トップ›研究者を探す›次世代シーケンサーを活用した流域生物の全種網羅的な遺伝的多様性評価法の開発

次世代シーケンサーを活用した流域生物の全種網羅的な遺伝的多様性評価法の開発

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-16H04437
研究種目 基盤研究(B)
研究分野 理工系
工学
土木工学
土木環境システム
研究機関 愛媛大学
代表研究者 渡辺 幸三
研究分担者 加藤 幹男
研究分担者 八重樫 咲子
研究分担者 竹門 康弘
研究期間 開始年月日 2016/4/1
研究期間 終了年度 2018
研究ステータス 完了 (2018/4/1)
配分額（合計） 18,720,000 (直接経費 :14,400,000、間接経費 :4,320,000)
配分額（履歴） 2018年度：6,110,000 (直接経費 :4,700,000、間接経費 :1,410,000)
2017年度：5,850,000 (直接経費 :4,500,000、間接経費 :1,350,000)
2016年度：6,760,000 (直接経費 :5,200,000、間接経費 :1,560,000)
キーワード 遺伝的多様性
生息地分断化
次世代シークエンシング
流域
自然選択
生態系
次世代シーケンサー
次世代シークエンサー

研究成果

[学会発表] Sediment Microbial Diversity and Community Composition of Restored Gravel Bars in the Trinity River, California

Joeselle Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2019

[学会発表] Parallel genetic divergence of the coconut scale insect Aspidiotus rigidus (Hemiptera: Diaspididae) and its host-specific parasitoid Comperiella calauanica (Hymenoptera: Encyrtidae) in the Philippines

Naoto Ishitani, Joeselle Serrana, Thaddeus Carvajal, Billy Joel Almarinez, Alberto T. Barrion, Divina Amalin, Kozo Watanabe 2019

[学会発表] Detection of Wolbachia in Field-collected Dengue Vector Mosquito, Aedes aegypti in the Philippines

Kozo Watanabe 2019

[学会発表] Comparison of DNA metabarcoding and morphological identification for stream macroinvertebrate biodiversity assessment and monitoring

Joeselle Serrana, Yo Miyake, Maribet Gamboa, Kozo Watanabe 2019

[学会発表] 各種DNAマーカーを用いた水生昆虫の種判別

稲井公二、脇村圭、渡辺幸三、加藤幹男 2019

[学会発表] Protein quantitative traits loci (pQTL) expression of the stonefly Nemoura ovocercia (Plecoptera) along nationwide region of Japan

Maribet Gamboa, Maria Claret Tsuchiya, Shunsuke Yamano, Thaddeus Carvajal, Hisato Iwata, Kozo Watanabe 2019

[学会発表] Depression and Anxiety among peadiatric and adult in-patients with Dengue Fever in Metro Manila, Philippines

Von Ralph Dane M. HERBUELA, Ferdinand S. DE GUZMAN, Girly SOBREPE?A, Andrew Benedict F. CLAUDIO, Angelica Cecilia V. TOMAS, Carmina M. ARRIOLA-DELOS REYES, Rachele A. REGALADO, Mariama M. TEODORO, Kozo WATANABE 2019

[雑誌論文] Species Dispersal Mediates Opposing Influences of a Branching Network on Genetic Variation in a Metapopulation

Chiu, M-C, B. Li, K. Nukazawa, T. M. Carvajal, and K Watanabe 2019

[学会発表] Knowledge, attitude and practices on dengue fever among pediatric and adult in-patients in Metro Manila, Philippines

Von Ralph Dane M. HERBUELA, Ferdinand S. DE GUZMAN, Girly SOBREPE?A, Andrew Benedict F. CLAUDIO, Angelica Cecilia V. TOMAS, Carmina M. ARRIOLA-DELOS REYES, Rachele A. REGALADO, Mariama M. TEODORO, Kozo WATANABE 2019

[雑誌論文] Comparison of DNA Metabarcoding and Morphological Identification for Stream Macroinvertebrate Biodiversity Assessment and Monitoring

Serrana, J. M., Y. Miyake, M. Gamboa and K. Watanabe 2019

[雑誌論文] Genome-wide Signatures of Local Adaptation among Seven Stoneflies Species along a Nationwide Latitudinal Gradient in Japan

