研究成果

[雑誌論文] Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method

Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada KI, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y. 2013

[雑誌論文] Myosin phosphatase is inactivated by caspase-3 cleavage and phosphorylation of myosin phosphatase targeting subunit 1 during apoptosis

Iwasaki T, Katayama T, Kohama K, Endo Y, Sawasaki T 2013

[雑誌論文] Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu.

Miyakawa K., et al., 2012

[雑誌論文] A novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity.

Kiba A., et al., 2012

[雑誌論文] Stress-Inducible Caspase Substrate TRB3 Promotes Nuclear Translocation of Procaspase-3

Shimizu K, Takahama S, Endo Y, Sawasaki T 2012

[雑誌論文] Molecular and enzymatic characterization of XMRV protease by a cell-free proteolytic analysis.

Matsunaga S, Sawasaki T, Ode H, Morishita R, Furukawa A, Sakuma R, Sugiura W, Sato H, Katahira M, Takaori-Kondo A, Yamamoto N, Ryo A 2012

[雑誌論文] Interferon-Induced SCYL2 Limits Release of HIV-1 by Triggering PP2A-Mediated Dephosphorylation of the Viral Protein Vpu.

Miyakawa K, Sawasaki T, Matsunaga S, Tokarev A, Quinn G, Kimura H, Nomaguchi M, Adachi A, Yamamoto N, Guatelli J, Ryo A 2012

[雑誌論文] novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity.

Kiba A, Nakano M, Vincent-Pope P, Takahashi H, Sawasaki T, Endo Y, Ohnishi K, Yoshioka H, Hikichi Y. A 2012

[雑誌論文] The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model

Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K 2012

[雑誌論文] Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system.

Takahashi H, Ozawa A, Nemoto K, Nozawa A, Seki M, Shinozaki K, Takeda H, Endo Y, Sawasaki T. 2012

[雑誌論文] Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system.

Nemoto K, Seto T, Takahashi H, Nozawa A, Seki M, Shinozaki K, Endo Y, Sawasaki T. 2011

[学会発表] Caspase cleavage of ER stress-inducible TRB3 is a key switch from survival to cell death

Kouhei Shimizu,Shoukichi Takahama, Tatsuya Sawasaki and Yaeta Endo 2011

[雑誌論文] Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system.

Nozawa A, Ogasawara T, Matsunaga S, Iwasaki T, Sawasaki T, Endo Y 2011

[学会発表] Absolute quantitative analysis of mouse transmebrane proteome using a combination of wheat cell-free synthesis and MRM mass spectrometry

Nobuaki Takemori, Ayako Takemori, Kazuhiro Matsuoka, Natsuki Matsushita, Hiroyuki Takeda, Tatsuya Sawasaki, Yaeta Endo and Shigeki Higashiyama 2011

[雑誌論文] Specific In Situ Visualization of Plasma Cells Producing Antibodies Against Porphyromonas Gingivalis in Gingival Radicular Cyst: Application of the Enzyme-Labeled Antigen Method.

Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada KI, Tsutsumi Y. 2011

[雑誌論文] Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules.

Makino S, Sawasaki T, Endo Y, Takai K 2011

[学会発表] An attempt to prepare membrane proteins using the wheat cell-free protein production system

Yaeta Endo, Tatsuya Sawasaki, Lassale Michael W. 2011

[学会発表] コムギ無細胞タンパク質発現系を用いた植物チロシンキナーゼ(PPTK)の網羅的探索・同定および機能解析

根本圭一郎, 関原明, 篠崎一雄, 遠藤弥重太, 澤崎 達也 2011

[学会発表] AN ABSOLUTE QUANTIFICATION METHOD OF MOUSE TRANSMEMBRANE PROTEOME USING A COMBINATION OF WHEAT CELL-FREE SYNTHESIS AND MRM MASS SPECTROMETRY

N. Takemori, A. Takemori, K. Matsuoka, N. Matsushita, T. Sawasaki, Y. Endo, S. Higashiyama 2011

[学会発表] Kinome-wide screening reveals Sgk495 as a novel HCV NS3 substrate

Shoukichi Takahama, Masaru Arimoto, Saki Miyajima, Takahiro Masaki, Keiichirou Nemoto, Takanobu Kato, Takaji Wakita, Tetsuro Suzuki, Yaeta Endo and Tatsuya Sawasaki 2011

[学会発表] I CONSTRUCTION OF INFLUENZA VIRUS-LIKE PARTICLES USING A LIPOSOME-SUPPLEMENTED WHEAT CELL-FREE TRANSLATION SYSTEM

Atsushi Muroi, Tomio Ogasawara, Kyoko Shinya, Akiko Makino, Teridah E Ginting, Yaeta Endo, Tatsuya Sawasaki 2011

