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髙橋章

徳島大学

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リサーチマップ・
Jグローバル
KAKEN
注目研究

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2024年11月21日更新

職名: 教授
電話: 088-633-9428
電子メール: akiratak@tokushima-u.ac.jp
学歴: 1989/3: 徳島大学医学部医学科卒業
1993/3: 徳島大学大学院医学研究科博士課程単位取得退学
学位: 博士(医学) (徳島大学) (1993年11月)
職歴・経歴: 1993/0: 徳島大学医学部基礎系医員
1994/0: Post-doctral fellow,University of Alabama at Birmingham
1995/0: Research Associate, University of Pittsburgh, School of Medicine
1996/0: Research Instructor, University of Pittsburgh, School of Medicine
1997/0: 山口大学医学部第三内科学教室医員
1998/0: 大阪大学微生物病研究所細菌感染分野助手
2000/6: 徳島大学医学部特殊栄養学教室講師
2003/7: 徳島大学医学部栄養学科助教授

専門分野・研究分野: 栄養学 (Nutrition)
生理学 (Physiology)
細菌感染症学 (Bacterial Infectious Diseases)

研究者総覧で最新データを確認する

2024年11月21日更新

専門分野・研究分野: 栄養学 (Nutrition)
生理学 (Physiology)
細菌感染症学 (Bacterial Infectious Diseases)
担当経験のある授業科目: 予防安全学特別実験 (大学院)
公衆衛生学実習 (学部)
医療栄養学概論 (大学院)
医療栄養学特論 (大学院)
医療栄養学特論(Medicinal Nutrition) (大学院)
医療栄養学特論/Medicinal Nutrition (大学院)
国際食品安全学演習 (大学院)
宇宙と栄養・医学概論 (大学院)
微生物学 (学部)
微生物学実習 (学部)
栄養と感染微生物概論 (大学院)
栄養カウンセリング論 (学部)
栄養教育論1 (学部)
栄養教育論実習 (学部)
栄養英語 (学部)
環境予防学セミナー (大学院)
環境予防学実験 (大学院)
環境栄養衛生学概論 (大学院)
臨床栄養学概論 (大学院)
食品衛生学 (学部)
食品衛生学実習 (学部)
指導経験: 研究者総覧に該当データはありませんでした。

研究者総覧で最新データを確認する

2024年11月21日更新

専門分野・研究分野: 栄養学 (Nutrition)
生理学 (Physiology)
細菌感染症学 (Bacterial Infectious Diseases)

研究テーマ: 腸管細菌感染症による消化
吸収機構の変化と解析に関する研究 (イオンチャネル, 腸管細菌感染, 消化, 吸収)

著書

Takashi Uebanso, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Glycolate as a Biological Marker of B Vitamins,
Springer, 2022.

(キーワード): Glycolate / B-vitamins / Glyoxylate / Glycolate oxidase

福田朔, 馬渡一諭, 髙橋章 :
UV-LED照射によるウイルス不活化機構,
株式会社技術情報協会, 2021年3月. 宮脇克行, 粟飯原睦美, 髙橋章, 二川健 :
LEDを用いた近未来宇宙植物工場の開発,
株式会社技術情報協会, 2020年4月. 池原敏孝, 勢井宏義, 田中弘之, 上番増喬, 北岡和義, 髙橋章, 中橋睦美, 中屋豊, 藤原広明 :
人体生理学の基礎改訂第2版,
医学出版社, 2016年8月.

(キーワード): 生理学 (physiology)

岡﨑光子, 饗場直美, 髙橋章, 馬渡一諭, 風見公子, 神田あづさ, 古賀みのり, 辻雅子, 土屋ひろ子, 角田伸代, 坪田(宇津木) 恵, 寺澤洋子, 長幡友実, 三好恵子, 山内惠子, 渡邉純子 :
栄養教育論演習(第2版),
株式会社建帛社, 2015年3月. 岡﨑光子, 岡﨑光子, 髙橋章, 馬渡一諭, 風見公子, 神田あづさ, 古賀みのり, 辻雅子, 土屋ひろ子, 角田伸代, 坪田(宇津木) 恵, 寺澤洋子, 長幡友実, 三好恵子, 山内惠子, 渡邉純子 :
栄養教育論演習,
株式会社建帛社, 2012年4月. 髙橋章 :
シンプル微生物学第5版, --- 第4編細菌学各論 1.グラム陰性通性嫌気性桿菌 ---,
南江堂, 東京, 2011年4月. 粟飯原睦美, 髙橋章 :
近紫外線発光ダイオードを用いた殺菌システム,
化学工業社, 2010年9月. Mostafa Gadelmoula, Kazuaki Mawatari, Masayuki Yamato, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Microbiology book series2(2), --- UVA-LED Air Disinfection ---,
Formatex research center, 2010. 中屋豊, 宮本賢一, 坂巻路可, 乾明夫, 合田敏尚, 南久則, 川崎英二, 長田恭一, 佐藤隆一郎, 長澤孝志, 渡邊文雄, 岡達三, 桑波田雅士, 髙橋章, 竹谷豊 :
エッセンシャル基礎栄養学,
医歯薬出版株式会社, 東京, 2005年11月.

