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伊藤博夫

徳島大学

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リサーチマップ・
Jグローバル
KAKEN
注目研究

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2024年4月19日更新

職名: 教授
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学歴: 1983/3: 大阪大学歯学部歯学科 1983年卒業
1987/3: 大阪大学大学院歯学研究科 1987修了 (歯学博士)
学位: 歯学博士 (大阪大学) (1987年3月)
職歴・経歴: 1987/4: フランス国立科学研究センター癌研究所研究員
1989/4: 大阪大学助手歯学部(口腔治療学講座)
1993/8: 九州大学助手歯学部(口腔生化学講座)
1998/4: 鹿児島大学助教授歯学部(予防歯科学講座)
2003/4: 鹿児島大学助教授大学院医歯学総合研究科発生発達成育学講座予防歯科学分野
2005/11: 徳島大学教授大学院ヘルスバイオサイエンス研究部予防歯学分野

専門分野・研究分野: 予防歯学 (Preventive Dentistry)

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2024年4月19日更新

専門分野・研究分野: 予防歯学 (Preventive Dentistry)
担当経験のある授業科目: 予防歯学 (大学院)
予防歯学演習 (大学院)
口腔保健推進予防歯学概論 (大学院)
実践口腔科学実習 (大学院)
歯周病学概論 (大学院)
社会医学・疫学・医学統計概論 (大学院)
総合歯科学三 (学部)
臨床実習 (学部)
臨床実習(5年) (学部)
顎口腔発育・社会歯科学実験実習 (大学院)
指導経験: 1人 (博士)

研究者総覧で最新データを確認する

2024年4月19日更新

専門分野・研究分野: 予防歯学 (Preventive Dentistry)

研究テーマ: • 口腔常在細菌による全身性感染症の病因と予防, • 細菌付着阻害性分子探索へのコンビナトリアルケミカルライブラリーの応用., • 歯科疾患の予防と治療に関する臨床疫学的研究., • 免疫ネットワーク/免疫学的記憶と母子免疫/粘膜免疫の関連

著書

伊藤博夫 :
口腔保健・予防歯科学第2版, --- 第2編，・第2章歯周病予防, 口腔保健・予防歯科学第1版, p. 142-147, 安井利一ほか編 ---,
医歯薬出版株式会社, 東京, 2023年3月. 伊藤博夫 :
チェアサイドQ&A予防歯科編 p.72-73, 予防歯科臨床教育協議会編著，, --- Q30歯周病検査における唾液検査の有効性は? ---,
クインテッセンス出版株式会社, 東京, 2018年12月. 伊藤博夫 :
デンタルスタッフの口腔衛生学・歯科衛生統計第1版, --- 第5章口腔疾患とその関連疾患の予防 ---,
医歯薬出版社, 2018年2月. 伊藤博夫 :
第2編，・第2章歯周病予防, 口腔保健・予防歯科学第1版, p. 142-147, 安井利一ほか編, 医歯薬出版社, 東京, 2017年2月20日.(分担),
2017年2月. 伊藤博夫 :
第4章齲蝕とその予防, 新予防歯科学第4版, p. 73-86, 米満正美ほか編, 医歯薬出版社, 東京, 2010年4月1日.(分担),
2010年4月. 伊藤博夫, 末高武彦ほか編 :
新口腔保健学第1版, --- 第5章 6. 歯周疾患とその予防 ---,
医歯薬出版社, 東京, 2009年. 吉山昌宏, 伊藤博夫, 十河基文 :
プラークコントロール法の現状と課題, MI 時代の歯科知識-プラークコントロール・サイエンス&プラクティス,
永末書店, 京都, 2009年. Kiyoshi Nokihara, Hiro-O Ito, Sakiko Soutome, Takafumi Ohyama, Shizue Yamamoto, Tadashi Yasuhara and Masakazu Inoue :
Application of Combinatorial Peptide Libraries to Prevention of Infectious Endocarditis, as an Example for Infection Control,
The Japanese Peptide Society, Osaka, Mar. 2003. Y Okada, Hiro-O Ito, K Kitada and M Inoue :
Infectivity of nutritionally variant streptococci: capacity of endocarditis induction and extracellular matrix binding. p.653-656, In : Martin, D. R. and Tagg, J. R. (Eds.) Streptococci and streptococcal diseases.,
Proceedings of the XIV Lancefield International, Priura, New Zealand, 2000.

論文

Michiyasu Nakao, Akihito Nakamura, Tomoyuki Takesue, Syuji Kitaike, Hiro-O Ito and Shigeki Sano :
Synthesis of Novel Phosphorus-Substituted Stable Isoindoles by a Three-Component Coupling Reaction of ortho-Phthalaldehyde, 9,10-Dihydro-9-oxa-10-phosphaphenanthrene 10-Oxide, and Primary Amines,
SynOpen, Vol.7, No.3, 394-400, 2023.

(要約): A three-component coupling reaction of ortho-phthalaldehyde, 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide, and various primary amines readily afforded novel phosphorus-substituted stable isoindoles in good to excellent yields. The importance of the reversible ring-opening of 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide by methanolysis in the three-component coupling reaction became apparent.
(キーワード): OPA method / isoindoles / ortho-phthalaldehyde / 9,10-dihydro-9- oxa-10-phosphaphenanthrene 10-oxide / primary amines / isoindolin-1-ones / ring-opening / methanolysis
(徳島大学機関リポジトリ): ● Metadata: 118534
(出版サイトへのリンク): ● Publication site (DOI): 10.1055/a-2148-9433
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85170846368

(徳島大学機関リポジトリ: 118534, DOI: 10.1055/a-2148-9433, Elsevier: Scopus) Akari Watanabe, Naofumi Tamaki, Kenji Yokota, Susumu Kokeguchi, Hiro-O Ito and Miwa Matsuyama :
Use of ATP Bioluminescence Assay to Evaluate Oral Streptococci,
Biocontrol Science, Vol.27, No.4, 229-233, 2022.

(要約): The statistical correlation between the number of oral streptococci and the results of ATP bioluminescence assay was examined and compared with the results from Streptococcus plate counts and an oral bacteria quantification system. Because a significant correlation was found between ATP (RLU) and the number of bacteria in the oral bacteria quantification system for all seven types of oral streptococci examined, ATP would reflect a conditions of oral hygiene. However, using this assay, it was observed it may be difficult to correctly evaluate bacteria that form aggregates. Furthermore, even a small number of bacteria (below 10 CFU/mL) , which cannot be measured by the oral bacteria quantification system, could be estimated by using ATP bioluminescence assay. It was suggested that this assay could be used for quantitative evaluation of the effect of oral cleaning.
(キーワード): Adenosine Triphosphate / Bacteria / Streptococcus / Luminescent Measurements / Colony Count, Microbial
(出版サイトへのリンク): ● Publication site (DOI): 10.4265/bio.27.229
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36567120; ● Search Scopus @ Elsevier (PMID): 36567120; ● Search Scopus @ Elsevier (DOI): 10.4265/bio.27.229

(DOI: 10.4265/bio.27.229, PubMed: 36567120) Shu Chen, Naofumi Tamaki, Yasusei Kudo, Takaaki Tsunematsu, Kaname Miki, Naozumi Ishimaru and Hiro-O Ito :
Protective effects of resveratrol against 5-fluorouracil-induced oxidative stress and inflammatory responses in human keratinocytes.,
Journal of Clinical Biochemistry and Nutrition, Vol.69, No.3, 238-246, 2021.

(要約): -acetylcysteine reduced ROS levels without affecting the expression of pro-inflammatory cytokines. Resveratrol might be useful for preventing 5-FU-induced adverse effects by activating anti-oxidant and anti-inflammatory responses.
(徳島大学機関リポジトリ): ● Metadata: 116380
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.21-23
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34857985; ● Search Scopus @ Elsevier (PMID): 34857985; ● Search Scopus @ Elsevier (DOI): 10.3164/jcbn.21-23

(徳島大学機関リポジトリ: 116380, DOI: 10.3164/jcbn.21-23, PubMed: 34857985) Masaki GOTO, Akira Kazama, Kensuke Fukuhara, Honami Sato, Nobutake Tamai, Hiro-O Ito and Hitoshi Matsuki :
Membrane fusion of phospholipid bilayers under high pressure: Spherical and irreversible growth of giant vesicles,
Biophysical Chemistry, Vol.277, 106639-1-106639-11, 2021.

(要約): Membrane fusion of giant vesicles (GVs) for binary bilayers of unsaturated phospholipids, dioleoylphosphatidylethanolamine (DOPE) having an ability to promote membrane fusion and its homolog dioleoylphosphatidylcholine (DOPC) having an ability to form GV, was investigated under atmospheric and high pressure. While DOPC formed GVs in the presence of inorganic salts with a multivalent metal ion under atmospheric pressure, an equimolar mixture of DOPE and DOPC formed GVs both in the absence and the presence of LaCl3. We examined the change in size and shape of the GVs of this binary mixture in the absence and presence of LaCl3 as a function of time under atmospheric and high pressure. The size and shape of the GVs in the absence of LaCl3 under atmospheric and high pressure and those in the presence of LaCl3 under atmospheric pressure hardly changed with time. By contrast, the GV in the presence of LaCl3 under high pressure gradually changed in the size and shape with time on a time scale of several hours. Namely, the GV became larger than the original GV due to accelerated membrane fusion and its shape became more spherical. This pressure-induced membrane fusion was completely irreversible, and the growth rate was correlated with the applied pressure. The reason for the GV growth by applying pressure was considered on the basis of thermodynamic phase diagrams. We concluded that the growth is attributable to a closer packing of lipid molecules in the bilayer resulting from their preference of smaller volumes under high pressure. Furthermore, the molecular mechanism of the pressure-induced membrane fusion was explored by observing the fusion of two GVs with almost the same size. From their morphological changes, we revealed that the fusion is caused by the actions of Laplace and osmotic pressure.
(キーワード): Giant vesicle / 高圧力 (high pressure) / Laplace pressure / Lipid bilayer membrane / Osmotic pressure / Membrane fusion
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bpc.2021.106639
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2021.106639

(DOI: 10.1016/j.bpc.2021.106639) A Y Usui K Hirata Tominaga, Hiro-O Ito and K Nokihara :
Applications of a novel biodetection system to saliva using protein fingerprints with data processing.,
Bioorganic & Medicinal Chemistry, Vol.26, 3210-3216, 2018.

(要約): A fundamental method has been developed focusing on a facile and rapid examination of periodontal disease. Periodontal disease is an oral disease thought to affect 80% of adults, and early detection with treatment is desirable for the improvement of the quality of life. Unfortunately conventional methods are not consistent as the disease is caused by a number of undefined bacteria and detection relies on the skills of the dentist. Thus an objective detection system is required. We have performed an experiment on saliva using a novel biodetection system, designated PepTenChip®. A disease model for saliva was prepared using a specimen from a healthy subject and a mixture of hemoglobin (f-Hb) and lactate dehydrogenase (LDH), which is used as a periodontal disease marker protein with healthy saliva. PepTenChip® is a peptide microarray in which fluorescent labelled structured peptides are immobilized on a novel amorphous carbon substrate. Since the peptides used as capture molecules are fluorescently labelled, labeling of analytes is not necessary. The fluorescence intensity change before and after application of analytes are detected rather than the ON/OFF detection common to conventional microarrays using a set of antigen-antibody. The fluorescence intensity value changes according to the concentration of captured protein allowing the generation of protein fingerprint (PFP) and dendrograms. The present method does not rely on a "one to one" interaction, unlike conventional biodetection, and advantages can be envisaged in the case of an undefined or unknown cause of disease. The statistical analyses, such as multivariate analyses, allow classification of the type of proteins added in saliva as mimetics of disease. PepTenChip® system is useful and convenient for examination of periodontal disease in health care.
(キーワード): Amino Acid Sequence / Analysis of Variance / Biomarkers / Fetal Hemoglobin / Fluorescent Dyes / Humans / L-Lactate Dehydrogenase / Peptide Library / Peptides / Periodontal Diseases / Principal Component Analysis / Protein Array Analysis / Saliva
(徳島大学機関リポジトリ): ● Metadata: 113943
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bmc.2018.04.049
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29706528; ● Summary page in Scopus @ Elsevier: 2-s2.0-85046132609

(徳島大学機関リポジトリ: 113943, DOI: 10.1016/j.bmc.2018.04.049, PubMed: 29706528, Elsevier: Scopus) Michiyasu Nakao, Nanako Nishikiori, Akihito Nakamura, Murasaki Miyagi, Nao Shibata, Syuji Kitaike, Makoto Fukui, Hiro-O Ito and Shigeki Sano :
Synthesis of Sterically Protected Isoindoles from ortho-Phthalaldehyde,
SynOpen, Vol.2, No.1, 50-57, 2018.

(要約): o-Phthalaldehyde (OPA) reacts with O-protected tris(hydroxyalkyl)aminomethanes in the presence of 1-propanethiol to afford a novel class of stable isoindoles. Steric protection provided by the bulkiness of C 3-symmetric primary amines derived from tris(hydroxymethyl)aminomethane could be significant for the stabilization of 1-alkylthio-2-alkyl-substituted isoindoles derived from OPA. A plausible reaction mechanism is proposed to explain the formation of the isoindole and an isoindolin-1-one by-product.
(キーワード): o-phthalaldehyde / isoindole / steric protection / isoindolin-1-one
(徳島大学機関リポジトリ): ● Metadata: 113941
(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-0036-1591932
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1055/s-0036-1591932

(徳島大学機関リポジトリ: 113941, DOI: 10.1055/s-0036-1591932) 玉木直文, 松尾亮, 水野昭彦, 正木文浩, 中川徹, 鈴木雅博, 福井誠, 谷口隆司, 三宅達郎, 伊藤博夫 :
歯肉溝液中の炎症関連バイオマーカーと糖尿病・腎機能マーカーとの関連性,
口腔衛生学会雑誌, Vol.66, No.3, 316-321, 2016年.

(出版サイトへのリンク): ● Publication site (DOI): 10.5834/jdh.66.3_316
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205002850304; ● Search Scopus @ Elsevier (DOI): 10.5834/jdh.66.3_316

(DOI: 10.5834/jdh.66.3_316, CiNii: 1390001205002850304) Naofumi Tamaki, Rita Cristina Orihuela Campos, Makoto Fukui and Hiro-O Ito :
Hydrogen-Rich Water Intake Accelerates Oral Palatal Wound Healing via Activation of the Nrf2/Antioxidant Defense Pathways in a Rat Model.,
Oxidative Medicine and Cellular Longevity, Vol.2016, 5679040, 2016.

(要約): The wound healing process attempts to restore the integrity and function of the injured tissue. Additionally, proinflammatory cytokines, growth factors, and oxidative stress play important roles in wound healing. The aim of this study was to determine whether hydrogen-rich water intake induces the activation of the Nrf2/antioxidant defense pathway in rat palatal tissue, thereby reducing systemic oxidative stress and proinflammatory cytokine levels and promoting healing-associated genes. A circular excisional wound was created in the oral palatal region, and the wound healing process was observed. The rats were divided into two experimental groups in which either hydrogen-rich water or distilled water was consumed. In the drinking hydrogen-rich water, the palatal wound healing process was accelerated compared to that in the control group. As molecular hydrogen upregulated the Nrf2 pathway, systemic oxidative stresses were decreased by the activation of antioxidant activity. Furthermore, hydrogen-rich water intake reduced proinflammatory cytokine levels and promoted the expression of healing-associated factors in rat palatal tissue. In conclusion, hydrogen-rich water intake exhibited multiple beneficial effects through activation of the Nrf2/antioxidant defense pathway. The results of this study support the hypothesis that oral administration of hydrogen-rich water benefits the wound healing process by decreasing oxidative stress and inflammatory responses.
(キーワード): Animals / Antioxidants / Biomarkers / Chemokines / Disease Models, Animal / Drinking Behavior / Gene Expression Regulation / Hydrogen / Inflammation Mediators / Male / NF-E2-Related Factor 2 / Nitric Oxide Synthase Type II / Oxidative Stress / Palate / Rats, Wistar / Real-Time Polymerase Chain Reaction / Signal Transduction / Water / Wound Healing
(徳島大学機関リポジトリ): ● Metadata: 109746
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2016/5679040
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26798423; ● Search Scopus @ Elsevier (PMID): 26798423; ● Search Scopus @ Elsevier (DOI): 10.1155/2016/5679040

(徳島大学機関リポジトリ: 109746, DOI: 10.1155/2016/5679040, PubMed: 26798423) S Hayashi, Hirotsugu Yamada, Makoto Fukui, Hiro-O Ito and Masataka Sata :
Correlation between arteriosclerosis and periodontal condition assessed by lactoferrin and [alpha]1-antitrypsin levels in gingival crevicular fluid.,
International Heart Journal, Vol.56, No.6, 639-643, 2015.

(要約): Patients with periodontal disease exhibit exacerbated atherosclerosis, aortic stiffness, or vascular endothelial dysfunction. However, in a recent scientific statement, the American Heart Association noted that neither has periodontal disease been proven to cause atherosclerotic vascular disease nor has the treatment of periodontal disease been proven to prevent atherosclerotic vascular disease. Therefore, the aim of the present study was to examine the correlation between periodontal condition and arteriosclerosis in patients with coronary artery disease (CAD), which is usually accompanied by systemic arteriosclerosis.We measured levels of gingival crevicular fluid lactoferrin (GCF-Lf) and 1-antitrypsin (GCF-AT) in 72 patients (67 ± 8 years, 56 men) with CAD. Furthermore, we evaluated the maximum intima-media thickness (max IMT) and plaque score of the carotid arteries as well as brachial-ankle pulse wave velocity (baPWV) and flow-mediated dilation (FMD) of the brachial artery, each of which is a parameter for determining arteriosclerosis status. The average level of GCF-Lf was 0.29 ± 0.36 µg/mL and that of GCF-AT was 0.31 ± 0.66 µg/mL, with significant correlation between the two (r = 0.701, P < 0.001). No significant difference in GCF-Lf and GCF-AT levels was observed between patients with single-, double-, and triple-vessel CAD. There were no significant correlations between the arteriosclerosis parameters (ie, max IMT, plaque score, baPWV, and FMD) and GCF-Lf or GCF-AT.No correlation between the GCF biomarkers and the severity of arteriosclerosis was detected. This result may suggest that worsening of the periodontal condition assessed by GCF biomarkers is not a major potential risk factor for arteriosclerosis.
(キーワード): Periodontal disease / Coronary artery disease / Atherosclerosis
(出版サイトへのリンク): ● Publication site (DOI): 10.1536/ihj.15-218
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26549390; ● Search Scopus @ Elsevier (PMID): 26549390; ● Search Scopus @ Elsevier (DOI): 10.1536/ihj.15-218

(DOI: 10.1536/ihj.15-218, PubMed: 26549390) Naofumi Tamaki, Fumihiko Yoshino, Makoto Fukui, Hideaki Hayashida, Ayaka Yoshida, Masayasu Kitamura, Takahiro Iwasaki, Reiko Furugen, Koji Kawasaki, Mio Nakazato, Takahiro Maeda, Susumu Kokeguchi, Tatsuo Yamamoto, Chang-Il Masaichi Lee, Hiro-O Ito and Toshiyuki Saito :
Relationship among salivary antioxidant activity, cytokines and periodontitis: the Nagasaki Island study.,
Journal of Clinical Periodontology, Vol.42, No.8, 711-718, 2015.

