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田中保

徳島大学

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リサーチマップ・
Jグローバル
KAKEN
注目研究

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2024年12月20日更新

職名: 教授
電話: 088-656-7256
電子メール: tanaka.tamotsu@tokushima-u.ac.jp
学歴: 1988/3: 徳島大学薬学部製薬学科卒業
1993/3: 徳島大学大学院薬学研究科博士課程修了
学位: 博士(薬学) (徳島大学) (1993年3月)
職歴・経歴: 1993/4: 福山大学助手，工学部食品工学科
1999/4: 福山大学講師，工学部応用生物科学科
2005/4: 福山大学助教授，生命工学部応用生物科学科
2005/9: Post doctoral Fellow at MD Anderson Cancer Center, the University of Texas
2006/9: 福山大学助教授，生命工学部応用生物科学科
2008/2: 徳島大学大学院准教授，ヘルスバイオサイエンス研究部(薬学系)
2019/4: 徳島大学大学院社会産業理工学研究部生物資源産業学部教授

専門分野・研究分野: 脂質生化学 (Lipid Biochemistry)

研究者総覧で最新データを確認する

2024年12月20日更新

専門分野・研究分野: 脂質生化学 (Lipid Biochemistry)
担当経験のある授業科目: ゲノム創薬特論 (大学院)
フードサイエンス (学部)
分子病態学 (学部)
卒業研究 (学部)
国際先端技術科学特論A (大学院)
国際先端技術科学特論B (大学院)
基礎生理学 (学部)
基礎食品化学 (学部)
生命科学史 (共通教育)
生物資源学研究 (大学院)
生物資源産業学B (学部)
生物資源産業学実習 (学部)
生物資源産業学専門英語 (学部)
生物資源産業学概論 (学部)
病態栄養学 (学部)
科学技術論B (大学院)
脂質生化学特論 (大学院)
英語論文講読 (学部)
英語論文講読Ⅰ (学部)
英語論文講読Ⅱ (学部)
食と健康概論 (学部)
食品・生物資源関連法規 (学部)
食品評価特論 (大学院)
食料生物科学特別実習 (大学院)
食料生物科学特別演習 (大学院)
食料生物科学特別研究 (大学院)
食料生物科学特別講義 (大学院)
食料科学基礎実習 (学部)
食料科学実習A (学部)
食料科学実習B (学部)
食料科学実習C (学部)
食料科学実習Ⅰ (学部)
食料科学実習Ⅱ (学部)
食料科学概論 (学部)
指導経験: 16人 (学士), 13人 (修士), 2人 (博士)

研究者総覧で最新データを確認する

2024年12月20日更新

専門分野・研究分野: 脂質生化学 (Lipid Biochemistry)

研究テーマ: 生理活性脂質に関する研究, 脂質代謝に関する研究

著書

Morito Katsuya, Ali Hanif, Kishino Shigenobu and Tamotsu Tanaka :
Fatty acid metabolism in peroxisomes and related disorders,
Springer, 2024. 森戸克弥, 田中保 :
3.6. 活性リン脂質 3.6.3. リゾホスファチジン酸およびホスファチジン酸,
朝倉書店, 2021年7月. 田中保 :
3.6. 活性リン脂質 3.6.2. スフィンゴリン脂質,
朝倉書店, 2021年7月. 田中保, 小暮健太朗 :
3.6. 活性リン脂質 3.6.1. はじめに,
朝倉書店, 2021年7月. 田中保, 森戸克弥 :
生物由来の油に関する文理融合型研究の推進ー食事あるいは腸内細菌に由来する非動物型脂肪酸の代謝ー,
2021年3月. Jun-Ichi Morishige, Ryouhei Yamashita, Tamotsu Tanaka and Kiyoshi Satouchi :
A Cleanup Method for Mass Spectrometric Analysis of Sphingosine- and Ceramide-1-Phosphate in Blood and Solid Tissue Using a Phosphate Capture Molecule.,
2017.

(要約): Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.
(キーワード): Ceramides / Humans / Lysophospholipids / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Sphingosine
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/7651_2017_6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28361484; ● Search Scopus @ Elsevier (PMID): 28361484; ● Search Scopus @ Elsevier (DOI): 10.1007/7651_2017_6

(DOI: 10.1007/7651_2017_6, PubMed: 28361484) J. Morishige, Tamotsu Tanaka and K. Satouchi :
A cleanup method for mass spectrometric analysis of sphingosine-1-phosphate in blood and solid tissues using a phosphate capture molecule,
Springer, May 2012.

(要約): Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate using a -phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples.
(キーワード): Animals / Cattle / Lung / Lysophospholipids / Phosphates / Rats / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Sphingosine
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/978-1-61779-800-9_4
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22528438; ● Search Scopus @ Elsevier (PMID): 22528438; ● Search Scopus @ Elsevier (DOI): 10.1007/978-1-61779-800-9_4

(DOI: 10.1007/978-1-61779-800-9_4, PubMed: 22528438) 田中保 :
ガスクロマトグラフィーによる分析,
丸善出版, 東京, 2011年12月. 盛重純一, 田中保, 里内清 :
リン酸モノエステル型リン脂質のMALDI-TOFMS,
丸善出版, 東京, 2011年11月. 田中保 :
アミノ酸他30項目,
丸善, 2005年. 田中保, 里内清 :
基礎生化学実験法脂質・糖質・複合糖質,
株式会社東京化学同人, 東京, 2000年12月. Tamotsu Tanaka, Toshinori Hattori, Maki Kouchi, Kaoru Hirano and Kiyoshi Satouchi :
Non-methylene interrupted polyenoic acid: Structural characterization and metabolism by fatty acid chain elongation system of rat liver,
American Oil Chemists' Society Press, 1998. 田中保, 里内清, 徳村彰 :
PAFと高度不飽和脂肪酸,
恒星社厚生閣, 1995年.

論文

Hanif ALi, Mone Yamanishi, Keigo Sunagawa, Mizuki Kumon, Rumana Yesmin Hasi, Mutsumi Aihara, Ryushi Kawakami and Tamotsu Tanaka :
Protective effect of oleic acid against very long-chain fatty acid-induced apoptosis in peroxisome-deficient CHO cells,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1869, No.3, 159452, 2024.

(要約): Very long-chain fatty acids (VLCFAs) are degraded exclusively in peroxisomes, as evidenced by the accumulation of VLCFAs in patients with certain peroxisomal disorders. Although accumulation of VLCFAs is considered to be associated with health issues, including neuronal degeneration, the mechanisms underlying VLCFAs-induced tissue degeneration remain unclear. Here, we report the toxic effect of VLCFA and protective effect of C18: 1 FA in peroxisome-deficient CHO cells. We examined the cytotoxicity of saturated and monounsaturated VLCFAs with chain-length at C20-C26, and found that longer and saturated VLCFA showed potent cytotoxicity at lower accumulation levels. Furthermore, the extent of VLCFA-induced toxicity was found to be associated with a decrease in cellular C18:1 FA levels. Notably, supplementation with C18:1 FA effectively rescued the cells from VLCFA-induced apoptosis without reducing the cellular VLCFAs levels, implying that peroxisome-deficient cells can survive in the presence of accumulated VLCFA, as long as the cells keep sufficient levels of cellular C18:1 FA. These results suggest a therapeutic potential of C18:1 FA in peroxisome disease and may provide new insights into the pharmacological effect of Lorenzo's oil, a 4:1 mixture of C18:1 and C22:1 FA.
(徳島大学機関リポジトリ): ● Metadata: 118916
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2024.159452
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38244676; ● Search Scopus @ Elsevier (PMID): 38244676; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2024.159452

(徳島大学機関リポジトリ: 118916, DOI: 10.1016/j.bbalip.2024.159452, PubMed: 38244676) Yoshimichi Takai, Rumana Yesmin Hasi, Naoko Matsumoto, Chiho Fujita, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Mutsumi Aihara, Toshiki Ishikawa, Hiroyuki Imai, Mayuko Wakida, Kazuya Ando and Tamotsu Tanaka :
Degradation of glycosylinositol phosphoceramide during plant tissue homogenization,
The Journal of Biochemistry, Vol.175, No.1, 115-124, 2024.

(要約): A convenient method for the determination of plant sphingolipids (glycosylinositol phosphoceramide, GIPC; glucosylceramide, GluCer; phytoceramide 1-phosphate, PC1P and phytoceramide, PCer) was developed. This method includes the extraction of lipids using 1-butanol, alkali hydrolysis with methylamine and separation by TLC. The amounts of sphingolipids in the sample were determined based on the relative intensities of standard sphingolipids visualized by primulin/UV on TLC. Using this method, we found that almost all GIPCs were degraded in response to tissue homogenization in cruciferous plants (cabbage, broccoli and Arabidopsis thaliana). The decrease in GIPCs was compensated for by increases in PC1P and PCer, indicating that GIPC was degraded by hydrolysis at the D and C positions of GIPC, respectively. In carrot roots and leaves, most of GIPC degradation was compensated for by an increase in PCer. In rice roots, the decrease in GIPCs was not fully explained by the increases in PC1P and PCer, indicating that enzymes other than phospholipase C and D activities operated. As the visualization of lipids on TLC is useful for detecting the appearance or disappearance of lipids, this method will be available for the characterization of metabolism of sphingolipids in plants.
(キーワード): Glycosphingolipids / Sphingolipids / Plants / Brassica / Arabidopsis
(徳島大学機関リポジトリ): ● Metadata: 118914
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvad080
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37827526; ● CiNii @ 国立情報学研究所 (CRID): 1520862036472955648; ● Summary page in Scopus @ Elsevier: 2-s2.0-85181760535

(徳島大学機関リポジトリ: 118914, DOI: 10.1093/jb/mvad080, PubMed: 37827526, CiNii: 1520862036472955648, Elsevier: Scopus) Jun-ichi Morishige, Kazuaki Yoshioka, Hiroki Nakata, Kazuhiro Ishimaru, Naoto Nagata, Tamotsu Tanaka, Yoh Takuwa and Hitoshi Ando :
Sphingosine kinase 1 is involved in triglyceride breakdown by maintaining lysosomal integrity in brown adipocytes,
Journal of Lipid Research, Vol.64, No.11, 100450, 2023.

(要約): Sphingosine 1-phosphate (S1P) has been implicated in brown adipose tissue (BAT) formation and energy consumption; however, the mechanistic role of sphingolipids, including S1P, in BAT remains unclear. Here, we showed that, in mice, BAT activation by cold exposure upregulated mRNA and protein expression of the S1P-synthesizing enzyme sphingosine kinase 1 (SphK1) and S1P production in BAT. Treatment of wild-type brown adipocytes with exogenous S1P or S1P receptor subtype-selective agonists stimulated triglyceride (TG) breakdown only marginally, compared with noradrenaline. However, genetic deletion of Sphk1 resulted in hypothermia and diminished body weight loss upon cold exposure, suggesting that SphK1 is involved in thermogenesis through mechanisms different from receptor-mediated, extracellular action of S1P. In BAT of wild-type mice, SphK1 was localized largely in the lysosomes of brown adipocytes. In the brown adipocytes of Sphk1 mice, the number of lysosomes was reduced and lysosomal function, including proteolytic activity, acid esterase activity, and motility, was impaired. Concordantly, nuclear translocation of transcription factor EB, a master transcriptional regulator of lysosome biogenesis, was reduced, leading to decreased mRNA expression of the lysosome-related genes in Sphk1 BAT. Moreover, BAT of Sphk1 mice showed greater TG accumulation with dominant larger lipid droplets in brown adipocytes. Inhibition of lysosomes with chloroquine resulted in a less extent of triglyceride accumulation in Sphk1 brown adipocytes compared with wild-type brown adipocytes, suggesting a reduced lysosome-mediated TG breakdown in Sphk1 mice. Our results indicate a novel role of SphK1 in lysosomal integrity, which is required for TG breakdown and thermogenesis in BAT.
(キーワード): Mice / Animals / Adipocytes, Brown / Signal Transduction / Phosphotransferases (Alcohol Group Acceptor) / Sphingosine / Adipose Tissue, Brown / RNA, Messenger / Lysophospholipids / Triglycerides
(徳島大学機関リポジトリ): ● Metadata: 119223
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jlr.2023.100450
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37751791; ● Search Scopus @ Elsevier (PMID): 37751791; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jlr.2023.100450

(徳島大学機関リポジトリ: 119223, DOI: 10.1016/j.jlr.2023.100450, PubMed: 37751791) Keisuke Kitakaza, Hanif Ali, Raiki Kimoto, Yasuhiro Takenouchi, Hironobu Ishimaru, Atsushi Yamashita, Natsuo Ueda, Tamotsu Tanaka, Yasuo Okamoto and Kazuhito Tsuboi :
GDE7 produces cyclic phsphpatidic acid in the ER lumen functioning as a lysophospholipid mediator,
Communications Biology, Vol.6, No.1, 524, 2023.

(要約): Cyclic phosphatidic acid (cPA) is a lipid mediator, which regulates adipogenic differentiation and glucose homeostasis by suppressing nuclear peroxisome proliferator-activated receptor γ (PPARγ). Glycerophosphodiesterase 7 (GDE7) is a Ca-dependent lysophospholipase D that localizes in the endoplasmic reticulum. Although mouse GDE7 catalyzes cPA production in a cell-free system, it is unknown whether GDE7 generates cPA in living cells. Here, we demonstrate that human GDE7 possesses cPA-producing activity in living cells as well as in a cell-free system. Furthermore, the active site of human GDE7 is directed towards the luminal side of the endoplasmic reticulum. Mutagenesis revealed that amino acid residues F227 and Y238 are important for catalytic activity. GDE7 suppresses the PPARγ pathway in human mammary MCF-7 and mouse preadipocyte 3T3-L1 cells, suggesting that cPA functions as an intracellular lipid mediator. These findings lead to a better understanding of the biological role of GDE7 and its product, cPA.
(キーワード): Mice / Animals / Humans / Phosphatidic Acids / PPAR gamma / Lysophospholipids / Endoplasmic Reticulum / Phosphoric Diester Hydrolases
(徳島大学機関リポジトリ): ● Metadata: 118893
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s42003-023-04900-4
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37193762; ● Search Scopus @ Elsevier (PMID): 37193762; ● Search Scopus @ Elsevier (DOI): 10.1038/s42003-023-04900-4

(徳島大学機関リポジトリ: 118893, DOI: 10.1038/s42003-023-04900-4, PubMed: 37193762) Morito Katsuya, Shimizu Ryota, Ali Hanif, Shimada Akina, Miyazaki Tohru, Takahashi Naoko, Rahman Motiur M., Tsuji Kazuki, Shimozawa Nobuyuki, Michiyasu Nakao, Shigeki Sano, Momoyo Azuma, Nanjundan Meera, Kentaro Kogure and Tamotsu Tanaka :
Molecular species profiles of plasma ceramides in different clinical types of X-linked adrenoleukodystrophy,
The Journal of Medical Investigation : JMI, Vol.70, No.3.4, 403-410, 2023.

(要約): X-linked adrenoleukodystrophy (X-ALD) is a genetic disorder associated with peroxisomal dysfunction. Patients with this rare disease accumulate very long-chain fatty acids (VLCFAs) in their bodies because of impairment of peroxisomal VLCFA ?-oxidation. Several clinical types of X-ALD, ranging from mild (axonopathy in the spinal cord) to severe (cerebral demyelination), are known. However, the molecular basis for this phenotypic variability remains largely unknown. In this study, we determined plasma ceramide (CER) profile using liquid chromatography-tandem mass spectrometry. We characterized the molecular species profile of CER in the plasma of patients with mild (adrenomyeloneuropathy;AMN) and severe (cerebral) X-ALD. Eleven X-ALD patients (five cerebral, five AMN, and one carrier) and 10 healthy volunteers participated in this study. Elevation of C26:0 CER was found to be a common feature regardless of the clinical types. The level of C26:1 CER was significantly higher in AMN but not in cerebral type, than that in healthy controls. The C26:1 CER level in the cerebral type was significantly lower than that in the AMN type. These results suggest that a high level of C26:0 CER, along with a control level of C26:1 CER, is a characteristic feature of the cerebral type X-ALD. J. Med. Invest. 70 : 403-410, August, 2023.
(キーワード): Humans / Adrenoleukodystrophy / Ceramides
(徳島大学機関リポジトリ): ● Metadata: 118324
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.70.403
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37940524; ● Search Scopus @ Elsevier (PMID): 37940524; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.70.403

(徳島大学機関リポジトリ: 118324, DOI: 10.2152/jmi.70.403, PubMed: 37940524) Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Peroxisomes attenuate cytotoxicity of very long-chain fatty acids,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1868, No.2, 159259, 2023.

(要約): One of the major functions of peroxisomes in mammals is oxidation of very long-chain fatty acids (VLCFAs). Genetic defects in peroxisomal β-oxidation result in the accumulation of VLCFAs and lead to a variety of health problems, such as demyelination of nervous tissues. However, the mechanisms by which VLCFAs cause tissue degeneration have not been fully elucidated. Recently, we found that the addition of small amounts of isopropanol can enhance the solubility of saturated VLCFAs in an aqueous medium. In this study, we characterized the biological effect of extracellular VLCFAs in peroxisome-deficient Chinese hamster ovary (CHO) cells, neural crest-derived pheochromocytoma cells (PC12), and immortalized adult Fischer rat Schwann cells (IFRS1) using this solubilizing technique. C20:0 FA was the most toxic of the C16-C26 FAs tested in all cells. The basis of the toxicity of C20:0 FA was apoptosis and was observed at 5 μM and 30 μM in peroxisome-deficient and wild-type CHO cells, respectively. The sensitivity of wild-type CHO cells to cytotoxic C20:0 FA was enhanced in the presence of a peroxisomal β-oxidation inhibitor. Further, a positive correlation was evident between cell toxicity and the extent of intracellular accumulation of toxic FA. These results suggest that peroxisomes are pivotal in the detoxification of apoptotic VLCFAs by preventing their accumulation.
(キーワード): Cricetinae / Animals / Peroxisomes / Fatty Acids / CHO Cells / Cricetulus / Oxidation-Reduction
(徳島大学機関リポジトリ): ● Metadata: 117825
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2022.159259
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36460260; ● Search Scopus @ Elsevier (PMID): 36460260; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2022.159259

(徳島大学機関リポジトリ: 117825, DOI: 10.1016/j.bbalip.2022.159259, PubMed: 36460260) Rumana Yesmin Hasi, Toshiki Ishikawa, Keigo Sunagawa, Yoshimichi Takai, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Keizo Yuasa, Mutsumi Aihara, Kaori Kanemaru, Hiroyuki Imai and Tamotsu Tanaka :
Nonspecific phospholipase C3 of radish has phospholipase D activity toward glycosylinositol phosphoceramide,
FEBS Letters, Vol.596, No.23, 3024-3036, 2022.

(要約): Glycosylinositol phosphoceramide (GIPC) is a major sphingolipid in the plasma membranes of plants. Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of the D position of GIPC in cabbage and named this activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as a PLD. The recombinant NPC3 protein obtained by heterologous expression system in Escherichia coli produced PC1P from GIPC and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish and Arabidopsis thaliana. From these results, we conclude that NPC3 is one of the enzymes that degrade GIPC.
(キーワード): Phospholipase D / Raphanus / Phospholipases / Sphingolipids / Brassica / Arabidopsis
(徳島大学機関リポジトリ): ● Metadata: 117824
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/1873-3468.14520
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36266963; ● Search Scopus @ Elsevier (PMID): 36266963; ● Search Scopus @ Elsevier (DOI): 10.1002/1873-3468.14520

(徳島大学機関リポジトリ: 117824, DOI: 10.1002/1873-3468.14520, PubMed: 36266963) S. Khaledur M. Rahman, Zahir Hussain, Katsuya Morito, Naoko Takahashi, Mohammad Mamun Sikder, Tamotsu Tanaka, Ken-ichi Ohta, Masaki Ueno, Hiroo Takahashi, Tohru Yamamoto, Makoto Murakami, Toru Uyama and Natsuo Ueda :
Formation of N-acyl-phosphatidylethanolamines by cytosolic phospholipase A₂ϵ in an ex vivo murine model of brain ischemia,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 159222, 2022.

(要約): N-Acyl-phosphatidylethanolamines (NAPEs), a minor class of membrane glycerophospholipids, accumulate along with their bioactive metabolites, N-acylethanolamines (NAEs) during ischemia. NAPEs can be formed through N-acylation of phosphatidylethanolamine by cytosolic phospholipase Aϵ (cPLAϵ, also known as PLA2G4E) or members of the phospholipase A and acyltransferase (PLAAT) family. However, the enzyme responsible for the NAPE production in brain ischemia has not yet been clarified. Here, we investigated a possible role of cPLAϵ using cPLAϵ-deficient (Pla2g4e) mice. As analyzed with brain homogenates of wild-type mice, the age dependency of Ca-dependent NAPE-forming activity showed a bell-shape pattern being the highest at the first week of postnatal life, and the activity was completely abolished in Pla2g4e mice. However, liquid chromatography-tandem mass spectrometry revealed that the NAPE levels of normal brain were similar between wild-type and Pla2g4e mice. In contrast, post-mortal accumulations of NAPEs and most species of NAEs were only observed in decapitated brains of wild-type mice. These results suggested that cPLAϵ is responsible for Ca-dependent formation of NAPEs in the brain as well as the accumulation of NAPEs and NAEs during ischemia, while other enzyme(s) appeared to be involved in the maintenance of basal NAPE levels.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2022.159222
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35988872; ● Search Scopus @ Elsevier (PMID): 35988872; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2022.159222

(DOI: 10.1016/j.bbalip.2022.159222, PubMed: 35988872) Ali Hanif, Morito Katsuya, Rumana Hasi Yesmin, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Sango Kazunori and Tamotsu Tanaka :
Characterization of uptake and metabolism of very long-chain fatty acids in peroxisome-deficient CHO cells,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1867, No.2, 159088, 2022.

(要約): Fatty acids (FAs) longer than C20 are classified as very long-chain fatty acids (VLCFAs). Although biosynthesis and degradation of VLCFAs are important for the development and integrity of the myelin sheath, knowledge on the incorporation of extracellular VLCFAs into the cells is limited due to the experimental difficulty of solubilizing them. In this study, we found that a small amount of isopropanol solubilized VLCFAs in aqueous medium by facilitating the formation of the VLCFA/albumin complex. Using this solubilizing technique, we examined the role of the peroxisome in the uptake and metabolism of VLCFAs in Chinese hamster ovary (CHO) cells. When wild-type CHO cells were incubated with saturated VLCFAs (S-VLCFAs), such as C23:0 FA, C24:0 FA, and C26:0 FA, extensive uptake was observed. Most of the incorporated S-VLCFAs were oxidatively degraded without acylation into cellular lipids. In contrast, in peroxisome-deficient CHO cells uptake of S-VLCFAs was marginal and oxidative metabolism was not observed. Extensive uptake and acylation of monounsaturated (MU)-VLCFAs, such as C24:1 FA and C22:1 FA, were observed in both types of CHO cells. However, oxidative metabolism was evident only in wild-type cells. Similar manners of uptake and metabolism of S-VLCFAs and MU-VLCFAs were observed in IFRS1, a Schwan cell-derived cell line. These results indicate that peroxisome-deficient cells limit intracellular S-VLCFAs at a low level by halting uptake, and as a result, peroxisome-deficient cells almost completely lose the clearance ability of S-VLCFAs accumulated outside of the cells.
(徳島大学機関リポジトリ): ● Metadata: 116579
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2021.159088
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34848380; ● CiNii @ 国立情報学研究所 (CRID): 1360013168847983360; ● Search Scopus @ Elsevier (PMID): 34848380; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2021.159088

(徳島大学機関リポジトリ: 116579, DOI: 10.1016/j.bbalip.2021.159088, PubMed: 34848380, CiNii: 1360013168847983360) Tamotsu Tanaka, Kazuya Koyama, Naoko Takahashi, Katsuya Morito, Hanif Ali, Momoyo Azuma, Kozo Kagawa, Hiroshi Kawano, Rumana Yesmin, Mutsumi Aihara and Yasuhiko Nishioka :
Lysophosphatidic acid, ceramide 1-phosphate and sphingosine 1-phosphate in peripheral blood of patients with idiophathic pulmonary fibrosis,
The Journal of Medical Investigation : JMI, Vol.69, No.3.4, 196-203, 2022.

(要約): Idiopathic pulmonary fibrosis (IPF) is the most common idiopathic interstitial pneumonias. Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are signaling lipids that evoke growth factor-like responses to many cells. Recent studies revealed the involvement of LPA and S1P in the pathology of IPF. In this study, we determined LPA, S1P and ceramide 1-phosphate (C1P) in peripheral blood plasma of IPF patients, and examined correlation to the vital capacity of lung (VC), an indicator of development of fibrosis. Blood plasma samples were taken from eleven patients with IPF and seven healthy volunteers. The lipids of the sample were extracted and subjected to liquid chromatography-tandem mass spectrometry for analysis. Results showed that there is a significant negative correlation between VC and plasma LPA levels, indicating that IPF patients with advanced fibrosis had higher concentration of LPA in their plasma. Average of S1P levels were significantly higher in IPF patients than those in healthy subjects. Although it is not statistically significant, a similar correlation trend that observed in LPA levels also found between VC and S1P levels. These results indicated that plasma LPA and S1P may be associated with deterioration of pulmonary function of IPF patients. J. Med. Invest. 69 : 196-203, August, 2022.
(キーワード): Ceramides / Fibrosis / Humans / Idiopathic Pulmonary Fibrosis / Lysophospholipids / Sphingosine
(徳島大学機関リポジトリ): ● Metadata: 117621
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.69.196
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36244770; ● Search Scopus @ Elsevier (PMID): 36244770; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.69.196

(徳島大学機関リポジトリ: 117621, DOI: 10.2152/jmi.69.196, PubMed: 36244770) Kazuhito Tsuboi, Tatsuya Tai, Ryouhei Yamashita, Hanif Ali, Takashi Watanabe, Toru Uyama, Yoko Okamoto, Keisuke Kitakaze, Yasuhiro Takenouchi, Shinji Go, Iffat Sonia Ara Rahman, Hitoshi Houchi, Tamotsu Tanaka, Yasuo Okamoto, Akira Tokumura, Junko Matsuda and Natsuo Ueda :
Involvement of acid ceramidase in the degradation of bioactive N-acylethanolamines,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1866, No.9, 158972, 2021.

(要約): Bioactive N-acylethanolamines (NAEs) include palmitoylethanolamide, oleoylethanolamide, and anandamide, which exert anti-inflammatory, anorexic, and cannabimimetic actions, respectively. The degradation of NAEs has been attributed to two hydrolases, fatty acid amide hydrolase and NAE acid amidase (NAAA). Acid ceramidase (AC) is a lysosomal enzyme that hydrolyzes ceramide (N-acylsphingosine), which resembles NAAA in structure and function. In the present study, we examined the role of AC in the degradation of NAEs. First, we demonstrated that purified recombinant human AC can hydrolyze various NAEs with lauroylethanolamide (C12:0-NAE) as the most reactive NAE substrate. We then used HEK293 cells metabolically labeled with [C]ethanolamine, and revealed that overexpressed AC lowered the levels of C-labeled NAE. As analyzed with liquid chromatography-tandem mass spectrometry, AC overexpression decreased the amounts of different NAE species. Furthermore, suppression of endogenous AC in LNCaP prostate cells by siRNA increased the levels of various NAEs. Lastly, tissue homogenates from mice genetically lacking saposin D, a presumable activator protein of AC, showed much lower hydrolyzing activity for NAE as well as ceramide than the homogenates from wild-type mice. These results demonstrate the ability of AC to hydrolyze NAEs and suggest its physiological role as a third NAE hydrolase.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2021.158972
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34033896; ● Search Scopus @ Elsevier (PMID): 34033896; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2021.158972

(DOI: 10.1016/j.bbalip.2021.158972, PubMed: 34033896) Hanif Ali, Ryouhei Yamashita, Jun-ichi Morishige, Katsuya Morito, Naoya Kakiuchi, Junji Hayashi, Mutsumi Aihara, Ryushi Kawakami, Koichiro Tsuchiya and Tamotsu Tanaka :
Massspectrometric analysis of sphingomyelin with N-alfa-hydroxy fatty acyl residue in mouse tissues,
Lipids, Vol.56, No.2, 181-188, 2021.

