminary study of chitosan nanoparticles as antibacterial agent on Enterococcus faecalis biofilm,
19th Scientific Meeting and Refresher Course in Dentistry (Jakarta, Indonesia), Feb. 2023. Yumi Kuwamura, Maki Hosoki, Masuko Sumikawa, Yukihiro Momota and Hiromichi Yumoto :
Effectiveness of self-made video educational materials in a nursing skills class on oral care,
4th International Conference on Technology Competency as Caring in Nursing, 57, Web, Dec. 2022.

(キーワード): 口腔ケア (oral care) / nursing skill

Yusuke Kuroda, Masatake Akutagawa, Takahiro Emoto, Hiroo Tarao, Hiromichi Yumoto, Kouji Hirao, Tominaga Toshihiko, Toshitaka Ikehara, Emiko Yasuno and Yohsuke Kinouchi :
A study of electrode arrangement for electromagnetic wave therapy of periapical periodontitis using electro-thermal coupled analysis,
The 1st Annual Meeting of BioEM (BioEM 2022), Nagoya, Jun. 2022. Rika Saito, Masatake Akutagawa, Hiromichi Yumoto, Kouji Hirao, Takahiro Emoto, Hiroo Tarao, Tominaga Toshihiko, Toshitaka Ikehara, Emiko Yasuno and Yohsuke Kinouchi :
Effect of 500 kHz electric current application on osteoblastic bone regeneration,
The 1st Annual Meeting of BioEM (BioEM 2022), Nagoya, Jun. 2022. Makoto Funaki, Akiko Hata, Mitsuteru Yoshida, Naoki Miyamoto, Takahisa Ikuta, Yuta Uemura, MINATO AKIZUKI, Akira Tangoku, Hiromichi Yumoto, Hirohisa Ogawa and Takeshi Oya :
Quiescence Abrogates Stress-Induced Senescence-Associated Secretion Phenotype in Adipose-Derived Stem Cells and May Enhance Their Therapeutic Effects on Diabetic Complications,
Diabetes, Vol.71, No.Suppl_1, 305-OR, New Orleans, Jun. 2022. Futa Okido, Masatake Akutagawa, Hiromichi Yumoto, Takahiro Emoto, Hiroo Tarao, Toshihiko Tominaga, Kouji Hirao, Toshitaka Ikehara, Emiko Yasuno and Yohsuke Kinouchi :
Basic study on non-thermal effects in electro-sterilization using 500kHz burst current for Streptococcus mutans,
The Joint Annual Meeting of The Bioelectromagnetics Society and the European BioElectromagnetics Association (BioEM 2021), Ghent, Sep. 2021. Hiromichi Yumoto :
Clinical Technique in Endodontics,
ConsAsia2019 (The 1st General Meeting of Asian-Oceanian federation of Conservative Dentistry [AOFCD])(Seoul, Korea), Nov. 2019. Taketani Hideo, Masatake Akutagawa, Hiromichi Yumoto, Kouji Hirao, Takahiro Emoto, Tarao Hiroo, Tominaga Toshihiko, Toshitaka Ikehara and Yohsuke Kinouchi :
Experimental model simulation on electromagnetic wave therapy of apical periodontitis,
The Joint Annual Meeting of The Bioelectromagnetics Society and The European BioElectromagnetics Association (BioEM 2019) (Montpellier, France), Jun. 2019. Yasufumi Nishikawa, Koji Naruishi, Jun-ichi Kido and Hiromichi Yumoto :
Diabetic high-glucose condition enhances calprotectin-induced inflammatory responses in gingival fibroblasts,
97th General Session & Exhibition of the International Association for Dental Research (Vancouver, Canada), Jun. 2019. Yumi Kuwamura, Sumiko Yoshida, Kiyoe Kurahashi, Masuko Sumikawa, Eijiro Sakamoto, Akio Kuroda, Hikari Yamato, Misuzu Okamoto, Hirokazu Uemura, Daisuke Hinode, Hiromichi Yumoto, Ken-ichi Aihara, Makoto Funaki, Munehide Matsuhisa, Itsuro Endo and Sachi Kishida :
Oral health behaviors of outpatients with diabetes based on the Revised Diabetes Oral Health Assessment Tool for nurses,
Journal of Diabetes Investigation, Vol.10, No.Supplement1, 26, Sendai-City, Miyagi Prefecture, May 2019.

(キーワード): 口腔保健行動 (oral health behaviors) / diabetes nursing / アセスメント (assessment)

Yumi Kuwamura, Sumiko Yoshida, Kiyoe Kurahashi, Masuko Sumikawa, Eijiro Sakamoto, Ken-ichi Aihara, Hiromichi Yumoto, Munehide Matsuhisa, Itsuro Endo, Toshiyuki Yasui and Sachi Kishida :
The Relationship Between the Modified Diabetes Oral Health Assessment Tool© for Nurses, Self-Efficacy Scale, and Outcome Expectancy Scale for Self-Care of Patients with Periodontal Disease,
2nd Technological Competency as Caring in the Health Sciences 2018, 63, Aug. 2018.

(キーワード): nurse / 糖尿病 (diabetes mellitus) / oral health / self-efficacy / outcome expectancy

Hiromichi Yumoto :
The Influence of Oral Biofilm on Local Infection such as Periodontitis, and Systemic Diseases,
4th Dental Research, Exhibition, and Meeting (DREAM) (Yogyakarta, Indonesia), Aug. 2018. Eijiro Sakamoto, Yuji Inagaki, Ryosuke Takagi, Jun-ichi Kido and Hiromichi Yumoto :
AGEs and LPS Inhibit Osteoblastic Differentiation via Sclerostin in Osteocytes,
96th General Session & Exhibition of the International Association of Dental Research, Jul. 2018. Saori Matsui, Hazuki Maezono, Katsutaka Kuremoto, Naomichi Yoneda, Shigeyuki Ebisu, Hiromichi Yumoto, Kazunari Matoba, Takuya Ishimoto, Takayoshi Nakano, Yuichiro Noiri and Mikako Hayashi :
High-frequency Waves Promote Periapical Healing by Inducing Cell Growth Factors,
96th General Session & Exhibition of the International Association of Dental Research and Pan European Regional Congress (London, England), Jul. 2018. Yuji Inagaki, Kohei Nonaka, Ryosuke Takagi, Yasufumi Nishikawa, Rie Kido, Eijiro Sakamoto, Koji Naruishi, Jun-ichi Kido and Hiromichi Yumoto :
Kanroin Extracts Prevent Alveolar Bone Resorption in Experimental Rat Periodontitis,
96th General Session & Exhibition of the International Association of Dental Research, Jul. 2018. Natsumi Fujiwara, Hiromichi Yumoto, Keiji Murakami, Yasusei Kudo, Hiromi Nakae, Katsuhiko Hirota, Kazumi Ozaki and Yoichiro Miyake :
2-methacryloyloxyethyl-phosphorylcholine (MPC)-polymer suppresses the adherence of oral bacteria,
2018 IADR/PER General Session (ExCel London Convention Center, London, England) 25-28th July, 2018, Jul. 2018. Shunsuke Yamaguchi, Masatake Akutagawa, Hiromichi Yumoto, Kouji Hirao, Takahiro Emoto, Hiroo Tarao, Toshihiko Tominaga, Toshitaka Ikehara and Yohsuke Kinouchi :
Effect of high frequency electric field on growth factor of cultured osteoblasts,
2018 The Joint Annual Meeting of the Bioelectromagnetics Society and the European BioElectromagnetics Association, Piran, Jun. 2018.

(要約): 培養骨芽細胞に500kHz，20Vの正弦波高周波交流電圧を印加し，影響について調査した．温度，電圧，VEGF，VEGFmRNAおよびALPを測定した．電圧印加時間は30秒，60秒，90秒および120秒であった．その結果，骨芽細胞の骨形成に電気的要因が影響を与えることが示唆された．影響は細胞の初期状態に依存すると推定されるが，VEGFは電圧印加時間が90秒で有意に増加し，VEGF mRNAは120秒で有意に増加した．電圧印加によるALPの変化は今回の実験では観察されなかった．

