トップ研究者を探す生物による水素エネルギー生産を目指した光合成の水分解機構と分子構造に関する研究

生物による水素エネルギー生産を目指した光合成の水分解機構と分子構造に関する研究

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-18770116
研究種目 若手研究(B)
研究分野 生物系
生物学
生物科学
機能生物化学
研究機関 愛媛大学
大阪府立大学
代表研究者 杉浦 美羽
研究期間 開始年月日 2006/4/1
研究期間 終了年度 2008
研究ステータス 完了 (2008/4/1)
配分額(合計) 3,950,000 (直接経費 :3,800,000、間接経費 :150,000)
配分額(履歴) 2008年度:650,000 (直接経費 :500,000、間接経費 :150,000)
2007年度:500,000 (直接経費 :500,000)
2006年度:2,800,000 (直接経費 :2,800,000)
キーワード 生体エネルギー変換
光合成電子伝達系
酸素発生
部位特異的変異
好熱性ラン藻
光化学系II
好熱性シアノバクテリア
水素エネルギー

研究成果

[学会発表] 光化学系IIタンパク質複合体の分子構造の解析の現状

杉浦美羽 2009

[雑誌論文] Probing the coupling between proton and electron transfer in Photosystem II core complexes containing a 3-fluorotyrosine

IRappaport, F., Boussac, A., Force, D. A., Peloquin, J., Brynda, M., Sugiura, M., Un, U., Britt, R., and Diner. B 2009

[雑誌論文] 酸素発生型光合成微生物による光-水素生成

杉浦美羽 2009

[雑誌論文] Complete EPR spectrum of the S3-state of the oxygen-evolving Photosystem II

Boussac, A., Sugiura, M., Rutherford, A.W. and Dorlet, P. 2009

[雑誌論文] Probing the coupling between proton and electron transfer in Photosystem II core complexes containing a 3-fluorotyrosine

Rappaport, F. Boussac, A., Force, D. A., Peloquin, J., Brynda, M., Sugiura, M., Un, U., Britt, R. and Diner, B. 2009

[雑誌論文] Complete EPR spectrum of the S3-state of the oxygen-evolving Photosystem II

Boussac, A., Sugiura, M., Rutherford, A. W., and Dorlet, P 2009

[学会発表] 水の酸化反応におけるD1-His332の役割

杉浦美羽、大野陽平、Alain Boussac、Fabrice Rappaport、鈴木博行、野口巧、林秀則 2009

[雑誌論文] Monitoring proton release during photosynthetic water oxidation in photosystem II by means of isotope-edited infrared spectroscopy

Suzuki, H., Sugiura, M. and Noguchi, T 2009

[雑誌論文] 酸素発生型光合成微生物による光-水素生成、新エネルギーの開発の現状と将来特集

杉浦美羽 2009

[学会発表] 酸化反応におけるDl-His332の役割

杉浦美羽、大野陽平、Alain Boussac、Fabrice Rappaport、鈴木博行、野口巧、林秀則 2009

[雑誌論文] Low temperature illuminations of Photosystem II from Thermosynechococcus elongatus by visible and near-infrared lights

Boussac, A., Sugiura, M., Lai, T.-L. and Rutherford, A.W. 2008

[雑誌論文] Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803

Inoue-Kashino, N., Takahashi, T., Ban, A., Sugiura, M., Takahashi, Y., Satoh, K., and Kashino, Y 2008

[学会発表] 光化学系IIの構造と機能の解明から応用まで

杉浦美羽 2008

[雑誌論文] Monitoring water reactions during the S-state cycle of the photosynthetic water-oxidizing center: Detection of the DOD bending vibrations by means of Fourier transform infrared spectroscopy

Suzuki, H., Sugiura, M. and Noguchi, T 2008

[雑誌論文] Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P680, of Photosystem II

Sugiura, M., Boussac, A., Noguchi, T. and Rappaport, F. 2008

[雑誌論文] Analysis of xenon binding to photosystem Ii by X-ray crystallography

Murray, J.W., Maghlaoui, K., Kargul, J., Sugiura, M., and Barber, J. 2008

[雑誌論文] X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II

Murray, J.W, Maghlaoui, K., Kargul, J., Ishida, N., Lai, T.-L., Rutherford, A.W., Sugiura, M., Boussac. A. and Barber, J 2008

[学会発表] 光化学系IIにおける水の分解メカニズム

杉浦美羽 2008

[雑誌論文] Evidence for a stable association of Psb30 (Ycf12) with photosystem II core complex in the cyanobacterium Synechocystis sp. PCC 6803