(徳島大学機関リポジトリ): ● Metadata: 117826
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/lipd.12285
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1002/lipd.12285

(徳島大学機関リポジトリ: 117826, DOI: 10.1002/lipd.12285) Midori Fukui, Toshihiko Tsutsumi, Aimi Yamamoto-Mikami, Katsuya Morito, Naoko Takahashi, Tamotsu Tanaka, Tekeshi Iwasa, Akira Kuwahara, Minoru Irahara and Akira Tokumura :
Distinct contributions of two choline-producing enzymatic activities to lysophosphatidic acid production in human amniotic fluid from pregnant women in the second trimester and after parturition,
Prostaglandins & Other Lipid Mediators, Vol.150, 106471, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.prostaglandins.2020.106471
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32585250; ● Summary page in Scopus @ Elsevier: 2-s2.0-85087143410

(DOI: 10.1016/j.prostaglandins.2020.106471, PubMed: 32585250, Elsevier: Scopus) Toshihiko Tsutsumi, Risa Matsuda, Katsuya Morito, Kohei Kawabata, Miho Yokota, Miki Nikawadori, Manami Inoue-Fujiwara, Satoshi Kawashima, Mayumi Hidaka, Takenori Yamamoto, Naoshi Yamazaki, Tamotsu Tanaka, Yasuo Shinohara, Hiroyuki Nishi and Akira Tokumura :
Identification of human glycerophosphodiesterase 3 as an ectophospholipase C that converts the G protein-coupled receptor 55 agonist lysophosphatidylinositol to bioactive monoacylglycerols in cultured mammalian cells.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1865, No.9, 158761, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2020.158761
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2020.158761

(DOI: 10.1016/j.bbalip.2020.158761) Rumana Yesmin Hasi, Dai Majima, Katsuya Morito, Hanif Ali, Kentaro Kogure, Meera Nanjundan, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities.,
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, Vol.1152, 122213, 2020.

(要約): Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50-70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids.
(徳島大学機関リポジトリ): ● Metadata: 115195
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jchromb.2020.122213
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32615533; ● Search Scopus @ Elsevier (PMID): 32615533; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jchromb.2020.122213

(徳島大学機関リポジトリ: 115195, DOI: 10.1016/j.jchromb.2020.122213, PubMed: 32615533) Tohru Hashimura, Jun-ichi Kido, Risa Matsuda, Miho Yokota, Hirokazu Matsui, Manami Inoue-Fujiwara, Yuji Inagaki, Mayumi Hidaka, Tamotsu Tanaka, Toshihiko Tsutsumi, Toshihiko Nagata and Akira Tokumura :
A low level of lysophosphatidic acid in human gingival crevicular fluid from patients with periodontitis due to high soluble lysophospholipase activity: Its potential protective role on alveolar bone loss by periodontitis.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1865, No.7, 158698, 2020.

(要約): We previously detected a submicromolar concentration of lysophosphatidic acid (LPA) in human saliva. Here, we compare LPA concentrations in human gingival crevicular fluid (GCF) from patients with periodontitis and healthy controls, and examine how the local LPA levels are regulated enzymatically. The concentrations of LPA and its precursor lysophospholipids in GCF was measured by liquid chromatography-tandem mass spectrometry. The LPA-producing and LPA-degrading enzymatic activities were measured by quantifying the liberated choline and free fatty acid, respectively. The concentration of LPA in GCF of periodontitis patients was lower than that of healthy controls, due to higher soluble lysophospholipase activity toward LPA. LPA was found to prevent survival of Sa3, a human gingival epithelium-derived tumor cell line, activate Sa3 through Ca mobilization, and release interleukin 6 from Sa3 in vitro. Furthermore, local injection of LPA into the gingiva attenuated ligature-induced experimental alveolar bone loss induced by oral bacteria inoculation in a rat model of periodontitis in vivo. A high concentration of LPA in human GCF is necessary to maintain normal gingival epithelial integrity and function, protecting the progression of periodontitis.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2020.158698
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32179099; ● Search Scopus @ Elsevier (PMID): 32179099; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2020.158698

(DOI: 10.1016/j.bbalip.2020.158698, PubMed: 32179099) Tasuku Torao, Miyuki Mimura, Yasufumi Ohshima, Kohki Fujikawa, Mahadi Hasan, Tatsuharu Shimokawa, Naoshi Yamazaki, Hidenori ANDO, Tatsuhiro Ishida, Tatsuya Fukuta, Tamotsu Tanaka and Kentaro Kogure :
Characteristics of unique endocytosis induced by weak current for cytoplasmic drug delivery,
International Journal of Pharmaceutics, Vol.576, 119010, 2020.

(徳島大学機関リポジトリ): ● Metadata: 114728
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijpharm.2019.119010
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijpharm.2019.119010

(徳島大学機関リポジトリ: 114728, DOI: 10.1016/j.ijpharm.2019.119010) Smriti Binte Sultana Mustafiz, Toru Uyama, Katsuya Morito, Naoko Takahashi, Katsuhisa Kawai, Zahir Hussain, Kazuhito Tsuboi, Nobukazu Araki, Kei Yamamoto, Tamotsu Tanaka and Natsuo Ueda :
Intracellular Ca²⁺-dependent formation of N-acyl-phosphatidylethanolamines by human cytosolic phospholipase A₂ϵ.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1864, No.12, 158515, 2019.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2019.158515
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85071868836

(DOI: 10.1016/j.bbalip.2019.158515, Elsevier: Scopus) Katsuya Morito, Ryota Shimizu, Nahoko Kitamura, Si-Bum Park, Shigenobu Kishino, Jun Ogawa, Tatsuya Fukuta, Kentaro Kogure and Tamotsu Tanaka :
Gut microbial metabolites of linoleic acid are metabolized by accelerated peroxisomal β-oxidation in mammalian cells,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1864, No.11, 1619-1628, 2019.

(要約): Microorganisms in animal gut produce unusual fatty acids from the ingested diet. Two types of hydroxy fatty acids (HFAs), 10-hydroxy-cis-12-octadecenoic acid (HYA) and 10-hydroxy-octadecanoic acid (HYB), are linoleic acid (LA) metabolites produced by Lactobacillus plantarum. In this study, we investigated the metabolism of these HFAs in mammalian cells. When Chinese hamster ovary (CHO) cells were cultured with HYA, approximately 50% of the supplemented HYA disappeared from the dish within 24 h. On the other hand, the amount of HYA that disappeared from the dish of peroxisome (PEX)-deficient CHO cells was lower than 20%. Significant amounts of C2- and C4-chain-shortened metabolites of HYA were detected in culture medium of HYA-supplemented CHO cells, but not in medium of PEX-deficient cells. These results suggested that peroxisomal β-oxidation is involved in the disappearance of HYA. The PEX-dependent disappearance was observed in the experiment with HYB, but not with LA. We also found that HYA treatment up-regulates peroxisomal β-oxidation activity of human gastric MKN74 cells and intestinal Caco-2 cells. These results indicate a possibility that HFAs produced from gut bacteria affect lipid metabolism of host via modulation of peroxisomal β-oxidation activity.
(徳島大学機関リポジトリ): ● Metadata: 113682
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2019.07.010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31351225; ● Search Scopus @ Elsevier (PMID): 31351225; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2019.07.010

(徳島大学機関リポジトリ: 113682, DOI: 10.1016/j.bbalip.2019.07.010, PubMed: 31351225) Rumana Yesmin Hasi, Makoto Miyagi, Takashi Kida, Tatsuya Fukuta, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Quantitative Analysis of Glycosylinositol Phosphoceramide and Phytoceramide 1-Phosphate in Vegetables,
Journal of Nutritional Science and Vitaminology, Vol.65, No.Supplement, S175-S179, 2019.

(要約): Previously, we found an unidentified sphingolipid in cabbage, and determined it as phytoceramide 1-phosphate (PC1P). PC1P is found to be produced from glycosylinositol phosphoceramide (GIPC) by the action of phospholipase D (PLD) activity. Although GIPC is abundant sphingolipid, especially in cruciferous vegetables, amount of daily intake, digestibility and nutritional activity of GIPC are not well understood. Here, we investigated amounts of GIPC and PC1P in vegetables. GIPC was found in all vegetables examined (13 kinds) at levels 3-20 mg/100 g (wet weight). On the other hand, PC1P was present in limited vegetables which show higher GIPC-PLD activity, such as inner cabbage leaves (5.2 mg/100 g). Because PC1P is formed during homogenization by activated GIPC-PLD, level of PC1P in boiled cabbage leaves was very low. Although digestibility of GIPC is unknown at present, a portion of dietary GIPC is considered to be converted to PC1P during mastication by plant-derived GIPC-PLD activity in some vegetables.
(キーワード): Brassica / Ceramides / Glycosphingolipids / Inositol / Phosphates / Phospholipase D / Plant Leaves / Sphingolipids / Vegetables
(徳島大学機関リポジトリ): ● Metadata: 115197
(出版サイトへのリンク): ● Publication site (DOI): 10.3177/jnsv.65.S175
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31619623; ● Summary page in Scopus @ Elsevier: 2-s2.0-85073476996

(徳島大学機関リポジトリ: 115197, DOI: 10.3177/jnsv.65.S175, PubMed: 31619623, Elsevier: Scopus) Hasi Yesmin Rumana, Makoto Miyagi, Katsuya Morito, Toshiki Ishikawa, Maki Kawai-Yamada, Hiroyuki Imai, Tatsuya Fukuta, Kentaro Kogure, Kaori Kanemaru, Junji Hayashi, Ryushi Kawakami and Tamotsu Tanaka :
Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation,
The Journal of Biochemistry, Vol.166, No.5, 441-448, 2019.

(要約): Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipid in plants and fungi. Recently, we detected GIPC-specific phospholipase D (GIPC-PLD) activity in plants. Here, we found that GIPC-PLD activity in young cabbage leaves catalyzes transphosphatidylation. The available alcohol for this reaction is a primary alcohol with a chain length below C4. Neither secondary alcohol, tertiary alcohol, choline, serine nor glycerol serves as an acceptor for transphosphatidylation of GIPC-PLD. We also found that cabbage GIPC-PLD prefers GIPC containing two sugars. Neither inositol phosphoceramide, mannosylinositol phosphoceramide nor GIPC with three sugar chains served as substrate. GIPC-PLD will become a useful catalyst for modification of polar head group of sphingophospholipid.
(キーワード): Biocatalysis / Brassica / Ceramides / Inositol / Molecular Structure / Phosphatidylcholines / Phospholipase D / Plant Leaves
(徳島大学機関リポジトリ): ● Metadata: 113685
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvz056
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31504617; ● Summary page in Scopus @ Elsevier: 2-s2.0-85073764089

(徳島大学機関リポジトリ: 113685, DOI: 10.1093/jb/mvz056, PubMed: 31504617, Elsevier: Scopus) Tatsuya Fukuta, Shintaroh Yoshimi, Tamotsu Tanaka and Kentaro Kogure :
Leukocyte-mimetic liposomes possessing leukocyte membrane proteins pass through inflamed endothelial cell layer by regulating intercellular junctions,
International Journal of Pharmaceutics, Vol.563, 314-323, 2019.

(要約): Nanoparticles such as liposomes have been applied for the treatment of various diseases such as cancer and inflammatory diseases by utilizing the enhanced permeability and retention effect. However, their entry into inflammation sites is still limited since passive delivery of nanoparticles is often hampered by the presence of endothelial barriers. As leukocytes can pass through the inflamed endothelium via utilizing membrane protein functions, we hypothesized that incorporating leukocyte membrane proteins onto liposomal membranes may impart leukocyte-mimicking functions to liposomes, allowing for their adherence to and active passage through the inflamed endothelium. Herein, we developed leukocyte-mimetic liposomes (LM-Lipo) by leukocyte membrane protein transfer and evaluated their function in vitro. Transfer of membrane proteins from human leukemia cells onto liposomal membranes allowed for significant association of the liposomes with inflamed human endothelial cells, and subsequent passage through inflamed endothelial cell layer. The confocal images showed that LM-Lipo significantly induced vascular endothelial-cadherin displacement. These results indicate that LM-Lipo adhered to and regulated intercellular junctions of inflamed endothelial cell layer, resulting in passage through the layer, by mimicking the function of leukocytes. Furthermore, it is suggested that liposomes possessing leukocyte-like functions could be useful for drug delivery to inflammation sites by overcoming endothelial barriers.
(キーワード): Biomimetics / HL-60 Cells / Human Umbilical Vein Endothelial Cells / Humans / Inflammation / Intercellular Junctions / Leukocytes / Liposomes / Membrane Proteins
(徳島大学機関リポジトリ): ● Metadata: 114730
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijpharm.2019.04.027
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30978483; ● Search Scopus @ Elsevier (PMID): 30978483; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijpharm.2019.04.027

(徳島大学機関リポジトリ: 114730, DOI: 10.1016/j.ijpharm.2019.04.027, PubMed: 30978483) Tatsuharu Shimokawa, Mai Yoshida, Tatsuya Fukuta, Tamotsu Tanaka, Toshio Inagi and Kentaro Kogure :
Efficacy of high-affinity liposomal astaxanthin on up-regulation of age-related markers induced by oxidative stress in human corneal epithelial cells,
Journal of Clinical Biochemistry and Nutrition, Vol.64, No.1, 27-35, 2019.

(要約): Decreases in tear volume, unstable tear films and excessive tear evaporation are known to cause desiccation and hyperosmolar stress. These, in turn, induce oxidative stress that is thought to cause dry eye, which is also considered to be age-related disease. We hypothesized that oxidative stress induces up-regulation of age-related markers, and that the antioxidant astaxanthin prepared as a liposomal formulation may be a candidate for the treatment of dry eye. Herein, we examined age-related markers in an dry eye model, and evaluated the efficacy of high-affinity liposomes containing astaxanthin. The dry eye model showed desiccation time-dependent increases in reactive oxygen species. We confirmed the up-regulation of p53, p21 and p16 as a function of desiccation time. Pretreatment with both neutral and slightly-positively-charged astaxanthin liposomal formulations showed significant suppression of up-regulation of all markers, with the positively-charged liposomes exhibiting the greatest efficacy. Furthermore, positively-charged liposomes labeled with fluorescent dyes demonstrated much higher affinity to normal human corneal epithelial cells (HCECs) than neutral liposomes. Taken together, we confirmed the up-regulation of age-related markers, especially p16, in an dry eye model, and demonstrated the potential of high-affinity liposomal astaxanthin for the treatment of dry eye.
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.18-27
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30705509; ● Summary page in Scopus @ Elsevier: 2-s2.0-85059761043

(DOI: 10.3164/jcbn.18-27, PubMed: 30705509, Elsevier: Scopus) Manami Inoue, Yoko Okamoto, Yuta Atsumi, Masatoshi Shiojiri, Mayumi Hidaka, Tamotsu Tanaka, Toshihiko Tsutsumi, Naoki Shirasaka and Akira Tokumura :
Addition of high load of lysophosphatidic acid to standard and high-fat chows causes no significant changes of its circulating and peripheral tissue levels but affects body weight and visceral fat mass of mice.,
BioFactors, Vol.44, No.6, 548-557, 2018.

(要約): Oral administration of lysophosphatidic acid (LPA), a critical intercellular lipid mediator, exerts wound healing and antiulcer effects on gastrointestinal system. To evaluate effects of food-derived LPA on body homeostasis, we measured LPA levels by liquid chromatography-tandem mass spectrometry in chows, feces, plasma, liver, and visceral fat of mice fed a normal or high-fat chow supplemented with or without LPA-rich soybean phospholipids for 30 days. Reductions in daily body weight gains and visceral fat mass were mainly related to lower chow intake by mice fed the LPA-rich high-fat chow, whereas reduced body weight gains and fat mass were mainly related to decreased intestinal triacylglycerol absorption in mice fed LPA-rich chow. Our results showed no significant increase in plasma, liver, or adipose LPA levels, even if a quite high LPA concentration (2.0%) in chows was ingested daily, suggesting limited effects of food-derived LPA on the lumen side of the digestive tract. © 2018 BioFactors, 44(6):548-557, 2018.
(キーワード): Animals / Body Weight / Chromatography, Liquid / Diet / Dietary Supplements / Feces / ホメオスタシス (homeostasis) / Intestinal Absorption / Intra-Abdominal Fat / Liver / Lysophospholipids / Male / Mice / Mice, Inbred C57BL / Tandem Mass Spectrometry / Triglycerides
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/biof.1451
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30368958; ● Summary page in Scopus @ Elsevier: 2-s2.0-85055676743

(DOI: 10.1002/biof.1451, PubMed: 30368958, Elsevier: Scopus) Misuzu Ishikawa, Shota Hirai, Tatsusada Yoshida, Natsumi Shibuya, Susumu Hama, Yu Takahashi, Tatsuya Fukuta, Tamotsu Tanaka, Shinzo Hosoi and Kentaro Kogure :
Carotenoid Stereochemistry Affects Antioxidative Activity of Liposomes Co-encapsulating Astaxanthin and Tocotrienol,
Chemical & Pharmaceutical Bulletin, Vol.66, No.7, 714-720, 2018.

(要約): We previously found that antioxidative activity of liposomes co-encapsulating astaxanthin (Asx) and tocotrienols (T3s) was higher than the calculated additive activity, which results from intermolecular interactions between both antioxidants (J. Clin. Biochem. Nutr., 59, 2016, Kamezaki et al.). Herein, we conducted experiments to optimize Asx/α-T3 ratio for high antioxidative activity, and tried to elucidate details of intermolecular interaction of Asx with α-T3. Higher activity than calculated additive value was clearly observed at an Asx/α-T3 ratio of 2 : 1, despite two α-T3 would potentially interact with two terminal rings of one Asx. The synthetic Asx used in this study was a mixture of three stereoisomers, 3R,3'R-form (Asx-R), 3S,3'S-form (Asx-S) and 3R,3'S-meso form (Asx-meso). The calculated binding energy of the Asx-S/α-T3 complex was higher than those of Asx-R/α-T3 and Asx-meso/α-T3, suggesting that Asx-S and α-T3 is the most preferable combination for the intermolecular interaction. The optimal Asx-S/α-T3 ratio for antioxidation was shown to be 1 : 2. These results suggest that the Asx stereochemistry affects the intermolecular interaction of Asx/α-T3. Moreover, the absorption spectrum changes of Asx-S upon co-encapsulation with α-T3 in liposomes indicate that the electronic state of Asx-S is affected by intermolecular interactions with α-T3. Further, intermolecular interactions with α-T3 affected the electronic charges on the C9, C10 and C15 atoms in the polyene moiety of Asx-S. In conclusion, the intermolecular interaction of Asx/T3 depends on the Asx stereochemistry, and caused a change in the electronic state of the Asx polyene moiety by the presence of double bond in the T3 triene moiety.
(キーワード): Antioxidants / カロテノイド (carotenoids) / リポソーム (liposomes) / 分子構造 (molecular structure) / Stereoisomerism / Tocotrienols / Xanthophylls
(徳島大学機関リポジトリ): ● Metadata: 113562
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/cpb.c18-00035
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29962454; ● Summary page in Scopus @ Elsevier: 2-s2.0-85049232130

(徳島大学機関リポジトリ: 113562, DOI: 10.1248/cpb.c18-00035, PubMed: 29962454, Elsevier: Scopus) Sheuli Afroz, Ayano Yagi, Kouki Fujikawa, M. Motiur Rahman, Katsuya Morito, Tatsuya Fukuta, Shiro Watanabe, Kazunori Toida, Emi Kiyokage, Taro Shimizu, Tatsuhiro Ishida, Kentaro Kogure, Akira Tokumura and Tamotsu Tanaka :
Lysophosphatidic acid in medicinal herbs enhances prostaglandin E2 and protects against indomethacin-induced gastric cell damage in vivo and in vitro,
Prostaglandins & Other Lipid Mediators, Vol.135, 36-44, 2018.

(要約): Lysophosphatidic acid (LPA) is a bioactive phospholipid that induces diverse biological responses. Recently, we found that LPA ameliorates NSAIDs-induced gastric ulcer in mice. Here, we quantified LPA in 21 medicinal herbs used for treatment of gastrointestinal (GI) disorders. We found that half of them contained LPA at relatively high levels (40-240 μg/g) compared to soybean seed powder (4.6 μg/g), which we previously identified as an LPA-rich food. The LPA in peony (Paeonia lactiflora) root powder is highly concentrated in the lipid fraction that ameliorates indomethacin-induced gastric ulcer in mice. Synthetic 18:1 LPA, peony root LPA and peony root lipid enhanced prostaglandin E production in a gastric cancer cell line, MKN74 cells that express LPA abundantly. These materials also prevented indomethacin-induced cell death and stimulated the proliferation of MKN74 cells. We found that LPA was present in stomach fluids at 2.4 μM, which is an effective LPA concentration for inducing a cellular response in vitro. These results indicated that LPA is one of the active components of medicinal herbs for the treatment of GI disorder and that orally administered LPA-rich herbs may augment the protective actions of endogenous LPA on gastric mucosa.
(徳島大学機関リポジトリ): ● Metadata: 112025
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.prostaglandins.2018.01.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29462674; ● Summary page in Scopus @ Elsevier: 2-s2.0-85042401633

(徳島大学機関リポジトリ: 112025, DOI: 10.1016/j.prostaglandins.2018.01.003, PubMed: 29462674, Elsevier: Scopus) Manami Inoue, Kazuhito Tsuboi, Yoko Okamoto, Mayumi Hidaka, Toru Uyama, Toshihiko Tsutsumi, Tamotsu Tanaka, Natsuo Ueda and Akira Tokumura :
Peripheral tissue levels and molecular species compositions of N-acyl-phosphatidylethanolamine and its metabolites in mice lacking N-acyl-phosphatidylethanolamine-specific phospholipase D.,
The Journal of Biochemistry, Vol.162, No.6, 449-458, 2017.

(要約): N-acylethanolamines (NAEs), a class of lipid mediators, are produced from N-acyl-phosphatidylethanolamine (NAPE) by several pathways, including the direct release by NAPE-specific phospholipase D (NAPE-PLD) or the multistep pathway via sn-glycero-3-phospho-N-acylethanolamine (Gp-NAE). Using liquid chromatography-tandem mass spectrometry, we compared peripheral tissue levels of NAPE, Gp-NAE and NAE in NAPE-PLD-deficient (NAPE-PLD-/-) and wild type (WT) mice. NAPE-PLD was suggested to play a major role in the NAPE degradation in heart, kidney, and liver, but not in jejunum, because the NAPE levels except jejunum were significantly higher in NAPE-PLD-/- mice than in WT mice. The deletion of NAPE-PLD failed to alter the NAE levels of these tissues, suggesting its limited role in the NAE production. The enzyme assays with tissue homogenates confirmed the presence of NAPE-PLD-independent pathways in these peripheral tissues. Gp-NAE species having an acyl moiety with 22 carbons and 6 double bonds was enriched in these peripheral tissues. As for sn-2 acyl species of NAPE, 18:2-acyl-containing NAPE species were predominant over 18:1-containing species in heart, liver, and jejunum. Our results show that both molecular species composition of NAPE, NAE and Gp-NAE and their dependencies on Napepld are different among the peripheral tissues, suggesting that each tissue has distinct metabolic pathways and these NAE-containing lipids play tissue-specific roles.
(キーワード): Animals / 脳 (brain) / Ethanolamines / 心臓 (heart) / Jejunum / Kidney / Lipids / Liver / Mice / Mice, Inbred C57BL / ノックアウトマウス (knockout mice) / 分子構造 (molecular structure) / Phosphatidylethanolamines / Phospholipase D
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvx054
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28992041; ● Summary page in Scopus @ Elsevier: 2-s2.0-85029655027

(DOI: 10.1093/jb/mvx054, PubMed: 28992041, Elsevier: Scopus) Kida Takashi, Itoh Aoi, Kimura Akari, Matsuoka Hisatsugu, Imai Hiroyuki, Kentaro Kogure, Akira Tokumura and Tamotsu Tanaka :
Distribution of glycosylinositol phosphoceramide-specific phospholipase D activity in plants,
The Journal of Biochemistry, Vol.161, No.2, 187-195, 2017.

(要約): Previously, we detected an unknown sphingophospholipid in cabbage leaves and identified it as phytoceramide-1-phosphate (PC1P). We also found an enzyme activity that produces PC1P by glycosylinositol phosphoceramide (GIPC)-specific hydrolysis in cabbage leaves. To characterize the GIPC-specific phospholipase D (GIPC-PLD) activity, we investigated distributions of GIPC-PLD activity in 25 tissues of 10 plants. In most plants, the GIPC-PLD activity was the highest in roots. Young leaves of cabbage and Welsh onion had higher activities than corresponding aged outer leaves. The GIPC-PLD activities in leaves, stems and roots of mung bean were higher in the sprouting stage than in more mature stages. We also examined the distribution of substrate GIPC and product PC1P and found that GIPC was ubiquitously distributed at 50-280 nmol/g (wet wt) in tissues of plants, whereas PC1P was detectable (3-60 nmol/g wet wt.) only in tissues showing considerable GIPC-PLD activity. These results suggest a possibility that GIPC-PLD activity is involved in plant growth.
(キーワード): Brassica / Ceramides / Daucus carota / Glycosphingolipids / Molecular Structure / Phospholipase D / Plant Leaves / Raphanus / Spinacia oleracea
(徳島大学機関リポジトリ): ● Metadata: 117827
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvw060
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28175321; ● CiNii @ 国立情報学研究所 (CRID): 1522262180608136704; ● Summary page in Scopus @ Elsevier: 2-s2.0-85015919191

(徳島大学機関リポジトリ: 117827, DOI: 10.1093/jb/mvw060, PubMed: 28175321, CiNii: 1522262180608136704, Elsevier: Scopus) S Afroz, Teru Ikoma, Ayano Yagi, Kentaro Kogure, Akira Tokumura and Tamotsu Tanaka :
Concentrated phosphatidic acid in cereal brans as potential protective agents against indomethacin-induced stomach ulcer.,
Journal of Agricultural and Food Chemistry, Vol.64, No.37, 6950-6957, 2016.

(要約): One of complications associated with long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) is peptic ulcer. Recently, we found that orally administered phosphatidic acid (PA) ameliorated aspirin-induced stomach lesions in mice. In this study, we identified PA-rich food sources and examined the effects of the food materials on indomethacin-induced stomach ulcer. Among examined, buckwheat (Fagopyrum esculentum) bran contained the highest level of PA (188 mg/100 g). PA was the richest phospholipid (25%) in the lipid fraction of the buckwheat bran. Administration of the lipid extracts of buckwheat bran significantly ameliorated indomethacin-induced stomach lesions in mice. In contrast, wheat (Triticum durum) bran lipids (PA, 4%) and soybean (Glycine max) lipids (PA, 3%) were not associated with ameliorative effects. These results indicated that PA-rich lipids can be used as an effective supplement for prevention of NSAID-induced stomach ulcer.
(キーワード): Animals / Edible Grain / Fagopyrum / Gastric Mucosa / Humans / Indomethacin / Male / Mice / Mice, Inbred ICR / Phosphatidic Acids / Plant Extracts / Protective Agents / Seeds / Soybeans / Stomach Ulcer / Triticum
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/acs.jafc.6b02884
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27561232; ● Search Scopus @ Elsevier (PMID): 27561232; ● Search Scopus @ Elsevier (DOI): 10.1021/acs.jafc.6b02884

(DOI: 10.1021/acs.jafc.6b02884, PubMed: 27561232) Iffat Sonia Ara Rahman, Kazuhito Tsuboi, Zahir Hussain, Ryouhei Yamashita, Yoko Okamoto, Toru Uyama, Naoshi Yamazaki, Tamotsu Tanaka, Akira Tokumura and Natsuo Ueda :
Calcium-dependent generation of N-acylethanolamines and lysophosphatidic acids by glycerophosphodiesterase GDE7.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1861, No.12 pt A, 1881-1892, 2016.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2016.09.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27637550; ● Summary page in Scopus @ Elsevier: 2-s2.0-84988489470

(DOI: 10.1016/j.bbalip.2016.09.008, PubMed: 27637550, Elsevier: Scopus) Mahadi Hasan, Noriko Saito-Tarashima, Koki Fujikawa, Takashi Ohgita, Susumu Hama, Tamotsu Tanaka, Hiroyuki Saito, Noriaki Minakawa and Kentaro Kogure :
The novel functional nucleic acid iRed effectively regulates target genes following cytoplasmic delivery by faint electric treatment,
Science and Technology of Advanced Materials, Vol.17, No.17, 554-562, 2016.

