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向井理恵

徳島大学

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KAKEN
注目研究

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2024年11月21日更新

職名: 准教授
電話: 088-656-9917
電子メール: rmukai@tokushima-u.ac.jp
学歴: 2000/3: 東京都立短期大学・健康栄養学科卒業
2002/4: 神戸大学・農学部・生物機能化学科編入学
2004/3: 神戸大学・農学部・生物機能化学科卒業
2004/4: 神戸大学大学院自然科学研究科生物機能化学専攻(前期課程) 入学
2006/3: 神戸大学大学院自然科学研究科生物機能化学専攻(前期課程) 修了
2006/4: 神戸大学大学院自然科学研究科生命機構科学専攻(後期課程) 進学
2009/3: 神戸大学大学院自然科学研究科生命機構科学専攻(後期課程) 修了
学位: 博士(農学) (神戸大学) (2009年3月)
職歴・経歴: 2000/4: 株式会社ニチレイ
2006/4: 神戸市立工業高等専門学校，非常勤講師
2006/4: 独立行政法人日本学術振興会，特別研究員
2009/4: 国立大学法人徳島大学ヘルスバイオサイエンス研究部，食品機能学分野，学術研究員
2011/4: 国立大学法人徳島大学ヘルスバイオサイエンス研究部，食品機能学分野，助教
2016/4: 徳島大学大学院生物資源産業学研究科，食料科学分野，講師
2017/10: 徳島大学大学院生物資源産業学研究科，食料科学分野，准教授

専門分野・研究分野: 食品科学 (Food Science)
栄養化学 (Nutritional Chemistry)
機能性食品

研究者総覧で最新データを確認する

2024年11月21日更新

専門分野・研究分野: 食品科学 (Food Science)
栄養化学 (Nutritional Chemistry)
機能性食品
担当経験のある授業科目: フードサイエンス (学部)
卒業研究 (学部)
基礎食品化学 (学部)
機能性食品学特論 (大学院)
機能食品学 (学部)
生物資源学研究 (大学院)
生物資源産業学B (学部)
生物資源産業学実習 (学部)
生物資源産業学専門英語 (学部)
英語論文講読 (学部)
英語論文講読Ⅰ (学部)
英語論文講読Ⅱ (学部)
食と健康概論 (学部)
食品・生物資源関連法規 (学部)
食品化学 (学部)
食品学Ⅰ (学部)
食品機能学特論 (大学院)
食料生物科学特別実習 (大学院)
食料生物科学特別演習 (大学院)
食料生物科学特別研究 (大学院)
食料生物科学特別講義 (大学院)
食料科学基礎実習 (学部)
食料科学実習A (学部)
食料科学実習B (学部)
食料科学実習C (学部)
食料科学実習Ⅰ (学部)
食料科学実習Ⅱ (学部)
食料科学概論 (学部)
指導経験: 17人 (学士), 15人 (修士), 1人 (博士)

研究者総覧で最新データを確認する

2024年11月21日更新

専門分野・研究分野: 食品科学 (Food Science)
栄養化学 (Nutritional Chemistry)
機能性食品

研究テーマ: 食品に含まれるフラボノイドの生理機能の研究
食品成分の体内動態と筋萎縮予防改善効果について
植物性食品の健康増進作用

著書

Y Bandaruk, Rie Mukai and Junji Terao :
Role of flavonoids as possible monoamine oxidase-A regulators in the model of serotoninergic neuroblastoma SH-SY5Y cell.,
Ed by K. Yoshida, Yujinsha, 向井理恵 :
骨格筋萎縮の予防,
朝倉書店, 日本, 2023年11月.

(キーワード): 骨格筋萎縮の予防

向井理恵 :
フラボノイドによる骨格筋萎縮予防,
インフォノーツパブリッシング, 2022年8月. 河井(近藤) あかり, 酒井徹, 寺尾純二, 向井理恵 :
論文紹介「血管内皮細胞における過酸化水素誘導性カベリオン‐1リン酸化にたいするケルセチンの抑制効果,
日本ビタミン学会, 2021年12月. 向井理恵 :
第4章ポリフェノール,
株式会社シーエムシー出版, 2020年5月. 向井理恵 :
高い活性が期待されるマメ由来プレニルフラボノイドの血管内皮保護作用,
飯島藤十郎記念食品科学振興財団, 2020年. 向井理恵 :
高活性プレニルフラボノイドの臓器蓄積特性の解明,
2019年. 向井理恵 :
セミナー室，「植物ポリフェノールによる筋萎縮予防の可能性」,
公益社団法人日本農芸化学会, 2016年10月. 丸山如江, 向井理恵, 吉永直子, 裏出令子 :
JST「日中女性科学者シンポジウム2016 in Japan」見聞録,
公益社団法人日本農芸学会, 2016年. 向井理恵, 寺尾純二 :
「筋萎縮予防因子としての食事性ポリフェノール」,
株式会社建帛社, 2015年5月. 向井理恵, 寺尾純二 :
プレニル化はフラボノイドの機能性を増強する,
一般財団法人バイオインダストリー協会, 2015年5月. 篠田粧子 (名), 永吉道子 (名), 稲山貴代 (名), 大山珠美 (名), 岡本裕子 (名), 笠原利英 (名), 小林実夏 (名), 酒井徹 (名), 関口紀子 (名), 高森恵美子 (名), 田草川憲男 (名), 宮沢栄次 (名), 向井理恵, 吉田勉 (名) :
国民健康・栄養緒現状と問題点,
三共出版株式会社, 2015年3月. 向井理恵, 寺尾純二 :
今日の話題，プレニルフラボノイドの生体利用性「プレニル化は体内滞留時間を延長させて，組織への蓄積を高める」,
日本農芸化学会会誌, 2015年1月. Rie Mukai, N Matsui, N Matsumoto, DX Hou, T Nikawa, H Nemoto and J Terao :
Anti-Oxidative Flavonoid suppresses disuse muscle atrophy in denervation mice,
Ed by K. Yoshida, Yujinsha, 2014. Junji Terao, Rie Mukai, Yamashita Y., Ochi Arisa, Kohno Shohei, Katsuya Hirasaka and Takeshi Nikawa :
Chapter 7. Food Functions Preventing Muscle Atrophy.,
2014. 向井理恵, 二川健, 寺尾純二 :
フラボノイドによる廃用性筋萎縮予防,
株式会社シーエムシー出版, 2013年10月. 向井理恵, 根本尚夫, 二川健, 寺尾純二 :
「プレニルフラボノイドの生理機能性」,
日本ビタミン学会, 2013年2月.

(キーワード): Prenylated flavonoids / Disuse muscle atrophy / Sarcopenia / Ubiquitin ligase / Isoprenoid
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205669531264

(CiNii: 1390001205669531264) Rie Mukai, T. Nikawa and J. Terao :
Antioxidative flavonoid quercetin suppresses disused muscle atrophy,
London(United Kingdom), Sep. 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.freeradbiomed.2012.08.181
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.freeradbiomed.2012.08.181

(DOI: 10.1016/j.freeradbiomed.2012.08.181) 向井理恵, 寺尾純二 :
「イソフラボンと抗酸化活性評価」,
フジメディカル出版, 2011年10月. 向井理恵, 寺尾純二 :
Functional Food 機能性食品の基礎から臨床へ (吉川敏一編集), --- 〈第1特集〉5.イソフラボンと抗酸化活性評価 ---,
フジメディカル出版, 大阪, 2011年10月. Rie Mukai, Junji Terao, Yasuhito Shirai, Naoaki Saito and Hitoshi Ashida :
Determination of Subcellular Localization of Flavonol in Cultured Cells by Laser Scanning. (Edited by: Chau-Chang Wang), --- Laser Scanning, Theory and Applications Chapter12 ---,
InTech, Apr. 2011.

(出版サイトへのリンク): ● Publication site (DOI): 10.5772/15717
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.5772/15717

(DOI: 10.5772/15717)

論文

Retsuo Kawakami, Rie Mukai, Matsumura Takumi, Fujii Haruki, Jinbo Kurumi, Sogawa Ryutaro, Hashimura Nene and Ohashi Koichi :
Incremental effects of near-atmospheric-pressure low-temperature air plasma jet irradiation on polyphenol content in harvested onions,
Journal of Physics D: Applied Physics, Vol.57, 475201:1-475201:11, 2024.

(徳島大学機関リポジトリ): ● Metadata: 119603
(出版サイトへのリンク): ● Publication site (DOI): 10.1088/1361-6463/ad71db
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1088/1361-6463/ad71db

(徳島大学機関リポジトリ: 119603, DOI: 10.1088/1361-6463/ad71db) Rie Mukai, Hitomi Okuyama, Miku Uchimura, Kozue Sakao, Miyu Matsuhiro, Mayumi Ikeda-Imafuku, Yu Ishima, Miyu Nishikawa, Shinichi Ikushiro and Akihiro Tai :
The binding selectivity of quercetin and its structure-related polyphenols to human serum albumin using a fluorescent dye cocktail for multiplex drug-site mapping.,
Bioorganic Chemistry, Vol.145, 107184, 2024.

(要約): Human serum albumin (HSA) is a serum protein that carries flavonoids in blood circulation. In this report, the binding selectivity and strength of interactions to HSA-binding sites (sites I or II) by flavonoids were evaluated using competition experiments and the specific fluorescent dyes, dansylamide and BD140. Most tested flavonoids bound site I preferentially, with the binding strength dependent on the mother structure in the order flavonol > flavone > flavanone > flavan 3-ols. Glycosylation or glucuronidation reduced the binding of quercetin to site I of HSA, whereas sulfation increased binding. Quercetin 7-sulfate showed the strongest binding and molecular docking simulations supported this observation. Prenylation at any position or glucuronidation and sulfation at the C-4' or C-7 position of quercetin facilitated stronger binding to site II. The binding affinity of flavonoids toward site I correlated with the partition coefficient value (logP), whereas no corresponding correlation was observed for site II.
(キーワード): Humans / Serum Albumin, Human / Quercetin / Polyphenols / Fluorescent Dyes / Molecular Docking Simulation / Flavonoids / Binding Sites / Protein Binding / Spectrometry, Fluorescence
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bioorg.2024.107184
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38364549; ● Search Scopus @ Elsevier (PMID): 38364549; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bioorg.2024.107184

(DOI: 10.1016/j.bioorg.2024.107184, PubMed: 38364549) Rie Mukai and Natsumi Hata :
Tissue distribution and pharmacokinetics of isoxanthohumol from hops in rodents,
Food Science & Nutrition, Vol.12, No.3, 2210-2219, 2023.

(キーワード): Rie Mukai / Natsumi Hata
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/fsn3.3900
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1002/fsn3.3900

(DOI: 10.1002/fsn3.3900) Nao Fujiwara, Rie Mukai, Miyu Nishikawa, Shinichi Ikushiro, Akira Murakami and Akari Ishisaka :
Transfer of quercetin ingested by maternal mice to neonatal mice via breast milk.,
Bioscience, Biotechnology, and Biochemistry, Vol.87, No.4, 442-447, 2023.

(要約): This is the first study that quantified quercetin (QUE) and its 16 metabolites in the breast milk of QUE-fed maternal mice, the plasma and urine of that, and neonatal mice. Interestingly, the QUE aglycone concentration in the milk was much higher than in the plasma of maternal mice, suggesting that QUE may exert biological activity in neonates.
(キーワード): Animals / Mice / Quercetin / Milk, Human / Animals, Newborn
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/bbb/zbad007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36669760; ● Summary page in Scopus @ Elsevier: 2-s2.0-85150751148

(DOI: 10.1093/bbb/zbad007, PubMed: 36669760, Elsevier: Scopus) Nuka Erika, Takahashi Masako, Okitsu Masami, Nayama Chisako, Nishijima Honomi, Sogawa Ryutaro, Kawabata Kyuichi, Terao Junji and Rie Mukai :
Lowering effect of combined sweet potato and onion intake on plasma quercetin concentration and underlying mechanism involving intestinal β-glucosidase activity.,
Bioscience, Biotechnology, and Biochemistry, Vol.86, No.12, 1695-1698, 2022.

(要約): A combined intake of cooked sweet potato and fried onion in humans was found to suppress the increase of plasma quercetin metabolite concentration. Experiments using rat β-glucosidase indicated that excess carbohydrate digestion products, especially glucose-containing saccharides, interfere with the deglycosylation of quercetin glucosides during intestinal epithelial uptake. Combined meals of sweet potato and onion may lower the bioavailability of onion quercetin glucosides.
(キーワード): Humans / Rats / Animals / Onions / Quercetin / Ipomoea batatas / Glucosides / beta-Glucosidase
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/bbb/zbac155
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36102655; ● Summary page in Scopus @ Elsevier: 2-s2.0-85142918919

(DOI: 10.1093/bbb/zbac155, PubMed: 36102655, Elsevier: Scopus) Yoshiaki Tanaka, Hitomi Okuyama, Miyu Nishikawa, Shin-ichi Ikushiro, Mayumi Ikeda, Yu Ishima, Yuichi Ukawa, Kenichi Oe, Junji Terao and Rie Mukai :
8-Prenylnaringenin tissue distribution and pharmacokinetics in mice and its binding to human serum albumin and cellular uptake in human embryonic kidney cells.,
Food Science & Nutrition, Vol.10, No.4, 1070-1080, 2022.

(要約): 8-Prenylnaringenin (8-PN), a hop flavonoid, is a promising food substance with health benefits. Compared with nonprenylated naringenin, 8-PN exhibits stronger estrogenic activity and prevents muscle atrophy. Moreover, 8-PN prevents hot flushes and bone loss. Considering that prenylation reportedly improves the bioavailability of flavonoids, we compared the parameters related to the bioavailability [pharmacokinetics and tissue distribution in C57/BL6 mice, binding affinity to human serum albumin (HSA), and cellular uptake in HEK293 cells] of 8-PN and its mother (non-prenylated) compound naringenin. C57/BL6 mice were fed an 8-PN or naringenin mixed diet for 22 days. The amount of 8-PN (nmol/g tissue) in the kidneys (16.8 ± 9.20), liver (14.8 ± 2.58), muscles (3.33 ± 0.60), lungs (2.07 ± 0.68), pancreas (1.80 ± 0.38), heart (1.71 ± 0.27), spleen (1.36 ± 0.29), and brain (0.31 ± 0.09) was higher than that of naringenin. A pharmacokinetic study in mice demonstrated that the of 8-PN (50 mg/kg body weight) was lower than that of naringenin; however, the plasma concentration of 8-PN 8 h after ingestion was higher than that of naringenin. The binding affinity of 8-PN to HSA and cellular uptake in HEK293 cells were higher than those of naringenin. 8-PN bioavailability features assessed in mouse or human model experiments were obviously different from those of naringenin.
(徳島大学機関リポジトリ): ● Metadata: 117445
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/fsn3.2733
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35432956; ● Summary page in Scopus @ Elsevier: 2-s2.0-85123466355

(徳島大学機関リポジトリ: 117445, DOI: 10.1002/fsn3.2733, PubMed: 35432956, Elsevier: Scopus) Takahiro Takase, Satoshi Ikeuchi, Takashi Inoue and Rie Mukai :
Eriocitrin Contained in Lemon Peel Ameliorates Disuse Muscle Atrophy by Suppressing the Expression of Atrogin-1 and MuRF-1 in Denervated Mice.,
Journal of Natural Products, Vol.84, No.7, 2048-2052, 2021.

(要約): Unloading stress enhances oxidative stress, which in turn induces disuse muscle atrophy. This study evaluated the suppressive effect of lemon peel extract containing eriocitrin on muscle atrophy. Both lemon peel extract and eriocitrin suppressed weight loss in the gastrocnemius muscle under denervation in C57BL/6 mice. The mRNA level of ubiquitin ligases and their transcription factor were downregulated by eriocitrin. Eriocitrin inhibited the increase in lipid peroxidation and the ratio of glutathione disulfide/glutathione. These data suggest that eriocitrin ameliorated disuse muscle atrophy by suppressing the expression of ubiquitin ligase genes by its antioxidative effect.
(キーワード): Animals / Citrus / Flavanones / Fruit / Lipid Peroxidation / Male / Mice, Inbred C57BL / Molecular Structure / Muscle Denervation / Muscle Proteins / Muscle, Skeletal / Muscular Atrophy / Oxidative Stress / Plant Extracts / SKP Cullin F-Box Protein Ligases
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/acs.jnatprod.1c00271
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34189920; ● Search Scopus @ Elsevier (PMID): 34189920; ● Search Scopus @ Elsevier (DOI): 10.1021/acs.jnatprod.1c00271

(DOI: 10.1021/acs.jnatprod.1c00271, PubMed: 34189920) Akari Kondo-Kawai, Tohru Sakai, Junji Terao and Rie Mukai :
Suppressive effects of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in endothelial cells,
Journal of Clinical Biochemistry and Nutrition, Vol.69, No.1, 1-9, 2021.

(要約): Caveolin-1 is a major protein of the caveolae structure in vascular endothelial cell membrane. Phosphorylation of caveolin-1 is one of the initial events leading to exacerbation of vascular permeability caused by oxidative stress. Although quercetin is known to be an anti-atherosclerosis factor that acts as a dietary antioxidant, little is known about its role in the regulation of caveolin-1 phosphorylation. In this study, we investigated the inhibitory effect of quercetin on hydrogen peroxide-induced caveolin-1 phosphorylation in human umbilical vein endothelial cells. Quercetin inhibited caveolin-1 phosphorylation in cells pretreated with quercetin for 24 h and then exposed to hydrogen peroxide. However, quercetin 3--β-glucuronide, a conjugated metabolite of quercetin, did not exert this inhibitory effect. Exposure to hydrogen peroxide increased vascular permeability and reduced mRNA expression of the intercellular adhesion protein, vascular endothelial cadherin (VE-cadherin). By contrast, pretreatment with quercetin suppressed the increase in vascular permeability and decreased VE-cadherin expression. These results indicate that deconjugated quercetin can play a role in the prevention of altered vascular permeability under oxidative stress by suppressing caveolin-1 phosphorylation. Thus, dietary quercetin may be beneficial for the maintenance of endothelial cell function.
(徳島大学機関リポジトリ): ● Metadata: 116063
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.20-190
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34376911; ● Search Scopus @ Elsevier (PMID): 34376911; ● Search Scopus @ Elsevier (DOI): 10.3164/jcbn.20-190

(徳島大学機関リポジトリ: 116063, DOI: 10.3164/jcbn.20-190, PubMed: 34376911) Rie Mukai, Takashi Fukuda, Asami Ohnishi, Takeshi Nikawa, Mutsuki Furusawa and Junji Terao :
Chocolate as a food matrix reduces the bioavailability of galloylated catechins from green tea in healthy women.,
Food & Function, Vol.12, No.1, 408-416, 2021.