(要約): These findings suggest that the evaluation of salivary antioxidant activities, as assessed by electron spin resonance, are associated with periodontitis and various clinical variables in community-dwelling participants (ClinicalTrials. gov number NCT01742728). This article is protected by copyright. All rights reserved.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jcpe.12438
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26227009; ● Summary page in Scopus @ Elsevier: 2-s2.0-84941261275

(DOI: 10.1111/jcpe.12438, PubMed: 26227009, Elsevier: Scopus) Rita Cristina Orihuela Campos, Naofumi Tamaki, Rie Mukai, Makoto Fukui, Kaname Miki, Junji Terao and Hiro-O Ito :
Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts.,
Journal of Clinical Biochemistry and Nutrition, Vol.56, No.3, 220-227, 2015.

(要約): In periodontitis, production of reactive oxygen species (ROS) by neutrophils induces oxidative stress and deteriorates surrounding tissues. Antioxidants reduce damage caused by ROS and are used to treat diseases involving oxidative stress. This study summarizes the different effects of resveratrol, quercetin, and N-acetylcysteine (NAC) on human gingival fibroblasts (HGFs) under oxidative stress induced by hydrogen peroxide. Real-time cytotoxicity analyses reveals that resveratrol and quercetin enhanced cell proliferation even under oxidative stress. Of the antioxidants tested, resveratrol is the most effective at inhibiting ROS production. HGFs incubated with resveratrol and quercetin up-regulate the transcription of type I collagen gene after 3 h, but only resveratrol sustained this up-regulation for 24 h. A measurement of the oxygen consumption rate (OCR, mitochondrial respiration) shows that resveratrol generates the highest maximal respiratory capacity, followed by quercetin and NAC. Simultaneous measurement of OCR and the extracellular acidification rate (non-mitochondrial respiration) reveals that resveratrol and quercetin induce an increase in mitochondrial respiration when compared with untreated cells. NAC treatment consumes less oxygen and enhances more non-mitochondrial respiration. In conclusion, resveratrol is the most effective antioxidant in terms of real-time cytotoxicity analysis, reduction of ROS production, and enhancement of type I collagen synthesis and mitochondrial respiration in HGFs.
(徳島大学機関リポジトリ): ● Metadata: 109399
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.14-129
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26060353; ● Summary page in Scopus @ Elsevier: 2-s2.0-84929618900

(徳島大学機関リポジトリ: 109399, DOI: 10.3164/jcbn.14-129, PubMed: 26060353, Elsevier: Scopus) Naofumi Tamaki, Rita Cristina Orihuela Campos, Yuji Inagaki, Makoto Fukui, Toshihiko Nagata and Hiro-O Ito :
Resveratrol improves oxidative stress and prevents the progression of periodontitis via the activation of the Sirt1/AMPK and the Nrf2/antioxidant defense pathways in a rat periodontitis model.,
Free Radical Biology and Medicine, Vol.75, 222-229, 2014.

(要約): Oxidative stress is a key factor regulating the systemic pathophysiological effects associated with periodontitis. Resveratrol is a phytochemical with antioxidant and anti-inflammatory properties that can reduce oxidative stress and inflammation. We hypothesized that resveratrol may prevent the progression of periodontitis and reduce systemic oxidative stress through the activation of the Sirtuin1 (Sirt1)/AMP-activated protein kinase (AMPK) and the nuclear factor-E2 related factor 2 (Nrf2)/antioxidant defense pathways. Three groups of male Wistar rats (periodontitis treated with melinjo resveratrol, periodontitis without resveratrol, and control rats with no periodontitis or resveratrol treatment) were examined. A ligature was placed around the maxillary molars for 3 weeks to induce periodontitis, and the rats were then given drinking water with or without melinjo resveratrol. In rats with periodontitis, ligature placement induced alveolar bone resorption, quantified using three-dimensional images taken by micro-CT, and increased proinflammatory cytokine levels in gingival tissue. Melinjo resveratrol intake relieved alveolar bone resorption and activated the Sirt1/AMPK and the Nrf2/antioxidant defense pathways in inflamed gingival tissues. Further, melinjo resveratrol improved the systemic levels of 8-hydroxydeoxyguanosine, dityrosine, nitric oxide metabolism, nitrotyrosine, and proinflammatory cytokines. We concluded that oral administration of melinjo resveratrol may prevent the progression of ligature-induced periodontitis and improve systemic oxidative and nitrosative stress.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.freeradbiomed.2014.07.034
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25091897; ● Search Scopus @ Elsevier (PMID): 25091897; ● Search Scopus @ Elsevier (DOI): 10.1016/j.freeradbiomed.2014.07.034

(DOI: 10.1016/j.freeradbiomed.2014.07.034, PubMed: 25091897) Naofumi Tamaki, H Hayashida, Makoto Fukui, M Kitamura, K Kawasaki, M Nakazato, T Maeda, T Saito and Hiro-O Ito :
Oxidative stress and antibody levels to periodontal bacteria in adults: the Nagasaki Islands study.,
Oral Diseases, Vol.20, No.3, 49-56, 2014.

(要約): OBJECTIVE: Periodontitis is a chronic inflammatory disease of the tissues supporting the teeth and is caused by subgingival plaque. Systemic increases in reactive oxygen species are involved in pathogenesis of periodontitis. This study addressed the relationship between levels of serum oxidative stress and antibodies against putative periodontopathic bacteria and their association with periodontal conditions, in a community-based study. SUBJECTS AND METHODS: Serum samples were measured for reactive oxygen metabolite (ROM) levels and anti-oxidant capacity. The serum levels of immunoglobulin G (IgG) antibodies to Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), and Eikenella corrodens (Ec) were determined by ELISA. RESULTS: The participants with greater clinical attachment loss had higher serum ROM levels and IgG antibody titers to Pg. Serum ROM levels were positively correlated with antibody titers to Pg, Pi, and Ec. When the participants with greater probing pocket depth and clinical attachment loss were used as the dependent variables, high ROM levels showed a statistically significant associations in multivariate logistic analyses; the adjusted odds ratios were 2.9 (95% confidence interval = 1.0-8.5) and 6.0 (95% confidence interval = 2.0-17.6), respectively. CONCLUSIONS: It was concluded that an increased oxidative stress may be detrimental to periodontitis in Japanese community-dwelling adults.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/odi.12127
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23679350; ● Summary page in Scopus @ Elsevier: 2-s2.0-84896099990

(DOI: 10.1111/odi.12127, PubMed: 23679350, Elsevier: Scopus) Yoshikazu Fukumoto, Masumi Horibe, Yuji Inagaki, Keiji Oishi, Naofumi Tamaki, Hiro-O Ito and Toshihiko Nagata :
Association of gingival recession and other factors with the presence of dentin hypersensitivity.,
Odontology, 2013.

(要約): Dentin hypersensitivity (DH) may be present in association with gingival recession. The aim of this study was to determine quantitatively the association of gingival recession and other factors with the presence of DH. One hundred and four Japanese subjects with or without gingival recession were randomly selected. Intact canines and/or first premolars in both maxillary and mandibular quadrants were analyzed. Gingival recession was measured as a vertical length at the buccal site of the teeth. DH was recorded as an ordered categorical variable registering four increasing levels of pain after cold stimulation; from no discomfort to severe pain during and after stimulation (DH1, 2, 3, and 4). Association of DH with periodontal parameters and daily lifestyle was also investigated. Tooth-based analysis of 446 teeth from 104 subjects revealed that DH level was significantly higher in recessive teeth (1, 2, 3, and 4-8 mm) than in non-recessive teeth (0 mm). DH-positive rate in non-recessive teeth was only 18 % (DH1; 14 %, DH2; 3 %, and DH3; 1 %). Highest DH level was observed in teeth with severe recession (4-8 mm), showing DH0; 21 %, DH1; 33 %, DH2; 31 %, and DH3; 15 %. Recession-dependent increase in DH was observed, showing 18, 49, 52, 60, and 79 % DH-positive in teeth with 0, 1, 2, 3, and 4-8 mm recession, respectively. Plaque-free teeth showed a higher DH level than plaque-stained teeth, suggesting that good plaque control may be associated with the presence of DH. There were no significant differences in DH of teeth on the basis of smoking, probing depth, and bleeding on probing. Multiple logistic regression analysis revealed that gingival recession [odds ratio (OR) = 10.2, 95 % confidence interval (CI) = 5.5-18.9] and plaque deposition (OR = 0.3, 95 % CI = 0.2-0.5) were significant contributors to DH. Multilevel modeling analysis revealed that not only gingival recession and plaque deposition but also V-shaped cervical notch and tooth brushing frequency were associated with DH. These results demonstrate that the progression of gingival recession, plaque-free teeth, V-shaped cervical notch, and frequent brushing may be significant predictors of DH in canines and first premolars.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10266-012-0099-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23283584; ● Search Scopus @ Elsevier (PMID): 23283584; ● Search Scopus @ Elsevier (DOI): 10.1007/s10266-012-0099-5

(DOI: 10.1007/s10266-012-0099-5, PubMed: 23283584) Masaki GOTO, Agnieszka Wilk, Kosuke Kataoka, Shirish Chodankar, Nobutake Tamai, Makoto Fukui, Joachim Kohlbrecher, Hiro-O Ito and Hitoshi Matsuki :
Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry,
Langmuir, Vol.28, No.33, 12191-12198, 2012.

(要約): The myristoylpalmitoylphosphatidylcholine (MPPC) bilayer membrane shows a complicated temperature-pressure phase diagram. The large portion of the lamellar gel (L(β)'), ripple gel (P(β)'), and pressure-induced gel (L(β)I) phases exist as metastable phases due to the extremely stable subgel (L(c)) phase. The stable L(c) phase enables us to examine the properties of the L(c) phase. The phases of the MPPC bilayers under atmospheric and high pressures were studied by small-angle neutron scattering (SANS) and fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan. The SANS measurements clearly demonstrated the existence of the metastable L(β)I phase with the smallest lamellar repeat distance. From a second-derivative analysis of the fluorescence data, the line shape for the L(c) phase under high pressure was characterized by a broad peak with a minimum of ca. 460 nm. The line shapes and the minimum intensity wavelength (λ''(min)) values changed with pressure, indicating that the L(c) phase has highly pressure-sensible structure. The λ''(min) values of the L(c) phase spectra were split into ca. 430 and 500 nm in the L(β)I phase region, which corresponds to the formation of a interdigitated subgel L(c) (L(c)I) phase. Moreover, the phase transitions related to the L(c) phase were reversible transitions under high pressure. Taking into account the fluorescence behavior of Prodan for the L(c) phase, we concluded that the structure of the L(c) phase is highly probably a staggered structure, which can transform into the L(c)I phase easily.
(キーワード): 2-Naphthylamine / Atmospheric Pressure / Cell Membrane / Fluorometry / Gels / Lipid Bilayers / Neutron Diffraction / Phosphatidylcholines / Pressure / Scattering, Small Angle
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/la3020173
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22823885; ● Summary page in Scopus @ Elsevier: 2-s2.0-84865495527

(DOI: 10.1021/la3020173, PubMed: 22823885, Elsevier: Scopus) Tselmeg Baatarjav, Kosuke Kataoka, Rebekah S. Gilbert, Yutaka Terao, Makoto Fukui, Masaki GOTO, Shigetada Kawabata, Masato Yamamoto, Kohtaro Fujihashi and Hiro-O Ito :
Mucosal immune features to phosphorylcholine by nasal Flt3 ligand cDNA-based vaccination,
Vaccine, Vol.29, No.34, 5747-5757, 2011.

(要約): Phosphorylcholine (PC) is an immunodominant epitope in some pathogens including Streptococcus pneumoniae and it is well-known that PC-specific antibodies (Abs) play a key role in the induction of protective immunity against pneumococcal infection. In this study, we examined whether nasal administration of DNA plasmid encoding Flt3 ligand gene (pFL) as a mucosal adjuvant plus PC-conjugated keyhole limpet hemocyanin (PC-KLH), would elicit PC-specific immune responses, and characterized mucosal immune responses to PC induced by this nasal vaccination. Nasal immunization with pFL plus PC-KLH enhanced induction of PC-specific IgA and IgM Abs in airway secretions when compared with mice given PC-KLH with or without empty plasmid gene (pORF) as controls; in addition to the mucosal immune responses, PC-specific immune responses in serum were also induced. Furthermore, the mucosal and serum IgA and IgM Abs in mice given pFL plus PC-KLH nasally, exhibited high-specificity for the PC molecule. Of interest, the PC-specific Abs bound dose-dependently to anti-T15 idiotype (AB1-2). Thus, the inhibition of S. pneumoniae colonization on the nasal cavity and lungs after nasal challenge with the live organism was significantly elicited in mice immunized with pFL plus PC-KLH compared to that of mice immunized with antigen with pORF. Taken together, these findings show that nasal administration of pFL with PC-KLH elicited T15-like anti-PC IgA and IgM Abs in the respiratory tracts, and further attenuated S. pneumoniae colonization on the respiratory tracts. Nasal administration of Flt3 ligand cDNA with PC may contribute to the development of nasal vaccination for prevention of S. pneumoniae infection.
(キーワード): Administration, Intranasal / Administration, Mucosal / Animals / Antibodies, Bacterial / Bacterial Vaccines / DNA, Complementary / Hemocyanin / Immunity, Mucosal / Immunoglobulin A / Immunoglobulin M / Membrane Proteins / Mice / Mice, Inbred C57BL / Phosphorylcholine / Plasmids / Pneumococcal Infections / Streptococcus pneumoniae / Vaccines, DNA
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.vaccine.2011.05.097
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21683111; ● Summary page in Scopus @ Elsevier: 2-s2.0-79960560899

(DOI: 10.1016/j.vaccine.2011.05.097, PubMed: 21683111, Elsevier: Scopus) Kosuke Kataoka, Kohtaro Fujihashi, Keita Oma, Yoshiko Fukuyama, K Susan Hollingshead, Shinichi Sekine, Shigetada Kawabata, Hiro-O Ito, E David Briles and Kazunori Oishi :
Nasal Dendritic Cell Targeting Flt3 Ligand As A Safe Adjuvant Elicits Effective Protection Against Fatal Pneumococcal Pneumonia.,
Infection and Immunity, Vol.79, No.7, 2819-2828, 2011.

(要約): We have previously shown that pneumococcal surface protein A (PspA)-based vaccine containing DNA plasmid encoding the Flt3 ligand (FL) gene (pFL) as nasal adjuvant prevented nasal carriage of Streptococcus pneumoniae. In this study, we further investigated the safety and efficacy of this nasal vaccine for the induction of PspA-specific antibody (Ab) responses against lung infection with S. pneumoniae. C57BL/6 mice were nasally immunized with recombinant PspA/Rx1 (rPspA) plus pFL three times at weekly intervals. When dynamic translocation of pFL was initially examined, nasal pFL was taken-up by nasal dendritic cells (DCs) and epithelial cells (nECs), but not in the central nervous systems including olfactory nerve and epithelium. Of importance, nasal pFL induced FL protein synthesis with minimum levels of inflammatory cytokines in the nasal washes (NWs) and bronchoalveolar lavage fluid (BALF). NWs and BALF as well as plasma of mice given nasal rPspA plus pFL contained increased levels of rPspA-specific secretory-IgA and IgG Ab responses that are correlated with elevated numbers of CD8(+) and CD11b(+) DCs and IL-2 and IL-4 producing CD4(+) T cells in the nasopharyngeal-associated lymphoid tisses (NALT) and cervical lymph nodes (CLNs). The in vivo protection by rPspA-specific Abs was evident by reduced numbers of CFU in the lungs, airway secretions, and blood when mice were nasally challenged with Streptococcus pneumoniae WU2. Our findings show that nasal pFL is a safe and effective mucosal adjuvant for the enhancement of bacterial Ag (rPspA)-specific protective immunity through DC-induced Th2-type and IL-2 cytokine responses.
(キーワード): Adjuvants, Immunologic / Administration, Intranasal / Animals / Antibodies, Bacterial / Bacterial Proteins / Bronchoalveolar Lavage Fluid / CD4-Positive T-Lymphocytes / CD8-Positive T-Lymphocytes / 細胞質分裂 (cytokinesis) / Dendritic Cells / Epithelial Cells / Immunoglobulin A, Secretory / Immunoglobulin G / Membrane Proteins / Mice / Mice, Inbred C57BL / Nasal Cavity / Nasal Mucosa / Nasal Sprays / Plasmids / Pneumococcal Vaccines / Pneumonia, Pneumococcal / Recombinant Proteins / Streptococcus pneumoniae / Vaccines, Synthetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.01360-10
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21536790; ● Search Scopus @ Elsevier (PMID): 21536790; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.01360-10

(DOI: 10.1128/IAI.01360-10, PubMed: 21536790) Makoto Fukui, Daisuke Hinode, Masaaki Yokoyama, Masami Yoshioka, Kosuke Kataoka and Hiro-O Ito :
Levels of salivary stress markers in patients with anxiety about halitosis,
Archives of Oral Biology, Vol.55, No.11, 842-847, 2010.

