トップ研究者を探す分子シャペロンの協調メカニズムの解明

分子シャペロンの協調メカニズムの解明

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-19770137
研究種目 若手研究(B)
研究分野 生物系
生物学
生物科学
生物物理学
研究機関 独立行政法人理化学研究所
代表研究者 座古 保
研究期間 開始年月日 2007/4/1
研究期間 終了年度 2008
研究ステータス 完了 (2008/4/1)
配分額(合計) 3,790,000 (直接経費 :3,400,000、間接経費 :390,000)
配分額(履歴) 2008年度:1,690,000 (直接経費 :1,300,000、間接経費 :390,000)
2007年度:2,100,000 (直接経費 :2,100,000)
キーワード 好熱菌タンパク質
分子シャペロン
シャペロニン
プレフォルディン
分子間相互作用
リフォールディング
蛍光偏光異方性解消法
変性タンパク質
アミロイド
アップコンバージョンナノ粒子

研究成果

[図書] Heat shock proteins : New Research Chapter XXI

Zako, T. 2008

[学会発表] Development of PEGylated upconversion nanophosphors as a fluorescence bioimaging probe and its application for tumor cell imaging

Zako, T. 2008

[学会発表] Detection and diagnostics of amyloidic proteins using luminescent conjugated polymers

Mikael Lindgren, Tamotsu Zako, Mizuo Maeda, Per Hammarstrom and K. Peter R. Nilsson 2008

[雑誌論文] Dynamics of group II chaperonin and prefoldin probed by 13C NMR spectroscopy

Kurimoto, E., Nishi, Y., Yamaguchi, Y., Zako, T., Iizuka, R., Ide, N., Yohda, M. and Kato, K. 2008

[学会発表] Small heat shock protein (sHsp)はストレス条件下においてアミロイドβと複合化し無毒のアミロイド様繊維を生成する

座古保、迫野昌文、内海現太、養王田正文、前田瑞夫 2008

[図書] "Chaperonin and prefoldin - Two molecular chaperones that work cooperatively in archaea and eukaryote" (Invited Review) in Heat shock proteins : New Research (Edited by Emma Moreland and Camille Vincent)

Zako, T., Iizuka, R., Kanzaki, T., Maeda, M. and Yohda, M. 2008

[雑誌論文] Effect of the C-terminal truncation on the functional cycle of chaperonin groel : Implication that the C-terminal region facilitates the transition from the folding-arrested to the folding-competent state.

Suzuki M, Ueno T, Iizuka R, Miura T, Zako T, Akahori R, Miyake T, Shimamoto N, Aoki M, Tanii T, Ohdomari I, Funatsu T. 2008

[学会発表] Prefoldin prudentially interacts with group II chaperonin in its ADP bound open conformation

Tamotsu Zako, Yosuke Murase, Ryo Iizuka, Taro Kanzaki, Masafumi Shimizu, Masafumi Yohda and Mizuo Maeda 2008

[雑誌論文] Improvement of dispersion stability and characterization of upconversion nanophosphors covalently modified with PEG as a fluorescence bioimaging probe

Zako, T., Nagata, H., Terada, N., Sakono, M, Soga, K and Maeda, M. 2008

[雑誌論文] Improvement of dispersion stability and characterization of upcbnversion nanophosphors covalently modified with PEG as a fluorescence bioimaging probe

Zako, T. 2008

[学会発表] Small heat shock protein (sHsp)はストレス条件下においてアミロイド6と複合化し無毒のアミロイド様繊維を生成する

座古保 2008

[学会発表] Detection and diagnostics of amyloidic proteins using luminescent conjugated polymers

Zako, T. 2008

[学会発表] Formation of Novel Non-toxic Insulin Amyloid Filaments by reducing agent

Tamotsu Zako, Masafumi Sakono, Naomi Hashimoto, Masaki Ihara and Mizuo Maeda 2008

[学会発表] Development of PEGylated upconversion nanophosphors as a fluorescence bioimaging probe and its application for tumor cell imaging

Tamotsu Zako, Hiroyasu Nagata, Naofumi Terada, Arata Utsumi, Masafumi Sakono, Hiroshi Ueda, Masafumi Yohda, Kohei Soga and Mizuo Maeda 2008

[学会発表] 分子シャペロンによる新規なアミロイド形成

座古保、迫野昌文、内海現太、養王田正文、前田瑞夫 2008

[雑誌論文] Kinetic Analysis of Conformational Changes of GroEL Based on the Fluorescence of Tyrosine 506.

Hosono K, Ueno T, Taguchi H, Motojima F, Zako T, Yoshida M, Funatsu T. 2008

[学会発表] Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin

Masafumi Sakono, Tamotsu Zako, Hiroshi Ueda, Masafumi Yohda and Mizuo Maeda 2008

[雑誌論文] Complex formation of CdSe/ZnS/TOPO nanocrystal vs. molecular chaperone in aqueous solution by hydrophobic interaction

Horiuchi, H., Iwami, N., Tachibana, F., Ohtaki, A., Iizuka, R., Zako, T., Oda, M., Yohda, M. and Tani, T. 2007

[学会発表] プレフォルディンーシャペロニンの相互作用解析による基質受け渡し機構の解明

座古保 2007

[学会発表] Interaction of prefoldin with group II chaperonin in the presence of various nucleotides

Tamotsu Zako 2007

[学会発表] プレフォルディン-シャペロニンの相互作用解析による基質受け渡し機構の解明

座古保, 村瀬陽介, 清水雅史, 飯塚怜, 前田瑞夫, 養王田正文 2007

[学会発表] Interaction of prefoldin with group II chaperonin in the presence of various nucleotides

Tamotsu Zako, Yosuke Murase, Andrei Zhukov, Robert Karlsson, Masafumi Shimizu, Ryo Iizuka, Mizuo Maeda, and Masafumi Yohda 2007

[雑誌論文] Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Miyake, T, Tanii, T., Kato, K., Zako, T., Funatsu, T. and Ohdomari I. 2007

[雑誌論文] Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin

Sakono, M., Zako, T., Ueda, H., Yohda, M. and Maeda, M.