(要約): In this study, we evaluated the food matrix effects of chocolate on the absorption of green tea catechins (GTCs), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg), and (-)-epigallocatechin gallate (EGCg), in five healthy 22-year-old women. In the single-intake experiment, the plasma concentrations of ECg (P < 0.05, at 1.5 h) and EGCg (P < 0.05, at 6 h) but not those of EC and EGC were reduced by the chocolate matrix. Regardless of the chocolate matrix, ECg and EGCg were mainly present as their aglycones in the plasma, whereas EGC and EC were found mostly as conjugated metabolites. After daily intake of GTCs mixed with chocolate for 14 days followed by overnight fasting, ECg but not EGCg was detected in the plasma. To compare the plasma profiles of ECg and EGCg, a mixture containing approximately equal amounts of ECg and EGCg was administered to nine rats for 14 days. Following treatment and overnight food deprivation, the plasma content of ECg was higher than that of EGCg. After a single injection of the same mixture in seven rats, ECg levels were higher than those of EGCg, and a greater amount of conjugated metabolites of ECg than those of EGCg was detected in the plasma 10 h after administration. In conclusion, the chocolate matrix affects the plasma profiles of GTCs, particularly ECg. ECg appears to persist in the plasma for a longer period, regardless of the chocolate matrix.
(キーワード): Adult / Animals / Biological Availability / Catechin / Chocolate / Female / Food / Humans / Male / Models, Animal / Rats / Rats, Wistar / Reference Values / Tea / Young Adult
(徳島大学機関リポジトリ): ● Metadata: 115529
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/d0fo02485f
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33393957; ● Search Scopus @ Elsevier (PMID): 33393957; ● Search Scopus @ Elsevier (DOI): 10.1039/d0fo02485f

(徳島大学機関リポジトリ: 115529, DOI: 10.1039/d0fo02485f, PubMed: 33393957) Agustin Martin Morales, Rie Mukai, Kaeko Murota and Junji Terao :
Inhibitory effect of catecholic colonic metabolites of rutin on fatty acid hydroperoxide and hemoglobin dependent lipid peroxidation in Caco-2 cells.,
Journal of Clinical Biochemistry and Nutrition, Vol.63, No.3, 175-180, 2018.

(要約): To determine the preventive effect of dietary rutin on oxidative damages occurring in the digestive tract, 13-hydroperoxyoctadecadienoic acid and hemoglobin were exposed to Caco-2 intestinal cells after the pretreatment with colonic rutin metabolites. Among four catechol-type metabolites, quercetin and 3,4-dihydroxytoluene exerted significant protection on 13-hydroperoxyoctadecadienoic and hemoglobin-dependent lipid peroxidation of this epithelial cell. Compared with quercetin, a much lower concentration allowed 3,4-dihydroxytoluene to maximize the protective effect, though it needed a longer pre-incubation period. Neither quercetin nor 3,4-dihydroxytoluene affected the expression of peroxiredoxin-6 protein, which comprises the cellular antioxidant defense system. It is concluded that 3,4-dihydroxytoluene is a plausible rutin colonic metabolite that can suppress oxidative damages of intestinal epithelial cells by directly inhibiting lipid peroxidation. This result may illuminate the preventive role of dietary rutin against colorectal cancer incidence in relation to the consumption of red and processed meat.
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.18-38
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30487666; ● Summary page in Scopus @ Elsevier: 2-s2.0-85056139972

(DOI: 10.3164/jcbn.18-38, PubMed: 30487666, Elsevier: Scopus) Rie Mukai, Hitomi Horikawa, Pei-Yi Lin, Nao Tsukumo, Takeshi Nikawa, Tomoyuki Kawamura, Hisao Nemoto and Junji Terao :
8-Prenylnaringenin promotes recovery from immobilization-induced disuse muscle atrophy through activation of the Akt phosphorylation pathway in mice.,
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, Vol.311, No.6, R1022-R1031, 2016.

(要約): 8-Prenylnaringenin (8-PN) is a prenylflavonoid that originates from hop extracts and is thought to help prevent disuse muscle atrophy. We hypothesized that 8-PN affects muscle plasticity by promoting muscle recovery under disuse muscle atrophy. To test the promoting effect of 8-PN on muscle recovery, we administered an 8-PN mixed diet to mice that had been immobilized with a cast to one leg for 14 days. Intake of the 8-PN mixed diet accelerated recovery from muscle atrophy, and prevented reductions in Akt phosphorylation. Studies on cell cultures of mouse myotubes in vitro demonstrated that 8-PN activated the PI3K/Akt/P70S6K1 pathway at physiological concentrations. A cell-culture study using an inhibitor of estrogen receptors and an in vivo experiment with ovariectomized mice suggested that the estrogenic activity of 8-PN contributed to recovery from disuse muscle atrophy through activation of an Akt phosphorylation pathway. These data strongly suggest that 8-PN is a naturally occurring compound that could be used as a nutritional supplement to aid recovery from disuse muscle atrophy.
(キーワード): Animals / Cell Line / Enzyme Activation / Flavanones / Hindlimb Suspension / Male / Mice / Mice, Inbred C57BL / Muscle Proteins / Muscle, Skeletal / Muscular Atrophy / Oncogene Protein v-akt / リン酸化 (phosphorylation) / Phytoestrogens / Protein Biosynthesis / Recovery of Function / シグナル伝達 (signal transduction) / Treatment Outcome
(徳島大学機関リポジトリ): ● Metadata: 112458
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpregu.00521.2015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27629889; ● Search Scopus @ Elsevier (PMID): 27629889; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpregu.00521.2015

(徳島大学機関リポジトリ: 112458, DOI: 10.1152/ajpregu.00521.2015, PubMed: 27629889) Chiemi Kamada, Rie Mukai, Akari Kondo, Shinya Sato and Junji Terao :
Effect of quercetin and its metabolite on caveolin-1 expression induced by oxidized LDL and lysophosphatidylcholine in endothelial cells.,
Journal of Clinical Biochemistry and Nutrition, Vol.58, No.3, 193-201, 2016.

(要約): Oxidized low-density lipoprotein contributes to atherosclerotic plaque formation, and quercetin is expected to exert anti-atherosclerotic effects. We previously reported accumulation of conjugated quercetin metabolites in the aorta of rabbits fed high-cholesterol diets with quercetin glucosides, resulting in attenuation of lipid peroxidation and inhibition of lipid accumulation. Caveolin-1, a major structural protein of caveolae in vascular endothelial cells, plays a role in atherosclerosis development. Here we investigated effects of oxidized low-density lipoprotein, quercetin and its metabolite, quercetin 3-O--glucuronide, on caveolin-1 expression. Oxidized low-density lipoprotein significantly upregulated caveolin-1 mRNA expression. An oxidized low-density lipoprotein component, lysophosphatidylcholine, also induced expression of both caveolin-1 mRNA and protein. However, lysophosphatidylcholine did not affect the location of caveolin-1 proteins within caveolae structures. Co-treatment with quercetin or quercetin 3-O--glucuronide inhibited lysophosphatidylcholine-induced caveolin-1 expression. Quercetin and quercetin 3-O--glucuronide also suppressed expression of adhesion molecules induced by oxidized low-density lipoprotein and lysophosphatidylcholine. These results strongly suggest lysophosphatidylcholine derived from oxidized low-density lipoprotein contributes to atherosclerotic events by upregulating caveolin-1 expression, resulting in induction of adhesion molecules. Quercetin metabolites are likely to exert an anti-atherosclerotic effect by attenuating caveolin-1 expression in endothelial cells.
(徳島大学機関リポジトリ): ● Metadata: 109709
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.16-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27257344; ● Search Scopus @ Elsevier (PMID): 27257344; ● Search Scopus @ Elsevier (DOI): 10.3164/jcbn.16-2

(徳島大学機関リポジトリ: 109709, DOI: 10.3164/jcbn.16-2, PubMed: 27257344) Ayami Hisanaga, Rie Mukai, Kozue Sakao, Junji Terao and De-Xing Hou :
Anti-inflammatory effects and molecular mechanisms of 8-prenyl quercetin.,
Molecular Nutrition & Food Research, Vol.60, No.5, 1020-1032, 2016.

(要約): PQ as a potential inhibitor revealed anti-inflammatory effect in both cell and animal models at least by targeting SEK1-JNK1/2 and MEK1-ERK1/2.
(徳島大学機関リポジトリ): ● Metadata: 112470
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/mnfr.201500871
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26872410; ● Search Scopus @ Elsevier (PMID): 26872410; ● Search Scopus @ Elsevier (DOI): 10.1002/mnfr.201500871

(徳島大学機関リポジトリ: 112470, DOI: 10.1002/mnfr.201500871, PubMed: 26872410) Rie Mukai, Naoko Matsui, Yutaka Fujikura, Norifumi Matsumoto, De-Xing Hou, Noriyuki Kanzaki, Hiroshi Shibata, Manabu Horikawa, Keiko Iwasa, Katsuya Hirasaka, Takeshi Nikawa and Junji Terao :
Preventive effect of dietary quercetin on disuse muscle atrophy by targeting mitochondria in denervated mice.,
The Journal of Nutritional Biochemistry, Vol.31, 67-76, 2016.

(要約): Quercetin is a major dietary flavonoid in fruits and vegetables. We aimed to clarify the preventive effect of dietary quercetin on disuse muscle atrophy and the underlying mechanisms. We established a mouse denervation model by cutting the sciatic nerve in the right leg (SNX surgery) to lack of mobilization in hind-limb. Preintake of a quercetin-mixed diet for 14days before SNX surgery prevented loss of muscle mass and atrophy of muscle fibers in the gastrocnemius muscle (GM). Phosphorylation of Akt, a key phosphorylation pathway of suppression of protein degradation, was activated in the quercetin-mixed diet group with and without SNX surgery. Intake of a quercetin-mixed diet suppressed the generation of hydrogen peroxide originating from mitochondria and elevated mitochondrial peroxisome proliferator-activated receptor- coactivator 1 mRNA expression as well as NADH dehydrogenase 4 expression in the GM with SNX surgery. Quercetin and its conjugated metabolites reduced hydrogen peroxide production in the mitochondrial fraction obtained from atrophied muscle. In C2C12 myotubes, quercetin reached the mitochondrial fraction. These findings suggest that dietary quercetin can prevent disuse muscle atrophy by targeting mitochondria in skeletal muscle tissue through protecting mitochondria from decreased biogenesis and reducing mitochondrial hydrogen peroxide release, which can be related to decreased hydrogen peroxide production and/or improvements on antioxidant capacity of mitochondria.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jnutbio.2016.02.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27133425; ● Search Scopus @ Elsevier (PMID): 27133425; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jnutbio.2016.02.001

(DOI: 10.1016/j.jnutbio.2016.02.001, PubMed: 27133425) Yasuaki Kashino, Kaeko Murota, Namiko Matsuda, Muneaki Tomotake, Takuya Hamano, Rie Mukai and Junji Terao :
Effect of Processed Onions on the Plasma Concentration of Quercetin in Rats and Humans.,
Journal of Food Science, Vol.80, No.11, H2597-H2602, 2015.

(要約): Onion is a popular source of antioxidative flavonoid quercetin and its vascular function attracts considerable attention in relation to anti-atherosclerotic effect. The present study estimated the effect of food processing on the bioavailability of onion quercetin aglycone and its glucosides provided through the consumption of onion products. The intake of a peel powder-containing meal showed a significantly higher bioavailability than the peel extract, bulb powder, bulb extract, and bulb sauté containing meals. Hence, food processing of onion peel may enhance the health impact of onion quercetin by elevating its bioavailability.
(キーワード): Adult / Animals / Biological Availability / Diet / Female / Food Handling / Glucosides / Humans / Male / Onions / Plant Epidermis / Plant Preparations / Plant Roots / Quercetin / Rats, Wistar / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/1750-3841.13079
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26375490; ● Search Scopus @ Elsevier (PMID): 26375490; ● Search Scopus @ Elsevier (DOI): 10.1111/1750-3841.13079

(DOI: 10.1111/1750-3841.13079, PubMed: 26375490) Manabu Ueda-Wakagi, Rie Mukai, Naoya Fuse, Yoshiyuki Mizushina and Hitoshi Ashida :
3-O-Acyl-epicatechins Increase Glucose Uptake Activity and GLUT4 Translocation through Activation of PI3K Signaling in Skeletal Muscle Cells.,
International Journal of Molecular Sciences, Vol.16, No.7, 16288-16299, 2015.

(要約): Tea catechins promote glucose uptake in skeletal muscle cells. In this study, we investigated whether the addition of an acyl group to the C-3 position of catechins to generate 3-O-acyl-catechins promoted glucose uptake in L6 myotubes. 3-O-Myristoyl-(-)-epicatechin (EC-C14) and 3-O-palmitoyl-(-)-epicatechin (EC-C16) promoted glucose uptake and translocation of glucose transporter (GLUT) 4 in the cells. The effect of 3-O-acyl-(-)-epicatechins was stronger than that of (-)-epicatechin (EC), whereas neither 3-O-myristoyl-(+)-catechin (C-C14) nor 3-O-palmitoyl-(+)catechin (C-C16) promoted glucose uptake or GLUT4 translocation as well as (+)-catechin (C). We further investigated an affinity of catechins and 3-O-acyl-catechins to the lipid bilayer membrane by using surface plasma resonance analysis. Maximum binding amounts of EC-C16 and C-C16 to the lipid bilayer clearly increased compared with that of (-)-EC and (+)-C, respectively. We also examined the mechanism of GLUT4 translocation and found EC-C14 and EC-C16 induced the phosphorylation of PI3K, but did not affect phosphorylation of Akt or IR. In conclusion, the addition of an acyl group to the C-3 position of (-)-EC increases its affinity for the lipid bilayer membrane and promotes GLUT4 translocation through PI3K-dependent pathways in L6 myotubes.
(キーワード): Animals / Catechin / Glucose / Glucose Transporter Type 4 / Insulin / Lipid Bilayers / Muscle Cells / Muscle Fibers, Skeletal / Muscle, Skeletal / Phosphatidylinositol 3-Kinases / Protein Transport / Rats / Signal Transduction
(徳島大学機関リポジトリ): ● Metadata: 113569
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms160716288
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26193264; ● Search Scopus @ Elsevier (PMID): 26193264; ● Search Scopus @ Elsevier (DOI): 10.3390/ijms160716288

(徳島大学機関リポジトリ: 113569, DOI: 10.3390/ijms160716288, PubMed: 26193264) Rita Cristina Orihuela Campos, Naofumi Tamaki, Rie Mukai, Makoto Fukui, Kaname Miki, Junji Terao and Hiro-O Ito :
Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts.,
Journal of Clinical Biochemistry and Nutrition, Vol.56, No.3, 220-227, 2015.

(要約): In periodontitis, production of reactive oxygen species (ROS) by neutrophils induces oxidative stress and deteriorates surrounding tissues. Antioxidants reduce damage caused by ROS and are used to treat diseases involving oxidative stress. This study summarizes the different effects of resveratrol, quercetin, and N-acetylcysteine (NAC) on human gingival fibroblasts (HGFs) under oxidative stress induced by hydrogen peroxide. Real-time cytotoxicity analyses reveals that resveratrol and quercetin enhanced cell proliferation even under oxidative stress. Of the antioxidants tested, resveratrol is the most effective at inhibiting ROS production. HGFs incubated with resveratrol and quercetin up-regulate the transcription of type I collagen gene after 3 h, but only resveratrol sustained this up-regulation for 24 h. A measurement of the oxygen consumption rate (OCR, mitochondrial respiration) shows that resveratrol generates the highest maximal respiratory capacity, followed by quercetin and NAC. Simultaneous measurement of OCR and the extracellular acidification rate (non-mitochondrial respiration) reveals that resveratrol and quercetin induce an increase in mitochondrial respiration when compared with untreated cells. NAC treatment consumes less oxygen and enhances more non-mitochondrial respiration. In conclusion, resveratrol is the most effective antioxidant in terms of real-time cytotoxicity analysis, reduction of ROS production, and enhancement of type I collagen synthesis and mitochondrial respiration in HGFs.
(徳島大学機関リポジトリ): ● Metadata: 109399
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.14-129
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26060353; ● Summary page in Scopus @ Elsevier: 2-s2.0-84929618900

(徳島大学機関リポジトリ: 109399, DOI: 10.3164/jcbn.14-129, PubMed: 26060353, Elsevier: Scopus) Arisa Ochi, Tomoki Abe, Reiko Nakao, Yoriko Yamamoto, Kanako Kitahata, Marina Takagi, Katsuya Hirasaka, Ayako Ohno, Shigetada Teshima-Kondo, Gwag Taesik, Inho Choi, Tomoyuki Kawamura, Hisao Nemoto, Rie Mukai, Junji Terao and Takeshi Nikawa :
N-myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscle.,
Archives of Biochemistry and Biophysics, Vol.570, 23-31, 2015.

(要約): A DGpYMP peptide mimetic of tyrosine(608)-phosphorylated insulin receptor substrate-1 (IRS-1), named Cblin, was previously shown to significantly inhibit Cbl-b-mediated IRS-1 ubiquitination. In the present study, we developed N-myristoylated Cblin and investigated whether it was effective in preventing glucocorticoid-induced muscle atrophy. Using HEK293 cells overexpressing Cbl-b, IRS-1 and ubiquitin, we showed that the 50% inhibitory concentrations of Cbl-b-mediated IRS-1 ubiquitination by N-myristoylated Cblin and Cblin were 30 and 120M, respectively. Regarding the DEX-induced atrophy of C2C12 myotubes, N-myristoylated Cblin was more effective than Cblin for inhibiting the DEX-induced decreases in C2C12 myotube diameter and IRS-1 degradation. The inhibitory efficacy of N-myristoylated Cblin on IRS-1 ubiquitination in C2C12 myotubes was approximately fourfold larger than that of Cblin. Furthermore, N-myristoylation increased the incorporation of Cblin into HEK293 cells approximately 10-folds. Finally, we demonstrated that N-myristoylated Cblin prevented the wet weight loss, IRS-1 degradation, and MAFbx/atrogin-1 and MuRF-1 expression in gastrocnemius muscle of DEX-treated mice approximately fourfold more effectively than Cblin. Taken together, these results suggest that N-myristoylated Cblin prevents DEX-induced skeletal muscle atrophy in vitro and in vivo, and that N-myristoylated Cblin more effectively prevents muscle atrophy than unmodified Cblin.
(徳島大学機関リポジトリ): ● Metadata: 109380
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.abb.2015.02.006
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25689493; ● Summary page in Scopus @ Elsevier: 2-s2.0-84924084788

(徳島大学機関リポジトリ: 109380, DOI: 10.1016/j.abb.2015.02.006, PubMed: 25689493, Elsevier: Scopus) Itsuko Fukuda, Shin Nishiumi, Rie Mukai, Ken-ichi Yoshida and Hitoshi Ashida :
Catechins in tea suppress the activity of cytochrome P450 1A1 through the aryl hydrocarbon receptor activation pathway in rat livers.,
International Journal of Food Sciences and Nutrition, Vol.66, No.3, 300-307, 2015.

