酵母を用いたL-アラビノースからエタノールへの高効率変換系の確立

トップ›研究者を探す›酵母を用いたL-アラビノースからエタノールへの高効率変換系の確立

酵母を用いたL-アラビノースからエタノールへの高効率変換系の確立

代表研究者	渡邉誠也
研究課題番号	KAKENHI-PROJECT-18760592
研究種目	若手研究(B)
研究分野	理工系工学プロセス工学生物機能・バイオプロセス
研究機関	京都大学
研究期間開始年月日	2006/4/1
研究期間終了年度	2008
研究ステータス	完了 (2008/4/1)
配分額（合計）	3,870,000 (直接経費 :3,600,000、間接経費 :270,000)
配分額（履歴）	2008年度：1,170,000 (直接経費 :900,000、間接経費 :270,000) 2007年度：1,300,000 (直接経費 :1,300,000) 2006年度：1,400,000 (直接経費 :1,400,000)
キーワード	バイオマスエタノールサッカロミセス酵母 L-アラビノース生物発酵

研究成果

[雑誌論文] Novel modified version of non-phosphorylated sugar metabolism: an alternative L-rhamnose pathway of Sphingomonas sp

Watanabe, S. and Makino, K 2009

[雑誌論文] Novel modified version of non-phosphorylated sugar metabolism : an alternative L-rhamnose pathway of Sphingomonas sp

Watanabe S. and Makino K 2009

[学会発表] Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase

Matsushika, A., Watanabe, S., Kodaki, T., Makino, K. and Sawayama, S 2008

[雑誌論文] Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism

Watanabe, S., Saimura, M. and Makino, K 2008

[雑誌論文] Metabolic fate of L-lactaldehyde derived from an alternative L-rhamnose pathway

Watanabe, S., Piyanart, S. and Makino, K. 2008

[雑誌論文] Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase

Matsushika, A., Watanabe, S., Kodaki, T., Makino, K. and Sawayama, S 2008

[雑誌論文] Gene cloning of cold-adapted isocitrate lyase from apsychrophilic bacterium, Cblwellia psychrerythraea, and analysis of amino acid residues involved in cold adaptation of this enzyme

Sato, Y., Watanabe, S., Yamaoka, N. and Takada, Y. 2008

[雑誌論文] Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase

Matsushika, A., Watanabe, S., Kodaki, T., Makino, K. and Sawayama, S. 2008

[雑誌論文] Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism

Watanabe, S., Saimura, M. and Makino, K. 2008

[学会発表] 細菌由来の新規なL-アラビノース代謝経路

渡邉誠也、牧野圭祐 2008

[雑誌論文] Expression of protein engineered NADP+-dependent xylitol dehydrogenase increases ethanol production from xylose in recombinant Saccharomyces cerevisiae

Matsushika, A., Watanabe, S., Kodaki, T., Makino, K., Inoue, H., Murakami, K. and Sawayama, S 2008

[雑誌論文] Expression of protein engineered NADP+-dependent xylito dehydrogenase increases ethanol production from xylose in recombinant Saccharomvces cerevisiae

Matsushika, A., Watanabe, S., Kodaki, T., Makino, K., Inoue, H., Murakami, K. and Sawayama, S. 2008

[雑誌論文] Gene cloning of cold-adapted isocitrate lyase from a psychrophilic bacterium, Colwellia psychrerythraea, and analysis of amino acid residues involved in cold adaptation of this enzyme

Sato, Y., Watanabe, S., Yamaoka, N. and Takada, Y 2008

[学会発表] 新規アラビノース代謝経路に関与するL-2-ケト-3-デオキシアラボネート脱水酵素の結晶構造解析

嶋田直子、三上文三、渡邉誠也、小瀧努、牧野圭祐 2007

[学会発表] 酵母の糖代謝経路の新展開〜バイオエタノール生産への活用から新規代謝経路の発見まで〜

渡邉誠也 2007

[雑誌論文] Protein engineering of xylose reductase from Pichia stipitis for improved NADH-specificity and the efficient ethanol production from xylose in recombinant Saccharomyces cerevisiae

Watanabe, S., Saleh, A.A., Pack, S.P., Annaluru, N., Kodaki, T. and Makino, K 2007

[学会発表] 脱水素酵素の補酵素特異性改変と安定化戦略

渡邉誠也、小瀧努、牧野圭祐 2007

[雑誌論文] 微生物の糖代謝経路に見られる新規な進化学的関係

渡邉誠也、牧野圭祐 2007

[雑誌論文] Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase involved in an alternative bacterial pathway of L-arabinose metabolism

Shimada, N., Mikami, B., Watanabe, S., Makino, K 2007

[学会発表] 新規アラビノース代謝経路に関与するL-2-ケト-3-デオキシアラボネート脱水酵素の結晶構造解析

嶋田直子、三上文三、渡邉誠也、小瀧努、牧野圭祐 2007

[学会発表] 改変型酵素を導入した酵母によるキシロースからのエタノール生産

松鹿昭則、渡邉誠也、小瀧努、牧野圭祐、井上宏之、村上克治、澤山茂樹 2007

[雑誌論文] Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP^+-dependent xylitol dehydrogenase.

