定量メタバーコーディングによる迅速で正確な種多様性評価：群集と環境DNAへの適用

トップ›研究者を探す›定量メタバーコーディングによる迅速で正確な種多様性評価：群集と環境DNAへの適用

定量メタバーコーディングによる迅速で正確な種多様性評価：群集と環境DNAへの適用

代表研究者	渡辺幸三
研究課題番号	KAKENHI-PROJECT-19H02276
研究種目	基盤研究(B)
研究分野
研究機関	愛媛大学
研究分担者	加藤幹男
研究分担者	竹門康弘
研究期間開始年月日	2019/4/1
研究期間終了年度	2021
研究ステータス	完了 (2021/4/1)
配分額（合計）	17,290,000 (直接経費 :13,300,000、間接経費 :3,990,000)
配分額（履歴）	2021年度：5,330,000 (直接経費 :4,100,000、間接経費 :1,230,000) 2020年度：5,590,000 (直接経費 :4,300,000、間接経費 :1,290,000) 2019年度：6,370,000 (直接経費 :4,900,000、間接経費 :1,470,000)
キーワード	河川生物多様性メタバーコーディング底生動物環境DNA

研究成果

[雑誌論文] Sediment-associated Microbial Community Profiling: Sample Pre-processing through Sequential Membrane Filtration for 16s rDNA Amplicon Sequencing

Joeselle M. Serrana, Kozo Watanabe 2022

[学会発表] 高見川(奈良県)で採集した水生昆虫の次世代シーケンサーによる種判別

稲井公二・脇村圭・渡辺幸三・加藤幹男 2022

[雑誌論文] Notes on the Taxonomic Status and Distribution of Some Cylindrotomidae (Tipuloidea, Diptera), with Emphasis on Japanese Species

Levente-Peter Kolcsar, Nikolai Paramonov, Yume Imada, Daichi Kato, Maribet Gamboa, Dai Shinoka, Makoto Kato, Kozo Watanabe 2022

[雑誌論文] Spatially Varying Trophic Effects of Reservoir-Derived Plankton on Stream Macroinvertebrates Among Heterogeneous Habitats Within Reaches

Shinji Takahashi, Yasuhiro Takemon, Tatsuo Omura, Kozo Watanabe 2022

[雑誌論文] Implications of taxonomic and numerical resolution on DNA metabarcoding-based inference of benthic macroinvertebrate responses to river restoration

Joeselle M. Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2022

[雑誌論文] Detailed Description and Illustration of Larva, Pupa, and Imago of Holorusia mikado (Westwood, 1876) (Diptera: Tipulidae) from Japan

Kolcsar, L-P, D. T. Nakamura, Kato, and K. Watanabe 2021

[学会発表] Contrasting Adaptive Genetic Consequences of Stream Insects under Changing Climate

Nukazawa K., Chiu M-C., Kazama S., Watanabe K. 2021

[学会発表] Elevational Patterns of Genetic Diversity in Riverine Populations in Japan

Chiu M. C, Watanabe K 2021

[学会発表] Haplotype-level DNA metabarcoding from freshwater macroinvertebrate community samples

Joeselle M. Serrana and Kozo Watanabe 2021

[学会発表] Taxonomic Revision of Japanese Snow Crane Flies (Chionea, limoniidae): A First Step to Understand the Effect of Climate Change on Winter Active Insect

Kolcsar L.P., Kato D. Watanabe K. 2021

[学会発表] Taxonomic Revision of Japanese Snow Crane Flies (Chionea, limoniidae): A First Step to Understand the Effect of Climate Change on Winter Active Insects

Kolcsar L.-P, Kato D., Watanabe K. 2021

[学会発表] 高見川(奈良県)で採取した水生昆虫の次世代シーケンサーによる種判別

稲井公二，脇村圭，渡辺幸三，加藤幹男 2021

[雑誌論文] Contribution to the Knowledge of Limoniidae (Diptera: Tipuloidea): First Records of 244 Species from Various European Countries

Kolcsar L-P, P. Oosterbroek, D. I. Gavryushin, K. M. Olsen, N. M. Paramonov, V. Pilipenko, J. Stary, A. Polevoi, E. Eiroa, M. Andersson, J. Salmela, C. Quindroit, M. C. dOliveira, E. G. Hancock, J. Mederos, P. Boardman, E. Viitanen and K. Watanabe 2021

[学会発表] 吉野川(奈良県)で採集した水生昆虫のメタバーコーディング解析

稲井公二・脇村圭・渡辺幸三・加藤幹男 2021

[雑誌論文] Loss of a larval generic character: an interesting and new description for Isoperla vevcianensis Ikonomov, 1980 (Plecoptera: Perlodidae) with updated adult characters

D?VID MUR?NYI, TIBOR KOV?CS, MARIBET GAMBOA, KOZO WATANABE 2021

[学会発表] Phylogeny and taxonomic revision of Palearctic Cylindrotomidae (Diptera)

Levente-Peter Kolcsar, Yume Imada, Kozo Watanabe 2021

[雑誌論文] Profiling the Microbial Community Structure and Functional Diversity of a Dam-regulated River Undergoing Gravel Bar Restoration