(要約): Polycyclic aromatic hydrocarbons (PAHs) and halogenated aromatic hydrocarbons (HAHs) develop various adverse effects through activation of an aryl hydrocarbon receptor (AhR). The suppressive effects of brewed green tea and black tea on 3-methylcholanthrene (MC)-induced AhR activation and its downstream events were examined in the liver of rats. Ad-libitum drinking of green tea and black tea suppressed MC-induced AhR activation and elevation of ethoxyresorufin O-deethylase activity in the liver, whereas the teas themselves did not induce them. Tea showed a suppressive fashion on the expression of cytochrome P450 1A1 (CYP1A1). Tea suppressed the AhR activation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) ex vivo. A part of catechins and theaflavins was present in plasma and liver as conjugated and intact forms. The results of this study suggested that active component(s) of tea are incorporated in the liver and suppress the activity of CYP1As through the AhR activation pathway.
(キーワード): Animals / Biflavonoids / Camellia sinensis / Catechin / Cytochrome P-450 CYP1A1 / Environmental Pollutants / Hydrocarbons, Halogenated / Liver / Methylcholanthrene / Plant Extracts / Polycyclic Hydrocarbons, Aromatic / Rats / Receptors, Aryl Hydrocarbon / Signal Transduction / Tea / Tetrachlorodibenzodioxin
(出版サイトへのリンク): ● Publication site (DOI): 10.3109/09637486.2014.992007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25582180; ● Search Scopus @ Elsevier (PMID): 25582180; ● Search Scopus @ Elsevier (DOI): 10.3109/09637486.2014.992007

(DOI: 10.3109/09637486.2014.992007, PubMed: 25582180) Rie Hashimoto, Atsuko Sakai, Masumi Murayama, Arisa Ochi, Tomoki Abe, Katsuya Hirasaka, Ayako Ohno, Shigetada Teshima-Kondo, Hiroaki Yanagawa, Natsuo Yasui, Mikiko Inatsugi, Daisuke Doi, Masanori Takeda, Rie Mukai, Junji Terao and Takeshi Nikawa :
Effects of dietary soy protein on skeletal muscle volume and strength in humans with various physical activities,
The Journal of Medical Investigation : JMI, Vol.62, No.3, 177-183, 2015.

(要約): Background: In recent years, the number of bedridden people is rapidly increasing due to aging or lack of exercise in Japan. This problem is becoming more serious, since there is no countermeasure against it. In the present study, we designed to investigate whether dietary proteins, especially soy, had beneficial effects on skeletal muscle in 59 volunteers with various physical activities. Methods: We subjected 59 volunteers with various physical activities to meal intervention examination. Persons with low and high physical activities were divided into two dietary groups, the casein diet group and the soy diet group. They ate daily meals supplemented with 7.8 g of powdered casein or soy protein isolate every day for 30 days. Bedridden patients in hospitals were further divided into three dietary groups: the no supplementation diet group, the casein diet group and the soy diet group. They were also subjected to a blood test, a urinalysis, magnetic resonance imaging analysis and muscle strength test of the knee before and after the meal intervention study. Results: Thirty-day soy protein supplementation significantly increased skeletal muscle volume in participants with low physical activity, compared with 30-day casein protein supplementation. Both casein and soy protein supplementation increased the volume of quadriceps femoris muscle in bedridden patients. Consistently, soy protein significantly increased their extension power of the knee, compared with casein protein. Although casein protein increased skeletal muscle volume more than soy protein in bedridden patients, their muscle strength changes by soy protein supplementation were bigger than those by casein protein supplementation. Conclusions: The supplementation of soy protein would be one of the effective foods which prevent the skeletal muscle atrophy caused by immobilization or unloading. J. Med. Invest. 62: 177-183, August, 2015
(徳島大学機関リポジトリ): ● Metadata: 109381
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.62.177
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26399344; ● CiNii @ 国立情報学研究所 (CRID): 1390282679220909952; ● Summary page in Scopus @ Elsevier: 2-s2.0-84942054092

(徳島大学機関リポジトリ: 109381, DOI: 10.2152/jmi.62.177, PubMed: 26399344, CiNii: 1390282679220909952, Elsevier: Scopus) 板東紀子, 片岡美樹, 中村俊之, 向井理恵, 山岸喬, 寺尾純二 :
大豆の加工形態によるイソフラボン生体吸収量の比較,
日本栄養・食糧学会誌, Vol.68, No.1, 25-29, 2015年.

(要約): 本試験は, 大豆イソフラボンの吸収性を大豆の加工形態の違いから比較した．5名の健常人男性が豆乳, 豆腐, 大豆煮豆, 納豆をそれぞれ単回摂取し, 摂取90分後の血中イソフラボン濃度の上昇値を吸収量とした．試験食中に含まれるダイゼイン, ゲニステイン, グリシテインとこれらの配糖体であるダイジン, ゲニスチン, グリシチンの合計をアグリコン換算で50 mg含むように摂取量を調整した．摂取前後の血中イソフラボンはアグリコンとして定量した．ダイゼイン, ゲニステインは試験食摂取後すべての被験者で上昇し, 摂取量に対する上昇値は納豆を除いてゲニステインがダイゼインより高い値であった．アグリコン換算した血漿中の総イソフラボン濃度の上昇は煮豆が豆腐に比べて大きい傾向を示した．以上の結果は大豆の加工形態がイソフラボンの生体吸収性に影響することを示唆するものである．
(キーワード): イソフラボン / 大豆加工食品 / 生体利用性 / ヒト試験
(出版サイトへのリンク): ● Publication site (DOI): 10.4327/jsnfs.68.25
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001206293822592; ● Search Scopus @ Elsevier (DOI): 10.4327/jsnfs.68.25

(DOI: 10.4327/jsnfs.68.25, CiNii: 1390001206293822592) Bandaruk Yauhen, Rie Mukai and Junji Terao :
Cellular uptake of quercetin and luteolin and their effects on monoamine oxidase-A in human neuroblastoma SH-SY5Y cells,
Toxicology Reports, Vol.1, No.1, 639-649, 2014.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.toxrep.2014.08.016
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.toxrep.2014.08.016

(DOI: 10.1016/j.toxrep.2014.08.016) Akari Ishisaka, Rie Mukai, Junji Terao, Noriyuki Shibata and Yoshichika Kawai :
Specific localization of quercetin-3-O-glucuronide in human brain.,
Archives of Biochemistry and Biophysics, Vol.557, 11-17, 2014.

(要約): In recent years, many papers have suggested that dietary flavonoids may exert beneficial effects in the brain tissue for the protection of neurons against oxidative stress and inflammation. However, the bioavailability of flavonoids across the blood-brain barrier and the localization in the brain remain controversial. Thus, we examined the localization of quercetin-3-O-glucuronide (Q3GA), a major phase-II metabolite of quercetin, in the human brain tissues with or without cerebral infarction by immunohistochemical staining using anti-Q3GA antibody. A significant immunoreactivity was observed in the epithelial cells of the choroid plexus, which constitute the structural basis of the blood-cerebrospinal fluid (CSF) barrier, and in the foamy macrophages of recent infarcts. The cellular accumulation of Q3GA was also reproduced in vitro in macrophage-like RAW264, microglial MG6, and brain capillary endothelial RBEC1. It is of interest that a common feature of these cell lines is the deconjugation of Q3GA, resulting in the cellular accumulation of non-conjugated quercetin and the methylated forms. We then examined the anti-inflammatory activity of Q3GA and the deconjugated forms in the lipopolysaccharide-stimulated macrophage cells and revealed that the deconjugated forms (quercetin and a methylated form isorhamnetin), but not Q3GA itself, exhibited inhibitory effects on the inflammatory responses through attenuation of the c-Jun N-terminal kinase pathway. These results suggested that a quercetin glucuronide can pass through the blood-brain barrier, perhaps the CSF barrier, accumulate in specific types of cells, such as macrophages, and act as anti-inflammatory agents in the brain through deconjugation into the bioactive non-conjugated forms.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.abb.2014.05.025
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24893148; ● Search Scopus @ Elsevier (PMID): 24893148; ● Search Scopus @ Elsevier (DOI): 10.1016/j.abb.2014.05.025

(DOI: 10.1016/j.abb.2014.05.025, PubMed: 24893148) Shinsuke Kido, Marina Fujihara, Kengo Nomura, Shohei Sasaki, Rie Mukai, Ritsuko Ohnishi, Ichiro Kaneko, Hiroko Segawa, Sawako Tatsumi, Hiroto Izumi, Kimitoshi Kohno and Ken-ichi Miyamoto :
Molecular mechanisms of cadmium-induced fibroblast growth factor 23 upregulation in osteoblast-like cells.,
Toxicological Sciences, Vol.139, No.2, 301-316, 2014.

(要約): Itai-itai disease is thought to be the result of chronic cadmium (Cd) intoxication. Renal proximal tubules are a major target of Cd toxicity. The whole mechanism of the adverse effects of Cd remains unresolved, especially how renal damage is related to the development of bone lesions. Fibroblast growth factor 23 (FGF23) is a bone-derived phosphaturic factor that regulates vitamin D and inorganic phosphate metabolism in the kidney. To clarify the role of FGF23 on Cd toxicity, we investigated the mechanisms of Cd-induced FGF23 production in the bone. Cd injection into mice significantly increased plasma FGF23 concentrations, but did not change FGF23 mRNA expression in bone. GalNAc-T3 is involved in secreting intact FGF23. To determine potential roles of GalNAc-T3 in Cd-induced FGF23 production, we examined the effect of Cd on GalNAc-T3 mRNA expression in vivo and in vitro. GalNAc-T3 gene expression was significantly increased in the bones of Cd-injected mice. Cd also enhanced the expression of GalNAc-T3 in cultured osteosarcoma UMR106 cells and primary osteocytes. Cd activated aryl hydrocarbon receptors (AhR) and AhR were required for GalNAc-T3 gene expression induced by Cd. In addition, Cd-dependent FGF23 production was completely inhibited by an AhR antagonist. AhR siRNA markedly suppressed the stimulation of transcriptional activity by Cd. Furthermore, Cd induced AhR activation via phosphorylation of Ser-68 by p38 kinase in the nuclear export signal of AhR. Thus, Cd stimulated GalNAc-T3 gene transcription via enhanced AhR binding to the GalNAc-T3 promoter. These findings suggest that the Cd-induced increase in GalNAc-T3 suppresses proteolytic processing of FGF23 and increases serum FGF23 concentrations.
(キーワード): Animals / Cadmium Chloride / Cell Culture Techniques / Cell Line, Tumor / Female / Femur / Fibroblast Growth Factors / Gene Expression / Mice, Inbred C57BL / Mice, Inbred ICR / N-Acetylgalactosaminyltransferases / Osteoblasts / Osteocytes / Phosphorylation / Receptors, Aryl Hydrocarbon / Up-Regulation / p38 Mitogen-Activated Protein Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/toxsci/kfu043
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24614234; ● Summary page in Scopus @ Elsevier: 2-s2.0-84899983795

(DOI: 10.1093/toxsci/kfu043, PubMed: 24614234, Elsevier: Scopus) Tomoyuki Kawamura, Moemi Hayashi, Rie Mukai, Junji Terao and Hisao Nemoto :
The First Synthesis of Uralenol, 5'-Prenylated Quercetin via Palladium-catalyzed O-dimethylallylation Reaction with Concurrent Acetyl Migration,
Synthesis, Vol.46, No.02, 170-174, 2014.

(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-0033-1338559
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84893833743

(DOI: 10.1055/s-0033-1338559, Elsevier: Scopus) Akari Ishisaka, Kyuichi Kawabata, Satomi Miki, Yuko Shiba, Shoko Minekawa, Tomomi Nishikawa, Rie Mukai, Junji Terao and Yoshichika Kawai :
Mitochondrial dysfunction leads to deconjugation of quercetin glucuronides in inflammatory macrophages.,
PLoS ONE, Vol.8, No.11, e80843, 2013.

(要約): Dietary flavonoids, such as quercetin, have long been recognized to protect blood vessels from atherogenic inflammation by yet unknown mechanisms. We have previously discovered the specific localization of quercetin-3-O-glucuronide (Q3GA), a phase II metabolite of quercetin, in macrophage cells in the human atherosclerotic lesions, but the biological significance is poorly understood. We have now demonstrated the molecular basis of the interaction between quercetin glucuronides and macrophages, leading to deconjugation of the glucuronides into the active aglycone. In vitro experiments showed that Q3GA was bound to the cell surface proteins of macrophages through anion binding and was readily deconjugated into the aglycone. It is of interest that the macrophage-mediated deconjugation of Q3GA was significantly enhanced upon inflammatory activation by lipopolysaccharide (LPS). Zymography and immunoblotting analysis revealed that -glucuronidase is the major enzyme responsible for the deglucuronidation, whereas the secretion rate was not affected after LPS treatment. We found that extracellular acidification, which is required for the activity of -glucuronidase, was significantly induced upon LPS treatment and was due to the increased lactate secretion associated with mitochondrial dysfunction. In addition, the -glucuronidase secretion, which is triggered by intracellular calcium ions, was also induced by mitochondria dysfunction characterized using antimycin-A (a mitochondrial inhibitor) and siRNA-knockdown of Atg7 (an essential gene for autophagy). The deconjugated aglycone, quercetin, acts as an anti-inflammatory agent in the stimulated macrophages by inhibiting the c-Jun N-terminal kinase activation, whereas Q3GA acts only in the presence of extracellular -glucuronidase activity. Finally, we demonstrated the deconjugation of quercetin glucuronides including the sulfoglucuronides in vivo in the spleen of mice challenged with LPS. These results showed that mitochondrial dysfunction plays a crucial role in the deconjugation of quercetin glucuronides in macrophages. Collectively, this study contributes to clarifying the mechanism responsible for the anti-inflammatory activity of dietary flavonoids within the inflammation sites.
(キーワード): Animals / Anti-Inflammatory Agents / Biological Transport / Calcium / Cell Line / Glucuronidase / Humans / Inflammation / Macrophages / Mice / Mitochondria / Quercetin
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0080843
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24260490; ● Search Scopus @ Elsevier (PMID): 24260490; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0080843

(DOI: 10.1371/journal.pone.0080843, PubMed: 24260490) Rie Mukai, Yutaka Fujikura, Kaeko Murota, Shoko Minekawa, Naoko Matsui, Hisao Nemoto and Junji Terao :
Prenylation enhances quercetin uptake and reduces efflux in Caco-2 cells and enhances tissue accumulation in mice fed long-term.,
The Journal of Nutrition, Vol.143, No.10, 1558-1564, 2013.

(要約): Prenyl flavonoids are widely distributed in plant foods and have attracted appreciable attention in relation to their potential benefits for human health. Prenylation may enhance the biological functions of flavonoids by introducing hydrophobic properties in their basic structures. Previously, we found that 8-prenyl naringenin exerted a greater preventive effect on muscle atrophy than nonprenylated naringenin in a mouse model. Here, we aimed to estimate the effect of prenylation on the bioavailability of dietary quercetin (Q). The cellular uptake of 8-prenyl quercetin (PQ) and Q in Caco-2 cells and C2C12 myotube cells was examined. Prenylation significantly enhanced the cellular uptake by increasing the lipophilicity in both cell types. In Caco-2 cells, efflux of PQ to the basolateral side was <15% of that of Q, suggesting that prenylation attenuates transport from the intestine to the circulation. After intragastric administration of PQ or Q to mice or rats, the area under the concentration-time curve for PQ in plasma and lymph was 52.5% and 37.5% lower than that of Q, respectively. PQ and its O-methylated form (MePQ) accumulated at much higher amounts than Q and O-methylated Q in the liver (Q: 3400%; MePQ: 7570%) and kidney (Q: 385%; MePQ: 736%) of mice after 18 d of feeding. These data suggest that prenylation enhances the accumulation of Q in tissues during long-term feeding, even though prenylation per se lowers its intestinal absorption from the diet.
(キーワード): Animals / Area Under Curve / Biological Availability / Biological Transport / Caco-2 Cells / Humans / Hydrophobic and Hydrophilic Interactions / Intestinal Absorption / Intestinal Mucosa / Kidney / Liver / Lymph / Methylation / Mice / Mice, Inbred C57BL / Muscle Fibers, Skeletal / Prenylation / Quercetin / Rats / Rats, Wistar
(徳島大学機関リポジトリ): ● Metadata: 112457
(出版サイトへのリンク): ● Publication site (DOI): 10.3945/jn.113.176818
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23902958; ● Search Scopus @ Elsevier (PMID): 23902958; ● Search Scopus @ Elsevier (DOI): 10.3945/jn.113.176818

(徳島大学機関リポジトリ: 112457, DOI: 10.3945/jn.113.176818, PubMed: 23902958) Tomoki Abe, Shohei Kohno, Tomonari Yama, Arisa Ochi, Takuro Suto, Katsuya Hirasaka, Ayako Ohno, Shigetada Teshima-Kondo, Yuushi Okumura, Motoko Oarada, Inho Choi, Rie Mukai, Junji Terao and Takeshi Nikawa :
Soy Glycinin Contains a Functional Inhibitory Sequence against Muscle-Atrophy-Associated Ubiquitin Ligase Cbl-b.,
International Journal of Endocrinology, Vol.2013, 907565, 2013.

(要約): Background. Unloading stress induces skeletal muscle atrophy. We have reported that Cbl-b ubiquitin ligase is a master regulator of unloading-associated muscle atrophy. The present study was designed to elucidate whether dietary soy glycinin protein prevents denervation-mediated muscle atrophy, based on the presence of inhibitory peptides against Cbl-b ubiquitin ligase in soy glycinin protein. Methods. Mice were fed either 20% casein diet, 20% soy protein isolate diet, 10% glycinin diet containing 10% casein, or 20% glycinin diet. One week later, the right sciatic nerve was cut. The wet weight, cross sectional area (CSA), IGF-1 signaling, and atrogene expression in hindlimb muscles were examined at 1, 3, 3.5, or 4 days after denervation. Results. 20% soy glycinin diet significantly prevented denervation-induced decreases in muscle wet weight and myofiber CSA. Furthermore, dietary soy protein inhibited denervation-induced ubiquitination and degradation of IRS-1 in tibialis anterior muscle. Dietary soy glycinin partially suppressed the denervation-mediated expression of atrogenes, such as MAFbx/atrogin-1 and MuRF-1, through the protection of IGF-1 signaling estimated by phosphorylation of Akt-1. Conclusions. Soy glycinin contains a functional inhibitory sequence against muscle-atrophy-associated ubiquitin ligase Cbl-b. Dietary soy glycinin protein significantly prevented muscle atrophy after denervation in mice.
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2013/907565
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23762056; ● Search Scopus @ Elsevier (PMID): 23762056; ● Search Scopus @ Elsevier (DOI): 10.1155/2013/907565

(DOI: 10.1155/2013/907565, PubMed: 23762056) Katsuya Hirasaka, Tasuku Maeda, Chika Ikeda, Marie Haruna, Arisa Ochi, Rie Mukai, Motoko Oarada, Shigetada Kondo, Ayako Ohno, Yuushi Okumura, Junji Terao and Takeshi Nikawa :
Isoflavones derived from soy beans prevent MuRF1-mediated muscle atrophy in C2C12 myotubes through SIRT1 activation.,
Journal of Nutritional Science and Vitaminology, Vol.59, No.4, 317-324, 2013.