Hiromichi Yumoto :
The Association Between Oral Bacterial Infections and Systemic Conditions, Especially Host Immune Responses,
The 4nd ASEAN Plus and Tokushima Joint International Conference on Challenge and Innovation in Oral Science (Sanur, Bali, Indonesia), Dec. 2017. Hiromichi Yumoto :
The use of 2-methacryloyloxyethyl phosphorylcholine polymer in prevention of periodontal diseases,
The 2nd International Conference on Health Sciences (Yogyakarta, Indonesia), Aug. 2017. Alma L. Jonarta, Hiromichi Yumoto, I. Astuti, W. Asmara and R. C. T. Tandelilin :
The effects of lipopolysaccharide-induced periodontal disease on the pulmonary lymphocytes of the rats model,
The 2nd International Conference on Health Sciences (Yogyakarta, Indonesia), Aug. 2017. Hiroaki Shuu, Masatake Akutagawa, Hiromichi Yumoto, Takahiro Emoto, Toshihiko Tominaga, Kouji Hirao, Hiroo Tarao, Toshitaka Ikehara and Yohsuke Kinouchi :
Fundamental study on the mechanism of electromagnetic wave therapy of periapical periodontitis,
2017 The Joint Annual Meeting of The Bioelectromagnetics Society (BEMS) and the European BioElectromagnetics Association (EBEA) (Hangzhou, China), Jun. 2017. Hiroo Tarao, Masatake Akutagawa, Takahiro Emoto, Hiromichi Yumoto, Toshihiko Tominaga, Takashi Matsumoto, Toshitaka Ikehara and Yohsuke Kinouchi :
Calculation of current densities inside dentin by applying 500-kHz current,
2017 The Joint Annual Meeting of The Bioelectromagnetics Society (BEMS) and the European BioElectromagnetics Association (EBEA) (Hangzhou, China), Jun. 2017. Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Katsuhiko Hirota, Yoichiro Miyake and Takashi Matsuo :
Streptococcal histone-like DNA binding protein induces MINCLE expression on monocytes,
95th General Session & Exhibition of the International Association for Dental Research, San Francisco, Mar. 2017. Yuki Hosokawa, Hiromichi Yumoto, Kouji Hirao, Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa and Takashi Matsuo :
Anti-inflammatory effects of polyphenols on rat odontoblastic cells,
95th General Session & Exhibition of the International Association for Dental Research (San Francisco), Mar. 2017. Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa and Takashi Matsuo :
Interferon-gamma modulates the innate immune response in odontoblast-like cells,
95th General session and exhibition of the IADR, Mar. 2017. Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa and Takashi Matsuo :
Effect of interleukin-17A on CCL20 production from odontoblast-like cells,
95th General session and exhibition of the IADR, Mar. 2017. Susilowati Heni, Keiji Murakami, Takashi Amoh, Katsuhiko Hirota, Yoichiro Miyake, Hiromichi Yumoto and Takashi Matsuo :
Royal Jelly inhibits Pseudomonas aeruginosa adherence and reduces excessive inflammatory resoponses in human epithelial cells,
2016 IADR, Seoul, Jun. 2016. Tarao Hiroo, Hiromichi Yumoto, Toshihiko Tominaga, Masatake Akutagawa, Takahiro Emoto, Toshitaka Ikehara and Yohsuke Kinouchi :
Current Distribution around Lesions due to Apical Periodontitis by Applying 500-kHz Current Across Electrodes,
2016 The Joint Annual Meeting of The Bioelectromagnetics and the European BioElectromagnetics Association (Ghent, Belgium), Jun. 2016. Toshihiko Tominaga, Kazuki Takahira, Hiromichi Yumoto, Takashi Matsuo, Naoki Bando and Kouji Hirao :
EMAT (Electromagnetic Apical Treatment): Clinical Application of Pulsed Electric Current Energization for Root Canal Treatment,
2015 Annual Session of the American Association of Endodontists, Seattle (USA), May 2015. Tadashi Nakanishi, Daisuke Takegawa, Hiromichi Yumoto, Kouji Hirao and Takashi Matsuo :
Interleukin-17 Regulates Inflammatory Mediator Expression in IL-1beta-stimulated Dental Pulp Cells,
93th General Session & Exhibition of the International Association for Dental Research, Boston (USA), Mar. 2015. Kouji Hirao, Hiromichi Yumoto, Chihiro Shinohara, Hosokawa Yuki and Takashi Matsuo :
Functional roles of NOD1 in odontoblasts on pulp innate immunity,
The 62nd annual meeting of japanese association for dental research, 99, Dec. 2014. Hiromichi Yumoto, Takashi Matsuo, Katsuhiko Hirota, Kouji Hirao, Miyazaki Tsuyoshi, Keiji Murakami, Natsumi Fujiwara and Yoichiro Miyake :
Anti-inflammatory and protective effects of 2-methacryloyloxyethyl-phosphorylcholine-polymer on oral epithelial cells,
92th General Session & Exhibition of the International Association for Dental Research (Cape Town, South Africa), Jun. 2014. Nur Ashikin, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, 天羽崇 and Yoichiro Miyake :
Extracellular DNA affects Streptococcus mutans biofilm through quorum sensing system and histone-like DNA binding protein expression,
The 12th Japan-Korea International Symposium on Microbiology, Tokyo, Mar. 2014. Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Keiji Murakami, Yoichiro Miyake and Takashi Matsuo :
Pathogenic roles of Streptococcal histone-like protein in microbial infection,
The 15th Joint Scientific Meeting of JSCD-KACD (Gyeongju, Korea), Nov. 2013. Toshihiko Tominaga, Naoki Bando, Hiromichi Yumoto and Takashi Matsuo :
Trial of Electro-Magnetic Wave Irradiation Therapy for Vertical Root Fracture as Tooth Conservative Therapy: a Case report,
The 9th World Endodontic Congress, Tokyo, Japan, May 2013. Naoki Bando, Toshihiko Tominaga, Hiromichi Yumoto and Takashi Matsuo :
Clinical Application of Electro-Magnetic Wave Irradiation to the Treatment for Periapical Periodontitis; Case Report,
The 9th World Endodontic Congress, Tokyo, Japan, May 2013. Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Prostaglandin F2a Regulates Cytokine Expression in Dental Pulp Cells,
91th General Session & Exhibition of the IADR, Seattle, USA, Mar. 2013. Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Interferon-g modulates the innate immune response of dental pulp cells,
91th General Session & Exhibition of the IADR, Seattle, USA, Mar. 2013. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Biological Roles of Extracellular DNA in Bacterial Infection,
Asean Plus and Tokushima Joint International Conference, Yogyakarta, Dec. 2012. Hiromichi Yumoto, Toshihiko Tominaga, Kouji Hirao, Naoki Bando, Takashi Sumitomo, Kanako Takahashi and Takashi Matsuo :
Signaling Pathways and Gene Expression Activated by Electro-Magnetic-Wave in Osteoblastic-Cells,
90th General Session and Exhibition of the IADR, Iguazu Falls, Brazil, Jun. 2012. Kouji Hirao, Hiromichi Yumoto, Katsuhiko Hirota, Dali Liu, Nur Asikin, Kanako Takahashi, Takashi Matsuo and Yoichiro Miyake :
Global Gene Analysis in Monocytes Stimulated with Streptococcal Histone-Like Protein,
90th General Session and Exhibition of the IADR, Iguazu Falls, Brazil, Jun. 2012. Toshihiko Tominaga, Hiromichi Yumoto and Takashi Matsuo :
Clinical Application of Electro-Magnetic Wave Irradiation for Infected Root Canal Treatment -EMAT (Electro-Magnetic Apical Treatment) -,
2012 Annual Session of the American Association of Endodontists, Boston, USA, Apr. 2012. Hiromichi Yumoto, Toshihiko Tominaga and Takashi Matsuo :
Electro-Magnetic Wave Irradiation Stimulates Osteoblasts to Enhance Cell Growth and Gene Expression of Growth Factors,
2012 Annual Session of the American Association of Endodontists, Boston, USA, Apr. 2012. Hiromichi Yumoto :
Basic Research aimed at Application of Electro-Magnetic Wave Irradiation for Endodontic Treatment,
13th Joint-Scientific Meeting of KACD and JSCD (Seoul, Korea), Nov. 2011. Liu Dali, Li Yunpeng, Zhou Yanbin, Naito Mariko, Hiromichi Yumoto, Yoichiro Miyake, Liang Jingping and Shu Rong :
Genome Comparison of A Clinical Virulent Porphyromonas gingivalis Strain With Type Strains W83 And ATCC33277,
International Union of Microbiological Societies 2011 Congress, Sep. 2011. Katsuhiko Hirota, Hiromichi Yumoto, Nur Asikin, Kouji Hirao, Kanako Takahashi, Liu Dali, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Gene Expression Profiling of Human Monocytic THP-1 Cells Stimulated With Extracellular Streptococcus intermedius Histone-Like DNA Binding Protein,
International Union of Microbiological Societies 2011 Congress, Sep. 2011. Takashi Sumitomo, Toshihiko Sumitomo, Naoki Bando, Saki Hirao, Shinya Kako, Hiromichi Yumoto and Takashi Matsuo :
A Case Report; Electro-Magnetic Apical Treatment,
The 9th KAE-JEA Joint Meeting, Jul. 2011. Yoichiro Miyake, Katsuhiko Hirota, Hiromichi Yumoto, N Yamamoto, K Miyamoto and Takashi Matsuo :
Inhibition of the adherence of oral bacterial by MPC-polymer,
EUROBIOFILMS 2011 -Second European Congress on Microbial Biofilms-Basic and Dlinical Aspedts-, Copenhagen, Jul. 2011. Tsuyoshi Fujita, Hiromichi Yumoto, Kazuhisa Ouhara, Hideki Shiba, Tsuyoshi Miyagawa, S Matsuda, Hiroyuki Kawaguchi, Takashi Matsuo and Hidemi Kurihara :
Irsogladine Maleate Regulates Gingival Epithelial Barrier Functions In Inflammatory Condition,
89th General Session & Exhibition of the International Association for Dental Research, Mar. 2011. Hiromichi Yumoto :
The Relationship between Periodontitis and Cardiovascular Disease,
International Joint Symposium on Oral Science, Featuring: Oral Health-related Improvements Promote Quality of Life, Dec. 2010. Tadashi Nakanishi, Kouji Hirao, Takegawa Daisuke, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Catechins suppress cyclooxygenase-2 expression in human dental pulp cells,
88th General Session and Exhibition of the IADR, Jul. 2010. Toshihiko Tominaga, Hiromichi Yumoto and Takashi Matsuo :
Novel Therapeutic Approach of Electro-Magnetic Wave for Infected Root Canal,
88th General Session and Exhibition of the IADR, Jul. 2010. Takegawa Daisuke, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Interferon-gamma enhances toll-like receptor ligand-induced cytokine production in pulpal cells,
88th General Session and Exhibition of the IADR, Jul. 2010. Hiromichi Yumoto, T. TOMINAGA, Kouji Hirao, Tomoko Kimura and Takashi Matsuo :
Inactivation and Bactericidal Activity against Oral Bacteria of Electro-Magnetic Wave,
88th General Session and Exhibition of the IADR, Jul. 2010. Liu Dali, Hiromichi Yumoto, Liang Ping Jing, Yoichiro Miyake and Shu Rong :
Recombinant Porphyromonas gingivalis DnaK purification and Pg-dnaK mutant creation,
2nd Meeting of IADR Pan Asian Pacific Federation and the 1st Meeting of IADR Asia/Pacific Region, Sep. 2009. Toshihiko Tominaga, Takashi Sumitomo, Hiromichi Yumoto and Takashi Matsuo :
The effects of Electro-Magnetic-Wave on bacterial viability and osteoblastic cell proliferation,
American Association of Endodontics, 2009 Annual Session, Apr. 2009. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Pro-inflammatory Roles of NOD2 in Human Gingival Fibroblasts,
The 3rd International Symposium on "Oral Sciences to Improve the Quality of Life", Tokushima, Sep. 2008. Hiromichi Yumoto, Liu Dali, Keiji Murakami, Katsuhiko Hirota, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
Gene-regulatory and Physiological Functions of Histone-like Protein from Streptococci,
86th General Session and Exhibition of the International Association for Dental Research, Toronto (Canada), Jul. 2008. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Pro-inflammatory Roles of NOD2 in Human Gingival Fibroblasts,
86th General Session and Exhibition of the International Association for Dental Research, Toronto, Jul. 2008. Kayo Mukai, Tadashi Nakanishi, Hiromichi Yumoto, Kanako Takahashi, Kouji Hirao and Takashi Matsuo :
Effects of Catechins on Cytokine Production in Human Odontoblast-like Cells,
86th General Session and Exhibition of the International Association for Dental Research, Jul. 2008. Kouji Hirao, Hiromichi Yumoto, Tadashi Nakanishi, Kanako Takahashi, Kayo Mukai, Miho Yoneda and Takashi Matsuo :
Functional Analysis of Pattern Recognition Receptor Expression in Pulpal Cells,
86th General Session and Exhibition of the International Association for Dental Research, Jul. 2008. Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
The essentiality and involvements of streptocuccus intermedius histone-like protein in bacterial viability and normal growth,
The 2nd International Symposium on "The Future Direction of Oral Sciences in the 21st Century", Tokushima, Dec. 2007. Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi, Kayo Mukai and Takashi Matsuo :
Expression and Functional Analyses of Pattern recognition Receptor (PRR) in Cultured Human Dental Pulp Cells,
The 1st international symposium and workshop "The Future Direction of Oral Sciences in the 21st century", Mar. 2007. Liu Dali, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake :
Investigation of the toxicity of Streptococcus intermedius histone-like protein in Escherichia coli,
The 1st international symposium and workshop ''The Future Direction of Oral Sciences in the 21st century'', Awajishima, Mar. 2007. Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi, Kayo Mukai and Takashi Matsuo :
Expression and Functional Analyses of Pattern recognition Receptor (PRR) in Cultured Human Dental Pulp Cells,
The 8th Joint-Scientific Meeting between KACD & JSCD, Busan(Korea), Nov. 2006. Miho Yoneda, Hideaki Nakae, Hiromichi Yumoto, Chihiro Shinohara and Takashi Matsuo :
Expression pattern of claudin family in human gingival tissues,
84th General Session and Exhibition of the International Association for Dental Research, Jun. 2006. Xinyan Liu, Takashi Ukai, Hiromichi Yumoto, Michael Davey, Frank Gibson C. III and Caroline A. Genco :
TLR2 plays a critical role in the atherosclerotic inflammatory response in ApoE-deficient mice independent of dietary lipids,
Toll2006 meeting. Recent Advances in Pattern Recognition, Salvador(Brazil), Mar. 2006. Caroline A. Genco, Takanari Miyamoto, Hiromichi Yumoto, Sophia Ostrer-Potapov, Michael Davey, Yusuke Takahashi and Frank Gibson C. III :
Acute and Early Recognition of Invasive Bacteria Accelerates Atherosclerosis in Apolipoprotein E-deficient Mice,
42nd Annual Meeting of Infectious Diseases Society of America, Boston(USA), Sep. 2004. Asaf Wilensky, Yael Houri-Haddad, A. Halabi, Hiromichi Yumoto, Caroline A. Genco and Lior Shapira :
P.gingivalis-Induced Bone Loss by Different Strains Correlates with Antibody Levels,
2004: Joint Meeting CED, Israeli Division, NOF; Joint Meeting of the Continental European (CED), Israeli and Scandinavian (NOF) Divisions of IADR, Istanbul(Turkey), Aug. 2004. Hsin-Hua Chou, Hiromichi Yumoto, Michael Davey, Yusuke Takahashi, Frank Gibson C. III and Caroline A. Genco :
Identification of human genes differentially regulated by invasive and non-invasive P. gingivalis infection of human aortic endothelial cells,
第19回生物医学総合学術年会, 台北(台湾), Apr. 2004. Hiromichi Yumoto, Hsin-Hua Chou, Yusuke Takahashi, Michael Davey, Frank Gibson C. III and Caroline A. Genco :
Invasive Porphyromonas gingivalis up-regulate Toll-like receptors in endothelial cells/monocytes,
82th General Session and Exhibition of the International Association for Dental Research, Honolulu(USA), Mar. 2004. Hsin-Hua Chou, Hiromichi Yumoto, Michael Davey, Yusuke Takahashi, Frank Gibson C. III and Caroline A. Genco :
Human genes regulated by invasive and non-invasive Porphyromonas gingivalis,
82th General Session and Exhibition of the International Association for Dental Research, Honolulu(USA), Mar. 2004. Yusuke Takahashi, Hiromichi Yumoto, Piotr Mydel, Michael Davey, Maryta Sztukowska, James Travis, Jan Potempa, Frank Gibson C. III and Caroline A. Genco :
Porphyromonas gingivalis gingipain mutants are attenuated in a mouse model,
82th General Session and Exhibition of the International Association for Dental Research, Honolulu(USA), Mar. 2004. Piotr Mydel, Yusuke Takahashi, Hiromichi Yumoto, Maryta Sztukowska, Donald Kurtz, James Travis, Jan Potempa and Caroline A. Genco :
Oxygen-Burst strategy is ineffective against Porphyromonas gingivalis protected by rubrerythrin,
82th General Session and Exhibition of the International Association for Dental Research, Honolulu(USA), Mar. 2004. Yusuke Takahashi, Hiromichi Yumoto, Michael Davey, Frank Gibson C. III and Caroline A. Genco :
Atherogenic responses in endothelial cells following infection with Porphyromonas gingivalis,
82th General Session and Exhibition of the International Association for Dental Research, Honolulu(USA), Mar. 2004. Frank Gibson C. III, Charlie Hong, Jodie Wong, Hiromichi Yumoto and Caroline A. Genco :
Immunization Prevents Infection-Induced Acceleration of Atherosclerosis in ApoE-Deficient Mice,
Scientific Sessions 2003, American Heart Association, Orlando(USA), Nov. 2003. Frank Gibson C. III, Charlie Hong, Jodie Wong, Hiromichi Yumoto and Caroline A. Genco :
Oral Infection with Invasive Porphyromonas gingivalis Stimulates Accelerated Atherosclerotic Plaque Accumulation in ApoE-/- Mice,
42nd InterScience Conference on Antimicrobial Agents and Chemotherapy, San Diego(USA), Sep. 2002. Hiromichi Yumoto, Katsuhiko Hirota, Hideaki Nagamune, Kazuto Ohkura, Keiji Murakami, Masayoshi Yamada, Hideaki Nakae, Yoichiro Miyake and Takashi Matsuo :
Molecular analysis of histone-like protein from Streptococcus intermedius,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Masayoshi Yamada, Hiromichi Yumoto, Chihiro Shinohara, Hideaki Nakae, Shigeyuki Ebisu and Takashi Matsuo :
Eikenella corrodens Induces Endothelial Cells via MAPK Family pathways,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Naoki Ohmura, Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, Hideo Kanitani, Toshiya Kashiwabara, Yoichiro Miyake and Tetsuo Ichikawa :
Influence of denture plaque on the pharyngeal microflora,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Yoshitaka Hosokawa, Tadashi Nakanishi, Daisuke Yamaguchi, Hiromichi Yumoto and Takashi Matsuo :
LARC-CCR6 Interactions play an important role in CD4+ T cell accumulation in periodontal diseased tissue,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Tadashi Nakanishi, Kanako Takahashi, Yoshitaka Hosokawa, Daisuke Yamaguchi, Chihiro Shinohara, Hiromichi Yumoto and Takashi Matsuo :
Expression of macrophage inflammatory protein-3 α in human pulpitis,
80th General SEssion ofthe IADR(International Association of Dental Research), San Diego(USA), Mar. 2002. Hideaki Nakae, Masayoshi Yamada, Hiromichi Yumoto, Chihiro Shinohara and Takashi Matsuo :
Bacterial lectin of Eikenella corrodens induces cytokines and adhesion molecules,
Modern Periodontology New Directions in 21st Century-, Kanagawa(Japan), Jun. 2001. Hiromichi Yumoto, Daisuke Yamaguchi, Yoshitaka Hosokawa, Chihiro Shinohara, Tadashi Nakanishi, Hirotoshi Shimizu, Masayoshi Yamada, Hideaki Nakae and Takashi Matsuo :
Expression of IL-18 and IL-18 receptor in human periodontally diseased tissue,
Modern Periodontology New Directions in 21st Century-, Kanagawa(Japan), Jun. 2001. Chihiro Shinohara, Hiromichi Yumoto, Masayoshi Yamada, Hideaki Nakae, Shigeyuki Ebisu and Takashi Matsuo :
Secreted Products from Eikenella corrodens Activate Endothelial cells.,
The 86th Annual Meeting of the American Academy of Periodontology inconjunction with the Japanese Society of Periodontology, Hawaii(USA), Sep. 2000. Masayoshi Yamada, Hiromichi Yumoto, Hideaki Nakae, Chihiro Shinohara, Ebisu Shigeyuki and Takashi Matsuo :
Activation of mitogen-activated protein kinases in oral epithelial cells by Secreted Eikenella corrodens,
The 86th Annual Meeting of the American Academy of Periodontology inconjunction with the Japanese Society of Periodontology, Hawaii(USA), Sep. 2000. Hiromichi Yumoto, Hideaki Nakae, Masayoshi Yamada, Shigeyuki Ebisu and Takashi Matsuo :
Eikenella corrodens induces prostaglandin E2 production and cyclooxygenase-2 expression in oral epithelial cells,
11th International Conference on Periodontal Research, Goteborg(Sweden), Jun. 1999. Hideaki Nakae, Masayoshi Yamada, Hiromichi Yumoto, Shigeyuki Ebisu and Takashi Matsuo :
Infection of human oral epithelial cells with Eikenella corrodens upregulates ICAM-1 expression,
11th International Conference on Periodontal Research, Goteborg(Sweden), Jun. 1999. 西川泰史, 川野弘道, 友竹偉則, 湯本浩通, 市川哲雄 :
高グルコース培養したヒト歯肉線維芽細胞に Candidalysin誘導性ROSがもたらす影響について,
四国歯学会第63回例会, 2024年3月. 二宮雅美, 植村勇太, 湯本浩通 :
歯周歯内病変による重度骨欠損部位に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行った一症例,
特定非営利活動法人日本歯科保存学会2023年度秋季学術大会(第159回)プログラムおよび講演抄録集, 152, 2023年11月.