Gamboa, M. and K. Watanabe 2019

[学会発表] フィリピンのココヤシ寄生虫のDNA種分類及び集団遺伝解析

石谷直渡，Serrana, J. M.，Carvajal, T. M.， Almarinez, B. J. M.，Barrion, A. T.，Amalin, D. M.，渡辺幸三 2018

[学会発表] Ecological Influence of Sediment Bypass Tunnels on Macroinvertebrates in Dam-fragmented Rivers by DNA Metabarcoding

Joeselle SERRANA, Sakiko YAEGASHI, Shunsuke KONDOH, Bin LI , Christopher ROBINSON, and Kozo WATANABE 2018

[学会発表] DNA-based discrimination and population genetics of the coconut scale insect Aspidiotus rigidus Reyne in the Philippines

Naoto ISHITANI, Joeselle M. SERRANA, Thaddeus M. CARVAJAL, Billy Joel M. ALMARINEZ, Alberto T. BARRION, Divina M. AMALIN, Kozo WATANABE 2018

[学会発表] Genome-wide RAD-Seq Reveals Adaptive Divergence among Seven Stream Stoneflies along Nationwide Latitudinal Gradient in Japan

Maribet GAMBOA, Kozo WATANABE 2018

[学会発表] Molecular identification of the coconut scale insect species Aspidiotus rigidus Reyne and Aspidiotus destructor Signoret in the Philippines

Naoto Ishitani, Joeselle Serrana, Thaddeus Carvajal, Billy Joel Almarinez, Divina Amalin and Kozo Watanabe 2018

[学会発表] Using Next Generation Sequencing to Analyze Prey DNA in Faeces of An Avian Predator In Taiwan

Ming-Chih Chiu, Mei-Kwa Kuo, Kozo Watanabe 2018

[学会発表] Metabarcoding Based Macroinvertebrate Community Assessment Under the Influence of Artificial Gravel Bars in The Downstream of Dam in Trinity River

Bin Li, Joselle Serrana, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2018

[学会発表] Ecological Influence of Sediment Bypass Tunnels on Macroinvertebrates in Dam-fragmented Rivers by DNA Metabarcoding

Joeselle SERRANA, Sakiko YAEGASHI, Shunsuke KONDOH, Bin LI , Christopher ROBINSON, and Kozo WATANABE 2018

[学会発表] Assessment of The Ecological Influence of Sediment Bypass Tunnels On the Species Diversity of Macroinvertebrate Communities in Dam-Fragmented Rivers Using Dna Metabarcoding

Joeselle Serrana, Sakiko Yaegashi, Shunsuke Kondoh, Bin Li, Christopher T. Robinson and Kozo Watanabe 2018

[学会発表] Genetic Structure and Gene Flow Between Altitudinally Isolated Populations of Stenopsycheｍarmorata

Sakiko Yaegashi, Kozo Watanabe, Michael T. Monaghan, Tatsuo Omura 2018

[学会発表] Metabarcoding based macroinvertebrate community assessment under the influence of artificial gravel bars in the downstream of dam in Trinity River

Bin Li, Joselle Serrana, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2018

[学会発表] Phylogeography of Nemouridae family (Insecta, Plecoptera) in the Japanese Archipelago

Maribet Gamboa, David Muranyi, Shota Kanmori, Kozo Watanabe 2018

[学会発表] Genetic Analysis of Aedes aegypti in Manila, Philippines: Dispersal Pattern and Gene Expression Under Wolbachia Infection

Maria Angenica REGILME, Tatsuya INUKAI, Katherine VIACRUSIS, Divina AMALIN, Kozo WATANABE 2018

[学会発表] Assessment of The Ecological Influence of Sediment Bypass Tunnels On the Species Diversity of Macroinvertebrate Communities in Dam-Fragmented Rivers Using Dna Metabarcoding

Joeselle Serrana, Sakiko Yaegashi, Shunsuke Kondoh, Bin Li, Christopher T. Robinson and Kozo Watanabe 2018

[学会発表] Ecological Influence of Sediment Bypass Tunnels on Macroinvertebrates in Dam-fragmented Rivers by DNA Metabarcoding

Joeselle SERRANA, Sakiko YAEGASHI, Shunsuke KONDOH, Bin LI , Christopher ROBINSON, and Kozo WATANABE 2018

[学会発表] Dispersal variation of Aedes aegypti in Manila, Philippines - Do roads affect the population structure?