(要約): Proinflammatory cytokines are factors that induce ubiquitin-proteasome-dependent proteolysis in skeletal muscle, causing muscle atrophy. Although isoflavones, as potent antioxidative nutrients, have been known to reduce muscle damage during the catabolic state, the non-antioxidant effects of isoflavones against muscle atrophy are not well known. Here we report on the inhibitory effects of isoflavones such as genistein and daidzein on muscle atrophy caused by tumor necrosis factor (TNF)- treatment. In C2C12 myotubes, TNF- treatment markedly elevated the expression of the muscle-specific ubiquitin ligase MuRF1, but not of atrogin-1, leading to myotube atrophy. We found that MuRF1 promoter activity was mediated by acetylation of p65, a subunit of NFB, a downstream target of the TNF- signaling pathway; increased MuRF1 promoter activity was abolished by SIRT1, which is associated with deacetylation of p65. Of interest, isoflavones induced expression of SIRT1 mRNA and phosphorylation of AMP kinase, which is well known to stimulate SIRT1 expression, although there was no direct effect on SIRT1 activation. Moreover, isoflavones significantly suppressed MuRF1 promoter activity and myotube atrophy induced by TNF- in C2C12 myotubes. These results suggest that isoflavones suppress myotube atrophy in skeletal muscle cells through activation of SIRT1 signaling. Thus, the efficacy of isoflavones could provide a novel therapeutic approach against inflammation-related muscle atrophy.
(キーワード): Acetylation / Adenylate Kinase / Animals / Antioxidants / Cell Line / Inflammation / Isoflavones / Mice / Muscle Fibers, Skeletal / Muscle Proteins / Muscular Atrophy / NF-kappa B / Phosphorylation / Phytotherapy / Plant Extracts / Promoter Regions, Genetic / RNA, Messenger / SKP Cullin F-Box Protein Ligases / Signal Transduction / Sirtuin 1 / Soybeans / Transcription Factor RelA / Tumor Necrosis Factor-alpha / Ubiquitin-Protein Ligases
(出版サイトへのリンク): ● Publication site (DOI): 10.3177/jnsv.59.317
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24064732; ● CiNii @ 国立情報学研究所 (CRID): 1390001206324728576; ● Summary page in Scopus @ Elsevier: 2-s2.0-84884576219

(DOI: 10.3177/jnsv.59.317, PubMed: 24064732, CiNii: 1390001206324728576, Elsevier: Scopus) Yauhen Bandaruk, Rie Mukai, Tomoyuki Kawamura, Hisao Nemoto and Junji Terao :
Evaluation of the inhibitory effects of quercetin-related flavonoids and tea catechins on the monoamine oxidase-A reaction in mouse brain mitochondria.,
Journal of Agricultural and Food Chemistry, Vol.60, No.41, 10270-10277, 2012.

(要約): Quercetin, a typical dietary flavonoid, is thought to exert antidepressant effects by inhibiting the monoamine oxidase-A (MAO-A) reaction, which is responsible for regulation of the metabolism of the neurotransmitter 5-hydroxytryptamine (5-HT) in the brain. This study compared the MAO-A inhibitory activity of quercetin with those of O-methylated quercetin (isorhamnetin, tamarixetin), luteolin, and green tea catechins ((-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, and (-)-epigallocatechin gallate) by measuring the formation of the oxidative deamination product of 5-HT, 5-hydroxyindole aldehyde (5-HIAL), in mouse brain mitochondria. Quercetin was inferior to luteolin in the inhibition of MAO-A activity, whereas isorhamnetin, tamarixetin, and tea catechins scarcely exerted inhibitory activity. Quercetin did not affect MAO-A activity in mouse intestinal mitochondria, indicating that it does not evoke side effects on the metabolism of dietary monoamines in the gut. These data suggest that quercetin is a weak (but safe) MAO-A inhibitor in the modulation of 5-HT levels in the brain.
(キーワード): Animals / 脳 (brain) / カテキン (catechin) / フラボノイド (flavonoids) / Humans / Intestine, Small / Male / Mice / Mice, Inbred C57BL / ミトコンドリア (mitochondria) / Monoamine Oxidase / Monoamine Oxidase Inhibitors / Quercetin / Recombinant Proteins / Tea
(徳島大学機関リポジトリ): ● Metadata: 106304
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf303055b
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23009399; ● Search Scopus @ Elsevier (PMID): 23009399; ● Search Scopus @ Elsevier (DOI): 10.1021/jf303055b

(徳島大学機関リポジトリ: 106304, DOI: 10.1021/jf303055b, PubMed: 23009399) Rie Mukai, Hitomi Horikawa, Yutaka Fujikura, Tomoyuki Kawamura, Hisao Nemoto, Takeshi Nikawa and Junji Terao :
Prevention of disuse muscle atrophy by dietary ingestion of 8-prenylnaringenin in denervated mice,
PLoS ONE, Vol.7, No.9, e45048, 2012.

(要約): Flavonoids have attracted considerable attention in relation to their effects upon health. 8-Prenylnaringenin (8-PN) is found in the common hop (Humulus lupulus) and assumed to be responsible for the health impact of beer consumption. We wanted to clarify the effects of prenylation on the physiological functions of dietary flavonoids by comparing the effects of 8-PN with that of intact naringenin in the prevention of disuse muscle atrophy using a model of denervation in mice. Consumption of 8-PN (but not naringenin) prevented loss of weight in the gastrocnemius muscle further supported by the lack of induction of the protein content of a key ubiquitin ligase involved in muscle atrophy, atrogin-1, and by the activation of Akt phosphorylation. 8-PN content in the gastrocnemius muscle was tenfold higher than that of naringenin. These results suggested that, compared with naringenin, 8-PN was effectively concentrated into skeletal muscle to exert its preventive effects upon disuse muscle atrophy. It is likely that prenylation generates novel functions for 8-PN by enhancing its accumulation into muscle tissue through dietary intake.
(キーワード): Animals / Cell Line / Chromatography, High Pressure Liquid / Diet / Flavanones / Humulus / Male / Mice / Mice, Inbred C57BL / Muscle Denervation / Muscle Fibers, Skeletal / Muscle, Skeletal / Muscular Atrophy / Muscular Disorders, Atrophic / Phosphorylation / Powders / Proto-Oncogene Proteins c-akt / Water
(徳島大学機関リポジトリ): ● Metadata: 110158
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0045048
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23028754; ● Search Scopus @ Elsevier (PMID): 23028754; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0045048

(徳島大学機関リポジトリ: 110158, DOI: 10.1371/journal.pone.0045048, PubMed: 23028754) Shin Nishiumi, Rie Mukai, Takashi Ichiyanagi and Hitoshi Ashida :
Suppression of lipopolysaccharide and galactosamine-induced hepatic inflammation by red grape pomace.,
Journal of Agricultural and Food Chemistry, Vol.60, No.36, 9315-9320, 2012.

(要約): Grape pomace is generated in the production process of wine and grape juices and is an industrial waste. This study investigated whether an intake of grape pomace was able to suppress chronic inflammation induced by lipopolysaccharide (LPS) and galactosamine (GalN) in vivo. When Sprague-Dawley rats were orally given methanolic extracts from red and white grape pomace, the extracts inhibited the LPS/GalN-evoked activation of nuclear factor-κB (NF-κB) dose-dependently, and red grape pomace exerted a stronger effect than white grape one. Next, rats were fed an AIN93 M-based diet containing 5% red grape pomace for 7 days, followed by the intraperitoneal injection of LPS and GalN. The intake of the red grape pomace-supplemented diet was found to suppress the LPS/GalN-induced activation of NF-κB and expression of inducible nitric oxide synthase and cyclooxygenase-2 proteins. These results suggest that red grape pomace may contain an abundance of effective compound(s) for anti-inflammatory action.
(キーワード): Animals / Anti-Inflammatory Agents / Cyclooxygenase 2 / Down-Regulation / Galactosamine / Humans / Industrial Waste / Lipopolysaccharides / Liver / Liver Diseases / Male / Nitric Oxide Synthase / Plant Extracts / Rats / Rats, Sprague-Dawley / Vitis
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf302298n
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22928618; ● Search Scopus @ Elsevier (PMID): 22928618; ● Search Scopus @ Elsevier (DOI): 10.1021/jf302298n

(DOI: 10.1021/jf302298n, PubMed: 22928618) Tomoyuki Kawamura, Moemi Hayashi, Rie Mukai, Junji Terao and Hisao Nemoto :
An Efficient Method for C8-Prenylation of Flavonols and Flavanones,
Synthesis, Vol.44, No.9, 1308-1314, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-0031-1290756
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1055/s-0031-1290756

(DOI: 10.1055/s-0031-1290756) Rie Mukai, Kyuichi Kawabata, Seiko Otsuka, Akari Ishisaka, Yoshichika Kawai, Zai-Si Ji, Hiroshi Tsuboi and Junji Terao :
Effect of quercetin and its glucuronide metabolite upon 6-hydorxydopamine-induced oxidative damage in Neuro-2a cells.,
Free Radical Research, Vol.46, No.8, 1019-1028, 2012.

(要約): Quercetin is ubiquitously distributed in plant foods. This antioxidative polyphenol is mostly converted to conjugated metabolites in the body. Parkinson disease (PD) has been suggested to be related to oxidative stress derived from abnormal dopaminergic activity. We evaluated if dietary quercetin contributes to the antioxidant network in the central nervous system from the viewpoint of PD prevention. A neurotoxin, 6-hydroxydopamine (6-OHDA), was used as a model of PD. 6-OHDA-induced H(2)O(2) production and cell death in mouse neuroblastoma, Neuro-2a. Quercetin aglycone suppressed 6-OHDA-induced H(2)O(2) production and cell death, although aglycone itself reduced cell viability at higher concentration. Quercetin 3-O-β-d-glucuronide (Q3GA), which is an antioxidative metabolite of dietary quercetin, was little incorporated into the cell resulting in neither suppression of 6-OHDA-induced cell death nor reduction of cell viability. Q3GA was found to be deconjugated to quercetin by microglial MG-6 cells. These results indicate that quercetin metabolites should be converted to their aglycone to exert preventive effect on damage to neuronal cells.
(出版サイトへのリンク): ● Publication site (DOI): 10.3109/10715762.2012.673720
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22404304; ● Search Scopus @ Elsevier (PMID): 22404304; ● Search Scopus @ Elsevier (DOI): 10.3109/10715762.2012.673720

(DOI: 10.3109/10715762.2012.673720, PubMed: 22404304) Shin Nishiumi, Keizo Hosokawa, Masaki Anetai, Toshiro Shibata, Rie Mukai, Ken-ichi Yoshida and Hitoshi Ashida :
Antagonistic effect of the Ainu-selected traditional beneficial plants on the transformation of an aryl hydrocarbon receptor.,
Journal of Food Science, Vol.77, No.4, C420-429, 2012.

(要約): Transformation of an aryl hydrocarbon receptor (AhR) is the initial step to express the multiple toxicity of halogenated and polycyclic aromatic hydrocarbons (HAHs and PAHs) including dioxins. Therefore, it has been suggested that suppression of the transformation induced by HAHs and PAHs leads to reduce their toxicological effects. In this study, the antagonistic effect of 110 indigenous plants (192 plant parts) used as medicine and/or food by the Ainu on the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR transformation was investigated. Of these, a stalk of Aralia elata (Miq.) Seemann and a bark of Fraxinus mandshurica Rupr. var. japonica Maxim. exhibited the strong antagonistic effect in a dose-dependent manner. An antioxidative activity and polyphenol content were also measured, and the strong correlation (r= 0.96) between these two parameters could be confirmed. However, correlation coefficients of the antagonistic effect of 192 extracts compared to their antioxidative activity and polyphenol content were 0.17 and 0.20, respectively. These results suggest that the Ainu-selected traditional beneficial plants are useful source for findings of novel AhR antagonists, and the antagonistic activity of these plants may be independent on their antioxidative activity and polyphenol content. PRACTICAL APPLICATION: Our findings lead to discovery of the valuable plants used by the Ainu and the novel active compounds useful for human's life, and furthermore, may contribute to the development of new medicines and functional foods.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1750-3841.2011.02611.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22394227; ● Search Scopus @ Elsevier (PMID): 22394227; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1750-3841.2011.02611.x

(DOI: 10.1111/j.1750-3841.2011.02611.x, PubMed: 22394227) Hiroko Takumi, Hiroyasu Nakamura, Terumi Simizu, Ryoko Harada, Takashi Kometani, Tomonori Nadamoto, Rie Mukai, Kaeko Murota, Yoshichika Kawai and Junji Terao :
Bioavailability of orally administered water-dispersible hesperetin and its effect on peripheral vasodilatation in human subjects: implication of endothelial functions of plasma conjugated metabolites.,
Food & Function, Vol.3, No.4, 389-398, 2012.

(要約): Hesperetin is an aglycone of citrus flavonoids and is expected to exert a vasodilatation effect in vivo. We developed water-dispersible hesperetin by the process of micronization to enhance the bioavailability of hesperetin. This study aimed to assess the effect of this process on the bioavailability of hesperetin and to estimate its efficiency on vasodilatation-related functions using endothelial cells in vitro and a human volunteer study at a single dose in vivo. We found that water-dispersible hesperetin was absorbed rapidly, with its maximum plasma concentration (C(max)) being 10.2 ± 1.2 μM, and that the time to reach C(max), which is within 1 h if 150 mg of this preparation was orally administered in humans. LC-MS analyses of the plasma at C(max) demonstrated that hesperetin accumulated in the plasma as hesperetin 7-O-β-D-glucuronide (Hp7GA), hesperetin 3'-O-β-D-glucuronide (Hp3'GA) and hesperetin sulfate exclusively. Similar to hesperetin, Hp7GA enhanced nitric oxide (NO) release by inhibiting nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) activity in a human umbilical vein endothelial cell culture system, indicating that plasma hesperetin metabolites can improve vasodilatation in the vascular system. A volunteer study using women with cold sensitivity showed that a single dose of water-dispersible hesperetin was effective on peripheral vasodilatation.These results strongly suggest that rapid accumulation with higher plasma concentration enables hesperetin to exert a potential vasodilatation effect by the endothelial action of its plasma metabolites. Water-dispersible hesperetin may be useful to improve the health effect of dietary hesperetin.
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/c2fo10224b
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22307524; ● Search Scopus @ Elsevier (PMID): 22307524; ● Search Scopus @ Elsevier (DOI): 10.1039/c2fo10224b

(DOI: 10.1039/c2fo10224b, PubMed: 22307524) Hiroko Takumi, Rie Mukai, Sawako Ishiduka, Takashi Kometani and Junji Terao :
Tissue distribution of hesperetin in rats after a dietary intake.,
Bioscience, Biotechnology, and Biochemistry, Vol.75, No.8, 1608-1610, 2011.

(要約): Hesperetin, the aglycone of hesperidin present in citrus fruits, possesses various biological activities. We assessed the tissue distribution of hesperetin in rats fed with a 0.2% hesperetin diet for 4 weeks. Its highest concentration was found in the liver, and the second highest was in the aorta. The aorta is assumed to be one of the main target tissues of hesperetin for exerting its functions.
(キーワード): Animals / Aorta / Biological Availability / Capillary Permeability / Cardiovascular Diseases / Chromatography, High Pressure Liquid / Citrus / Diet / Hesperidin / Liver / Male / Rats / Rats, Wistar / Tissue Distribution
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.110157
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21821945; ● Search Scopus @ Elsevier (PMID): 21821945; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.110157

(DOI: 10.1271/bbb.110157, PubMed: 21821945) Shohei Kohno, Tatsuya Ueji, Tomoki Abe, Reiko Nakao, Katsuya Hirasaka, Motoko Oarada, Akiko Harada, Ayako Maita, Akira Higashibata, Rie Mukai, Junji Terao, Yuushi Okumura and Takeshi Nikawa :
Rantes secreted from macrophages disturbs skeletal muscle regeneration after cardiotoxin injection in Cbl-b-deficient mice,
Muscle & Nerve, Vol.43, No.2, 223-229, 2011.

(要約): Deficiency of the Cbl-b ubiquitin ligase gene activates macrophages in mice. This study aimed to elucidate the pathophysiological roles of macrophages in muscle degeneration/regeneration in Cbl-b-deficient mice. We examined immune cell infiltration and cytokine expression in cardiotoxin-injected tibialis anterior muscle of Cbl-b-deficient mice. Ablation of the Cbl-b gene expression delayed regeneration of cardiotoxin-induced skeletal muscle damage compared with wild-type mice. CD8-positive T cells were still present in the damaged muscle on day 14 after cardiotoxin injection in Cbl-b-deficient mice, but there was dispersal of the same cells over that time-frame in wild-type mice. Infiltrating macrophages in Cbl-b-deficient mice showed strong expression of RANTES (regulated-on-activation, normal T cell expressed and secreted), a chemokine for CD8-positive T cells. In turn, a neutralizing antibody against RANTES significantly suppressed the infiltration of CD8-positive T cells into the muscle, resulting in restoration of the disturbed muscle regeneration. Cbl-b is an important regulatory factor for cytotoxic T-cell infiltration via RANTES production in macrophages.
(キーワード): Adaptor Proteins, Signal Transducing / 分散分析 (analysis of variance) / Animals / Antibodies / Antigens, CD / Cardiotoxins / Chemokine CCL5 / 細胞質分裂 (cytokinesis) / Disease Models, Animal / Dose-Response Relationship, Drug / Gene Expression Regulation / Macrophages / Mice / Mice, Inbred C57BL / ノックアウトマウス (knockout mice) / Muscle, Skeletal / Muscular Diseases / Myogenic Regulatory Factors / Proto-Oncogene Proteins c-cbl / RNA, Messenger / 再生 (regeneration) / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/mus.21829
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21254087; ● Summary page in Scopus @ Elsevier: 2-s2.0-78751674465

(DOI: 10.1002/mus.21829, PubMed: 21254087, Elsevier: Scopus) Rie Mukai, Reiko Nakao, Hironori Yamamoto, Takeshi Nikawa, Eiji Takeda and Junji Terao :
Quercetin Prevents Unloading-Derived Disused Muscle Atrophy by Attenuating the Induction of Ubiquitin Ligases in Tail-Suspension Mice.,
Journal of Natural Products, Vol.73, No.10, 1708-1710, 2010.