論文

Kai Ishida, Yushi Onoda, Yasuko Kadomura-Ishikawa, Miharu Nagahashi, Michiyo Yamashita, Shiho Fukushima, Toshihiko Aizawa, Shigeharu Yamauchi, Yasuo Fujikawa, Tomotake Tanaka, Takashi Uebanso, Masatake Akutagawa, Kazuaki Mawatari and Akira Takahashi :
Development of a standard evaluation method for microbial UV sensitivity using light-emitting diodes,
Heliyon, Vol.10, No.6, e27456, 2024.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.heliyon.2024.e27456
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.heliyon.2024.e27456

(DOI: 10.1016/j.heliyon.2024.e27456) Ryo Higashiyama, Yuna Kanda, Takaaki Shimohata, Kai Ishida, Shiho Fukushima, Kohei Yamazaki, Takashi Uebanso, Kazuaki Mawatari, Takashige Kashimoto and Akira Takahashi :
Characterization of Outer Membrane Vesicles Produced by Vibrio vulnificus,
The Journal of Medical Investigation : JMI, Vol.71, No.1,2, 102-120, 2024.

(徳島大学機関リポジトリ): ● Metadata: 119259
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.71.102
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.71.102

(徳島大学機関リポジトリ: 119259, DOI: 10.2152/jmi.71.102) Takashi Uebanso, Moeka Fukui, Chisato Naito, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
SLC16a6, mTORC1, and autophagy involves ketone body excretion in the intestinal cells,
Biology, Vol.12, No.12, 1467, 2023.

(要約): Ketone bodies serve several functions in the intestinal epithelium, such as stem cell maintenance, cell proliferation and differentiation, and cancer growth. Nevertheless, there is limited understanding of the mechanisms governing the regulation of intestinal ketone body concentration. In this study, we elucidated the factors responsible for ketone body production and excretion using shRNA-mediated or pharmacological inhibition of specific genes or functions in the intestinal cells. We revealed that a fasting-mimicked culture medium, which excluded glucose, pyruvate, and glutamine, augmented ketone body production and excretion in the Caco2 and HT29 colorectal cells. This effect was attenuated by glucose or glutamine supplementation. On the other hand, the inhibition of the mammalian target of rapamycin complex1 (mTORC1) recovered a fraction of the excreted ketone bodies. In addition, the pharmacological or shbeclin1-mediated inhibition of autophagy suppressed ketone body excretion. The knockdown of basigin, a transmembrane protein responsible for targeting monocarboxylate transporters (MCTs), such as MCT1 and MCT4, suppressed lactic acid and pyruvic acid excretion but increased ketone body excretion. Finally, we found that MCT7 (SLC16a6) knockdown suppressed ketone body excretion. Our findings indicate that the mTORC1-autophagy axis and MCT7 are potential targets to regulate ketone body excretion from the intestinal epithelium.
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/biology12121467
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38132294; ● Search Scopus @ Elsevier (PMID): 38132294; ● Search Scopus @ Elsevier (DOI): 10.3390/biology12121467

(DOI: 10.3390/biology12121467, PubMed: 38132294) K. Shinoda, Kazuaki Mawatari, Ngan Thi Kim Bui, H. Hirakawa, K. Awamoto, M. Wakitani, M. Wakitani, T. Shinoda and Akira Takahashi :
Development of novel far-UVC light source for high germicidal effects and low human health risk,
2023 IEEE Photonics Conference, IPC 2023 - Proceedings, 2023.