Regilme Maria Angenica, Inukai Tatsuya, Viacrusis Katherine, Amalin Divina, Watanabe Kozo 2018

[学会発表] Dengue forecasting models using climate variable as predictors at regional setting in Indonesia

Ruslan La Ane, Lia Farida, Thaddeus M. Carvajal, Kozo Watanabe 2018

[学会発表] Elucidating the Dispersion of Dengue Mosquito Vector, Ae. aegypti using Landscape Genetic Analysis

Thaddeus CARVAJAL, Kohei OGISHI, Sakiko YAEGESHI, Lara Fides HERNANDEZ, Katherine VIACRUSIS, Howell HO, Divina AMALIN, and Kozo WATANABE 2018

[学会発表] Fine-Spatial Genetic Analysis of Dengue Vector, Aedes aegypti, in Metropolitan Manila, Philippines

Thaddeus M. Carvajal, Kohei Ogishi, Sakiko Yaegeshi, Lara Fides T. Hernandez, Katherine M. Viacrusis, Howell T. Ho, Divina Amalin, and Kozo Watanabe 2018

[学会発表] Detecting Wolbachia Sp. in Dengue Mosquito Vector, Aedes aegypti and Its Potential as A Biological Mass Release Vector Control Program in The Philippines

Thaddeus M. Carvajal, Kazuki Hashimoto, Reza Kurniawan Harnandika, Divina Amalin, and Kozo Watanabe 2018

[学会発表] Ecological Influence of Sediment Bypass Tunnels on Macroinvertebrates in Dam-fragmented Rivers by DNA Metabarcoding

Joeselle SERRANA, Sakiko YAEGASHI, Shunsuke KONDOH, Bin LI , Christopher ROBINSON, and Kozo WATANABE 2018

[学会発表] Genome-wide RAD-seq revels adaptive divergence among seven stream stoneflies along a nationwide latitudinal gradient in Japan

Maribet Gamboa, Kozo Watanabe 2018

[学会発表] Wolbachia Detection and Distribution of Dengue Vector in Manila, Philippines

Tatsuya INUKAI, Maria Angenica Fulo REGILME, Thaddeus M. CARVAJAL, Billy Joel M. ALMARINEZ, Divina M. AMALIN, Kozo WATANABE 2018

[学会発表] デング熱媒介蚊における共生細菌ボルバキアの検出と遺伝構造解析

犬飼達也, Maria Angenica F. Regilme, Thaddeus M. Carvajal, Divina Amalin, Katherine M. Viacrusis, 渡辺幸三 2018

[学会発表] Dengue forecasting models using climate variable as predictors at regional setting in Indonesia

Ruslan La Ane, Lia Faridah, Thaddeus M. Carvajal, Kozo Watanabe 2018

[学会発表] マニラにおけるデング熱媒介蚊の生息分布と共生細菌ボルバキアの検出

犬飼達也，Maria Angenica F. Regilme, Thaddeus M. Carvajal，Divina Amalin，Katherine M. Viacrusis，渡辺幸三 2018

[学会発表] Genome-wide RAD-seq revels adaptive divergence among seven stream stoneflies along a nationwide latitudinal gradient in Japan

Maribet Gamboa, Kozo Watanabe 2018

[雑誌論文] Detection of Adaptive Divergence in Populations of the Stream Mayfly Ephemera strigata with Machine Learning

Li, B., S. Yaegashi, T. M. Carvajal, M. Gamboa and K. Watanabe 2018

[学会発表] Fine-Scale Genotyping and Dispersal Movement of Dengue Mosquito Vector, Aedes Aegypti in Metropolitan Mania

Thaddeus M. Carvajal, Kohei Ogishi, Sakiko Yaegeshi, Lara Fides T. Hernandez, Katherine M. Viacrusis, Howell T. Ho, Divina Amalin, and Kozo Watanabe 2018

[学会発表] Statistical forecasting of norovirus concentration in sewage as an indicator of future incidence

Fuminari Miura, Toru Watanabe, Kozo Watanabe, Akihiko Hata, Kensuke Fukushi 2018

[学会発表] フィリピンのココヤシ寄生虫と寄生バチの種間相互作用の遺伝学的解明

石谷直渡，Serrana, J. M.，Carvajal, T. M.， Almarinez, B. J. M.，Barrion, A. T.，Amalin, D. M.，渡辺幸三 2018