(要約): An intelligent shRNA expression device (iRed) contains the minimum essential components needed for shRNA production in cells, and could be a novel tool to regulate target genes. However, general delivery carriers consisting of cationic polymers/lipids could impede function of a newly generated shRNA via electrostatic interaction in the cytoplasm. Recently, we found that faint electric treatment (fET) of cells enhanced delivery of siRNA and functional nucleic acids into the cytoplasm in the absence of delivery carriers. Here, we examined fET of cells stably expressing luciferase in the presence of iRed encoding anti-luciferase shRNA. Transfection of lipofectamine 2000 (LFN)/iRed lipoplexes showed an RNAi effect, but fET-mediated iRed transfection did not, likely because of the endosomal localization of iRed after delivery. However, fET in the presence of lysosomotropic agent chloroquine significantly improved the RNAi effect of iRed/fET to levels that were higher than those for the LFN/iRed lipoplexes. Furthermore, the amount of lipid droplets in adipocytes significantly decreased following fET with iRed against resistin in the presence of chloroquine. Thus, iRed could be a useful tool to regulate target genes following fET-mediated cytoplasmic delivery with endosomal escape devices.
(徳島大学機関リポジトリ): ● Metadata: 115720
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/14686996.2016.1221726
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27877903; ● Summary page in Scopus @ Elsevier: 2-s2.0-85019070861

(徳島大学機関リポジトリ: 115720, DOI: 10.1080/14686996.2016.1221726, PubMed: 27877903, Elsevier: Scopus) Junpei Yamamoto, Midori Omura, Koichiro Tuchiya, Mayumi Hidaka, Akira Kuwahara, Minoru Irahara, Tamotsu Tanaka and Akira Tokumura :
Preferable existence of polyunsaturated lysophosphatidic acids in human follicular fluid from patients programmed with in vitro fertilization.,
Prostaglandins & Other Lipid Mediators, Vol.126, 16-23, 2016.

(要約): Lysophosphatidic acid (LPA) exerts diverse physiological effects on various types of animal cells, including reproductive cells, through its binding to six LPA receptors. We previously found that LPA promoted maturation of the nucleus and cytoplasm of mouse and hamster oocytes surrounded by cumulus cells in vitro. Using gas-liquid chromatography, we previously reported detection of several species of LPA by analyzing the fatty acid methyl esters derived from thin layer chromatography-purified LPA in lipid extract from incubated follicular fluids programmed with in vitro fertilization. In this study using liquid chromatography- tandem mass spectrometry, we directly detected high levels of linoleoyl, arachidonoyl, and docosahexaenoyl LPAs in human follicular fluid. This unique molecular species composition of LPA was suggested to be due to a balance between the low LPA-degrading activity and high LPA-producing activity of autotaxin in human follicular fluid. Our results suggest that polyunsaturated LPAs produced by autotaxin in human follicular fluid exert unknown physiological effects on cumulus cells.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.prostaglandins.2016.07.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27421691; ● Summary page in Scopus @ Elsevier: 2-s2.0-84978805217

(DOI: 10.1016/j.prostaglandins.2016.07.008, PubMed: 27421691, Elsevier: Scopus) Toshihiko Tsutsumi, Yoko Okamoto, Syougo Yamakawa, Cheng Bingjun, Akira Ishihara, Tamotsu Tanaka and Akira Tokumura :
Reduced rat plasma lysophosphatidylglycerol or lysophosphatidic acid level as a biomarker of aristolochic acid-induced renal and adipose dysfunctions.,
Life Sciences, Vol.157, 208-216, 2016.

(要約): Food products and diet pills containing aristolochic acid (AA) are responsible for a rapid progression of nephropathy associated with reduced body weight in human beings. In this study, we investigated the relationship of dietary NaCl and lysophospholipid (LPL) plasma levels to body weight gain in AA-treated rats. Male rats receiving a salt-deficient chow, normal salt chow or high salt chow were injected intraperitoneally daily with AA for 15days. Body weight, visceral fat mass, food intake, levels of LPL in plasma and its synthesized enzyme were investigated. Body weight gain, visceral fat mass and daily food intake were smaller in AA-treated rats than those of control rats, regardless of dietary salt concentration. AA treatment decreased plasma levels of major lysophosphatidic acid (LPA) molecular species in rats fed the normal or high-salt chow but not the salt-deficient chow, whereas both the plasma lysophospholipase D activity and kidney mRNA level of autotaxin of AA-treated rats fed chow with defined salt concentrations were lower than those of control rats. Plasma levels of major molecular species of lysophosphatidylglycerol (LPG) in AA-treated rat groups fed chow with defined salt concentrations were lower than those of control rats. Plasma levels of LPG and LPA seem to be relevant to the reduced body weight gain and fat mass due to AA treatment.
(キーワード): Adipose Tissue / Animals / Aristolochic Acids / Biomarkers / Body Weight / Feeding Behavior / Kidney / Lysophospholipids / Male / Organ Size / Phosphoric Diester Hydrolases / RNA, Messenger / Rats / Rats, Wistar / Sodium Chloride, Dietary
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2016.06.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27267499; ● Summary page in Scopus @ Elsevier: 2-s2.0-84975841169

(DOI: 10.1016/j.lfs.2016.06.003, PubMed: 27267499, Elsevier: Scopus) 喜田孝史, 木村朱里, 伊藤葵, 山下量平, 小暮健太朗, 德村彰, 田中保 :
食品に含まれるグリコシルイノシトールホスホセラミドおよびフィトセラミド-1-リン酸,
脂質栄養学, Vol.25, 75-85, 2016年.

(出版サイトへのリンク): ● Publication site (DOI): 10.4010/jln.25.75
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205191889280; ● Search Scopus @ Elsevier (DOI): 10.4010/jln.25.75

(DOI: 10.4010/jln.25.75, CiNii: 1390001205191889280) Ryouhei Yamashita, Yumika Tabata, Erina Iga, Michiyasu Nakao, Shigeki Sano, Kentaro Kogure, Akira Tokumura and Tamotsu Tanaka :
Analysis of molecular species profiles of ceramide-1-phosphate and sphingomyelin using MALDI-TOF mass spectrometry,
Lipids, Vol.51, No.2, 263-270, 2016.

(要約): Ceramide-1-phosphate (C1P) is a potential signaling molecule that modulates various cellular functions in animals. It has been known that C1P with different N-acyl lengths induce biological responses differently. However, molecular species profiles of the C1P in animal tissues have not been extensively examined yet. Here, we developed a method for determination of the molecular species of a C1P using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with Phos-tag, a phosphate capture molecule. The amounts of total C1P in skin, brain, liver, kidney and small intestine of mice were determined to be 344, 151, 198, 96 and 90 pmol/g wet weight, respectively. We found a C1P species having an α-hydroxypalmitoyl residue (h-C1P, 44 pmol/g wet weight) in mouse skin. The h-C1P was detected only in the skin, and not other tissues of mice. The same analysis was applied to sphingomyelin after conversion of sphingomyelin to C1P by Streptomyces chromofuscus phospholipase D. We found that molecular species profiles of sphingomyelin in skin, kidney and small intestine of mice were similar to those of C1P in corresponding tissues. In contrast, molecular species profiles of sphingomyelin in liver and brain were quite different from those of C1P in these tissues, indicating selective synthesis or degradation of C1P in these tissues. The method described here will be useful for detection of changes in molecular species profiles of C1P and sphingomyelin.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11745-015-4082-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26694604; ● Summary page in Scopus @ Elsevier: 2-s2.0-84957439211

(DOI: 10.1007/s11745-015-4082-0, PubMed: 26694604, Elsevier: Scopus) Toshihiko Tsutsumi, Syougo Yamakawa, Akira Ishihara, Aimi Yamamoto, Tamotsu Tanaka and Akira Tokumura :
Reduced kidney levels of lysophosphatidic acids in rats after chronic administration of aristolochic acid: Its possible protective role in renal fibrosis,
Toxicology Reports, Vol.2, 121-129, 2015.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.toxrep.2015.02.012
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84924599379

(DOI: 10.1016/j.toxrep.2015.02.012, Elsevier: Scopus) Yoshibumi Shimizu, Yoshiyuki Morikawa, Shinichi Okudaira, Shigenobu Kimoto, Tamotsu Tanaka, Junken Aoki and Akira Tokumura :
Potentials of the circulating pruritogenic mediator lysophosphatidic acid in development of allergic skin inflammation in mice: role of blood cell-associated lysophospholipase D activity of autotaxin.,
The American Journal of Pathology, Vol.184, No.5, 1593-1603, 2014.

(要約): Itching and infiltration of immune cells are important hallmarks of atopic dermatitis (AD). Although various studies have focused on peripheral mediator-mediated mechanisms, systemic mediator-mediated mechanisms are also important in the pathogenesis and development of AD. Herein, we found that intradermal injection of lysophosphatidic acid (LPA), a bioactive phospholipid, induces scratching responses by Institute of Cancer Research mice through LPA1 receptor- and opioid μ receptor-mediating mechanisms, indicating its potential as a pruritogen. The circulating level of LPA in Naruto Research Institute Otsuka Atrichia mice, a systemic AD model, with severe scratching was found to be higher than that of control BALB/c mice, probably because of the increased lysophospholipase D activity of autotaxin (ATX) in the blood (mainly membrane associated) rather than in plasma (soluble). Heparan sulfate proteoglycan was shown to be involved in the association of ATX with blood cells. The sequestration of ATX protein on the blood cells by heparan sulfate proteoglycan may accelerate the transport of LPA to the local apical surface of vascular endothelium with LPA receptors, promoting the hyperpermeability of venules and the pathological uptake of immune cells, aggravating lesion progression and itching in Naruto Research Institute Otsuka Atrichia mice.
(キーワード): Animals / Blood Cells / Cell Membrane / Chromatography, Liquid / Hypersensitivity / 炎症 (inflammation) / Lysophospholipids / Male / 質量分析法 (mass spectrometry) / Mice / Mice, Inbred BALB C / Phosphoric Diester Hydrolases / Protein Binding / Pruritus / Skin / 溶解度 (solubility) / Sphingosine
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ajpath.2014.01.029
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24641902; ● Summary page in Scopus @ Elsevier: 2-s2.0-84899540615

(DOI: 10.1016/j.ajpath.2014.01.029, PubMed: 24641902, Elsevier: Scopus) Tamotsu Tanaka, Sachika Uozumi, Katsuya Morito, Takashi Osumi and Akira Tokumura :
Metabolic conversion of C20 polymethylene-interrupted polyunsaturated fatty acids to essential fatty acids.,
Lipids, Vol.49, No.5, 423-429, 2014.

(要約): Polymethylene-interrupted (PMI)-polyunsaturated fatty acids (PUFA) are fatty acids present largely in gymnosperm. Sciadonic acid (SciA, 20:3 Δ-5,11,14) and juniperonic acid (JA, 20:4 Δ-5,11,14,17) are typical C20 PMI-PUFA with an isolated double bond at Δ5. Previously, we found that SciA and JA are converted to linoleic acid (LNA) and α-linolenic acid (ΑLA), respectively. The conversion process includes chain-shortening step by peroxisomal β-oxidation for elimination a double bond at Δ5, and subsequent chain-elongation step in microsomes. In this study, we examined the substrate specificity of this metabolism in rodent and human cells. Supplementation of SciA, eicosadienoic acid (EDA, 20:2 Δ-11,14) or JA to CHO-K1 cells (wild type) induced an accumulation of LNA, LNA or ALA, respectively, in cellular lipids. These changes were not observed in the peroxisomes-deficient CHO cells, indicating involvement of peroxisomes in the metabolism. Two types of human cells (MKN74 and HepG2) also converted the C20 PMI-PUFA and EDA to the respective essential fatty acids. In contrast, no chain-shortened metabolite of pinolenic acid (18:3 Δ-5,9,12) was detected in any cell lines tested. From these results, C20 PMI-PUFA and EDA, but not C18 PMI-PUFA, are suggested as being effectively converted to essential fatty acids by the fatty acid remodeling system in rodent and human cells.
(キーワード): Animals / Arachidonic Acids / CHO Cells / Cells, Cultured / Cricetulus / Fatty Acids, Essential / Fatty Acids, Unsaturated / Hep G2 Cells / Humans
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11745-014-3896-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24659112; ● Summary page in Scopus @ Elsevier: 2-s2.0-84900792021

(DOI: 10.1007/s11745-014-3896-5, PubMed: 24659112, Elsevier: Scopus) Tamotsu Tanaka, Mayumi Ohmoto, Katsuya Morito, H. Kondo, Mai Urikura, Kiyoshi Satouchi and Akira Tokumura :
Type 2 lysophosphatidic acid receptor in gastric surface mucous cells: Possible implication of prostaglandin E2 production,
BioFactors, Vol.40, No.3, 355-361, 2014.

(要約): Lysophosphatidic acid (LPA) is a lipid mediator that induces various cell responses via its specific receptors. Recently, we found that orally administered LPA and phosphatidic acid (PA) ameliorate stress- or aspirin-induced stomach injury. However, the mechanisms underlying these effects have not been elucidated yet. In this study, we examined effect of LPA on prostaglandin (PG) E2 production in MKN74 cells, a gastric cell-line expressing type 2 LPA receptor (LPA2). When the cells were treated with LPA, the level of mRNA of COX-2 but not COX-1 was upregulated. The LPA effect was abolished when the cells were pretreated with pertussis toxin (PTX), suggesting the involvement of receptor(s) coupled with Gi. Pretreatment of MKN74 cells with LPA enhanced the PGE2 production triggered by calcium ionophore A23187. Again, PTX abolished the LPA effect. Fluorescent immunohistochemistry using an antibody against LPA2 showed that surface mucous cells (pit cells) in gastric mucosa of mice express LPA2 on the apical side of the plasma membrane. These results suggest that LPA in the diet or its digestion may contribute to the epithelial integrity of stomach mucosa by enhancement of PGE2 production via activation of LPA2. © 2013 BioFactors, 2013.
(キーワード): Animals / Cell Line / Cell Polarity / Cyclooxygenase 1 / Cyclooxygenase 2 / Dinoprostone / Epithelial Cells / Gastric Mucosa / Gene Expression / Humans / Lysophospholipids / Male / Mice, Inbred ICR / Receptors, Lysophosphatidic Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/biof.1147
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24375908; ● Summary page in Scopus @ Elsevier: 2-s2.0-84902549036

(DOI: 10.1002/biof.1147, PubMed: 24375908, Elsevier: Scopus) Yoshibumi Shimizu, Kazutoshi Murao, Tamotsu Tanaka, Yoshiaki Kubo and Akira Tokumura :
Increased lysophospholipase D activity of autotaxin in sera of patients with atopic dermatitis.,
Journal of Dermatological Science, Vol.74, No.2, 162-165, 2014.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jdermsci.2014.01.010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24582488; ● Summary page in Scopus @ Elsevier: 2-s2.0-84897115979

(DOI: 10.1016/j.jdermsci.2014.01.010, PubMed: 24582488, Elsevier: Scopus) Tamotsu Tanaka, T. Kida, H. Imai, J. Morishige, R. Yamashita, H. Matsuoka, S. Uozumi, K. Satouchi, M. Nagano and Akira Tokumura :
Identification of a sphingolipid-specific phospholipase D activity associated with the generation of phytoceramide-1-phosphate in cabbage leaves,
The FEBS Journal, Vol.280, No.16, 3797-3809, 2013.

(要約): The structure and biosynthetic route for an unidentified lipid (lipid X) detected by TLC of cabbage (Brassica oleracea) lipids was determined. Lipid X is a phospholipid that is resistant to mild alkali and detectable by MALDI-TOF MS as an adduct with Phos-tag, a phosphate-capture zinc complex. Various α-hydroxy fatty acids (16:0, 22:0, 24:0 and 24:1) were detected by GC-MS of fatty acid methyl esters prepared from lipid X. The deacyl derivative of lipid X was determined to be 4-hydroxysphingenine (dehydrophytosphingosine)-1-phosphate by MALDI-TOF MS with Phos-tag. From these results, lipid X was determined to be phytoceramide-1-phosphate (PC1P) with an α-hydroxy fatty acid. When cabbage homogenates were incubated, PC1P was formed, with a concomitant decrease in the amount of glycosylinositol phosphoceramide (GIPC). The formation of PC1P from GIPC was confirmed by treatment of purified cabbage GIPC with a membrane fraction of cabbage homogenates. Using a partially purified enzyme fraction, we found that the enzyme hydrolyzes GIPC specifically, but not glycerophospholipids and sphingomyelin. Arabidopsis thaliana also had this enzyme activity. From these results, we conclude that a previously uncharacterized phospholipase D activity that specifically hydrolyzes GIPC produces PC1P in brassicaceous plants.
(キーワード): Arabidopsis Proteins / Brassica / Ceramides / Chromatography, Thin Layer / Gas Chromatography-Mass Spectrometry / Glycosphingolipids / Glycosylation / Kinetics / Membrane Proteins / Phospholipase D / Plant Leaves / Plant Proteins / Spectrometry, Mass, Electrospray Ionization / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Sphingolipids / Subcellular Fractions / Substrate Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/febs.12374
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23738625; ● Search Scopus @ Elsevier (PMID): 23738625; ● Search Scopus @ Elsevier (DOI): 10.1111/febs.12374

(DOI: 10.1111/febs.12374, PubMed: 23738625) Tamotsu Tanaka, K. Morito, M. Kinoshita, M. Ohmoto, M. Urikura, K. Satouchi and Akira Tokumura :
Orally administered phosphatidic acids and lysophosphatidic acids ameliorate aspirin-induced stomach mucosal injury in mice,
Digestive Diseases and Sciences, Vol.58, No.4, 950-958, 2013.

(要約): Recent investigations revealed that lysophosphatidic acid (LPA), a phospholipid with a growth factor-like activity, plays an important role in the integrity of the gastrointestinal tract epithelium. This paper attempts to clarify the effect of orally administered phosphatidic acid (PA) and LPA on aspirin-induced gastric lesions in mice. Phospholipids, a free fatty acid, a diacylglycerol and a triglyceride at 1 mM (5.7 μmol/kg body weight) or 0.1 mM were orally administered to mice 0.5 h before oral administration of aspirin (1.7 mmol/kg). The total length of lesions formed on the stomach wall was measured as a lesion index. Formation of LPA from PA in the mouse stomach was examined by in vitro (in stomach lavage fluid), ex vivo (in an isolated stomach) and in vivo (in the stomach of a living mouse) examinations of phospholipase activity. Palmitic acid, dioleoyl-glycerol, olive oil and lysophosphatidylcholine did not affect the aspirin-induced lesions. In contrast, phosphatidylcholine (1 mM), LPA (1 mM) and PA (0.1, 1 mM) significantly reduced the lesion index. Evidence for formation of LPA from PA in the stomach by gastric phospholipase A2 was obtained by in vitro, ex vivo and in vivo experiments. An LPA-specific receptor, LPA2, was found to be localized on the gastric surface-lining cells of mice. Pretreatment with PA-rich diets may prevent nonsteroidal anti-inflammatory drug-induced stomach ulcers.
(キーワード): Administration, Oral / Animals / Anti-Inflammatory Agents, Non-Steroidal / Aspirin / Drug Evaluation, Preclinical / Lysophospholipids / Male / Mice / Mice, Inbred ICR / Phosphatidic Acids / Phospholipases A2 / Receptors, Lysophosphatidic Acid / Stomach / Stomach Ulcer
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10620-012-2475-y
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23161268; ● Search Scopus @ Elsevier (PMID): 23161268; ● Search Scopus @ Elsevier (DOI): 10.1007/s10620-012-2475-y

(DOI: 10.1007/s10620-012-2475-y, PubMed: 23161268) Mai Urikura, Jun-ichi Morishige, Tamotsu Tanaka and Kiyoshi Satouchi :
Phosphatidic acid production in the processing of cabbage leaves,
Journal of Agricultural and Food Chemistry, Vol.60, No.45, 11359-11365, 2012.

(要約): Lysophosphatidic acid (LPA) is a lipid mediator involved in various physiological responses, including wound healing. Evidence of the antiulcer activity of LPA has been reported, and soybean LPA at a concentration of 10 μM is effective in reducing stress-induced gastric ulcer. Because LPA can be formed from phosphatidic acid (PA) by digestive phospholipase A2, dietary PA can be considered a potential antiulcer phospholipid. In this study, PA production in cut processing of cabbage leaves was examined. The amounts of PA in sliced, minced, and homogenized cabbage leaves were 107 ± 5, 134 ± 19, and 286 ± 29 nmol PA/g (wet weight), respectively, all being significantly higher than the amount of PA found in intact leaves. Mixing mayonnaise with sliced cabbage dramatically increased the PA content (1586 ± 393 nmol/3 g), indicating phospholipase D activity leaked raw cabbage produced PA. These results indicate that fine cutting raw cabbage leaves and mixing them with foods rich in phospholipids resulted in an abundant production of PA.
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf303515z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23098184; ● Search Scopus @ Elsevier (PMID): 23098184; ● Search Scopus @ Elsevier (DOI): 10.1021/jf303515z

(DOI: 10.1021/jf303515z, PubMed: 23098184) Tamotsu Tanaka, Ayaka Kassai, Mayumi Ohmoto, Katsuya Morito, Yoshiki Kashiwada, Yoshihisa Takaishi, Mai Urikura, Jun-ichi Morishige, Kiyoshi Satouchi and Akira Tokumura :
Quantification of phosphatidic acid in foodstuffs using TLC-imaging technique,
Journal of Agricultural and Food Chemistry, Vol.60, No.16, 4156-4161, 2012.

(要約): Apical application of lysophosphatidic acid (LPA), a growth-factor-like phospholipid, was shown to prevent or restore gastrointestinal (GI) disorders, such as diarrhea and stomach ulcer, in experimental animals. Because LPA is formed from phosphatidic acid (PA) by the activity of digestive phospholipase A(2), PA is a potential component for dietary treatment of such GI disorders. Here, we quantified PA contained in 38 foodstuffs and 3 herbs by a thin-layer-chromatography-imaging technique. Vegetables belonging to Brassicaceae, such as cabbage leaves (700 nmol/g of wet weight) and Japanese radish leaves (570 nmol/g), contained higher amounts of PA than other foodstuffs. Amounts of PA in fruits, cereals, and starchy root vegetables were below 300 nmol/g. Animal foodstuffs contained low amounts of PA (<60 nmol/g). Interestingly, leaves of Mallotus japonicas, a Japanese edible herb used for treatment of stomach ulcer, had the highest PA (1410 nmol/g) among those examined. The data shown here will be useful for the development of dietary treatment for a damaged GI tract.
(キーワード): Chromatography, Thin Layer / Food Analysis / Phosphatidic Acids
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf300147y
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22475031; ● Summary page in Scopus @ Elsevier: 2-s2.0-84860316722

(DOI: 10.1021/jf300147y, PubMed: 22475031, Elsevier: Scopus) 田中保, 盛重純一, 瓜倉真衣, 德村彰, 里内清 :
Phos-tagを用いた活性リン脂質の質量分析,
生物物理化学, Vol.56, s37-42, 2012年.

(要約): Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are growth factor-like bioactive lipids having a phosphate monoester residue. Phos-tag can bind to them and be used both for purification and quantification. In a two-phase solvent system consisting of chloroform/methanol/water, addition of Phos-tag move LPA and S1P from a hydrophilic phase to a hydrophobic phase in the form of their Phos-tag complexes. Using this property, we developed a method for purification of LPA and S1P in biological materials by the phase separation technique. Advantages of use of Phos-tag for detection of LPA and S1P in MALDI-TOF MS are an increase in ionization efficiency and detection as a single-ion form. Homologues of LPA and S1P in natural samples can be quantified by MALDI-TOF MS by using internal standards.<br>
(キーワード): Phos-tag / MALDI-TOF MS / lysophosphatidic acid / sphingosine 1-phosphate / phospholipid
(出版サイトへのリンク): ● Publication site (DOI): 10.2198/sbk.56.s37
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679178944384; ● Search Scopus @ Elsevier (DOI): 10.2198/sbk.56.s37

(DOI: 10.2198/sbk.56.s37, CiNii: 1390282679178944384) Masaki Ino, Yoshibumi Shimizu, Tamotsu Tanaka and Akira Tokumura :
Alterations of plasma levels of lysophosphatidic acid in response to fasting of rats,
Biological & Pharmaceutical Bulletin, Vol.35, No.11, 2059-2063, 2012.

(要約): The aim of this study was to investigate the effect of fasting on in vivo plasma levels of lysophosphatidic acid (LPA), a physiologically important lysophospholipid mediator. We assayed to measure activities of an LPA-producing enzyme (lysophospholipase D) and LPA-degrading enzyme activities (lysophspholipase A, lipid phosphate phosphatase) in rat plasma or blood, by measuring choline, fatty acid and inorganic phosphate, respectively. Both LPA and its precursor lysophosphatidylcholine (LPC) were quantified by liquid chromatography-tandem mass spectrometry. Fasting of rats for 24 h decreased plasma concentrations of oleoyl-, linoleoyl-, arachidonoyl- and docosahexaenoyl-LPAs, but not palmitoyl- and stearoyl-LPAs, possibly due to decreased levels of corresponding LPCs in the plasma and elevated lipid phosphate phosphatase activity for LPAs in the blood. Our results indicate that the in vivo circulating levels of LPAs in rats are affected by fasting.
(キーワード): Animals / Fasting / Lysophospholipase / Lysophospholipids / Male / Phosphoric Diester Hydrolases / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.b12-00497
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23123475; ● Search Scopus @ Elsevier (PMID): 23123475; ● Search Scopus @ Elsevier (DOI): 10.1248/bpb.b12-00497

(DOI: 10.1248/bpb.b12-00497, PubMed: 23123475) MIka Adachi, Gou Horiuchi, Natsuki Ikematsu, Tamotsu Tanaka, Shigeki Sano, Kenji Fukuzawa and Akira Tokumura :
Intragastrically administrered lysophospatidic acid protect against gastric ulcer in rats under water-immersion restraint stress,
Digestive Diseases and Sciences, Vol.56, No.8, 2252-2261, 2011.

(要約): Lysophosphatidic acid exerts important physiological effects on many types of animal cells through its specific binding to several G protein-coupled receptors. In particular, its potent wound-healing effect has attracted much attention. To determine whether lysophosphatidic acids in a foodstuff and Chinese medicine are effective in protecting against gastric ulcer, we subjected rats to water-immersion restraint stress. Three direct administrations of a solution of lysophosphatidic acid with a C18 fatty acyl group to the rat stomach in a concentration range of 0.001-0.1 mM resulted in a significant reduction in the number of gastric ulcers induced during water-immersion restraint stress, and the potencies were as follows: linoleoyl species=α-linolenoyl species>oleoyl species. Intragastric administrations of a solution of highly purified lysophosphatidic acid from soybean lecithin significantly protected against the stress-induced gastric ulcers at lower concentrations than partially purified lysophosphatidic acid from soybean lecithin did. In addition, administration of a decocted solution of antyu-san, and lysophosphatidic acid-rich Chinese medicine, to the stomach was more effective in protecting against stress-induced ulcer than decoctations of antyu-san lacking the corydalis tuber component that is rich in lysophosphatidic acid. These results clearly show that lysophosphatidic acid is the effective component of soybean lecithin and antyu-san in protection against stress-induced gastric ulcer in the rat model, and suggest that daily intake of lysophosphatidic acid-rich foods or Chinese medicines may be beneficial for prevention of stress-induced gastric ulcer in human subjects.
(キーワード): Animals / Corydalis / Drugs, Chinese Herbal / Gastric Mucosa / Immersion / Lecithins / Lysophospholipids / Male / Rats / Rats, Wistar / Restraint, Physical / Soybeans / Stomach Ulcer / Stress, Psychological
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10620-011-1595-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21298479; ● Summary page in Scopus @ Elsevier: 2-s2.0-79960932906

(DOI: 10.1007/s10620-011-1595-0, PubMed: 21298479, Elsevier: Scopus) Kaori Ueda, Masanori Yoshihara, Michiyasu Nakao, Tamotsu Tanaka, Shigeki Sano, Kenji Fukuzawa and Akira Tokumura :
Evaluation of inhibitory actions of flavonols and related substances on lysophospholipase d activity of serum autotaxin by a convenient assay using a chromogenic substrate.,
Journal of Agricultural and Food Chemistry, Vol.58, No.10, 6053-6063, 2010.