(出版サイトへのリンク): ● Publication site (DOI): 10.1109/IPC57732.2023.10360681
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85182552166

(DOI: 10.1109/IPC57732.2023.10360681, Elsevier: Scopus) Shintaro Inoue, Takahito Watanabe, Taiki Hamaguchi, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji and Taro Mito :
Combinatorial expression of ebony and tan generates body color variation from nymph through adult stages in the cricket, Gryllus bimaculatus.,
PLoS ONE, Vol.18, No.5, 2023.

(要約): Insect body colors and patterns change markedly during development in some species as they adapt to their surroundings. The contribution of melanin and sclerotin pigments, both of which are synthesized from dopamine, to cuticle tanning has been well studied. Nevertheless, little is known about how insects alter their body color patterns. To investigate this mechanism, the cricket Gryllus bimaculatus, whose body color patterns change during postembryonic development, was used as a model in this study. We focused on the ebony and tan genes, which encode enzymes that catalyze the synthesis and degradation, respectively, of the precursor of yellow sclerotin N-β-alanyl dopamine (NBAD). Expression of the G. bimaculatus (Gb) ebony and tan transcripts tended to be elevated just after hatching and the molting period. We found that dynamic alterations in the combined expression levels of Gb'ebony and Gb'tan correlated with the body color transition from the nymphal stages to the adult. The body color of Gb'ebony knockout mutants generated by CRISPR/Cas9 systemically darkened. Meanwhile, Gb'tan knockout mutants displayed a yellow color in certain areas and stages. The phenotypes of the Gb'ebony and Gb'tan mutants probably result from an over-production of melanin and yellow sclerotin NBAD, respectively. Overall, stage-specific body color patterns in the postembryonic stages of the cricket are governed by the combinatorial expression of Gb'ebony and Gb'tan. Our findings provide insights into the mechanism by which insects evolve adaptive body coloration at each developmental stage.
(キーワード): Animals / Melanins / Gryllidae / Nymph / ドーパミン (dopamine) / Insect Proteins
(徳島大学機関リポジトリ): ● Metadata: 118982
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0285934
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37200362; ● Search Scopus @ Elsevier (PMID): 37200362; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0285934

(徳島大学機関リポジトリ: 118982, DOI: 10.1371/journal.pone.0285934, PubMed: 37200362) Shinta Aizawa, Takashi Uebanso, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Effects of the loss of maternal gut microbiota before pregnancy on gut microbiota, food allergy susceptibility, and epigenetic modification on subsequent generations,
Bioscience of Microbiota, Food and Health, Vol.42, No.3, 203-212, 2023.

(要約): Maternal environments affect the health of offspring in later life. Changes in epigenetic modifications may partially explain this phenomenon. The gut microbiota is a critical environmental factor that influences epigenetic modifications of host immune cells and the development of food allergies. However, whether changes in the maternal gut microbiota affect the development of food allergies and related epigenetic modifications in subsequent generations remains unclear. Here, we investigated the effects of antibiotic treatment before pregnancy on the development of the gut microbiota, food allergies, and epigenetic modifications in F1 and F2 mice. We found that pre-conception antibiotic treatment affected the gut microbiota composition in F1 but not F2 offspring. F1 mice born to antibiotic-treated mothers had a lower proportion of butyric acid-producing bacteria and, consequently, a lower butyric acid concentration in their cecal contents. The methylation level in the DNA of intestinal lamina propria lymphocytes, food allergy susceptibility, and production of antigen-specific IgE in the F1 and F2 mice were not different between those born to control and antibiotic-treated mothers. In addition, F1 mice born to antibiotic-treated mothers showed increased fecal excretion related to the stress response in a novel environment. These results suggest that the maternal gut microbiota is effectively passed onto F1 offspring but has little effect on food allergy susceptibility or DNA methylation levels in offspring.
(出版サイトへのリンク): ● Publication site (DOI): 10.12938/bmfh.2022-093
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37404565; ● Search Scopus @ Elsevier (PMID): 37404565; ● Search Scopus @ Elsevier (DOI): 10.12938/bmfh.2022-093

(DOI: 10.12938/bmfh.2022-093, PubMed: 37404565) Kai Ishida, Takaaki Shimohata, Yuna Kanda, Quoc Anh Nguyen, Rumiko Masuda, Kohei Yamazaki, Takashi Uebanso, Kazuaki Mawatari, Takashige Kashimoto and Akira Takahashi :
Characteristic Metabolic Changes in Skeletal Muscle Due to Vibrio vulnificus Infection in a Wound Infection Model.,
mSystems, Vol.8, No.2, 2023.