[学会発表] フィリピンのココヤシ寄生虫のDNA種分類及び集団遺伝解析

石谷直渡，Serrana, J. M.，Carvajal, T. M.， Almarinez, B. J. M.，Barrion, A. T.，Amalin, D. M.，渡辺幸三 2018

[学会発表] Ecological Influence of Sediment Bypass Tunnels on Macroinvertebrates in Dam-fragmented Rivers by DNA Metabarcoding

Joeselle SERRANA, Sakiko YAEGASHI, Shunsuke KONDOH, Bin LI , Christopher ROBINSON, and Kozo WATANABE 2018

[学会発表] Using Next Generation Sequencing to Analyze Prey DNA in Faeces of An Avian Predator In Taiwan

Ming-Chih Chiu, Mei-Kwa Kuo, Kozo Watanabe 2018

[学会発表] Comorbid Depression and Anxiety and other Psychiatric Symptoms among Dengue Patients in Metro Manila, Philippines

Von Ralph Dane M. HERBUELA, Ferdinand S. DE GUZMAN, Girly SOBREPE?A, Andrew Benedict F. CLAUDIO, Angelica Cecilia V. TOMAS, Carmina M. ARRIOLA-DELOS REYES, Kozo WATANABE 2018

[学会発表] Individual-Based Dengue Virus Surveillance in Mosquitoes Collected Concurrently with Suspected Patients: Implications On Spatial Transmission in Tarlac City, Philippines

Kozo Watanabe 2018

[学会発表] Metabarcoding based macroinvertebrate community assessment under the influence of artificial gravel bars in the downstream of dam in Trinity River

Bin Li, Joselle Serrana, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2018

[学会発表] Sediment microbial community composition and diversity of restored gravel bars in the Trinity River, California

Joeselle Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2018

[学会発表] DNA-based discrimination and population genetics of the coconut scale insect Aspidiotus rigidus Reyne in the Philippines

Naoto ISHITANI, Joeselle M. SERRANA, Thaddeus M. CARVAJAL, Billy Joel M. ALMARINEZ, Alberto T. BARRION, Divina M. AMALIN, Kozo WATANABE 2018

[学会発表] Genetic Structure and Gene Flow Between Altitudinally Isolated Populations of Stenopsycheｍarmorata

Sakiko Yaegashi, Kozo Watanabe, Michael T. Monaghan, Tatsuo Omura 2018

[学会発表] 水生昆虫の種判別におけるDNAマーカーの特徴

稲井公二，渡辺幸三，加藤幹男 2018

[学会発表] Metabarcoding Based Macroinvertebrate Community Assessment Under the Influence of Artificial Gravel Bars in The Downstream of Dam in Trinity River

Bin Li, Joselle Serrana, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe 2018

[学会発表] Genetic analysis of Aedes aegypti in Manila, Philippines: Dispersal pattern and gene expression under Wolbachia infection

Maria Angenica Regilme, Tatsuya Inukai, Katherine Viacrusis, Divina Amalin, and Kozo Watanabe 2018

[学会発表] Molecular identification of the coconut scale insect species Aspidiotus rigidus Reyne and Aspidiotus destructor Signoret in the Philippines

Naoto Ishitani, Joeselle Serrana, Thaddeus Carvajal, Billy Joel Almarinez, Divina Amalin and Kozo Watanabe 2018

[学会発表] Genome-wide RAD-Seq Reveals Adaptive Divergence among Seven Stream Stoneflies along Nationwide Latitudinal Gradient in Japan

Maribet GAMBOA, Kozo WATANABE 2018

[雑誌論文] Molecular phylogeny and diversification timing of the Nemouridae family (Insecta, Plecoptera) in the Japanese Archipelago