(要約): Overproduction of lysophosphatidic acid (LPA) by lysophospholipase D/autotaxin (lysoPLD/ATX) is postulated to be involved in the promotion of cancer and atherosclerosis. A lysoPLD inhibitor may be utilized to ameliorate the LPA-related pathological conditions. In this study, a new assay was devised to quantify p-nitrophenol from hydrolysis of chromogenic substrate by serum lysoPLD without tedious lipid extraction procedures. Flavonols, phenolic acids, free fatty acids, and N-acyltyrosines inhibited lysoPLD activity in a micromolar range. They were classified into competitive, noncompetitive, or mixed type inhibitors. The results show that the low hydrophobicity of an inhibitor is a critical factor in its preference for the binding to a noncatalytic binding site over a catalytic binding site. Considering its reported bioavailability and the low dependency of its inhibitory activity on serum dilution, flavonol is likely to be a more effective lysoPLD inhibitor in human blood circulation in vivo than the other inhibitors including LPA.
(キーワード): Binding Sites / Binding, Competitive / Enzyme Inhibitors / Flavonols / Humans / Multienzyme Complexes / Phosphodiesterase I / Phosphoric Diester Hydrolases / Pyrophosphatases / Recombinant Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf904155a
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20415488; ● Search Scopus @ Elsevier (PMID): 20415488; ● Search Scopus @ Elsevier (DOI): 10.1021/jf904155a

(DOI: 10.1021/jf904155a, PubMed: 20415488) Jun-ichi Morishige, Mai Urikura, Haruko Takagi, Kaoru Hirano, Tohru Koike, Tamotsu Tanaka and Kiyoshi Satouchi :
A clean-up technology for the simultaneous determination of lysophosphatidic acid and sphingosine-1-phosphate by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a phosphate-capture molecule, Phos-tag.,
Rapid Communications in Mass Spectrometry: RCM, Vol.24, No.7, 1075-1084, 2010.

(要約): Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are growth factor-like lipids having a phosphate group. The concentrations of these mediator lipids in blood are considered to be potential biomarkers for early detection of cancer or vascular diseases. Here, we report a method for simultaneous determination of LPA and S1P using Phos-tag, a zinc complex that specifically binds to a phosphate-monoester group. Although both LPA and S1P are hydrophilic compounds, we found that they acquire hydrophobic properties when they form complexes with Phos-tag. Based on this finding, we developed a method for the enrichment of LPA and S1P from biological samples. The first partition in a two-phase solvent system consisting of chloroform/methanol/water (1:1:0.9, v/v/v) is conducted for the removal of lipids. LPA and S1P are specifically extracted as Phos-tag complexes at the second partition by adding Phos-tag. The Phos-tag complexes of LPA and S1P are detectable by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and quantifiable based on the relative intensities of ions using 17:0 LPA and C17 S1P as internal standards. The protocol was validated by analyses of these mediator lipids in calf serum, a rat brain and a lung. The clean-up protocol is rapid, requires neither thin-layer chromatography (TLC) nor liquid chromatography (LC), and is applicable to both blood and solid tissue samples. We believe that our protocol will be useful for a routine analysis of LPA and S1P in many clinical samples.
(キーワード): Animals / Brain Chemistry / Cattle / Chemical Fractionation / Chloroform / Hydrogen-Ion Concentration / Lung / Lysophospholipids / Pyridines / Rats / Sensitivity and Specificity / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Sphingosine
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rcm.4484
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20213695; ● Search Scopus @ Elsevier (PMID): 20213695; ● Search Scopus @ Elsevier (DOI): 10.1002/rcm.4484

(DOI: 10.1002/rcm.4484, PubMed: 20213695) Noriko Takuwa, Sei-Ichiro Ohkura, Shin-Ichiro Takashima, Keisuke Ohtani, Yasuo Okamoto, Tamotsu Tanaka, Kaoru Hirano, Soichiro Usui, Fei Wang, Wa Du, Kazuaki Yoshioka, Yoshiko Banno, Motoko Sasaki, Ikuyo Ichi, Miwa Okamura, Naotoshi Sugimoto, Kiyomi Mizugishi, Yasuni Nakanuma, Isao Ishii, Masayuki Takamura, Shuichi Kaneko, Shosuke Kojo, Kiyoshi Satouchi, Kunitoshi Mitumori, Jerold Chun and Yoh Takuwa :
S1P3-mediated cardiac fibrosis in sphingosine kinase 1 transgenic mice involves reactive oxygen species.,
Cardiovascular Research, Vol.85, No.3, 484-493, 2009.

(要約): AIMS: Sphingosine kinase 1 (SPHK1), its product sphingosine-1-phosphate (S1P), and S1P receptor subtypes have been suggested to play protective roles for cardiomyocytes in animal models of ischaemic preconditioning and cardiac ischaemia/reperfusion injury. To get more insight into roles for SPHK1 in vivo, we have generated SPHK1-transgenic (TG) mice and analysed the cardiac phenotype. METHODS AND RESULTS: SPHK1-TG mice overexpressed SPHK1 in diverse tissues, with a nearly 20-fold increase in enzymatic activity. The TG mice grew normally with normal blood chemistry, cell counts, heart rate, and blood pressure. Unexpectedly, TG mice with high but not low expression levels of SPHK1 developed progressive myocardial degeneration and fibrosis, with upregulation of embryonic genes, elevated RhoA and Rac1 activity, stimulation of Smad3 phosphorylation, and increased levels of oxidative stress markers. Treatment of juvenile TG mice with pitavastatin, an established inhibitor of the Rho family G proteins, or deletion of S1P3, a major myocardial S1P receptor subtype that couples to Rho GTPases and transactivates Smad signalling, both inhibited cardiac fibrosis with concomitant inhibition of SPHK1-dependent Smad-3 phosphorylation. In addition, the anti-oxidant N-2-mercaptopropyonylglycine, which reduces reactive oxygen species (ROS), also inhibited cardiac fibrosis. In in vivo ischaemia/reperfusion injury, the size of myocardial infarct was 30% decreased in SPHK1-TG mice compared with wild-type mice. CONCLUSION: These results suggest that chronic activation of SPHK1-S1P signalling results in both pathological cardiac remodelling through ROS mediated by S1P3 and favourable cardioprotective effects.
(キーワード): Animals / Fibrosis / Fluorescent Antibody Technique / Mice / Mice, Inbred C57BL / Mice, Transgenic / Myocardial Reperfusion Injury / Myocardium / Neuropeptides / Phosphotransferases (Alcohol Group Acceptor) / Quinolines / Reactive Oxygen Species / Receptors, Lysosphingolipid / rac GTP-Binding Proteins / rho GTP-Binding Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/cvr/cvp312
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19755413; ● Search Scopus @ Elsevier (PMID): 19755413; ● Search Scopus @ Elsevier (DOI): 10.1093/cvr/cvp312

(DOI: 10.1093/cvr/cvp312, PubMed: 19755413) Tamotsu Tanaka, Gou Horiuchi, Megumi Matsuoka, Kaoru Hirano, Akira Tokumura, Tohru Koike and Kiyoshi Satouchi :
Formation of lysophosphatidic acid, a wound-healing lipid, during digestion of cabbage leaves.,
Bioscience, Biotechnology, and Biochemistry, Vol.73, No.6, 1293-1300, 2009.

(要約): Lysophosphatidic acid (LPA) is a lipid mediator that plays a role in the process of wound healing in animal tissues, including the digestive tract. We determined LPA in several foodstuffs, and found that cabbage leaves were the richest source of LPA. We also found that, at 22 and 195 nmol/g (wet weight), LPA and phosphatidic acid (PA) were respectively formed during mastication of raw cabbage leaves and that the resulting PA was converted to LPA by pancreatic phospholipase A(2). The lipid extract obtained from ground cabbage leaves promoted the proliferation of Swiss 3T3 fibroblasts and the motility of HGC-27 cells, stomach-derived epithelial-like cells, at physiologically relevant concentrations. These activities of cabbage lipids were inhibited by Ki16425, an LPA-receptor antagonist. LPA formed during the digestion of cabbage leaves may be one of the components in the beneficial effect of ingested cabbage on a damaged digestive tract.
(キーワード): 3T3 Cells / Animals / Brassica / Cell Proliferation / Lysophospholipids / Mice / Plant Leaves / Wound Healing
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.80813
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19502739; ● CiNii @ 国立情報学研究所 (CRID): 1390282681457031808; ● Search Scopus @ Elsevier (PMID): 19502739; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.80813

(DOI: 10.1271/bbb.80813, PubMed: 19502739, CiNii: 1390282681457031808) Jun-ichi Morishige, Naoki Amano, Kaoru Hirano, Hiroaki Nishio, Tamotsu Tanaka and Kiyoshi Satouchi :
Inhibitory effect of juniperonic acid (Delta-5c,11c,14c,17c-20:4, omega-3) on bombesin-induced proliferation of Swiss 3T3 cells.,
Biological & Pharmaceutical Bulletin, Vol.31, No.9, 1786-1789, 2008.

(要約): Juniperonic acid (Delta-5c,11c,14c,17c-20:4, JA) is a polymethylene-interrupted (PMI) fatty acid that occurs in Biota orientalis. In this study, we found that JA has an antiproliferative activity. Swiss 3T3 cells were preloaded with fatty acids before stimulation with bombesin, a mitogenic neuropeptide, and proliferation of the cells was assessed by [(3)H]thymidine incorporation. Preloading of linoleic acid (Delta-9c,12c-18:2) significantly enhanced bombesin-induced proliferation. In contrast, preloading of eicosapentaenoic acid (Delta-5c,8c,11c,14c,17c-20:5, EPA) suppressed proliferation. Likewise, cells preloaded with JA showed a significantly curtailed response to bombesin. The antiproliferative potency of JA was equivalent to that of EPA. Sciadonic acid (Delta-5c,11c,14c-20:3), an omega-6 analogue of JA did not show antiproliferative activity, suggesting the importance of the omega-3 double bond rather than the PMI structure. The EPA-like activity of JA may be involved in the pharmaceutical activity of biota seeds, a psychoactive Chinese traditional medicine.
(キーワード): Animals / Arachidonic Acids / Bombesin / Cell Proliferation / Eicosapentaenoic Acid / Fatty Acids / Fatty Acids, Unsaturated / Lipid Metabolism / Mice / Phospholipids / Seeds / Swiss 3T3 Cells / Thuja
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.31.1786
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18758077; ● Search Scopus @ Elsevier (PMID): 18758077; ● Search Scopus @ Elsevier (DOI): 10.1248/bpb.31.1786

(DOI: 10.1248/bpb.31.1786, PubMed: 18758077) 吉積一真, 平野薫, 富裕孝, 田中保, 里内清 :
クローブ(syzygium aromaticum L.)熱水抽出物の高脂肪食摂餌マウス脂質代謝に及ぼす影響,
生薬学雑誌, Vol.61, No.2, 79-85, 2007年.

(要約): The effect of a hot water extract of clove (Syzygium aromaticum L.) on serum and hepatic lipids, and fecal triglyceride levels was investigated in mice. The addition of 1.0% (w/w) of the clove hot water extract to a high-fat diet containing 60% (w/w) lard for 28 days significantly increased fecal triglyceride levels, significantly reduced plasma insulin and hepatic total cholesterol levels, and tended to reduce plasma and hepatic triglyceride levels compared with mice given a high-fat diet without the clove hot water extract. Semiquantitative real time reverse transcription-polymerase chain reaction analysis demonstrated that addition of the clove hot water extract to a high-fat diet increased gene expression of medium-chain acyl-CoA dehydrogenase and reduced gene expression of fatty acid synthase in the liver. In addition, the clove hot water extract dose-dependently inhibited lipase activity in vitro. It is therefore suggested that the improvement action of lipid metabolism in mice fed a high-fat diet originated from the activation of lipid oxidation, the inactivation of fatty acid synthesis in the liver, and the inhibition of lipolysis in the small intestine.
(キーワード): clove / Syzygium aromaticum / liver lipid metabolism / lipase inhibitor / medium-chain acyl-CoA dehydrogenase / fatty acid synthase
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520009409858825984

(CiNii: 1520009409858825984) Tamotsu Tanaka, J. Morishige, D. Iwawaki, T. Fukuhara and K. Satouchi :
Metabolic pathway that produces essential fatty acids from polymethylene-interrupted polyunsaturated fatty acids in animal cells,
The FEBS Journal, Vol.274, No.11, 2728-2737, 2007.

(要約): Sciadonic acid (20:3 Delta-5,11,14) and juniperonic acid (20:4 Delta-5,11,14,17) are polyunsaturated fatty acids (PUFAs) that lack the Delta-8 double bond of arachidonic acid (20:4 Delta-5,8,11,14) and eicosapentaenoic acid (20:5 Delta-5,8,11,14,17), respectively. Here, we demonstrate that these conifer oil-derived PUFAs are metabolized to essential fatty acids in animal cells. When Swiss 3T3 cells were cultured with sciadonic acid, linoleic acid (18:2 Delta-9,12) accumulated in the cells to an extent dependent on the concentration of sciadonic acid. At the same time, a small amount of 16:2 Delta-7,10 appeared in the cellular lipids. Both 16:2 Delta-7,10 and linoleic acid accumulated in sciadonic acid-supplemented CHO cells, but not in peroxisome-deficient CHO cells. We confirmed that 16:2 Delta-7,10 was effectively elongated to linoleic acid in rat liver microsomes. These results indicate that sciadonic acid was partially degraded to 16:2 Delta-7,10 by two cycles of beta-oxidation in peroxisomes, then elongated to linoleic acid in microsomes. Supplementation of Swiss 3T3 cells with juniperonic acid, an n-3 analogue of sciadonic acid, induced accumulation of alpha-linolenic acid (18:3 Delta-9,12,15) in cellular lipids, suggesting that juniperonic acid was metabolized in a similar manner to sciadonic acid. This PUFA remodeling is thought to be a process that converts unsuitable fatty acids into essential fatty acids required by animals.
(キーワード): Animals / Arachidonic Acids / Fatty Acids, Essential / Fatty Acids, Unsaturated / Linoleic Acid / Metabolic Networks and Pathways / Mice / Microsomes, Liver / Peroxisomes / Rats / Swiss 3T3 Cells / alpha-Linolenic Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1742-4658.2007.05807.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17451430; ● Search Scopus @ Elsevier (PMID): 17451430; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1742-4658.2007.05807.x

(DOI: 10.1111/j.1742-4658.2007.05807.x, PubMed: 17451430) T. Yamaguchi, N. Omatsu, E. Morimoto, H. Nakashima and Tamotsu Tanaka :
CGI-58 facilitates lipolysis on lipid droplets but is not involved in the vesiculation of lipid droplets caused by hormonal stimulation,
Journal of Lipid Research, Vol.48, No.5, 1078-1089, 2007.

(要約): A lipid droplet (LD)-associated protein, perilipin, is a critical regulator of lipolysis in adipocytes. We previously showed that Comparative Gene Identification-58 (CGI-58), a product of the causal gene of Chanarin-Dorfman syndrome, interacts with perilipin on LDs. In this study, we investigated the function of CGI-58 using RNA interference. Notably, CGI-58 knockdown caused an abnormal accumulation of LDs in both 3T3-L1 preadipocytes and Hepa1 hepatoma cells. CGI-58 knockdown did not influence the differentiation of 3T3-L1 adipocytes but reduced the activity of both basal and cAMP-dependent protein kinase-stimulated lipolysis. In vitro studies showed that CGI-58 itself does not have lipase/esterase activity, but it enhanced the activity of adipose triglyceride lipase. Upon lipolytic stimulation, endogenous CGI-58 was rapidly dispersed from LDs into the cytosol along with small particulate structures. This shift in localization depends on the phosphorylation of perilipin, because phosphorylated perilipin lost the ability to bind CGI-58. During lipolytic activation, LDs in adipocytes vesiculate into micro-LDs. Using coherent anti-Stokes Raman scattering microscopy, we pursued the formation of micro-LDs in single cells, which seemed to occur in cytoplasmic regions distant from the large central LDs. CGI-58 is not required for this process. Thus, CGI-58 facilitates lipolysis in cooperation with perilipin and other factors, including lipases.
(キーワード): 1-Acylglycerol-3-Phosphate O-Acyltransferase / 3T3-L1 Cells / Adipocytes / Animals / Carcinoma, Hepatocellular / Carrier Proteins / Cell Differentiation / Cyclic AMP-Dependent Protein Kinases / Enzyme Activation / Esterases / Hormones / Lipase / Lipid Metabolism / Lipolysis / Membrane Proteins / Mice / Phosphoproteins / Phosphorylation / RNA Interference / Rats
(出版サイトへのリンク): ● Publication site (DOI): 10.1194/jlr.M600493-JLR200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17308334; ● Search Scopus @ Elsevier (PMID): 17308334; ● Search Scopus @ Elsevier (DOI): 10.1194/jlr.M600493-JLR200

(DOI: 10.1194/jlr.M600493-JLR200, PubMed: 17308334) Junichi Morishige, Kanako Touchika, Tamotsu Tanaka, Kiyoshi Satouchi, Kenji Fukuzawa and Akira Tokumura :
Production of bioactive lysophosphatidic acid by lysophospholipase D in hen egg white,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1771, No.4, 491-499, 2007.

(要約): Lysophosphatidic acid (LPA), a lysophospholipid mediator, is produced extracellularly by lysophospholipase D (lysoPLD) secreted in several animal body fluids including blood plasma. Previously, we reported that hen egg white contains polyunsaturated fatty acid-rich LPA. In this study, we examined whether lysoPLD is involved in the production of LPA in hen egg white. LysoPLD activity was measured by determining LPA and choline by mass spectrometric and enzyme-linked fluorometric analyses, respectively. LysoPLD increased with increased dilution of egg white, indicating that one or more components of egg white strongly inhibit its lysoPLD activity. This dilution-dependent increase in the lysoPLD activity was masked by co-incubation of the egg white with lysozyme, a major protein in hen egg white. Furthermore, addition of Zn(2+), Mn(2+), Ni(2+), or Co(2+) to diluted egg white altered preference patterns of lysoPLD toward choline-containing substrates. In particular, the egg white lysoPLD activity was greatly increased when Co(2+) was added. The cation-requirement of lysoPLD activity in hen egg white resembled that of plasma autotaxin (ATX)/lysoPLD. Western blot analysis revealed that egg white contained a protein that was immunostained with anti-ATX antibody. These results suggested that LPA in hen egg white is produced from lysophospholipids, especially LPC, by the action of ATX/lysoPLD, possibly originating from hen oviduct fluid.
(キーワード): Animals / Avian Proteins / Chickens / Choline / Egg White / Lysophospholipids / Metals / Phosphoric Diester Hydrolases / Substrate Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2007.01.005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17321793; ● Search Scopus @ Elsevier (PMID): 17321793; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2007.01.005

(DOI: 10.1016/j.bbalip.2007.01.005, PubMed: 17321793) Kazuma Yoshizumi, Kaoru Hirano, Hidehiro Ando, Tamotsu Tanaka and Junji Terao :
Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase,
Journal of Agricultural and Food Chemistry, Vol.54, No.2, 335-341, 2006.

(要約): Three known saponins, chiisanoside, 11-deoxyisochiisanoside, and isochiisanoside, and one novel saponin, 3,4-seco-4(23),20(29)-lupadiene-3,28-dioic acid 28-O-alpha-l-rhamnopyranosyl (1-->4)-beta-d-glucopyranosyl (1-->6)-beta-d-glucopyranoside, referred to as sessiloside, were isolated from a hot water extract of Acanthopanax sessiliflorus leaves. All of these saponins were lupane-type triterpene triglycosides, and their concentrations were 4.1, 1.0, 0.5, and 0.4% (w/w) of the total extract, respectively. Sessiloside and chiisanoside inhibited pancreatic lipase activity in vitro, and addition of the saponin-rich fraction to a high-fat diet suppressed the body weight gain of mice. The possibility of application of the lupane-type saponins from A. sessiliflorus leaves to the treatment of obesity is discussed.
(キーワード): Acanthopanax / Animals / Chromatography, High Pressure Liquid / Dietary Fats / Enzyme Inhibitors / Female / Hemolysis / Hot Temperature / Lipase / Mice / Mice, Inbred ICR / Pancreas / Plant Extracts / Plant Leaves / Rats / Saponins / Triterpenes / Water / Weight Gain
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/jf052047f
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16417288; ● Summary page in Scopus @ Elsevier: 2-s2.0-33644501134

(DOI: 10.1021/jf052047f, PubMed: 16417288, Elsevier: Scopus) J. Morishige, Y. Takai, K. Hirano, Tamotsu Tanaka and K. Satouchi :
Production and protein kinase C activation of diacylglycerols with polymethylene-interrupted PUFA residues,
Lipids, Vol.40, No.2, 155-162, 2005.

(要約): Sciadonic acid (20:3, delta-5c,11 c,14c) is a polymethylene-interrupted PUFA (PMI-PUFA) that is present in conifer seeds and known to be incorporated into animal cells and to accumulate in membrane PI as a substitute for arachidonate. In this study, we investigated whether PI having sciadonate could serve as source of DAG that could activate protein kinase C (PKC). When Swiss 3T3 cells cultured with sciadonic acid were stimulated with 100 nM of bombesin, 1-stearoyl-2-sciadonoyl-glycerol (G) and 1-stearoyl-2-arachidonoyl-G were produced. The net increments of these two molecular species of DAG reflected the levels of the two molecular species in the PI in the cells. When cells cultured with juniperonic acid (20:4, delta-5c,11c,14c,17c) were stimulated, 1-stearoyl-2-juniperonoyl-G was produced in proportion to the level of this molecular species in PI in the cells. We also examined PKC activation by synthetic DAG using a partially purified PKC fraction from rat brain and found that both 1-stearoyl-2-sciadonoyl-G and 1-stearoyl-2-juniperonoyl-G could activate PKC comparably to 1 -stearoyl-2-arachidonoyl-G. These results indicate that 1-stearoyl-PI having these C20 PMI-PUFA residues can serve as sources of potential signaling molecules.
(キーワード): Animals / Arachidonic Acids / Bombesin / Brain / Diglycerides / Enzyme Activation / Fatty Acids, Unsaturated / Male / Mice / Protein Kinase C / Rats / Signal Transduction / Swiss 3T3 Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11745-005-1370-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15884763; ● Search Scopus @ Elsevier (PMID): 15884763; ● Search Scopus @ Elsevier (DOI): 10.1007/s11745-005-1370-8

(DOI: 10.1007/s11745-005-1370-8, PubMed: 15884763) Tamotsu Tanaka, Hideki Tsutsui, Kaoru Hirano, Tohru Koike, Akira Tokumura and Kiyoshi Satouchi :
Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate-capture molecule,
Journal of Lipid Research, Vol.45, No.11, 2145-2150, 2004.

(要約): Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in wound healing, embryonic development, and progression of cancer. Here, we report a procedure for the quantification of LPA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The method is based on a characteristic mass shift with total charge change (from -2 to +1) of the phosphate species due to 1:1 complexation of LPA(2-) with a dinuclear zinc (II) complex [1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato dizinc(II) complex; Zn(2)L(3+)] at physiological pH. The monocationic complex [LPA(2-)-Zn(2)L(3+)](+) was detected in the positive mode, in which no other signal of cation adducts of LPA(2-) was observed. The detection limit of 18:1 LPA by this method was 0.1 pmol on a sample plate. The intensity ratio of [LPA(2-)-Zn(2)L(3+)](+) against an internal standard [17:0 LPA(2-)-Zn(2)L(3+)](+) increased linearly with their molar ratio. Based on the relative intensities of complex ions, we determined the amounts of LPA homologs in an egg white by this method; the results obtained were in good agreement with those by gas liquid chromatography. This sensitive and convenient procedure for LPA-specific detection is useful for the quantification of LPA homologs occurring in biological materials.
(キーワード): Animals / Cattle / Chromatography, Thin Layer / Disease Progression / Dose-Response Relationship, Drug / Egg White / Lysophospholipids / 質量分析法 (mass spectrometry) / Models, Chemical / Phosphates / Plant Proteins / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Time Factors / Wound Healing / 亜鉛 (zinc)
(出版サイトへのリンク): ● Publication site (DOI): 10.1194/jlr.D400010-JLR200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15314093; ● Summary page in Scopus @ Elsevier: 2-s2.0-17144406220

(DOI: 10.1194/jlr.D400010-JLR200, PubMed: 15314093, Elsevier: Scopus) K. Hirano, H. Matsui, Tamotsu Tanaka, F. Matsuura and K. Satouchi :
Production of 1,2-didocosahexaenoyl phosphatidylcholine by bonito muscle lysophosphatidylcholine/transacylase,
The Journal of Biochemistry, Vol.136, No.4, 477-483, 2004.

(要約): 1,2-Didocosahexaenoyl phosphatidylcholine (PC), which has highly unsaturated fatty acid at both sn-1 and sn-2 positions of glycerol, is a characteristic molecular species of bonito muscle. To examine the involvement of a de novo route in its synthesis, the molecular species of phosphatidic acid (PA) were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a 1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato dizinc(II) complex, a novel phosphate-capture molecule. However, 1,2-didocosahexaenoyl species could not be detected. Next, 1,2-didocosahexaenoyl PC synthesis by the cytosolic lysophosphatidylcholine (LPC)/transacylase was examined using endogenous LPC from bonito muscle, in which the 2-docosahexaenoyl species is abundant. The LPC/transacylase synthesized 1,2-didocosahexaenoyl PC as the most abundant molecular species. For further characterization, the LPC/transacylase was purified to homogeneity from the 100,000 x g supernatant of bonito muscle. The isolated LPC/transacylase is a labile glycoprotein with molecular mass of 52 kDa including a 5-kDa sugar moiety. The LPC/transacylase showed a PC synthesis (transacylase activity) below and above the critical micelle concentration of substrate LPC, and fatty acid release (lysophospholipase activity) was always smaller than the transacylase activity, even with a monomeric substrate. These results suggest that the LPC/transacylase is responsible for the synthesis of 1,2-didocosahexaenoyl PC.
(キーワード): Acyltransferases / Animals / Chromatography / Chromatography, High Pressure Liquid / Cytosol / Dose-Response Relationship, Drug / Fatty Acids / Fishes / Lipids / Lysophosphatidylcholines / Lysophospholipase / Micelles / Models, Chemical / Multienzyme Complexes / Muscles / Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Phosphatidic Acids / Phosphatidylcholines / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Time Factors / Zinc
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvh145
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15625317; ● Search Scopus @ Elsevier (PMID): 15625317; ● Search Scopus @ Elsevier (DOI): 10.1093/jb/mvh145

(DOI: 10.1093/jb/mvh145, PubMed: 15625317) Tamotsu Tanaka, D. Iwawaki, M. Sakamoto, Y. Takai and K. Satouchi :
Mechanisms of accumulation of arachidonate in phosphatidylinositol in yellowtail: A comparative study of acylation systems of phospholipids in rat,
European Journal of Biochemistry, Vol.270, No.7, 1466-1473, 2003.

(要約): It is known that phosphatidylinositol (PtdIns) contains abundant arachidonate and is composed mainly of 1-stearoyl-2-arachidonoyl species in mammals. We investigated if this characteristic of PtdIns applies to the PtdIns from yellowtail (Seriola quinqueradiata), a marine fish. In common with phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn) and phosphatidylserine (PtdSer) from brain, heart, liver, spleen, kidney and ovary, the predominant polyunsaturated fatty acid was docosahexaenoic acid, and levels of arachidonic acid were less than 4.5% (PtdCho), 7.5% (PtdEtn) and 3.0% (PtdSer) in these tissues. In striking contrast, arachidonic acid made up 17.6%, 31.8%, 27.8%, 26.1%, 25.4% and 33.5% of the fatty acid composition of PtdIns from brain, heart, liver, spleen, kidney and ovary, respectively. The most abundant molecular species of PtdIns in all these tissues was 1-stearoyl-2-arachidonoyl. Assay of acyltransferase in liver microsomes of yellowtail showed that arachidonic acid was incorporated into PtdIns more effectively than docosahexaenoic acid and that the latter inhibited incorporation of arachidonic acid into PtdCho without inhibiting the utilization of arachidonic acid for PtdIns. This effect of docosahexaenoic acid was not observed in similar experiments using rat liver microsomes and is thought to contribute to the exclusive utilization of arachidonic acid for acylation to PtdIns in yellowtail. Inositolphospholipids and their hydrolysates are known to act as signaling molecules in cells. The conserved hydrophobic structure of PtdIns (the 1-stearoyl-2-arachidonoyl moiety) may have physiological significance not only in mammals but also in fish.
(キーワード): Acylation / Animals / Arachidonic Acid / Brain / Brain Chemistry / Docosahexaenoic Acids / Fatty Acids / Female / Fishes / Kidney / Liver / Myocardium / Organ Specificity / Ovary / Phosphatidylinositols / Phospholipids / Rats / Spleen
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.1432-1033.2003.03512.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12654002; ● Search Scopus @ Elsevier (PMID): 12654002; ● Search Scopus @ Elsevier (DOI): 10.1046/j.1432-1033.2003.03512.x

(DOI: 10.1046/j.1432-1033.2003.03512.x, PubMed: 12654002) K. Satouchi, Y. Kodama, K. Hirano, Tamotsu Tanaka and H. Iwamoto :
A lipase-inhibiting protein from lipoxygenase-deficient soybean seeds,
Bioscience, Biotechnology, and Biochemistry, Vol.66, No.10, 2154-2160, 2002.