(要約): The effects of quercetin (1) were investigated on disused muscle atrophy using mice that underwent tail suspension. Periodic injection of 1 into the gastrocnemius muscle suppressed muscle weight loss and ubiquitin ligase expression. Compound 1 reduced the enhancement of lipid peroxidation in the muscle. Injection of N-acetyl-l-cysteine, but not flavone (2), also prevented muscle weight loss and enhancement of lipid peroxidation. These findings demonstrate that 1 can prevent disused muscle atrophy by attenuating the expression of ubiquitin ligases and that such prevention originates from its antioxidant activity.
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/np100240y
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20853873; ● Search Scopus @ Elsevier (PMID): 20853873; ● Search Scopus @ Elsevier (DOI): 10.1021/np100240y

(DOI: 10.1021/np100240y, PubMed: 20853873) Nhung Thuy Dang, Rie Mukai, Ken-Ichi Yoshida and Hitoshi Ashida :
D-pinitol and myo-inositol stimulate translocation of glucose transporter 4 in skeletal muscle of C57BL/6 mice.,
Bioscience, Biotechnology, and Biochemistry, Vol.74, No.5, 1062-1067, 2010.

(要約): Diabetes mellitus is a complex disease that is characterized by the defection of insulin sensitivity in such peripheral tissues as skeletal muscle, adipose tissue and liver. We have previously demonstrated that certain inositol derivatives stimulated glucose uptake accompanied by the translocation of glucose transporter 4 (GLUT4) to the plasma membrane in L6 myotubes. We investigated in this present study whether an oral intake of D-pinitol (PI) and myo-inositol (MI) would affect GLUT4 translocation in the skeletal muscle of mice. PI or MI at 1 g/kg BW administered orally to mice 30 min before a post-oral injection of glucose at 2 g/kg BW resulted in both PI and MI increasing GLUT4 translocation in the skeletal muscle and lowering the plasma glucose and insulin levels. PI and MI, therefore, have the potential to prevent diabetes mellitus by reducing the postprandial blood glucose level and stimulating GLUT4 translocation in the skeletal muscle.
(キーワード): Administration, Oral / Animals / Blood Glucose / Glucose Transporter Type 4 / Inositol / インスリン (insulin) / Male / Mice / Mice, Inbred C57BL / Muscle, Skeletal / Protein Transport / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.90963
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20460718; ● CiNii @ 国立情報学研究所 (CRID): 1390282681454674176; ● Search Scopus @ Elsevier (PMID): 20460718; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.90963

(DOI: 10.1271/bbb.90963, PubMed: 20460718, CiNii: 1390282681454674176) Rie Mukai, Yasuhito Shirai, Naoaki Saito, Itsuko Fukuda, Shin Nishiumi, Ken-Ichi Yoshida and Hitoshi Ashida :
Suppression mechanisms of flavonoids on aryl hydrocarbon receptor-mediated signal transduction.,
Archives of Biochemistry and Biophysics, Vol.501, No.1, 134-141, 2010.

(要約): The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates biological and toxicological effects by binding to its agonists such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Previously we demonstrated that flavonoids suppressed the TCDD-induced DNA-binding activity of the AhR in a structure-dependent manner. In this study, we investigated the mechanisms by which flavonoids suppressed the AhR-mediated signal transduction in mouse hepatoma Hepa-1c1c7 cells. Flavones and flavonols suppressed the TCDD-induced nuclear translocation of the AhR and dissociation of its partner proteins, heat shock protein 90 and X-associated protein 2, whereas flavanones and catechins did not. Flavonoids of all these four subclasses suppressed the phosphorylation of both AhR and Arnt and the formation of a heterodimer consisting of these proteins. Since certain flavonoids are known to inhibit mitogen-activated protein kinases (MAPKs), we confirmed the contribution of MAPK/ERK kinase (MEK) to the AhR-mediated signal transduction by using U0126, an inhibitor of MEK1/2. U0126 suppressed TCDD-induced phosphorylation of the AhR and Arnt followed by the DNA-binding activity of the AhR. Flavanones and catechins suppressed the TCDD-induced phosphorylation of ERK1/2. The inhibition of MEK/ERK phosphorylation is one of the mechanisms by which flavanones and catechins suppress the AhR-mediated signal transduction in Hepa-1c1c7 cells.
(キーワード): Active Transport, Cell Nucleus / Animals / Aryl Hydrocarbon Receptor Nuclear Translocator / Base Sequence / カテキン (catechin) / Cell Line, Tumor / DNA Primers / Flavanones / フラボノイド (flavonoids) / MAP Kinase Signaling System / Mice / リン酸化 (phosphorylation) / Receptors, Aryl Hydrocarbon / Recombinant Proteins / シグナル伝達 (signal transduction) / Tetrachlorodibenzodioxin / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.abb.2010.05.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20450880; ● Search Scopus @ Elsevier (PMID): 20450880; ● Search Scopus @ Elsevier (DOI): 10.1016/j.abb.2010.05.002

(DOI: 10.1016/j.abb.2010.05.002, PubMed: 20450880) Rie Mukai, Hideo Satsu, Makoto Shimizu and Hitoshi Ashida :
Inhibition of P-glycoprotein enhances the suppressive effect of kaempferol on transformation of the aryl hydrocarbon receptor.,
Bioscience, Biotechnology, and Biochemistry, Vol.73, No.7, 1635-1639, 2009.

(要約): Dioxins enter the body mainly through the diet, bind to the aryl hydrocarbon receptor (AhR), and cause various toxicological effects. In this study, we found that oral administration of kaempferol or ginkgo biloba extract (EGb) containing 24% flavonol at 100 mg/kg body weight suppressed AhR transformation induced by 3-methylcholanthrene at 10 mg/kg body weight in the liver of mice. The suppressive effect of kaempferol was enhanced by verapamil, an inhibitor of P-glycoprotein (P-gp), in ex vivo experiments using a hepatic cytosolic fraction and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Enhancement of the suppressive effect by verapamil was also observed in mouse hepatoma Hepa-1c1c7 cells, accompanied by an increase in the uptake of kaempferol into the cells. In conclusion, inhibition of P-gp enhanced the suppressive effect of kaempferol on AhR transformation through an increase in the intracellular kaempferol concentration.
(キーワード): Animals / Biological Transport / Cell Line, Tumor / Ginkgo biloba / Kaempferols / Liver / Male / Methylcholanthrene / Mice / Mice, Inbred C57BL / P-Glycoprotein / Plant Extracts / Receptors, Aryl Hydrocarbon / Verapamil
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.90145
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19584540; ● CiNii @ 国立情報学研究所 (CRID): 1390001206479140352; ● Search Scopus @ Elsevier (PMID): 19584540; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.90145

(DOI: 10.1271/bbb.90145, PubMed: 19584540, CiNii: 1390001206479140352) Rie Mukai, Yasuhito Shirai, Naoaki Saito, Ken-Ichi Yoshida and Hitoshi Ashida :
Subcellular localization of flavonol aglycone in hepatocytes visualized by confocal laser scanning fluorescence microscope.,
Cytotechnology, Vol.59, No.3, 177-182, 2009.

(要約): Flavonoids are widely distributed in the plant kingdom and show various biological activities. The bioavailability of flavonoids in biological samples has conventionally been quantified by high-performance liquid chromatography and mass spectrometry, but with these analytical techniques it is difficult to estimate the subcellular localization of flavonoids in intact cells. In this study, we attempted to examine the localization of flavonoids in cultured cells using a confocal laser scanning fluorescence microscope and mouse hepatoma Hepa-1c1c7 cells. Five flavonol aglycones showed autofluorescence in the cells under the conditions (Ex. 488 nm to Em. 515-535 nm), whereas three flavonol glycosides and eight compounds belonging to other flavonoid subclasses, i.e., flavones, flavanones, and catechins, did not. The autofluorescence of galangin and kaempferol appeared stronger in the nucleus than cytoplasm, suggesting that they are incorporated into the cells and accumulated in the nucleus. The proposed method provided evidence that flavonol aglycones are incorporated into, and accumulated in the nucleus of, hepatocytes.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10616-009-9206-z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19568944; ● Search Scopus @ Elsevier (PMID): 19568944; ● Search Scopus @ Elsevier (DOI): 10.1007/s10616-009-9206-z

(DOI: 10.1007/s10616-009-9206-z, PubMed: 19568944) Rie Mukai, Itsuko Fukuda, Shin Nishiumi, Midori Natsume, Naomi Osakabe, Ken-ichi Yoshida and Hitoshi Ashida :
Cacao polyphenol extract suppresses transformation of an aryl hydrocarbon receptor in C57BL/6 mice.,
Journal of Agricultural and Food Chemistry, Vol.56, No.21, 10399-10405, 2008.

(要約): Dioxins enter the body through the diet and cause various toxicological effects through transformation of an aryl hydrocarbon receptor (AhR). Plant extracts and phytochemicals including flavonoids are reported to suppress this transformation. This paper investigates the suppression by a cacao polyphenol extract (CPE) of AhR transformation in vivo. The CPE was administered orally to C57BL/6 mice at 100 mg/kg of body weight, followed 1 h later by 3-methylcholanthrene (MC), an AhR agonist, injected intraperitoneally at 10 mg/kg of body weight. CPE suppressed the MC-induced transformation to the control level by inhibiting the formation of a heterodimer between AhR and an aryl hydrocarbon receptor nuclear translocator in the liver at 3 h postadministration. It also suppressed MC-induced cytochrome P4501A1 expression and NAD(P)H:quinone-oxidoreductase activity, whereas it increased glutathione S-transferase activity at 25 h. CPE constituents and their metabolites might contribute, at least in part, to the suppression of AhR transformation. The results indicate that the intake of CPE suppressed the toxicological effects of dioxins in the body.
(キーワード): Animals / Cacao / Cell Line, Tumor / Cell Transformation, Neoplastic / Dimerization / ダイオキシン類 (dioxins) / Down-Regulation / フラボノイド (flavonoids) / 遺伝子発現 (gene expression) / Humans / Liver / Male / Mice / Mice, Inbred C57BL / Phenols / Plant Extracts / Polyphenols / Protein Binding / Protein Transport / Random Allocation / Receptors, Aryl Hydrocarbon
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf802453t
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18928297; ● Search Scopus @ Elsevier (PMID): 18928297; ● Search Scopus @ Elsevier (DOI): 10.1021/jf802453t

(DOI: 10.1021/jf802453t, PubMed: 18928297) Itsuko Fukuda, Rie Mukai, Masaya Kawase, Ken-ichi Yoshida and Hitoshi Ashida :
Interaction between the aryl hydrocarbon receptor and its antagonists, flavonoids.,
Biochemical and Biophysical Research Communications, Vol.359, No.3, 822-827, 2007.

(要約): Flavonoids have been reported to be dietary antagonists of an aryl hydrocarbon receptor (AhR). However, little is known about the molecular mechanism on their antagonistic effects. In this study, the inhibitory effect of flavonoids on ligand binding to the AhR and interaction between flavonoids and the AhR complex (AhRc) were investigated in each flavonoid subclass. Flavone, flavonol, and flavanone but not catechin inhibited the specific binding between the AhR and 3-methylcholanthrene dose-dependently, indicating that the former three subclasses possibly act as competitive antagonists of the AhR. However, catechin in addition to the former three subclasses directly interacted with the AhRc by surface plasmon resonance analysis. The dissociation constant values showed an inverse correlation with the suppressive effect on the DNA binding activity. These results suggest that flavone, flavonol, and flavanone act as competitive antagonists of the AhR, while catechin associates with the AhRc and indirectly exhibits its antagonistic effects.
(キーワード): Animals / Cell Fractionation / Cytosol / フラボノイド (flavonoids) / Liver / Male / Methylcholanthrene / Protein Binding / Rats / Rats, Sprague-Dawley / Receptors, Aryl Hydrocarbon / Surface Plasmon Resonance
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2007.05.199
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17560542; ● Search Scopus @ Elsevier (PMID): 17560542; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2007.05.199

(DOI: 10.1016/j.bbrc.2007.05.199, PubMed: 17560542) Ken-Ichi Yoshida, Won-Seok Kim, Masaki Kinehara, Rie Mukai, Hitoshi Ashida, Hideki Ikeda, Yasutaro Fujita and Hari B. Krishnan :
Identification of a functional 2-keto-myo-inositol dehydratase gene of Sinorhizobium fredii USDA191 required for myo-inositol utilization.,
Bioscience, Biotechnology, and Biochemistry, Vol.70, No.12, 2957-2964, 2006.

(要約): Sinorhizobium fredii USDA191 is a Gram-negative bacterium capable of forming nitrogen-fixing nodules on soybean roots. The USDA191 idhA gene encoding myo-inositol dehydrogenase, an enzyme necessary for myo-inositol utilization, is known to be involved in competitive nodulation and nitrogen fixation. In Bacillus subtilis, myo-inositol dehydrogenase catalyzes the first step of the myo-inositol catabolic pathway. Recently iolE was identified as the gene encoding 2-keto-myo-inositol dehydratase, which catalyzes the second step in the pathway. Here we report the presence of 2-keto-myo-inositol dehydratase activity in free-living USDA191 cells cultured in a medium containing myo-inositol. An iolE ortholog was cloned from USDA191. USDA191 iolE was expressed in Escherichia coli as a His(6)-tag fusion and purified to exhibit 2-keto-myo-inositol dehydratase activity. Inactivation of USDA191 iolE led to defective myo-inositol utilization. USDA191 iolE partially complemented a B. subtilis iolE deficient mutant. These results suggest that S. fredii USDA191 utilizes a myo-inositol catabolic pathway, analogous to that of B. subtilis, involving at least idhA and iolE.
(キーワード): Amino Acid Sequence / Base Sequence / Cloning, Molecular / DNA Primers / Genes, Bacterial / Hydro-Lyases / Inositol / Molecular Sequence Data / Sequence Homology, Amino Acid / Sinorhizobium fredii
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.60362
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17151471; ● Search Scopus @ Elsevier (PMID): 17151471; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.60362

(DOI: 10.1271/bbb.60362, PubMed: 17151471) Shin Nishiumi, Keizo Hosokawa, Rie Mukai, Itsuko Fukuda, Atsuyuki Hishida, Osamu Iida, Ken-ichi Yoshida and Hitoshi Ashida :
Screening of indigenous plants from Japan for modulating effects on transformation of the aryl hydrocarbon receptor.,
Asian Pacific Journal of Cancer Prevention : APJCP, Vol.7, No.2, 208-220, 2006.

(要約): The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor with which halogenated and polycyclic aromatic hydrocarbons such as dioxins and benzo[a]pyrene interact as ligands. Since such compounds cause various toxicological effects, including cancer, through the transformation of AhR, it is important to determine influence of modulating factors. It has been reported that certain plant components such as flavonoids and indoles can affect AhR transformation. In this study, to obtain clues to novel ligands of AhR, 191 species of indigenous plants were collected in Japan, and their 50% methanolic extracts (total 368 plant parts) were tested for modulating effects on AhR transformation in a cell-free system using a rat hepatic cytosolic fraction. Among tested extracts at a concentration of 1 mg dry weight of plant/mL, 174 of 368 extracts suppressed 1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR transformation to 50% or less, while 9 extracts per se induced AhR transformation equivalent to more than 20% of that induced by 1 nM TCDD. Mallotus japonicus (Thunb.) Muell. (leaf) and Trichosanthes rostrata Kitamura (fruit and fruit skin) strongly suppressed 1 nM TCDD-induced AhR transformation, while Phellodendron amurense Ruprecht (seed) per se strongly induced AhR transformation. These results suggest that a large variety of plants in Japan contain various compounds modulating, mainly suppressing, AhR transformation.
(キーワード): Animals / Cell-Free System / Cytosol / Enzyme-Linked Immunosorbent Assay / 日本 (Japan) / Liver / Male / Plant Extracts / Rats / Rats, Sprague-Dawley / Receptors, Aryl Hydrocarbon
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16839212; ● Search Scopus @ Elsevier (PMID): 16839212

(PubMed: 16839212) Shin Nishiumi, Yoshiyuki Yabushita, Itsuko Fukuda, Rie Mukai, Ken-Ichi Yoshida and Hitoshi Ashida :
Molokhia (Corchorus olitorius L.) extract suppresses transformation of the aryl hydrocarbon receptor induced by dioxins.,
Food and Chemical Toxicology, Vol.44, No.2, 250-260, 2005.

(要約): Dioxins enter the body mainly through diet and cause the various toxicological effects by binding to the cytosolic aryl hydrocarbon receptor (AhR) followed by its transformation. In recent reports, it has been shown that certain natural compounds suppress AhR transformation in vitro. In this study, we demonstrated that ethanolic extract from molokhia, known as Egyptian spinach, showed the strongest suppressive effect on AhR transformation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in cell-free system using rat hepatic cytosol among 41 kinds of extracts from vegetables and fruits. The molokhia extract also suppressed TCDD-induced AhR transformation in mouse hepatoma Hepa-1c1c7 cells and in intestinal permeability system constructed with human colon adenocarcinoma Caco-2 cells and human hepatoma HepG2 cells. Moreover, oral administration of the molokhia extract (100mg/kg body weight) decreased 3-methylcholanthrene-induced AhR transformation to the control level by inhibiting translocation of the AhR from cytosol into the nucleus in the liver of rats. The molokhia extract-administered rat liver showed a tolerance to TCDD-induced AhR transformation by ex vivo experiment. These results indicate that molokhia is an attractive food for isolation and identification of a natural antagonist for the AhR.
(キーワード): Animals / Blotting, Western / Cell Line, Tumor / Cell Lineage / Cell Nucleus / Cell-Free System / Corchorus / Cytochrome P-450 CYP1A1 / Cytosol / ダイオキシン類 (dioxins) / Enzyme-Linked Immunosorbent Assay / Ethanol / Humans / Male / Mice / Permeability / Plant Extracts / Rats / Rats, Sprague-Dawley / Receptors, Aryl Hydrocarbon / Solvents / Teratogens / Tetrachlorodibenzodioxin / Transformation, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.fct.2005.07.007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16115717; ● Search Scopus @ Elsevier (PMID): 16115717; ● Search Scopus @ Elsevier (DOI): 10.1016/j.fct.2005.07.007

(DOI: 10.1016/j.fct.2005.07.007, PubMed: 16115717) Rie Mukai, Itsuko Fukuda, Keizo Hosokawa, Shin Nishiumi, Atsushi Kaneko and Hitoshi Ashida :
Anthocyans fail to suppress transformation of aryl hydrocarbon receptor induced by dioxin.,
Bioscience, Biotechnology, and Biochemistry, Vol.69, No.5, 896-903, 2005.