(キーワード): 歯周歯内病変 / 歯周組織再生療法 / リグロス / サイトランスグラニュール

植村勇太, 二宮雅美, 植村友美, 湯本浩通 :
歯根短小を伴う広汎型侵襲性歯周炎(StageⅣ Grade C)に対して包括的歯周治療を行った一症例,
第66回秋季日本歯周病学会学術大会プログラムおよび演題抄録集, 178, 2023年10月.

(キーワード): 侵襲性歯周炎 / 歯周組織再生療法 / 歯根短小

桑村由美, 瀧川稲子, 湯本浩通, 細木眞紀, 桃田幸弘, 澄川真珠子, 倉橋清衛, 上村浩一 :
糖尿病患者への口腔保健行動の看護支援教材の作成に向けてー第4報看護師を対象とした調査,
第28回日本糖尿病教育・看護学会学術集会, Vol.27, 149, 2023年9月. 植村友美, 植村勇太, 二宮雅美, 湯本浩通 :
長期喫煙歴のある広汎型慢性歯周炎患者に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行った症例,
日本歯周病学会中国四国3大学・日本臨床歯周病学会中国四国支部合同研修会, 2023年9月.

(キーワード): 喫煙 (smoking) / 歯周組織再生療法 / リグロス / サイトランスグラニュール

松岡敦嗣, 芥川正武, 木内陽介, 榎本崇宏, 湯本浩通, 秋月皆人, 富永敏彦, 太良尾浩生, 池原敏孝, 安野恵実子 :
温度時間積からみたStreptococcus mutansの電気殺菌における非熱的効果について,
令和5年度電気・電子・情報関係学会四国支部連合大会講演論文集, 198, 2023年9月. 黒田悠介, 芥川正武, 太良尾浩生, 榎本崇宏, 湯本浩通, 富永敏彦, 池原敏孝, 安野恵実子, 木内陽介 :
電熱連成解析を用いた高周波根管治療における後継永久歯への影響に関する検討,
令和5年度電気・電子・情報関係学会四国支部連合大会講演論文集, 197, 2023年9月. 桑村由美, 細木眞紀, 澄川真珠子, 湯本浩通, 桃田幸弘, 上村浩一 :
看護大学生の口腔保健行動の一年間の縦断調査:オリジナル動画やデンタルシミュレーターを講義・演習に取入れて,
第267回徳島医学会学術集会(令和5年度夏期), 27, 2023年8月. 西川泰史, 川野弘道, 友竹偉則, 湯本浩通, 市川哲雄 :
ヒト歯肉線維芽細胞におけるCandidalysin誘導性ROSの産生性とその作用について,
四国歯学会第62回例会, 2023年7月. 二宮雅美, 生田貴久, 秋月皆人, 植村勇太, 湯本浩通 :
PCRを用いたTreponeme denticola, Tannerella forsythia迅速検出システムの有用性,
日本歯科保存学会 2023年度春季学術大会(第158回)プログラムおよび講演抄録集, 142, 2023年6月.

(キーワード): 歯周病原細菌 / Point of Care Testing / Porphyromonas gingivalis / Treponeme denticola / Tannerella forsythia

松田真司, 菅谷勉, 加藤幸紀, 根本英二, 竹内康雄, 山下慶子, 沼部幸博, 西田哲也, 小方頼昌, 申基喆, 長野孝俊, 両角俊哉, 小松康高, 出分菜々衣, 後藤亮真, 北村正博, 田口洋一郎, 高柴正悟, 湯本浩通, 山下明子, 吉永泰周, 吉村篤利, 河口浩之 :
全身性疾患への影響を考慮した新たな歯周病重症度検査項目の策定∼学会主導型多施設臨床研究∼ 第二報,
第66回春季日本歯周病学会学術大会(高松), 2023年5月. 湯本浩通 :
PCRを用いた歯周病原細菌迅速検出システムの有用性,
第66回春季日本歯周病学会学術大会(高松), 2023年5月. 植村勇太, 二宮雅美, 植村友美, 湯本浩通 :
喫煙歴のある広汎型重度慢性歯周炎患者に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行った一症例,
第66回春季日本歯周病学会学術大会, Vol.65, No.April, 165, 2023年5月.

(キーワード): 歯周組織再生療法 / リグロス / サイトランスグラニュール / 喫煙 (smoking)

坂東美和, 二宮雅美, 安田勝裕, 湯本浩通 :
下顎右側第一大臼歯部の根分岐部病変に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行った一症例,
第66回春季日本歯周病学会学術大会, Vol.65, No.April, 165, 2023年5月.

(キーワード): 歯周組織再生療法 / リグロス / サイトランスグラニュール / 根分岐部病変

武川香織, 藤田晶帆, 福本仁美, 鶴田真生, 清野方子, 西川泰史, 川野弘道, 友竹偉則, 湯本浩通 :
高齢者における各種歯ブラシの歯垢除去効果について,
第66回春季日本歯周病学会学術大会(高松), 2023年5月. 西川泰史, 植村勇太, 二宮雅美, 湯本浩通 :
遊離歯肉移植術によりインプラント周囲粘膜の清掃性の改善を図った一症例,
第66回春季日本歯周病学会学術大会, 2023年5月. 枡富健二, 板東美香, 稲垣裕司, 木戸理恵, 植村勇太, 畑田祐佳里, 木戸淳一, 湯本浩通 :
糖尿病関連歯周炎高齢患者のオーラルフレイルリスクバイオマーカーの探索,
第66回春季日本歯周病学会学術大会(高松), 2023年5月. 木戸理恵, 廣島佑香, 木戸淳一, 吉田賀弥, 生田貴久, 湯本浩通 :
人工合成したSecretory leukocyte protease inhibitorの抗菌活性とリポソーム封入,
第66回春季日本歯周病学会学術大会, 2023年5月. 船木真理, 秦明子, 吉田光輝, 宮本直輝, 生田貴久, 植村勇太, 秋月皆人, 丹黒章, 湯本浩通, 小川博久, 尾矢剛志 :
休眠状態の脂肪組織由来間葉系幹細胞による細胞老化随伴分泌現象の解除と糖尿病性合併症治療への応用の可能性,
糖尿病, Vol.66, No.Suppl.1, S.246, 2023年5月. 桑村由美, 湯本浩通, 細木眞紀, 桃田幸弘, 澄川真珠子, 倉橋清衛, 吉田守美子 :
糖尿病がある人の口腔保健行動のチームでの支援にむけて第2報ブラッシングの継続支援の工夫に焦点をあてた報告,
第66回日本糖尿病学会年次学術集会, Vol.66, No.Supplement, S-316, 2023年5月. 西川泰史, 川野弘道, 友竹偉則, 湯本浩通, 市川哲雄 :
高グルコース負荷を行ったヒト歯肉線維芽細胞における Candidalysinの作用,
四国歯学会第 61回例会, 2023年3月. 西松佳苗, 清水日向, 中村大輔, 植村勇太, 稲垣裕司, 廣島佑香, 木戸淳一, 村上明一, 湯本浩通 :
Porphyromonas gingivalis由来OMVsは破骨細胞の分化と歯槽骨吸収に関与する,
四国歯学会第61回例会, 2023年3月. 西川泰史, 湯本浩通 :
予後不良と診断を受けた歯に対して歯根尖切除術を行った3症例,
日本歯科保存学会2022年度秋季学術大会(第157回), 2022年11月. 仲村大輔, 植村勇太, 廣島佑香, 村上圭史, 村上明一, 湯本浩通 :
Porphyromonas gingivalis 由来OMVsは破骨細胞の分化と歯槽骨吸収に関与する,
第75回日本細菌学会中国・四国支部総会(岡山・WEB), 2022年10月. 湯本浩通 :
ポストコロナ時代の口腔健康管理―口腔感染症と全身疾患の関連性―,
日本歯科衛生学会第17回学術大会(徳島・WEB), 2022年9月. 福井かおり, 松村佑季, 溝江里美, 弘田克彦, 吉田幸子, 湯本浩通, 岡本好史 :
歯周病関連細菌の過剰な菌体外DNA結合タンパク質が宿主免疫機構に与える影響,
日本歯科衛生学会第17回学術大会(徳島・WEB), 2022年9月. 桑村由美, 湯本浩通, 細木眞紀, 桃田幸弘, 澄川真珠子, 吉田守美子, 倉橋清衛, 瀧川稲子 :
糖尿病をもつ人の口腔保健行動のチームでの支援にむけて第1報歯科医師による口腔保健行動の支援と看護師への期待,
第27回日本糖尿病教育・看護学会学術集会, Vol.26, 145, 2022年9月.

(キーワード): 糖尿病 (diabetes mellitus) / 口腔保健行動 (oral health behaviors) / 看護師

湯本浩通, 二宮雅美, 木戸淳一 :
骨格性不正咬合による矯正治療患者に対して包括的歯周治療を行った一症例,
第65回秋季日本歯周病学会学術大会(仙台), 2022年9月. 稲垣裕司, 板東美香, 生田貴久, 木戸淳一, 湯本浩通 :
侵襲性歯周炎(ステージIII, グレードC)に対して歯周基本治療と歯周外科治療を行い奏功した一症例,
第65回秋季日本歯周病学会学術大会(仙台), 2022年9月. 板東美香, 稲垣裕司, 湯本浩通 :
歯肉退縮に対して根面被覆を行った2症例∼成功症例と失敗症例の考察∼,
第65回秋季日本歯周病学会学術大会, 2022年9月. 松田真司, 菅谷勉, 加藤幸紀, 根本英二, 竹内康雄, 喜田大智, 沼部幸博, 西田哲也, 小方頼昌, 申基喆, 長野孝俊, 両角俊哉, 小松康高, 出分菜々衣, 神谷洋介, 北村正博, 田口洋一郎, 高柴正悟, 湯本浩通, 山下明子, 吉永泰周, 吉村篤利, 河口浩之 :
全身疾患への影響を考慮した新たな歯周病重症度検査項目の策定,
第65回秋季日本歯周病学会学術大会(仙台), 2022年9月. 湯本浩通 :
歯周病と象牙質知覚過敏症との関連,
第65回秋季日本歯周病学会学術大会(仙台), 2022年9月. 植村勇太, 稲垣裕司, 廣島佑香, 仲村大輔, 木戸淳一, 湯本浩通 :
Porphyromonas gingivalis由来OMVs (Outer Membrane Vesicles)は破骨細胞の分化に関与する,
第65回秋季日本歯周病学会学術大会, 2022年9月. 西川泰史, 川野弘道, 友竹偉則, 湯本浩通, 市川哲雄 :
Candidalysinがインプラント周囲炎の病態形成に与える影響について,
四国歯学会第60回例会, 2022年6月. 木戸淳一, 廣島佑香, 木戸理恵, 吉田賀弥, 稲垣裕司, 成石浩司, 湯本浩通 :
人工合成したβ-defensin 2によるPorphyromonas gingivalisの付着抑制およびリポソーム封入と口腔上皮細胞への送達,
四国歯学会第60回例会, 2022年6月. 西川泰史, 植村勇太, 川野弘道, 友竹偉則, 二宮雅美, 湯本浩通 :
インプラント周囲粘膜に対して歯周形成手術を行いデジタル画像解析により粘膜厚径評価を行った一症例,
第65回春季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.64, 150, 2022年6月.

(キーワード): インプラント (implant) / 歯周形成外科 / デジタル画像解析

和田明大, 二宮雅美, 金崎伸幸, 植村勇太, 湯本浩通 :
垂直的骨欠損に人工骨とリグロスを併用した歯周組織再生療法を行った後，人工骨の分離が生じた1症例,
第65回春季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.64, 146, 2022年6月.

(キーワード): 歯周組織再生療法 / 線維芽細胞増殖因子2 / 歯周病 (periodontitis) / リグロス

木戸淳一, 廣島佑香, 木戸理恵, 吉田賀弥, 稲垣裕司, 成石浩司, 湯本浩通 :
人工合成したbeta-defensin 2によるPorphyromonas gingivalisの付着抑制およびリポソーム封入と口腔上皮細胞への送達,
第65回春季日本歯周病学会学術大会, 2022年6月. 船木真理, 吉田光輝, 宮本直輝, 生田貴久, 植村勇太, 秋月皆人, 秦明子, 丹黒章, 湯本浩通, 小川博久, 尾矢剛志 :
休眠状態の導入・維持による脂肪組織由来間葉系幹細胞の若返りを通じた，糖尿病性合併症治療への応用の可能性,
糖尿病, Vol.65, No.Suppl.1, S257, 2022年5月. 桑村由美, 澄川真珠子, 湯本浩通, 細木眞紀, 桃田幸弘, 吉田守美子, 倉橋清衛, 上村浩一 :
コロナ禍で実施された糖尿病患者への教育・指導の方法についての文献による検討,
第65回日本糖尿病学会年次学術集会, Vol.65, No.Supplement, S233, 2022年5月. 木戸理恵, 坂本英次郎, 廣島佑香, 板東美香, 木戸淳一, 生田貴久, 二宮雅美, 稲垣裕司, 西川泰史, 友竹偉則, 成石浩司, 湯本浩通 :
イムノクロマト法を用いたカルプロテクチン測定による歯周病およびインプラント疾患の診断,
四国歯学会第59回例会, 2022年3月. 湯本浩通, 平尾功治, 富永敏彦, 多田瑛一朗, 芥川正武, 榎本崇宏, 太良尾浩生, 池原敏孝, 安野恵実子, 木内陽介 :
高周波電流通電・電磁波照射の歯根膜細胞に対する影響と難治性根尖性歯周炎に対する臨床応用,
LED総合フォーラム2022 in 徳島, 179-180, 2022年1月. 大城戸楓太, 芥川正武, 湯本浩通, 榎本崇宏, 太良尾浩生, 富永敏彦, 平尾功治, 池原敏孝, 安野恵実子, 木内陽介 :
500 kHzの高周波電圧印加による電気殺菌の非熱的効果の基礎的研究,
LED総合フォーラム2022 in 徳島, 141-142, 2022年1月. 湯本浩通 :
歯肉上皮の役割・機能を再考する-歯周病の発症からバイオマーカーの探索・予防まで-,
日本歯周病学会中国四国3大学・日本臨床歯周病学会中国四国支部合同研修会, 2021年11月. 西川泰史, 二宮雅美, 川野弘道, 友竹偉則, 湯本浩通 :
限局型侵襲性歯周炎患者に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行い1年半経過した1症例,
日本歯周病学会中国四国3大学・日本臨床歯周病学会中国四国支部合同研修会, 2021年11月.

