トップ研究者を探す光合成を利用したバイオテクノロジーによる水素生産技術の研究開発

光合成を利用したバイオテクノロジーによる水素生産技術の研究開発

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-21612007
研究種目 基盤研究(C)
研究分野 バイオマスエネルギー
研究機関 愛媛大学
代表研究者 杉浦 美羽
研究期間 開始年月日 2009/4/1
研究期間 終了年度 2011
研究ステータス 完了 (2011/4/1)
配分額(合計) 4,810,000 (直接経費 :3,700,000、間接経費 :1,110,000)
配分額(履歴) 2011年度:1,560,000 (直接経費 :1,200,000、間接経費 :360,000)
2010年度:1,820,000 (直接経費 :1,400,000、間接経費 :420,000)
2009年度:1,430,000 (直接経費 :1,100,000、間接経費 :330,000)
キーワード 光合成電子伝達系
光合成電子伝達
光化学系II
酸素発生
副次的電子移動
好熱性ラン藻
部位特異的変異
反応中心

研究成果

[雑誌論文] Environment of TyrZ in Photosystem II from Thermosynechococcus elongatus in which PsbA2 Is the D1 Protein

Sugiura M, Ogami S, Kusumi M, Un S, Rappaport F, Boussac A 2012

[雑誌論文] Environment of TyrZ in Photosystem II from Thermosynechococcus elongatus in which PsbA2 is the D1 protein

Sugiura M, Ogami S, Kusumi M, Un S, Rappaport F, Boussac A. 2012

[雑誌論文] Deactivation processes in PsbA1-Photosystem II and PsbA3-Photosystem II under photoinhibitory conditions in the cyanobacterium Thermosynechococcus elongates

Ogami S, Boussac A, Sugiura M 2012

[学会発表] 酸素発生複合体におけるCa2+/Sr2+およびCl-/Br-置換が光化学系IIのエナジェティックスに及ぼす影響

山本昌一, 芝本匡雄, 加藤祐樹, 杉浦美羽, Alain Boussac, 渡辺正 2012

[雑誌論文] Deactivation processes in PsbA1-Photosystem II and PsbA3-Photosystem II under photoinhibitory conditions in the cyanobacterium

Ogami, S., Boussac, A., and Sugiura, M. 2012

[学会発表] 光合成による水の酸化速度と電子伝達コファクターとの関係

杉浦美羽 2012

[学会発表] Energy Conversion by Photosystem II and Application to Energy Creation

Miwa Sugiura, Michele Vittadello, Paul, G.Falkovski, Alain Boussac 2012

[雑誌論文] Ca^<2+> determines the entropy changes associated with the formation of transition states during water oxidation by Photosystem II

Rappaport, F., Ishida, N., Sugiura, M., Boussac, A. 2011

[学会発表] 光合成研究の最前線と課題

杉浦美羽 2011

[雑誌論文] Semiquinone-iron complex of Photosystem II : EPR signals assigned to the low-field edge of the ground state doublet of QA・-Fe2+and QB・-Fe2+

Sedoud, A., Cox, N., Sugiura, M., Lubitz, W., Boussac, A., Rutherford, W.A. 2011

[雑誌論文] 1064 nm deep near-infrared (NIR) excited Raman microspectroscopy for studying photolabile organisms.

Ando, M., Sugiura, M., Hayashi, H., Hamaguchi, H.-O. 2011

[雑誌論文] Probing the role of chloride in Photosystem II from Thermosynechococcus elongatus by exchanging chloride for iodide

Boussac A, Ishida N, Sugiura M, Rappaport F 2011

[学会発表] 光化学系II複合体における小サブユニットタンパク質の役割

杉浦美羽, 原田紗代, 岩井恵理 2011

[学会発表] Comparison of Thermosynechococcus elongatus PSII composed of different D1

Miwa Sugiura 2011

[雑誌論文] 水の酸化を伴う光合成によるエネルギー変換機構

杉浦美羽 2011

[学会発表] 光化学系II複合体の電子伝達と光の電気エネルギーへの変換

杉浦美羽, Michel Vittadello, Paul G.Falkovski 2011

[学会発表] 光合成の高効率エネルギー変換機構と新エネルギー創製への応用

杉浦美羽 2011

[雑誌論文] The effect of Ca^<2+>/Sr^<2+> substitution on the electronic structure of the oxygen-evolving complex of photosystem II : A combined multi-frequency EPR, ^<55>Mn-ENDOR and DFTstudy of the S_2 state