(要約): To investigate the relationship between salivary stress markers and mental stress states in patients complaining of oral malodour. The utility of the salivary stress markers in assessment of mental conditions of those patients was also investigated. The study population included 74 patients, aged 20-59 years, who complained of oral malodour and were referred to the Breath Odor Clinic at Tokushima University Hospital. Patients were classified into two groups, genuine halitosis (GH) and psychosomatic halitosis (PH), according to the results of organoleptic rating measurement. All patients were subjected to examination by the Cornell Medical Index (CMI) Health Questionnaire. Resting saliva was collected and levels of salivary IgA, cortisol and chromogranin A were determined by ELISA. Twenty-three volunteers not complaining of halitosis were included as the control group. Kruskal-Wallis test and Mann-Whitney's U-test were used for statistical analysis. A significant increase was observed in the concentrations of salivary cortisol in the PH group as compared with GH and control groups (p<0.05). Concentrations of IgA and chromogranin A in saliva were not significantly different among the three groups. In addition, higher salivary cortisol concentrations were found in CMI scale III and IV (tendency towards neurosis) than in scale I and II (normal) (p<0.05). Since salivary cortisol reflects a status of chronic stress condition, psychosomatic halitosis might be closely related to this state of chronic stress. Determination of cortisol levels in saliva may provide useful information for evaluating the mental status of patients complaining of halitosis.
(キーワード): Adult / Anxiety / Biological Markers / Case-Control Studies / Chromogranin A / Enzyme-Linked Immunosorbent Assay / Female / Halitosis / Humans / Hydrocortisone / Immunoglobulin A / Male / Middle Aged / Psychophysiologic Disorders / Questionnaires / Saliva / Statistics, Nonparametric
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.archoralbio.2010.07.014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20807658; ● Search Scopus @ Elsevier (PMID): 20807658; ● Search Scopus @ Elsevier (DOI): 10.1016/j.archoralbio.2010.07.014

(DOI: 10.1016/j.archoralbio.2010.07.014, PubMed: 20807658) Etsuhisa Takahashi, Kosuke Kataoka, Kazuyuki Fujii, Junji Chida, Dai Mizuno, Makoto Fukui, Hiro-O Ito, Kohtaro Fujihashi and Hiroshi Kido :
Attenuation of inducible respiratory immune responses by oseltamivir treatment in mice infected with influenza A virus.,
Microbes and Infection, Vol.12, No.10, 778-783, 2010.

(要約): The antiviral neuraminidase inhibitor oseltamivir (OSV) is widely used to suppress viral replication in the treatment of influenza. Here, we report that OSV administration significantly suppressed respiratory mucosal secretory IgA responses with respect to antigen (Ag)-specific antibody (Ab) production and also the induction of Ag-specific IgA Ab-forming cells, but not systemic IgG responses, in weanling mice as a model of pediatric influenza. Neutralizing activities of the airway fluids in oral OSV-treated mice were significantly less than those of sham-treated mice. Our findings suggest the risk of re-infection in patients showing a low mucosal response following OSV treatment.
(キーワード): Animals / Antibodies, Viral / Antiviral Agents / Female / Immunity, Mucosal / Immunoglobulin A / Immunoglobulin G / Immunosuppression / Immunosuppressive Agents / Influenza A virus / Mice / Mice, Inbred BALB C / Orthomyxoviridae Infections / Oseltamivir
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.micinf.2010.04.013
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20452454; ● Search Scopus @ Elsevier (PMID): 20452454; ● Search Scopus @ Elsevier (DOI): 10.1016/j.micinf.2010.04.013

(DOI: 10.1016/j.micinf.2010.04.013, PubMed: 20452454) Hidetaka Nakagaki, Shinichi Sekine, Yutaka Terao, Masahiro Toe, Muneo Tanaka, Hiro-O Ito, Shigetada Kawabata, Satoshi Shizukuishi, Kohtaro Fujihashi and Kosuke Kataoka :
Fusobacterium nucleatum envelope protein FomA is immunogenic and binds to the salivary statherin-derived peptide.,
Infection and Immunity, Vol.78, No.3, 1185-1192, 2010.

(要約): We have previously shown that one of the minimal active regions of statherin, a human salivary protein, for binding to Fusobacterium nucleatum is a YQPVPE amino acid sequence. In this study, we identified the FomA protein of F. nucleatum, which is responsible for binding to the statherin-derived YQPVPE peptide. Overlay analysis showed that a 40-kDa protein of the F. nucleatum cell envelope (40-kDa CE) specifically bound to the YQPVPE peptide. The equilibrium association constant between the affinity-purified 40-kDa CE and the YQPVPE peptide was 4.30 x 10(6). Further, the purity and amino acid sequence analyses of the purified 40-kDa CE revealed approximately 98.7% (wt/wt) purity and a high degree of homology with FomA, a major porin protein of F. nucleatum. Thus, a FomA-deficient mutant failed to bind to the YQPVPE peptide. In addition, increased levels of a FomA-specific mucosal IgA antibody (Ab) and plasma IgG and IgA Abs were seen only in mice immunized nasally with cholera toxin (CT) and the purified 40-kDa FomA protein. Interestingly, saliva from mice that received FomA plus CT as a mucosal adjuvant nasally prevented in vitro binding of F. nucleatum to statherin-coated polyvinyl chloride plates. Taken together, these results suggest that induction of specific immunity to the 40-kDa FomA protein of F. nucleatum, which specifically binds to the statherin-derived peptide, may be an effective tool for preventing the formation of F. nucleatum biofilms in the oral cavity.
(キーワード): Amino Acid Sequence / Animals / Antibodies, Bacterial / Bacterial Outer Membrane Proteins / Female / Fusobacterium nucleatum / Gene Deletion / Humans / Immunoglobulin A / Immunoglobulin G / Kinetics / Mice / Mice, Inbred C57BL / Molecular Sequence Data / Molecular Weight / Mucous Membrane / Protein Binding / Protein Interaction Mapping / Salivary Proteins and Peptides
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.01224-09
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20008529; ● Search Scopus @ Elsevier (PMID): 20008529; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.01224-09

(DOI: 10.1128/IAI.01224-09, PubMed: 20008529) 横山正明, 福井誠, 増田かなめ, 髙松夏子, 岡田寿朗, 武部裕光, 片岡宏介, 伊藤博夫 :
リアルタイム定量PCR(qPCR)法による唾液中の総細菌数の測定口腔清潔度の指標としての試み,
口腔衛生学会雑誌, Vol.59, No.3, 183-189, 2009年.

(要約): 口腔清潔度の指標としては,歯面への歯垢の付着度を測定する方法が一般的であるが,診査や記録等の人材的,時間的負担を要することから,集団歯科健診で用いられることは少ない.集団歯科健診においてう蝕や歯周疾患,口臭症のリスクを評価するために,簡便かつ有効な口腔清潔度の評価法の開発が望まれている.リアルタイム定量PCR(qPCR)法による唾液中の総細菌数の測定は,検体の採取や保管が容易で多検体の一括処理が可能であることから,本目的に合致する有望な測定法であると考えられる.本研究では,qPCR法を用いた唾液中の総細菌数の測定が口腔清潔度の評価法として使用できるかどうか検討を行った.5名のボランティアから,ブラッシング中断時と再開後の2回,刺激唾液を採取し,唾液中の総細菌数をqPCR法により測定したところ,Debris Index,O'LearyのPCR値との間に相関は示されなかったものの,ブラッシング再開後では,中断時に比べ,有意に減少した.81名の被験者から4ヵ月の間隔を空けて2回刺激唾液を採取し,唾液中の総細菌数をqPCR法により測定したところ,前後の間に有意な正の相関を認めた.以上の結果より,qPCR法によって唾液中の総細菌数を測定することが可能であることが示された.本法は非常に簡便であることから,今後,口腔清潔度を検討する分野において応用できる可能性が考えられる.
(キーワード): *Polymerase Chain Reaction
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679981008896

(CiNii: 1390282679981008896) Masaaki Yokoyama, Daisuke Hinode, Masami Yoshioka, Makoto Fukui, Shin-ichi Tanabe, Daniel Grenier and Hiro-O Ito :
Relationship between Campylobacter rectus and periodontal status during pregnancy.,
Oral Microbiology and Immunology, Vol.23, No.1, 55-59, 2008.

(要約): In a previous study, we showed that the growth of Campylobacter rectus is stimulated by the presence of female sex hormones in the culture medium. In the present study, we examined the relationship between C. rectus levels in the saliva and the periodontal status of pregnant women. Unstimulated whole saliva was collected from 22 pregnant and 15 non-pregnant women. Periodontal pocket depth (PD) and bleeding on probing (BOP) were recorded. A quantitative real-time polymerase chain reaction was performed to determine the concentrations of suspected periodontopathogenic bacteria in the saliva samples. In addition, the concentration of estradiol in the saliva samples was measured by enzyme immunoassay. The average age, number of teeth, and total number of bacteria in the saliva of subjects in both groups were similar. The percentage of sites with a PD = 4 mm and the salivary estradiol concentrations were significantly higher in pregnant women than in non-pregnant women. In addition, the percentage of BOP sites and the C. rectus levels in the saliva of the pregnant women tended to be higher than in non-pregnant women, although these differences were not statistically significant. There were positive correlations between C. rectus levels and estradiol concentrations, and between C. rectus levels and the percentage of sites with PD = 4 mm in the pregnant women. These results indicate that C. rectus levels are higher in the oral flora of pregnant women and that this may be associated with increased salivary estradiol concentrations. This may contribute to periodontal disease progression during pregnancy.
(キーワード): Actinobacillus actinomycetemcomitans / Adult / Age Factors / Campylobacter rectus / Colony Count, Microbial / Dentition / Estradiol / Estrogens / Female / Fusobacterium nucleatum / Gingival Hemorrhage / Humans / Periodontal Index / Periodontal Pocket / Porphyromonas gingivalis / Pregnancy / Pregnancy Complications / Prevotella intermedia / Saliva
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1399-302X.2007.00391.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18173799; ● Search Scopus @ Elsevier (PMID): 18173799; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1399-302X.2007.00391.x

(DOI: 10.1111/j.1399-302X.2007.00391.x, PubMed: 18173799) Mariko Naito, Yoshimi Suzukamo, Hiro-O Ito and Takeo Nakayama :
Development of the Japanese version of the Oral Impacts on Daily Performance (OIDP) scale: a pilot study.,
Journal of Oral Science, Vol.49, No.4, 259-264, 2007.

(要約): The Oral Impacts on Daily Performance (OIDP) is a well-known oral health-related QOL instrument used internationally. The aim of this study was to develop and test a Japanese version of the OIDP for use in interviews. Following an internationally established method, the OIDP scale was translated using standardized methodology that consisted of forward translation, pilot study and backward translation. A pilot study was carried out using the revised version with 47 local residents (range, 40-105 years). In the item analysis of this study, there were no missing values or ambiguous responses. Twenty-eight percent of the participants replied that they 'had discomfort caused by an oral health problem' in the 'eating' items. Cronbach's alpha coefficient was 0.77, indicating the internal consistency reliability of the questionnaire. Denture wearers had significantly higher OIDP scores in covariance analysis with age and sex as adjustment factors (P < 0.05). Based on the results, the questionnaire was re-evaluated. A back translation was produced and approved by the original author. Further assessment and validation of this scale is needed in a study with larger sample size.
(キーワード): Activities of Daily Living / Adult / Aged / Aged, 80 and over / Female / Humans / Japan / Male / Middle Aged / Oral Health / Pilot Projects / Quality of Life / Questionnaires / Sickness Impact Profile / Translations
(出版サイトへのリンク): ● Publication site (DOI): 10.2334/josnusd.49.259
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18195507; ● Search Scopus @ Elsevier (PMID): 18195507; ● Search Scopus @ Elsevier (DOI): 10.2334/josnusd.49.259

(DOI: 10.2334/josnusd.49.259, PubMed: 18195507) N Tanaka, S Fukuyama, T Fukuiwa, M Kawabata, Y Sagara, Hiro-O Ito, Y Miwa, T Nagatake, H Kiyono and Y Kurono :
Intranasal immunization with phosphorylcholine induces antigen specific mucosal and systemic immune responses in mice.,
Vaccine, Vol.25, No.14, 2680-2687, 2007.

(要約): Phosphorylcholine (PC) is a structural component of a wide variety of pathogens including Streptococcus pneumoniae and Haemophilus influenzae, and anti-PC immune responses are known to protect mice against invasive bacterial diseases. The present study tested the capability of PC as an intranasal plurispecific vaccine against upper airway infections. BALB/c mice immunized with intranasal PC-keyhole limpet hemocyanin (KLH) plus cholera toxin (CT) as a mucosal adjuvant showed increased PC-specific IgM in serum, IgA in nasal wash and saliva, and numbers of PC-specific nasal and splenic antibody producing cells. Enhanced production of IL-4 and IFN-gamma by CD4+ T cells indicated the participation of Th2- and Th1-type cells. Salivary IgA antibodies produced by intranasal immunization with PC-KLH plus CT reacted to most strains of S. pneumoniae and H. influenzae. Further we demonstrated that the clearance of S. pneumoniae and H. influenzae from the nasal tract was significantly enhanced by nasal immunization with PC-KLH and CT. Thus, intranasal vaccination to induce PC-specific immune responses might help to prevent upper airway infections caused by S. pneumoniae and H. influenzae.
(キーワード): Administration, Intranasal / Animals / Antibodies, Bacterial / Cholera Toxin / Female / Haemophilus influenzae / Hemocyanin / Immunity, Mucosal / Immunization / Immunoglobulin A, Secretory / Mice / Mice, Inbred BALB C / Phosphorylcholine / Streptococcus pneumoniae / Th1 Cells / Th2 Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.vaccine.2006.10.014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17270319; ● Search Scopus @ Elsevier (PMID): 17270319; ● Search Scopus @ Elsevier (DOI): 10.1016/j.vaccine.2006.10.014

(DOI: 10.1016/j.vaccine.2006.10.014, PubMed: 17270319) E Nagata, H Okayama, Hiro-O Ito, Y Yamashita, M Inoue and T Oho :
Serotype-specific polysaccharide of Streptococcus mutans contributes to infectivity in endocarditis.,
Oral Microbiology and Immunology, Vol.21, No.6, 420-423, 2006.

(要約): Streptococcus mutans and other viridans streptococci have been implicated as major etiological agents of infective endocarditis. The serotype-specific rhamnose-glucose polysaccharide (RGP) of S. mutans has several biological functions that appear to be essential for the induction of infective endocarditis. The aim of this study was to examine the contribution of RGP to the infectivity of S. mutans in infective endocarditis using a rat model. The RGP-defective mutant of S. mutans showed reduced ability to induce infective endocarditis compared to the parental strain. The ability of S. mutans to induce infective endocarditis was not consistent with the binding capacity of the organism to extracellular matrix proteins. The results suggest that S. mutans containing whole RGP is more virulent than the RGP-defective mutant, and the RGP has an important role for the induction of infective endocarditis by S. mutans.
(キーワード): Animals / Bacterial Adhesion / Endocarditis, Bacterial / Extracellular Matrix Proteins / Male / Polysaccharides, Bacterial / Protein Binding / Rats / Rats, Wistar / Serotyping / Species Specificity / Streptococcus mutans / Virulence Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1399-302X.2006.00317.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17064403; ● Search Scopus @ Elsevier (PMID): 17064403; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1399-302X.2006.00317.x

(DOI: 10.1111/j.1399-302X.2006.00317.x, PubMed: 17064403) Hiro-O Ito, K. Nokihara, S. Soutome and T. Ohyama :
Screening of peptides that inhibit bacterial binding to fibronectin using combinatorial peptide libraries.,
International Journal of Peptide Research and Therapeutics, Vol.12, No.3, 275-281, 2006.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10989-006-9030-7
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-33745834330

(DOI: 10.1007/s10989-006-9030-7, Elsevier: Scopus) E Nagata, H Okayama, Hiro-O Ito, I Semba, M Inoue and T Oho :
Experimental infective endocarditis induced by human supragingival dental plaque in rats.,
European Journal of Oral Sciences, Vol.113, No.6, 499-504, 2005.

(要約): Human dental plaque is thought to contribute to disease, not only in the oral cavity but also at other body sites. To investigate the pathogenicity of dental plaque in tissues remote from the mouth, we examined the ability of human supragingival dental plaque to induce infective endocarditis (IE) in rats. In total, 15 out of 27 catheterized rats survived after intravenous injections with human supragingival dental plaque suspensions containing 3 x 10(6) colony-forming units (CFU) of bacterial cells. In surviving rats, infected vegetations were formed in all except one rat. The microbial composition of the infected vegetations was different from that of the respective dental plaque inocula, with Streptococcus oralis comprising the majority of the isolates. In rats affected with endocarditis, the aortic sinus was filled with fibrinous vegetation containing bacteria. Inflammatory cells infiltrated the aortic valve, the aorta adjacent to the valve, and the cardiac muscles. The inoculation of catheterized rats with a cell suspension of S. oralis isolate (5 x 10(6) CFU) was not lethal but capable of inducing endocarditis in all animals. The results suggest that if dental plaque were introduced into the bloodstream, it could serve as a potent source of bacteria causing IE in humans.
(キーワード): Adult / Animals / Aortic Valve / Bacteremia / Biofilms / Dental Plaque / Disease Models, Animal / Endocarditis / Female / Humans / Male / Rats / Rats, Wistar / Sinus of Valsalva / Streptococcus oralis
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0722.2005.00258.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16324140; ● Search Scopus @ Elsevier (PMID): 16324140; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0722.2005.00258.x

(DOI: 10.1111/j.1600-0722.2005.00258.x, PubMed: 16324140) Y Miwa, Hiro-O Ito, M Inoue and T Oho :
Induction of immune response to Streptococcus pneumoniae by administration of oral viridans streptococci via phosphorylcholine determinant.,
FEMS Immunology and Medical Microbiology, Vol.43, No.3, 441-448, 2005.