[学会発表] Mechanical Regulation and Implication of Cancer: Oncogene-Induced Cell Transformation Involves Proteolysis of the Mechano-Sensing Protein p130Cas

Keiko Kawauchi, Tan Song Hui, Gao Rong, Guo Alvin, Katsuhiko Iwasaki, Ichiro Harada, Hirotaka Takahashi, Tatsuya Sawasaki, Yaeta Endo, Yasuhiro Sawada 2011

[学会発表] AN IN VITRO CLEAVAGE ASSAY SYSTEM FOR XMRV PROTEASE BY WHEAT-GERM CELL FREE PROTEIN PRODUCTIO

Satoko Matsunaga, Ayako Furukawa, Yoshitsugu Kojima, Ryo Morishita, Tatsuya Sawasaki, Akifumi Takaori-Konda, Wataru Sugiura, Masato Katahira, Akihide Ryo 2011

[雑誌論文] Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling.

Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T 2011

[学会発表] IMMUNE EVASION BY HIV-1PROTEASE-MEDIATED CLEAVAGE OF TANK-BINDING KINASE 1

Tomohiro Kanuma, Ayumi Kudoh, Nao Jounai, Fumihiko Takeshita, Tatsuya Sawasaki, Akihide Ryo 2011

[雑誌論文] Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.

Matsuoka K, Komori H, Nose M, Endo Y, Sawasaki T. 2010

[雑誌論文] Practical cell-free protein synthesis system using purified wheat embryos.

Takai K, Sawasaki T, Endo Y. 2010

[学会発表] Identification of a Host Factor Antagonizing Vpu-Mediated BST-2 /Tetherin Down-Regulation

K. Miyakawa, T. Sawasaki, S. Matsunaga, N. Yamamoto, A. Ryo 2010

[学会発表] Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system

Keiichirou Nemoto, Takuya Seto, Motoaki Seki, Kazuo Shinozaki, Yaeta Endo and Tatsuya Sawasaki 2010

[学会発表] 無細胞タンパク質合成系を用いた植物のカルシウム依存リン酸化制御機構の解明

有村源一郎, Chidananda Nagamangala Kanchiswam, 高橋宏隆, 吉岡博文, 遠藤弥重太, 澤崎 達也 2010

[雑誌論文] An efficient approach to the production of vaccines against the malaria parasite.

Tsuboi T, Takeo S, Sawasaki T, Torii M, Endo Y 2010

[学会発表] An In Vitro Cleavage Assay System for XMRV Protease by Wheat-Germ Cell Free Protein Production.

S. Matsunaga, Y. Kojima, R. Morishita, T. Sawasaki, A. Ryo 2010

[学会発表] 細胞死に伴う小胞形成の時系列的変動の解析

酒巻和弘,坂本尚久,河村拓馬,千場久美子,澤崎 達也,小山田耕二 2010

[雑誌論文] Arabidopsis CPK3 plays extensive roles in various biological and environmental responses.

Arimura GI., Sawasaki T. 2010

[学会発表] Caspase-3,6,7により切断されるプロテインカイネースの比較

林祥太、清水康平、橋本季明、吉川潮、遠藤弥重太、澤崎 達也 2010

[学会発表] Development of cell-free based proteoliposome technology for delivering membrane proteins to cells

Mamoru Takahashi, Saki Miyajima, Tomio Ogasawara, Yaeta Endo, Tatsuya Sawasaki 2010

[雑誌論文] Cell-free protein synthesis for structure determination by X-ray crystallography.

Watanabe M, Miyazono K, Tanokura M, Sawasaki T, Endo Y, Kobayashi I. 2010

[学会発表] TRB3 が caspase-3 に切断されるかどうかは細胞生存と細胞死のターニングポイントの一つである

清水 康平, 高濱 正吉, 遠藤 弥重太, 澤崎 達也 2010

[雑誌論文] In vitro dissection revealed that the kinase domain of wheat RNA ligase is physically isolatable from the flanking domains as a non-overlapping domain enzyme.