(キーワード): 侵襲性歯周炎 / リグロス / サイトランスグラニュール

植村勇太, 廣島佑香, 村上圭史, 稲垣裕司, 藤猪英樹, 湯本浩通 :
Porphyromonas gingivalis OMVによる歯肉上皮細胞のサイトカイン産生機構,
四国歯学会第58回例会, 2021年11月. 西川泰史, 成石浩司, 二宮雅美, 植村勇太, 湯本浩通 :
2次性咬合性外傷を有する広汎型重度慢性歯周炎患者に包括的歯周治療を行った一症例,
日本歯科保存学会2021年度秋季学術大会(第155回)プログラムおよび講演抄録集, 2021年10月.

(キーワード): 侵襲性歯周炎

湯本浩通, 二宮雅美 :
遺伝性疾患(ファブリー病)を有する慢性歯周炎患者に対して矯正治療と歯周外科治療を行った一症例,
第64回秋季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.63, 154, 2021年10月.

(キーワード): 遺伝性疾患 / 歯周組織再生 / 小矯正

二宮雅美, 植村勇太, 秋月皆人, 生田貴久, 湯本浩通 :
感冒後に重篤化した壊死性潰瘍性歯肉炎の一症例,
第64回秋季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.63, 161, 2021年10月.

(キーワード): 壊死性潰瘍性歯肉炎 / 免疫機能低下 / 感冒 / 接触障害

湯本浩通, 二宮雅美, 木戸淳一 :
遺伝性疾患(ファブリー病)を有する慢性歯周炎患者に対して矯正治療と歯周外科治療を行った一症例,
第64回秋季日本歯周病学会学術大会, 2021年10月. 廣島佑香, 木戸理恵, 木戸淳一, 稲垣裕司, 成石浩司, 湯本浩通 :
人工合成したLopocalin 2はPorphyromonas gingivalisの口腔上皮細胞への付着を抑制する,
第64回秋季日本歯周病学会学術大会, 2021年10月. 秋月皆人, 植村勇太, 村上圭史, 廣島佑香, 藤猪英樹, 湯本浩通 :
歯周病原細菌とその線毛TypeのPCRによる迅速検出システムの有効性の検討,
第74回日本細菌学会中国・四国支部総会, 2021年10月. 植村勇太, 廣島佑香, 村上圭史, 多田彩乃, 桑原知巳, 藤猪英樹, 湯本浩通 :
Porphyromonas gingivalis OMVによる歯肉上皮細胞のサイトカイン産生機構,
第74回日本細菌学会中国・四国支部総会, 2021年10月. 植村勇太, 廣島佑香, 村上圭史, 稲垣裕司, 湯本浩通, 藤猪英樹 :
ヒト歯肉上皮細胞におけるPorphyromonas gingivalis由来メンブレンベシクルの炎症性サイトカイン産生誘導機構の解明,
第19回四国免疫フォーラム, 2021年6月. 大城戸楓太, 芥川正武, 湯本浩通, 榎本崇宏, 太良尾浩生, 富永敏彦, 平尾功治, 池原敏孝, 安野恵実子, 木内陽介 :
細菌細胞懸濁液への均一電流印加のためのTrench 型電圧印加装置の製作,
第60回日本生体医工学会大会, 2021年6月. 桑村由美, 澄川真珠子, 細木眞紀, 湯本浩通, 桃田幸弘, 吉田守美子, 倉橋清衛, 上村浩一 :
糖尿病患者の口腔保健行動の看護支援のための教育教材の開発に向けてー第1報糖尿病患者の義歯に関する文献検討,
糖尿病, Vol.64, No.Supplement, 2021年5月.

(キーワード): 糖尿病 (diabetes mellitus) / 口腔保健行動 (oral health behaviors) / 看護 (nursing)

武川香織, 湊晶帆, 長岡仁美, 鶴田真生, 清野方子, 西川泰史, 川野弘道, 友竹偉則, 湯本浩通 :
高齢者の口腔衛生管理における幅広植毛歯ブラシの有用性について,
第64回春季日本歯周病学会学術大会, 2021年5月. 木戸淳一, 廣島佑香, 木戸理恵, 吉田賀弥, 稲垣裕司, 成石浩司, 湯本浩通 :
リポソームに封入したリポカリン2の口腔上皮細胞への送達,
第64回春季日本歯周病学会学術大会, 2021年5月. 西川泰史, 二宮雅美, 川野弘道, 友竹偉則, 湯本浩通 :
限局型侵襲性歯周炎患者に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法とインプラント治療を行った一症例,
第64回春季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.63, 119, 2021年5月.

(キーワード): 歯周組織再生療法 / リグロス / サイトランスグラニュール / 歯周病 (periodontitis)

生田貴久, 二宮雅美, 秋月皆人, 植村勇太, 成石浩司, 湯本浩通 :
PCRを用いたPorphyromonas gingivalis2型線毛迅速検出システムの有効性の検討,
第64回春季日本歯周病学会学術大会プログラムおよび演題抄録集, Vol.63, 24, 2021年5月.

(キーワード): PCR / Porphyromonas gingivalis

廣島佑香, 植村勇太, 稲垣裕司, 木戸淳一, 湯本浩通 :
ヒト歯肉上皮細胞におけるPorphyromonas gingivalis由来メンブレンベシクルの炎症性サイトカイン産生誘導機構の解明,
第64回春季日本歯周病学会学術大会, 2021年5月. 秋月皆人, 二宮雅美, 生田貴久, 植村勇太, 湯本浩通 :
チェアサイドでのPorphyromonas gingivalis迅速検出システムの有用性,
四国歯学会第57回例会, 2021年3月.

(キーワード): PCR検査 / 歯周病原細菌 / Porphyromonas gingivalis

湯本浩通 :
歯内療法における技術革新と最近のトレンド・トピックス,
日本歯科保存学会2020年度秋季学術大会(第153回), 2020年11月. 植村勇太, 廣島佑香, 稲垣裕司, 湯本浩通 :
Porphyromonas gingivalis由来メンブレンベシクルがヒト歯肉上皮細胞に及ぼす影響,
日本歯科保存学会2020年度秋季学術大会(第153回), 2020年11月. 秋月皆人, 二宮雅美, 植村勇太, 生田貴久, 成石浩司, 湯本浩通 :
PCRを用いたPorphyromonas gingivalis迅速検出システムの有用性,
日本歯科保存学会第153回 2020年度秋季学術大会, 2020年11月.

(キーワード): 歯周病 (periodontitis) / Porphyromonas gingivalis / PCR / 臨床検査 (clinical laboratory testing)

植村勇太, 廣島佑香, 村上圭史, 多田彩乃, 桑原知巳, 藤猪英樹, 湯本浩通 :
Porphyromonas gingivalis由来メンブレンベシクルがヒト歯肉上皮細胞に及ぼす影響,
第73回日本細菌学会中国・四国支部総会, 32, 2020年10月. 秋月皆人, 村上圭史, 廣島佑香, 藤猪英樹, 湯本浩通 :
MPCポリマーコーティングにおけるTi表面への歯周病原菌付着抑制効果の検討,
第73回日本細菌学会中国・四国支部総会, 30, 2020年10月. 木戸理恵, 木戸淳一, 坂本英次郎, 西川泰史, 廣島佑香, 湯本浩通 :
イムノクロマト法を用いたカルプロテクチンの測定によるインプラント疾患の診断,
第63回秋季日本歯周病学会学術大会, Vol.62, 123, 2020年10月. 二宮雅美, 植村勇太, 和田明大, 生田貴久, 湯本浩通 :
下顎第一大臼歯の重度骨欠損に対してリグロスとサイトランスグラニュールを併用した歯周組織再生療法を行った一症例,
日本歯周病学会会誌, Vol.62, 134, 2020年10月.

(キーワード): 歯周組織再生療法 / 重度歯周炎 / リグロス / サイトランスグラニュール

竹谷英雄, 芥川正武, 太良尾浩生, 榎本崇宏, 湯本浩通, 平尾功治, 富永敏彦, 池原敏孝, 安野恵実子, 木内陽介 :
高周波電圧印加による根管治療時の至適条件探索を目的とした電熱連成解析システムの開発,
2020年電子情報通信学会ソサイエティ大会講演論文集, 324, 2020年9月. 木戸淳一, 廣島佑香, 木戸理恵, 稲垣裕司, 成石浩司, 湯本浩通 :
無細胞蛋白質合成系を用いた抗菌ペプチドの合成とリポソーム封入,
日本歯科保存学会2020年度春季学術大会(第152回)(神戸), 2020年6月. 廣島佑香, 木戸淳一, 木戸理恵, 吉田賀弥, 稲垣裕司, 成石浩司, 湯本浩通 :
オーラルケアへの応用に関連するリポソームのデリバリー法の検討,
第63回春季日本歯周病学会学術大会, 2020年5月. 木戸理恵, 廣島佑香, 生田貴久, 稲垣裕司, 板東美香, 成石浩司, 木戸淳一, 湯本浩通 :
最終糖化産物による口腔上皮細胞の Lipocalin2 発現誘導は好中球の遊走性とサイトカイン発現を調節する,
第63回春季日本歯周病学会学術大会, 2020年5月. 植村勇太, 二宮雅美, 和田明大, 木戸理恵, 高木亮輔, 野中康平, 谷口裕哉, 生田貴久, 坂本英次郎, 板東美香, 稲垣裕司, 成石浩司, 木戸淳一, 湯本浩通 :
歯周病ならびに歯周組織再生療法に関する臨床研究について,
徳島県歯科医学大会・四国歯学会第56回例会, 2020年2月. 高木亮輔, 二宮雅美, 植村勇太, 湯本浩通 :
中等度慢性歯周炎患者の幅広の骨内欠損に対しリグロスと骨補填材を併用した再生療法を行った一症例,
日本歯周病学会中国四国3大学・日本臨床歯周病学会中国四国支部合同研修会, 2019年12月.

(キーワード): 歯周病 (periodontitis) / 歯周組織再生療法 / リグロス

二宮雅美, 生田貴久, 成石浩司, 湯本浩通 :
重度慢性歯周炎を有する血液透析患者に対して包括的歯周治療を行った一症例,
日本歯科保存学会 2019年度秋季学術大会(第151回), 2019年11月.

(キーワード): 歯周炎 (periodontal disease) / 透析 (dialysis) / 歯周治療 / ペリオドンタルメディシン

蔵本瞳, 平尾功治, 細川由樹, 武川大輔, 湯本浩通, 中西正 :
ラット象牙芽細胞(KN-3)におけるVEGFの石灰化誘導作用,
日本歯科保存学会 2019年度秋季学術大会(第151回) (福岡), 2019年11月. 野中康平, 板東美香, 木戸淳一, 稲垣裕司, 坂本英次郎, 成石浩司, 湯本浩通 :
ショウガオールはヒト歯肉線維芽細胞においてAGEs誘導性の酸化ストレスを抑制する,
第62回秋季日本歯周病学会学術大会 (北九州), 2019年10月. 廣島佑香, 木戸淳一, 木戸理恵, 吉田賀弥, 稲垣裕司, 成石浩司, 湯本浩通 :
オーラルケアへの応用を目指した抗菌ペプチド合成とリポソーム封入,
第62回秋季日本歯周病学会学術大会, 2019年10月. 神尾強司, 守田剛, 角田佳折, 湯本浩通, 馬場麻人 :
萌出後マウス臼歯の歯髄における象牙質形成関連因子の発現解析,
第61回歯科基礎医学会学術大会, 2019年10月.

(キーワード): 歯髄 (pulp) / 象牙質 / in situ hybridization

山口舜祐, 芥川正武, 湯本浩通, 平尾功治, 榎本崇宏, 太良尾浩生, 富永敏彦, 池原敏孝, 木内陽介 :
高周波交流電圧印加による骨芽細胞への影響,
令和元年度電気関係学会四国支部連合大会講演論文集, 25, 2019年9月. 平尾功治, 湯本浩通, 富永敏彦, 太良尾浩生, 榎本崇宏, 芥川正武, 池原敏孝, 木内陽介 :
高周波電流を用いた歯周組織再生療法,
生体医工学シンポジウム2019 (徳島), 2019年9月. 高木亮輔, 坂本英次郎, 稲垣裕司, 成石浩司, 木戸淳一, 湯本浩通 :
S100A9はMAPKおよびSTAT3経路を介して骨細胞におけるIL-6とRANKLの発現を調節する,
日本歯科保存学会 2019年度春季学術大会(第150回) (金沢), 2019年6月. 稲垣裕司, 生田貴久, 坂本英次郎, 木戸淳一, 湯本浩通 :
全顎的歯周治療に伴って関節リウマチの状態が改善した一症例,
日本歯科保存学会2019年度春季学術大会(第150回), 2019年6月. 武川大輔, 中西正, 平尾功治, 湯本浩通, 細川由樹, 蔵本瞳, 松尾敬志 :
NODリガンド刺激したヒト象牙芽細胞様細胞におけるインターフェロンγの影響,
第150回日本歯科保存学会 2019年度春季学術大会, 2019年6月. 蔵本瞳, 湯本浩通, 平尾功治, 細川由樹, 松尾敬志 :
Caffeic Acid Phenetyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生誘導機序の解析,
第40回日本歯内療法学会学術大会 (東京), 2019年6月. 大石慶二, 富田秀伸, 湯本浩通 :
上顎右側犬歯の歯頚部外部吸収に対して外科的治療を行った一症例,
第40回日本歯内療法学会学術大会, 2019年6月. 金崎伸幸, 二宮雅美, 湯本浩通 :
矯正治療中に進行した侵襲性歯周炎患者に対して歯周組織再生療法を行った一症例,
日本歯周病学会会誌, Vol.61, 149, 2019年5月.