(要約): Expression of the phosphorylcholine (PC) epitope was examined in 48 viridans streptococcal strains, including Streptococcus pneumoniae R36a as the positive control, and their immunogenicity to induce an S. pneumoniae-cross-reactive response was evaluated in mice. Thirteen strains were found to express the PC epitope, while no obvious association was found between the taxonomic categories and PC expression. Serum antibody responses to S. pneumoniae cells were induced in mice by intraperitoneal injection of the PC-positive, but not PC-negative, strains. The cross-reactive antibodies induced by non-pneumococcal oral streptococci were readily inhibited by free hapten PC. IgM was the sole isotype of the anti-pneumococcal and anti-PC antibodies, and the phenomenon of immunological memory was not observed. Since the anti-PC antibody is critically important for resistance against pneumococcal infection in mice, the present results indicate the possibility that PC-expressing oral commensal bacteria have a significant influence on the hosts' responsiveness to S. pneumoniae.
(キーワード): Animals / Antibodies, Bacterial / Cross Reactions / Epitopes / Female / Humans / Immunoglobulin Isotypes / Mice / Mice, Inbred BALB C / Mouth / Phosphorylcholine / Streptococcus pneumoniae / Viridans Streptococci
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.femsim.2004.11.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15708320; ● Search Scopus @ Elsevier (PMID): 15708320; ● Search Scopus @ Elsevier (DOI): 10.1016/j.femsim.2004.11.001

(DOI: 10.1016/j.femsim.2004.11.001, PubMed: 15708320) Hidehito Okayama, Emi Nagata, Hiro-O Ito, Takahiko Oho and Masakazu Inoue :
Experimental Abscess Formation Caused by Human Dental Plaque,
Microbiology and Immunology, Vol.49, No.5, 399-405, 2005.

(要約): Human dental plaque consists of a wide variety of microorganisms, some of which are believed to cause systemic infections, including abscesses, at various sites in the body. To confirm this hypothesis experimentally, we examined the abscess-forming ability of native dental plaque in mice, the microbial features of the infectious locus produced by the plaque, and the anti-phagocytic property of microbial isolates. Aliquots of a suspension of supragingival dental plaque containing 6 x 10(6) colony-forming unit of bacteria were injected subcutaneously into the dorsa of mice. Abscess formation was induced in 76 of 85 mice using ten different plaque samples. Thirteen microorganisms were isolated from pus samples aspirated from abscess lesions. The microbial composition of pus, examined in 17 of 76 abscesses, was very simple compared to that of the plaque sample that had induced the abscess. The majority of the isolates belonged to the Streptococcus anginosus group, normally a minor component of plaque samples. S. anginosus was the most frequently detected organism and the most prevalent in seven abscesses, and Streptococcus intermedius and Streptococcus constellatus were predominant in one and three abscess samples, respectively. Each isolate of S. anginosus group produced abscesses in mice, and heat-treated supragingival dental plaque influenced the abscess-forming ability of S. anginosus isolate. These isolates possessed a high antiphagocytic capacity against human polymorphonuclear leukocytes. Our results suggest that human supragingival dental plaque itself is a source of the infectious pathogens that cause abscess formation.
(キーワード): Dental Plaque / Abscess / Streptococcus anginosus group
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15905601; ● Search Scopus @ Elsevier (PMID): 15905601

(PubMed: 15905601) Hiro-O Ito, Sakiko Soutome, Kiyoshi Nokihara and Masakazu Inoue :
Identification and characterization of bacterial-binding property in the type III repeat domain of fibronectin,
Biochemical and Biophysical Research Communications, Vol.320, No.2, 347-353, 2004.

(要約): To characterize fibronectin binding with Granulicatella adiacens, a causative agent of infective endocarditis, monoclonal antibodies were generated against human fibronectin and selected for their capacity to inhibit the fibronectin binding of the organism. Thermolysin and lysyl-endopeptidase digests of fibronectin were characterized by Western blot. The epitope of inhibitory monoclonal antibody was found in the central portion of fibronectin known as the cell-binding domain, and not in the N-terminal portion known to be the binding region of most microbial species, e.g., Staphylococcus aureus and Streptococcus pyogenes. While these two species could bind to both the N-terminal and central portion, Escherichia coli and G. adiacens bind only to the latter. Excess amounts of free fibronectin in the solution inhibited the bacterial adherence to the N-terminal fibronectin fragment, but not to the central region, thereby suggesting the central region plays a significant role for in vivo bacterial colonization in the presence of high concentrations of soluble fibronectin.
(キーワード): Animals / Antibodies, Monoclonal / Epitope Mapping / Female / Fibronectins / Lactobacillaceae / Mice / Mice, Inbred BALB C / Repetitive Sequences, Amino Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2004.05.170
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15219833; ● Search Scopus @ Elsevier (PMID): 15219833; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2004.05.170

(DOI: 10.1016/j.bbrc.2004.05.170, PubMed: 15219833) 水流和徳, 伊藤博夫, 富田浩一, 瀬戸口尚志, 門川明彦, 川島清美, 西原一秀, 瀬戸上康子, 重田浩樹, 佐藤強志, 石神哲郎, 長岡英一 :
本学歯学部における OSCE トライアルの実施方法について,
日本歯科医学教育学会雑誌, Vol.19, No.1, 160-169, 2003年.

(キーワード): OSCE / OSCE traial / early exposure / motivation / questionnaire survey
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1573387449774800384

(CiNii: 1573387449774800384) 石川久美子, 古市保志, 伊藤博夫, 四元幸治, 下津昭洋, 山下皓三, 児島正明, 和泉雄一 :
歯周病罹患状況と臨床検査値および歯周病原性細菌に対する血清抗体価の変化とその関連, --- 同じ被験者を対象とした3年間の調査結果 ---,
歯周組織，臨床検査値および血清抗体価に関する3年間の追跡調査, 394-401, 2003年.

(キーワード): 継続調査 / CPITN / 歯周疾患 / Porphyromonas gingivalis / Actionobacillus actinomycetemcomitans

Hiro-O Ito, Sakiko Soutome and Masakazu Inoue :
Inhibition of fibronectin binding of some bacterial cells by subtle pH increase within the physiological range,
Journal of Microbiological Methods, Vol.55, No.1, 29-34, 2003.

(要約): The fibronectin (Fn)-binding ability of microorganisms is considered to be involved in their pathogenicities. Granulicatella adiacens, a member of the oral flora and a causative agent of culture-negative infective endocarditis, showed nearly maximum binding to immobilized Fn at pH 7.2 but greatly reduced binding at a slightly higher pH 7.4 and almost no binding at pH 7.6 in the presence of physiological concentration of NaCl (0.15 M). A similar pH-sensitive Fn-binding property was noted with Escherichia coli and Abiotrophia defectiva, but not with Streptococcus pyogenes nor Staphylococcus aureus. In contrast, bindings to laminin and fibrinogen observed for some of these strains were unaffected by the same pH changes. This fastidious pH-dependency of Fn-binding abilities of some bacteria warns that the pH condition must be seriously considered in the in vitro assay of bacterial adherence to fibronectin.
(キーワード): Adhesion / Fi bronectin / pH / Collagen / Nutritionally variant sterptococci(NVS)
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0167-7012(03)00111-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14499992; ● Search Scopus @ Elsevier (PMID): 14499992; ● Search Scopus @ Elsevier (DOI): 10.1016/S0167-7012(03)00111-8

(DOI: 10.1016/S0167-7012(03)00111-8, PubMed: 14499992) Yasushi Furuichi, Akihiro Shimotsu, Hiro-O Ito, Yukari Namariyama, Yukiharu Yotsumoto, Youishi Hino, Yukie Mishige, Masakazu Inoue and Yuichi Izumi :
Associations of Periodontal Status with General Health Conditions and Aerum Antibody Titers for Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans,
Journal of Periodontology, Vol.74, No.10, 1491-1497, 2003.

(要約): The purpose of the present study was to evaluate associations of periodontal status with general health conditions and with serum antibody titers for Porphyromonas gingivalis (Pg) and Actinobacillus actinomycetemcomitans (Aa) in a Japanese population. Subjects (1,314) aged 40 years or older were recruited for the study. Their periodontal status was evaluated using the community periodontal index of treatment needs (CPITN). Records from general health examinations were used for the present analyses, including data on age, gender, body mass index, systolic and diastolic blood pressure (SBP/DBP); levels of serum alkaline phosphatase, total cholesterol, high-density lipoprotein cholesterol, triglyceride, fasting blood glucose, and C-reactive protein (CRP); counts of white blood cells (WBC) and platelets; smoking habit; and electrocardiogram recordings. Serum IgG antibody titers for Pg fimbrial antigens (IgG.Pg.Fim.), Pg whole-cell antigens (IgG.Pg.whol.), and Aa whole-cell antigens (IgG.Aa.whol.) were determined using enzyme-linked immunosorbent assay. The subjects were divided, according to their maximum CPITN codes, into four periodontal groups: max.CPITN-0/1/2, -3, -4, or -MS (missing sextant). Multivariable logistic regression analyses revealed significant associations of the max.CPITN-4 subjects who were males (odds ratio, OR: 2.03) or smokers (OR: 1.73), or who had high diastolic blood pressure (OR: 1.27), high WBC (OR: 1.24), high serum CRP level (OR: 2.39) and high serum IgG.Pg.Fim. (OR: 1.17), IgG.Pg.whol. (OR:1.54), and IgG.Aa.whol. (OR: 1.19) titers. Significant associations between periodontal status and several health conditions were found in the adult population examined, including gender, smoking habit, diastolic blood pressure, white blood cell counts, C-reactive protein, and serum IgG antibodies to P. gingivalis fimbriae, IgG P. gingivalis whole cell, and IgG A. actinomycetemcomitans whole cell titers.
(キーワード): Actinobacillus actinomycetemcomitans / antibody titer / health / Japanese / Periodontitis/microbiology
(出版サイトへのリンク): ● Publication site (DOI): 10.1902/jop.2003.74.10.1491
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14653396; ● Search Scopus @ Elsevier (PMID): 14653396; ● Search Scopus @ Elsevier (DOI): 10.1902/jop.2003.74.10.1491

(DOI: 10.1902/jop.2003.74.10.1491, PubMed: 14653396) Hiro-O Ito, Toshihiro Nakashima, Takanori So, Masato Hirata and Masakazu Inoue :
Immunodominance of conformation-dependent B-cell epitopes of protein antigens,
Biochemical and Biophysical Research Communications, Vol.308, No.4, 770-776, 2003.

(要約): Immunodominance of conformational epitopes over linear ones in four proteins was quantified making use of the B-cell hybridoma technology. The proteins were immunized in their native forms into BALB/c mice, and clonal frequencies of B-cell hybridomas that produce antibodies to the native and denatured forms were determined, using ELISA and immunoblotting. All 16 monoclonal antibodies (mAbs) to Porphyromonas gingivalis fimbria were suggested to recognize conformational epitopes expressed by the oligomer. Ten out of 14 mAbs to Serratia marcescens fimbria and 13 of 15 mAbs to hen lysozyme were also specific to their conformational epitopes. In contrast, all 18 mAbs to a surface protein of Streptococcus mutans, termed PAc, reacted to both the native and denatured forms, thereby indicating the immunodominance of linear epitopes in this protein. The results suggest that B-cell epitopes of proteins possessing stable tertiary or quaternary structures are predominantly expressed by the higher-order structures.
(キーワード): Monoclonal antibodies / B-cell cpitope / Higher-order structure / Immunodominance / Protein antigens / Hen-egg Iysozyme / Porphyromonas gingivalis / Streptococcus mutans / Serratia marcescens
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0006-291X(03)01466-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12927785; ● Search Scopus @ Elsevier (PMID): 12927785; ● Search Scopus @ Elsevier (DOI): 10.1016/S0006-291X(03)01466-9

(DOI: 10.1016/S0006-291X(03)01466-9, PubMed: 12927785) Takanori So, Hiro-O Ito, Masato Hirata, Tadashi Ueda and Taiji Imoto :
Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses,
Immunology, Vol.104, No.3, 259-268, 2001.

(要約): It is important to identify characteristics that confer on proteins the potential to induce allergenic sensitization and allergenic disease. Protein allergens carry T-cell epitopes that are capable of inducing a type 2 T helper (Th2) cell response. There is limited information regarding factors that govern the allergenicity of proteins. We previously reported that a decrease in the conformational stability of hen-egg lysozyme (HEL) enhanced its capacity to activate HEL-specific T cells owing to the increased susceptibility to intracellular antigen processing. To determine whether the conformational stability of HEL makes for a critical contribution to allergenic sensitization in vivo, we immunized BALB/c mice with HEL derivatives of different conformational stability, but which retained a similar three-dimensional structure. The magnitude of in vivo T-cell responses, evaluated by ex vivo proliferative responses of lymph node T cells from mice primed with various HEL derivatives, was inversely correlated with conformational stability, as was interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) production by splenic T cells in response to HEL. Immunization of the least stable derivative led to a potent IL-4 response and to immunoglobulin E (IgE) antibody production. We propose that the intrinsic allergenicity of proteins can be determined by the degree of conformational stability.
(キーワード): Animals / Antigen Presentation / Antigens / Epitopes, T-Lymphocyte / Female / Immunoglobulin E / Interferon-gamma / Interleukin-4 / Mice / Mice, Inbred BALB C / Muramidase / Protein Conformation / Structure-Activity Relationship / Th1 Cells / Th2 Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.1365-2567.2001.01314.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11722640; ● Search Scopus @ Elsevier (PMID): 11722640; ● Search Scopus @ Elsevier (DOI): 10.1046/j.1365-2567.2001.01314.x

(DOI: 10.1046/j.1365-2567.2001.01314.x, PubMed: 11722640) Yoko Sakai, Hidetoshi Shimauchi, Hiro-O Ito, Masahiro Kitamura and Hiroshi Okada :
Porphyromonas gingivalisspecific lgG subclass antibody levels as immunological risk indicators of periodontal bone loss,
Journal of Clinical Periodontology, Vol.28, No.9, 853-859, 2001.

(要約): It has been well demonstrated a positive association between the magnitude of host antibody response and periodontal disease status. Previous studies also reported that Porphyromonas gingivalis-specific IgG subclass antibodies were elevated in sera from adult periodontitis patients. However, the rôle and the association of these IgG subclass antibodies to the development of periodontal diseases are poorly understood. The aim of present investigation was to examine the relation of serum IgG subclass antibody levels and alveolar bone loss in treated and untreated periodontitis patients. Serum samples were taken from 20 treated and maintained periodontitis patients (SPT patients), 30 untreated patients and 19 periodontally healthy subjects. We determined the IgG subclass antibody titers to P. gingivalis whole cells using an enzyme-linked immunosorbent assay (ELISA). Subgingival plaque samples were taken from the mesio-buccal surface of 6 randomly selected teeth of SPT patients and evaluated for the presence of P. gingivalis by immunofluorescence microscopy. Clinical measurements were also taken including full mouth intraoral radiographs to measure interproximal alveolar bone loss at baseline (BLS1) and at a 5-year recall visit in the SPT patients (BLS2). Our results indicated that both patient groups had detectable levels of IgG1, IgG2, and IgG4. Significantly higher IgG1 was observed in both patient groups compared to the healthy subjects. The untreated patients also exhibited significantly elevated IgG2 response (p<0.05). The mean IgG4 level of the SPT patients was significantly higher compared to the other subject group (p<0.05). A statistically significant positive correlation between IgG2 levels and changes in bone levels (DeltaBLS: BLS2-BLS1) was seen in the SPT patients (p<0.001). SPT patients with high IgG2 and low IgG4 showed greater bone loss than those with low IgG2 and high IgG4 (p<0.05), although the mean prevalence of P. gingivalis in the 2 groups did not differ significantly. Our data suggest that the prolonged IgG2 response after periodontal treatment may be indicative of recurrent or persistent periodontal destruction.
(キーワード): Porhyomonas gingivalis / lgG subclass / periodontal bone destruction / risk indicator / supporting periodontal therapy
(出版サイトへのリンク): ● Publication site (DOI): 10.1034/j.1600-051x.2001.028009853.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11493355; ● Search Scopus @ Elsevier (PMID): 11493355; ● Search Scopus @ Elsevier (DOI): 10.1034/j.1600-051x.2001.028009853.x

(DOI: 10.1034/j.1600-051x.2001.028009853.x, PubMed: 11493355) Y Furuichi, Hiro-O Ito, Y Izumi, T Matsuyama, Y Yotsumoto, Y Mishige, M Kojima, K Yamashita and M Inoue :
Periodontal status and serum antibody titers for Porphyromonas gingivalis fimbriae in a rural population in Japan,
Journal of Clinical Periodontology, Vol.28, No.3, 264-269, 2001.

(要約): The present study was undertaken to assess the periodontal status of a rural Japanese population and to study the correlation between the periodontal status and the serum antibody titers for Porphyromonas gingivalis (Pg) fimbriae. A total of 236 individuals were examined for their periodontal conditions by the use of the community periodontal index for treatment needs (CPITN), and serum antibody titers for Pg fimbriae in their peripheral blood samples were evaluated using the enzyme-linked immunosorbent assay. There was a substantially larger proportion of edentulous subjects in the age group older than 60 years. The remaining teeth were 24.1, 23.2, 11.1 and 10.1 per person in the 40-49, 50-59, 60-69 and > or = 70 age groups, respectively. The % of sextants with a CPITN code of missing sextant (MS) increased towards elderly and reached >60% in the age group of > or = 70 years, as the % of the CPITN 2, 1 or 0 sextant decreased. The % of CPITN 4 and 3 sextants did not differ between different age groups and were about 6-8% and 15-20%, respectively. The % of CPITN 1 or 0 sextants was higher in female subjects than in male subjects in the 60-69 and > or = 70 age groups, while the % of CPITN 4 or 3 sextants was higher in male subjects than in female subjects in all age groups. There was no significant difference between various age groups in the mean serum antibody titers for Pg fimbriae. The mean anti-Pg fimbriae antibody titers was significantly higher for the subjects with a maximum CPITN code 4 (max.-CPITN 4 subject) than for the subjects with lower maximum CPITN codes. The antibody titers varied extensively among the max.-CPITN 4 or 3 subjects, but not among the max.-CPITN 2/1/0 or MS subjects. The present study demonstrated that tooth loss is a remarkable event in elderly subjects and that oral prophylaxis and mechanical debridement should be mandatory in the population examined. It was also demonstrated that the serum antibody titers against Pg fimbriae could be useful for screening individuals with moderate to severe periodontitis.
(キーワード): antibody / CPITN / epidemiology / fimbriae / periodontitis / Porphyromonas gingivalis
(出版サイトへのリンク): ● Publication site (DOI): 10.1034/j.1600-051x.2001.028003264.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11284541; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035287493

(DOI: 10.1034/j.1600-051x.2001.028003264.x, PubMed: 11284541, Elsevier: Scopus) A Yoshida, Y Nakano, Y Yamashita, T Oho, Hiro-O Ito, M Kondo, M Ohishi and T Koga :
Immunodominant Region of Actinobacillus actinomycetemcomitans 40-kilodalton Heat Shock Protein in Patients with Rheumatoid Arthritis,
Journal of Dental Research, Vol.80, No.1, 346-350, 2001.