(要約): A lipase-inhibiting protein was isolated from lipoxygenase (LOX)-deficient soybean seeds. The molecular mass of the protein was 56.0-kDa and the N-terminal amino acid was blocked. The protein was identified by peptide mass fingerprinting in combination with matrix-assisted laser desorption ionization/time-of-flight mass spectrometry. The masses of the lysyl endopeptidase-digested peptides of the 56.0-kDa inhibiting protein were almost identical to the calculated masses of the theoretically predicted lysyl endopeptidase-treated peptides of beta-amylase from soybean seed. In a previous paper (Biosci. Biotechnol. Biochem., 62, 1498-1503, 1998), we reported that LOX-1, an isozyme of soybean seed LOX, inhibited hydrolysis of soybean oil by pancreatic lipase. Purified beta-amylase also inhibited lipase activity, although the magnitude of inhibition was weaker than that by LOX-1. Thus, there are at least two lipase-inhibiting proteins, one is a LOX and the other is a beta-amylase, in soybean seed.
(キーワード): Amino Acid Sequence / Enzyme Inhibitors / Lipase / Lipoxygenase / Molecular Sequence Data / Seeds / Soybeans / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / beta-Amylase
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.66.2154
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12450126; ● Summary page in Scopus @ Elsevier: 2-s2.0-1842837226

(DOI: 10.1271/bbb.66.2154, PubMed: 12450126, Elsevier: Scopus) Akira Tokumura, Junya Shinomiya, Seishi Kishimoto, Tamotsu Tanaka, Kentaro Kogure, Takayuki Sugiura, Kiyoshi Satouchi, Keizo Waku and Kenji Fukuzawa :
Human platelets respond differentially to lysophosphatidic acids having a highly unsaturated fatty acyl group and alkyl ether-linked lysophosphatidic acids,
The Biochemical Journal, Vol.365, No.Pt 3, 617-628, 2002.

(要約): Lysophosphatidic acid (LPA) is a physiological agonist that is produced by lysophospholipase D, phospholipase A(1) and phospholipase A(2) in the blood of animals. It exerts diverse biological actions on a broad range of animal cells. Specific receptors for this important agonist have been characterized. In this investigation, for the first time we prepared LPAs having a highly unsaturated fatty acyl group, such as the eicosapentaenoyl or docosahexaenoyl residue, and their acetylated derivatives. Human platelets aggregated more potently in response to the highly unsaturated acyl-LPAs than to LPAs with a C(18) fatty acyl group, such as an oleoyl group, while alkyl ether-linked LPAs (alkyl-LPA) had much stronger aggregating activity. Two positional isomers of LPAs with an arachidonoyl, eicosapentaenoyl or docosahexaenoyl group had equipotent aggregatory activity as well as the positional isomers of their acetylated analogues, indicating that putative LPA receptors could not distinguish the difference between the positional isomers. We found that platelet preparations from two individuals showed no aggregatory response to alkyl-LPAs, although they contained mRNAs for known LPA receptors in the following order of expression level: endothelial differentiation gene (Edg)-4>Edg-7>Edg-2. We also obtained evidence that 2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid (ONO-RS-082), a phospholipase A(2) inhibitor, potentiated alkyl-LPA-induced platelet aggregation, but inhibited highly unsaturated acyl-LPA-induced platelet aggregation. These results indicated that human platelets express acyl-LPA-selective and alkyl-LPA-selective receptors on their plasma membrane.
(キーワード): Affinity Labels / Aminobenzoic Acids / Animals / Blood Platelets / Cattle / Cinnamates / Dose-Response Relationship, Drug / Fatty Acids, Unsaturated / Gas Chromatography-Mass Spectrometry / Glyceryl Ethers / Humans / Lysophospholipids / 分子構造 (molecular structure) / Nuclear Proteins / Phosphodiesterase Inhibitors / Platelet Aggregation / Receptors, Cell Surface / Receptors, G-Protein-Coupled / Receptors, Lysophosphatidic Acid / Serum Albumin, Bovine / Transcription Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1042/BJ20020348
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11982483; ● CiNii @ 国立情報学研究所 (CRID): 1570854176748304256; ● Summary page in Scopus @ Elsevier: 2-s2.0-0036683314

(DOI: 10.1042/BJ20020348, PubMed: 11982483, CiNii: 1570854176748304256, Elsevier: Scopus) Tamotsu Tanaka, J. Morishige, T. Takimoto, Y. Takai and K. Satouchi :
Metabolic characterization of sciadonic acid (5c,11c,14c-eicosatrienoic acid) as an effective substitute for arachidonate of phosphatidylinositol,
European Journal of Biochemistry, Vol.268, No.18, 4928-4939, 2001.

(要約): Sciadonic acid (20:3 Delta-5,11,14) is an n-6 series trienoic acid that lacks the Delta8 double bond of arachidonic acid. This fatty acid is not converted to arachidonic acid in higher animals. In this study, we characterized the metabolic behavior of sciadonic acid in the process of acylation to phospholipid of HepG2 cells. One of the characteristics of fatty acid compositions of phospholipids in sciadonic acid-supplemented cells is a higher proportion of sciadonic acid in phosphatidylinositol (PtdIns) (27.4%) than in phosphatidylethanolamine (PtdEtn) (23.2%), phosphatidylcholine (PtdCho) (17.3%) and phosphatidylserine (PtdSer) (20.1%). Similarly, the proportion of arachidonic acid was higher in PtdIns (35.8%) than in PtdEtn (29.1%), PtdSer (18.2%) and PtdCho (20.2%) in arachidonic-acid-supplemented cells. The extensive accumulation of sciadonic acid in PtdIns resulted in the enrichment of newly formed 1-stearoyl-2-sciadonoyl molecular species (38%) in PtdIns and caused the reduction in the level of pre-existing arachidonic-acid-containing molecular species. The kinetics of incorporation of sciadonic acid to PtdEtn, PtdSer and PtdIns of cells were similar to those of arachidonic acid. In contrast to sciadonic acid, neither eicosapentaenoic acid (20:5 Delta-5,8,11,14,17) nor juniperonic acid (20:4 Delta-5,11,14,17) accumulated in the PtdIns fraction. Rather, these n-3 series polyunsaturated fatty acids, once incorporated into PtdIns, tended to be excluded from PtdIns. In addition, the level of arachidonic-acid-containing PtdIns molecular species remained unchanged by eicosapentaenoic-acid-supplementation. These results suggest that sciadonic acid or sciadonic-acid-containing glycerides are metabolized in a similar manner to arachidonic acid or arachidonic-acid-containing glyceride in the biosynthesis of PtdIns and that sciadonic acid can effectively modify the molecular species composition of PtdIns in HepG2 cells. In this regard, sciadonic acid will be an interesting experimental tool to clarify the significance of arachidonic acid-residue of PtdIns-origin bioactive lipids.
(キーワード): Acyltransferases / Animals / Arachidonic Acid / Arachidonic Acids / Chromatography, High Pressure Liquid / Eicosapentaenoic Acid / Fatty Acid Desaturases / Fatty Acids, Unsaturated / Humans / Hydrogenation / Kinetics / Lysophospholipids / Microsomes, Liver / Phosphatidylinositols / Rats / Substrate Specificity / Tumor Cells, Cultured
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.0014-2956.2001.02423.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11559362; ● Search Scopus @ Elsevier (PMID): 11559362; ● Search Scopus @ Elsevier (DOI): 10.1046/j.0014-2956.2001.02423.x

(DOI: 10.1046/j.0014-2956.2001.02423.x, PubMed: 11559362) S. Nakane, Tamotsu Tanaka, K. Satouchi, Y. Kobayashi and T. Sugiura :
Occurrence of a novel cannabimimetic molecule 2-sciadonoylglycerol (2-eicosa-5',11',14'-trienoylglycerol) in the umbrella pine Sciadopitys veticillata seeds,
Biological & Pharmaceutical Bulletin, Vol.23, No.6, 758-761, 2000.

(要約): The umbrella pine Sciadopitys verticillata seeds were found to contain a substantial amount (16.7 nmol/g) of sciadonic acid (all-cis-5,11,14-eicosatrienoic acid)-containing 2-monoacylglycerol, i.e., 2-sciadonoylglycerol (2-eicosa-5',11',14'-trienoylglycerol). Because the structure of 2-sciadonoylglycerol closely resembles that of 2-arachidonoylglycerol, the endogenous natural ligand for the cannabinoid receptor, we examined whether or not 2-sciadonoylglycerol exhibits cannabimimetic activity using NG108-15 neuroblastomaxglioma hybrid cells which express the cannabinoid CB1 receptor. We found that 2-sciadonoylglycerol induces rapid transient elevation of intracellular free Ca2+ concentration in NG108-15 cells through a cannabinoid CBI receptor-dependent mechanism similar to the case of 2-arachidonoylglycerol, yet the activity of 2-sciadonoylglycerol was apparently lower than that of 2-arachidonoylglycerol. The activity of 2-sciadonoylglycerol was detectable from 3-10 nM, reaching a maximum at around 10 microM. To our knowledge, this is the first report showing the occurrence of a cannabimimetic monoacylglycerol in higher plants.
(キーワード): Arachidonic Acid / Arachidonic Acids / Cannabis / Gas Chromatography-Mass Spectrometry / Glycerides / Molecular Mimicry / Seeds / Trees / Tumor Cells, Cultured
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10864031; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033945077

(PubMed: 10864031, Elsevier: Scopus) K. Hirano, E. Okada, Tamotsu Tanaka and K. Satouchi :
Purification and regiospecificity of multiple enzyme activities of phospholipase A1 from bonito muscle,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1483, No.3, 325-333, 2000.

(要約): Phospholipase A(1) (PLA(1)), which catalyzes the hydrolysis of the sn-1 ester bond of diacyl phospholipids, was purified from 100,000 x g supernatant of bonito muscle to homogeneity by ammonium-sulfate precipitation and four consecutive column chromatographies (DEAE anion-exchange, ether-Toyopeal, hydroxylapatite and Toyopeal HW 50S columns). The final preparation showed a single band above the 67-kDa molecular marker on SDS-PAGE, and the molecular mass was determined to be 71.5 kDa by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using bovine serum albumin as a standard for calibration. The N-terminal 8 amino residues were determined to be Ala-Pro-Ala-Glu-Lys-Val-Lys-Try. Regiospecificity of multiple enzyme activities of the PLA(1) was examined using positionally defined synthetic phosphatidylcholine (PC) and lysophosphatidylcholines (LPC). An acyl ester bond at the sn-1 position of PC was exclusively hydrolyzed by phospholipase activity, and 1-acyl LPC was cleaved to fatty acid and glycerophosphocholine by lysophospholipase (LPL) activity. However, the positional isomer, 2-acyl LPC was a poor substrate for LPL activity. PC/transacylation activity was also observed when excess 2-acyl LPC was supplied in the reaction mixture, and fatty acid at the sn-1 position of donor PC was transferred to the sn-1 position of acceptor LPC. These results demonstrate that the multiple enzyme activities of PLA(1), this is lysophospholipase, transacylase as well as phospholipase, have a strict regiospecificity at the sn-1 position of substrates.
(キーワード): Acyltransferases / Animals / Electrophoresis, Polyacrylamide Gel / Fishes / Lysophosphatidylcholines / Lysophospholipase / Mass Spectrometry / Molecular Weight / Multienzyme Complexes / Muscles / Phospholipases A / Substrate Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S1388-1981(99)00190-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10666567; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033956675

(DOI: 10.1016/S1388-1981(99)00190-0, PubMed: 10666567, Elsevier: Scopus) Tamotsu Tanaka, S. Izuwa, K. Tanaka, D. Yamamoto and K. Satouchi :
Non-methylene-interrupted polyunsaturated fatty acids: Effective substitute for arachidonate of phosphatidylinositol,
Biochemical and Biophysical Research Communications, Vol.264, No.3, 683-688, 1999.

(要約): In mammalian tissues and cells, a characteristic of phosphatidylinositol (PI) is a high abundance of arachidonic acid (AA) relative to the other phospholipids. In this study, we investigated the effects of supplementation of several polyunsaturated fatty acids (PUFAs) on the AA concentration of the PI fraction using a cultured cell system. Neither alpha-linolenic acid nor eicosapentaenoic acid supplement reduced the level of AA in PI of HepG2 cells. In contrast to the n-3 series PUFAs, adding podocarpic acid (20:3, Delta-5,11,14) and pinolenic acid (18:3, Delta-5,9,12) reduced the AA content of the PI fraction from a control value of 15.9% to 7.0 and 8.7%, respectively. In the experiments with pinolenic acid, selective and significant accumulation of 20:3 (Delta-7,11,14), the chain-elongated metabolite of pinolenic acid, was observed in the PI fraction. On the other hand, adding columbinic acid (18:3, Delta-5t,9,12) had no effect on AA content of the PI fraction. Because both podocarpic acid and pinolenic acid are non-methylene-interrupted fatty acids (NMIFAs) that are not converted to AA metabolically, these NMIFAs may be interesting experimental tools for research on the function of PI-origin bioactive lipids.
(キーワード): Arachidonic Acid / Fatty Acids, Unsaturated / Humans / Mass Spectrometry / Phosphatidylinositols / Tumor Cells, Cultured
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.1999.1559
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10543992; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033517775

(DOI: 10.1006/bbrc.1999.1559, PubMed: 10543992, Elsevier: Scopus) Tamotsu Tanaka, S. Izuwa, K. Tanaka, D. Yamamoto and K. Satouchi :
Biosynthesis of 1,2-dieicosapentaenoyl-sn-glycero-3-phosphocholine in Caenorhabditis elegans,
European Journal of Biochemistry, Vol.263, No.1, 189-194, 1999.

(要約): Previously, we showed that lowering the growth temperature increased the level of eicosapentaenoic acid (EPA) in the phosphatidylcholine (PtdCho) of Caenorhabditis elegans. In this study, we investigated the molecular species composition of PtdCho of C. elegans, with an emphasis on EPA-containing species. C. elegans contained a substantial amount of 1,2-dipolyunsaturated fatty acid-containing PtdCho (1,2-diPUFA-PtdCho) species, such as arachidonic acid/EPA and EPA/EPA, which are unusual phospholipids in higher animals. The EPA/EPA-PtdCho content was significantly increased in C. elegans grown at a low temperature. To examine the possibility that the acyltransferase activity involved in the remodeling of phospholipids accounts for the production of 1,2-diPUFA-PtdCho, we investigated the substrate specificity of this enzyme in C. elegans and found that it did not exhibit a preference for saturated fatty acid for acylation to the sn-1 position of PtdCho. The efficacy of the esterification of EPA to the sn-1 position was almost equal to that of stearic acid. The lack of preference for a saturated fatty acid for acylation to the sn-1 position of PtdCho is thought to result in the existence of the unusual 1,2-diEPA-PtdCho in C. elegans.
(キーワード): Acylation / Acyltransferases / Animals / Caenorhabditis elegans / Coenzyme A Ligases / Fatty Acids / Glycerylphosphorylcholine / Phosphatidylcholines / Phosphatidylethanolamines / Substrate Specificity / Temperature
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.1432-1327.1999.00480.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10429203; ● CiNii @ 国立情報学研究所 (CRID): 1573387451530325376; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033168875

(DOI: 10.1046/j.1432-1327.1999.00480.x, PubMed: 10429203, CiNii: 1573387451530325376, Elsevier: Scopus) Tamotsu Tanaka, T. Hattori, M. Kouchi, K. Hirano and K. Satouchi :
Methylene-interrupted double bond in polyunsaturated fatty acid is essential structure for metabolism by fatty acid chain elongation system of rat liver,
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol.1393, No.2-3, 299-306, 1998.

(要約): Some plant oils contain non-methylene-interrupted polyunsaturated fatty acids (NMIFAs). Pinolenic acid (all cis delta-5,9,12/18:3) and columbinic acid (trans,cis,cis delta-5,9,12/18:3) are NMIFAs that exist in pine seed oil and columbine seed oil, respectively. We investigated the double bond position of fatty acid recognized by the fatty acid chain elongation system (FACES) of rat liver using NMIFAs as experimental tools. In the total elongation assay, amounts of C2 unit chain-elongated metabolites of pinolenic acid and columbinic acid were 32% and 11%, respectively, compared to that of gamma-linolenic (all cis delta-6,9,12/18:3) as the substrate. In the condensation reaction assay, the rate limiting step of FACES, the conversion rates of pinolenic acid and columbinic acid to the corresponding C20 beta-keto fatty acids were 19% and 9% of that of gamma-linolenic acid, respectively. The formation of elongated metabolite of podocarpic acid (all cis delta-5,11,14/20:3) was only 7% of that of arachidonic acid (all cis delta-5,8,11,14/20:4). From these results it was concluded that the condensing enzyme of FACES could recognize the methylene-interrupted cis double bond structure vicinal to the carboxyl group in the fatty acid molecule.
(キーワード): Acetyltransferases / Animals / Arachidonic Acid / Fatty Acids, Unsaturated / Hydrocarbons / Liver / Male / Methane / Phospholipids / Rats / Rats, Sprague-Dawley / Triglycerides / gamma-Linolenic Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0005-2760(98)00084-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9748638; ● Search Scopus @ Elsevier (PMID): 9748638; ● Search Scopus @ Elsevier (DOI): 10.1016/S0005-2760(98)00084-8

(DOI: 10.1016/S0005-2760(98)00084-8, PubMed: 9748638) K. Hirano, H. Ito, H. Morihara, Tamotsu Tanaka and K. Satouchi :
Cytosolic lysophosphatidylcholine/transacylase in the production of dipolyunsaturated phosphatidylcholine in bonito muscle,
FEBS Lett., Vol.437, 193-196, 1998. K. Satouchi, K. Hirano, O. Fijino, M. Ikoma and Tamotsu Tanaka :
Lipoxygenase-1 from soybean seed inhibiting the activity of pancreatic lipase,
Biosci. Biotech. Biochem., Vol.62, 1498-1503, 1998. K. Hirano, A. Tanaka, K. Yoshizumi, Tamotsu Tanaka and K. Satouchi :
Properties of phospholipase A1/transacylase in the white muscle of Bonito Euthynnus pelamis (Linnaeus),
The Journal of Biochemistry, Vol.122, No.6, 1160-1166, 1997.

(要約): The properties of phospholipase A1 (PLA1) obtained from the white muscle of bonito, Euthynnus pelamis (Linnaeus), were examined. The PLA1 activity had a pH optimum from 6.5 to 7.0 for phosphatidylcholine (PC), and calcium ion was not required. The optimum temperature was from 20 to 30 degrees C. When a fatty alcohol was used as an acceptor, a wax ester was produced by transferring a fatty acid at the sn-1 position of the donor's PC. The maximum production of lysophosphatidylcholine was shifted by 0.5 pH units to the acidic side and the pH optimum of wax ester synthesis was from 6.0 to 6.5. The synthesis was independent of calcium ion and Coenzyme A. The transacylation was also observed when 1-lyso-2-acyl-sn-glycero-3-phosphocholine was used as an acceptor. Fatty acid at the sn-1 position of the donor PC was transferred to the unoccupied hydroxy group of the acceptor at the sn-1 position. When 2,3-dipalmitoyl-sn-glycero-1-phosphocholine was used as the acyl donor, a similar amount of palmitic acid was transferred as in the case of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine. However, 1-acyl-2-lyso-sn -glycero-3-phosphocholine, a positional isomer, was a poor acceptor. These results indicate that the transacylation by the PLA1 from bonito muscle is not stereospecific, but is position-specific both for the acyl donor and acceptor.
(キーワード): Acyltransferases / Animals / Fishes / Lysophosphatidylcholines / Muscles / Phosphatidylcholines / Phospholipases A / Phospholipases A1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9498560; ● Search Scopus @ Elsevier (PMID): 9498560

(PubMed: 9498560) Tamotsu Tanaka, K. Shibata, H. Hino, T. Murashita and K. Satouchi :
Purification and gas chromatographic-mass spectrometric characterization of non-methylene interrupted fatty acid incorporated in rat liver,
Journal of Chromatography. B, Biomedical Sciences and Applications, Vol.700, No.1-2, 1-8, 1997.

(要約): A C20 non-methylene interrupted trienoic acid detected in the liver of rat fed with a pine (Pinus koraiensis) seed oil diet was purified by two-step argentation thin-layer chromatography (AgTLC) and characterized by gas chromatography-mass spectrometry (GC-MS). First, a C20 methyl trienoate fraction was obtained from fatty acid methyl esters prepared from rat liver by 5% AgTLC developed with petroleum ether-diethyl ether-acetic acid (70:20:2, v/v) as a solvent system. The fraction was then subjected to AgTLC developed with benzene-acetone-diethyl ether-acetic acid (65:15:15:5, v/v) which could separate non-methylene interrupted fatty acids (NMIFA) from usual MIFAs. The purified C20 NMIFA was partially hydrogenated, and the resulting three kinds of the C20 monoenoate were analyzed by GC-MS after conversion to their dimethyl disulfide (DMDS) adducts. The results revealed that the original C20 non-methylene interrupted trienoic acid detected in the liver of rats fed with a pine seed oil diet was delta-5,11,14/20:3, a minor component of pine seed oil.
(キーワード): Animals / Chromatography, Thin Layer / Dietary Fats / Fatty Acids, Unsaturated / Gas Chromatography-Mass Spectrometry / Liver / Male / Phospholipids / Plant Oils / Rats / Rats, Sprague-Dawley / Seeds
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9390709; ● Search Scopus @ Elsevier (PMID): 9390709

(PubMed: 9390709) Tamotsu Tanaka, K. Ikita, T. Ashida, Y. Motoyama and K. Satouchi :
Effects of growth temperature on the fatty acid composition of the free-living nematode Caenorhabditis elegans,
Lipids, Vol.31, 1173-1178, 1996. Tamotsu Tanaka, Akira Tokumura and H. Tsukatani :
Platelet activating factor (PAF)-like phospholipids formed during peroxidation of phosphatidylcholines from different foodstuffs,
Bioscience, Biotechnology, and Biochemistry, Vol.59, No.8, 1389-1393, 1995.

(要約): Previously, we reported that induction of peroxidation of synthetic phosphatidylcholines (PCs) containing a polyunsaturated fatty acid by Fe(2+)-EDTA in the presence of ascorbate resulted in the formation of four types of PCs with an sn-2-oxidatively fragmented acyl group, which had platelet-aggregating activity due to interaction with platelet-activating factor (PAF) receptors. These PCs were compounds with a short-chain monocarboxylate, omega-hydroxymonocarboxylate, dicarboxylate, and dicarboxylate semialdehyde residue, respectively. In this study, we investigated the PAF-like lipids formed during peroxidation of PCs from hen egg yolk, salmon roe, sea urchin eggs, and krill in an FeSO4/EDTA/ascorbate system. The platelet-aggregating activities of these oxidized PCs were all inhibited by FR-900452, an antagonist of PAF. The activity of oxidized krill PC, which was equivalent of 89.8 +/- 8.8 pmol 16:0-PAF/mumol of starting PC, was about 5 times those of oxidized PCs from salmon roe and sea urchin eggs, and about 50 times that of oxidized hen egg yolk PC. The PAF-like phospholipids that had different combinations of long-chain alkyl or acyl groups with one of the above four types of short-chain acyl groups were identified by gas chromatography-mass spectrometry. The results indicated that foodstuffs that are rich in 1-O-alkyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine are potential sources of compounds with high PAF-like activity formed by deleterious lipid peroxidation.
(キーワード): Animals / Crustacea / Egg Yolk / Fatty Acids / Female / Food / Gas Chromatography-Mass Spectrometry / Molecular Structure / Ovary / Ovum / Oxidation-Reduction / Phosphatidylcholines / Phospholipids / Platelet Activating Factor / Salmon / Sea Urchins
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.59.1389
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7549088; ● Search Scopus @ Elsevier (PMID): 7549088; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.59.1389

(DOI: 10.1271/bbb.59.1389, PubMed: 7549088) T. Sugiura, A. Yamashita, N. Kudo, Tamotsu Tanaka and Akira Tokumura :
Platelet-activating factor and its structural analogues in the earthworm Eisenia foetida,
Biochim. Biophys. Acta, Vol.1258, 19-26, 1995. Tamotsu Tanaka, H. Iimori, H. Tsukatani and Akira Tokumura :
Platelet-aggregating effects of platelet-activating factor-like phospholipids formed by oxidation of phosphatidylcholines containing an sn-2 polyunsaturated fatty acyl group,
Biochim. Biophys. Acta, Vol.1210, 202-208, 1994. K. Satouchi, M. Sakaguchi, M. Shirakawa, K. Hirano and Tamotsu Tanaka :
Lysophosphatidylcholine from white muscle of bonito Euthynnus pelamis (Linnaeus): involvement of phospholipase A1 activity for its production,
Biochim. Biophys. Acta, Vol.1214, 303-308, 1994. Tamotsu Tanaka, H. Minamino, S. Unezaki, H. Tsukatani and Akira Tokumura :
Formation of platelet-activating factor-like phospholipids by Fe²⁺/ascorbate/EDTA-induced lipid peroxidation,
Biochim. Biophys. Acta, Vol.1210, 202-208, 1994. Akira Tokumura, Takashi Yotsumoto, T. Hoshikawa, Tamotsu Tanaka and Hiroaki Tsukatani :
Quantitative analysis of platelet-activating factor in rat brain,
Life Sciences, Vol.51, 303-308, 1992. Akira Tokumura, Tamotsu Tanaka, Takashi Yotsumoto and Hiroaki Tsukatani :
Identification of sn-2-ω-hydroxycarboxylate-containing phospholipids in a lipid extract from bovine brain,
Biochemical and Biophysical Research Communications, Vol.177, No.1, 466-473, 1991.

(要約): Phospholipids having both a long-chain acyl (palmitoyl or stearoyl) and a short-chain hydroxycarboxylyl (C3-C9) residue were identified by GC-MS in a fraction with PAF-like activity from a bovine brain lipid extract. The hydroxyl group in the hydroxycarboxylate residue was determined to be at the omega-position by comparison of the mass spectra of the tert-butyl-dimethylsilyl derivatives of these compounds with those of synthetic hydroxybutyrate-containing phosphatidylcholines. The co-existence of short-chain hydroxycarboxylate-, monocarboxylate- and dicarboxylate-containing phospholipids in the bovine brain lipid extract suggested that these compounds were formed by peroxidation of membrane phospholipids, especially phosphatidylcholines.
(キーワード): Animals / Brain Chemistry / Cattle / Gas Chromatography-Mass Spectrometry / Hydroxy Acids / 分子構造 (molecular structure) / Phospholipids / Platelet Activating Factor
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/0006-291X(91)92007-7
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 2043133; ● Summary page in Scopus @ Elsevier: 2-s2.0-0025783982

(DOI: 10.1016/0006-291X(91)92007-7, PubMed: 2043133, Elsevier: Scopus)

MISC

田中保 :
植物油脂中の非メチレン中断型不飽和脂肪酸:生理作用，構造解析および代謝,
福山大学工学部紀要, Vol.23, 111-115, 1999年.

総説・解説

田中保, 森戸克弥 :
ペルオキシソームにおける脂肪酸酸化の役割,
生化学, Vol.92, No.5, 632-639, 2020年10月.

(徳島大学機関リポジトリ): ● Metadata: 115398
(出版サイトへのリンク): ● Publication site (DOI): 10.14952/SEIKAGAKU.2020.920632
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.14952/SEIKAGAKU.2020.920632

(徳島大学機関リポジトリ: 115398, DOI: 10.14952/SEIKAGAKU.2020.920632) 田中保 :
書評 (エッセンシャル食品化学/中村宜督, 榊原啓之, 室田佳恵子編著/講談社),
生化学, Vol.91, No.5, 735, 2019年. 田中保 :
ペルオキシソームにおける脂肪酸代謝と疾患,
生化学, Vol.90, No.1, 14-20, 2018年1月.

(出版サイトへのリンク): ● Publication site (DOI): 10.14952/SEIKAGAKU.2018.900014
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572357391139840; ● Summary page in Scopus @ Elsevier: 2-s2.0-85051724025

(DOI: 10.14952/SEIKAGAKU.2018.900014, CiNii: 1520572357391139840, Elsevier: Scopus) 田中保 :
胃腸障害に効く野菜のリン脂質,
化学と生物, Vol.49, No.3, 187-192, 2011年3月.