Gamboa, M., D. Muranyi, S. Kanmori, and K. Watanabe 2018

[学会発表] 流出解析を利用した河川無脊椎生物の生息分布予測モデルの開発

糠澤桂，風間聡，渡辺幸三 2017

[学会発表] 次世代シーケンサーで得られた水性昆虫DNA配列の種判別

稲井公二，渡辺幸三，加藤幹男 2017

[雑誌論文] 河川水中の環境DNAの次世代シーケンス解析を利用した水生昆虫の群集構造および生息個体数推定の可能性：従来型定量評価手法と比較して

八重樫咲子，細川大樹，渡辺幸三 2017

[学会発表] 河川水中の環境DNAの次世代シーケンス解析を利用した水生昆虫の群集構造および生息個体数推定の可能性：従来型定量評価手法と比較して

八重樫咲子，細川大樹，渡辺幸三 2017

[学会発表] Catchment-scaled species diversity modeling of stream invertebrates using a hydrological simulation

Nukazawa, K., Kazama, S., Watanabe, K., 2017

[学会発表] METABARCODING ANALYSIS OF WATER ENVIRONMENTAL DNA TO REVEAL SPECIES DIVERSITY OF AQUATIC INSECTS USING NEXT GENERATION SEQUENCING

Sakiko YAEGASHI, Kozo WATANABE 2017

[学会発表] メタバーコーディングを活用した排砂バイパスダム上下流間の河川底生動物の群集構造の評価

渡辺幸三，近藤俊介，泉昂佑，八重樫咲子 2016

[学会発表] 国際データべースに登録された水生昆虫DNAの整理と検証

稲井公二，渡辺幸三，加藤幹男 2016

[学会発表] 河川水中に存在するDNAを活用した重信川の流域内水生昆虫種多様性解析

八重樫咲子，渡辺幸三 2016

[学会発表] 次世代シーケンシング解析による排砂バイパス付きダム下流における水生昆虫群集の生物多様性解析

八重樫咲子，泉昴介，近藤俊介，渡辺幸三 2016

[学会発表] 日本の気候勾配に沿ったオナシカワゲラ（Nemoura.sp）のタンパク質発現パターンの多様性

山野俊介，Maribet Gamboa，渡辺幸三 2016

[学会発表] 生息場モデルを用いた河川健全度予測手法の開発

糠澤桂，風間聡，渡辺幸三 2016

[学会発表] 次世代シークエンシングを用いた河川昆虫カワゲラの日本列島の気候勾配に沿った発現変動遺伝子の探索

後藤友亮，Maribet Gamboa，渡辺幸三 2016

[学会発表] 次世代シーケンス解析による瀬切れ河川の水生昆虫複数種を対象とした流域内交流パターンの網羅的評価

八重樫咲子，泉昴佑，三宅洋，渡辺幸三 2016

[学会発表] 日本の気候勾配に沿ったオナシカワゲラ（Nemoura sp.）個体群の比較プロテオーム解析

山野俊介，Maribet Gamboa，Maria Claret Lauan，岩田久人，渡辺幸三 2016

[学会発表] 水文・生息場モデルを用いた河川健全度評価手法の提案

糠澤桂，風間聡，渡辺幸三 2016

[学会発表] Predicting Biodiversity of Aquatic Insects Under Changing Climates in Japan, and a Vision for Habitat Modeling of Dengue Vectors

Nukazawa, K., Kazama, S., Watanabe, K. 2016

[学会発表] Different ways of sperm transfer among the Capniidae (plecoptera), and its importance in the generic system of the family

D. Muranyi, W.H. Li, M. Gamboa, K. Watanabe 2016

[学会発表] Multispecies assessments of effects of habitat fragmentations on population genetic structures of stream invertebrates using next-generation sequencing

Sakiko YAEGASHI, Kozo WATANABE 2016

[学会発表] Gene-environmental association of stoneflies across environmental gradients in Japan

Maribet Gamboa, Kozo Watanabe 2016

[学会発表] Adaptive genetic consequences of climate change for stream insects: a hydrothermal simulation approach

Nukazawa, K., Kazama, S., Watanabe, K. 2016

[学会発表] 水生昆虫DNAによる種判別システムの構築

稲井公二，渡辺幸三，加藤幹男 2016

[雑誌論文] Characterization of Genes Coding for Histone H3, 18S rRNA, and Cytochrome oxidase subunit I of East Asian Mayflies (Ephemeroptera)

Kei Wakimura, Yasuhiro Takemon, Atsushi Takayanagi, Shin-ichi Ishiwata, Kozo Watanabe, Kazumi Tanida， Nobuyoshi Shimizu, and Mikio Kato 2016