Inoue-Kashino, N., Takahashi, T., Ban, A., Sugiura, M., Takahashi, Y., Satoh, K. and Kashino, Y 2008

[雑誌論文] Quantum efficiency distributions of photo-induced side-pathway donor oxidation at cryogenic temperature in Photosystem II

Hughes, J. L., Rutherford, A. W., Sugiura, M. and Krausz, E 2008

[学会発表] Effects of P_<680> ligand substitution in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus

Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport 2008

[雑誌論文] Quantum efficiency distributions of photo-induced side-pathway donor oxidation at cryogenic temperature in Photosystem II

Hughes, J. L., Rutherford, A. W., Sugiura, M. and Krausz, E. 2008

[雑誌論文] Low temperature illuminations of Photosystem II from Thermosynechococcus elongatus by visible and near-infrared lights

Boussac, A., Sugiura, M., Lai, T.-L.and Rutherford, A.W. 2008

[雑誌論文] Isotopic labelling of Photosystem II in Thermosynechococcus elongatus

Boussac, A., Verbavatzand, J.-M., and Sugiura, M. 2008

[雑誌論文] Biosynthetic Exchange of Bromide for Chloride and Strontium for Calcium in the Photosystem II Oxygen-evolving Enzymes

Ishida, N, Sugiura, M., Rappaport, F., Lai, T.-L., Rutherford, A.W., and Boussac, A 2008

[学会発表] Effects of P680 ligand substitution in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus

Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport 2008

[雑誌論文] The role of TyrD in the electron transfer kinetics in Photosystem II

Szczepaniak, M., Sugiura, M., and Holzwarth, A. R 2008

[雑誌論文] Biosynthetic Exchange of Bromide for Chloride and Strontium for Calcium in the Photosystem II Oxygen-evolving Enzymes

Ishida, N, Sugiura, M., Rappaport, F., Lai, T.-L., Rutherford, A.W., and Boussac, A. 2008

[学会発表] 酸素発生型光合成微生物による光-水素生成

杉浦美羽 2008

[雑誌論文] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mn cluster in Photosystem II

Aoyama, C., Suzuki, H., Sugiura, M. and Noguchi, T 2008

[学会発表] The water splitting enzyme: recent advances

A., Boussac, N., Ishida, T.-L., Lai, A.W,. Rutherford, F., Rappaport, Y., Pushkar, M., Sugiura 2008

[雑誌論文] Isotopic labelling of Photosystem II in Thermosynechococcus elongatus

Boussac, A., Verbavatzand, J.-M., and Sugiura, M 2008

[雑誌論文] X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II

Murray, J.W, Maghlaoui, K., Kargul, J., Ishida, N., Lai, T.-L., Rutherford, A.W., Sugiura, M., Boussac. A. and Barber, J. 2008

[雑誌論文] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mn cluster in Photosystem II

Aoyama, C., Suzuki, H., Sugiura, M. and Noguchi, T. 2008

[雑誌論文] Flash-induced FTIR Difference Spectroscopy Shows No Evidence for Structural Coupling of Bicarbonate to the Oxygen-Evolving Mncluster in Photosystem II

Aoyama, C., Suzuki, H., Sugiura, M.and Noguchi, T. 2008

[雑誌論文] Monitoring water reactions during the S-state cycle of the photosynthetic water-oxidizing center : Detection of the DOD bending vibrations by means of Fourier transform infrared spectroscopy

Suzuki, H., Sugiura, M. and Noguchi, T. 2008

[雑誌論文] Influence of Histidine-198 of the D1 subunit on the properties of the primary electron donor, P_<680>, of Photosystem II Thermosynechococcus elongatus

Sugiura, M., Boussac, A., Noguchi, T. and Rappaport, F 2008

[雑誌論文] The role of TyrD in the electron transfer kinetics in Photosystem II

Szczepaniak, M., Sugiura, M. and Holzwarth, A.R. 2008

[雑誌論文] Influence of Histidine-198 of the Dl subunit on the properties of the primary lectron donor,P_680,of Photosystem II Thermosynechococcus elongatus

Sugiura, M., Boussac, A., Noguchi, T.and Rappaport, F. 2008

[学会発表] 光化学系IIにおける水の分解メカニズム

杉浦美羽 2008

[学会発表] 酸素発生型光合成微生物による光-水素生成

杉浦美羽 2008

[学会発表] 光化学系IIの構造と機能の解明から応用まで

杉浦美羽 2008

[雑誌論文] Ycf12 is a core subunit in photosystem II complex

Kashino, Y., Ikeda, Y., Ban, A., Inoue-Kashino, N., Satoh, K. and Sugiura, M. 2007