(要約): Bacterial heat shock proteins have been implicated in the pathogenesis of several diseases, and the immunological relationship between rheumatoid arthritis (RA) and Escherichia coli DnaJ has been reported. Since there are similarities in the tissue destruction process of RA and periodontitis, we examined the reactivities of antibodies in sera from RA patients to the DnaJ protein from Actinobacillus actinomycetemcomitans. An enzyme-linked immunosorbent assay showed that IgG titers to the N-terminal conservative region of the DnaJ are significantly higher in RA patients compared with the healthy controls (p < 0.05). Furthermore, we examined IgG titers of disease controls to determine the specificity of the immune responses to this region in RA patients. The difference between RA and infectious disease patients was also significant (p < 0.05). These results suggest that the N-terminal region of DnaJ from A. actinomycetemcomitans may contribute to the etiologic analysis of RA.
(キーワード): Actinobacillus actinomycetemcomitans / DnaJ / heat shock protein(HSP) / periodontitis / rheumatoid arthritis(RA)
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/00220345010800010901
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11269727; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034989792

(DOI: 10.1177/00220345010800010901, PubMed: 11269727, Elsevier: Scopus) Hiroko Ito, Kimie Okimoto, 伊藤博夫, Yoshihiro Terada :
Effect of Denture Application on senile patients,
Dentistry in Japan, Vol.36, 54-58, 2000年.

(キーワード): Mastication / Immunological function / Elderly

Yasuko Okada, Katsuhiro Kitada, Masaru Takagaki, Hiro-O Ito and Masakazu Inoue :
Endocardiac infectivity and binding to extracellular matrix proteins of oral Abiotrophia species,
FEMS Immunology and Medical Microbiology, Vol.27, No.3, 257-261, 2000.

(要約): Microorganisms of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are members of the oral flora and often isolated from patients with endocarditis, but pathogenicity of oral Abiotrophia species has not been examined yet. In this study, 17 strains isolated from healthy human oral cavities and 7 reference strains (all derived from patients with endocarditis) of Abiotrophia spp. were tested for their abilities to cause infections in damaged heart tissues in catheterized rats and to adhere to extracellular matrix proteins in vitro. The reference strains of A. defectiva and A. adiacens showed high infectivities in the rats. Four oral isolates of these two species showed similarly high infectivities and three had moderate infectivities. Most of 10 oral strains of A. para-adiacens and A. elegans were found to be generally less infective. The highly infective A. adiacens strains showed markedly high fibronectin-binding capacity, suggesting a possible relationship between the fibronectin-binding capacity and damaged heart tissue infectivity of the Abiotrophia species. A. defectiva strains which were also highly infective had moderate levels of binding to fibronectin and other extracellular matrix proteins. Most of A. para-adiacens and A. elegans strains showed low or negligible binding capacities to any extracellular matrix proteins tested.
(キーワード): Animals / Bacterial Adhesion / Colony Count, Microbial / Endocarditis, Bacterial / Endocardium / Extracellular Matrix Proteins / Gram-Positive Bacterial Infections / Heart Valves / Humans / Male / Mouth / Rats / Rats, Wistar / Streptococcaceae
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0928-8244(99)00193-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10683471; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033973931

(DOI: 10.1016/S0928-8244(99)00193-5, PubMed: 10683471, Elsevier: Scopus) Takanori So, Hiro-O Ito, Yoshiyuki Tsujihata, Masato Hirata, Tadashi Ueda and Taiji Imoto :
The molecular weight ratio of monomethoxypolyethylene glycol (mPEG) to protein determines the immunotolerogenicity of mPEG proteins,
Protein Engineering, Vol.12, No.8, 701-705, 1999.

(要約): Immunotolerogenic activity of monomethoxypolyethylene glycol- (mPEG) conjugated proteins is a beneficial property in protein pharmaceutics. However, procedures for the preparation of tolerogenic mPEG proteins have not yet been defined. We prepared mPEG proteins with different mPEG contents using three proteins, hen egg lysozyme, ovalbumin and bovine gamma globulin, and their tolerogenicities to antigen-specific T and B cell responses were examined. We found the most appropriate ratio of tolerance induction to be 1.5-2.0, which is the molecular weight ratio of conjugated total mPEGs to protein. This value may assist in the preparation of tolerogenic mPEG proteins.
(キーワード): Animals / B-Lymphocytes / Cattle / Drug Design / Immune Tolerance / Mice / Mice, Inbred BALB C / 分子量 (molecular weight) / Polyethylene Glycols / タンパク質工学 (protein engineering) / Proteins / Tリンパ球 (T lymphocytes)
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/protein/12.8.701
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10469832; ● Search Scopus @ Elsevier (PMID): 10469832; ● Search Scopus @ Elsevier (DOI): 10.1093/protein/12.8.701

(DOI: 10.1093/protein/12.8.701, PubMed: 10469832) T So, Hiro-O Ito, M Hirata, Tadashi Ueda and Taiji Imoto :
Extended blood half-life of monomethoxypolyethylene glycol-conjugated hen lysozyme is a key parameter controlling immunological tolerogenicity,
Cellular and Molecular Life Sciences, Vol.55, No.8,9, 1187-1194, 1999.

(要約): The blood half-life of a protein is prolonged by conjugating a protein with a linear amphiphilic polymer, monomethoxypolyethylene glycol (mPEG). The conjugation gives a protein immunotolerogenicity; hence, it is likely that the long half-life is crucial for the tolerogenicity. We prepared a tolerogenic mPEG conjugate of hen egg lysozyme (mPEG1.5-HEL), which is conjugated 1.5-fold the molecular weight of mPEG against that of HEL, and evaluated the relationship between in vivo stability and the tolerogenicity. mPEG1.5-HEL retained immunogenicity to prime HEL-specific T cell and antibody responses and had a long blood half-life, more than 27 times that of native HEL. The tolerant state was maintained as long as mPEG1.5-HEL was detected in sera. With a decrease in the blood mPEG1.5-HEL level, the tolerant state returned gradually to the responsive state; however, reinjection of mPEG1.5-HEL again restored the tolerance. Thus, the extended blood half-life of HEL by mPEG conjugation is probably vital for establishing and maintaining the tolerant states.
(キーワード): Immunological tolerance / monomethoxypolyethylene glycol / extended blood half-life / hen egg lysozyme / immunochemistry
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s000180050365
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10442096; ● Search Scopus @ Elsevier (PMID): 10442096; ● Search Scopus @ Elsevier (DOI): 10.1007/s000180050365

(DOI: 10.1007/s000180050365, PubMed: 10442096) Manabu Yanagita, Takachika Hiroi, Noriko Kitagaki, Shigeyuki Hamada, Hiro-O Ito, Hidetoshi Shimauchi, Shinya Murakami, Hiroshi Okada and Hiroshi Kiyono :
Nasopharyngeal-Associated Lymphoreticular Tissue (NALT) Immunity: Fimbriae-Specific Th1 and Th2 Cell-Regulated IgA Responses for the Inhibition of Bacterial Attachment to Epithelial Cells and Subsequent Inflammatory Cytokine Production,
The Journal of Immunology, Vol.162, No.6, 3559-3565, 1999. Hiro-O Ito, Takanori So, Masato Hirata, T Koga, Tadashi Ueda and Taiji Imoto :
Tolerogenic activity of polyethylene glycol-conjugated lysozyme distinct from that of the native counterpart,
Immunology, Vol.93, No.2, 200-207, 1998. Toshihiasa Kawai, Hiro-O Ito, Nobuo Sakato and Hiroshi Okada :
A novel approach for detecting an immunodominant antigen of Porphyromonas gingivalis in diagnosis of adult periodontitis,
Clinical and Diagnostic Laboratory Immunology, Vol.5, No.1, 11-17, 1998. Takanori So, Hiro-O Ito, Toshitaka Koga, Sanae Watanabe, Tadashi Ueda and Taiji Imoto :
Depression of T-cell Epitope Generation by Stabilizing Hen Lysozyme,
The Journal of Biological Chemistry, Vol.272, No.51, 32136-32140, 1997. Hiro-O Ito, T So, T Ueda, T Imoto and T Koga :
Prevention of collagen-induced arthritis (CIA) by treatment with polyethylene glycol-conjugated type II collagen; distinct tolerogenic property of the conjugated collagen from the native one,
Clinical and Experimental Immunology, Vol.108, No.2, 213-219, 1997.

(キーワード): polyethylene glycol / type 2 collagen / collagen-induced arthritis / tolerance

Hiro-O Ito, M Hirata and T Koga :
Hen egg-white lysozyme inhibits biological activities of lipopolysaccharides from periodontopathic bacteria.,
Journal of Periodontal Research, Vol.32, No.3, 295-299, 1997.

(キーワード): hen egg-white lysozyme / bacterial lipopolysaccharides / periodontitis / osteoclasts

Yoshiko Murakami, Yoshihisa Yamashita, Yoshio Nakano, Hiro-O Ito, Hao Yu and Toshihiko Koga :
Role of the Charged Tail in Localization of a Surface Protein Antigen of Streptococcus mutans,
Infection and Immunity, Vol.65, No.4, 1531-1535, 1997. Hiro-O Ito, T Ueda, Y Hashimoto, T Imoto and T Koga :
Quaternary structure-dependent idiotope and antigen binding of a monoclonal antibody specific for conformational epitope on type II collagen,
Cellular and Molecular Life Sciences, Vol.53, No.1, 51-60, 1997.

(要約): We previously generated a monoclonal antibody (mAb) against a putative pathogenic epitope on native type II collagen (CII) for the induction of collagen-induced arthritis in mice (mAb1), and an anti-idiotypic mAb which appears to possess the internal image of the CII epitope (mAb2). In the present study, the structural basis of the antigen/mAb1 and mAb1/mAb2 interactions was examined. When partially SH-reduced mAb1 was analysed on Western blots, only fragments containing both heavy (H) and light (L) chains were recognized by mAb2. When mAb2 was partially SH-reduced, only fragments containing both H and L chains were recognized by mAb1. H and L chains were separated from mAb1 in a reduced, denatured condition, and each chain and a mixture of the two were refolded. mAb2 reacted specifically to the renatured whole IgG molecule of mAb1, but not to the refolded L or to H chains. Recombinant single chain Fv (scFv) generated from mAb1 and mAb2 had properties of the original mAbs, whereas genetically constructed chimeric scFvs, consisting of VH from mAb1 and an irrelevant VL. or VL of mAb1 and an irrelevant VH. did not react either to CII or to mAb2. Thus, interactions among CII, mAb1 and mAb2 appear to depend on quaternary structures containing different protein subunits. These observations support the internal image property of the mAb2. In addition, this dependency on quaternary structure for recognition of proteins may also be relevant to other protein-protein interactions.
(キーワード): Protein-protein interaction / higher-order structure / anti-idiotypie antibody / type 2 collagen / autoimmune arthritis
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/PL00000579
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9117997; ● Search Scopus @ Elsevier (PMID): 9117997; ● Search Scopus @ Elsevier (DOI): 10.1007/PL00000579

(DOI: 10.1007/PL00000579, PubMed: 9117997) Hiro-O Ito, T Shuto, H Takada, T Koga, Y Aida and M Hirata :
Lipopolysaccharides from Porphyromonas gingivalis, prevotella intermedia and Actinobacillus actinomycetemcomitans promote osteoclastic differentiation in vitro,
Archives of Oral Biology, Vol.41, No.5, 439-444, 1996.

(キーワード): bacterial lipopolysaccharides / osteoclasts / periodnatal diseases
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/0003-9969(96)00002-7
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/0003-9969(96)00002-7

(DOI: 10.1016/0003-9969(96)00002-7) Takanori So, Hiro-O Ito, Toshitaka Koga, Tadashi Ueda and Taiji Imoto :
Reduced immunogenicity of monomethoxypolyethylene glycol-modified lysozyme for activation of T cells,
Immunology Letters, Vol.49, No.1,2, 91-97, 1996.

(キーワード): Monomethoxypolyethylene glycol / Hen egg-white lysozyme / Immunogenicity / T cell activation / Antigen presentation

酒井洋子, 伊藤博夫, 岡田宏 :
抗 Porphyromonas gingivalis 抗体の IgG サブクラスと歯周炎の病態,
日本歯周病学会会誌, Vol.36, No.4, 871-883, 1994年.

(キーワード): 抗体 / IgG サブクラス / Porphyromonas gingiivalis / 歯周炎

Hiro-O Ito, J Morizet, L Coulombel and M Stanislawski :
T cell depletion of human bone marrow using an oxidase-peroxidase enzyme immunotoxin,
Bone Marrow Transplantation, Vol.6, No.6, 395-398, 1990. Hiro-O Ito, V Rousseau, M Goavec, F Beaujean, J Morizet and M Stanislawski :
Enhanced in vitro cytotoxicity of 1,3-bis-(2-chloroethyl)-1-nitrosourea or buthionine sulfoximine combined with a reactive oxygen-generating enzyme immunotoxin,
Cancer Letters, Vol.49, No.2, 169-173, 1990.

(キーワード): cytotoxicity / leukemia cells / 1,3-bis(2-chloroethy1)-1-nitrosourea / reactive oxygen-generating enzyme immunotoxin

岡田年似, 原田泰, 伊藤博夫, 野崎剛徳, 恵比須繁之, 木村重信, 岡田宏 :
Actinomyces viscosus T14V 株の示す多クローン性B細胞活性化, --- ヒト末梢血リンパ球の応答性 ---,
日本歯周病学会会誌, Vol.31, No.4, 1072-1080, 1989年.

(キーワード): 多クローン性B細胞活性化(PBA) / Actinomyces viscosus T14V / ヒト末梢血リンパ球 / T細胞依存性PBA / 超音波破砕上清

Stanislawski Marc, Rousseau Veronique, Goavec Martine and Hiro-O Ito :
Immunotoxins Containing Glucose Oxidase and Lactoperoxidase with Tumoricidal Properties: in Vitro Killing Effectiveness in a Mouse Plasmacytoma Cell Model,
Cancer Research, Vol.49, No.20, 5497-5504, 1989. Hiro-O Ito, J Morizet, L Coulombel, M Goavec, V Rousseau and A Bernard :
An immunotoxin system intended for bone marrow purging composed of glucose oxidase and lactoperoxidase coupled to monoclonal antibody 097,
Bone Marrow Transplantation, Vol.4, No.5, 519-527, 1989. H Okada, Y Shimabukuro, Y Kassai, Hiro-O Ito, Takashi Matsuo, S Ebisu and Y Harada :
The Function of Gingival Lymphocytes on the Establishment of Human Periodontitis,
Advances in Dental Research, Vol.2, No.2, 364-367, 1988. Yasushi Harada, Hiro-O Ito, Yasuo Miki, Shigeyuki Ebisu and Hiroshi Okada :
Polyclonal induction of IgG antibody forming cells by stimulation with Actinomyces viscosus T14V,
Journal of Periodontal Research, Vol.23, No.2, 122-126, 1988. Hiro-O Ito, Yasushi Harada, Takashi Matsuo, Shigeyuki Ebisu and Hiroshi Okada :
Possible role of T cells in the establishment of IgG plasma cell-rich periodontal lesion--Augmentation of IgG synthesis in the polyclonal B cell activation response by autoreactive T cells,
Journal of Periodontal Research, Vol.23, No.1, 39-45, 1988. Hiroshi Okada, Hiro-O Ito and Yasushi Harada :
T-cell requirement for establishment of the IgG-dominant B-cell lesion in periodontitis,
Journal of Periodontal Research, Vol.22, No.3, 187-189, 1987. 伊藤博夫 :
T細胞による多クローン性B細胞活性化の修飾, --- 特に，多クローン性 IgG 産生の増強について ---,
日本歯周病学会会誌, Vol.29, No.1, 22-36, 1987年.

(キーワード): 歯周炎 (periodontal disease) / Polyclonal B cell activation (PBA) / Autoreactive T cell / la antigen / Syngeneic mixed lymphocyte reaction

MISC

三木かなめ, 玉木直文, 伊藤博夫 :
天然ハーブの有用性ー歯科領域を中心に,
Journal of Oral Health and Biosciences, Vol.32, No.1, 16-25, 2019年.

(キーワード): 天然ハーブ / 歯周病 (periodontitis) / 全身疾患 / キャッツクロー
(徳島大学機関リポジトリ): ● Metadata: 113665
(出版サイトへのリンク): ● Publication site (DOI): 10.20738/johb.32.1_16
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390564238105416064; ● Search Scopus @ Elsevier (DOI): 10.20738/johb.32.1_16

(徳島大学機関リポジトリ: 113665, DOI: 10.20738/johb.32.1_16, CiNii: 1390564238105416064)

総説・解説

葭原明弘安細敏弘, 伊藤博夫, 佐々木健山本龍生 :
日本人における歯周病のセルフレポートに関する文献レビュー,
口腔衛生学会雑誌, Vol.67, No.3, 196-200, 2017年7月. 片岡宏介, 土居貴士, 神光一郎, 上根昌子, 伊藤博夫, 落合(栗田) 智子, 三宅達郎 :
樹状細胞を活性化する経鼻アジュバントの開発-感染症・NCD制御を目指す粘膜ワクチン-,
口腔衛生学会雑誌, Vol.67, No.2, 2-10, 2017年6月.

(キーワード): 樹状細胞 (dendritic cell) / 経鼻アジュバント / 粘膜ワクチン / 感染症・NCDs
(徳島大学機関リポジトリ): ● Metadata: 117497
(出版サイトへのリンク): ● Publication site (DOI): 10.5834/jdh.67.1_2
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.5834/jdh.67.1_2

(徳島大学機関リポジトリ: 117497, DOI: 10.5834/jdh.67.1_2) 伊藤博夫 :
歯周病予防と生活習慣，そして生活習慣病予防と歯周病,
四国医学雑誌, Vol.65, No.5,6, 117-122, 2009年12月.