(キーワード): 侵襲性歯周炎 / 歯周組織再生療法 / 患者教育

二宮雅美, 坂本英次郎, 成石浩司, 生田貴久, 高木亮輔, 畑中加珠, 岡本憲太郎, 小野晋太郎, 高柴正悟, 湯本浩通 :
周期性好中球減少症を有する母娘に認められた重度歯周炎の症例,
日本歯周病学会会誌, Vol.61, 164, 2019年5月.

(キーワード): 周期性好中球減少症 / 家族性 / 重度歯周炎

木戸理恵, 廣島佑香, 生田貴久, 吉田賀弥, 稲垣裕司, 成石浩司, 尾崎和美, 木戸淳一, 湯本浩通 :
最終糖化産物はヒト口腔上皮細胞のリポカリン2発現を増加する,
第62回春季日本歯周病学会学術大会, 2019年5月. 松井沙織, 前園葉月, 呉本勝隆, 米田直道, 恵比須繁之, 湯本浩通, 的場一成, 石本卓也, 中野貴由, 野杁由一郎, 林美加子 :
ラット根管治療モデルを用いた高周波根尖療法の評価,
第149回日本歯科保存学会秋季学術大会(京都), 2018年11月. 稲垣裕司, 高木亮輔, 西川泰史, 木戸理恵, 坂本英次郎, 成石浩司, 木戸淳一, 湯本浩通 :
甘露飲エキスは破骨細胞と骨芽細胞の分化制御を介してラット実験的歯周炎の歯槽骨吸収を抑制する,
日本歯科保存学会2018年度秋季学術大会(第149回), 2018年11月. 二宮雅美, 生田貴久, 成石浩司, 湯本浩通 :
口腔内細菌が原因で発症した感染性心内膜炎患者に対して包括的歯周治療を行った一症例,
日本歯科保存学会2018年度秋季学術大会(第149回), 163, 2018年11月.

(キーワード): 感染性心内膜炎 / 口腔内球菌 / 歯周治療

美原(和田) 智恵, 木戸淳一, 二宮雅美, 湯本浩通 :
薬物性歯肉増殖を伴う高齢の慢性歯周炎患者に対して包括的歯周治療を行った1症例,
第62回秋季日本歯周病学会学術大会, 2018年10月. 美原(和田) 智恵, 木戸淳一, 二宮雅美, 湯本浩通 :
薬物性歯肉増殖を伴う高齢の慢性歯周炎患者に対して包括的歯周治療を行った一症例,
第61回秋季日本歯周病学会学術大会, Vol.60, 153, 2018年10月.

(キーワード): 薬物性歯肉増殖症 / カルシウム拮抗薬 / 慢性歯周炎

野中康平, 板東美香, 木戸淳一, 稲垣裕司, 坂本英次郎, 成石浩司, 湯本浩通 :
ショーガオールはヒト歯肉線維芽細胞におけるAGEs誘導性のIL-6およびICAM-1産生を抑制する,
第62回秋季日本歯周病学会学術大会, 2018年10月. 平尾功治, 湯本浩通, 大塚邦紘, 石丸直澄, 松尾敬志 :
歯内由来病変と混同しやすい根尖病変様のエックス線透過像を有する症例,
第18回西日本歯内療法学会会研修会(2018年度), 2018年9月. 藤原奈津美, 村上圭史, 吉田賀弥, 工藤保誠, 弘田克彦, 尾崎和美, 藤猪英樹, 湯本浩通 :
MPCポリマーコーティングによる義歯用レジンのカンジダ付着(撥菌)効果,
第60回日本歯科基礎医学会学術大会 2018年9月5-7日(九州大学病院キャンパス百年講堂, 福岡市), 2018年9月. 湯本浩通 :
口腔バイオフィルム感染症における病原因子,
四国歯学会第53回例会, 2018年7月. 西川泰史, 成石浩司, 木戸淳一, 湯本浩通 :
歯肉線維芽細胞におけるカルプロテクチン誘導性炎症関連因子の産生に及ぼす高グルコースの影響,
第148回日本歯科保存学会秋季学術大会, 2018年6月. 蔵本瞳, 湯本浩通, 平尾功治, 細川由樹, 中西正, 武川大輔, 松尾敬志 :
Caffeic Acid Phenetyl Ester (CAPE)の象牙芽細胞と骨芽細胞におけるVEGF産生誘導機序の解析,
第148回日本歯科保存学会春季学術大会, 2018年6月. 坂本英次郎, 高木亮輔, 稲垣裕司, 木戸淳一, 湯本浩通 :
最終糖化産物とLPSは骨細胞由来スクレロスチンを介して骨芽細胞分化を抑制する,
第148回日本歯科保存学会秋季学術大会, 2018年6月. 武川香織, 二宮雅美, 永田俊彦, 湯本浩通 :
心血管疾患を抱えた重度薬物性歯肉増殖症患者に対して歯周基本治療を行った一症例,
日本歯周病学会会誌, Vol.60, 162, 2018年6月.