(要約): Dioxins induce adverse effects through transformation of the cytosolic aryl hydrocarbon receptor (AhR). Our previous study found that flavones and flavonols at dietary levels suppress AhR transformation. In the present study, we investigated whether 20 anthocyans dissolved in trifluoroacetic acid (TFA)-MeOH suppressed AhR transformation in a cell-free system and in Hepa-1c1c7 cells. Although four compounds at 50 muM suppressed 0.1 nM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AhR transformation and their effects were dose-dependent in the cell-free system, they were ineffective at 0.5 muM, which is close to physiological concentration. Moreover, no anthocyan at 50 muM tested here suppressed 0.1 nM TCDD-induced AhR transformation in Hepa-1c1c7 cells. We also confirmed that protocatechuic acid and related compounds, which are possible metabolites of anthocyans, did not affect the transformation in the cell-free system. It is concluded that anthocyans are not suitable candidates for protection from dioxin toxicity.
(キーワード): Animals / Anthocyanins / Cell Line / Cell-Free System / Mice / 分子構造 (molecular structure) / Receptors, Aryl Hydrocarbon / Tetrachlorodibenzodioxin
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.69.896
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15914907; ● Search Scopus @ Elsevier (PMID): 15914907; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.69.896

(DOI: 10.1271/bbb.69.896, PubMed: 15914907) Itsuko Fukuda, Shin Nishiumi, Yoshiyuki Yabushita, Rie Mukai, Rie Kodoi, Kaoru Hashizume, Masashi Mizuno, Yutaka Hatanaka and Hitoshi Ashida :
A new southwestern chemistry-based ELISA for detection of aryl hydrocarbon receptor transformation: application to the screening of its receptor agonists and antagonists.,
Journal of Immunological Methods, Vol.287, No.1-2, 187-201, 2004.

(要約): Halogenated aromatic hydrocarbons (HAHs), such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), produce a wide variety of biological and toxic effects mainly through the aryl hydrocarbon receptor (AhR)-dependent mechanism. After the binding of HAHs, the AhR subsequently transforms its form in order to interact with a specific DNA sequence, the dioxin responsive element (DRE). Thus, detection of the transformed AhR is a target for estimation of the biological and toxic potency of ligands. In this study, we have developed a simple method for quantitative assessment of the transformation state of AhR based on an enzyme-linked immunosorbent assay (ELISA) combined with southwestern chemistry technique (SW-ELISA) that detects the complex of transformed AhR:fluorescein isothiocyanate (FITC)-labeled DRE probe. SW-ELISA has shown the response to HAHs including TCDD and other known agonists in a dose-dependent manner. In the case of TCDD, SW-ELISA has revealed a minimum detection limit (MDL) of 2 pM (0.026 pg/assay), a median effective concentration (EC(50)) value of 0.125 nM (1.6 pg/assay), and a maximum response at 10 nM (129 pg/assay). Furthermore, SW-ELISA provides the confirmation that flavonoids, the potent antagonists for AhR as reported previously, show the inhibitory effects on TCDD-induced AhR transformation. These results indicate that SW-ELISA is a new and straightforward method for the detection of AhR transformation and will be useful in screening of agonists or antagonists for AhR.
(キーワード): Animals / Antibodies / Blotting, Southwestern / Enzyme-Linked Immunosorbent Assay / Humans / Hydrocarbons, Halogenated / Polycyclic Hydrocarbons, Aromatic / Receptors, Aryl Hydrocarbon / Sensitivity and Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jim.2004.02.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15099767; ● CiNii @ 国立情報学研究所 (CRID): 1360574094811615232; ● Search Scopus @ Elsevier (PMID): 15099767; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jim.2004.02.003

(DOI: 10.1016/j.jim.2004.02.003, PubMed: 15099767, CiNii: 1360574094811615232) Rie Mukai, Itsuko Fukuda, Shin Nishiumi, Keizo Hosokawa, Kazuki Kanazawa and Hitoshi Ashida :
Anthocyan does not suppress transformation of aryl hydrocarbon receptor induced by dioxin.,
BioFactors, Vol.21, No.1-4, 371-373, 2004.

(要約): Dioxins cause a variety of toxic effects through transformation of a cytosolic aryl hydrocarbon receptor (AhR). We have previously demonstrated that certain natural flavones and flavonols at the dietary levels suppress AhR transformation. In this study, we investigated whether 5 anthocyanidins, 15 anthocyanins, and protocatechuic acid suppress AhR transformation in mouse hepatoma Hepa-1c1c7 cells. All the compounds tested here at 5 microM unexpectedly failed to suppress the transformation induced by 0.1 nM TCDD, indicating that anthocyan does not have a potential to prevent dioxin toxicity.
(キーワード): Animals / Anthocyanins / Carcinoma, Hepatocellular / Cell Line, Tumor / Hydroxybenzoates / Liver Neoplasms / Mice / Plant Extracts / Receptors, Aryl Hydrocarbon / Tetrachlorodibenzodioxin
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15630228; ● Search Scopus @ Elsevier (PMID): 15630228

(PubMed: 15630228)

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A Ishisaka, N Fujiwara, Rie Mukai, M Nishikawa, S Ikushiro and A Murakami :
Flavonoids in breast milk and their absorption, metabolism, and bioactivity in infants.,
Bioscience, Biotechnology, and Biochemistry, Oct. 2024. Rie Mukai :
Prenylation enhances the biological activity of dietary flavonoids by altering their bioavailability.,
Bioscience, Biotechnology, and Biochemistry, Vol.82, No.2, 207-215, Jan. 2018.

(要約): Flavonoids are distributed across the plant kingdom and have attracted substantial attention owing to their potential benefits for human health. Several studies have demonstrated that flavonoids prenylation enhances various biological activities, suggesting an attractive tool for developing functional foods. This review provides an overview of the current knowledge on how prenylation influences the biological activity and bioavailability of flavonoids. The enhancement effect of prenylation on the biological activities of dietary flavonoids in mammals was demonstrated by comparing the effect of 8-prenyl naringenin (8PN) with that of parent naringenin in the prevention of disuse muscle atrophy in mice. This enhancement results from higher muscular accumulation of 8PN than naringenin. As to bioavailability, despite the lower absorption of 8-prenyl quercetin (8PQ) compared with quercetin, higher 8PQ accumulation was found in the liver and kidney. These data imply that prenylation interferes with the elimination of flavonoids from tissues.
(キーワード): Animals / Biological Availability / Diet / フラボノイド (flavonoids) / Humans / Intestinal Absorption / Prenylation / Tissue Distribution
(徳島大学機関リポジトリ): ● Metadata: 112454
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09168451.2017.1415750
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29307271; ● Search Scopus @ Elsevier (PMID): 29307271; ● Search Scopus @ Elsevier (DOI): 10.1080/09168451.2017.1415750

(徳島大学機関リポジトリ: 112454, DOI: 10.1080/09168451.2017.1415750, PubMed: 29307271) Kyuichi Kawabata, Rie Mukai and Akari Ishisaka :
Quercetin and related polyphenols: new insights and implications for their bioactivity and bioavailability.,
Food & Function, Vol.6, No.5, 1399-1417, May 2015.

(要約): The physiological functions and bioavailability of flavonoids have been widely investigated since their bioactivities were identified about 80 years ago. Quercetin is a typical flavonoid ubiquitously contained in vegetables and fruits with several biological effects demonstrated in vitro and in vivo including antioxidative, anti-inflammatory, anticancer, and antidiabetic activities. After the ingestion of vegetables and fruits, quercetin glycosides are metabolized, absorbed, and circulated as types of conjugates in the blood. Thereafter, quercetin-3-O--D-glucuronide (Q3GA), a major metabolite of quercetin, is distributed throughout the body where it may exert beneficial functions in target tissues. Hydrophilic Q3GA has been found to be deconjugated into hydrophobic quercetin aglycone at injured sites which, in turn, may improve the pathological conditions. This review presents updated information on the biological aspects and mechanisms of action of quercetin and its related polyphenols. In particular, new insights into their beneficial health effects on the brain, blood vessels, muscle, and intestine will be discussed.
(キーワード): Animals / Anti-Inflammatory Agents / Antioxidants / Biological Availability / Humans / Polyphenols / Quercetin
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/c4fo01178c
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25761771; ● Search Scopus @ Elsevier (PMID): 25761771; ● Search Scopus @ Elsevier (DOI): 10.1039/c4fo01178c

(DOI: 10.1039/c4fo01178c, PubMed: 25761771) Junji Terao and Rie Mukai :
Prenylation modulates the bioavailability and bioaccumulation of dietary flavonoids.,
Archives of Biochemistry and Biophysics, Vol.559, 12-16, Apr. 2014.

(要約): Prenylflavonoids are distributed widely in the plant kingdom and have attracted appreciable attention because of their potential benefits for human health. Prenylation may be a promising tool for applying the biological functions of flavonoids to clinical uses. The bioavailability and bioaccumulation of prenylflavonoids have not been clarified, but extensive studies have been accomplished on their biological functions. This review provides current knowledge on the bioavailability of prenylflavonoids, including their absorption and metabolism in the intestine, as well as their bioaccumulation in specific tissues. Despite higher uptake into epithelial cells of the digestive tract, the bioavailability of single-dose prenylflavonoids seems to be lower than that of the parent flavonoids. Efflux from epithelial cells to the blood circulation is likely to be restricted by prenyl groups, resulting in insufficient increase in the plasma concentration. Rodent studies have revealed that prenylation enhances accumulation of naringenin in muscle tissue after long-term feeding; and that prenylation accelerates accumulation of quercetin in liver tissue. Efflux from hepatocytes to blood and enterohepatic circulations may be restricted by prenyl groups, thereby promoting slow excretion of prenylflavonoids from the blood circulation and efficient uptake to tissues. The hepatotoxicity and other deleterious effects, taken together with beneficial effects, should be considered because unexpectedly high accumulation may occur in some tissues after long-term supplementation.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.abb.2014.04.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24736381; ● Search Scopus @ Elsevier (PMID): 24736381; ● Search Scopus @ Elsevier (DOI): 10.1016/j.abb.2014.04.002

(DOI: 10.1016/j.abb.2014.04.002, PubMed: 24736381) Rie Mukai and Junji Terao :
Role of dietary flavonoids in oxidative stress and prevention of muscle atrophy,
The Journal of Physical Fitness and Sports Medicine, Vol.2, No.4, 385-392, Nov. 2013.

(要約): Functional foods for the prevention of disuse muscle atrophy (DMA) are expected to improve the quality of life (QoL) of bedridden people. Ubiquitin ligases targeting muscle protein degradation, atrogin-1 and muscle-specific ring finger protein (MuRF-1), are critical in the degradation of muscle protein, and oxidative stress induced by mitochondrial dysfunction seems to be involved in muscle atrophy. Dietary antioxidants that attenuate the oxidative stress in skeletal muscle are strong candidates as food ingredients for preventing DMA. The antioxidative flavonoid quercetin was found to prevent DMA by attenuating the induction of atrogin-1/MuRF-1 in mice undertaking the tail suspension test. Several studies revealed that dietary quercetin accumulates in skeletal muscle after metabolic conjugation during absorption. There are many arguments that antioxidant activity is essential for dietary flavonoids to exert their preventive effects, but modulation of the IGF-1 signaling pathway is definitively involved in the mechanism of prevention. Nevertheless, dietary flavonoids (including quercetin) may be potential food factors in the prevention of muscle atrophy. Dietary flavonoids are expected to prevent DMA by attenuating oxidative stress derived from mitochondrial dysfunction.
(キーワード): flavonoid / antioxidant / oxidative stress / disuse muscle atrophy / ubiquitin ligase
(出版サイトへのリンク): ● Publication site (DOI): 10.7600/jpfsm.2.385
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680392121984; ● Search Scopus @ Elsevier (DOI): 10.7600/jpfsm.2.385

(DOI: 10.7600/jpfsm.2.385, CiNii: 1390282680392121984) Shin Nishiumi, Shingo Miyamoto, Kyuichi Kawabata, Kohta Ohnishi, Rie Mukai, Akira Murakami, Hitoshi Ashida and Junji Terao :
Dietary flavonoids as cancer-preventive and therapeutic biofactors.,
Frontiers in Bioscience (Scholar edition), Vol.3, 1332-1362, Jun. 2011.

(要約): Flavonoids are present in many plants, and hence, in foods and ingredients derived from them. These polyphenolic compounds have attracted renewed attention as potential anticarcinogens, and the molecular mechanisms of their anticarcinogenic effects and their bioavailability have been extensively explored. In this review, we focus on the major dietary flavonoids; flavones, flavonols, and flavan-3-ols (catechins), and evaluate their roles in cancer prevention. After absorption with or without metabolic conjugation, flavonoids are transported to target organs where they exert their anticarcinogenic activity. The molecular mechanisms of the anticarcinogenic effects of flavonoids include their antagonistic effect on the aryl hydrocarbon receptor (AhR), and regulation of phase I and II drug metabolizing enzymes and phase III transporters. Experimental evidence suggests that flavonoids modulate signal transduction pathways at each stage of carcinogenesis. The interactions between flavonoids and biomolecules in vivo must be investigated in detail to identify specific targets. In addition, the potential side effects should be considered when flavonoid supplements are used for cancer prevention. Therefore, the use of flavonoids as chemopreventive agents should be further investigated to establish safe levels of flavonoid intake.
(キーワード): Absorption / Animals / Dietary Supplements / Enzyme Activation / フラボノイド (flavonoids) / Humans / Mice / 分子構造 (molecular structure) / Neoplasms / Receptors, Aryl Hydrocarbon / シグナル伝達 (signal transduction)
(出版サイトへのリンク): ● Publication site (DOI): 10.2741/229
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21622274; ● Search Scopus @ Elsevier (PMID): 21622274; ● Search Scopus @ Elsevier (DOI): 10.2741/229

(DOI: 10.2741/229, PubMed: 21622274)