(要約): The onset and progression of periodontal disease is closely associated with patients' lifestyles and habits, such as smoking, diet, stresses, etc. ; it is therefore considered to be a lifestyle-related disease. It is now listed as one of the complications of diabetes mellitus, as well as disorders of the retina, kidney, nervous system, heart, and brain. In addition to the concept that lifestyles and lifestyle-related diseases are causative factors of periodontal disease, this disease has now been revealed to be involved in the onset and progression of other systemic diseases, such as diabetes, as well as atherosclerosis, which leads to ischemic heart and brain diseases. Proinflammatory cytokines produced in the inflamed gingivae locally and bacteremia induced through the periodontal ulcerative lesions have been suggested to connect the local and systemic disorders. Periodontal diseases can be controlled by adequate risk management and oral health promotion, and this, in turn, contributes to risk reduction for other life-threatening systemic diseases.
(キーワード): 歯周炎 (periodontal disease) / ライフスタイル (lifestyle) / inflammatory cytokines / cardiovascular disease / 糖尿病 (diabetes mellitus)
(徳島大学機関リポジトリ): ● Metadata: 110288
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050845762395224320

(徳島大学機関リポジトリ: 110288, CiNii: 1050845762395224320) 伊藤博夫 :
歯科治療と感染性心内膜炎の予防,
四国歯学誌, Vol.20, No.2, 191-198, 2008年.

(徳島大学機関リポジトリ): ● Metadata: 109762

(徳島大学機関リポジトリ: 109762) Hiro-O Ito :
Infective endocarditis and dental procedures: evidence, pathogenesis, and prevention,
The Journal of Medical Investigation : JMI, Vol.53, No.3-4, 189-198, Aug. 2006.

(要約): Infective endocarditis is a serious infection occurring on the endothelial surfaces of the heart, especially at the valves. Oral commensal bacteria are the important etiologic agents in this disease. Common dental procedures, even non-surgical dental procedures, can often cause bacteremia of oral commensals. Periodontally diseased patients are at risk from bacteremia even after brushing the teeth. Bacteremia itself rarely affect healthy people but they can result in mortal infective endocarditis in those who have a predisposed risk for this disease, such as those with heart valve diseases, pacemaker implantation, etc. Infective endocarditis is thus established when all the 3 conditions are present simultaneously, i. e., 1) a predisposing impairments in the heart, 2) the introduction of bacteria into the bloodstream, and 3) the virulence of bacteria. Antibiotics have to be adequately used to prevent this infection, however, their frequent uses generates drug-resistant mutant bacteria, which is a serious social problem. The development of novel alternative drugs to be used instead of the current antibiotics is thus highly desired. We are now using several types of combinatorial peptide libraries to search for small size molecular mimetics that can interfere with the adhesion of bacteria to the target organ. The use of such peptides is expected to lead to the development of compounds for a novel preventive drug which does not kill bacteria, thus making it safer and less likely to generate drug-resistant mutants.
(キーワード): Anti-Bacterial Agents / Bacteremia / Dental Care / Dentistry / Endocarditis, Bacterial / Humans / Mouth Diseases / Periodontal Diseases / Risk Factors
(徳島大学機関リポジトリ): ● Metadata: 110807
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.53.189
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16953053; ● Search Scopus @ Elsevier (PMID): 16953053; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.53.189

(徳島大学機関リポジトリ: 110807, DOI: 10.2152/jmi.53.189, PubMed: 16953053) 伊藤博夫 :
牛乳とむし歯 (う蝕) 予防の関係(2), --- 感染症としてのう蝕に対する受動ワクチン ---,
Milk Science, Vol.51, No.3, 2002年. 伊藤博夫 :
牛乳とむし歯 (う蝕) 予防の関係(1), --- カゼインホスホペプチドのう蝕抑制作用 ---,
Milk Science, Vol.51, No.2, 2002年. 伊藤博夫 :
免疫系の抗原認識と特異性,
鹿児島大学歯学部紀要, Vol.19, 23-33, 1999年3月. 北田勝浩, 伊藤博夫, 井上昌一 :
臨床現場でのう蝕予防の実践, --- リスク評価と予防の実際 ---,
歯科医療, Vol.13, No.3, 5-19, 1999年. 井本泰治, 植田正, 伊藤博夫, 古賀敏生 :
抗原のポリエチレングリコール修飾によるトレランス誘導,
臨床免疫, Vol.27, No.9, 1124-1128, 1995年9月.

(キーワード): Antigen / Polyethyleneglycol / Tolerance

伊藤博夫 :
免疫学からみた歯周病, --- 歯周病患者のT細胞機能 ---,
歯科ジャーナル, Vol.31, No.6, 806-815, 1990年6月.

(キーワード): gingivitis / periodontitis / T-cell / MHC class 2

講演・発表

Makoto Fukui, Kaname Miki, Kitamura Masayasu, Hayashida Hideaki, Kawasaki Koji, Rita Cristina Orihuela Campos, Naofumi Tamaki, Maeda Takahiro, Saito Toshiyuki and Hiro-O Ito :
Porphyromonas gingivalis infection may affect anti-atheroschlerotic antibody to phosphorylcholine.,
The 12th International Conference of Asia Academy of Preventive Dentistry, May 2016. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito :
Balance is the key: resveratrol revert oxidative stress conditions in gingival fibroblasts,
第93回IADR, Mar. 2015. Naofumi Tamaki, Makoto Fukui, Hiro-O Ito and Kokeguchi Susumu :
Salivary Antioxidant Activity, Cytokines and Periodontitis: The Nagasaki Island Study,
International Association for Dental Research-Asia Pacific Region(IADR-APR), Bangkok, Aug. 2013. Naofumi Tamaki and Hiro-O Ito :
Relationship between plasma reactive oxygen species and progression of periodontitis,
ICAAPD, ウランバートル, Feb. 2013. Yoshihisa Yamashita and Hiro-O Ito :
Structural shift of microbiota in dental plaque formed on a hydroxyapatite disk surfaces in vivo.,
6th ASM Conference on Biofilms, マイアミシティー, Sep. 2012. Hiro-O Ito :
Periodontal disease as a risk factor for several systemic lifestylerelated diseases. in Symposium 4: Addressing concerns in other oral conditions.,
ICAAPD, ウランバートル, Sep. 2012. Makoto Fukui, Kaname Miki, Naofumi Tamaki and Hiro-O Ito :
Relationship between atherosclerosis and anti-phosphorylcholine antibodies in saliva and plasma,
ICAAPD, ウランバートル, Sep. 2012. Naofumi Tamaki and Hiro-O Ito :
Relationship between plasma reactive oxygen species and progression of periodontitis,
ICAAPD, ウランバートル, Sep. 2012. Naofumi Tamaki, Makoto Fukui and Hiro-O Ito :
Levels of Serum Markers of Oxidative Stress and Antibodies against Periodontal Pathogens in Community-dwelling Adults with and without Periodontitis: Goto Study,
International Conference on Porphyromonas gingivalis and Related Bacterial Species, Nagasaki, Aug. 2012. Makoto Fukui, M. Mani, K. Uno, Kosuke Kataoka and Hiro-O Ito :
Cytokine profile in human saliva and its relationship with that in blood.,
9th International Conference Asian Academy of Preventive Dentistry, Kuala Lumpur, Malaysia, Nov. 2010. Kosuke Kataoka, Ke. Fujihashi, R. Kobayashi, S. R. Gilbert, S. Sekine, Makoto Fukui, Y. Fukuyama, S. Kawabata, Hiro-O Ito and Ko. Fujihashi :
Nasal Cholera Toxin Activated Mucosal Densritic Cells, but not CD4+ Cells Elicits TI-IgA Class Switching Recombination by B-1 B Cells.,
14th International Congress of Immunology, Aug. 2010. T. Baatarjav, Kosuke Kataoka, Makoto Fukui, A. S. Mulyatno, K. Fujihashi and Hiro-O Ito :
Nasal Vaccination with Phosphorylcholine plus Flt3 Ligand Gene as a Mucosal Adjuvant Inhibits Streptococcus pneumoniae Colonization.,
14th International Congress of Immunology, Kobe, Aug. 2010. Makoto Fukui, Daisuke Hinode, Masaaki Yokoyama, Masami Yoshioka and Hiro-O Ito :
Salivary Stress Makers in Patients with Complaint of Halitosis.,
7th Congress of Asian Academy of Preventive Dentistry, Okayama, Nov. 2006. Hiro-O Ito, M Fukui and al et :
Salivary stress markers in patients with complaint of halitosis.,
7th Congress of Asian Academy of Preventive Dentistry, Okayama, Nov. 2006. Hiro-O Ito :
Prevention of infective endocarditis in dental procedures and combinatorial libraries,
The 2nd HiPep Okinawa International Workshop (Uruma, 沖縄バイオテクノロジー研究開発センター), Uruma, Okinawa, Jun. 2006. H Okada, Y Shimabukuro, Y Kassai, Hiro-O Ito, Takashi Matsuo, S Ebisu and Y Harada :
The Function of Gingival Lymphocytes on the Establishment of Human Periodontitis,
Advances in Dental Research, Vol.2, No.2, 364-367, Kobe, Nov. 1988. 長田卓也, 宮生竜生, 玉木直文, 三木かなめ, 伊藤博夫 :
MA-Tを用いた洗口が口臭と口腔細菌に及ぼす効果,
第61回四国歯学会, 2023年3月. 玉木直文, 三木かなめ, 伊藤博夫 :
ヒト歯肉上皮細胞における抗癌剤による有害事象へのレスベラトロールの効果,
日本口腔衛生学会, 2022年5月. 神田和弘, 福永隼也, 玉木直文, 三木かなめ, 伊藤博夫 :
CHX洗口液の口臭に対する効果,
四国歯学会雑誌, 2022年3月. 木村年秀, 丸岡三沙, 小松明日香, 皆本麻結, 玉木直文, 伊藤博夫, 渡邊達夫 :
歯肉を叩く電動歯ブラシ・TAPG®で歯肉出血の改善を試みた2症例の報告,
第32回近畿・中国・四国日本口腔衛生学会・総会, 2021年10月. 三木かなめ, 陳舒, 玉木直文, 伊藤博夫 :
ヒト歯肉線維芽細胞における天然ハーブ・キャッツクロー抽出物の抗炎症効果,
第32回近畿・中国・四国日本口腔衛生学会・総会, 2021年10月. 三木かなめ, 陳舒, 玉木直文, 伊藤博夫 :
天然ハーブ・キャッツクローのP. gingivalis LPS 誘発性サイトカイン発現に対する抑制作用,
第24回日本歯科医学会学術大会, 2021年9月. 玉木直文, 陳舒, 三木かなめ, 伊藤博夫 :
口腔粘膜炎のin vitro モデルにおけるレスベラトロールの抗酸化・抗炎症効果,
第70回日本口腔衛生学会総会, 2021年5月. 王思淼, 渡辺朱理, 松山美和, 玉木直文, 福井誠, 三木かなめ, 伊藤博夫 :
ATP測定法による口腔内細菌数の評価,
第30回近畿・中国・四国口腔衛生学会総会, 2019年9月.