Joeselle M. Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2021

[雑誌論文] Detection of Adaptive Divergence in Populations of the Stream Mayfly Ephemera strigata with Machine Learning

Li, B., S. Yaegashi, T. M. Carvajal, M. Gamboa and K. Watanabe 2020

[雑誌論文] Synopsis of the genus Ulomorpha Osten Sacken, 1869 (Diptera, Limoniidae) in Japan

Kato, D., K. Watanabe, and L-P Kolcs?r 2020

[雑誌論文] Spatial Genetic Structure of the Invasive Riparian Tree Robinia pseudoacacia to Determine Migration Patterns to Inform Best Practices for Riparian Restoration

Yaegashi, S., T. Omura, and K. Watanabe 2020

[学会発表] Microbial community diversity across hyporheic zoes of gravel bars: taxonomic and fucntio nal metrics

Arnelyn Doloiras-Larano,Maribet Gamboa, Shinji Takahashi, Joeselle Serrana Yasuhiro Tekemon, Paul R. Johnston, Michael T. Monaghan, and Kozo Watanabe 2020

[雑誌論文] Remarkable Anoxia Tolerance by Stoneflies from a Floodplain Aquifer

Malison, R. L., B. K. Ellis, A. G. DelVecchia, H. N. Jacobson, B. K. Hand, G. Luikart, H. A. Woods, M. Gamboa, K. Watanabe, and J. A. Stanford 2020

[雑誌論文] Quorum Sensing-linked agrA Expression by Ethno-synthesized Gold Nanoparticles in Tilapia Streptococcus agalactiae Biofilm Formation

Fernando, S. I. D., K. G. J. Cruz and K. Watanabe 2020

[学会発表] Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16s rDNA amplicon sequencing

Joeselle M. Serrana and Kozo Watanabe 2020

[学会発表] Recurrent seasonal dynamics of the planktonic eukaryotic communities in the polymictic eutrophic Lake M?ggelsee (Germany).

Joeselle M. Serrana, Kingsly Chuo Beng, Jusytna Wolinska, Kozo Watanabe and Michael T. Monaghan 2020

[学会発表] Molecular Genetic Approaches to the Assessment of Dam Impact on Stream Macroinvertebrates and Dengue Virus Surveillance of Vector Mosquitoes

Kozo Watanabe 2020

[雑誌論文] Revision of Japanese species of Nipponomyia Alexander, 1924 (Diptera, Pediciidae)

Kolcsar, L. P., D. Kato, M. Gamboa, and K. Watanabe 2020

[学会発表] Molecular Approaches to the Study of Freshwater Biodiversity: Genomic Adaptation and DNA Metabarcoding.

Kozo Watanabe 2019

[学会発表] Comparison of DNA metabarcoding and morphological identification for stream macroinvertebrate biodiversity assessment and monitoring

Joeselle Serrana, Yo Miyake, Maribet Gamboa, Kozo Watanabe 2019

[学会発表] Landscape shape predicts enclosed genetic variation within the metapopulation of a species with varied dispersal

Ming-Chih Chiu, Kozo Watanabe 2019

[学会発表] 環境DNAバーコーディング解析を用いた河川水生昆虫の種多様性の解明：高知県四万十川を例として

八重樫咲子，細川大樹，渡辺幸三 2019

[学会発表] Spatial and Phylogenetic Structure of DNA-species of Alpine Stoneflies Community Assemblages across Seven Habitats

Maribet Gamboa, Joeselle Serrana, Michael Monaghan, Yasuhiro Takemon, Kozo Watanabe 2019

[学会発表] フィリピンのココヤシ寄生虫と寄生バチの2種間に生じる遺伝学的相互作用の解明

石谷直渡，Serrana, J.M.，Carvajal, T.M.，Almarinez, B.J.M.，Barrion, A.T.，Amalin, D.M.，渡辺幸三 2019

[学会発表] Protein quantitative traits loci (pQTL) expression of the stonefly Nemoura ovocercia (Plecoptera) along nationwide region of Japan

Maribet Gamboa, Maria Claret Tsuchiya, Shunsuke Yamano, Thaddeus Carvajal, Hisato Iwata, Kozo Watanabe 2019

[学会発表] 7つのハビタットにおけるカワゲラのDNA種の空間構造および系統発生構造の推定

Maribet Gamboa, Michael Monaghan, 竹門康弘, 渡辺幸三 2019

[学会発表] Genome-wide Transcriptome Analysis of stream stonefly along a climatic gradient in Japana

Arnelyn Doloiras-Larano, Gotoh Yusuke, Maribet Gamboa and Kozo Watanabe 2019

[学会発表] 四万十川と仁淀川の環境DNA及び底生動物群集のメタバーコーディング解析

石谷直渡，八重樫咲子，Serrana, J.M.，Fernando, S.I.，渡辺幸三 2019

[学会発表] Species dispersal mediates opposing influences of a branching network on genetic variation in a metapopulation

Ming-Chih Chiu, Bin Li, Kei Nukazawa, Thaddeus Carvajal, Kozo Watanabe 2019

[学会発表] Sediment Microbial Diversity and Community Composition of Restored Gravel Bars in the Trinity River, California

Joeselle Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2019