Cox, N., Rapatskiy, L., Su, J.-H., Pantazis, D.A., Sugiura, M., Leonid, K., Dorlet, P, Rutherford, A.W., Neese, F., Boussac, A., Lubitz, L., Messinger, J. 2011

[雑誌論文] Ca2+ determines the entropy changes associated with the formation of transition states during water oxidation by Photosystem II

Rappaport, F., Ishida, N., Sugiura, M, Boussac, A. 2011

[学会発表] Energetic comparison of Thermosynechococcus elongatus Photosystem II composed of either PsbA1 or PsbA3

Miwa Sugiura, Fabrice Rappaport, Yuki Kato, Takumi Noguchi, Alain Boussac 2011

[雑誌論文] Probing the quinone binding site of Photosystem II from Thermosynechococcus elongatus containing either PsbA1 or PsbA3 as the D1 protein through the binding characteristics of herbicides

Boussac, A., Sugiura, M. and Rappaprt, F. 2011

[学会発表] Recent progress in Photosystem II research using mutagenesis of Thermosynechococcus elongatus

Miwa Sugiura 2011

[学会発表] 光合成によるエネルギー変換と水の酸化機構

杉浦美羽 2011

[雑誌論文] 光合成による高効率エネルギー変換と水の酸化機構の解明,連載2新エネルギーへの挑戦:人工光合成をめざして

杉浦美羽 2011

[雑誌論文] Probing the quinone binding site of Photosystem II from Thermosynechococcus elongatus containing either PsbA1 or PsbA3 as the D1 protein through the binding characteristics of herbicides

Boussac, A., Sugiura, M., Rappaprt, F. 2011

[雑誌論文] 1064 nm deep near-infrared (NIR) excited Raman microspectroscopy for studying photolabile organisms

Ando, M., Sugiura, M., Hayashi, H., Hamaguchi, H.-O. 2011

[雑誌論文] Photoelectron generation by photosystem II core complexes tethered to gold surfaces

Vittadello, M., Gorbunov, M. Y., Mastrogiovanni, D. T., Wielunski, L. S., Garfunkel E. L., Guerrero F., Kirilovsky D., Sugiura, M., Rutherford, A. W., Safari A., and Falkowski, P. G. 2010

[雑誌論文] Differences in the interactions between the subunits of Photosystem II dependant on D1 protein variants in the thermophilic cyanobacterium Thermosynechococcuselongatus

Sugiura, M., Iwai, E., Hayashi, H. and Boussac, A. 2010

[学会発表] Psb30はPSII複合体において構造の安定保持を担う

原田紗代, Alain Boussac, 林秀則, 杉浦美羽 2010

[学会発表] D1:1とD1:3で構成される Thermosynechococcus elongatus の光化学系II複合体の分子構造と機能の比較

杉浦美羽, Fabrice Rappaport, 加藤祐樹, Alain Boussac 2010

[雑誌論文] Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus

Sugiura, M., Harada, S., Manabe, T., Hayashi, H., Kashino, Y., Boussac, A. 2010

[学会発表] Thermosynechococcus elongatus の光化学系II一次電子受容体フェオフィチンaの酸化還元電位:コアタンパク質D1:1とD1:3の違いが電位に及ぼす影響について

加藤祐樹, 杉浦美羽, 渡辺正 2010

[学会発表] Structural stability of Photosystem II complex composed of either PsbA1 or PsbA3 PsbA1もしくはPsbA3で構成される光化学系II複合体の構造的な安定性の違い

Miwa Sugiura Yhki Kato, Alain Boussac3 2010

[学会発表] 分光電気化学法による光化学系II電子受容分子の酸化還元電位計測

加藤祐樹, 芝本匡雄, 杉浦美羽, 渡辺正 2010

[学会発表] 光化学系IIにおけるチロシンY_ZとD1-H190の相互作用

高橋亮太, 杉浦美羽, Alain Boussac, 野口巧 2010

[学会発表] 分光電気化学的手法による光化学系II第一キノン電子受容体Q_Aの酸化還元電位計測

芝本匡雄, 加藤祐樹, 杉浦美羽, 渡辺正 2010

[雑誌論文] Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus

Sugiura, M., Harada, S., Manabe, T., Hayashi, H., Kashino, Y. and Boussac, A. 2010