(要約): 食べる胃腸薬といわれるキャベツやダイコン，春の七草に含まれるナズナ，スズナ，スズシロ(ダイコン)など，胃腸に良いとされる食物にアブラナ科の植物は多い．アブラナ科の植物を生で食べると，植物酵素のホスホリパーゼDと消化酵素のホスホリパーゼA2の作用で生理活性脂肪質のリゾホスファチジン酸(LPA)が生じる．消化管への野菜の効果にLPAが関与する可能性を論じる．
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/kagakutoseibutsu.49.187
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204200017792; ● Search Scopus @ Elsevier (DOI): 10.1271/kagakutoseibutsu.49.187

(DOI: 10.1271/kagakutoseibutsu.49.187, CiNii: 1390001204200017792) M. Murph, Tamotsu Tanaka, G. Mills, E. Felix and S. Liu :
Liquid Chromatography Mass Spectrometry for Quantifying Plasma Lysophospholipids: Potential Biomarkers for Cancer Diagnosis,
Methods in Enzymology, Vol.433, 1-24, 2007.

(要約): Cancer is a complex disease with many genetic and epigenetic aberrations that result in development of tumorigenic phenotypes. While many factors contribute to the etiology of cancer, emerging data implicate lysophospholipids acting through specific cell-surface, and potentially intracellular, receptors in acquiring the transformed phenotype propagated during disease. Lysophospholipids bind to and activate specific cell-surface G protein-coupled receptors (GPCRs) that initiate cell growth, proliferation, and survival pathways, and show altered expression in cancer cells. In addition, a number of enzymes that increase lysophospholipid production are elevated in particular cell lineages and cancer patients' cells, whereas in a subset of patients, the enzymes degrading lysophospholipids are decreased. Thus, ideal conditions are established to increase lysophospholipids in the tumor microenvironment. Indeed, ascites from ovarian cancer patients, which reflects both the tumor environment and a tumor-conditioned media, exhibits markedly elevated levels of specific lysophospholipids as well as one of the enzymes involved in production of lysophospholipids: autotaxin (ATX). The potential sources of lysophospholipids in the tumor microenvironment include tumor cells and stroma, such as mesothelial cells, as well as inflammatory cells and platelets activated by the proinflammatory tumor environment. If lysophospholipids diffuse from the tumor microenvironment into the bloodstream and persist, they have the potential to serve as early diagnostic markers as well as potential monitors of tumor response to therapy. Many scientific and technical challenges need to be resolved to determine whether lysophospholipids or the enzymes producing lysophospholipids alone or in combination with other markers have the potential to contribute to early diagnosis. Breast cancer is the most frequently diagnosed cancer among women. Mammography is associated with morbidity and has a high false positive and false negative rate. Thus, there is a critical need for biomarkers that can contribute to reduced false positive and false negative diagnoses, and to identify, stage, and/or predict prognosis of this disease to improve patient management. Here we describe a technical approach that can be applied to human blood plasma to measure the concentration of growth factor-like lysophospholipids contained in circulation. Using liquid chromatography mass spectrometry (LC/MS/MS), we quantified the amount of lysophosphatidic acid (16:0, 18:0, 18:1, 18:2, and 20:4), lysophosphatidylinositol (18:0), lysophosphatidylserine (18:1), lysophosphatidylcholine (16:0, 18:0, 18:1, 18:2, and 20:4), sphingosine-1-phosphate, and sphingosylphosphorylcholine species from human female plasma samples with malignant, benign, or no breast tumor present. Other methods described here include handling patient blood samples, lipid extraction, and factors that affect lysophospholipid production and loss during sample handling.
(キーワード): Blood Chemical Analysis / Breast Neoplasms / Case-Control Studies / Chromatography, Liquid / Female / Humans / Lysophospholipids / Mass Screening / Reference Standards / Tandem Mass Spectrometry / Tumor Markers, Biological
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0076-6879(07)33001-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17954226; ● Search Scopus @ Elsevier (PMID): 17954226; ● Search Scopus @ Elsevier (DOI): 10.1016/S0076-6879(07)33001-2

(DOI: 10.1016/S0076-6879(07)33001-2, PubMed: 17954226) M. Murph, Tamotsu Tanaka, S. Liu and G. Mills :
Of spiders and crabs: The emergence of lysophospholipids and their metabolic pathways as targets for therapy in cancer,
Clinical Cancer Research, Vol.12, No.22, 6598-6602, Nov. 2006.

(要約): Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P), two small lysophospholipids, are potent inducers of many of the hallmarks of cancer including cell proliferation, survival, migration, invasion, and neovascularization in in vitro and in vivo tumor models. Furthermore, the enzymes metabolizing LPA and S1P and their receptors are aberrant in multiple cancer lineages and exhibit transforming activity altering patterns and targets for metastasis. Several recent studies show the remarkable activity of new chemical genomics and/or potential novel drugs in preclinical models. Combined with the physiologic and pathophysiologic activities of LPA and S1P, these studies suggest the implementation of preclinical and clinical evaluation of LPA and S1P as therapeutic targets.
(キーワード): Animals / Drug Delivery Systems / Humans / Lysophospholipids / Metabolic Networks and Pathways / Models, Biological / Neoplasms / Receptors, Lysophosphatidic Acid / Receptors, Lysosphingolipid / Sphingosine
(出版サイトへのリンク): ● Publication site (DOI): 10.1158/1078-0432.CCR-06-1721
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17121877; ● Search Scopus @ Elsevier (PMID): 17121877; ● Search Scopus @ Elsevier (DOI): 10.1158/1078-0432.CCR-06-1721

(DOI: 10.1158/1078-0432.CCR-06-1721, PubMed: 17121877) 田中保 :
リン脂質の機能と分子種多様性の意義は?グリセロ型活性リン脂質の疎水鎖に求められる構造,
化学と生物, Vol.40, No.4, 219-221, 2002年4月. 田中保 :
ポリメチレン中断型不飽和脂肪酸 —細胞生物学的研究への有用性—,
脂質栄養学, Vol.10, No.1, 11-19, 2001年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205193167744

(CiNii: 1390001205193167744) Tamotsu Tanaka, T. Hattori, M. Kouchi, K. Hirano and K. Satouchi :
Non-methylene interrupted polyenoic acid: Structural characterization and metabolism by fatty acid chain elongation system of rat liver,
Essential Fatty acids and Eicosanoids, Invited papers from 4th International Congress, 229-233, 1998. Akira Tokumura, Tamotsu Tanaka and Hiroaki Tsukatani :
Characterization of PAF-like phospholipids formed by lipid peroxidation,
Journal of Lipid Mediators and Cell Signalling, Vol.10, 179-181, 1994. Akira Tokumura, Tamotsu Tanaka, Takashi Yotsumoto and Hiroaki Tsukatani :
Formation of PAF-like compounds by peroxidation of phospholipids from bovine brain,
Journal of Lipid Mediators, Vol.5, No.2, 127-130, 1992.

(要約): This short review describes the identification by mass spectrometry of phospholipids having an sn-2-short-chain monocarboxylate, dicarboxylate or omega-hydroxycarboxylate group in a fraction with PAF-like activity from a bovine brain lipid extract. The similar molecular heterogeneities of these unique phospholipids suggest that they are formed via a common mechanism, possibly peroxidation of membrane phosphatidylcholines. This possibility is supported by the finding that these PAF-like compounds were actually formed during peroxidation of synthetic phosphatidylcholines induced by Fe2+/ascorbate/EDTA.
(キーワード): Animals / 脳 (brain) / Cattle / 脂質過酸化 (lipid peroxidation) / 質量分析法 (mass spectrometry) / Phospholipids / Platelet Activating Factor / Platelet Aggregation
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 1525352; ● Summary page in Scopus @ Elsevier: 2-s2.0-0026659359

(PubMed: 1525352, Elsevier: Scopus)