講演・発表

Rie Mukai, Goto-Inoue Naoko, Ukawa Yuichi, Kohara Akiko, Oe Kenichi and Junji Terao :
Tissue distribution of 8-prenylnaringnein in mice,
Boston, MA, USA, Oct. 2024. Ishisaka Akari, Fujiwara Nao, Rie Mukai, nishikawa Miyu, Ikushiro Shinichi and Murakami Akira :
Effects of quercetin transfer to breast milk on physiological functionality in infant mice.,
Boston, MA, USA, Oct. 2024. Koichi Ohashi, Ryutaro Sogawa, Nene Hashimura, Rie Mukai and Retsuo Kawakami :
Increased Polyphenol Content of Harvested Onions Irradiated with Low-Temperature Air Plasma Jet at Quasi-Atmospheric Pressure,
Proceedings of International Symposium of Dry Process 2023, 171-172, Nagoya, Nov. 2023. Fujiwara Nao, Rie Mukai, Murakami Akira and Ishisaka Akari :
Infants may be exposed to quercetin and its metabolites via breast and formula milk.,
Intrernational conference on Nutrition, Tokyo, Dec. 2022. Matsumura Takumi, Sogawa Ryutaro, Hashimura Nene, Ohashi Koichi, Rie Mukai and Retsuo Kawakami :
Effects of Quasi-Atmospheric-Pressure Low-Temperature Air Plasma Jet Irradiation on Increasing Minerals in Fresh Food,
Proceedings of International Symposium of Dry Process 2022, 123-124, Osaka, Nov. 2022. Ishisakai Akari, Fujiwara Nao, Rie Mukai and Murakam Akira :
Quercetin ingested by maternal mice may be transferred to newborn mice via breast milk,
The 10th International Conference on Polyphenols and Health, London, UK, Apr. 2022. Hitomi Okuyama, Mayumi Ikeda, Yu Ishima, Miyu Nishikawa, Shinichi Ikushiro and Rie Mukai :
Binding profile of quercetin and its derivatives to human serum albumin measured by multiplex drug-site mapping,
ICoFF2019/ISNFF2019/ICPH2019, Kobe, Nov. 2019. Manamu Kitami, Rie Mukai, Miyu Nishikawa, Keisuke Fukaya, Daisuke Urabe, Toshiyuki Sakaki and Shinichi Ilushiro :
Whole cell-dependent preparation of 8-prenylnaringenin glucuronides using UDP-glucuronosyltransferase expressing yeast.,
ICoFF2019/ISNFF2019/ICPH2019, Kobe, Nov. 2019. Takahito Takase, Naoyuki Kobayashi, Takashi Inoue, Yoichi Tsuchiya and Rie Mukai :
Preventive effect of dietary lemon peel extract containing eriocitrin on disuse muscle atrophy in denervated mice,
ICoFF2019/ISNFF2019/ICPH2019, Kobe, Nov. 2019. 向井理恵, Hisao Nemoto, 寺尾純二 :
Estrogenic activity of 8-renylnaringenin improve skeletal muscle regeneration from disuse muscle atrophy by activating IGF-I/PI3K/Akt pathway,
ICoFF2019/ISNFF2019/ICPH2019, 神戸, 2019年11月. Hiroaki Kanouchi, Rie Mukai and De-Xing Hou :
Comparison of dietary meat types on muscle atrophy and recovery caused by immobilization-induced disuse muscle atrophy.,
Vancouver, Jul. 2018. Akari Kondo, Chiemi Kamada, Rie Mukai and Junji Terao :
Effect of Quercetin and Its Metabolite on Hydrogen Peroxide-Induced Phosphorylation of Caveolin-1 in Endothelial Cells,
The 6th International Conference on Food Factors: Bioconvergence for Food Function, Soul, Republic of Korea, Nov. 2015. Asami Iwanaka, Mamiko Soga, Eiichi Kotake-Nara, Akihito Nagao, Rie Mukai, Akira Takahashi and Junji Terao :
Effect of Fucoxanthin and Neoxanthin on Blue Light-Emitting Diode-Irradiated Photosensitized Oxidation in Liposomal Membranes andMouse Fibroblast Cells,
The 6th International Conference on Food Factors: Bioconvergence for Food Function, Soul, Republic of Korea, Nov. 2015. Hiromi Shono, Hideyuki Saito, Shinya Sato, Tomoyuki Kawamura, Hisao Nemoto, Junji Terao and Rie Mukai :
A signal transduction pathway in prenylquercetin-induced heme oxygenase-1 expression in vascular endothelium cells,
The 6th International Conference on Food Factors: Bioconvergence for Food Function, Soul, Republic of Korea, Nov. 2015. Rie Mukai, Onishi Asami, Fukuda Takashi, Furusawa Mutsuki, Ashitani Hiroaki, Koyama Toshiyuki, Usami-Krank Yoko and Junji Terao :
Epi-catechin Gallate Selectively Accumulates in Blood Plasma after Continuous Intake of Tea Catechins in Rats and Humans,
The 6th International Conference on Food Factors: Bioconvergence for Food Function, Soul, Republic of Korea, Nov. 2015. Rie Mukai, Hiromi Shono, Shinya Sato, Jeremy Spencer, Tomoyuki Kawamura, Hisao Nemoto and Junji Terao :
Positional isomers of prenylquercetin differently induce hemeoxygenase-1 expression in vascular endothelial cells,
7th International Conference on Polyphenols and Health, Tours, France, Oct. 2015. Lin Yi Pei, 向井理恵, Horikawa Hitomi, 寺尾純二 :
8-Prenylnaringenin enhances the recovery from disuse muscle atrophy of mice with cast immobilization,
12th Asian congress of Nutrition, Yokohama, 2015年5月. Rie Mukai, Naoko Matsui, Takeshi Nikawa and Junji Terao :
Dietary quercetin prevents disuse muscle atrophy of denervated mice through regulation of mitochondrial biogenesis,
12th Asian congress of Nutrition, Yokohama, May 2015. Yauhen Bandaruk, Rie Mukai and Junji Terao :
Role of flavonoids as possible monoamine oxidase-A regulators in the model of serotoninergic neuroblastoma SH-SY5Y cell,
XXVIIth International Conference on Polyphenols &8th Tannin Conference, Nagoya, Sep. 2014. Rie Mukai, Naoko Matsui, Takeshi Nikawa and Junji Terao :
Pre-intake of quercetin slow the progression of disuse muscle atrophy by mitochondrial dysfunction.,
XXVIIth International Conference on Polyphenols &8th Tannin Conference, Nagoya, Sep. 2014. Rie Mukai, Naoko Matsui, N Matsumoto, N.T. Dang, Takeshi Nikawa, Hisao Nemoto and Junji Terao :
Anti-Oxidative Flavonoid suppresses disuse muscle atrophy in denervation mice.,
XXVIIth International Conference on Polyphenols &8th Tannin Conference., 2014. Arisa Ochi, Katsuya Hirasaka, Ayako Maita, Shigetada Kondo, Tomoyuki Kawamura, Hisao Nemoto, Rie Mukai, Junji Terao, Taesik Gwang, Inho Choi and Takeshi Nikawa :
Development of anti-ubiquitination oligopeptide, Cblin:Cbl-b inhibitor that prevents unloading-induced muscle atorophy.,
The 29th International Symposium on Space Technology and Science, Nagoya, Jun. 2013. Yauhen Bandaruk, Rie Mukai and Junji Terao :
Attenuation of monoamine oxidase-A activity and its expression level in the brain as possible mechanism of antidepressant-line action of flavonoids.,
The 17th Biennial Meeting of Society for free radical international, Mar. 2013. Rie Mukai, Horikawa Hitomi, Fujikura Yutaka, Kaeko Murota, Kawamura Tomoyuki, Hisao Nemoto and Junji Terao :
Effect of prenylation on the bioavailability of dietary flavonoids,
The International Society for Nutraceuticals and Functional Foods, Dec. 2012. Rie Mukai, Takeshi Nikawa and Junji Terao :
Antioxidative flavonoid quercetin suppresses disused muscle atrophy,
Free Radical Biology and Medicine, Vol.53, S87, 2012. Tomoyuki Kawamura, Moemi Hayashi, Rie Mukai, Junji Terao and Hisao Nemoto :
Synthesis and Evaluation of C8 Prenylated Flavonoid Suppressed Disused Muscular Atrophy,
the 8th AFMC InternationalMedicinal Chemistry Symposium (AIMECS 11), Nov. 2011. Yutaka Fujikura, Rie Mukai, Tomoyuki Kawamura, Yaeko Mizuguchi, Hisao Nemoto, Kaeko Murota and Junji Terao :
Effect of prenylation on bioavailability and cellular uptake of quercetin aglycone,
Internatinal Conference on Food Factors, Taipei, Nov. 2011. Rie Mukai, Tomoyuki Kawamura, Hisao Nemoto, Takeshi Nikawa, Hironori Yamamoto, Eiji Takeda and Junji Terao :
Flavonoid derivatives prevent disuse muscle atrophy,
Internatinal Conference on Food Factors, Taipei, Nov. 2011. Kohno Shohei, Shigetada Kondo, Katsuya Hirasaka, Ayako Maita, Yuushi Okumura, Rie Mukai, Junji Terao, Akira Higashibata and Takeshi Nikawa :
Regulation of the Gene Expression of Cbl-b Ubiquitin Ligase in Skeletal Muscle During Unloading Conditions.,
7th General Meeting of the International Proteolisis Society, 米国, Oct. 2011. Hironori Yamamoto, Tsuji Mitsuyoshi, Rie Mukai, T Inakuma, Junji Terao and Eiji Takeda :
Dietary quercetin and onion powder could prevent immobilization induced bone loss in rats,
5th International Conference on Polyphenols and Health (ICPH), Barcelona, Oct. 2011. Rie Mukai, Tomoyuki Kawamura, Yutaka Fujikura, Hitomi Horikawa, Hisao Nemoto and Junji Terao :
Bioavailability and muscle atrophy-preventive effect of prenylated flavonoids,
International Conference on Polyphenols and Health, Sitges, Barcelona, Spain, Oct. 2011. Rie Mukai, Takeshi Nikawa, Hisao Nemoto, Hironori Yamamoto, Eiji Takeda, Yoshichika Kawai and Junji Terao :
Disuse muscle atrophy is suppressed by antioxidative flavonoid quercetin,
International Symposium on Free Radical Research: Contribution to Medicine, Kyoto, Jan. 2011. Ashida. Hitoshi, Rie Mukai, Satsu Hideo and Shimizu Makoto :
Suppression of the aryl hydrocarbon receptor-mediated signaling,
International Chemical Congress of Pacific Basin Societies., Honolulu, Hawaii, Dec. 2010. Rie Mukai, Tomoyuki Kawamura, Yutaka Fujikura, Moemi Hayashi, Hisao Nemoto, Takeshi Nikawa, Hironori Yamamoto, Eiji Takeda and Junji Terao :
Disuse muscle atrophy is prevented by flavonoids,
2010 International Chemical Congress of Pacific Basin Societies, Honolulu, Dec. 2010. Tomoyuki Kawamura, Moemi Hayashi, Rie Mukai, Junji Terao and Hisao Nemoto :
Chemical Synthesis of O-Methylated and/or C-Prenylated Flabonoid Analogues,
Pachifichem 2010, Honolulu, Dec. 2010. Rie Mukai, Hitoshi Ashida, Takeshi Nikawa and Junji Terao :
Nuclear accumulation of flavonol aglycone in cultured cells detected with a fluorescence microscope,
4th International Conference on Polyphenol and Health, Harrogate, United Kingdom, Dec. 2009. I Fukuda, Rie Mukai, m Kawase, K Yoshida and H Ashida :
(-)-Epigallocatechin gallate interacts with an aryl hydrocarbon receptor complex.,
Proceedings of The 3rd International Conference on O-CHA (Tea) culture and Science (2007)., 2007. 谷内滉, 向井理恵, 川上烈生 :
タマネギ中ポリフェノールへの大気圧低温空気プラズマジェット照射効果,
2024年第85回応用物理学会秋季学術講演会, 07-162, 2024年9月. 石坂朱里, 藤原なお, 向井理恵, 西川美宇, 生城真一, 村上明 :
授乳期のフラボノイド摂取が乳児の生体機能に与える影響,
日本農芸化学会 2024年度東京大会, 2024年3月. 立石晟菜, 韓俊文, 棟方涼介, 矢埼一史, 古賀武尊, 田井章博, 向井理恵 :
プレニル化がケンフェロールの抗酸化性に及ぼす影響,
日本農芸化学会 2024年度東京大会, 2024年3月. 藤原なお, 向井理恵, 西川美宇, 生城真一, 村上明, 石坂朱里 :
乳汁への用量依存的なケルセチン移行が乳仔の生理機能性に与える影響,
第28回日本フードファクター学会学術集会無礼講学会におけるブレイクスルー, 2023年11月. 立石晟菜, 韓俊文, 棟方涼介, 矢埼一史, 古賀武尊, 田井章博, 向井理恵 :
プレニルケンフェロールの抗酸化性の比較,
第28回日本フードファクター学会学術集会無礼講学会におけるブレイクスルー, 2023年11月. 庄野陸, 韓俊文, 棟方涼介, 田井章博, 矢﨑一史, 古賀武尊, 向井理恵 :
プレニルフラボノイドの抗アレルギー作用,
学会創立100周年記念日本農芸学会2023年度中四国・西日本支部合同大会, 2023年9月. 大橋孝一, 十川竜太朗, 橋村寧々, 向井理恵, 川上烈生 :
大気圧低温空気プラズマジェット照射後のタマネギのポリフェノール含有量の増加現象,
2023年第84回応用物理学会秋季学術講演会, 07-052, 2023年9月. 植野美彦, 関陽介, 服部武文, 田端厚之, 向井理恵, 岡直宏, 宇都義浩 :
B学部における学校推薦型選抜Ⅰ地方創生型(地域産業振興枠)の設計と実施 ―入試業務効率化に向けた新たな取り組みを踏まえて―,
令和5年度全国大学入学者選抜研究連絡協議会大会(第18回), 2023年5月. 橋村寧々, 十川竜太朗, 堤理恵, 瀬川博子, 小原亜希子, 大江健一, 卯川裕一, 向井理恵 :
ホップ由来フラボノイドによる骨格筋でのアミノ酸取り込み促進作用,
第77回日本栄養・食糧学会大会, 2023年5月. 橋村寧々, 十川竜太朗, 松廣美優, 松村拓海, 大橋孝一, 川上烈生, 向井理恵 :
準大気圧低温空気プラズマジェットを活用したタマネギ中ポリフェノールの増産,
日本農芸化学会2023年度大会, 2023年3月. 小西冴季, 竹上菜緒, 志内哲也, 向井理恵 :
高脂肪誘導性肥満マウスにおいて8-プレニルナリンゲニンが及ぼす代謝変化,
支部創立20周年記念日本農芸化学会中四国支部第64回講演会(例会), 2023年1月. 平田愛佳, 佐藤匠, 増田尚輝, 姚陳娟, 向井理恵, 渡辺崇人, 三戸太郎, 赤松徹也 :
コオロギ食が唾液腺機能に及ぼす効果の検証,
第1回唾液ケア研究会学術集会, 2022年11月. 藤原なお, 向井理恵, 生城真一, 村上明, 石坂朱里 :
ケルセチン摂取後の母仔マウスにおけるケルセチン代謝物の解析,
第27回日本フードファクター学会, 2022年10月. 十川竜太朗, 橋村寧々, 田中寛人, 堤理恵, 大江健一, 卯川裕一, 向井理恵 :
8-プレニルナリンゲニンが骨格筋のアミノ酸動態に及ぼす影響,
第27回日本フードファクター学会, 2022年10月. 竹上菜緒, 小西冴季, 山下陽子, 志内哲也, 卯川裕一, 大江健一, 向井理恵 :
高脂肪食負荷による組織機能破綻に対する8-プレニルナリンゲニンの効果,
2022年度中四国支部大会(第63回講演会), 2022年9月. 松村拓海, 十川竜太朗, 橋村寧々, 大橋孝一, 向井理恵, 川上烈生 :
準大気圧低温空気プラズマジェット照射による食品機能性成分増量効果,
2022年第83回応用物理学会秋季学術講演会, 07-042, 2022年9月. 平田愛佳, 佐藤匠, 田中寛人, 増田尚輝, 姚陳娟, 向井理恵, 長谷川敬展, 吉村弘, 渡辺崇人, 三戸太郎, 赤松徹也 :
食品成分が唾液腺機能に及ぼす影響,
第34回唾液腺談話会, 2022年9月. 藤原なお, 向井理恵, 村上明, 石坂朱里 :
乳汁を介したケルセチン移行が乳児に及ぼす生理的意義の究明,
第25回フードサイエンスフォーラム, 2022年9月. 額惠理香, 沖津真美, 向井理恵, 川畑球一, 寺尾純二 :
タマネギケルセチン配糖体の加水分解反応に対する調理加工の影響,
第76回日本栄養・食糧学会, 2022年6月. 藤原なお, 向井理恵, 村上明, 石坂朱里 :
4G03-10乳汁中ケルセチンの摂取が新生仔マウスの薬物代謝機構に与える影響,
日本農芸化学会2022年度大会, 2022年3月. 増田尚輝, 阪本鷹行, 西海信, 渡辺崇人, 櫻谷英治, 三戸太郎, 向井理恵 :
食用昆虫の摂取が実験動物の代謝に及ぼす影響,
日本農芸化学会2022年度大会, 2022年3月. 向井理恵 :
骨格筋を標的とした機能性食品の開発に向けた基盤研究,
ダイバーシティ推進研究交流会オンライン2021(愛媛大学オンライン), 2022年2月. 田中寛人, 堤理恵, 大江健一, 卯川裕一, 向井理恵 :
廃用性筋萎縮からの回復を促進する8-プレニルナリンゲニンがアミノ酸動態に与える影響について,
支部創立20周年記念日本農芸化学会中四国支部第61回支部講演会(例会), 2022年1月. 増田尚輝, 阪本鷹行, 西海信, 渡辺崇人, 櫻谷英治, 三戸太郎, 向井理恵 :
食用昆虫の脂質組成分析と実験動物への影響,
2021年度第3回脂質駆動学術産業創生研究部会講演会, 2021年12月. 藤原なお, 向井理恵, 村上明, 石坂朱里 :
乳児の薬物代謝機構に対する母乳中フラボノイドの影響,
第26回日本フードファクター学会学術集会, 2021年11月. 奥山仁美, 内村望空, 坂尾こず枝, 池田真由美, 異島優, 西川美宇, 生城真一, 向井理恵 :
ヒト血清アルブミンに対するケルセチンの結合特性が機能評価実験に及ぼす影響,
第26回日本フードファクター学会学術集会, 2021年11月. 近藤あかり, 酒井徹, 寺尾純二, 向井理恵 :
血管内皮細胞における過酸化水素誘導性カベオリン-1リン酸化に対するケルセチンの抑制効果,
第54回日本栄養・食糧学会中国・四国支部大会第7回日本栄養改善学会四国支部学術総会合同大会(オンライン開催), 2021年10月. 平田愛佳, 佐藤匠, 田中寛人, 小西冴季, 姚陳娟, 向井理恵, 赤松徹也 :
マウス唾液腺AQP5発現に及ぼす高脂肪食および低タンパク質食摂取の影響,
第63回歯科基礎医学会学術大会, 2021年10月. 田中寛人, 堤理恵, 向井理恵 :
骨格筋量の調節に寄与するポリフェノールがアミノ酸動態に与える影響について,
第6回メタボローム解析シンポジウム(徳島大学医学部医科栄養学科メタボローム解析室), 2021年10月. 平田愛佳, 佐藤匠, 田中寛人, 小西冴季, 姚陳娟, 向井理恵, 赤松徹也 :
高脂肪食や低タンパク質食が引き起こすマウス唾液腺AQP5発現・局在変化,
第75回日本栄養・食糧学会大会, 2021年7月. 秦菜摘, 向井理恵 :
経口投与したイソキサントフモールの臓器分布特性,
第75回日本栄養・食糧学会大会, 2021年7月. 山田詩奈, 中島賢則, 卯川裕一, 大江健一, 向井理恵, 芦田均, 山下陽子 :
8- プレニルナリンゲニンの肥満を抑制する効果,
第75回日本栄養・食糧学会大会, 2021年7月. 吉田真生, 田井章博, 向井理恵 :
ケルセチンのプレニル化による抗酸化特性の変化,
日本農芸化学会2021年度大会, 2021年3月. 山田詩奈, 中島賢則, 卯川裕一, 大江健一, 向井理恵 :
8- プレニルナリンゲニンの肥満抑制効果,
日本農芸化学会2021年度大会, 2021年3月. 田中寛人, 西海信, 堤理恵, 向井理恵 :
筋タンパク質合成時にプレニルナリンゲニンが引き起こす骨格筋内代謝物の変動,
第13回日本ポリフェノール学会・第16回日本カテキン学会合同学術集会, 2019年11月. 高瀬貴仁, 小林直之, 井上孝司, 土屋陽一, 向井理恵 :
レモン果皮抽出物の筋委縮予防効果について,
日本食品科学工学第66回大会, 2019年8月. 白井郁太, 藤野翔多, 向井理恵, 辻明彦, 山本圭 :
骨格筋におけるリン脂質代謝系の網羅的脂質メタボローム解析．第41回日本分子生物学会年会,
第41回日本分子生物学会, 2018年11月. 向井理恵 :
食品機能学によるプレニルフラボノイドの特性解明,
日本農芸化学会中四国支部第50回記念講演会, 2018年1月. 向井理恵 :
食品機能学によるプレニルフラボノイドの特性解明,
日本農芸化学会2017年度大会, 2017年3月. 向井理恵 :
フラボノイドのプレニル化による生体利用性の変化と機能性の増強,
第 23 回若手シンポジウム (第8回農芸化学の未来開拓セミナー), 2016年5月. 鎌田智英実, 近藤あかり, 向井理恵, 寺尾純二 :
血管内皮細胞における酸化LDLおよびLysoPCによるcaveolin-1発現に対するケルセチンおよび代謝物の効果,
第70回日本栄養・食糧学会大会, 2016年5月. 鎌田智英実, 近藤あかり, 向井理恵, 寺尾純二 :
caveolin-1発現に対するケルセチンおよびαトコフェロールの効果,
第27回ビタミンE研究会, 2016年1月. 向井理恵 :
植物ポリフェノールによる筋萎縮予防について,
四国オープンイノベーションワークショップ, 2015年11月. 近藤あかり, 鎌田智英実, 向井理恵, 寺尾純二 :
血管内皮細胞における酸化ストレス誘導のcaveolin-1リン酸化に対するケルセチン及びその代謝物の効果,
日本農芸学会2015年度中国四国・西日本支部合同大会, 2015年9月. 向井理恵 :
生体内ケルセチン抱合体の臓器での脱抱合に関する研究,
第15回AOB(Antioxidant Biofactor)研究会, 2015年7月. 林珮儀, 齋藤豪紀, 河合知志, 根本尚夫, 寺尾純二, 向井理恵 :
Suppressive effect of prenylflavonoids on atrogin-1 expression in C2C12 mouse myotubes.,
日本農芸化学会中四国支部第42回講演会, 2015年6月. 庄野裕美, 齋藤豪紀, 佐藤伸也, 河合知志, 根本尚夫, 寺尾純二, 向井理恵 :
血管内皮におかるプレニルケルセチンによるヘムオキシゲナーゼ1誘導に関与するシグナル経路の検討,
日本農芸化学会中四国支部第42回講演会, 2015年6月. 向井理恵, 鈴木里穂, 田中義章, 嶺川祥子, 河合慶親, 寺尾純二 :
ケルセチン抱合体代謝物に対する腎臓の脱抱合化反応の解析,
日本農芸化学会2016年度大会, 2015年3月. 高橋正子, 庄野裕美, 向井理恵, 井出晋太郎, 朝武宗明, 濵田康弘, 寺尾純二 :
タマネギケルセチンの生体利用性に対する食べ合わせの効果-同時摂取食材がケルセチン血漿濃度に与える影響-,
日本農芸化学会2015年度大会, 2015年3月. 福田貴史, 向井理恵, 大西愛紗美, 芦屋浩明, 小山寿之, 宇佐美クランク陽子, 寺尾純二 :
エピカテキンガレートとエピガロカテキンガレート摂取後の血中濃度の比較,
第47回日本栄養・食糧学会中国・四国支部大会, 2014年11月. 向井理恵, 松井直子, 二川健, 寺尾純二 :
ケルセチンはミトコンドリアの機能を保つことで廃用性筋萎縮を予防する,
第8回日本ポリフェノール学会学術大会, 2014年8月. 向井理恵 :
抗酸化フラボノイドの抗筋委縮効果,
第14回AOB(Antioxidant Biofactor)研究会, 2014年6月. 庄野裕美, 片岡美樹, 西村沙紀, 坂東紀子, 山岸喬, 井出晋太郎, 朝武宗明, 中村俊之, 向井理恵, 寺尾純二 :
含有食品素材の調理加工がケルセチン・イソフラボンの生体利用性に及ぼす影響の評価,
第68回日本栄養・食料学会大会, 2014年5月. 堀川瞳, 向井理恵, 河村知志, 根本尚夫, 二川健, 寺尾純二 :
プレニルナリンゲニンは廃用性筋萎縮を予防・改善するか?,
第30回臨床フリーラジカル会議, 2013年12月. 堀川瞳, 向井理恵, 河村知志, 根本尚夫, 二川健, 寺尾純二 :
プレニルナリンゲニンは廃用性筋萎縮からの筋量回復を促進する,
第18回日本フードファクター学会学術集会, 2013年11月. 向井理恵, 藤倉温, 室田佳恵子, 上原万里子, 嶺川祥子, 松井直子, 河村知志, 根本尚夫, 寺尾純二 :
プレニルフラボノイドの生体利用性―プレニル化がケルセチンの腸管吸収と組織蓄積に与える影響の解析,
第7回日本ポリフェノール学会，, 2013年8月. 水口八重子, 向井理恵, 寺尾純二 :
抗酸化フラボノイドの廃用性筋萎縮予防効果，培養細胞による評価,
第13回AOB研究会, 2013年7月. 松井直子, 向井理恵, 候徳興, 河村知志, 根本尚夫, 二川健, 寺尾純二 :
酸化ストレス上昇を伴う廃用性筋萎縮に対する抗酸化プレニルケルセチンの効果,
第67回本栄養食糧学会, 2013年5月. 嶺川祥子, 向井理恵, 藤倉温, 生城真一, 榊利之, 室田佳恵子, 河村知志, 根本尚夫, 寺尾純二 :
プレニル基付加がケルセチンのグルクロン酸抱合とABCトランスポーターによる排出に及ぼす影響,
第67階日本栄養食糧学会, 2013年5月. Yauhen Bandaruk, 向井理恵, 寺尾純二 :
Accumulation of flavonoids quercetin and luteolin in SH-SY5Y cells and their effects on Monoamine oxidase-A,
日本農芸化学会2014年度大会, 2013年3月. 向井理恵 :
プレニルフラボノイドによる廃用性筋萎縮予防の可能性,
第5回レドックス・ライフイノベーションシンポジウム, 2013年3月. 水口八重子, 向井理恵, 寺尾純二 :
筋管細胞における遊離脂肪酸ヒドロペルオキシド誘導ユビキチンリガーゼ発現に対するケルセチンの抑制作用,
第17回日本フードファクター学会学術集会第9回日本カテキン学会総会合同大会 2012, 2012年11月. 堀川瞳, 向井理恵, 藤倉温, 河村知志, 根本尚夫, 二川健, 寺尾純二 :
坐骨神経切除マウスにおける8-プレニルナリンゲニンの廃用性筋萎縮抑制効果,
第17回日本フードファクター学会学術集会第9回日本カテキン学会総会合同大会 2012, 2012年11月. 向井理恵, 嶺川祥子, 藤倉温, 生城真一, 榊利之, 室田佳恵子, 河村知志, 根本尚夫, 寺尾純二 :
プレニル基の導入がケルセチンの生体利用性に与える影響,
第17回日本フードファクター学会学術集会第9回日本カテキン学会総会合同大会, 2012年11月. 向井理恵 :
植物フラボノイドの高機能化戦略 -- 筋萎縮予防を中心に -- :招待講演,
日本農芸化学会中四国支部第14回若手シンポジウム「生物資源化学の新たな展開」, 2012年10月. Yauhen Bandaruk, 向井理恵, 寺尾純二 :
脳内セロトニン代謝とフラボノイド:マウス脳ミトコンドリアのmonoamine oxidase-A活性阻害の評価,
第12回AOB研究会, 2012年6月. 向井理恵, 堀川瞳, 河村知志, 根本尚夫, 二川健, 寺尾純二 :
プレニル化フラボノイドによる廃用性筋萎縮の予防,
第66回日本栄養・食糧学会大会, 2012年5月. 河村知志, 向井理恵, 林萠未, 寺尾純二, 根本尚夫 :
廃用性筋萎縮抑制作用を有するフラボノイドのC-プレニル化と活性評価,
第132回日本薬学会, 2012年3月. Bandaruk Yauhen, Nakamura Toshiyuki, Rie Mukai and Terao Junji :
Evaluation of the inhibitory effect of flavonols and catechins on monoamine,
日本農芸化学会2012年度大会, Mar. 2012. 藤倉温, 向井理恵, 河村知志, 室田佳恵子, 根本尚夫, 寺尾純二 :
筋萎縮抑制効果を示すプレニル化ケルセチンの生体利用性,
日本農芸化学会中四国支部大会, 2012年1月. 河村知志, 林萠未, 向井理恵, 寺尾純二, 根本尚夫 :
フラボノイドの効率的C-プレニル化法の開発と合成への応用,
第37回反応と合成の進歩シンポジウム, 2011年11月. 向井理恵, 寺尾純二 :
抗酸化フラボノイドの廃用性筋萎縮予防効果―モデル動物による評価―,
第11回AOB研究会, 2011年7月. 向井理恵, 水口八重子, 藤倉温, 河野尚平, 二川健, 河合慶親, 寺尾純二 :
ケルセチンの経口摂取による廃用性筋萎縮予防の可能性,
第65回日本栄養・食糧学会大会, 2011年5月. 河村知志, 林萠未, 向井理恵, 寺尾純二, 根本尚夫 :
フラバノン類の効率的 C-8 プレニル化法,
日本薬学会年会, 2011年3月. 林萠未, 河村知志, 向井理恵, 寺尾純二, 根本尚夫 :
フラボノイドの C-プレニル化法開発と合成への応用,
日本薬学会第131年会, 2011年3月. 河村知志, 向井理恵, 林萠未, 寺尾純二, 根本尚夫 :
廃用性筋萎縮抑制作用を有するフラボノイドのC-プレニル化と活性評価,
日本薬学会年会, 2011年3月. 向井理恵, 水口八重子, 藤倉温, 河村知志, 根本尚夫, 河合慶親, 寺尾純二, 侯徳興 :
プレニル化ケルセチンの筋萎縮予防効果とその特徴,
日本農芸学会, 2011年3月. 桑田広子, 坂尾こず枝, 向井理恵, 寺尾純二, 河村知志, 根本尚夫, 藤井信, 侯徳興 :
プレニル化ケルセチンの機能性解析:炎症性因子の抑制およびその分子機構,
日本農芸学会, 2011年3月. 高坂玲亜, 栗原佳奈, 向井理恵, 寺尾純二, 山田耕路 :
尾懸垂誘導性筋萎縮に対する緑茶の抑制効果,
日本農芸化学会2011年度大会, 2011年3月. 川原千尋, 石井七瀬, 坂東紀子, 向井理恵, 河合慶親, 寺尾純二 :
ヘアレスマウス皮膚におけるリン脂質ヒドロペルオキシド誘導MMP-9タンパク発現に対する食餌性β‐カロテンの影響,
第15回日本フードファクター学会学術集会, 2010年10月. 向井理恵, 二川健, 寺尾純二 :
廃用性筋萎縮に伴う酸化ストレス上昇に対するケルセチンの効果,
第63回日本酸化ストレス学会学術集会, 2010年6月. 向井理恵, 大塚聖子, 川畑球一, 石坂朱里, 河合慶親, 寺尾純二 :
神経細胞において誘導される酸化ストレスに対するケルセチンの作用,
第64回日本栄養・食糧学会大会, 2010年5月. 向井理恵, 藤倉温, 二川健, 山本浩範, 武田英二, 寺尾純二 :
廃用性筋萎縮モデルマウスにおけるケルセチンの筋重量低下抑制効果,
日本農芸化学会2010年度大会, 2010年3月. 大塚聖子, 川畑球一, 向井理恵, 河合慶親, 寺尾純二 :
神経細胞の6-ヒドロキシドーパミン誘導酸化ストレスに対するケルセチン抱合体代謝物の抑制作用,
第14回日本フードファクター学会学術集会, 2009年11月. 大塚聖子, 川畑球一, 向井理恵, 河合慶親, 寺尾純二 :
神経細胞Neuro2Aの酸化ストレスに対するケルセチン代謝物の抑制作用,
第42回日本栄養・食糧学会中国・四国支部大会, 2009年11月. 向井理恵, 中尾玲子, 二川健, 山本浩範, 武田英二, 寺尾純二 :
マウス尾懸垂モデル系におけるケルセチンの筋萎縮抑制効果,
第14回日本フードファクター学会学術集会, 2009年11月.