Watanabe, S., Saleh, A. A., Pack, S. P., Annaluru, N., Kodaki, T. and Makino, K. 2007

[雑誌論文] Preliminary crystallographic analysis of L-2-keto-3-deoxyarabonate dehydratase involved in an alternative bacterial pathway of L-arabinose metabolism.

Shimada, N., Mikami, B., Watanabe, S., Makino, K. 2007

[雑誌論文] Protein engineering of xylose reductase from Pichia stipitis for improved NADH-specificity and the efficient ethanol production from xylose in recombinant Saccharomyces cerevisiae.

Watanabe, S., Saleh, A. A, Pack, S. P., Annaluru, N., Kodaki, T. and Makino, K. 2007

[雑誌論文] The Positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on the ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae.

Watanabe, S., Pack, S. P., Saleh, A. A., Pack, S. P., Annaluru, N., Kodaki, T. and Makino, K. 2007

[雑誌論文] タンパク質工学によるキシロース発酵酵母作出の新戦略

渡邉誠也、牧野圭祐 2007

[学会発表] Highly Efficient Bioethanol Fermentation from Woody Biomass

Watanabe, S., Kodaki, T. and Makino, K. 2007

[雑誌論文] Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc binding loop

Annaluru, N., Watanabe, S., Pack, S.P., Saleh, A.A., Kodaki, T., and Makino, K 2007

[雑誌論文] Efficient preparation of amine-modified oligodeoxynucleotide using modified H-phosphonate chemistry for DNA microarray fabrication

Kamisetty, N.K., Pack, S.P., Nonogawa, M., Devarayapalli, K.C., Watanabe, S., Kodaki, T. and Makino, K 2007

[雑誌論文] α-Ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate and hydroxy-L-proline : molecular and metabolic convergent evolution.

Watanabe, S., Yamada, M., Ohtsu, I., Makino, K. 2007

[雑誌論文] Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc binding loop.

Annaluru, N., Watanabe, S., Pack, S.P., Saleh, A.A., Kodaki, T., Makino, K. 2007

[雑誌論文] Thermostabilization of Pichia stipitis xylitol dehydrogenase by mutation of structural zinc binding loop.

Annaluru, N., Watanabe, S., Pack, S. P., Saleh, A. A., Kodaki, T., and Makino, K. 2007

[雑誌論文] The Positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on the ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae.

Watanabe, S., Pack, S.P., Saleh, A.A., Pack, S.P., Annaluru, N., Kodaki, T., Makino, K. 2007

[学会発表] Highly Efficient Bioethanol Fermentation from Woody Biomass

Watanabe, S., Kodaki, T. and Makino, K 2007

[雑誌論文] Base sequence- and Tm-dependent DNA oligomer separation by open tubular capillary columns carrying complementary DNA oligomers as probes

Devarayapalli, K.C., Pack, S.P., Kamisetty, N.K., Nonogawa, M., Watanabe, S., Kodaki, T. and Makino, K 2007

[雑誌論文] Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase

Watanabe, S., Saleh, A.A., Pack, S.P., Annaluru, N., Kodaki, T. and Makino, K 2007

[雑誌論文] The Positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on the ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae

Watanabe, S., Pack, S.P., Saleh, A.A., Pack, S.P., Annaluru, N., Kodaki, T. and Makino, K 2007

[雑誌論文] Identification and characterization of L-arabonate dehydratase, L-2-keto-3-deoxyarabonate dehydratase and L-arabinolactonase involved in an alternative pathway of L-arabinose metabolism : novel evolutionary insight into sugar metabolism

Watanabe, S., Shimada, N., Tajima, K., Kodaki, T. and Makino, K 2006

[学会発表] A novel bacterial L-arabinose pathway -cloning, expression and characterization of the metabolic genes-

Watanabe, S., Kodaki, T. and Makino, K 2006

[学会発表] Crystallization and preliminary X-ray diffraction studies of xylitol dehydrogenase, a key enzyme for bioethanol production using yeas

Matsui, J., Watanabe, S., Kodaki, T. and Makino, K 2006

[雑誌論文] Identification and characterization of L-arabonate dehydratase, L-2-keto-3-deoxyarabonate dehydratase and L-arabinolactonase involved in an alternative pathway of L-arabinose metabolism : novel evolutionary insight into sugar metabolism.