講演・発表

Hirai Shota, Tatsuya Fukuta, Tamotsu Tanaka, Takahashi Yu, Yoshida Tatsusada and Kentaro Kogure :
Astaxanthin stereochemistry-dependent synergistic antioxidative activity of liposomes co-encapsulating with capsaicin,
The 9th Bieenial Meeting of Society for Free Radical Research (SFRR)-Asia, Apr. 2019. Kohki Tachibana, Tamotsu Tanaka, Kentaro Kogure, Tatsuhiro Ishida and Keiichiro Okuhira :
Sphingosine-1-phosphate (S1P) affects the secretion of high density lipoprotein (HDL)-constituent protein,
12th International Symposium on Nanomedicine, Ube, Dec. 2018. Kentaro Kogure, misuzu Ishikawa, shota Hirai, Tatsusada Yoshida, natsumi Shibuya, susumu Hama, yu Takahashi, Tatsuya Fukuta, Tamotsu Tanaka, shinzo hosoi and Kentaro Kogure :
Mechanism of Synergistic Antioxidative Effect of Astaxanthin and Tocotrienol by Co-encapsulated in Liposomal membranes,
The Third International Symposium on Rice Science in Global Health (ISRGH2018), Nov. 2018. Rumana Hasi Yesmin, Makoto Miyagi, Takashi Kida, Tatsuya Fukuta, Kentaro Kogure and Tamotsu Tanaka :
Amounts of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in vegetables,
The Third International Symposium on Rice Science in Global Health (ISRGH2018), Nov. 2018. Dai Majima, Ryosuke Mitsuhashi, Tatsuya Fukuta, Tamotsu Tanaka and Kentaro Kogure :
Tocopheryl succinate liposomes regulate lipid accumulation in 3T3-L1 adipocytes,
The Third International Symposium on Rice Science in Global Health (ISRGH2018), Nov. 2018. Hiroyuki Imai, Toshiki Ishikawa, Maki Kawai-Yamada, Makoto Miyagi and Tamotsu Tanaka :
Identification of phytoceramide 1-phosphate and its producing enzyme in plants,
The 23rd International Symposium on Plant Lipids, Yokohama, Jul. 2018. Tatsuya Fukuta, Tamotsu Tanaka and Kentaro Kogure :
Development of liposomes with leukocyte-like function by intermembrane transfer of leukocyte membrane proteins,
18th Symposium for GeneDesign and Delivery, Jul. 2018. yasufumi Oshima, Tatsuya Fukuta, Tamotsu Tanaka and Kentaro Kogure :
Delivery of antibody into organ and cytoplasm via faint electricity,
18th Symposium for GeneDesign and Delivery, Jul. 2018. Hinako Mori, Tatsuya Fukuta, Tamotsu Tanaka and Kentaro Kogure :
Delivery of nucleic acid medicines into pancreas by faint electricity for treatment of pancreatic diseases,
18th Symposium for GeneDesign and Delivery, Jul. 2018. Kentaro Kogure, Noriko Saito-Tarashima, K Fujikawa, Y Oshima, Tasuku Torao, M Mimura, M Hasan, S Hama, Tamotsu Tanaka, Hiroyuki Saito and Noriaki Minakawa :
Effective cellular delivery of intelligent shRNA expression device by faint electricity.,
The 5th Seminar of pharmaceutial sciences and technology., Sep. 2017. Tamotsu Tanaka, M Md Rahman, E Iga, R Yamashita, R Shimizu, K Tsuji, A Shimada, Michiyasu Nakao, Shigeki Sano and Kentaro Kogure :
Plasma level of ceramide 1-phosphate and its anti-apoptotic activity.,
58th International conference on the bioscience of lipids (ICBL), Sep. 2017. Kentaro Kogure, Noriko Saito-Tarashima, K Fujikawa, Y Oshima, Tasuku Torao, M Mimura, M Hasan, S Hama, Tamotsu Tanaka, Hiroyuki Saito and Noriaki Minakawa :
Effective cellular delivery of intelligent shRNA expression device by faint electricity.,
6th FIP Pharmaceutical Sciences World Congress (PSWC), May 2017. M Md Rahman, A Shimada, Tohru Miyazaki, K Tsuji, Michiyasu Nakao, Shigeki Sano, Kentaro Kogure and Tamotsu Tanaka :
Characterization of the Biological Effects of Ceramide-1-Phosphate.,
第58回日本生化学会中国・四国支部例会, May 2017. Kohki Fujikawa, M Hasan, S Hama, Tamotsu Tanaka and Kentaro Kogure :
Faint electric treatment induces cytoplasmic delivery of functional macromolecules via changing endosome property.,
3rd International Conference on Biomaterials Science in Tokyo (ICBS2016) (Tokyo, Japan), Nov. 2016. Yoshibumi Shimizu, Kazutoshi Kurano, Yoshiyuki Morikawa, Shigenobu Kimoto, Shinichi Okudaira, Tamotsu Tanaka, Aoki Junken, Yoshiaki Kubo and Akira Tokumura :
Potentials of the circulating mediator lysophosphatidic acid on development of pruritic dermatitis,
2013 FASEB Summer Research Conference, Lysophospholipid and other related mediators From Bench to Clinic-, Abstract, 50, Niseko, Aug. 2013. Tamotsu Tanaka, Takashi Kida, Hiroyuki Imai, Jun-ichi Morishige, Ryouhei Yamashita, Hisatsugu Matsuoka, Sachika Uozumi, Kiyoshi Satouchi, Minoru Nagano and Akira Tokumura :
Identification of a sphingolipid-specific phospholipase D activity associated with the generation of phytoceramide-1-phosphate in cabbage leaves,
2013 FASEB Summer Research Conference, Lysophospholipid and other related mediators From Bench to Clinic-, Abstract, 25, Niseko, Aug. 2013. Tamotsu Tanaka :
Bioactive phospholipids formed during digestion of plant foodstuffs and their effect on gastrointestinal integrity,
The Department of Pharmaceutical and Biomedical Sciences Research Seminar Series, Athens, Aug. 2012. Morishige Jun-ichi, Urikura Mai, Tamotsu Tanaka and Satouchi Kiyoshi :
A simple method for isolation and identification of bioactive lipids having monoester type phosphate using phosphate capture molecule, Phos-tag,
Lipid Maps Annual Meeting 2012, Lipid impact on cell biology, metabolomics and translational medicine,, Abstract-p76, La Jolla (CA, USA), May 2012. Tamotsu Tanaka, 盛重純一, 近藤宏樹, 木下正文, 足立美佳, 瓜倉真衣, 里内清 and Akira Tokumura :
Formation of lysophosphatidic acid, a wound-healing lipid, during digestion of cabbage leaves,
Keystone Symposia, Bioactive lipids: Biochemistry & Diseases, Abstract Book, 105, 京都, Jun. 2010. Mika Adachi, Jun-ichi Morishige, Tamotsu Tanaka, Junji Terao, Kiyoshi Satouchi and Akira Tokumura :
Lysophosphatidic acid in foods and herbs protects gastric ulcer in rats under water-immersion stress,
4th international conference on phospholipase A2 and lipid mediators, Vol.Final program and abstracts book, 131, Tokyo, May 2009. Jun-ichi Morishige, Tamotsu Tanaka, Tohru Koike and Kiyoshi Satouchi :
Simultaneous determination of phosphate monoester lysophospholipids using phosphate-capture molecule, Phos-tag,
4th international conference on phospholipase A2 and lipid mediators, Vol.Final program book and abstract, 131, Tokyo, May 2009. K. Yoshizumi, K. Hirano, H. Ando, Tamotsu Tanaka and J. Terao :
Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their anti-obesity activity,
Obesity reviews, Vol.7, No.(Suppl. 2), 155, Sydney, 2006. Tamotsu Tanaka, K. Hirano, T. Koike, Akira Tokumura and K. Satouchi :
Quantitative analysis of lysophosphatidic acid by matrix-assisted laser desorption time-of-flight mass spectrometry using a phosphate capture molecule,
2nd International Conference Phospholipase A2 and 8th International Congress Platelet-activating Factor and Related Lipid Mediators, 204, Berlin, Oct. 2004. Tamotsu Tanaka, T. Hattori, M. Kouchi, K. Hirano and K. Satouchi :
Chain elongation of non-methylene interrupted trienoic acid in rat liver,
Prostagalndins Leukotriens and Essential Fatty Acids, Vol.57, No.2, Edinburgh, Aug. 1997. 砂川佳吾, Md Hanif Ali, 山西百音, 小林美佑, 公門瑞希, Rumana Yesmin Hasi, 粟飯原睦美, 田中保 :
ペルオキシソーム欠損細胞における極長鎖脂肪酸毒性とオレイン酸による毒性解除,
日本農芸化学会中四国支部第67回講演会, 2024年1月. 松本尚子, 髙井誠道, 藤田智帆, MD MAJIDUL ISLAM, Rumana Yesmin Hasi, 粟飯原睦美, 田中保 :
植物におけるグリコシルイノシトールホスホセラミドとその分解酵素の解析,
日本農芸化学会中四国支部第67回講演会, 2024年1月. 髙井誠道, Rumana Yesmin Hasi, 松本尚子, 藤田智帆, MD MAJIDUL ISLAM, 粟飯原睦美, 石川寿樹, 今井博之, 田中保 :
TLCイメージングを用いた植物スフィンゴ脂質の分解経路の解析,
第96回日本生化学大会プログラム集p126, 2023年10月. Ali Hanif Md, Kobayashi Miyu, Kumon Mizuki, Yamanishi Mone, Hasi Yesmin Rumana, Mutsumi Aihara and Tamotsu Tanaka :
Effect of very long-chain fatty acids on viability of different cells,
第96回日本生化学大会プログラム集p127, Oct. 2023. 山西百音, Md Hanif Ali, 小林美佑, 公門瑞希, 粟飯原睦美, 田中保 :
可溶化した極長鎖脂肪酸の細胞への取り込みと毒性の解析,
第96回日本生化学大会プログラム集 p127, 2023年10月. Rumana Yesmin Hasi, 倭村直宏, 砂川佳吾, 髙井誠道, 松本尚子, 藤田智帆, Md Hanif Ali, MD MAJIDUL ISLAM, 石川寿樹, 梅村ゆうた, 田中秀則, 今井博之, 粟飯原睦美, 田中保 :
Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide,
第65回日本脂質生化学会, Vol.65, 237-240, 2023年6月. 北風圭介, Md Hanif Ali, 木本来希, 石丸浩靖, 竹之内康広, 山下純, 上田夏生, 田中保, 岡本安雄, 坪井一人 :
グリセロホスホジエステラーゼ7が産生する環状ホスファチジン酸はPPARγを抑制する脂質メディエーターとして機能する,
第64回日本生化学中国・四国支部例会プログラム・講演要旨集 p65, 2023年5月. Md Hanif Ali, 小林美佑, 公門瑞希, 山西百音, 粟飯原睦美, 田中保 :
極長鎖脂肪酸の可溶化と細胞への取り込み解析,
第64回日本生化学中国・四国支部例会プログラム・講演要旨集 p33, 2023年5月. 田中保, Md Hanif Ali, 小林美佑, Rumana Yesmin Hasi, 粟飯原睦美, 林順司, 川上竜巳 :
極長鎖脂肪酸による毒性とその解毒装置としてのペルオキシソームの役割,
脂質栄養学, Vol.31, No.2, 143, 2022年9月. 宇山徹, Zahir Hussain, 森戸克弥, 田中保, 太田健一, 上野正樹, 村上誠, 上田夏男 :
cPLA2eは脳障害部位でN-アシル-ホスファチジルエタノールアミンを合成する,
第64回日本脂質生化学会, Vol.64, 272-275, 2022年6月. Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Distribution and characterization of glycosylinositol phosphoceramide specific phospholipase D in Brassica plants,
第64回日本脂質生化学会, Vol.64, 272-275, Jun. 2022. Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Peroxisomes attenuate lipotoxicity of very-long-chain fatty acids,
第64回日本脂質生化学会, Vol.64, 43-46, Jun. 2022. 森戸克弥, 島田明奈, 宮崎徹, 清水良多, 高橋尚子, 東桃代, 下澤伸行, 福田達也, 小暮健太朗, 田中保 :
X連鎖性副腎白質ジストロフィー患者血漿中セラミドの分析とその主要な分子種の動物細胞への取り込みと作用,
第63回日本生化学中国・四国支部例会, 2022年5月. 北風圭介, 坪井一人, Md Hanif Ali, 木本来希, 竹之内康広, 石丸浩靖, 山下純, 上田夏男, 田中保, 岡本安雄 :
グリセロホスホジエステラーゼ7は小胞体内腔において環状ホスファチジン酸を産生する,
第63回日本生化学中国・四国支部例会, 2022年5月. Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Metabolism and biological effect of very-long-chain fatty acid in peroxisome-deficient cells,
第63回日本生化学中国・四国支部例会, May 2022. 植野美彦, 櫻谷英治, 関陽介, 上岡麻衣子, 浅田元子, 赤松徹也, 宮脇克行, 宇都義浩, 田中保 :
一般選抜後期日程における入学辞退率改善の取り組みーー徳島大学B学部の事例からーー,
第17回大学教育カンファレンスin徳島, 2022年1月. 田中保 :
植物スフィンゴ脂質およびその代謝酵素の産業的利用,
第3回脂質駆動学術産業創生研究部会講演会, 2021年12月. Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Characterization of uptake and metabolism of very-long-chain fatty acid in peroxisome-deficient CHO cells,
第94回日本生化学大会, Nov. 2021. Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Production of phytoceramide 1- phosphate and inositol glycan by glycosylinositol phosphoceramide specific phospholipase D activity in plants,
第94回日本生化学大会, Nov. 2021. Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Characterization of uptake and metabolism of very-long-chain fatty acid in peroxisome-deficient CHO cells,
第62回日本生化学中国・四国支部例会, Sep. 2021. Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Production of phytoceramide 1- phosphate and inositol glycan by glycosylinositol phosphoceramide specific phospholipase D activity in plants,
第62回日本生化学中国・四国支部例会, Sep. 2021. Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya and Tamotsu Tanaka :
Uptake and metabolism of very-long-chain fatty acid in animal cells,
第63回日本脂質生化学会, Jun. 2021. Rumana Yesmin Hasi, Yoshimichi Takai, Hanif Ali, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Mutsumi Aihara, Kaori Kanemaru and Tamotsu Tanaka :
Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate from cabbage leaves and their chemical stabilities.,
日本農芸化学会2020年度中四国支部大会(第57回講演会), Sep. 2020. 坪井一人, 田井達也, 山下量平, 宇山徹, 岡本蓉子, 郷慎司, 渡邉悦子, Iffat Sonia Ara Rahman, 芳地一, 田中保, 岡本安雄, 徳村彰, 松田純子, 上田夏生 :
脳虚血モデルでのN-アシル-ホスファチジルエタノールアミンの蓄積はcPLA2eによって引き起こされる,
第93回日本生化学大会, 2020年9月. Rumana Yesmin Hasi, Dai Majima, Katsuya Morito, Hanif Ali, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Meera Nanjundan, Toshiki Ishikawa and Tamotsu Tanaka :
Methods for isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate from plant tissues.,
第93回日本生化学大会, Sep. 2020. Rumana Yesmin Hasi, Dai Majima, Katsuya Morito, Hanif Ali, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Meera Nanjundan, Toshiki Ishikawa, Hiroyuki Imai and Tamotsu Tanaka :
Development of methods for isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate from plant tissues.,
第62回日本脂質生化学会, May 2020. 田中保, Rumana Hasi Yesmin, 森戸克弥, 小暮健太朗, 林順司, 川上竜巳, 金丸芳, 今井博之, 石川寿樹 :
グリコシルイノシトールホスホセラミドの単離法の開発,
第12回セラミド研究会学術集会, 2019年10月. 田中保, 森戸克弥, Rumana Hasi Yesmin, 林順司, 川上竜巳, 金丸芳, 若山睦, 近藤千恵子, 福田達也, 小暮健太朗 :
食品素材に含まれるセラミドの簡便な定量方法,
日本脂質栄養学会第28回大会, 2019年9月. 宇山徹, Binte Mustafiz Smriri Sultana, 森戸克弥, 高橋尚子, 川合克久, Hussain Zahir, 坪井一人, 荒木伸一, 山本圭, 田中保, 上田夏生 :
cPLA2eによるN-アシル‐ホスファチジルエタノールアミンの細胞内カルシウム依存的な生成,
第92回日本生化学会大会, 2019年9月. 堤敏彦, 松田璃沙, 森戸克弥, 横田美帆, 荷川取史妃, 川島聡, 藤原愛美, 山本武範, 山﨑尚志, 田中保, 篠原康雄, 德村彰 :
動物培養細胞においてグリセロホスホジエステラーゼ3はリゾホスファチジルイノシトールをモノアシルグリセロールに分解するエクト型リゾホスホリパーゼCとして機能する,
第92回日本生化学会大会, 2019年9月. 今井博之, 田中保, 石川寿樹, 川合真紀 :
LC-MS/MSによるフィトセラミド1-リン酸分子種の定量解析,
第92回日本生化学会大会, 2019年9月. 森戸克弥, 島田明奈, 宮崎徹, 清水良多, 高橋尚子, 下澤伸行, 東桃代, 福田達也, 小暮健太朗, 田中保 :
ヒト血漿の主要なセラミド及びセラミド1-リン酸分子種の動物細胞への取り込みと作用,
第92回日本生化学会大会, 2019年9月. 高橋尚子, 清水良多, 森戸克弥, 東桃代, 下澤伸行, 福田達也, 小暮健太朗, 田中保 :
液体クロマトグラフィー/タンデム質量分析による副腎白質ジストロフィー患者の血漿中セラミド分子種及び濃度の解析,
第92回日本生化学会, 2019年9月. 森戸克弥, 島田明奈, 宮崎徹, 清水良多, 高橋尚子, 東桃代, 下澤伸行, 西岡安彦, 福田達也, 小暮健太朗, 田中保 :
ヒト血漿中セラミド及びセラミド1-リン酸の分子種組成と動物細胞への取り込みと作用,
第61回日本脂質生化学会, 2019年7月. 道上巧基, 福田達也, 田中保, 佐藤陽一, 小暮健太朗 :
男性不妊症治療を目指した微弱電流処理による精巣への非侵襲的薬物送達技術の開発,
第35回日本DDS学会学術集会, 2019年7月. 真島大, 三橋亮介, 梶本和昭, 福田達也, 田中保, 小暮健太朗 :
トコフェロールコハク酸リポソームによる抗肥満効果の検討,
日本ビタミン学会第71回大会, 2019年6月. 髙橋尚子, 清水良多, 森戸克弥, 東桃代, 下澤伸行, 福田達也, 小暮健太朗, 田中保 :
液体クロマトグラフィー/タンデム質量分析を用いた副腎白質ジストロフィー患者の血中セラミド分析,
第60回日本生化学中国・四国支部例会, 2019年5月. Rumana Hasi Yesmin, Makoto Miyagi, Takashi Kida, Tatsuya Fukuta, Kentaro Kogure and Tamotsu Tanaka :
Development of methods for purification of plant sphingolipids, glycosylinositol phosphoceramide and phytoceramide 1-phosphate,
日本農芸化学会2019年度大会, Mar. 2019. 森戸克弥, 島田明奈, 宮﨑徹, 清水良多, 高橋尚子, 東桃代, 小山壱也, 西岡安彦, 福田達也, 小暮健太朗, 田中保 :
ヒト血漿中セラミド及びセラミド1-リン酸の分子種組成と動物細胞へ作用,
日本農芸化学会2019年度大会, 2019年3月. 平田悠真, 福田達也, 田中保, 真島英司, 小暮健太朗 :
Protein Aを用いた新規抗体修飾リポソーム調製法,
日本薬学会第139年会, 2019年3月. 吉見真太朗, 福田達也, 田中保, 小暮健太朗 :
がん親和性付与を目的とした単球膜タンパク質搭載リポソームの構築,
日本薬学会第139年会, 2019年3月. 森戸克弥, 清水良多, 北村苗穂子, 朴時範, 岸野重信, 小川順, 福田達也, 小暮健太朗, 田中保 :
乳酸菌が産生するリノール酸代謝物の動物細胞における代謝と宿主脂質代謝への影響,
第9回学際的脂質創生研究部会, 2019年2月. 真島大, 三橋亮介, 梶本和昭, 福田達也, 田中保, 小暮健太朗 :
トコフェロールコハク酸リポソームは3T3-L1脂肪細胞の脂肪蓄積を制御する,
第30回ビタミンE研究会, 2019年1月. 小暮健太朗, 石川みすず, 平井将太, 濵進, 細井信造, 吉田達貞, 髙橋侑, 福田達也, 田中保 :
トコトリエノールとアスタキサンチンの相乗的抗酸化効果メカニズム,
第30回ビタミンE研究会, 2019年1月. 小暮健太朗, 三橋亮介, 真島大, 福田達也, 田中保 :
ビタミンEコハク酸による脂肪蓄積抑制作用,
第360回脂溶性ビタミン総合委員会, 2018年12月. 立花洸季, 田中保, 小暮健太朗, 石田竜弘, 奥平桂一郎 :
HDL構成タンパク質分泌に対するスフィンゴシン-1-リン酸及びフィンゴリモドの影響,
第57回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2018年11月. 宮﨑徹, 島田明奈, 高橋尚子, Md. Motiur Rahman, 清水良多, 辻和樹, 森戸克弥, 山下量平, 佐野茂樹, 中尾允泰, 福田達也, 小暮健太朗, 田中保 :
外因的に加えた極長鎖脂肪酸およびこれを含有するセラミドのアポトーシスへの影響,
第11回セラミド研究会, 2018年10月. 田中保, 宮城諒, 藤原美奈, 辻和樹, 森戸克弥, Rumana Hasi Yesmin, 福田達也, 小暮健太朗, 今井博行, 石川寿樹, 川合真紀 :
植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼD活性の分布と性質,
第11回セラミド研究会学術集会, 2018年10月. 立花洸季, 西辻和親, 田中保, 小暮健太朗, 石田竜弘, 奥平桂一郎 :
スフィンゴシン-1-リン酸(S1P)による高密度リポプロテイン(HDL)構成タンパク質分泌への影響,
第91回日本生化学会大会, 2018年9月. 坪井一人, 井上愛美, 岡本蓉子, 日高麻由美, 宇山徹, 堤敏彦, 田中保, 岡本安雄, 上田夏生, 德村彰 :
N-アシル-ホスファチジルエタノールアミン特異的ホスホリパーゼD欠損マウスの末梢臓器における関連脂質とその代謝経路の解析,
第91回日本生化学大会, 2018年9月. 堤敏彦, 井上愛美, 岡本蓉子, 渥美祐太, 塩尻正俊, 日高麻由美, 田中保, 白坂直輝, 德村彰 :
食餌への高濃度のリゾホスファジン酸添加はマウスの体重と体脂肪を減少させる,
第91回日本生化学大会, 2018年9月. 今井博之, 田中保, 石川寿樹, 川合真紀 :
植物に存在するセラミド 1-リン酸のLC-MS/MSによる分析,
第91回日本生化学大会, 2018年9月. 立花洸季, 田中保, 小暮健太朗, 石田竜弘, 奥平桂一郎 :
HDL構成タンパク質分泌に対するスフィンゴシン-1-リン酸及びフィンゴリモドの影響,
第91回日本生化学会大会, 2018年9月. 田中保, 宮城諒, 辻和樹, 藤原美奈, 森戸克弥, 石川寿樹, 今井博之, 川合真紀, 福田達也, 小暮健太朗 :
植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性質,
日本農芸化学会2018年度中四国支部大会, 2018年9月. 森戸克弥, 清水良多, 高橋尚子, 下澤伸行, 東桃代, 河野弘, 西岡安彦, 福田達也, 小暮健太朗, 田中保 :
ヒト血漿中セラミド及びセラミド1-リン酸の分子種組成と細胞への取り込み,
第91回日本生化学会大会, 2018年9月. 宮城諒, 辻和樹, 藤原美奈, 森戸克弥, 石川寿樹, 今井博之, 川合真紀, 福田達也, 小暮健太朗, 田中保 :
植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性質,
第91回日本生化学会大会, 2018年9月. 田中保, 森戸克弥, 清水良多, 北村苗穂子, 朴時範, 岸野重信, 小川順, 福田達也, 小暮健太朗 :
腸内細菌が産生するヒドロキシ脂肪酸の動物細胞における代謝,
日本脂質栄養学会第27回大会, 2018年8月. 森日向子, 福田達也, 田中保, 小暮健太朗 :
膵臓疾患治療を目指した微弱電流による核酸医薬の膵臓内送達,
第18回遺伝子・デリバリー研究会第18回夏期セミナー, 2018年7月. 大島康史, 福田達也, 田中保, 小暮健太朗 :
微弱電流処理による抗体の細胞内・皮内デリバリー,
第18回遺伝子・デリバリー研究会第18回夏期セミナー, 2018年7月. 福田達也, 虎尾祐, 三村美夕紀, 大島康史, 中谷奈津, 田中保, 小暮健太朗 :
弱電流による特殊なエンドサイトーシスを利用した高分子送達の機構解析,
第18回遺伝子・デリバリー研究会第18回夏期セミナー, 2018年7月. 小暮健太朗, 田中太智, 森日向子, 賀川真夕子, Hasan Mahadi, 福田達也, 田中保 :
微弱電流処理による体内臓器細胞へのsiRNAの送達,
日本核酸医薬学会第4回年会, 2018年7月. 小暮健太朗, 三橋亮介, 福田達也, 田中保 :
脂肪細胞における脂肪蓄積へのトコフェロールコハク酸リポソームの影響,
日本ビタミン学会第70回大会, 2018年6月. 中谷奈津, 田中太智, 平田悠真, 森日向子, 吉見真太朗, 福田達也, 田中保, 小暮健太朗 :
微弱電流処理と活性種(NO)とを組み合わせることによる細胞内取り込みの変化,
第34回日本DDS学会学術集会, 2018年6月. 森戸克弥, 清水良多, 北村苗穂子, 朴時範, 岸野重信, 小川順, 福田達也, 小暮健太朗, 田中保 :
乳酸菌が産生するリノール酸代謝物の動物細胞への取り込みと代謝,
第59回日本生化学会中国・四国支部例会, 2018年5月. 小暮健太朗, 平井将太, 髙橋侑, 田中保, 福田達也, 吉田達貞 :
アスタキサンチンと抗酸化物質の共封入リポソームによる相乗的な抗酸化効果,
71回日本酸化ストレス学会第18回日本NO学会合同学術集会, 2018年5月. 宮﨑徹, 島田明奈, 高橋尚子, Md. Motiur Rahman, 清水良多, 辻和樹, 森戸克弥, 山下量平, 佐野茂樹, 中尾允泰, 福田達也, 小暮健太朗, 田中保 :
外因的に加えた極長鎖脂肪酸および極長鎖脂肪酸含有セラミドのアポトーシスへの影響,
第60回日本脂質生化学会, 2018年5月. 田中太智, Hasan Mahadi, 福田達也, 田中保, 小暮健太朗 :
腎臓疾患治療を目指したイオントフォレシスによる核酸医薬の腎臓内送達,
日本薬剤学会第33年会, 2018年5月. 福田達也, 田中保, 小暮健太朗 :
脂質膜間移行現象を利用したリポソームへの白血球様機能の付与,
日本膜学会40年会, 2018年5月. 虎尾祐, 三村美夕紀, 大島康史, 賀川真夕子, 藤川昂樹, 福田達也, 田中保, 小暮健太朗 :
微弱電流による特殊なエンドサイトーシスを介した体内臓器細胞への高分子送達,
日本膜学会40年会, 2018年5月. 賀川真夕子, 福田達也, 田中保, 小暮健太朗 :
イオントフォレシスによる肝臓への核酸医薬送達,
日本薬学会138年会, 2018年3月. 平井将太, 髙橋侑, 田中保, 福田達也, 吉田達貞, 小暮健太朗 :
アスタキサンチンと抗酸化物質の組合せによる相乗的な活性酸素消去活性の向上.,
日本薬学会138年会, 2018年3月. 三村美夕紀, 大島康史, 虎尾祐, 藤川昂樹, 福田達也, 田中保, 小暮健太朗 :
微弱電流処理により誘導される細胞取り込み過程の定量的評価,
日本薬学会138年会, 2018年3月. 大島康史, Hasan Mahadi, 田良島典子, 濱進, 福田達也, 田中保, 南川典昭, 小暮健太朗 :
微弱電流処理を利用した機能性核酸の細胞内取り込みの検討,
日本薬学会138年会, 2018年3月. 三橋亮介, 梶本和昭, 福田達也, 田中保, 小暮健太朗 :
トコフェロールコハク酸含有リポソームによる脂肪蓄積の抑制作用,
日本薬学会138年会, 2018年3月. 虎尾祐, 大島康史, 三村美夕紀, 藤川昂樹, 福田達也, 田中保, 小暮健太朗 :
微弱電流処理によるユニークなエンドサイトーシス誘導に関連する因子の検討,
日本薬学会138年会, 2018年3月. 森戸克弥, 清水良多, 北村苗穂子, 朴時範, 岸野重信, 小川順, 福田達也, 小暮健太朗, 田中保 :
乳酸菌が産生する希少脂肪酸のペルオキシソームにおける代謝,
日本農芸化学会 2018年度大会, 2018年3月. 三橋亮介, 福田達也, 田中保, 小暮健太朗 :
トコフェロールコハク酸含有リポソームによる脂肪細胞の脂肪蓄積抑制効果,
第29回ビタミンE研究会, 2018年1月. 小暮健太朗, 石川みすず, 平井将太, 濵進, 吉田達貞, 髙橋侑, 細井信造, 福田達也, 田中保 :
α-トコトリエノールとアスタキサンチンの相乗的抗酸化効果,
第357回脂溶性ビタミン総合研究委員会, 2017年12月. 清水良多, 山下量平, 伊賀永里奈, Md. Motiur Rahman, 東桃代, 小暮健太朗, 田中保 :
液体クロマトグラフィー/タンデム質量分析によるヒト血漿中のセラミド及びセラミド 1-リン酸の解析,
第40回日本分子生物学会年会，第90回日本生化学会大会プログラム, 439, 2017年12月. Md Motiur Rahman, Erina Iga, Akina Shimada, Tohru Miyazaki, Naoko Takahashi, Mina Fujiwara, Kazuki Tsuji, Kentaro Kogure and Tamotsu Tanaka :
Neuroprotective activity of phytoceramide 1-phosphate on serum deprivation-induced apoptosis of Neuro2a cells,
第40回日本分子生物学会年会，第90回日本生化学会大会プログラム, 439, Dec. 2017. Afroz Sheuli, Katsuya Morito, Kouki Fujikawa, Ayano Yagi, Kazunori Toida, Emi Kiyokage, Kentaro Kogure, Shiro Watanabe, Akira Tokumura and Tamotsu Tanaka :
Antiulcer effect of lysophosphatidic acid-rich medicinal herbs and its mechanism,
第40回日本分子生物学会年会，第90回日本生化学会大会プログラム, 439, Dec. 2017. 辻和樹, 島田明奈, 宮崎徹, 高橋尚子, 伊賀永里奈, Rahman Md Motiur, 中尾允泰, 佐野茂樹, 小暮健太朗, 田中保 :
種々の脂肪酸残基を有するセラミドの化学合成とその生理活性,
第40回日本分子生物学会年会，第90回日本生化学会大会プログラム, 438, 2017年12月. 小暮健太朗, 大島康史, 虎尾祐, 三村美夕紀, 藤川昂樹, Hasan Mahadi, 濱進, 福田達也, 田良島典子, 田中保, 南川典昭 :
微弱電流処理による高分子医薬の細胞質送達と機能発現,
第39回生体膜と薬物の相互作用シンポジウム, 2017年10月. 三橋亮介, 福田達也, 田中保, 小暮健太朗 :
トコフェロールコハク酸含有リポソームによる脂肪蓄積減少効果の検討,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. 虎尾祐, 大島康史, 三村美夕紀, 藤川昂樹, 福田達也, 田中保, 小暮健太朗 :
微弱電流処理によるユニークなエンドサイトーシス誘導機構の検討,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. 三村美夕紀, 大島康史, 虎尾祐, 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 小暮健太朗 :
微弱電流処理により誘起される細胞内取り込みの評価,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. 賀川真夕子, 福田達也, 田中保, 小暮健太朗 :
微弱電流処理によるsiRNAの細胞内送達と肝細胞遺伝子発現制御,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会, 2017年10月. 大島康史, 虎尾祐, 三村美夕紀, Hasan Mahadi, 田良島典子, 濱進, 福田達也, 田中保, 南川典昭, 小暮健太朗 :
ユニークなエンドサイトーシスを誘起する微弱電流を利用した機能性核酸の細胞質送達,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. 田中保, 山下量平, 清水良多, 森戸克弥, Md. Motiur Rahman, 伊賀永里奈, 島田明奈, 福田達也, 小暮健太朗 :
血液中のセラミド 1-リン酸の分子種組成と生物活性,
セラミド研究会予稿集, 23, 2017年10月. 島田明奈, 宮﨑徹, 高橋尚子, Rahman Motiur Md., 清水良多, 辻和樹, 山下量平, 佐野茂樹, 中尾允泰, 福田達也, 小暮健太朗, 田中保 :
極長鎖脂肪酸およびこれを含有するセラミドのアポトーシス抑制活性,
セラミド研究会, 2017年10月. Md Motiur Rahman, Erina Iga, Tohru Miyazaki, Naoko Takahashi, MIna Fujiwara, Kazuki Tsuji, Kentaro Kogure and Tamotsu Tanaka :
Phytoceramide 1-phosphate in vegetables and its anti-apoptotic effect in animal cells,
日本脂質栄養学会第26回大会予稿集, 187, Sep. 2017. S Afroz, Katsuya Morito, K Fujikawa, A Yagi, Teru Ikoma, E Kiyokage, K Toida, Kentaro Kogure and Tamotsu Tanaka :
Phosphatidic acid-rich cereals as anti-ulcer foods and their mechanisms of action,
日本脂質栄養学会第26回大会, Sep. 2017. 大島康史, 虎尾祐, 三村美夕紀, 藤川昂樹, Hasan Mahadi, 濱進, 福田達也, 田中保, 小暮健太朗 :
微弱電流が誘起するユニークなエンドサイトーシスによる核酸の細胞質送達,
遺伝子・デリバリー研究会第17回夏期セミナー, 2017年9月. 小暮健太朗, 賀川真夕子, 大島康史, 虎尾祐, 三村美夕紀, 福田達也, Mahadi Hasan, 濱進, 田中保 :
微弱電流による核酸医薬の細胞内送達,
第26回DDSカンファランス, 2017年9月. 虎尾祐, 三村美夕紀, 大島康史, 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 小暮健太朗 :
微弱電流処理によるユニークなエンドサイトーシスの解析,
第33回日本DDS学会学術集会, 2017年7月. 宮城諒, 喜田孝史, 辻和樹, 小暮健太朗, 田中保 :
グリコシルイノシトールホスホセラミドの抽出と精製,
日本農芸化学会中四国支部第48回公演会要旨集, 37, 2017年6月. 田中保, Md Motiur Rahaman, 伊賀永理奈, 山下量平, 清水良多, 辻和樹, 島田明奈, 中尾允泰, 佐野茂樹, 小暮健太朗 :
血液中に存在する極長鎖セラミド-1-リン酸のアポトーシス抑制作用,
第59回日本脂質生化学会, 講演要旨集, 33-34, 2017年6月. 喜田孝史, 伊藤葵, 木村朱里, 松岡久嗣, 藤原美奈, 辻和樹, 小暮健太朗, 田中保 :
植物におけるグリコシルイノシトールホスホセラミド特異的ホスホリパーゼD活性の分布と性質,
第59回日本脂質生化学会, 2017年6月. 小暮健太朗, 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 田良島典子, 南川典昭 :
微弱電流による新規核酸iRedの細胞内送達と遺伝子発現制御,
遺伝子・デリバリー研究会第17回シンポジウム, 2017年5月. 大島康史, 虎尾祐, 三村美夕紀, 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 小暮健太朗 :
微弱電流により誘起されるユニークなエンドサイトーシスの解析,
遺伝子・デリバリー研究会第17回シンポジウム, 2017年5月. 清水良多, 山下量平, 伊賀永里奈, Rahman Motiur Md., 東桃代, 小暮健太朗, 田中保 :
液体クロマトグラフィー/タンデム質量分析による血漿中のセラミド及びセラミド-1-リン酸の解析,
第58回日本生化学会中国・四国支部例会, 2017年5月. 島田明奈, 伊賀永里奈, Rahman Motiur Md., 山下量平, 清水良多, 小暮健太朗, 田中保 :
セラミド-1-リン酸のアポトーシス抑制活性,
第58回日本生化学会中国・四国支部例会, 2017年5月. 三村美夕紀, 大島康史, 虎尾祐, 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 小暮健太朗 :
微弱電流処理によって誘起されるユニークなエンドサイトーシス,
日本薬剤学会第32年会, 2017年5月. 船城凌, 渋谷菜摘, 田中保, 小暮健太朗, 奥平桂一郎 :
HepG2細胞でのスフィンゴシン1リン酸(S1P)によるアポリポタンパク質A-I(apoA-I)発現の抑制,
日本薬学会第137年会, 2017年3月. 小暮健太朗, Mahadi Hasan, 田良島典子, 藤川昂樹, 濱進, 田中保, 樫田啓, 浅沼浩之, 斎藤博幸, 南川典昭 :
微弱電流による機能性核酸の効率的な細胞質送達,
日本核酸医薬学会第2回年会(東京), 2016年12月. 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 斎藤博幸, 小暮健太朗 :
微弱電流処理による高分子物質の細胞質送達.,
第38回生体膜と薬物の相互作用シンポジウム(名古屋), 2016年11月. 屋宜亜耶乃, Afroz Sheuli, 生駒照, 德村彰, 小暮健太朗, 田中保 :
食物中のホスファチジン酸の抗胃潰瘍効果とホスホリパーゼA2活性化作用.,
第55回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会(岡山), 2016年10月. 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 小暮健太朗 :
細胞のエンドソーム物性変化を誘導する微弱電流処理.,
第55回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会(岡山), 2016年10月. 坪井一人, Iffat Sonia Ara Rahman, 岡本蓉子, 宇山徹, 山﨑尚志, 田中保, 德村彰, 上田夏生 :
GDE7はリゾホスホリパーゼD型酵素としてN-アシルエタノールアミンとLPAを生成する,
第89回日本生化学会大会, 2016年9月. 山下量平, 伊賀永里奈, 柿内直哉, 辻和樹, 小暮健太郎, 德村彰, 中尾允泰, 佐野茂樹, 田中保 :
種々のセラミド-1-リン酸分子種の生理活性とその代謝,
第58回日本脂質生化学会, 2016年6月. 松田璃沙, 坪井一人, 岡本蓉子, 山下量平, Rahman Ara Sonia Iffat, 日高麻由美, 山﨑尚志, 上田夏生, 田中保, 德村彰 :
口腔粘膜上皮細胞に存在する膜結合型リゾホスホリパーゼD,
第58回日本脂質生化学会, 2016年6月. Hasan Mahadi, Noriko Saito-Tarashima, Kohki Fujikawa, Takashi Ohgita, Susumu Hama, Tamotsu Tanaka, Hiroyuki Saito, Noriaki Minakawa and Kentaro Kogure :
Intracellular delivery of a novel functional nucleic acid iRed by faint electric treatment for effective regulation of target genes,
第32回DDS学術集会, Jun. 2016. Iffat Sonia Ara Rahman, Kazuhito Tsuboi, Yoko Okamoto, Toru Uyama, 山﨑尚志, 田中保, 德村彰, Natsuo Ueda :
Glycerophosphodiesterases, GDE4 and GDE7, are novel lysophospholipase D-type enzymes generating N-acylethanolamine and LPA,
第57回日本生化学会中国四国支部例会, 2016年5月. 藤川昂樹, Hasan Mahadi, 濱進, 田中保, 小暮健太朗 :
微弱電流処理によって誘起されるエンドサイトーシスの解析.,
日本薬剤学会第31年会(岐阜), 2016年5月. 山下量平, 柿内直哉, 伊賀永里奈, 田畑優美香, 島田明奈, 德村彰, 田中保 :
種々のマウス組織におけるセラミド-1-リン酸分子種とその代謝,
日本農芸化学会中四国支部第44回公演会要旨集, 41, 2016年1月. Sheuli Afroz, Teru Ikoma, Ayano Yagi, Shiro Watanabe, 德村彰, 田中保 :
Effect of phosphatidic acid on NSAIDs-induced stomach ulcer and its content in cereals,
日本農芸化学会中四国支部第44回公演会要旨集, 33, 2016年1月. 清水良多, 魚住幸加, 森戸克弥, 大隅隆, 德村彰, 田中保 :
脂肪酸鎖長の伸長と短縮反応におけるペルオキシソームの役割(口頭発表選出),
第88回日本生化学会大会, 2015年12月. 辻和樹, 伊藤葵, 木村朱里, 松岡久嗣, 藤原美奈, 喜田孝史, 今井博之, 德村彰, 田中保 :
植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性状と分布,
第88回日本生化学会大会, 2015年12月. 渋谷菜摘, 藤川昂樹, 田中保 :
セラミド-1-リン酸のヒト胃由来MKN74細胞に対する小胞分泌作用,
第88回日本生化学会大会, 2015年12月. 柿内直哉, 山下量平, 田畑優美香, 伊賀永里奈, 島田明奈, 辻一樹, 德村彰, 田中保 :
種々のマウス組織におけるセラミド-1-リン酸分子種とその代謝,
第88回日本生化学会大会, 2015年12月. Afroz Sheuli, 生駒照, 屋宜彩乃, Akira Tokumura and Tamotsu Tanaka :
Effect of phosphatidic acid on indomethacin-induced stomach ulcer and its content in plant sources,
第88回日本生化学会大会, Dec. 2015. 藤川昂樹, 生駒照, 森戸克弥, 清蔭恵美, 徳田一徳, 清水太郎, 石田竜弘, 德村彰, 田中保 :
ヒト胃由来培養細胞におけるリゾホスファチジン酸誘導性小胞分泌現象の解析,
第88回日本生化学会大会, 2015年12月. 岡本蓉子, 坪井一人, Sonia Ara Iffat Rahman, 上田夏生, 田中保, 德村彰 :
新規リゾホスホリパーゼD型酵素GDE4関連代謝経路のLC-MS/MSによる同定,
第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2015年10月. 松田璃沙, 坪井一人, 岡本蓉子, Rahman Ara Iffat Sonia, 山﨑尚志, 上田夏生, 田中保, 德村彰 :
消化管上皮細胞に存在する新規膜結合型リゾホスホリパーゼD,
第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2015年10月. 田中保, 伊藤葵, 木村朱里, 松岡久嗣, 藤原美奈, 喜田孝史, Afroz Sheuli, 今井博之, 德村彰 :
植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性質,
第28回植物脂質シンポジウム, 2015年9月. 清水良多, 魚住幸加, 森戸克弥, 大隅隆, 德村彰, 田中保 :
脂肪酸の鎖長伸長と短縮反応におけるペルオキシソームの役割,
日本脂質栄養学会第24回大会, 2015年8月. 渋谷菜摘, 藤川昂樹, 清蔭恵美, 樋田一徳, 田中保 :
キャベツに見出されたフィトセラミド-1-リン酸のヒト胃由来MKN74細胞に対する小胞分泌作用,
日本脂質栄養学会第24回大会, 2015年8月. 木村朱里, 伊藤葵, 喜田孝史, 松岡久嗣, 德村彰, 田中保 :
野菜に含まれるフィトセラミド-1-リン酸の分布と消化,
日本脂質栄養学会第24回大会, 2015年8月. 田中保, 藤川昂樹, 森戸克弥, 清蔭恵美, 樋田一徳, 清水太郎, 石田竜弘, 德村彰 :
リゾホスファチジン酸が誘導する小胞分泌現象の解析,
第57回日本脂質生化学会, 2015年5月. 藤川昂樹, 森戸克弥, 生駒照, 清蔭恵美, 樋田一徳, 清水太郎, 石田竜弘, 德村彰, 田中保 :
ヒト胃由来培養細胞におけるリゾホスファチジン酸誘導性小胞分泌現象の解析,
日本膜学会第37年会, 2015年5月. 生駒照, 屋宜亜耶乃, 藤川昂樹, 森戸克弥, 南利夫, 德村彰, 田中保 :
穀物におけるホスファチジン酸(PA)含量とPAの抗消化性潰瘍効果,
日本薬学会第135年会, 2015年3月. 伊藤葵, 木村朱里, 松岡久嗣, 藤原美奈, 喜田孝史, 今井博之, 德村彰, 田中保 :
グリコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性状と分布,
日本薬学会第135年会, 2015年3月. 田畑優美香, 山下量平, 伊賀永里奈, 喜田孝史, 安藤千恵, 德村彰, 田中保 :
種々のセラミド-1-リン酸の生理活性,
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 221, 2014年11月. 橋村慧, 松田璃沙, 稲垣裕司, 松井寛和, 横田美帆, 田中保, 木戸淳一, 永田俊彦, 德村彰 :
歯周病におけるリゾホスファチジン酸の役割,
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 220, 2014年11月. 松岡久嗣, 伊藤葵, 木村朱里, 藤原美奈, 喜田孝史, 今井博之, 德村彰, 田中保 :
グルコシルイノシトールホスホセラミド特異的ホスホリパーゼDの性状と分布,
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 220, 2014年11月. 魚住幸加, 森戸克弥, 大隅隆, 德村彰, 田中保 :
脂肪酸リモデリング反応におけるペルオキシソームの役割,
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 119, 2014年11月. 橋村慧, 松田璃沙, 稲垣裕司, 松井寛和, 横田美帆, 田中保, 木戸淳一, 永田俊彦, 德村彰 :
大豆由来リゾホスファチジン酸の歯周病抑制効果,
日本農芸化学会中四国支部第 40回講演会, 講演要旨集, 55, 2014年9月. 魚住幸加, 森戸克弥, 大隅隆, 德村彰, 田中保 :
裸子植物に含まれるポリメチレン中断型不飽和脂肪酸の動物細胞における必須脂肪酸への変換,
日本農芸化学会中四国支部第 40回講演会, 講演要旨集, 55, 2014年9月. 橋村慧, 松田璃沙, 稲垣裕司, 松井寛和, 横田美帆, 田中保, 木戸淳一, 永田俊彦, 德村彰 :
歯周病の進行に及ぼす口腔内リゾホスファチジン酸の抑制効果,
第56回日本脂質生化学会, 講演要旨集, 146, 2014年6月. 田中保, 生駒照, 森戸克弥, 德村彰, 南利夫 :
ホスファチジン酸(PA)の抗消化性潰瘍効果と穀類におけるPA含量,
日本農芸化学会中四国支部第 38回講演会, 講演要旨集, 24, 2014年1月. 小原真純, 坂本英次郎, 田中保, 木戸淳一, 永田俊彦, 德村彰 :
骨髄幹細胞から骨芽細胞への分化におけるリゾホスファチジン酸およびその産生酵素の役割,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 2013年10月. 畠中貴代子, 山下修司, 橋村慧, 加藤瞭典, 田中保, 中山泰介, 木下肇, 原知也, 添木武, 佐田政隆, 北川哲也, 德村彰 :
心血管病変患者血漿でのリン脂質メディエーターLC-MS/MS 分析—バイオマーカーとしての有用性—,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 2013年10月. 山本藍美, 田中優, 山川祥悟, 桑原章, 田中保, 苛原稔, 德村彰 :
ラット卵胞発育および排卵調節機構におけるリゾホスファチジン酸受容体と代謝酵素の関与,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 2013年10月. 魚住幸加, 森戸克弥, 大隅隆, 田中保, 徳村彰 :
ペルオキシソーム/ミクロソームにおける鎖長短縮/伸長反応を介した脂肪酸リモデリング,
第86回日本生化学会大会, Late-breaking abstracts (1LBA-024), 2013年9月. 森戸克弥, 大本真弓, 生駒照, 木下正文, 近藤宏樹, 瓜倉真衣, 里内清, 田中保, 徳村彰 :
リゾホスファチジン酸による胃粘膜保護のメカニズム,
第86回日本生化学会大会, プログラム号, 108, 2013年9月. 瓜倉真衣, 村上泰子, 田中保 :
キャベツの調理過程における抗潰瘍性リン脂質の産生,
第60回日本栄養改善学会, 2013年9月. 盛重純一, 瓜倉真衣, 田中保, 吉本谷博, 小池透, 里内清 :
Phos-tag Toyopearlを用いた生理活性リン酸モノエステル型脂質の分析法の開発,
第55回日本脂質生化学会, 講演要旨集, 32, 2013年6月. 田中保, 喜田孝史, 今井博之, 盛重純一, 山下量平, 松岡久嗣, 魚住幸加, 里内清, 長野稔, 徳村彰 :
アブラナ科植物に見出されたグリコシルイノシトールホスホセラミド特異的ホスホリパーゼD,
第55回日本脂質生化学会, 講演要旨集, 60, 2013年6月. 田中保 :
消化管におけるリゾホスファチジン酸産生とその生理的意義,
第55回日本脂質生化学会, シンポジウム講演要旨集, 14, 2013年6月. 喜田孝史, 盛重純一, 山下量平, 松岡久嗣, 魚住幸加, 里内清, 長野稔, 吉村好之, 田中保, 德村彰 :
キャベツ葉に見出されたフィトセラミド-1-リン酸の生合成経路,
第85回日本生化学会大会, 86, 2012年12月. 山下量平, 安藤千恵, 田畑優美香, 喜田孝史, 清水嘉文, 西迫寛隆, 川添和義, 田中保, 德村彰 :
種々のセラミド-1-リン酸分子種の細胞遊走および抗アポトーシス活性,
第85回日本生化学会大会, 86, 2012年12月. 喜田孝史, 盛重純一, 山下量平, 松岡久嗣, 魚住幸加, 里内清, 長野稔, 吉村好之, 田中保, 德村彰 :
キャベツ葉に見出されたグリコシルイノシトールホスホセラミド-ホスホリパーゼD,
第25回植物脂質シンポジウム, 86, 2012年12月. 田中保, 森戸克弥, 木下正文, 大本真弓, 近藤宏樹, 瓜倉真衣, 里内清, 德村彰 :
ホスファチジン酸によるアスピリン潰瘍抑制,
第21回日本脂質栄養学会, Vol.21, No.2, 186, 2012年9月. 田中保, 森戸克弥, 木下正文, 大本真弓, 近藤宏樹, 瓜倉真衣, 里内清, 德村彰 :
ホスファチジン酸およびリゾホスファチジン酸によるアスピリン潰瘍抑制,
第65回日本酸化ストレス学会学術集会, p93, 2012年6月. 清水嘉文, 小泉恵子, 神奈木玲児, 田中広治, 山下純, 田中保, 曹科, 鈴木元, 村手隆, 岩城壮一郎, 藤井聡, 德村彰 :
ヒト大腸がん細胞における低酸素条件下でのリゾリン脂質およびエーテル型リン脂質の増加,
第54回日本脂質生化学会, 262, 2012年6月. 森戸克弥, 木下正文, 大本真弓, 近藤宏樹, 瓜倉真衣, 里内清, 田中保, 德村彰 :
ホスファチジン酸によるアスピリン潰瘍抑制,
日本農芸化学会2012年度大会, プログラム, p83, 2012年3月. 池松夏紀, 坪井一人, 井上愛美, 清水嘉文, 宇山徹, 田中保, 上田夏生, 德村彰 :
マウス全脳におけるエタノールアミン含有脂質の分子種分析と関連酵素の基質特異性解析,
第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会, 2011年11月. 淺木千佳, 佐野真純, 田中保, 德村彰 :
循環血液中での血小板活性化因子(PAF)様酸化リン脂質の生成に及ぼす喫煙の影響,
第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会, プログラム, p170, 2011年11月. 荷川取史妃, 藤近加奈子, 福田彩, 閨真梨子, 田中保, 德村彰 :
種々の動物体液中のオートタキシンによるリゾホスホリパーゼD活性とタンパク量の比較,
第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会, プログラム, p170, 2011年11月. 閨真梨子, 池松夏紀, 田中保, 安田勝彦, 神崎秀陽, 德村彰 :
血漿リゾホスホリパーゼDによるリゾホスファチジン酸産生の妊娠に伴う変動,
第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会, プログラム, p169, 2011年11月. 安藤千恵, 喜田孝史, 山下量平, 盛重純一, 里内清, 西迫寛隆, 川添和義, 田中保, 德村彰 :
キャベツ葉中に見出されたフィトセラミド-1-リン酸の生物活性,
第50回日本薬学会日本薬剤師会日本病院薬剤師会中国四国支部学術大会, プログラム, 169, 2011年11月. 清水嘉文, 森川美幸, 木元重信, 田中保, 德村彰 :
アトピー性皮膚炎発症マウスの血液中および皮膚炎発症部で高値となるリゾホスファチジン酸の病態生理学的役割,
第84回日本生化学会大会, プログラム号, p103, 2011年9月. 田中保 :
Phos-tagを用いた活性リン脂質の質量分析, --- フォーラム:Phos-tag技術が拓く新たなリン酸化シグナル研究 ∼生物種を越えて∼ ---,
第84回日本生化学会大会, プログラム号, 40, 2011年9月. 田中保, 喜田孝史, 盛重純一, 安藤千恵, 山下量平, 里内清, 今井博之, 長野稔, 西迫寛隆, 川添和義, 德村彰 :
キャベツ葉中に見出されたフィトセラミド-1-リン酸の生合成経路と生理作用,
第84回日本生化学会大会, プログラム号, p90, 2011年9月. 田中保, 葛西彩香, 木下正文, 森戸克弥, 大本真弓, 近藤宏樹, 瓜倉真衣, 盛重純一, 里内清, 德村彰 :
種々の食材のホスファチジン酸含量と胃における消化,
日本脂質栄養学第20回大会脂質栄養学, Vol.20, No.2, 143, 2011年9月. 喜田孝史, 盛重純一, 安藤千恵, 里内清, 今井博之, 長野稔, 田中保, 德村彰 :
キャベツ葉中に見出されたフィトセラミド-1-リン酸,
第52回日本生化学会中国・四国支部例会, 講演要旨集,, p76, 2011年5月. 清水嘉文, 足立美佳, 森川美幸, 木元重信, 田中保, 德村彰 :
アトピー性皮膚炎の新規掻痒因子としてのリゾホスファチジン酸の可能性,
第53回日本脂質生化学会, 講演要旨集, p110, 2011年5月. 猪野真基, 大園翔平, 清水嘉文, 井上愛美, 田中保, 德村彰 :
生理活性リン脂質リゾホスファチジン酸のラット血漿における産生および分解に及ぼす副腎摘出の効果,
日本薬学会第131年会，プログラム, 169, 2011年3月. 盛重純一, 瓜倉真衣, 葛西彩香, 田中保, 德村彰, 小池透, 里内清 :
リン酸捕獲試薬を用いた簡易リゾホスファチジン酸分析法の食品への適用,
日本農芸化学会中四国支部第29回講演会, 2011年1月. 葛西彩香, 木下正文, 足立美佳, 盛重純一, 瓜倉真衣, 里内清, 柏田良樹, 今林潔, 高石喜久, 田中保, 德村彰 :
種々の食材中の創傷治癒性リン脂質含量,
日本農芸化学会中国四国支部第29回講演会，講演要旨集, 28, 2011年1月. 木下正文, 足立美佳, 葛西彩香, 大本真弓, 田中保, 盛重純一, 瓜倉真衣, 近藤宏樹, 里内清, 德村彰 :
メディエーターリン脂質による抗消化性潰瘍効果,
日本農芸化学会中国四国支部第29回講演会，講演要旨集, 28, 2011年1月. 盛重純一, 瓜倉真衣, 葛西彩香, 田中保, 德村彰, 小池透, 里内清 :
リン脂質捕獲試薬を用いた簡易リゾホスファチジン酸分析法の食品への適用,
日本農芸化学会中国四国支部第29回講演会，講演要旨集, 28, 2011年. 三木敏史, 清水嘉文, 井上友希子, 田中保, 德村彰 :
Mass spectrometric analysis of dicarboxylate semialdehyde-containing lysophosphatidylcholine produced during peroxidation of lysophosphatidylcholine by lipoxygenase,
第33回日本分子生物学会・第83会日本生化学会合同大会，プログラム, 317, 2010年12月. 片山貴大, 西本真梨子, 清水嘉文, 稲葉真衣子, 田中保, 德村彰 :
Exposure to the surface of cultured vascular smooth muscle cells of lysophospholipid and their extracellular release,
第33回日本分子生物学会・第83会日本生化学会合同大会，プログラム, 317, 2010年12月. 田中保, 盛重純一, 喜田孝史, 安藤千恵, 木下正文, 葛西彩香, 大本真弓, 瓜倉真衣, 里内清, 德村彰 :
キャベツ葉中に見出されたフィトセラミド-1-リン酸,
第33回日本分子生物学会・第83回日本生化学会大会合同大会，プログラム, 315, 2010年12月. 山本淳平, 山里将士, 桑原絵美, 清水嘉文, 朝治広貴, 田中保, 桑原章, 德村彰 :
不妊治療患者の卵胞液におけるオートタキシンによる生理活性リゾリン脂質の産生,
第49回日本薬学会・薬剤師会・日本病院薬剤師会中国四国支部学術大会，講演要旨集, 126, 2010年11月. Akira Tokumura, Yamamoto Junpei, YAmazato Mssashi, Nikawadori Miki, Shimizu Yoshinobu and Tamotsu Tanaka :
Preferable production of lysophosphatidic acids having a polyunsaturated fatty acyl group by lysophospholipase D activity of autotaxin in fresh follicular fluid from patients programmed with in vitro fertilization.,
Sep. 2010. 田中保, 盛重純一, 喜田孝史, 安藤千恵, 木下正文, 葛西彩香, 大本真弓, 瓜倉真衣, 里内清, 德村彰 :
キャベツ葉に見出されたフィトセラミド-1-リン酸,
第28回日本農芸化学会中四国支部講演会講演要旨集, 65, 2010年9月. 瓜倉真衣, 盛重純一, 森一弘, 横川和弘, 葛西彩香, 田中保, 德村彰, 里内清 :
抗潰瘍作用を目的とするホスホリパーゼD活性の高い野菜の有効な摂取法,
日本脂質栄養学会第19回大会，脂質栄養学, Vol.19, 194, 2010年9月. 菊田安至, 福島法夫, 古川真由美, 田中保, 里内清 :
ωヒドロキシ脂肪酸の定量分析,
第52回日本脂質生化学研究会，講演要旨集, 106, 2010年6月. 田中保, 盛重純一, 喜田孝史, 安藤千恵, 木下正文, 葛西彩香, 大本真弓, 瓜倉真衣, 里内清, 德村彰 :
キャベツ葉中に見出されたフィトセラミド-1-リン酸,
第52回日本脂質生化学会，講演要旨集, 77, 2010年6月. 片山貴大, 西本真梨子, 清水嘉文, 稲葉真衣子, 田中保, 德村彰 :
リゾリン脂質メディエーターのラット大動脈血管平滑筋細胞表面への露出と細胞外への遊離,
日本薬学会第130年会，講演要旨集3, 121, 2010年3月. 三木敏史, 清水嘉文, 井上友希子, 田中保, 德村彰 :
多価不飽和脂肪酸含有リゾホスファチジルコリンのリポキシゲナーゼ誘導過酸化反応に伴い生成する二次成績体の構造解析,
日本薬学会第130年会講演要旨集3, 121, 2010年3月. 三木敏史, 清水嘉文, 井上友希子, 田中保, 德村彰 :
多価不飽和脂肪酸含有ホスファチジルコリンのリポキシゲナーゼ誘導過酸化反応に伴い生成する二次成績体の構造解析,
日本薬学会第130年会，講演要旨集3, 121, 2010年3月. 木下正文, 近藤宏樹, 足立美佳, 瓜倉真衣, 盛重純一, 里内清, 田中保, 德村彰 :
キャベツの摂取によって胃の中で生じる創傷治癒因子としてのリゾホスファチジン酸,
日本薬学会第130年会講演要旨集3, 85, 2010年3月. 盛重純一, 田中保, 里内清, 小池透 :
リン酸捕獲試薬，Phos-tagを用いた生理活性リゾリン脂質の分析法の開発,
広島バイオテクノロジー研究成果発表会, 2010年3月. 近藤宏樹, 瓜倉真衣, 木下正文, 足立美佳, 盛重純一, 德村彰, 田中保, 里内清 :
消化管で生じるリゾホスファチジン酸の胃粘膜への作用,
日本農芸化学会中四国支部第26回講演会, 36, 2010年1月. 足立美佳, 盛重純一, 田中保, 寺尾純二, 里内清, 德村彰 :
食品素材や漢方薬に含まれるリゾホスファチジン酸のラットストレス性胃潰瘍に対する抑制作用,
第48回日本薬学会/日本薬剤師会/日本病院薬剤師会中国四国支部学術大会, 2009年11月. 猪野真基, 大園翔平, 清水嘉文, 上田香織, Tamotsu Tanaka and Akira Tokumura :
ラット血漿におけるリゾホスホリパーゼDによる生理活性リン脂質LPA産生に及ぼす副腎摘出の影響,
第48回日本薬学会/日本薬剤師会/日本病院薬剤師会中国四国支部学術大会, Nov. 2009. 足立美佳, 盛重純一, 田中保, 寺尾純二, 里内清, 德村彰 :
水浸拘束ストレス負荷によるラット胃潰瘍形成に及ぼす食物やリゾホスファチジン酸の抑制効果,
第82回日本生化学会大会, 2009年10月. 盛重純一, 瓜倉真衣, 田中保, 小池透, 里内清 :
リン酸捕獲試薬，Phos-tagを用いた生理活性リン酸モノエステル型リゾリン脂質の分析,
第82回日本生化学大会, 2009年10月. 田中保, 近藤宏樹, 木下正文, 足立美佳, 瓜倉真衣, 盛重純一, 里内清, 德村彰 :
野菜の消化で生じるリゾホスファチジン酸,
日本脂質栄養学会第18回大会, Vol.18, 180, 2009年9月. 盛重純一, 瓜倉真衣, 田中保, 小池透, 里内清 :
リン酸捕獲試薬，Phos-tagを用いた生理活性リン酸モノエステル型リゾリン脂質の分析,
第51回日本脂質生化学会, 23, 2009年7月. 片山貴大, 西本真梨子, 清水嘉文, 稲葉麻衣子, 田中保, 德村彰 :
リゾリン脂質メディエーターのラット大動脈血管平滑筋細胞表面への露出と細胞外への遊離,
日本薬学会第130年会, 3-121, 2009年3月. 三木敏史, 清水嘉文, 井上友希子, 田中保, 德村彰 :
多価不飽和脂肪酸含有リゾホスファチジルコリンのリポキシゲナーゼ誘導か参加反応に伴い生成する二次成績体の構造解析,
日本薬学会第130年会, 3-121, 2009年3月. 木下正文, 近藤宏樹, 足立美佳, 瓜倉真衣, 盛重純一, 里内清, 田中保, 德村彰 :
キャベツの摂取によって胃の中で生じる創傷治癒因子としてのリゾホスファチジン酸,
日本薬学会第130年会, 3-85, 2009年3月. 田中保, 近藤宏樹, 新宅友則, 木下正文, 盛重純一, 里内清, 德村彰 :
食品の消化で生じるリゾホスファチジン酸による消化管組織修復,
日本農芸化学会2009年度大会講演要旨集, 202, 2009年3月. 盛重純一, 高木晴子, 瓜倉真衣, 平野薫, 里内清, 田中保, 小池透 :
Phos-tagを用いたスフィンゴシン-1-リン酸の質量分析計による定量法の開発,
第31回日本分子生物学会・第81回日本生化学会大会合同大会講演要旨集, 175, 2008年12月. 德村彰, 足立美佳, 稲葉真衣子, 室田佳恵子, 田中保, 寺尾純二 :
消化器系の管腔側から作用するリゾリン脂質の生理的および病態生理的役割,
第31回日本分子生物学会・第81回日本生化学会大会合同大会講演要旨集, 175, 2008年12月. 田中保, 堀内剛, 近藤宏樹, 松岡恵, 盛重純一, 平野薫, 里内清, 德村彰, 小池透 :
キャベツの消化プロセスで生じる創傷治癒ホルモン・リゾホスファチジン酸による消化管組織修復,
日本農芸化学会2008年度中四国支部大会講演要旨集, 40, 2008年9月. 高木晴子, 瓜倉真衣, 盛重純一, 平野薫, 里内清, 田中保, 小池透 :
リン酸捕獲試薬，Phos-tagを用いたスフィンゴシンー1-リン酸の質量分析計による分析,
日本農芸化学会2008年度中国四国支部大会講演要旨集, 27, 2008年9月. 里内清, 田中保, 德村彰 :
食品からのリゾホスファチジン酸による胃粘膜保護,
脂質生化学会研究, Vol.50, p12, 2008年6月. 盛重純一, 田中保, 平野薫, 里内清 :
コノテガシワ種子に含まれるジュニペロン酸の細胞増殖抑制作用,
第49回日本生化学会中国・四国支部例会講演要旨集, 67, 2008年5月. 田中保 :
消化プロセスで生じる創傷治癒ホルモンに基づいた抗胃腸傷害食の設計,
第4回農芸化学研究企画賞受賞者中間報告会, 講演要旨集, 3, 2008年3月. 田中保, 堀内剛, 平野薫, 里内清, 徳村彰 :
Effect of lysophosphatidic acid in digested cabbage leaf on damaged tissue,
第30回日本分子生物学会年会・第80回日本生化学会大会合同大会, 講演要旨集, 731, 2007年12月. 炭山隆雄, 平野薫, 田中保, 里内清, 松崎浩明 :
線虫(C. elegans)に見出されたホスホリパーゼA1,
日本農芸化学会2007年度中国四国支部大会, 講演要旨集, 35, 2007年9月. 田中保, 堀内剛, 平野薫, 徳村彰, 里内清 :
食品に含まれる創傷治癒ホルモン・リゾホスファチジン酸,
日本脂質栄養学会第16回大会脂質栄養学, Vol.16, 164, 2007年9月. 平野薫, 吉積一真, 辻智子, 田中保, 里内清 :
ウコギ葉サポニンによるリパーゼ阻害と抗肥満作用,
第48回日本生化学会中国・四国支部例会, 講演抄録, 56, 2007年5月. 田中保, 堀内剛, 平野薫, 徳村彰, 盛重純一 :
消化プロセスで生じる創傷治癒ホルモン・リゾホスファチジン酸による消化管組織修復,
第48回日本生化学会中国・四国支部例会, 講演抄録, p51, 2007年5月.