[学会発表] Spectroelectrochemical determination of the redox potentials of electron acceptors, pheophytin α and primary quinone QA, in photosystem II

Yuki Kato, Tadao Shibamoto, Akinori Oda, Miwa Sugiura, Tadashi Watanabe 2010

[学会発表] 光化学系II複合体タンパク質の分子構造と機能の関係

杉浦美羽 2010

[雑誌論文] Photoelectron generation by photosystem II core complexes tethered to gold surfaces

Vittadello, M., Gorbunov, M.Y., Mastrogiovanni, D.T., Wielunski, L.S., Garfunkel E.L., Guerrero F., KirilovskyD., Sugiura, M., Rutherford, A.W., Safari A., Falkowski, P.G 2010

[雑誌論文] Photoelectron generation by photosystem II core complexes tethered to gold surfaces

Vittadello, M., Gorbunov, M.Y., Mastrogiovanni, D.T., Wielunski, L.S., Garfunkel E.L., Guerrero F., Kirilovsky D., Sugiura, M., Rutherford, A.W., Safari A., Falkowski, P.G. 2010

[雑誌論文] Hydrogen bond interactions of the pheophytin electron acceptor and its radical anion in photosystem II as revealed by Fourier transform infrared difference spectroscopy

Shibuya, Y, Takahashi, R., Okubo, T., Suzuki, H., Sugiura, M., Noguchi, T. 2010

[雑誌論文] Hydrogen bond interactions of the pheophytin electron acceptor and its radical anion in photosystem II as revealed by Fourier transform infrared difference spectroscopy.

Shibuya, Y, Takahashi, R., Okubo, T., Suzuki, H., Sugiura, M., Noguchi, T. 2010

[雑誌論文] Differences in the interactions between the subunits of Photosystem II dependant on D1 protein variants in the thermophilic cyanobacterium Thermosynechococcus elongatus

Sugiura, M., Iwai, E., Hayashi, H., Boussac, A. 2010

[雑誌論文] Psb30 contributes to structurally stabilise the Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus

Sugiura, M., Harada, S., Manabe, T., Hayashi, H., Kashino, Y., Boussac, A. 2010

[学会発表] 分光電気化学計測による光化学系II電子伝達分子のエネルギー準位相関の解明

加藤祐樹, 芝本匡雄, 杉浦美羽, 渡辺正 2010

[学会発表] Molecular structure and function of Thermosynechococcus elongatus Photosystem II composed of either D1:1 or D1:3

Miwa Sugiura, Fabrice Rappaport, Yuki Kato, Takumi Noguchi, Alain Boussac 2010

[学会発表] Energetics within photosystem II based on the redox potentials of pheophytin α and primary quinone QA determined by spectroelectrochemistry

Yuki Kato, Miwa Sugiura, Tadashi Watanabe 2010

[学会発表] 1064nm励起顕微ラマン分光法によるシアノバクテリアの in vivo 測定

安藤正浩, 杉浦美羽, 林秀則, 浜口宏夫 2010

[学会発表] 分光電気化学的手法により計測した光化学系IIフェオフィチンaの酸化還元電位

加藤祐樹, 杉浦美羽, 渡辺正 2009

[雑誌論文] Hydrogen bond interactions of the pheophytin electron acceptor and its radical anion in photosystem II as revealed by Fourier transform infrared difference spectroscopy

Shibamoto, T., Kato, Y., Sugiura, M., Watanabe, T. 2009

[雑誌論文] Structural coupling of a tyrosine side chain with the non-heme iron center in Photosystem II as revealed by light-induced Fourier transform infrared difference spectroscopy