Watanabe, S., Shimada, N., Tajima, K., Kodaki, T., Makino, K. 2006

[学会発表] New approach for conversion of biomass to ethanol by recombinant Saccharomyces cerevisiae

Saleh, A. A., Watanabe, S., Annaluru, N., Kodaki, T. and Makino, K 2006

[雑誌論文] Cloning, expression and characterization of bacterial L-arabinose 1-dehydrogenase involved in an alternative pathway of L-arabinose metabolism

Watanabe, S., Kodaki, T. and Makino, K 2006

[雑誌論文] Construction of various mutants of xylose metabolizing enzymes for efficient conversion of biomass to ethanol

Saleh, A.A., Watanabe, S., Annaluru, N., Kodaki, T. and Makino, K 2006

[雑誌論文] Cloning, expression and characterization of bacterial L-arabinose l-dehydrogenase involved in an alternative pathway of L-arabinose metabolism.

Watanabe, S., Kodaki, T., Makino, K. 2006

[雑誌論文] Site-directed mutagenesis of a yeast gene for improvement of enzyme thermostability

Annaluru, N., Watanabe, S., Saleh, A.A., Kodaki, T., and Makino, K 2006

[学会発表] Construction of thermostable xylitol dehydrogenase, a key enzyme for efficient bioethanol production from xylose

Annaluru, N., Watanabe, S., Saleh, A. A., Kodaki, T. and Makino, K 2006

[学会発表] Improvement of thermostability of Pichia stipitis xylitol dehydrogenase by protein engineering

Annaluru, N., Watanabe, S., Saleh, A. A., Kodaki, T. and Makino, K 2006

[学会発表] A novel L-arabonate dehydratase involved in an alternative bacterial pathway of L-arabinose metabolism

Shimada, N., Watanabe, S., Shimada, N., Kodaki, T. and Makino, K 2006

[学会発表] A novel bacterial L-arabinose pathway: cloning, expression and characterization of the metabolic genes

Watanabe, S., Shimada, N., Kodaki, T. and Makino, K 2006

[学会発表] New approach for conversion of biomass to ethanol by recombinant Saccharomyces cerevisiae

Saleh, A. A., Watanabe, S., Annaluru, N., Kodaki, T. and Makino, K 2006

[図書] 化学フロンティア17 新エネルギー最前線-環境調和型エネルギーシステムの構築を目指して-

渡邉誠也, 小瀧努, 牧野圭祐 2006

[学会発表] Bioethanol from woody biomass by supercritical water technology -highly efficient ?fermentation process for bioethanol from woody biomass-

Makino, K., Saleh, A. A., Watanabe, S., Annaluru, N., and Kodaki, T 2006

[学会発表] Development of highly efficient fermentation process for bioethanol from biomass based on novel protein engineering approach

Watanabe, S., Saleh, A. A., Annaluru, N., Shimada, N., Kodaki, T. and Makino, K 2006

[学会発表] New approach for conversion of biomass to ethanol by recombinant Saccharomyces cerevisiae

Saleh, A. A., Watanabe, S., Annaluru, N., Kodaki, T. and Makino, K 2006

[雑誌論文] A novel α-ketoglutaric semialdehyde dehydrogenase : evolutionary insight into an alternative pathway of bacterial L-arabinose metabolism.

Watanabe, S., Kodaki, T., Makino, K. 2006

[雑誌論文] A novel α-ketoglutaric semialdehyde dehydrogenase : evolutionary insight into an alternative pathway of bacterial L-arabinose metabolism

Watanabe, S., Kodaki, T. and Makino, K 2006

[学会発表] Improvement of thermostability of Pichia stipitis xylitol dehydrogenase by protein engineering

Annaluru, N., Watanabe, S., Saleh, A. A., Kodaki, T. and Makino, K 2006

[雑誌論文] α-Ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate and hydroxy-L-proline : molecular and metabolic convergent evolution

Watanabe, S., Yamada, M., Ohtsu, I. and Makino, K

[雑誌論文] Metabolic fate of L-lactaldehyde derived from an alternative L-rhamnose pathway

Watanabe, S., Piyanart, S. and Makino, K