(要約): V. vulnificus causes necrotizing skin and soft tissue infections (NSSTIs) in severe cases, with high mortality and sign of rapid deterioration. Despite the severity of the infection, the dysfunction of the host metabolism in skeletal muscle triggered by V. vulnificus is poorly understood. In this study, by using a mouse wound infection model, we revealed characteristic changes in muscle catabolism and energy metabolism in skeletal muscle associated with bacterial proliferation in the infected tissues. Understanding such metabolic changes in V. vulnificus-infected tissue may provide crucial information to identify the mechanism via which V. vulnificus induces severe infections. Moreover, our metabolite data may be useful for the recognition, identification, or detection of V. vulnificus infections in clinical studies.
(キーワード): Humans / Bacterial Toxins / Vibrio Infections / Virulence Factors / Muscle, Skeletal
(徳島大学機関リポジトリ): ● Metadata: 118290
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/msystems.00682-22
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36939368; ● Search Scopus @ Elsevier (PMID): 36939368; ● Search Scopus @ Elsevier (DOI): 10.1128/msystems.00682-22

(徳島大学機関リポジトリ: 118290, DOI: 10.1128/msystems.00682-22, PubMed: 36939368) Kazuaki Mawatari, Nobuya Koike, Kazunari Nohara, Marvin Wirianto, Takashi Uebanso, Takaaki Shimohata, Yasuhiro Shikishima, Hiroyuki Miura, Yoshitaka Nii, J Mark Burish, Kazuhiro Yagita, Akira Takahashi, Seung-Hee Yoo and Zheng Chen :
The Polymethoxyflavone Sudachitin Modulates the Circadian Clock and Improves Liver Physiology.,
Molecular Nutrition & Food Research, Vol.67, No.9, 2023.

(要約): This study elucidates Sudachitin as a new clock-modulating PMF with beneficial effects to improve diurnal metabolic homeostasis and liver physiology, suggesting the circadian clock as a fundamental mechanism to safeguard physiological well-being.
(キーワード): Mice / Animals / Circadian Clocks / ARNTL Transcription Factors / Flavonoids / Liver / Circadian Rhythm / CLOCK Proteins
(徳島大学機関リポジトリ): ● Metadata: 118294
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/mnfr.202200270
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36829302; ● CiNii @ 国立情報学研究所 (CRID): 1050299595815331328; ● Summary page in Scopus @ Elsevier: 2-s2.0-85150827501

(徳島大学機関リポジトリ: 118294, DOI: 10.1002/mnfr.202200270, PubMed: 36829302, CiNii: 1050299595815331328, Elsevier: Scopus) Kazuya Tanifuji, Yuji Shiozaki, Megumi Koike, Minori Uga, Mizuki Miura, Ayami Higashi, Takaaki Shimohata, Akira Takahashi, Hisayoshi Hayashi, Noriko Ishizuka, Yasuhiro Ichida, Shuichi Ohtomo, Naoshi Horiba, Ken-ichi Miyamoto and Hiroko Segawa :
Effects of EOS789, a novel pan-phosphate transporter inhibitor, on phosphate metabolism : Comparison with a conventional phosphate binder,
The Journal of Medical Investigation : JMI, Vol.70, No.1,2, 260-270, 2023.

(要約): Inorganic phosphate (Pi) binders are the only pharmacologic treatment approved for hyperphosphatemia. However, Pi binders induce the expression of intestinal Pi transporters and have limited effects on the inhibition of Pi transport. EOS789, a novel pan-Pi transporter inhibitor, reportedly has potent efficacy in treating hyperphosphatemia. We investigated the properties of EOS789 with comparison to a conventional Pi binder. Protein and mRNA expression levels of Pi transporters were measured in intestinal and kidney tissues from male Wistar rats fed diets supplemented with EOS789 or lanthanum carbonate (LC). 32Pi permeability was measured in intestinal tissues from normal rats using a chamber. Increased protein levels of NaPi-2b, an intestinal Pi transporter, and luminal Pi removal were observed in rats treated with LC but not in rats treated with EOS789. EOS789 but not LC suppressed intestinal protein levels of the Pi transporter Pit-1 and sodium/hydrogen exchanger isoform 3. 32Pi flux experiments using small intestine tissues from rats demonstrated that EOS789 may affect transcellular Pi transport in addition to paracellular Pi transport. EOS789 has differing regulatory effects on Pi metabolism compared to LC. The properties of EOS789 may compensate for the limitations of LC therapy. The combined or selective use of EOS789 and conventional Pi binders may allow tighter control of hyperphosphatemia. J. Med. Invest. 70 : 260-270, February, 2023.
(キーワード): Rats / Male / Animals / Phosphate Transport Proteins / Rats, Wistar / Hyperphosphatemia / Intestinal Absorption / Phosphates
(徳島大学機関リポジトリ): ● Metadata: 118209
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.70.260
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37164731; ● Search Scopus @ Elsevier (PMID): 37164731; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.70.260