(キーワード): ATP測定 / 口腔内細菌

大毛健一郎, 野上幸裕, 玉木直文, 三木かなめ, 伊藤博夫 :
口腔内清掃による口臭値と口腔内細菌数指標の変化,
第55回四国歯学会, 2019年9月. Chen Shu, Naofumi Tamaki, Kaname Miki and Hiro-O Ito :
Preventive effects of resveratrol against 5-fluorouracil induced cytotoxicity in HaCaT cells,
第68回日本口腔衛生学会・総会, May 2019. 伊藤博夫 :
唾液を用いた口腔の健康診査 :, --- 唾液からわかること，現状と将来展望 ---,
第62回中国四国合同産業衛生学会産業歯科保健部会研修会, 2018年11月. 三木かなめ, 福井誠, 陳舒, 玉木直文, 伊藤博夫 :
天然ハーブ・キャッツクロー抽出物の歯周病原菌に対する抗菌効果,
第29回近畿中国四国口腔衛生学会, 2018年9月. 高野栄之, 宮本由貴, 福井誠, 玉木直文, 伊藤博夫, 東雅之 :
当院栄養サポートチームにおける歯科医師連携の現況,
四国歯学会第53回例会, 2018年7月. 高野栄之, 小野信二, 福井誠, 玉木直文, 吉岡昌美, 桃田幸弘, 伊藤博夫, 東雅之 :
徳島大学病院NSTにおける歯科医師連携の現況と課題,
第15回日本口腔ケア学会総会・学術大会, 2018年4月. 福井誠, 玉木直文, 福田功, 伊藤博夫 :
多項目唾液バイオマーカー検査による歯周組織炎症の評価,
日本歯周病学会60周年記念京都大会, 2017年12月. 福井誠, 陳舒, 三木かなめ, 玉木直文, 福田功, 伊藤博夫 :
歯肉炎症の評価における3種の唾液潜血検査の有用性の検討第28回近畿・中国・四国口腔衛生学会総会 2017年10月1日広島大学歯学部(広島市),
第28回近畿・中国・四国口腔衛生学会総会, 2017年10月. 伊藤博夫 :
歯周病唾液検査の現状と将来展望第66回日本口腔衛生学会総会シンポジウム7「一次予防を科学する基礎研究の新展開」(山形テルサ), 2017年5月31日∼6月3日,
口腔衛生学会雑誌, Vol.67, No.Suppl, 88-91, 2017年5月. 玉木直文, オリウエラカンポスリタクリスティーナ, 陳舒, 福井誠, 伊藤博夫 :
実験的口腔粘膜炎モデルにおけるレスベラトロールの効果の検討,
第66回日本口腔衛生学会・総会, 2017年5月. 楢崎史也, 松岡志織, 玉木直文, 福井誠, 伊藤博夫 :
フッ化物洗口の口腔環境に及ぼす効果,
四国歯学会第50回例会, 2017年3月. 松尾亮水野明彦鈴木雅春鈴木章一郎柳田國雄, 玉木直文, 福井誠, 伊藤博夫 :
糖尿病外来患者におおけるHbA1c値の2年間の経過とかかりつけ歯科医の関係,
第51回日本成人病(生活習慣病)学会, 2017年1月. 福井誠, 早渕純子, 西尾よしみ, 竹内早苗, 横山小百合, 井﨑ゆみ子, 伊藤博夫, 前田健一 :
歯科検診10年の取り組み,
2016年10月. 福井誠, 三木かなめ, オリウエラカンポスリタクリスティーナ, 玉木直文, 伊藤博夫 :
唾液潜血検査の判定を妨害する因子の検索,
第27回近畿・中国・四国口腔衛生学会・総会, 2016年10月. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui and Hiro-O Ito :
Polyphenols reduce collateral damage in HGFs induced by oxidative stress,
44th Annual Meeting of the Japanese Society for Immunology, Nov. 2015. Makoto Fukui, Rita Cristina Orihuela Campos, Naofumi Tamaki and Hiro-O Ito :
Influence of infection with Porphyromonas gingivalis on relationship between atherosclerosis and antiphosphorylcholine antibody,
44th Annual Meeting of the Japanese Society for Immunology, Nov. 2015. Makoto Fukui, Kaname Miki, K Kitamura, H Hayashida, K Kawasaki, Rita Cristina Orihuela Campos, Naofumi Tamaki, T Maeda, T Saito and Hiro-O Ito :
Atherosclerosis-preventive activity of anti-phosphorylcholine antibody may be interfered by infection with Porphyromonas gingivalis : The Nagasaki Islands study,
63rd Annual Meeting of the Japanese Association of Dental Research, Oct. 2015. 岡田寿郎, 福井隆夫, 藤村英二, 大月陽子, 西雅子, 豊島健治, 福井誠, 玉木直文, 伊藤博夫, 奥弘文 :
日本歯科医師会の``生活支援プログラム''による健診事業の参加者の満足度および行動変容への効果,
第26回近畿・中国・四国口腔衛生学会総会, 2015年9月. 鈴木雅博, 水野昭彦, 松尾亮, 玉木直文, 福井誠, 伊藤博夫 :
糖尿病教育入院患者における歯肉溝液中の炎症関連バイオマーカーの経時的変化,
第26回近畿・中国・四国口腔衛生学会総会, 2015年9月. 橋本智惠子, 西窪美波, 玉木直文, 福井誠, 伊藤博夫 :
キシリトールガム咀嚼による唾液分泌能と唾液細菌叢の変化,
第45回四国歯学会, 2015年6月. 水野昭彦, 松尾亮, 鈴木雅博, 玉木直文, 福井誠, 三木かなめ, 松瀬亮一, 伊藤博夫 :
市民検診と糖尿病患者データにおける歯肉溝浸出液バイオマーカーの比較検討,
第64回口腔衛生学会総会, 2015年5月. 玉木直文, オリウエラカンポスリタクリスティーナ, 福井誠, 三木かなめ, 伊藤博夫 :
水素水の抗酸化・抗炎症作用の検討,
第64回口腔衛生学会総会, 2015年5月. 福井誠, 岡田寿郎, 三木かなめ, 玉木直文, 伊藤博夫 :
``標準的な成人歯科健診プログラム・保健指導マニュアル''に基づいた歯科保健事業の効果について,
第64回口腔衛生学会総会, 2015年5月. 松尾亮, 水野昭彦, 鈴木雅博, 玉木直文, 福井誠, 三木かなめ, 伊藤博夫 :
歯肉溝浸出液バイオマーカーと血液データの比較検討,
第49回日本成人病(生活習慣病)学会, 2015年1月. Makoto Fukui, Kaname Miki, Rita Cristina Orihuela Campos, Naofumi Tamaki and Hiro-O Ito :
Levels of various cytokines in human saliva from generally healthy individuals.,
62nd Annual Meeting of the Japanese Association for Dental Research, Dec. 2014. 福井隆夫, 小林弘茂, 渋谷敦人, 岡田寿朗, 豊嶋健, 福井誠, 玉木直文, 伊藤博夫, 奥弘文 :
日歯生活歯援プログラムに基づく平成25年度成人歯科健診事業実施報告,
第25回近畿・中国・四国口腔衛生学会総会, 2014年10月. 高尾健二郎, 新居麻美子, 福井誠, 三木かなめ, オリウエラカンポスリタクリスティーナ, 玉木直文, 伊藤博夫 :
歯肉炎症の客観的・定量的診断を目指した市販免疫クロマトグラフィー唾液潜血テストの改良,
第25回近畿・中国・四国口腔衛生学会総会, 2014年10月. 水野昭彦, 松尾亮, 玉木直文, 福井誠, 三木かなめ, 伊藤博夫 :
歯肉溝浸出液バイオマーカーと血液データの比較,
第25回近畿・中国・四国口腔衛生学会総会, 2014年10月. 玉木直文, 水野昭彦, 松尾亮, 正木文浩, 中川徹, 鈴木雅博, 青木良平, 松瀬亮一, 福井誠, 伊藤博夫 :
歯肉溝浸出液中バイオマーカーと腎機能・糖尿病指標との関連,
第7回日本口腔検査学会総会・学術大会, 2014年9月. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito :
Effects of three different antioxidants on human gingival fibroblasts exposed to oxidative stress,
2014 Tokushima Bioscience retreat, Sep. 2014. 福井誠, 三木かなめ, オリウエラカンポスリタクリスティーナ, 玉木直文, 伊藤博夫 :
健常者における唾液中サイトカインプロファイルの解析,
第63回口腔衛生学会総会, 2014年5月. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito :
Effects of three antioxidant compounds on human gingival fibroblasts under oxidative stress,
第63回口腔衛生学会総会, May 2014. 玉木直文, オリウエラカンポスリタクリスティーナ, 福井誠, 伊藤博夫 :
口蓋創傷治癒モデルにおける水素水摂取の効果,
第4回分子状水素医学シンポジウム, 2014年2月. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito :
Effects of different antioxidants on human gingival fibroblasts under oxidative stress,
第5回心・血管クラスターミニリトリート, Jan. 2014. 小林弘茂, 渋谷敦人, 福井隆夫, 岡田寿朗, 豊嶋健治, 福井誠, 玉木直文, 伊藤博夫, 奥弘文 :
日歯プログラムに基づく成人歯科健診における唾液検査および質問紙調査と歯周状態の関連性について,
第24回近畿・中国・四国口腔衛生学会総会, 2013年10月. 福井誠, 三木かなめ, 玉木直文, 伊藤博夫 :
唾液中サイトカインと口腔健康状態の関連性,
第24回近畿・中国・四国口腔衛生学会総会, 2013年10月. 福井誠, 玉木直文, 伊藤博夫 :
唾液を用いた高齢者の口腔健康状態把握のための基盤的研究,
第56回秋季日本歯周病学会学術大会, 2013年9月. 伊藤博夫 :
公衆衛生学的視点から見た歯肉の健康指標の現状と将来展望. 学会主導企画シンポジウム「歯周病の予防戦略」,
第56回秋季日本歯周病学会, 2013年9月. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito :
Effects of different antioxidants on the biological properties of human gingival fibroblasts response toward ROS,
Tokushima Bioscience retreat, Sep. 2013. 宮城紫, 木村光孝, 井上尚兵, 中尾允泰, 佐野茂樹, 福井誠, 伊藤博夫 :
OPA法を基盤とした新しい口臭検査法の開発,
第11回プロセス化学東四国フォーラムセミナー, 2013年6月. Rita Cristina Orihuela Campos, Naofumi Tamaki, Makoto Fukui, Kaname Miki and Hiro-O Ito :
Real time xCELLigence analysis of antioxidant agents on human gingival fibroblast cells viability,
第62回日本口腔衛生学会総会, May 2013. 福井誠, 三木かなめ, オリウエラカンポスリタクリスティーナ, 玉木直文, 伊藤博夫 :
健常者における唾液中サイトカイン分析,
第62回日本口腔衛生学会・総会, 2013年5月. 宮城紫, 木村光孝, 井上尚兵, 中尾允泰, 佐野茂樹, 福井誠, 伊藤博夫 :
OPA法を基盤とした新しい口臭検査法の開発,
日本薬学会第133年会, 2013年3月. 福井誠, 玉木直文, 三木かなめ, 伊藤博夫 :
動脈硬化リスク評価のための唾液・血液中の抗ホスホリルコリン抗体の測定,
心血管クラスターリトリート, 2013年1月. SA Mulyatno, Naofumi Tamaki, Makoto Fukui and Hiro-O Ito :
Mucosal adjuvanticity of bacterial lipopolysaccharide in compared with cholera toxin,
Proceedings of the Japanese Society for Immunology, Dec. 2012. 岡田寿朗, 小林宏茂, 福井隆夫, 渋谷敦人, 武部裕光, 豊嶋健治, 福井誠, 伊藤博夫, 奥弘文 :
平成22年度に香川県内で実施された成人歯科健診モデル事業実施報告,
第22回日本歯科医学会総会, 2012年11月. 宮城紫, 木村光孝, 井上尚兵, 中尾允泰, 佐野茂樹, 福井誠, 伊藤博夫 :
オルトフタルアルデヒド法を基盤とした新規メチルメルカプタン蛍光標識法,
第51回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2012年11月. 天羽崇, 日野出大輔, 平島美穂, 吉岡昌美, 横山正明, 玉谷香奈子, 福井誠, 伊藤博夫, 片岡宏介 :
舌苔中の歯周病関連細菌と口臭との関連性,
第23回近畿中国四国口腔衛生学会総会, 2012年10月. 福井誠, 玉木直文, 伊藤博夫 :
成人歯科健診事業における唾液検査と歯周所見の一致性について,
第23回近畿中国四国口腔衛生学会総会, 2012年10月. 福井誠, 玉木直文, 伊藤博夫 :
平成23年度に香川県内で実施した「成人歯科健診モデル事業」における保健行動目標の達成状況について,
第23回近畿中国四国口腔衛生学会総会, 2012年10月. 福井誠, 三木かなめ, 玉木直文, 伊藤博夫 :
動脈硬化リスク評価のための唾液・血液中の抗ホスホリルコリン抗体の測定,
第5回日本口腔検査学会総会・学術大会, 2012年8月. 玉木直文, 伊藤博夫 :
歯周病進行と酸化ストレス,
四国歯学会雑誌, 2012年6月. SA Mulyatno, Kosuke Kataoka, Makoto Fukui, Kaname Miki, RC Orihuela-Campos and Hiro-O Ito :
Modulation of Mucosal Immune Response by Bacterial Lipopolysaccharide in Nasal Vaccination Model,
第61回日本口腔衛生学会・総会, May 2012. 福井誠, SA Mulyatno, 後藤優樹, 三木かなめ, RC Orihuela-Campos, 片岡宏介, 林田秀明, 北村雅保, 川崎浩二, 関田孝晴, 中里未央, 前田隆浩, 齋藤俊行, 伊藤博夫 :
ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性-第二報-,
第61回日本口腔衛生学会・総会, 2012年5月. 福井誠, ムルヤトノサプタ, バータルジャフツェルメグ, 後藤優樹, 片岡宏介, 林田秀明, 川崎浩二, 前田隆浩, 中里未央, 齋藤俊行, 伊藤博夫 :
ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性:五島研究,
2011年10月. ムルヤトノサプタ, 片岡宏介, 関根伸一, バータルジャブツェルメグ, 福井誠, 金川裕子, 後藤優樹, 伊藤博夫 :
経鼻粘膜アジュバントCpG ODNはマクロファージのTLR9発現と歯周病細菌抗原特異的Th1免疫応答を誘導する,
2011年10月. 渋谷敦人, 小林弘茂, 福井隆夫, 岡田寿朗, 豊嶋健治, 福井誠, 片岡宏介, 伊藤博夫, 奥弘文 :
平成22年度に香川県内で実施された成人歯科健診モデル事業実施報告,
2011年10月. T. Baatarjav, Kosuke Kataoka, S. Mulyatno, Makoto Fukui, H. Kanagawa and Hiro-O Ito :
Nasal Flt3L gene results in mucosal immune responses to PC through induction of B-1 B cells.,
第59回口腔衛生学会総会, Oct. 2010. 片岡宏介, 関根伸一, バータルジャフツェルメグ, ムルヤトノサプタ, 福井誠, 金川裕子, 伊藤博夫 :
遺伝子改変植物による歯周病原菌定着阻害能を有するヒト唾液タンパク質スタセリンペプチドの発現,
第59回口腔衛生学会総会, 2010年10月. 福井誠, ムルヤトノサプタ, バータルジャフツェルメグ, 片岡宏介, 林田秀明, 川崎浩二, 斎藤俊行, 伊藤博夫 :
ヒト血中および唾液中の抗ホスホリルコリン抗体の存在実態,
第59回口腔衛生学会総会, 2010年10月. 片岡宏介, 関根伸一, 福井誠, 横山正明, 伊藤博夫 :
粘膜アジュバントCpG オリゴヌクレオチドによるPorphyromonas gingivalis 線毛抗原特異的抗体応答の誘導,
第53回日本歯周病学会秋季学術大会, 2010年9月. 片岡宏介, 福井誠, 金川裕子, 伊藤博夫 :
ヒト唾液タンパク質由来ペプチドの歯周病菌定着阻害効果,
第21回近畿・中国・四国口腔衛生学会総会, 2010年6月. 福井誠, サプタムルヤトノ, バータルジャフツェルメグ, 片岡宏介, 伊藤博夫 :
ヒトの唾液中および血中に存在する抗ホスホリルコリン抗体の検出,
第21回近畿・中国・四国口腔衛生学会総会, 2010年6月. 三崎昌和, 渋谷敦人, 岡田寿朗, 阿部直樹, 豊嶋健治, 福井誠, 片岡宏介, 伊藤博夫, 奥弘文 :
平成21年度に香川県内で実施された成人歯科健診モデル事業実施報告,
第21回近畿・中国・四国口腔衛生学会総会, 2010年6月. 片岡宏介, 中垣晴男, 関根伸一, 福井誠, 伊藤博夫, 雫石聰 :
Fusobacterium nucleatum表層タンパク質FomAと唾液高チロシンタンパク質スタセリンペプチドとの相互作用,
第53回日本歯周病学会春季学術大会, 2010年5月. Kosuke Kataoka, Hiro-O Ito, Makoto Fukui, Baatarjav Tselmeg and 藤橋浩太郎 :
Induction of PC-specific T15 idiotype Ab in mucosal secretion by Flt3 ligand plasmid as a mucosal adjuvant,
日本免疫学会総会学術集会記録, Vol.39, 193, Dec. 2009. 森本晃允, 中尾允泰, 佐野茂樹, 伊藤博夫 :
OPA法ならびにエルマン法を基盤とするチオール捕捉標識反応剤の合成開発,
第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2009年11月. 高松夏子, 福井誠, 片岡宏介, 泉福英信, 伊藤博夫 :
歯根面う蝕発生における唾液中抗PAc (361-386) IgA 抗体の役割,
第59回日本口腔衛生学会総会(岐阜市，長良川国際会議場)2009年10月10日口腔衛生学会雑誌, Vol.59, No.4, 367, 2009年10月. バータルジャフツェルメグ, ムルヤトノサプタ, 金川裕子, 片岡宏介, 伊藤博夫, 福井誠 :
唾液タンパク質由来ペプチド複合体による歯周病菌定着阻害能の検討,
第59回日本口腔衛生学会総会(岐阜市，長良川国際会議場) 口腔衛生学会雑誌, Vol.59, No.4, 349, 2009年10月. 岡田寿朗, 三崎昌和, 佐々木浩司, 武部裕光, 山下喜世弘, 福井誠, 伊藤博夫, 奥弘文 :
香川県内で実施された歯科保健指導モデル事業報告,
第20回日本口腔衛生学会近畿·中国·四国地方会 (広島国際会議場), 2009年6月. 金川裕子, 福井誠, 米津隆仁, 横山正秋, 安達聡, ツェルメグ B., 片岡宏介, 伊藤博夫 :
徳島県民の歯科定期歯科健診受診行動に関する心理的要因の調査,
第20回日本口腔衛生学会近畿·中国·四国地方会 (広島国際会議場), 2009年6月. 福井誠, 伊藤博夫 :
フゾバクテリウム反応性唾液sIgA抗体と舌苔との関連性,
第20回日本口腔衛生学会近畿·中国·四国地方会 (広島国際会議場), 2009年6月. 福井誠, 日野出大輔, 横山正明, バータルジャフツェルメグ, 片岡宏介, 伊藤博夫 :
フゾバクテリウム反応性唾液sIgA抗体と舌苔との関連性,
口腔衛生学会雑誌, Vol.59, No.5, 606-607, 2009年6月.

(キーワード): *Secretory IgA

万井正章, 宇野賀津子, 福井誠, 伊藤博夫 :
唾液中サイトカイン測定の健康診査への応用に関する研究,
第74回日本インターフェロン・サイトカイン学会学術集会(京都大学芝蘭会館), 2009年6月. 伊藤博夫, B. ツェルメグ, 片岡宏介, 福井誠 :
肺炎球菌抗原とDNAアジュバント経鼻投与による粘膜免疫応答の解析,
第20回日本口腔衛生学会近畿·中国·四国地方会 (広島国際会議場), 2009年6月. 森本晃允, 佐野茂樹, 長尾善光, 伊藤博夫 :
メタンチオール捕捉標識反応剤の合成開発,
日本薬学会第129年会, 2009年3月. 森本晃允, 佐野茂樹, 長尾善光, 伊藤博夫 :
揮発性チオール捕捉反応剤の合成開発研究,
第47回日本薬学会·日本薬剤師会·日本病院薬剤師会中国四国支部学術大会, 2008年11月. 岡田寿朗, 三崎昌和, 佐々木浩司, 武部裕光, 山下喜世弘, 横山正明, 福井誠, 伊藤博夫, 奥弘文 :
香川県内で実施された成人歯科健診モデル事業報告,
日本歯科医師会雑誌, Vol.61, No.5, 555, 2008年8月. 横山正明, 福井誠, 本那智昭, 髙松夏子, 増田かなめ, 伊藤博夫 :
口腔清潔度の指標としての唾液中総細菌数, --- リアルタイムPCR法によるハイスループット測定 ---,
口腔衛生学会雑誌, Vol.57, No.5, 692, 2007年10月. 岡田寿朗, 三崎昌和, 佐々木浩司, 亀井稔之, 武部裕光, 山下喜世弘, 横山正明, 福井誠, 伊藤博夫, 奥弘文 :
香川県内で実施された成人歯科健診モデル事業報告,
口腔衛生学会雑誌, Vol.57, No.5, 689, 2007年10月. 福井誠, 日野出大輔, 横山正明, 吉岡昌美, 伊藤博夫 :
口臭患者における唾液ストレスマーカーの評価,
口腔衛生学会雑誌, Vol.57, No.4, 482, 2007年8月. 髙松夏子, 増田かなめ, 福井誠, 横山正明, 吉岡昌美, 伊藤博夫 :
歯根面う蝕のリスクファクター調査,
口腔衛生学会雑誌, Vol.57, No.4, 423, 2007年8月. 横山正明, 日野出大輔, 福井誠, 吉岡昌美, 伊藤博夫 :
歯周病原細菌Campylobacter rectusと妊娠期の歯周状態との関連性,
日本歯周病学会会誌, Vol.49, 176, 2007年8月. Masaaki Yokoyama, Daisuke Hinode, Makoto Fukui, Masami Yoshioka and Hiro-O Ito :
Relationship between Campylobacter rectus and periodontal status during pregnancy,
「魅力ある大学院教育」イニシアティブ・第1回国際シンポジウム, Mar. 2007. 吉岡昌美, 本那智昭, 福井誠, 横山正明, 田部慎一, 増田かなめ, 日野出大輔, 伊藤博夫 :
学校歯科健診における歯肉炎の評価についての考察,
口腔衛生学会雑誌, Vol.56, No.5, 723, 2006年10月. 横山正明, 伊藤博夫, 他 :
妊婦における歯周疾患と唾液中の女性ホルモンおよび血管内皮増殖因子(VEGF)の関連性,
第55回日本口腔衛生学会総会, 2006年10月. 吉岡昌美, 伊藤博夫, 他 :
学校歯科健診における歯肉炎の評価についての考察,
第55回日本口腔衛生学会総会, 2006年10月. 伊藤博夫 :
シンポジウム5「口腔保健の免疫学的展開」口腔常在菌のホスホリルコリン抗原決定基に対する免疫応答の誘導と，抗肺炎球菌応答の修飾について,
第55回日本口腔衛生学会総会, 2006年10月. 横山正明, 日野出大輔, 福井誠, 田部慎一, 吉岡昌美, 伊藤博夫 :
妊婦における歯周疾患と唾液中の女性ホルモンおよび血管内皮増殖因子(VEGF)の関連性,
口腔衛生学会雑誌, Vol.56, No.4, 593, 2006年8月. 伊藤博夫 :
歯学・歯科医療におけるサイエンスとエビデンスの融合を求めて,
第30回四国歯学会総会(徳島大学歯学部), 2006年7月. 吉岡昌美, 伊藤博夫, 他 :
吉岡昌美ら，学校歯科健診における歯肉炎の評価についての考察,
第17回日本口腔衛生学会近畿·中国·四国地方会 (和歌山県歯科医師会館), 2006年6月.

研究会・報告書

Chen Shu, Naofumi Tamaki, Kaname Miki and Hiro-O Ito :
Protective effects of resveratrol against 5-FU-induced inflammatory responses in HaCaT cells.,
13, Jan. 2021. Chen Shu, Naofumi Tamaki, Kaname Miki and Hiro-O Ito :
Resveratrol attenuates 5-FU-induced cell damage via activation of Nrf2 antioxidant pathway,
2020 69, Apr. 2020. Chen Shu, Naofumi Tamaki, Kaname Miki and Hiro-O Ito :
Resveratrol attenuates 5-FU-induced cytotoxic reaction by inhibiting NF-kB and promoting f Nrf2 translocation to the nucleus in the HaCaT cell model,
2019 Tokushima Bioscience Retreat, Sep. 2019. 玉木直文, 陳舒, 工藤保誠, 三木かなめ, 石丸直澄, 伊藤博夫 :
実験的口腔粘膜モデルにおけるレスベラトロールの抗酸化・抗炎症効果,
第38回分子病理研究会, 2019年7月. 伊藤博夫 :
徳島県立総合大学校オンリーワン徳島学講座「むし歯と歯周病の早期発見と予防，そして成人病(生活習慣病)に罹りにくい全身の健康づくりへ徳島県立総合教育センター(徳島県板野郡板野町)特別講義,
徳島県立総合大学校特別講義, 2017年9月. 伊藤博夫 :
歯周病の予防と治療:健康の地力を高めるために必要なこと平成28年度歯周疾患予防管理研修・糖尿病の医科歯科連携研修会 (一社)島根県歯科医師会，島根県健康福祉部健康増進課，島根県歯科医師会館(島根県松江市)特別講演,
島根県歯科医師会特別講演, 2017年1月. 福井誠, 早渕純子, 西尾よしみ, 武内早苗, 横山小百合, 井﨑ゆみ子, 伊藤博夫, 前田健一 :
歯科検診10年の取り組み,
全国大学保健管理研究集会プログラム・抄録集, 51, 2016年9月.