(キーワード): 薬物性歯肉増殖症 / 歯周基本治療 / 心血管疾患

稲垣裕司, 野中康平, 高木亮輔, 西川泰史, 木戸理恵, 坂本英次郎, 成石浩司, 木戸淳一, 湯本浩通 :
甘露飲エキス配合製剤はラット実験的歯周炎において歯槽骨吸収を抑制する,
第61回春季日本歯周病学会学術大会, 2018年6月. 野中康平, 板東美香, 木戸淳一, 稲垣裕司, 坂本英次郎, 成石浩司, 湯本浩通 :
最終糖化産物はヒト歯肉線維芽細胞におけるIL-6とICAM-1の発現増加を介して単球との接着を誘導する,
第61回春季日本歯周病学会学術大会, 2018年6月. 木戸理恵, 坂本英次郎, 板東美香, 稲垣裕司, 二宮雅美, 美原(和田) 智恵, 生田貴久, 成石浩司, 永田俊彦, 木戸淳一, 湯本浩通 :
インプラント周囲溝滲出液中のカルプロテクチンとNTxを用いたインプラント疾患の診断,
第61回春季日本歯周病学会学術大会, 2018年6月. 高木亮輔, 坂本英次郎, 稲垣裕司, 廣島佑香, 成石浩司, 木戸淳一, 湯本浩通 :
カルプロテクチンはマウス骨細胞の炎症および骨代謝関連因子の発現を制御する,
第61回春季日本歯周病学会学術大会, 2018年6月. 弘田克彦, 湯本浩通, 大野由香, 中石裕子, 野村加代, 坂本まゆみ, 和食沙紀, 濱田美晴, 内田智子 :
口腔病原細菌に焦点をあてた呼吸器疾患重症化に関与するメカニズム,
第91回日本細菌学会総会 & 第14回日韓国際微生物学シンポジウム, 2018年3月. 湯本浩通 :
口腔バイオフィルム感染症における病原因子と全身に及ぼす影響,
日本義歯ケア学会第10回学術大会, 2018年1月. 藤原奈津美, 湯本浩通, 弘田克彦, 村上圭史, 尾崎和美 :
MPC ポリマーによる口腔細菌の抑制効果,
第59回歯科基礎医学会学術大会プログラム・抄録集平成29年9月16-18日(松本歯科大学，長野県塩尻市), 341, 2017年9月. 平尾功治, 蔵本瞳, 湯本浩通, 細川由樹, 松尾敬志 :
プロポリス生理活性物質(CAPE; Caffeic Acid Phenetyl Ester)を用いた新規覆髄材の開発,
第17回西日本歯内療法学会研修会, 2017年9月. 平尾功治, 湯本浩通, 細川由樹, 蔵本瞳, 松尾敬志 :
ヒト歯髄組織ならびにラット象牙芽細胞(KN-3)におけるMincle発現と象牙芽細胞での機能解析,
第38回日本歯内療法学会学術大会 & 第14回日韓合同歯内療法学会学術大会, 2017年7月. 蔵本瞳, 湯本浩通, 平尾功治, 細川由樹, 中西正, 武川大輔, 松尾敬志 :
Caffeic Acid Phenethyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生に与える影響,
日本歯科保存学会2017年度春季学術大会(第146回), 2017年6月. 湯本浩通 :
口腔レンサ球菌の全身(特に宿主免疫)に及ぼす影響,
第71回日本口腔科学会学術集会, 2017年4月. 湯本浩通 :
高周波・電磁波照射の歯周組織再生療法への応用,
第75回日本矯正歯科学会大会, 2016年11月. 松井沙織, 呉本勝隆, 米田直道, 恵比須繁之, 湯本浩通, 的場一成, 石本卓也, 中野貴由, 野杁由一郎, 林美加子 :
新規開発したラット根管治療モデルを用いた電磁波根尖療法の評価,
日本歯科保存学会2016年度秋季学術大会(第145回), 2016年10月. 藤原奈津美, 村上圭史, 湯本浩通, 弘田克彦, 三宅洋一郎 :
歯周病原菌に対する新規化合物の効果,
第69回日本細菌学会中国・四国支部総会平成28年10月15-16日香川県高松市かがわ国際会議場, 38, 2016年10月. 湯本浩通, 平尾功治, 細川由樹, 蔵本瞳, 松尾敬志 :
40年以上前に外傷を受けた根未完成歯の根尖部に硬組織形成の誘導を試みた症例,
第16回西日本歯内療法学会研修会, 2016年9月. 藤原奈津美, 村上圭史, 湯本浩通, 弘田克彦, 尾崎和美, 三宅洋一郎 :
歯周病原菌に対する新規化合物の効果,
第58回歯科基礎医学会学術大会北海道札幌市 2016年8月24-26日, 2016年8月. 細川由樹, 湯本浩通, 平尾功治, 松尾敬志 :
ラット象牙芽細胞様細胞(KN-3)に対するポリフェノールの炎症抑制効果,
第37回日本歯内療法学会学術大会, 2016年7月. 平尾功治, 湯本浩通, 細川由樹, 松尾敬志 :
Streptococci由来Histone-Like DNA binding protein (HLP)刺激の単球様細胞(THP-1)におけるMincle発現への影響,
日本歯科保存学会 2016年度春季学術大会(第144回), 2016年6月. 湯本浩通, 平尾功治, 細川由樹, 松尾敬志 :
Streptococci由来Histone-Like DNA binding proteinの分泌機構と病原因子としての呼吸器感染症における役割,
日本歯科保存学会 2016年度春季学術大会(第144回), 2016年6月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 天羽崇, 三宅洋一郎 :
口腔常在菌Streptococcus intermediusが呼吸器疾患重症化に関与するメカニズム,
第89回日本細菌学会総会, 2016年3月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 細川由樹, 松尾敬志 :
Interleukin-17がラット象牙芽細胞様細胞(KN-3)のCCL20産生に及ぼす影響,
日本歯科保存学会 2015年度秋季学術大会(第143回), 2015年11月. 細川由樹, 湯本浩通, 平尾功治, 中西正, 武川大輔, 松尾敬志 :
象牙芽細胞が有するMincleを介した視細胞認識機構とその歯髄炎症反応における役割,
日本歯科保存学会 2015年度秋季学術大会(第143回), 2015年11月. 湯本浩通, 平尾功治, 富永敏彦, 坂東直樹, 細川由樹, 松尾敬志 :
外科的歯内療法の依頼を受けた根側病変に対して高周波・電磁波照射を応用した症例,
第15回日本歯内療法学会西日本支部会研修会, 2015年9月. Liu Dali, Zhou Yanbin, Li Yunpeng, Hiromichi Yumoto, Yoichiro Miyake, Liang Jingping and Shu Rong :
Pan-genome and comparative genome of 16 Porphyromonas gingivalis strains,
第58回秋季日本歯周病学会学術大会, Sep. 2015. 富永敏彦, 高比良一輝, 北池光希, 坂東直樹, 湯本浩通, 平尾功治, 松尾敬志 :
EMAT (Electro-Magnetic Apical Treatment)∼根尖性歯周炎への応用に関する検討,
第36回日本歯内療法学会学術大会 & 第13回日韓合同歯内療法学会学術大会, 2015年7月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 天羽崇, 三宅洋一郎 :
Streptococcus intermedius biofilm componentsの糖鎖結合能と炎症反応誘導に関する解析,
第29回日本バイオフィルム学術集会, 2015年7月. 平尾功治, 湯本浩通, 細川由樹, 中西正, 武川大輔, 松尾敬志 :
ラット象牙芽細胞(KN-3)の自然免疫反応におけるシグナル伝達経路の解析,
日本歯科保存学会2015年度春季学術大会(第142回), 2015年6月. 細川由樹, 湯本浩通, 平尾功治, 中西正, 武川大輔, 松尾敬志 :
ラット象牙芽細胞(KN-3)に対するカテキンとカフェイン酸の抗炎症作用,
日本歯科保存学会2015年度春季学術大会(第142回), 2015年6月. 松尾敬志, 中西正, 湯本浩通, 篠原千尋, 平尾功治 :
感染根管治療の考え方,
四国歯学会(第46回例会), 2015年3月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 天羽崇, 三宅洋一郎 :
Streptococcus intermedius Histone-Like Proteinと細胞外ベシクルの特性,
第88回日本細菌学会総会, 2015年3月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 松尾敬志 :
Interleukin-17がヒト歯髄線維芽細胞の炎症メディエーター発現に及ぼす影響,
日本歯科保存学会2014年度秋季学術大会(第141回), 2014年10月. 細川由樹, 湯本浩通, 平尾功治, 高橋加奈子, 松尾敬志 :
イグサ抽出液のオーラルヘルスケア製品への応用に関する研究,
日本歯科保存学会2014年度秋季学術大会(第141回), 2014年10月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 天羽崇, 松尾敬志, 三宅洋一郎 :
Streptococcus intermedius Histone-Like DNA Binding Proteinの結合解析とExtracellular Vesicleとの関連,
第67回日本細菌学会中国・四国支部総会, 2014年10月. 太良尾浩生, 湯本浩通, 平尾功治, 富永敏彦, 坂東直樹, 高比良一輝, 榎本崇宏, 芥川正武, 池原敏孝, 松尾敬志, 木内陽介 :
電磁波照射による骨芽細胞培養培地内の電流密度分布とVEGF産生との関連,
第37回日本生体医工学会中国四国支部大会, 2014年10月. 湯本浩通, 平尾功治, 富永敏彦, 坂東直樹, 高比良一輝, 太良尾浩生, 榎本崇宏, 芥川正武, 池原敏孝, 松尾敬志, 木内陽介 :
電磁波照射による骨芽細胞の活性化と成長因子の発現・産生誘導,
第37回生体医工学会中国四国支部大会, 2014年10月. 平尾功治, 湯本浩通, 中西正, 篠原千尋, 高橋加奈子, 武川大輔, 細川由樹, 松尾敬志 :
ラット象牙芽細胞様細胞(KN3)における細菌関連病原因子刺激に対する自然免疫反応の解析,
日本歯科保存学会2014年度春季学術大会(第140回), 2014年6月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Keiji Murakami and Yoichiro Miyake :
Extracellular DNA influences pyocyanin production from Pseudomonas aeruginosa,
第87回日本細菌学会総会, Mar. 2014. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 三宅洋一郎 :
抗Si-HLP抗体のミトコンドリアATP synthase β subunitに対する交差反応性,
第87回日本細菌学会総会, 2014年3月. 平尾功治, 湯本浩通 :
細菌関連病原因子によりラット象牙芽細胞の自然免疫反応の解析,
第87回日本細菌学会総会, 2014年3月. 木村智子, 尾崎和美, 湯本浩通, 村上圭史, 菅原千恵子, 篠原千尋, 武川恵美, 三宅洋一郎, 松尾敬志, 河野文昭 :
Streptococcus mutans の病原性における scrA 遺伝子の役割,
第6回日本総合歯科学会総会・学術大会, 38, 2013年11月. 湯本浩通, 平尾功治, 松尾敬志 :
イグサ抽出液のう蝕および歯周病予防効果の評価,
第139回日本歯科保存学会秋季学術大会, 2013年10月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake :
Homologous and heterologous extracellular DNAs affect Pseudomonas aeruginosa to produce more pyocyanin without inducing bacterial growth,
第66回日本細菌学会中国・四国支部総会, Oct. 2013. 堀内信也, 湯本浩通, 木村智子, 天知良太, 金南希, 田中栄二 :
パルス式超音波歯ブラシがStreptococcus mutansのBiofilmに及ぼす影響,
第72回日本矯正歯科学会大会プログラム・抄録集, 2013年10月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa,
第27回Bacterial Agherence & Biofilm学術集会, Jul. 2013. 湯本浩通, 富永敏彦, 坂東直樹, 平尾功治, 松尾敬志 :
高周波・電磁波照射の歯内療法への応用ー基礎実験∼臨床症例ー,
第13回日本歯内療法学会西日本支部会研修会, 2013年7月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 高橋加奈子, 松尾敬志, 三宅洋一郎 :
Streptococcus intermediusヒストン様タンパク質のPAMPsとしての解析,
第22回Lancefieldレンサ球菌研究会&第45回レンサ球菌感染症研究会, 2013年6月. 富永敏彦, 湯本浩通, 坂東直樹, 平尾功治, 平尾早希, 加古晋也, 前田やよい, 山口由美子, 高比良一輝, 松尾敬志 :
Electro-Magnetic Apical Treatment ー嚢胞性疾患に対する対応ー,
第138回日本歯科保存学会春季学術大会, 2013年6月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake :
The role of extracellular DNA in Pseudomonas aeruginosa and Streptococcus intermedius infection,
第86回日本細菌学会総会, Mar. 2013. Katsuhiko Hirota, Hiromichi Yumoto, Nur Asikin, Kouji Hirao, Keiji Murakami and Yoichiro Miyake :
Pathogenic roles of Streptococcus intermedius histone-like protein in microbial inflammation,
第86回日本細菌学会総会, Mar. 2013. Nur Asikin, 弘田克彦, 湯本浩通, 平尾功治, 高橋加奈子, 村上圭史, 三宅洋一郎 :
Biological roles of extracellular DNA of Streptococcus intermeius,
第65回日本細菌学会中国・四国支部総会, 2012年10月. 湯本浩通, 富永敏彦, 平尾功治, 松尾敬志 :
電磁波照射による骨芽細胞の活性化とGrowth Factorの発現誘導,
第10回日本再生歯科医学会学術大会, 2012年9月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Role of Extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermeius,
第26回Bacterial Adherence & Biofilm学術集会, Jul. 2012. 中西正, 武川大輔, 平尾功治, 湯本浩通, 高橋加奈子, 松尾敬志 :
prostaglandin F2aは歯髄細胞における炎症性メディエーター発現を調節する,
第136回日本歯科保存学会春季学術大会, 2012年6月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Roles of extracellular DNA and DNA-binding protein in cell aggregation, biofilm formation and antibiotic tolerance of Streptococcus intermedius,
第21回Lancefieldレンサ球菌研究会&第44回レンサ球菌感染症研究会合同開催, Jun. 2012. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami and Yoichiro Miyake :
The Effect of Extracellular DNA on The Development of Streptococcus intermedius Biofilm,
第64回日本細菌学会中国・四国支部総会, Oct. 2011. 湯本浩通, 富永敏彦, 平尾功治, 高橋加奈子, 松尾敬志 :
高周波・電磁波照射による骨芽細胞のGrowth Factorの発現・産生誘導,
第135回日本歯科保存学会秋季学術大会, 2011年10月. 尾崎和美, 藤原奈津美, 石川真琴, 細川育子, 細川義隆, 湯本浩通 :
う蝕関連細菌の家族内伝播と生活習慣による影響に関する研究 ―兄弟姉妹間の差異について―,
134回日本歯科保存学会春季学術大会, 2011年6月. 武川大輔, 中西正, 平尾功治, 湯本浩通, 高橋加奈子, 松尾敬志 :
ヒト培養歯髄細胞の自然免疫応答に対するインターフェロンγの影響,
第134回日本歯科保存学会春季学術大会, 2011年6月. 湯本浩通, 富永敏彦, 柴田眞吾, 武川大輔, 高橋加奈子, 木村智子, 平尾功治, 松尾敬志 :
高周波・電磁波照射を歯内療法に応用した症例,
第134回日本歯科保存学会春季学術大会, 2011年6月. 近藤洋史, 小野恒子, 弘田克彦, 村上圭史, 三宅洋一郎, 湯本浩通, 高橋加奈子, 平尾功治, 松尾敬志 :
THP-1細胞のケモカイン産生に及ぼす口腔レンサ球菌ヒストン様DNA結合タンパク質の影響,
第34回徳島県医学検査学会, 2010年12月. 湯本浩通, 高橋加奈子, 平尾功治, 松尾敬志 :
Streptococcus intermedius由来histon様DNA結合タンパク質がTHP-1細胞のサイトカインバランスに及ぼす影響,
第133回日本歯科保存学会秋季学術大会, 2010年10月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 高橋加奈子, 松尾敬志 :
ヒト歯髄細胞におけるサイトカイン発現に対するProstaglandin F2alpha の影響,
日本歯科保存学会2010年度秋季学術大会(第133回), 2010年10月. 平尾功治, 湯本浩通, 弘田克彦, 高橋加奈子, 木村智子, 三宅洋一郎 :
Streptococcus intermedius由来histon様DNA結合タンパク質がヒト単球系細胞のサイトカイン・ケモカイン産生に及ぼす影響,
第63回日本細菌学会中国・四国支部総会, 2010年10月. 住友孝史, 富永敏彦, 坂東直樹, 平尾早希, 湯本浩通, 松尾敬志 :
Electro-Magnetic Apical Treatmentを行った1例,
第31回日本歯内療法学会学術大会, 2010年7月. 弘田克彦, 湯本浩通, 星野由美, 村上圭史, 宮本幸治, 松尾敬志, 三宅洋一郎 :
水溶性MPCポリマーの口腔細菌付着抑制効果,
Bacterial Adherence &Biofilm学術集会, 2010年7月. 坂東直樹, 富永敏彦, 住友孝史, 湯本浩通, 松尾敬志 :
難治性根尖性歯周炎に対する電磁波の応用-EMAT (Electro-Magnetic Apical Treatment)-,
第10回日本歯内療法学会西日本支部研修会, 2010年7月. 藤田剛, 應原一久, 湯本浩通, 林田浩一, 柴秀樹, 岩田倫幸, 内田雄士, 宮川剛史, 河口浩之, 松尾敬志, 栗原英見 :
イソグラジンマレイン酸による歯肉上皮細胞のバリアー機能制御について,
第132回日本歯科保存学会春季学術大会, 2010年6月. 藤田剛, 應原一久, 湯本浩通, 林田浩一, 永原隆吉, 宮川剛史, 岩田倫幸, 柴秀樹, 河口浩之, 松尾敬志, 栗原英見 :
Aggregatibacter actinomycetemcomitansが歯肉上皮細胞のclaudin-1発現および機能に及ぼす影響,
第53回春季日本歯周病学会学術大会, 2010年5月. 湯本浩通, 平尾功治, 木村智子, 高橋加奈子 :
口腔病原菌に対する高周波・電磁波照射の殺菌効果,
第83回日本細菌学会総会, 2010年3月. 高橋加奈子, 湯本浩通, 平尾功治, 木村智子 :
培養歯髄細胞におけるAlarminの産生・放出機構の解析,
第83回日本細菌学会総会, 2010年3月. 木村智子, 尾崎和美, 湯本浩通, 村上圭史, 鹿山鎭男, 藤坂菊美, 高橋加奈子, 三宅洋一郎 :
Streptococcus mutansの病原性におけるscrAおよびhtrA遺伝子の役割,
第83回日本細菌学会総会, 2010年3月. 弘田克彦, 鹿山鎭男, 村上圭史, 湯本浩通, 三宅洋一郎 :
Intermedilysinによる小胞体異常の分子機構の解明,
第83回日本細菌学会総会, 2010年3月. 弘田克彦, 鹿山鎭男, 湯本浩通, 三宅洋一郎 :
Intermedilysinに誘導されるHuCCT1細胞gp210の分布変動,
第62回日本細菌学会中国・四国支部総会, 2009年10月. 弘田克彦, 鹿山鎭男, 湯本浩通, 田端厚之, 長宗秀明, 小野恒子, 三宅洋一郎 :
Intermedilysinにより誘導される小胞体異常とgp210の細胞内分布の変動,
第82回日本日本細菌学会総会, 2009年3月. 劉大力, 周彦王分, 湯本浩通, 鹿山鎭男, 李超倫, 梁景平, 三宅洋一郎, 束蓉 :
Purification of recombinant Porphyromonas gingivalis DnaK and creation of its mutant,
第82回日本日本細菌学会総会, 2009年3月. 弘田克彦, Heni Susilowati, 岡村裕彦, 吉田賀弥, 田端厚之, 鹿山鎭男, 湯本浩通, 羽地達次, 長宗秀明, 小野恒子, 三宅洋一郎 :
Intermedilysinにより誘導されるカルシウム振動と胆管上皮細胞死,
第17回Lancefielfレンサ球菌研究会 2008, 2008年7月. 平尾功治, 湯本浩通, 向井佳代, 松尾敬志 :
カテキンのヒト培養歯髄細胞に対する抗炎症効果,
第29回日本歯内療法学会, 2008年5月. 富永敏彦, 湯本浩通, 松尾敬志 :
Electro-Magnetic Apical Treatment,
第29回日本歯内療法学会, 2008年5月. 木村智子, 尾崎和美, 鹿山鎭男, 藤坂菊美, 湯本浩通, 三宅洋一郎 :
Streptococcus mutansの病原性におけるスクロース輸送系遺伝子の役割について,
第81回日本細菌学会総会, 2008年3月. 平尾功治, 高橋加奈子, 湯本浩通 :
ヒト培養歯髄細胞における自然免疫反応の解析とカテキンによる抑制効果,
第81回日本細菌学会総会, 2008年3月. Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
The essentiality and involvements of Streptococcus intermedius histone-like protein in bacterial viability and normal growth,
The 2nd international symposium on "The future direction of oral sciences in the 21st century", Dec. 2007. 平尾功治, 湯本浩通, 向井佳代, 高橋加奈子, 中西正, 松尾敬志 :
カテキンは細菌やその構成成分に対する培養歯髄細胞の反応を調節する,
第127回日本歯科保存学会秋季学術大会, 2007年11月. 湯本浩通, 平尾功治, 高橋加奈子, 向井佳代, 中西正, 松尾敬志 :
培養歯髄細胞におけるPattern Recognition Receptor (PRR)を介した接着分子の発現,
第127回日本歯科保存学会秋季学術大会, 2007年11月. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Proinflammatory Roles of NOD2 in Human Gingival Fibroblasts,
第55回国際歯科研究学会日本部会(JADR)総会・学術大会, Nov. 2007. Dali LIU, 村上圭史, 湯本浩通, 弘田克彦, 三宅洋一郎 :
Investigation of the toxicity of Streptococcus intermedius histon-like protein in Escherichia coli,
第60回日本細菌学会中国・四国支部総会, 2007年10月. Dali LIU, 湯本浩通, 松尾敬志, 三宅洋一郎 :
Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via activation of ERK1/2 and JNK pathways.,
日本歯周病学会50周年記念大会, 2007年9月. 米田美穂, 中江英明, 湯本浩通, 篠原千尋, 松尾敬志 :
炎症性サイトカインがヒト歯肉上皮細胞間接着機構に及ぼす影響,
第50回日本歯周病学会春季学術大会, 2007年5月. LIU Dali, Keiji Murakami, Hiromichi Yumoto, Katsuhiko Hirota and Yoichiro Miyake :
Investigation of the essentiality of histone-like protein in Streptococcus intermedius,
第80回日本細菌学会総会, Mar. 2007. 劉大力, 村上圭史, 湯本浩通, 弘田克彦, 三宅洋一郎 :
Investigation of the Essentiality of Histone-Like Protein in Streptococcus intermedius,
第80回日本細菌学会総会, 2007年3月. 平尾功治, 湯本浩通, 高橋加奈子, 向井佳代, 松尾敬志 :
う蝕関連細菌が歯髄細胞における自然免疫応答に及ぼす影響,
第125回日本歯科保存学会秋季学術大会, 2006年11月. LIU Dali, Hiromichi Yumoto, Kanako Takahashi, Keiji Murakami, Katsuhiko Hirota and Yoichiro Miyake :
Proinflammatory cytokines are induced in human monocytes in response to exposure to the histone like protein of Streptococcus intermedius,
第59回日本細菌学会中国・四国支部総会, Oct. 2006. 平尾功治, 高橋加奈子, 湯本浩通 :
培養歯髄細胞におけるPRR分子の解析,
第59回日本細菌学会中国・四国支部総会, 2006年10月. 米田美穂, 中江英明, 湯本浩通, 篠原千尋, 松尾敬志 :
歯周病変局所における上皮細胞間接着機構の動態,
第124回日本歯科保存学会春季学会, 2006年5月. 平尾功治, 湯本浩通, 高橋加奈子, 足立智彦, 向井佳代, 松尾敬志 :
培養歯髄細胞におけるPattern Recognition Receptor (PRR)の発現と機能解析,
第124回日本歯科保存学会春季学術大会, 2006年5月. 足立智彦, 平尾功治, 湯本浩通, 高橋加奈子, 向井佳代, 中西正, 中江英明, 松尾敬志 :
歯髄組織および細菌刺激THP-1細胞におけるCXCR3 ligands(特にIP-10)の発現解析,
第123回秋季日本歯科保存会, 2005年11月. 高橋加奈子, 中西正, 山口大輔, 細川義隆, 湯本浩通, 尾崎和美, 中江英明, 松尾敬志 :
歯髄炎の病態形成におけるLARCおよびCCR6の役割,
第32回日本免疫学会, 2002年12月. 湯本浩通, 弘田克彦, 長宗秀明, 大倉一人, 村上圭史, 山田真義, 中江英明, 三宅洋一郎 :
Streptococcus intermedius hitone-like proteinをコードする遺伝子のクローニングと発現,
第75回日本細菌学会総会, 2002年4月. 山口大輔, 細川義隆, 篠原千尋, 湯本浩通, 中西正, 中江英明, 松尾敬志 :
歯周炎病変局所におけるFractalkineおよびCX3CR1の発現,
第45回春季日本歯周病学会, 2002年4月. 山下美由紀, 渡邉久恵, 三木千津, 石川清美, 久保万友美, 蓮井義則, 湯本浩通, 松尾敬志 :
歯科衛生士の卒後研修の重要性とその意義,
第45回春季日本歯周病学会, 2002年4月. 山下美由紀, 渡邉久恵, 三木千津, 石川清美, 久保万友美, 蓮井義則, 湯本浩通, 松尾敬志 :
歯科衛生士の卒後研修の重要性とその意義,
香川県歯科医学大会, 2002年2月. 久保万友美, 山下美由紀, 渡邉久恵, 三木千津, 石川清美, 蓮井義則, 湯本浩通, 松尾敬志 :
在宅患者の口腔ケアにおける歯科衛生士の役割,
香川県歯科医学大会, 2002年2月. 山口大輔, 細川義隆, 湯本浩通, 中西正, 中江英明, 松尾敬志 :
歯周炎病変局所におけるIL-18とその機能に関連する分子の発現について,
第31回日本免疫学会学術集会, 2001年12月. 高橋加奈子, 中西正, 細川義隆, 篠原千尋, 湯本浩通, 尾崎和美, 松尾敬志 :
炎症歯髄におけるLARCおよびCCR6の発現,
第115回秋季日本歯科保存学会, 2001年11月. 細川義隆, 中西正, 山口大輔, 清水洋利, 湯本浩通, 尾崎和美, 松尾敬志 :
歯周炎病変局所におけるLARCおよびCCR6の発現,
第44回秋季日本歯周病学会学術大会, 2001年10月. 湯本浩通, 弘田克彦, 山田真義, 中江英明, 村上圭史, 三宅洋一郎, 松尾敬志 :
Streptococcus intermedius histone-like proteinをコードする遺伝子のクローニングと発現,
第44回秋季日本歯周病学会学術大会, 2001年10月. 久保万友美, 渡邉久恵, 三木千津, 石川清美, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
在宅患者の口腔ケアにおける歯科衛生士の役割,
第44回秋季日本歯周病学会学術大会, 2001年10月. 山田眞義, 湯本浩通, 篠原千尋, 中江英明, 松尾敬志 :
歯周病関連細菌Eikenella corrodensが血管内皮細胞に及ぼす影響,
四国歯学会, 2001年6月. 山田眞義, 湯本浩通, 篠原千尋, 中江英明, 恵比須繁之 :
Eikenella corrodensによる血管内皮細胞増殖促進と接着分子・増殖因子発現誘導,
第74回日本細菌学会総会, 2001年4月. 湯本浩通, 篠原千尋, 山田眞義, 中江英明, 恵比須繁之 :
Eikenella corrodens培養上清による血管内皮細胞活性化機序の解析,
第74回日本細菌学会総会, 2001年4月. 三木千津, 渡邊久恵, 石川清美, 石川利栄子, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
歯科衛生士の海外卒後研修に参加して -歯周組織・口腔粘膜疾患と国際協力についてー,
第44回春季日本歯周病学会学術大会, 2001年4月. 山口大輔, 細川義隆, 清水洋利, 篠原千尋, 湯本浩通, 中西正, 松尾敬志 :
歯周炎の病変局所におけるIL-18およびIL-18レセプターの発現,
第44回春季日本歯周病学会学術大会, 2001年4月. 尾崎和美, 湯本浩通, 篠原千尋, 木持健, 松尾敬志 :
象牙質齲蝕に侵入した細菌種の同定, --- 電顕用樹脂包埋試料から抽出した16S rDNAを用いて ---,
第113回日本歯科保存学会秋季学会, 2000年11月. 湯本浩通, 篠原千尋, 山田真義, 中江英明, 恵比須繁之, 松尾敬志 :
Eikenella corrodens培養上清による血管内皮細胞の活性化機序の解析,
第43回秋季日本歯周病学会学術大会, 2000年10月. 石川清美, 渡邊久恵, 三木千津, 久保万友美, 石丸毅, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
歯周病患者に対する禁煙指導について -ニコチネルの応用―,
第43回秋季日本歯周病学会学術大会, 2000年10月. 弘田克彦, 湯本浩通, 村田広美, 大倉一人, 長宗秀明, 根本謙, 小野恒子, 三宅洋一郎 :
レンサ球菌のヒストン様蛋白質の構造解析,
第53回日本細菌学会中国・四国支部総会, 2000年10月. 山田眞義, 中江英明, 湯本浩通, 篠原千尋, 藤中恵子, 松尾敬志 :
上皮細胞のICAM-1発現誘導におけるEikenella corrodens付着因子の関与について,
第43回春季日本歯周病学会学術大会, 2000年5月. 久保万友美, 渡邊久恵, 三木千津, 石川清美, 石丸毅, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
瘢痕性類天疱瘡を有する患者の口腔管理,
第43回春季日本歯周病学会学術大会, 2000年5月. 山田真義, 湯本浩通, 村上圭史, 中江英明 :
上皮細胞のICAM-1発現誘導におけるEikenella corrodens付着因子の関与について,
第73回日本細菌学会総会, 2000年5月. 湯本浩通, 山田真義, 中江英明, 恵比須繁之 :
Eikenella corrodensによる上皮細胞のMitogen-Activated Protein Kinase (MAPK)活性化,
第73回日本細菌学会総会, 2000年5月. 三木千津, 石川清美, 渡邊久恵, 石丸毅, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
臼歯部の動揺を伴う歯周病患者への対応 -特にメインテナンスにおける衛生士の役割―,
第42回秋季日本歯周病学会学術大会, 1999年10月. 吉山昌宏, 篠原千尋, 湯本浩通, 松尾敬志 :
X線微小分析法による象牙質接着界面の解析,
第105回日本歯科保存学会秋季学会, 1996年11月.