[雑誌論文] Characterization of the tyrosine-Z radical and its environment in the spin-coupled S2TyrZ state of Photosystem II from Thermosynechococcus elongatus

Un, S., Boussac, A. and Sugiura, M. 2007

[雑誌論文] Perturbation of the structure of P680 and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by Fourier transform infrared spectroscopy

Okubo, T., Sugiura, M., Tomo, T., and Noguchi, T. 2007

[学会発表] Spectroscopic studies of the Mn_4Ca cluster in mutants and biosynthetically modified photosystem II from Thermosynechococcus elongatus

Alain Boussac and Miwa Sugiura 2007

[学会発表] FTIR study on the proton release pattern duing water oxidation in Photosystem II core complexes from Thermosynechococcus elongatus

Hiroyuki Suzuki, Miwa Sugiura, and Takumi Noguchi 2007

[雑誌論文] Water molecules coupled to the redox-active tyrosine YD in Photosystem II as detection by FTIR Spectroscopy

Takahashi, R., Sugiura, M. and Noguchi, T. 2007

[学会発表] Effects of P_680 ligand in the oxygen-evolving Photosystem II of Thermosynechococcus elongatus

Miwa Sugiura 2007

[雑誌論文] Characterization of the Tyrosine-Z radical and Its Environment in the Spin-Coupled S_2Tyr_z State of Photosystem II from Thermosynechococcus elongatus

Sun Un, Alain Boussac, Miwa Sugiura 2007

[学会発表] P_680ligand in the oxyen-evolving Photosystem II of Thermosynechococcus elongatus

Miwa Sugiura, Alain Boussac, Takumi Noguchi, and Fabrice Rappaport 2007

[学会発表] Thermosynechococcus elongatus光化学系IIにおけるP680リガンドの酸化還元電位への影響

杉浦美羽、Alain Boussac、野口巧、Fabrice Rappaport 2007

[雑誌論文] Perturbation of the structure of P_<680> and the charge distribution on its radical cation in isolated reaction center complexes of photosystem II as revealed by Fourier transform infrared spectroscopy

Okubo, T., Sugiura, M., Tomo, T., Noguchi, T. 2007

[雑誌論文] A nitrite transporter associated with nitrite uptake by higher plant chloroplasts

Sugiura, M, Georgescu, M.N.and Takahashi, M. 2007

[学会発表] Spectroscopic studies of the Mn_4Ca cluster in mutans and biosyntheticallt modified Photosystem II from Thermosynechococcus elongatus

Alain Boussaz, Miwa Sugiura, Naoko Ishida, Sun Un, Fabrice Rappaport, and A.William Rutherford 2007

[雑誌論文] Ycfl2 is a core subunit in photosystem II complex

Kashino, Y., Ikeda, Y., Ban, A., Inoue-Kashino, N., Satoh, K.and Sugiura, M. 2007

[学会発表] Influence of the redox active Tyrosine D on the electron transfer in cyanobacterial Photosytem II

Malwina Szczepaniak, Miwa Sugiura, and Alfred R.holzwarth 2007

[学会発表] Structural coupling of water molecules with Y_D in Photosystem II as revealed by FTIR spectroscopy

Ryota Takahashi, Miwa Sugiura, and Takumi Noguchi 2007

[雑誌論文] Water molecules coupled to the redox-active tyrosine Y_D in Photosystem II as detection by FTIR Spectroscopy

Takahashi, R., Sugiura, M.and Noguchi, T. 2007

[雑誌論文] A nitrite transporter associated with nitrite uptake by higher plant chloroplasts

Sugiura, M, Georgescu, M. N. and Takahashi, M. 2007

[学会発表] 光化学系IIにおける酸素発生の分子機能と構造:部位特異的変異を導入した好熱性シアノバクテリアが解明のブレークスルーになる

杉浦美羽 2007

[雑誌論文] Structural Perturbation of the Carboxylate Ligands to the Mn Cluster upon Ca^<2+>/Sr^<2+> Exchange in the S-state Cycle of Photosynthetic Oxygen

Suzuki, H., Taguchi, Y., Sugiura, M., Boussac, A., Noguchi, T 2006

[雑誌論文] Structural perturbation of the carboxylate ligands to the Mn cluster upon Ca2+/Sr2+ exchange in the S-state cycle of photosynthetic oxygen evolution as studied by flash-induced FTIR difference spectroscopy

Suzuki, H., Taguchi, Y., Sugiura, M., Boussac, A. and Noguchi, T. 2006

光合成を利用した酸素発生型光合成微生物による水素生産方法

光合成を利用した酸素発生型光合成微生物による水素生産方法