研究会・報告書: 研究者総覧に該当データはありませんでした。

特許: 向井理恵 : 肥満抑制用組成物, (2019年12月), 特許第2019-230188号. 向井理恵 : プレニルナリンゲニンによる抗肥満効果, (2019年7月), 特許第2019-027号. 向井理恵 : 廃用性筋萎縮抑制剤及び廃用性筋萎縮抑制用食品組成物, (2018年8月), (2020年2月), 特許第2018-156562号 (2018年8月). 向井理恵 : 廃用性筋萎縮抑制剤及び廃用性筋萎縮抑制用食品組成物, (2018年4月), (2019年10月), 特許第2018-085635号 (2018年4月). 向井理恵 : 廃用性筋萎縮抑制剤及び廃用性筋萎縮抑制用食品組成物, (2016年2月), (2016年8月), 特許第2016-020894号. 向井理恵 : 動物の四肢固定具及び動物の四肢固定方法, (2015年3月), (2016年11月), 特許第2015-074324号. 寺尾純二, 向井理恵, 芦屋浩明, 小山寿之, 宇佐美陽子 : カテキン類の生体吸収及び蓄積改善剤, (2014年11月), (2016年5月), 特許第2014-232020号. 寺尾純二, 向井理恵, 根本尚夫, 河村知志, 福本修一 : 筋萎縮抑制剤，およびその使用方法, (2011年8月), (2013年2月), 特許第2013-035811号.
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: フラボノイドの唾液腺機能亢進効果の検証:口腔乾燥症予防に資する機能性食品の開発 (研究課題/領域番号: 24K14725 )
アミノ酸代謝に対するフラボノイドの新規機能解明：骨格筋萎縮予防と健康寿命の延伸 (研究課題/領域番号: 23K05073 )
抗筋萎縮フラボノイドによって向上する骨格筋の機能とその調節機構の解明 (研究課題/領域番号: 20K11581 )
筋萎縮からの回復を促進するポリフェノールとタンパク質との相乗効果解明 (研究課題/領域番号: 16K12721 )
廃用性筋萎縮後の筋合成を促進するプレニルフラボノイドの研究 (研究課題/領域番号: 26892020 )
機能性フラボノイドープレニル化の生理的意義の解明 (研究課題/領域番号: 25292075 )
骨格筋萎縮を予防する食事性フラボノイドの作用機構解明 (研究課題/領域番号: 23780136 )
ストレス誘導によるケルセチンの活性化と多機能発現ダイナミクスの解明 (研究課題/領域番号: 22380077 )
研究者番号（90547978）による検索

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2024年11月21日更新

専門分野・研究分野: 食品科学 (Food Science)
栄養化学 (Nutritional Chemistry)
機能性食品
所属学会・所属協会: 日本栄養食糧学会
日本フードファクター学会
日本酸化ストレス学会
社団法人日本農芸化学会
日本農芸化学会
公益社団法人日本農芸化学会中四国支部
委員歴・役員歴: 日本フードファクター学会 (参与会員 [2020年4月〜2030年3月])
社団法人日本農芸化学会 (中四国支部参与会員 [2011年4月〜2022年3月])
日本農芸化学会 (実行委員 [2020年9月])
社団法人日本農芸化学会 (広報委員 [2021年4月〜2024年])
社団法人日本農芸化学会 (中四国支部市民フォーラム世話人 [2021年4月])
社団法人日本農芸化学会 (中四国支部若手研究者シンポジウム世話人 [2022年4月〜8月])
公益社団法人日本農芸化学会中四国支部 (参与会員)
受賞: 2009年12月, Travel Award (4th International Conference on Polyphenol and Health)
2011年11月, Young Investigator Award (Internatinal Conference on Food Factors)
2015年3月, 平成26年岡奨学賞 (徳島大学)
2015年7月, 奨励賞 (AOB研究会)
2016年3月, ベストティーチャーオブザイヤー (医学部)
2016年11月, 平成28年度徳島大学若手研究者学長表彰
2017年3月, 2017年度農芸化学奨励賞
2021年1月, 2021年度ダイバーシティ推進共同研究表彰 (四国発信!ダイバーシティ研究環境調和推進プロジェクト)
2021年3月, ベストティーチャーオブザイヤー (徳島大学生物資源産業学部)
2024年度日本農芸化学会中四国支部奨励賞 (日本農芸化学会中四国支部)
活動: 徳島県立城西高等学校神山校「地域との協働による高等学校教育改革推進事業」 (運営委員 [2019年〜2022年3月])
徳島県ヘルスケアビジネス支援事業 (ヘルスケアアドバイザー [2022年6月〜2023年3月])
徳島県ヘルスケアビジネス支援事業 (ヘルスケアアドバイザー [2023年4月〜2024年3月])
FD委員会委員 (2018年4月〜2019年3月)
インターンシップ委員 (2021年4月〜2022年3月)
広報委員 (2021年4月〜2022年3月)
動物実験委員会
徳島大学大学院社会産業理工学研究部理工学域及び生物資源産業学域研究倫理委員会
入学試験委員
放射線安全管理委員会
創成科学専攻入学試験委員

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2024年11月17日更新

2024年11月16日更新

Ｊグローバル

Jグローバル最終確認日: 2024/11/16 01:28
氏名（漢字）: 向井理恵
氏名（フリガナ）: JグローバルAPIで取得できませんでした。
氏名（英字）: Mukai Rie
所属機関: 徳島大学准教授

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researchmap最終確認日: 2024/11/17 02:10
氏名（漢字）: 向井理恵
氏名（フリガナ）: リサーチマップAPIで取得できませんでした。
氏名（英字）: Mukai Rie
プロフィール: リサーチマップAPIで取得できませんでした。
登録日時: 2018/1/31 13:16
更新日時: 2024/11/16 06:34
アバター画像URI: https://researchmap.jp/riemukai/avatar.jpg
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所属ID: 0344000000
所属: 徳島大学
部署: 社会産業理工学研究部
職名: 准教授
学位: 博士（農学）
学位授与機関: 神戸大学
URL: リサーチマップAPIで取得できませんでした。
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経歴
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特許
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2024年11月16日更新

研究者番号: 90547978

所属（現在）: 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授

所属（過去の研究課題 情報に基づく）*注記: 2017/4/1 – 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授
2016/4/1 : 徳島大学, 大学院生物資源産業学研究部, 講師
2015/4/1 : 徳島大学, 大学院医歯薬学研究部, 助教
2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 助教
2011/4/1 – 2012/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 助教
2011/4/1 – 2012/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教

審査区分/研究分野

研究代表者

生物系 / 農学 / 農芸化学 / 食品科学
総合系 / 複合領域 / 生活科学 / 食生活学
小区分59040:栄養学および健康科学関連
小区分38050:食品科学関連

研究代表者以外

生物系 / 農学 / 農芸化学 / 食品科学
小区分59040:栄養学および健康科学関連

キーワード

研究代表者

フラボノイド / 筋萎縮 / 機能性食品 / 骨格筋 / ケルセチン / 筋合成 / プレニルフラボノイド / ポリフェノール / タンパク質分解 / エストロゲン / ナリンゲニン / タンパク質 / アミノ酸 / 健康 / 食品成分 / 酸化ストレス / 抗肥満 / 代謝向上 / 健康寿命

研究代表者以外

食品 / ストレス / 発現制御 / 分析化学 / シグナル伝達、筋萎縮 / β-βグルクロニダーゼ / 炎症 / シグナル伝達 / 筋萎縮 / 筋委縮 / β-グルクロニダーゼ / 分析科学 / 潰瘍性大腸炎 / フラボノイド / 食品機能 / プレニル化 / 抗酸化酵素 / 血管内皮 / 血管内皮細胞 / ヘムオキシゲナーゼー１ / カベオリン-1 / カベオラ / Nrf2 / MAPK経路 / 生体利用性 / 抱合体代謝 / 唾液腺 / 口腔乾燥症 / アクアポリン5

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2020年5月20日

食用コオロギの機能性検証と生産システムの社会実装

三戸太郎櫻谷英治宮脇克行金丸芳向井理恵杉本真弓石丸善康阪本鷹行渡辺崇人

2020年6月23日

徳島大学宇宙食品産業・栄養学研究センターによる近未来型宇宙食糧ソリューション

二川健出口祥啓河合慶親宮本賢一酒井徹濵田康弘中尾玲子向井理恵高橋章宮脇克行粟飯原睦美岸本幸治原口雅宣阪上浩竹谷豊木内陽介芥川正武榎本崇宏

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向井 理恵

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向井理恵