研究会・報告書

湯本浩通, 二宮雅美 :
薬物性歯肉増殖症,
健康と生活, No.546, 2023年9月.

(キーワード): 歯周病 (periodontitis) / 副作用 (side effects)

湯本浩通 :
PCRを用いた歯周病原細菌迅速検出システムの有用性(WEB),
Orcoa.seminar (Doctorbook Academy), 2023年8月. 湯本浩通 :
口腔バイオフィルム感染症-う蝕や歯周病-とその予防法,
アークレイSillHa Webセミナー2023 徳島(WEB), 2023年4月. 湯本浩通 :
技術革新による歯内療法の新潮流,
2023年日本歯内療法学会(JEA)研修会(大阪), 2023年3月. 湯本浩通 :
歯周病の新分類と検査(PISA)について,
香川県歯科衛生士復職支援研修会, 2022年12月. Hiromichi Yumoto :
The effects of Climate Change on Oral Health and Dental Practice,
Summer Course 2022 on Interprofessional Healthcare Environmental Sustainability for Healthier and Happier Life (Indonesia/WEB), Oct. 2022. 湯本浩通 :
糖尿病と歯周病の関連性,
令和4年度認定歯科衛生士セミナー「糖尿病予防指導」コースプログラム, 2022年10月. 湯本浩通 :
歯周病と象牙質知覚過敏症との関連,
GSKヘルスパートナーウェビナー講演会, 2022年9月. Hiromichi Yumoto :
Current Clinical Techniques in Endodontics,
The Expert Lecture of the Conservative Dentistry Specialist Study Program (University of Gadjah Mada, Indonesia/WEB), Sep. 2022. 湯本浩通 :
糖尿病と歯周病,
令和3年度徳島県糖尿病療養指導士研修会, 2022年3月. 湯本浩通 :
高齢者の口腔機能管理-歯周病とオーラルフレイル予防の観点から-,
第8回多職種のための在宅医療研修会, 2021年11月. 湯本浩通 :
糖尿病と歯周病,
令和3年度第3回糖尿病療養指導メディカルゾーンセミナー, 2021年11月. 湯本浩通 :
糖尿病と歯周病の関連性,
認定歯科衛生士セミナー「糖尿病予防指導」コースプログラム, 2021年10月. 湯本浩通 :
糖尿病と歯周病,
令和2年度徳島県糖尿病療養指導士研修会, 2021年3月. 湯本浩通 :
歯周病のトピックス―検査・評価法・新分類と抗菌薬適正使用について―,
徳島県歯科医師会学術講演会, 2021年1月. 植村勇太, 廣島佑香, 村上圭史, 多田彩乃, 桑原知巳, 藤猪英樹, 湯本浩通 :
Porphyromonas gingivalis由来メンブレンベシクルがヒト歯肉上皮細胞に及ぼす影響,
徳島大学研究クラスター Joint Meeting on Microbiology 2020, 2020年11月. 秋月皆人, 村上圭史, 廣島佑香, 藤猪英樹, 湯本浩通 :
MPCポリマーコーティングにおけるTi表面への歯周病原菌付着抑制効果の検討,
徳島大学研究クラスター Joint Meeting on Microbiology 2020, 2020年11月. 山口舜祐, 竹谷英雄, 芥川正武, 湯本浩通, 平尾功治, 榎本崇宏, 太良尾浩生, 富永敏彦, 池原敏孝, 安野恵実子, 木内陽介 :
高周波電圧印加による殺菌と骨形成への影響,
LED総合フォーラム2020 in 徳島, 113-116, 2020年2月. 湯本浩通, 太良尾浩生, 平尾功治, 富永敏彦, 坂東直樹, 高比良一輝, 榎本崇宏, 芥川正武, 池原敏孝, 松尾敬志, 木内陽介 :
電磁波照射による骨芽細胞培養培地内の電流密度分布とVEGF産生との関連,
LED総合フォーラム2020 in 徳島, 2020年2月. 湯本浩通 :
糖尿病と歯周病,
令和元年度徳島県糖尿病療養指導士研修会, 2019年12月. 湯本浩通 :
糖尿病と歯周病の関連ー細菌感染と炎症のコントロールの重要性ー,
医科歯科連携講演会(徳島県歯科医師会), 2019年11月. 湯本浩通 :
糖尿病と歯周病の関連性,
認定歯科衛生士セミナー・認定分野A・糖尿病予防指導プログラム, 2019年9月. 湯本浩通 :
天然歯保存における歯内療法の重要性と再生療法の可能性,
徳島大学歯学部同窓会関東支部令和元年度学術講演会, 2019年7月. Hiromichi Yumoto :
The clinical updates in Japan, especially about the new system using the recombinant FGF (Regroth),
Gajdjah Mada University, Mar. 2019. Hiromichi Yumoto :
The clinical updates in Japan, especially about the new system using the recombinant FGF (Regroth),
Universitas Muhammadiyah Yogyakarta, Mar. 2019. Hiromichi Yumoto :
The Association between periodontal Diseases and Systemic Diseases, especially Diabetes,
Universitas Muhammadiyah Yogyakarta, Mar. 2019. 湯本浩通 :
歯周病!全身の病気との関係と口腔ケア,
平成30年度冬版健保連生活習慣改善セミナー, 2019年2月. 湯本浩通 :
糖尿病と歯周病,
平成30年度徳島県糖尿病療養指導士研修会, 2019年1月. 二宮雅美, 坂本英次郎, 成石浩司, 湯本浩通 :
周期性好中球減少症患者に発症した重度歯周炎の家族症例,
第1回侵襲性歯周炎センタークリニカルカンファレンス, 2018年12月.

(キーワード): 周期性好中球減少症 / 歯周炎 (periodontal disease) / 歯周治療

藤原奈津美, 鶴熊美樹, 吉田賀弥, 村上圭史, 尾崎和美, 藤猪英樹, 湯本浩通 :
MPCポリマーコーティングによる義歯用レジンのカンジダ付着抑制効果,
2018 Bioscience retreat in Awaji, 2018年11月. 湯本浩通 :
現代の歯内療法の概念と今後の再生治療の可能性,
徳島大学歯学部同窓会香川県支部平成30年度学術講演会, 2018年11月. 湯本浩通 :
歯周病の重症化予防と継続管理,
かかりつけ歯科医機能強化型歯科診療所研修会(徳島県歯科医師会), 2018年10月. 湯本浩通 :
成功へ導くための現代の歯内療法の概念と今後の再生治療の可能性,
大阪府歯科医師会学術講演, 2018年7月. 湯本浩通 :
現代の歯内療法と今後の再生治療の可能性,
徳島大学歯学部同窓会平成29年度卒後研修セミナー, 2018年3月. Hiromichi Yumoto :
Current non-surgical method to treat periapical periodontitis; Etiology and pathology of refractory periapical periodontitis,
Gadjah Mada University, Aug. 2017. Hiromichi Yumoto :
Inhibition of bacterial activity, inactivation of oral pathogen and activating effect of electromagnetic wave irradiation,
Gadjah Mada University, Aug. 2017. 湯本浩通 :

平成23年度再生工学カテゴリー研究報告会(連携機能を活用した口腔からQOL向上を目指す研究), 2012年1月. Hiromichi Yumoto :
The Relationship between Periodontitis and Cardiovascular Diseases,
上海交通大学, 2008. 弘田克彦, 湯本浩通, 村田広美, Viducic Darijia, 谷口和子, 村上圭史, 根本謙, 小野恒子, 三宅洋一郎 :
Staphyrococcus aureusにおけるhistone-like proteinの存在と構造解析,
第46回ブドウ球菌研究会, 2001年9月. 蓮井義則, 渡邊久恵, 湯本浩通, 篠崎文彦 :
小学校全児童を対象にした経年的な歯科検診・検査の解析,
第12回中四国小児口腔外科学臨床研究会, 2001年6月. 弘田克彦, 村田広美, 湯本浩通, 大倉一人, 長宗秀明, 三宅洋一郎 :
レンサ球菌のヒストン様蛋白質の構造解析,
Bacterial Adherence & Biofilm, Vol.14, 6-8, 2000年1月.