研究会・報告書: Tamotsu Tanaka :
Study on glycosylinositolphosphoceramide-phospholipase D in plants,
Research topics on plant lipids, Konan Research Institute Invited Seminar Series on Bioscience, Jul. 2018. Tamotsu Tanaka :
Bioactive phospholipid formed during digestion of plant foodstuffs and their effectson gastrointestinal integrity,
Recent Advance in Pharmaceutical Research and Development, Nov. 2013. 植野美彦, 関陽介, 依岡隆児, 和泉唯信, 二川健, 岡久玲子, 石丸直澄, 尾崎和美, 田中秀治, 寺田賢治, 田中保, 古屋 S. 玲, 上岡麻衣子 :
令和3年度徳島大学高等教育研究センターアドミッション部門報告書,
令和3年度徳島大学高等教育研究センターアドミッション部門報告書, 2022年3月. 田中保 :
創傷治癒性メディエーターを活性成分とする抗アスピリン潰瘍食の開発,
財団法人旗影会研究助成報告書, 2012年. 田中保 :
抗消化管潰瘍機能の強化を目的とした大豆レシチンの加工に関する研究,
飯島記念食品科学振興財団平成20年度学術研究助成金, 2010年. 里内清, 村上薫, 田中保, 太田雅也, 池口陽子 :
福山大学生命工学部での線虫C. elegans研究,
財)日本産業科学研究所研究報告, Vol.11, 65-75, 2002年. 田中保 :
ポリメチレン中断型脂肪酸の代謝と生理作用,
杉山産業化学研究所年報, 85-99, 2002年. 里内清, 田中保, 平野薫 :
黒大豆よりのリパーゼ阻害タンパク質に関する研究,
平成11，12年農林水産省農林水産業特別試験研究結果概要書, 14-17, 2001年.

特許: 里内清, 田中保, 平野薫 : 生理活性リゾリン脂質の分析方法, 特願2007-504730, . 里内清, Tamotsu Tanaka and 平野薫 : Methods of analyzing physiologically active lysophospholipid, PCT Int. Appl. 2006:885730, . 吉積一真, 辻智子, 安藤英広, 田中保, 里内清 : リパーゼ阻害剤, 特願2005-171561, . 吉積一真, 辻智子, 安藤英広, 田中保, 里内清 : 新規3,4-seco-lupane型トリテルペノイドサポニン化合物, 特願2005-171553, . 田中保, 高井誠道 : セラミドの製造方法, (2021年9月), (2022年9月), 特許第05152021JP号 (2021年9月).
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 植物スフィンゴ脂質GIPCの加水分解を起点とする新奇植物免疫シグナル機構の解明 (研究課題/領域番号: 24K21848 )
特異的蛍光プローブを基盤とした抗肥満脂質代謝酵素群の統合的な理解と制御 (研究課題/領域番号: 23K10973 )
極長鎖脂肪酸の可溶化技術に基づくペルオキシソーム病の病理解明 (研究課題/領域番号: 22K06114 )
植物性食品に見出された植物型セラミド1-リン酸の消化吸収と生理作用の解析 (研究課題/領域番号: 19K05863 )
微弱電流処理による植物の形質制御システムの開発 (研究課題/領域番号: 17K19241 )
微弱電流によるナノ粒子の腫瘍内浸透・細胞取込み亢進による革新的がん治療技術の確立 (研究課題/領域番号: 17H03976 )
リン脂質誘導性ムチン小胞の分泌現象を利用した胃粘液増強食に関する研究 (研究課題/領域番号: 15K07430 )
モンゴル民族の伝統薬物調査とその有効利用に関する研究（第2次） (研究課題/領域番号: 26305003 )
植物性リン脂質の消化管粘膜維持作用に関する研究 (研究課題/領域番号: 24580185 )
新規膜結合リゾホスホリパーゼＤによるリゾホスファチジン酸産生と口腔粘膜保護 (研究課題/領域番号: 23590079 )
食品の消化に伴って生じる消化管粘膜維持因子に関する研究 (研究課題/領域番号: 21580141 )
野菜の消化プロセスで生じる創傷治癒ホルモンによる消化管創傷修復 (研究課題/領域番号: 19580155 )
野菜に含まれる創傷治癒成分・リゾホスファチジン酸による消化管組織修復 (研究課題/領域番号: 17580120 )
イノシトールリン脂質の分子種改変に伴う細胞機能変化の解析 (研究課題/領域番号: 12771422 )
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2024年12月20日更新

専門分野・研究分野: 脂質生化学 (Lipid Biochemistry)
所属学会・所属協会: 日本薬学会
日本脂質生化学会
日本農芸化学会
日本脂質栄養学会
日本生化学会
委員歴・役員歴: 日本薬学会 (学術誌編集委員 [2017年4月〜2020年3月])
日本脂質生化学会 (幹事 [2020年1月])
日本農芸化学会 (中四国支部参与 [2012年4月〜])
日本脂質栄養学会 (評議員 [2018年1月〜])
日本生化学会 (生化学編集委員 [2018年6月〜2023年5月])
日本農芸化学会 (代議員 [2016年6月〜2018年5月])
日本脂質栄養学会 (理事 [2021年1月〜])
日本生化学会 (代議員 [2021年11月])
日本生化学会 (評議員 [2020年1月])
受賞: 2000年9月, 日本脂質栄養学会ランズ奨励賞 (日本脂質栄養学会)
2007年3月, 第4回農芸化学研究企画賞 (社団法人日本農芸化学会)
活動: 徳島大学大学院社会産業理工学研究部研究推進委員会委員 (2019年4月)
徳島大学教養教育実務者連絡会議委員 (2020年4月)
自己点検・評価委員会委員 (2020年4月)
全学入学試験委員会委員 (2020年4月)
国際センター運営委員会委員 (2020年4月)
国際交流委員会委員 (2020年4月)
サマープログラム等実施委員会 (2020年4月)
国際センター協力教員 (2020年4月)

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Jグローバル最終確認日: 2024/12/21 01:23
氏名（漢字）: 田中保
氏名（フリガナ）: タナカタモツ
氏名（英字）: Tanaka Tamotsu
所属機関: 徳島大学教授

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researchmap最終確認日: 2024/12/22 01:41
氏名（漢字）: 田中保
氏名（フリガナ）: タナカタモツ
氏名（英字）: Tanaka Tamotsu
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登録日時: 2009/2/26 00:00
更新日時: 2024/12/21 06:12
アバター画像URI: https://researchmap.jp/read0181738/avatar.jpg
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所属ID: 0344000000
所属: 徳島大学
部署: 大学院社会産業理工学研究部生物資源産業学域
職名: 教授
学位: 博士(薬学)
学位授与機関: 徳島大学
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2024年12月21日更新

研究者番号: 90258301

所属（現在）: 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授

所属（過去の研究課題 情報に基づく）*注記: 2019/4/1 – 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 教授
2018/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学域), 准教授
2016/4/1 – 2017/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学系), 准教授
2015/4/1 – 2016/4/1 : 徳島大学, 大学院医歯薬学研究部, 准教授
2011/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 准教授
2013/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 准教授
2008/4/1 – 2011/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授
2007/4/1 : 福山大学, 生命工学部, 准教授
2006/4/1 : 福山大学, 生命工学部, 助教授
2000/4/1 – 2001/4/1 : 福山大学, 工学部, 講師

審査区分/研究分野

研究代表者

医学 / 薬学 / 生物系薬学
生物系 / 農学 / 農芸化学 / 食品科学
小区分38050:食品科学関連
小区分43020:構造生物化学関連

研究代表者以外

生物系 / 医歯薬学 / 薬学 / 生物系薬学
生物系 / 医歯薬学 / 薬学 / 天然資源系薬学
生物系 / 農学 / 農芸化学 / 食品科学
農芸化学およびその関連分野
生物系 / 医歯薬学 / 薬学 / 物理系薬学
小区分59040:栄養学および健康科学関連
中区分38:農芸化学およびその関連分野

キーワード

研究代表者

ホスファチジルイノシトール / リン脂質 / ポリメチレン中断型脂肪酸 / アラキドン酸 / 細胞増殖 / 2-アラキドノイルグリセロール / カルシウムイオン / シアドン酸 / 脂肪酸不飽和化酵素 / 食品機能学 / 消化管創傷修復 / 野菜 / 食品機能 / 消化管潰瘍 / 創傷治癒 / リン脂質メディエーター / リゾホスファチジン酸 / ホスホリパーゼ / 植物 / 食品 / 消化管粘膜保護 / ホスファチジン酸 / 抗潰瘍 / セラミド1-リン酸 / 消化管 / 粘膜上皮細胞 / リゾボスファチジン酸 / 抗消化性潰瘍 / 粘膜上皮組織 / 細胞遊走 / 植物性食品 / 粘液分泌 / 脂質メディエーター / 胃粘膜 / 表層粘液細胞 / ムチン / アスピリン潰瘍 / 消化管粘膜 / phospholipid / lysophosphatidic acid / phosphatidic acid / anti-ulcer / ceramide-1-phosphate / apoptosis / prostaglandin E2 / cyclooxygenase 2 / 胃潰瘍 / 胃粘膜増強 / 生薬 / 胃粘膜保護 / NSAIDs / 胃粘膜防御 / 抗胃潰瘍食 / NSAIDs潰瘍 / 小胞分泌 / フィロポディア / 細胞膜運動 / 抗胃潰瘍 / スフィンゴ脂質 / 植物脂質 / 食事性脂質 / 消化吸収 / グリコシルイノシトールセラミド / ホスホリパーゼD / セラミドリン酸 / セラミド / スフィンゴリン脂質 / セラミド 1-リン酸 / ペルオキシソーム病 / 極長鎖脂肪酸 / 脱髄 / 神経変性 / 培養髄鞘モデル

研究代表者以外

リゾホスファチジン酸 / リゾホスホリパーゼD / リゾホスファチジルコリン / 歯肉溝浸出液 / 歯肉上皮細胞 / 口腔粘膜 / 歯周病 / 骨芽細胞 / リゾリン脂質メディエーター / リゾホスホリパーゼC / 歯肉上皮 / ドライマウス / リゾリン脂質 / ホスホリパーゼ / 歯肉 / モンゴル民族 / 伝統薬物 / 薬用資源 / 国際情報交換 / 薬用植物 / モンゴル / 生体分子 / 生理活性 / 食品 / 栄養学 / 農林水産物 / Biological molecule / biological activity / Foodstuff / Nutrition / Farm products / 微弱電流 / 植物 / siRNA送達 / 微弱電流処理 / 遺伝子発現制御 / 形質制御 / ドラッグデリバリー / ナノ粒子 / がん治療 / 脂質 / 生活習慣病 / 酵素 / 肥満 / 蛍光 / 植物スフィンゴ脂質 / グリコシルイノシトールホスホセラミド / イノシトールグリカン / 植物免疫シグナル

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安心、安価なセラミド調製法の開発

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