Makino S, Sawasaki T, Endo Y, Takai K 2010

[学会発表] コムギ無細胞系を基盤としたがん化促進ユビキチン化 E3 リガーゼの探索

安岡佐起, 遠藤弥重太, 澤崎 達也 2010

[学会発表] タンパク質ライブラリーを用いた自己抗体プロファイリング

澤崎 達也, 松岡和弘, 遠藤 弥重太 2010

[学会発表] Caspase-3 によるストレス応答性TRB3 の切断は活性型 Akt を減衰しCaspase-3 を活性化する

清水康平、高濱正吉、遠藤弥重太、澤崎 達也 2010

[学会発表] Downregulation of myosin phosphatase targeting subunit 1 during apoptosis

K Takahiro Iwasaki, Yaeta Endo and Tatsuya Sawasaki 2010

[学会発表] Regulation of Myosin Phosphatase during Apoptosis

岩崎隆宏,遠藤弥重太,澤崎 達也 2010

[学会発表] HCV NS5A 蛋白のリン酸化に関与する新規セリン/ スレオニンプロテインキナーゼの同定と機能解析

政木 隆博,松永 智子,高橋 宏隆,加藤 孝宣,遠藤 弥重太,澤崎 達也,脇田 隆字, 鈴木 哲朗 2010

[学会発表] Screening of cancer-related E3 ubiquitin ligases by wheat cell-free system

Saki Yasuoka, Yaeta Endo, Tatsuya Sawasaki 2010

[学会発表] 酵素活性を指標とした HIV プロテアーゼ薬剤耐性新規検査法の開発

正岡 崇志, 杉浦 亙, 澤崎 達也, 松永 智子, 遠藤 弥重太, 巽 正志, Robert Shafer, 山本 直樹, 梁 明秀 2010

[雑誌論文] The Wheat-Germ Cell-Free Expression System.

Takai K, Sawasaki T, Endo Y. 2010

[学会発表] アポトーシス時におけるミオシンホスファターゼ活性調節機構

岩崎 隆宏, 遠藤 弥重太, 澤崎 達也 2010

[学会発表] コムギ無細胞系を基盤としたがん化促進ユビキチン化 E3 リガーゼの探索

安岡左起、佐々木敦朗、遠藤弥重太、澤崎 達也 2010

[学会発表] Caspase-3 cleavage of stress-responsible TRB3 reduces Akt activity and increases caspase-3 activity

Kouhei Shimizu, Shoukichi Takahama, Yaeta Endo and Tatsuya Sawasaki 2010

[学会発表] 無細胞系を用いた機能プロテオミクス技術とウイルス研究への応用

澤崎達也 2010

[学会発表] 無細胞基盤プロテオリポソームを用いた細胞への膜タンパク質導入技術の開発

高橋守, 宮島早紀, 小笠原富夫, 遠藤弥重太, 澤崎 達也 2010

[雑誌論文] Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins.

Matsunaga S, Matsuoka K, Shimizu K, Endo Y, Sawasaki T. 2010

[学会発表] Caspase-3,6,7 により切断されるプロテインカイネースの比較

林祥太, 清水康平, 橋本季明, 吉川潮, 鎌田真司, 遠藤弥重太, 澤崎 達也 2010

[学会発表] 植物 Protein Kinase ライブラリーを用いたPlant Protein Tyrosine Kinase の探索と同定

根本圭一郎, 瀬藤拓也, 関原明, 篠崎一雄, 遠藤弥重太, 澤崎 達也 2010

[雑誌論文] Wheat germ cell-free protein production system for post-genomic research.

Madono M, Sawasaki T, Morishita R, Endo Y. 2010

[雑誌論文] Evaluating the role of rheumatoid factors for the development of rheumatoid arthritis in a mouse model with a newly established ELISA system.

Tanaka Y, Komori H, Mori S, Soga Y, Tsubaki T, Terada M, Miyazaki T, Fujino T, Nakamura S, Kanno H, Sawasaki T, Endo Y, Nose M 2010

[学会発表] Screening of Cancer-Related E3 Ubiquitin Ligase by Wheat Cell-Free System

S. Yasuoka, Y. Endo, T. Sawasaki 2010

[学会発表] Development of a Cell-Free Based Screening Method to Identify Cancer Specific Autoantigen Proteins

K. Nishimori, K Matsuoka, Y. Endo, T. Sawasaki 2010

[学会発表] Biochemical comparison among of Caspase-3,6,7-cleaved protein kinases

Shouta Hayashi, Kouhei imizu, Toshiaki Hashimoto, Ushio Kikkawa, Shinji Kamada, Yaeta Endo and Tatuya Sawasaki 2010

[雑誌論文] Cell-free-based protein microarray technology using agarose/DNA microplate

Sawasaki T, Endo Y. 2010

[学会発表] 無細胞系を用いた機能プロテオミクス技術とウイルス研究への応用

澤崎 達也, 遠藤 弥重太 2010

[雑誌論文] Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system.

Sawasaki T, et al. 2010

[雑誌論文] Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system.

Tadokoro D, Takahama S, Shimizu K, Hayashi S, Endo Y, Sawasaki T. 2010

[学会発表] 無細胞基盤プロテオリポソームを用いた細胞への膜タンパク質導入技術の開発

高橋守、宮島早紀、小笠原富夫、遠藤弥重太、澤崎 達也 2010