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 再生環境下での休眠状態・若返り間葉系幹細胞の歯周組織再生療法と全身疾患への応用 (研究課題/領域番号: 24K12913 )
糖尿病患者の口腔保健行動セルフケア支援に向けた看護プログラムの再改編 (研究課題/領域番号: 23K09954 )
歯周病が惹起する全身炎症性疾患の機序解明と酸化ストレス制御による予防法確立 (研究課題/領域番号: 23K09333 )
ポリフェノール内包キトサンナノカプセルを用いた口腔バイオフィルム抑制技術の開発 (研究課題/領域番号: 22K09967 )
歯周医学における歯周病原細菌由来ベシクルの役割解明と歯周病重症化予防剤の開発 (研究課題/領域番号: 21K09896 )
糖尿病関連歯周炎における歯周組織細胞由来エクソソームの総合的研究 (研究課題/領域番号: 21K09895 )
糖尿病患者の口腔保健行動の看護支援プログラムの再編と効果検証-教育教材の開発 (研究課題/領域番号: 20K10755 )
口腔バイオフィルムの分散能と抗菌能を有する光触媒・ポリフェノール合剤の新規開発 (研究課題/領域番号: 19K10132 )
慢性腎臓病患者の歯周病原細菌感染度と推算糸球体濾過量(eGFR)との関係 (研究課題/領域番号: 19K10111 )
細菌感染による死細胞を認識する自然免疫受容体Mincleの歯髄における役割の解明 (研究課題/領域番号: 18K09577 )
訪問・在宅に向けた新しい概念の保存治療法の開発―高周波/電磁波などを応用して― (研究課題/領域番号: 17K11708 )
ポリフェノール・銀ナノ粒子複合体を応用した口腔バイオフィルム形成制御技術の開発 (研究課題/領域番号: 16K11554 )
エピジェネティクス・転写後発現調節機構の解析による新規歯髄温存・石灰化療法の開発 (研究課題/領域番号: 15K11117 )
自然免疫を介した象牙芽細胞石灰化メカニズムの解明 (研究課題/領域番号: 15K11116 )
歯髄炎における炎症・抗炎症バランス制御機構の解析とTh17細胞の役割 (研究課題/領域番号: 15K11115 )
小動物のマイクロエンドモデルの確立と新規根管治療法や根管治療薬の開発に関する研究 (研究課題/領域番号: 15H05021 )
高周波/電磁波治療の深在性齲蝕治療への応用―訪問・在宅治療を目指して― (研究課題/領域番号: 26462885 )
オートインデューサーの分解を機作とした口腔バイオフィルム形成制御に関する研究 (研究課題/領域番号: 25462962 )
菌体外ＤＮＡ・蛋白複合体を標的とした口腔バイオフィルム感染症の予防と治療法の開発 (研究課題/領域番号: 24592872 )
歯髄炎の病態形成における炎症－抗炎症バランス制御の解析 (研究課題/領域番号: 24592871 )
歯髄炎の病態形成における象牙芽細胞の細菌侵襲に対する免疫機構の解明 (研究課題/領域番号: 24592870 )
歯周病変に発現するクローディンとケモカインによる歯周病原因子の上皮透過性制御機構 (研究課題/領域番号: 23593064 )
ミニマムインターベンションの概念と齲蝕病態に基づく新規の包括的齲蝕治療法の開発 (研究課題/領域番号: 23390435 )
アドレノメデュリンの抗菌作用による口腔バイオフィルムの形成抑制効果に関する研究 (研究課題/領域番号: 22592122 )
難治性根尖性歯周炎と根尖孔外バイオフィルムに対する電磁・高周波の歯内療法への応用 (研究課題/領域番号: 21592423 )
歯髄保存療法への緑茶カテキン応用に関する研究 (研究課題/領域番号: 20592228 )
歯周病変特異的に発現するクローディンによる歯周病原因子の上皮透過性制御機構の解明 (研究課題/領域番号: 18592264 )
歯周病によるアテローム性動脈硬化症の惹起・促進メカニズムの解明と原因因子の同定 (研究課題/領域番号: 18592263 )
遺伝子工学的手法を応用した難治性根尖性歯周炎症例の細菌侵襲の実態の解析 (研究課題/領域番号: 18592092 )
歯周病におけるバイオフィルム形成と薬剤耐性遺伝子伝播を制御する遺伝子と蛋白の解析 (研究課題/領域番号: 14771222 )
微小組織切片中の核酸を利用した露髄部の細菌種の同定および炎症歯髄の病態解析 (研究課題/領域番号: 13672000 )
ヒト根尖性歯周炎における根尖孔外への細菌侵襲の実態と宿主の応答 (研究課題/領域番号: 12671853 )
歯周ポケット底部を再現した3次元立体培養系の応用による歯周炎の病態解析 (研究課題/領域番号: 10771218 )
in situ(RT-)PCR法を応用した歯周ポケット底部接合上皮周辺の病態解析 (研究課題/領域番号: 10671968 )
歯周病原性細菌の付着因子複合体を構成する蛋白質の分子生物学的解析 (研究課題/領域番号: 08672194 )
研究者番号（60284303）による検索

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研究者総覧で最新データを確認する

2024年4月26日更新

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)
所属学会・所属協会: 日本歯周病学会
日本歯科保存学会
国際歯科研究学会
日本細菌学会
日本歯内療法学会 [2014年6月〜2022年12月], 代議員 [2018年2月〜2024年7月])
四国歯学会
American Association of Endodontists
歯科基礎医学会
委員歴・役員歴: 日本歯周病学会 (常任理事・理事・評議員 [2018年6月〜2023年3月])
日本歯科保存学会 (評議員 [2013年4月〜2019年3月], 理事 [2018年6月〜2023年3月])
日本歯内療法学会 (理事(西日本歯内療法学会) [2014年6月〜2022年12月], 代議員(日本歯内療法学会) [2018年2月〜2024年7月])
受賞: 1996年1月, 三木康楽賞
1998年12月, 若野洋一歯科臨床医学奨励賞
2000年4月, 優秀ポスター賞 (第74回日本細菌学会総会)
2019年2月, 康楽賞 (財団法人三木康楽会)
2019年6月, 松風優秀ポスター賞 (日本歯科保存学会)
活動: 研究者総覧に該当データはありませんでした。

リサーチマップで最新データを確認するＪグローバルで最新データを確認する

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Ｊグローバル

Jグローバル最終確認日: 2024/4/20 01:32
氏名（漢字）: 湯本浩通
氏名（フリガナ）: ユモトヒロミチ
氏名（英字）: Yumoto Hiromichi
所属機関: 徳島大学教授

リサーチマップ

researchmap最終確認日: 2024/4/21 02:39
氏名（漢字）: 湯本浩通
氏名（フリガナ）: ユモトヒロミチ
氏名（英字）: Yumoto Hiromichi
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登録日時: 2010/11/2 00:00
更新日時: 2024/2/16 15:00
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所属: 徳島大学
部署: 大学院・医歯薬学研究部・歯周歯内治療学分野
職名: 教授
学位: 博士(歯学)
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研究者番号: 60284303

所属（現在）: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 教授

所属（過去の研究課題 情報に基づく）*注記: 2018/4/1 – 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 教授
2017/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学系), 教授
2013/4/1 – 2016/4/1 : 徳島大学, 病院, 講師
2012/4/1 – 2015/4/1 : 徳島大学, 大学病院, 講師
2007/4/1 – 2011/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教
2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助手
2002/4/1 : 徳島大, 歯学部, 助手
1998/4/1 – 2002/4/1 : 徳島大学, 歯学部, 助手
1996/4/1 : 徳島大学, 歯学部・附属病院, 助手

審査区分/研究分野

研究代表者

医学 / 歯学 / 歯周治療系歯学
生物系 / 医歯薬学 / 歯学 / 保存治療系歯学
生物系 / 医歯薬学 / 歯学 / 歯周治療系歯学
小区分57030:保存治療系歯学関連

研究代表者以外

医学 / 歯学 / 保存治療系歯学
生物系 / 医歯薬学 / 歯学 / 保存治療系歯学
生物系 / 医歯薬学 / 歯学 / 歯周治療系歯学
医学 / 歯学 / 歯周治療系歯学
小区分57030:保存治療系歯学関連
小区分58060:臨床看護学関連
小区分57060:外科系歯学関連

キーワード

研究代表者

歯周病 / 歯周病関連細菌 / 上皮細胞 / 血管内皮細胞 / 細胞内シグナル伝達 / サイトカイン / 接着分子 / 細胞増殖因子 / 歯内療法学 / 根尖性歯周炎 / 電磁・高周波 / 細菌 / 骨芽細胞 / 遺伝子発現 / 成長因子 / シグナル伝達 / 歯内療法 / マイクロアレイ / オステオポンティン / バイオフィルム / 歯学 / 感染症 / 細胞・組織 / 遺伝子 / 糖鎖 / 動脈硬化症 / 細菌感染 / 炎症 / マクロファージ / 免疫応 / 炎症性サイトカイン / スカベンジャーレセプター / Periodontal Disease / Atherosclerosis / Periodontopathogen / Bacterial Infection / Inflammation / Macrophage / Gene_Expression / Immune Reaction / 象牙芽細胞 / 自然免疫 / ポリフェノール / ケモカイン / VEGF / Mincle / 歯髄 / シグナル伝達経路 / 受容体 / Outer Membrane Vesicle / 歯肉上皮細胞 / NF-kB / 歯槽骨吸収 / MAPK / 病原細菌 / 歯周医学 / ベシクル / 重症化予防

研究代表者以外

歯周病 / Eikenella corrodens / 付着因子 / 遺伝子クローニング / 遺伝子発現 / モノクローナル抗体 / 外膜蛋白質 / カテキン / 歯髄炎 / 歯髄細胞 / 抗炎症作用 / サイトカイン / ケモカイン / シグナル伝達経路 / バイオフィルム / アドレノメデュリン / 抗菌ペプチド / 齲蝕 / ミニマムインターベンション / 高周波/電磁波 / アンモニウムヘクサフルオロシリケート / 齲蝕治療法 / ミニマルインターベンション / 象牙質再石灰化法 / 電磁波/高周波 / 自然免疫応答 / ミニマルインターベンション（MI） / フッ化ジアミンシリケート / ポリフェノール / 歯周炎 / 線維芽細胞 / 上皮細胞 / クローディン / オクルーディン / シグナル伝達 / 共培養 / 自然免疫 / 象牙芽細胞 / 免疫応答 / Alarmin / 歯髄 / 炎症 / リンパ球 / クオラムセンシング / オートインデューサー / 深在性齲蝕 / 自然免疫機構 / 高周波/電磁波治療 / 電磁波・高周波 / Streptococcus mutans / 殺菌効果 / 骨芽細胞 / 成長因子 / 歯周病関連細菌 / in situPCR / RT-PCR / 接合上皮 / IL-8 / ICAM-1 / MAPキナーゼ / in situ PCR / in situ / 免疫染色 / 歯周ポケット / 侵入 / Periodontopathic bacteria / in situ-PCR / Junctional epithelium / MAP kinase / 根尖性歯周炎 / 根尖孔外 / 細菌 / 歯根部象牙質 / 免疫組織 / 走査型電子顕微鏡 / 難治性根尖性歯周炎 / 根管治療 / 感染根管 / 根尖孔 / apical periodontitis / extra-radical area / bacteria / root dentin / immunohistology / SEM / refractory apical periodontitis / infected root canal treatment / 象牙質齲蝕 / 16S rDNA / 電顕用樹脂 / TEM-in situ hybridization / Toll-like receptor / Dentinal caries / Embedding material for TEM / 難治症例 / 遺伝子増幅 / 細菌感染 / 特異的遺伝子配列 / Periapical Periodontitis / Refractory case / Gene Amplification / Bacterial Infection / Specific Gene Sequence / tight junction / adherence junction / claudin / cadherin / JAM-A / MMP / タイトジャンクション / tight-junction / トランスフェクション / fight iunction / epithelial cell / periodontal disease / Th17 / IL-17 / 石灰化 / VEGF / CAPE / KN-3 / マイクロエンドモデル / ラット / 免疫組織化学 / 電磁波 / 高周波根尖療法 / 創傷の治癒 / マイクロCT / リアルタイムPCR / 感染根管治療 / 電磁波照射 / EMAT / 病理組織学的観察 / ﾘｱﾙﾀｲﾑPCR / 銀ナノ粒子 / う蝕治療 / 初期歯髄炎 / 在宅・訪問診療 / 深在性う蝕 / 訪問・在宅治療 / 在宅・訪問歯科治 / 齲蝕治療 / 健康寿命 / 在宅・訪問歯科治療 / 慢性腎臓病 / ペリオドンタルメディシン / 歯周病原細菌 / 血清抗体価 / 推算糸球体濾過量 / 二酸化チタン / チタニアナノチューブ / 光触媒 / 看護支援プログラム / 糖尿病看護 / 口腔保健行動 / 効果検証 / 教育教材 / 糖尿病患者 / エクソソーム / 糖尿病関連歯周炎 / 高グルコース / 歯周医学 / 炎症反応 / AGEs / 酸化ストレス / 全身炎症性疾患 / 糖尿病 / セルフケア支援 / 看護 / プログラム / ナノカプセル / キトサン

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2020年11月27日

糖尿病を有する妊婦に看護職が行う簡易口腔保健支援プログラムの開発：慢性炎症性疾患である歯周病の回避と周産期医療の向上に向けて

桑村由美岸田佐智安井敏之遠藤逸朗吉田守美子松久宗英苛原稔湯本浩通坂本英次郎日野出大輔

2021年11月18日

歯周病の原因菌であるフゾバクテリウムが口腔がんの進展を促進する

工藤保誠藤原奈津美石丸直澄尾崎和美湯本浩通吉田賀弥

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湯本浩通