(キーワード): *口腔保健

日本口腔衛生学会禁煙推進委員会, 埴岡隆, ほか22名, 伊藤博夫 :
タバコ使用と口腔微生物の関係, --- 1. 歯の周囲およびインプラント周囲の細菌 ---,
日本口腔衛生学会誌, Vol.72, 185-189, 2022年. 山下喜久, 天野敦雄, 伊藤博夫, 友藤孝明, 久保庭雅恵 :
口と全身の疾患と健康を評価する唾液検査法の開発, --- (平成29年度採択プロジェクト研究報告) ---,
日本歯科医学会雑誌, Vol.39, 38-43, 2020年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1010572092265505280

(CiNii: 1010572092265505280)

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 液性検体含有メルカプタンの定量分析法開発への基礎的研究―口臭の疫学調査へ向けて (研究課題/領域番号: 21K10256 )
疫学研究に適用可能な，唾液の液体分析法による多検体自動処理の口臭検査法の開発 (研究課題/領域番号: 18K09912 )
歯牙喪失における遺伝子環境交互作用に関する研究：ゲノムワイド関連解析を用いた検討 (研究課題/領域番号: 18K09908 )
天然ハーブ・キャッツクローの抗酸化作用と歯周病予防に関する基礎的研究 (研究課題/領域番号: 16K11859 )
生体反応の化学的測定に基づく客観的な新規歯周病診断システムの開発 (研究課題/領域番号: 26293442 )
ＥＳＲ法による活性酸素種測定と歯周病との相互連関の解明および抗酸化物の効果 (研究課題/領域番号: 24593155 )
ヒト唾液中に含まれるサイトカインの正常範囲の決定 (研究課題/領域番号: 23593091 )
新規ハイスループット口臭検査法の開発を目指した基礎的研究 (研究課題/領域番号: 22592336 )
天然ペプチドライブラリーからのカンジダ菌定着抑制物質の検索 (研究課題/領域番号: 19592406 )
胸腺非依存性抗原特異的唾液IgA抗体誘導のための自然免疫賦活化メカニズムの解明 (研究課題/領域番号: 19592403 )
デザインペプチドライブラリー構築による細菌付着阻止分子探索と感染性心内膜炎予防 (研究課題/領域番号: 18390569 )
感染症局所病変の病型決定に関与する宿主遺伝子の探索 (研究課題/領域番号: 17659593 )
細菌の組織定着抑止機序と分子デザインによる感染症予防の研究 (研究課題/領域番号: 16390540 )
歯垢による感染性心内膜炎発症の実験的検証 (研究課題/領域番号: 15592211 )
集団成人健康診断における採血サンプルの歯周疾患診断への応用 (研究課題/領域番号: 12672037 )
抗原エピトープ高次構造に基づく新奇な抗歯周病合成ワクチンの開発 (研究課題/領域番号: 12557188 )
コンビナトリアルライブラリーを用いた感染性心内膜炎予防のためのリード化合物探索 (研究課題/領域番号: 12557187 )
口腔レンサ球菌のフィブロネクチン受容体遺伝子の解析と発現タンパクの臨床応用 (研究課題/領域番号: 12470464 )
フィブロネクチン分子の細菌体結合エピトープの構造解析 (研究課題/領域番号: 11671873 )
Streptococcus milleriの生体付着機構解析ーとくにlamininを介した付着機構についてー (研究課題/領域番号: 09672112 )
咀嚼機能が免疫機能に及ぼす影響 -高齢者について- (研究課題/領域番号: 09671994 )
歯周炎に伴う免疫反応の特異性の解明とその制御 (研究課題/領域番号: 09671924 )
タンパク質の高次構造に依存する抗原エピトープ同定法の開発 (研究課題/領域番号: 08877282 )
抗原と抗体の特異的結合に関与する立体構造の同定 (研究課題/領域番号: 07857135 )
細菌内毒素とリゾチームの相互作用 (研究課題/領域番号: 07457448 )
歯周病関連細菌のリポ多糖がは破骨細胞の分化ならびに活性化に及ぼす影響 (研究課題/領域番号: 06771669 )
歯周炎患者の免疫応答の特異性(歯周病原性細菌特異的反応と自己交叉反応の相関について) (研究課題/領域番号: 05671595 )
骨芽細胞ならびに破骨細胞における情報伝達機構 (研究課題/領域番号: 05454511 )
歯周炎患者の体液性免疫応答の特異性(Porphgromonas gingivalisの主要抗原の同定・特に多糖抗原に関して) (研究課題/領域番号: 04671157 )
多変量解析による歯周炎ハイリスク患者の診断 (研究課題/領域番号: 04454471 )
歯周炎における免疫制御機構(歯周炎の病因に関与するT細胞の自己認識能の検討) (研究課題/領域番号: 03404056 )
歯周疾患における線維芽細胞と免疫担当細胞間相互作用に関する研究 (研究課題/領域番号: 01571042 )
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研究者総覧で最新データを確認する

2024年4月19日更新

専門分野・研究分野: 予防歯学 (Preventive Dentistry)
所属学会・所属協会: 日本口腔衛生学会
歯科基礎医学会
日本免疫学会
日本歯周病学会
日本公衆衛生学会
International Association for Dental Research
American Society for Microbiology
近畿中国四国口腔衛生学会常任幹事
委員歴・役員歴: 日本口腔衛生学会 (常任理事・会計)
受賞: 2007年1月, 康楽賞 (三木産業株式会社.財団法人三木康楽会)
活動: 香川県歯科医師会健診プログラム検討委員会
上板町「健康かみいた21」策定作業部会
徳島県保健推進センター・徳島保健所事業主セミナー「歯とお口の健康からみた生活習慣病」
徳島大学大学開放実践センター公開講座平成19年度秋冬講座「自分で守る歯と口の健康」(5回シリーズ)
徳島大学大学開放実践センター公開講座平成22年度春夏講座「高齢社会と歯と口と健康」(6回シリーズ)
サルビア会(阿南)講演「脳梗塞・動脈硬化と歯周疾患」2012年3月8日
九州大学大学院セミナー「ホスホリルコリン抗原と心血管疾患，および歯周疾患」2011年10月14日
徳島大学大学開放実践センター公開講座平成23年度秋冬講座「いつまでもお口で食べて健やかに!」
フューチャーセンターセッション「対話の手法でデザインする口の健康と地域の未来」歯学部口腔科学フロンティア推進室:口腔健康科学創成・栄養連携部門
(2006年5月〜2007年3月)
日本口腔衛生学会常任理事

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Jグローバル最終確認日: 2024/4/20 01:17
氏名（漢字）: 伊藤博夫
氏名（フリガナ）: イトウヒロオ
氏名（英字）: ITO Hiro-O
所属機関: 徳島大学教授

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researchmap最終確認日: 2024/4/14 01:21
氏名（漢字）: 伊藤博夫
氏名（フリガナ）: イトウヒロオ
氏名（英字）: ITO Hiro-O
プロフィール: 研究の大目標は「歯と口の健康を通じての、人々の全身の健康とQOL（Quality of Life: 生活の質）への貢献」です。
歯周病や齲蝕症（むし歯）に代表される口腔内に生息する微生物による歯科疾患の予防を目指した、その発生機序についての研究に免疫学・分子生物学的手法を用いて取組んでいます。病院に訪れる患者さんだけではなく、健康な人々を含んだ地域に生活する全ての人々を対象として、歯と口の健康状態と全身の健康状態ならびにQOLとの関連についての疫学的研究にも取組んでいます。
歯学部の学生教育においては、上記の目標達成に貢献する高度な知識・技能・思想を持った歯科医師の育成を目指して、歯学科学生の予防歯科学（口腔衛生学）、衛生公衆衛生学の全部、および社会歯科学の一部を担当しています。大学院学生教育においては、予防歯学、予防歯学演習、実践口腔科学実習、顎口腔発育・社会歯科学実験実習などを担当しています。留学生もたくさん受け入れています。
登録日時: 2010/7/28 00:00
更新日時: 2022/9/8 13:12
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所属ID: 0344020000
所属: 徳島大学
部署: 大学院ヘルスバイオサイエンス研究部予防歯学分野
職名: 教授
学位: 歯学博士
学位授与機関: 大阪大学
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ＫＡＫＥＮで最新データを確認する

2024年4月20日更新

研究者番号: 40213079

所属（現在）: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 教授

所属（過去の研究課題 情報に基づく）*注記: 2018/4/1 – 2022/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 教授
2016/4/1 – 2017/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学系), 教授
2015/4/1 – 2016/4/1 : 徳島大学, 大学院医歯薬学研究部, 教授
2014/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2011/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 教授
2010/4/1 – 2012/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2005/4/1 – 2008/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2005/4/1 – 2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2003/4/1 – 2004/4/1 : 鹿児島大学, 大学院・医歯学総合研究科, 助教授
1998/4/1 – 2002/4/1 : 鹿児島大学, 歯学部, 助教授
1993/4/1 – 1997/4/1 : 九州大学, 歯学部, 助手
1991/4/1 – 1992/4/1 : 大阪大学, 歯学部, 助手
1989/4/1 : 大阪大学, 歯学部, 助手

審査区分/研究分野

研究代表者

医学 / 歯学 / 保存治療系歯学
医学 / 歯学 / 病態科学系歯学(含放射線系歯学)
生物系 / 医歯薬学 / 歯学 / 病態科学系歯学・歯科放射線学
生物系 / 医歯薬学 / 歯学 / 社会系歯学
医学 / 歯学 / 矯正・小児・社会系歯学
小区分57080:社会系歯学関連

研究代表者以外

医学 / 歯学 / 保存治療系歯学
生物系 / 医歯薬学 / 歯学 / 社会系歯学
医学 / 歯学 / 病態科学系歯学(含放射線系歯学)
医学 / 歯学 / 補綴理工系歯学
医学 / 歯学 / 矯正・小児・社会系歯学
医学 / 歯学 / 歯周治療系歯学
小区分57080:社会系歯学関連

キーワード

研究代表者

歯周炎 / IgGサブクラス / Porphyromonas gingivalis / 抗体 / 多糖抗原 / イディオタイプ / モノクローナル抗体 / 熱ショック蛋白質(hsp) / 自己抗原 / 外来抗原 / 歯周病原性細菌 / リポ多糖(LPS) / 内毒素 / 骨破壊 / 骨吸収 / 破骨細胞 / 抗体イディオタイプ抗体 / 単鎖Fv抗体 / 高次構造 / ll型コラーゲン / 核磁気共鳴(NMR) / X線結晶構造解析 / 巻戻し / タンパク質の高次構造 / 抗イディオタイプ抗体 / リコンビナント単鎖Fv抗体 / エピトープ / II型コラーゲン / ホモロジーモデリング / タンパク質の結晶化 / 炎症 / 感染症 / 口腔細菌 / ビリダンスレンサ球菌 / DNAマイクロアレイ / トランスクリプトーム / リアルタイムPCR / RT-PCR / 予防歯科学 / ペプチド / ライブラリー / 定着抑制 / fibronectin / phosphoiylchaline / レンサ球菌 / ペプチドライブラリー / フィブロネクチン / phosphorylcholine / スクリーニング / バイオアッセイ / オンビーズライブラリー / オンビースライブラリー / 感染性心内膜炎 / 口臭検査 / 歯周病 / 有機化学 / エルマン試薬 / チオール捕捉反応 / ハイスループット処理 / メチルメルカプタン / モノマー／エキシマースイッチング / 歯周予防学 / 唾液検査 / 歯科口腔保健 / 歯肉炎 / リゾチーム(ムラミダーゼ) / 細菌リポ多糖(細菌内毒素) / リピドA / 歯周炎(歯周病) / 免疫薬理 / 腫瘍壊死因子 / マイトゲン / 多クローン性B細胞活性化 / Lysozyme (Muramidase) / Lipopolysaccharide (Endotoxin) / Lipid A / Periodontitis (Periodontal Disease) / Osteoclasts / Immunopharmacology / 線毛 / B細胞エピトープ / phage-displayed peptide library / Immunodominance / タンパク質 / 立体構造 / 変性 / 未変性 / fimbriae / monoclonal antibodies / B cell epitopes / higher order structure of proteins / periodontal diseases / 細胞外基質タンパク質 / エピトープ・マッピング / 口腔内細菌 / 常在細菌 / 細胞外基質タンパク / Fibronectin / Extracellular Matrix Protein / Infective Endocarditis / Monoclonal Antibodies / Epitope Mapping / Viridans Streptococci / Oral Indioenous Bacteria / 成人性歯周炎 / Porphyromonoas gingivalis / 免疫 / ワクチン / ファージ・ディスプレイ・ペプチドライブラリー / ファージディスプレイ・ペプチドライブラリー / ファージ・ディスプレイ・ペプチドライプラリー / Periodontal diseases / Adult periodontitis / Vaccine / Fimbriae / Immunity / Phage-displayed peptide library / デコンボリューション / ポジションスキャン / 天然物質ライブラリー / デコンボルーション / Peptide Libraries / Decombolution / Oral Bacteria / Positional Scanning / Natural Product Libraries / 予防歯学 / 口臭 / ハイスループット分析 / オルトフタルアルデヒド（OPA) / メチルメルカプタン（メタンチオール） / 液体クロマトグラフィー / オルトフタルアルデヒド（OPA） / メタンチオール（メチルメルカプタン） / 機器分析 / メチルメルカプタン(メタンチオール) / チオール

研究代表者以外

線維芽細胞 / HLAーDR抗原 / インタ-フェロンガンマ / 酵素抗体法 / 予防歯学 / ワクチン / アジュバント / 粘膜免疫 / 自然免疫 / 唾液IgA抗体 / 経鼻免疫 / クラススイッチング / T細胞非依存性抗原 / 樹状細胞 / B細胞 / IL-5 / 唾液IgA / 口腔衛生 / カンジダ症 / 真菌 / 培養法 / リアルタイムPCR法 / 天然ペプチドライデラリー / 付着アッセイ / フィブロネクチン / 歯周病 / 予防ワクチン / 唾液タンパク質 / 分泌型IgA抗体 / 定着阻害 / 唾液 / サイトカイン / 健常者 / 健康診断 / 酸化ストレス / 抗酸化力 / 歯周疾患 / T細胞 / 自己リンパ球混合培養反応 / CD45R / 細胞接着分子 / 歯肉線維芽細胞 / CD44 / VLA-4 / T細胞クローン / インテグリン / 自己反応性T細胞 / 歯周炎 / 多クロ-ン性B細胞活性化 / periodontal disease / T cells / Autologous mixed lymphocyte reaction / cell adhesion molecule / gingival fibroblast / VLA integrin / ハイリスク患者 / 多変量解析 / 診断法 / 宿主免疫応答 / 歯周ポケット細菌叢 / 歯肉溝浸出液 / 炎症メディエーター / 歯周ポケット内細菌叢 / Periodontitis / High risk patient / Martivariate statistical analysis / Diagnosis / Host immune response / Periodotal pocket microorganisms / Gingival crevicular fluid / Inflammatory mediator / 破骨細胞 / 骨芽細胞 / 骨髄培養系 / マウス間質細胞株 / 細菌性リポ多糖 / デキサメサゾン / 情報伝達 / NF-κB / 骨髄細胞培養系 / NF-kB / 破骨細胞形成 / Osteoclast / Bone marrow / Lipopolysaccharide / Dexamethasone / Basic fibroblast growth factor / IL-1 / M-CSF / NF-kappaB / 高齢者 / 咀嚼機能 / 免疫学的検査 / 義歯 / ADL / elderly / masticatory function / immunological test / denture / ADL (activity of daily living) / Streptococcus milleri / laminin / enolase / 細胞外基質 / 組織付着(定着) / 遺伝子クローニング / PCR法 / 付着 / 定着 / Streptococcus milleri群 / Streptococcus anginosus / Streptococcus constellatus / Streptococcus intermedius / 細胞接着 / 細胞外マトリックス / extracellular matrix / tissue adhesion / gene cloning / polymerase chain reaction (PCR) / Nutritionally variant streptococci (NVS) / Granulicatella adiacens / 口腔細菌 / 感染性心内膜炎 / 2',3'-cyclic nucleotide 2'-phosphodiesterase / Polymerase chain reaction (PCR) / 細胞外基質タンパク質 / DNAライブラリー / 発現ライブラリー / 蛍光標識 / Oral indigenous bacteria / Infective endocarditis / Fibronectin / Gene cloning / 2', 3' -cyclic nucleotide 2' -phosphodiesterase / ペプチドライブラリー / デコンボルーション / モノクローナル抗体 / ポジションスキャン / Peptide libraries / Deconvolution / Monoclonal antibodies / Position scanning / 成人基本健康診査 / CPITN / Porphyromonas gingivalis / Actinobacillus actinomycetemcomitans / 線毛 / 血液検査 / Actynobacillus actinomycetemcomitance / General Health Examination / Periodontal Diseases / Porphylomonas Gingivalis / Actinobacillus Actinomycelemcomitans / Fimbriae / Peripheral Blood Test / 歯垢 / 歯性病巣感染 / Streptococcus oralis / デンタルプラーク / 歯性病巣感染症 / ラット / infective endocarditis / dental plaque / focal infection / 天然ハーブ / キャッツクロー / 抗菌作用 / 抗炎症作用 / 抗酸化作用 / 歯周病予防 / 歯学 / 歯数 / ゲノムワイド関連解析 / 一塩基多型 / 口腔保健 / 疫学研究 / 遺伝子型 / 遺伝子環境交互作用 / 喪失歯数 / コホート研究 / 喪失歯

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有機合成化学を起点とするセンシング技術の開発と応用（難波康祐）

メタマテリアルを用いた高感度ガスセンシング技術の開発

健康長寿社会の実現に資する高感度歯科口臭検査法の開発

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伊藤博夫