Takahashi, R., Boussac, A., Sugiura, M., Noguchi, T. 2009

[学会発表] 好熱性ラン藻のD1:3は恒常的に発現しているD1:1よりも安定な光化学系II複合体を構成する

杉浦美羽, 原田紗代, 岩井恵理, 真鍋敬, 林秀則, Alain Boussac 2009

[学会発表] Influence of D1-H332, a ligand to the Mn_4Ca-cluster, in the water oxidation mechanism of the oxygen-evolving Photosystem II enzyme

Miwa Sugiura, Yohei Ohno, Fabrice Rappaport, Hiroyuki Suzuki, Takumi Noguchi, Hidenori Hayashi, Alain Boussac 2009

[雑誌論文] Probing the coupling between proton and electron transfer in Photosystem II core complexes containing a 3-fluorotyrosine

Rappaport, F.Boussac, A., Force, D.A., Peloquin, J., Brynda, M., Sugiura, M., Un, U., Britt, R., Diner, B. 2009

[学会発表] Affinity of structurally modified metalothionein to heavy metals

Shin-ichi Takeuchi, Miwa Sugiura, Hidenori Hayashi 2009

[学会発表] QA再構成とFTIR解析によるPSIIにおける第一電子受容体QAの分子間相互作用の解析

梢雄多, 高野晃, 杉本育代, 鈴木博行, 杉浦美羽, 高橋裕一郎, 野口巧 2009

[雑誌論文] Complete EPR spectrum of the S3-state of the oxygen-evolving Photosystem II

Boussac, A., Sugiura, M., Rutherford, A.W., Dorlet, P. 2009

[雑誌論文] Evidence that D1-His332 in Photosystem II from Thermosynechococcus elongatus interacts with the S_3-state and not with the S_2-state

Sugiura, M., Rappaport, F., Hillier, W., Dorlet, P., Ohno, Y., Hayashi, H., Boussac, A. 2009

[雑誌論文] Monitoring proton release during photosynthetic water oxidation in photosystem II by means of isotope-edited infrared spectroscopy

Suzuki, H., Sugiura, M., Noguchi, T. 2009

[雑誌論文] Spectroelectrochemical determination of the redox potential of Pheophytin a, the primary electron acceptor in Photosystem II

Kato, Y., Sugiura, M., Oda, A., Watanabe, T. 2009

[学会発表] Hydrogen bond network around the non-heme iron in Photosystem II

高橋亮太, 杉浦美羽, Alain Boussac, 野口巧 2009

[雑誌論文] Spectroelectrochemical determination of the redox potential of Pheophytin a, the primary electron acceptor in Photosystem II

Kato, Y., Sugiura, M., Oda, A. and Watanabe, T. 2009

[雑誌論文] Evidence that D1-His332 in Photosystem II from Thermosynechococcus elongatus interacts with the S_3-state and not with the S_2-state

Sugiura, M., Rappaport, F., Hillier, W., Dorlet, P., Ohno, Y., Hayashi, H. and Boussac, A. 2009

[学会発表] 異なるアミノ酸を持つ反応中心タンパク質で構成される光化学系II複合体のエネルギー論的な比較

杉浦美羽, Fabrice Rappaport, 加藤祐樹, Alain Boussac 2009

[雑誌論文] 酸素発生型光合成微生物による光-水素生成

杉浦美羽 2009

[学会発表] 異なる反応中心D1タンパク質で構成された光化学系II複合体タンパク質の分子構造と光合成機能

杉浦美羽 2009

[学会発表] FTIR detection of the DOD bending vibrations of water molecules involved in the photosynthetic water oxidation

鈴木博行, 杉浦美羽, 野口巧 2009

[雑誌論文] Monitoring proton release during photosynthetic water oxidation in photosystem II by means of isotope-edited infrared spectroscopy

Suzuki, H., Sugiura, M. and Noguchi, T. 2009

[雑誌論文] Complete EPR spectrum of the S_3-state of the oxygen-evolving Photosystem II

Boussac, A., Sugiura, M., Rutherford, A. W. and Dorlet, P. 2009

[学会発表] Metallothioneins expressed in the lumen and stroma of thylakoids of a thermophilic cyanobacterium Thermosynechococcus elongatus

Hiroshi Nakano, Takashi Manabe, Hidenori Hayashi, Miwa Sugiura 2009

[学会発表] Recent Progress in the Photosytem II enzyme

杉浦美羽 2009