(徳島大学機関リポジトリ: 118209, DOI: 10.2152/jmi.70.260, PubMed: 37164731) Aya Tentaku, Shusaku Kurisu, Kurumi Sejima, Toshiki Nagao, Akira Takahashi and Shigenobu Yonemura :
Proximal deposition of collagen IV by fibroblasts contributes to basement membrane formation by colon epithelial cells invitro.,
The FEBS Journal, Vol.289, No.23, 7466-7485, 2022.

(要約): The basement membrane (BM) underlying epithelial tissue is a thin layer of extracellular matrix that governs tissue integrity and function. Epithelial BMs are generally assembled using BM components secreted from two origins: epithelium and stroma. Although denovo BM formation involves self-assembly processes of large proteins, it remains unclear how stroma-derived macromolecules are transported and assembled, specifically in the BM region. In this study, we established an invitro co-culture model of BM formation in which DLD-1 human colon epithelial cells were cultured on top of collagen I gel containing human embryonic OUMS-36T-2 fibroblasts as stromal cells. A distinct feature of our system is represented by OUMS-36T-2 cells which are almost exclusively responsible for synthesis of collagen IV, a major BM component. Exploiting this advantage, we found that collagen IV incorporation was significantly impaired in culture conditions where OUMS-36T-2 cells were not allowed to directly contact DLD-1 cells. Soluble collagen IV, once diluted in the culture medium, did not accumulate in the BM region efficiently. Live imaging of fluorescently tagged collagen IV revealed that OUMS-36T-2 cells deposited collagen IV aggregates directly onto the basal surface of DLD-1 cells. Collectively, these results indicate a novel mode of collagen IV deposition in which fibroblasts proximal to epithelial cells exclusively contribute to collagen IV assembly during BM formation.
(キーワード): コラーゲン (collagen) / 細胞外マトリックス (extracellular matrix) / 共培養 / 腸管上皮 / 線維芽細胞
(徳島大学機関リポジトリ): ● Metadata: 117543
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/febs.16559
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35730982; ● Search Scopus @ Elsevier (PMID): 35730982; ● Search Scopus @ Elsevier (DOI): 10.1111/febs.16559

(徳島大学機関リポジトリ: 117543, DOI: 10.1111/febs.16559, PubMed: 35730982) Tomoki Ozaki, Yuta Yoshino, Ayumi Tachibana, Hideaki Shimizu, Takaaki Mori, Tomohiko Nakayama, Kazuaki Mawatari, Shusuke Numata, Junichi Iga, Akira Takahashi, Tetsuro Ohmori and Shu-ichi Ueno :
Metabolomic alterations in the blood plasma of older adults with mild cognitive impairment and Alzheimer's disease (from the Nakayama Study).,
Scientific Reports, Vol.12, No.1, 15205, 2022.

(要約): Alzheimer's disease (AD) is a progressive disease, and the number of AD patients is increasing every year as the population ages. One of the pathophysiological mechanisms of AD is thought to be the effect of metabolomic abnormalities. There have been several studies of metabolomic abnormalities of AD, and new biomarkers are being investigated. Metabolomic studies have been attracting attention, and the aim of this study was to identify metabolomic biomarkers associated with AD and mild cognitive impairment (MCI). Of the 927 participants in the Nakayama Study conducted in Iyo City, Ehime Prefecture, 106 were selected for this study as Control (n = 40), MCI (n = 26), and AD (n = 40) groups, matched by age and sex. Metabolomic comparisons were made across the three groups. Then, correlations between metabolites and clinical symptoms were examined. The blood mRNA levels of the ornithine metabolic enzymes were also measured. Of the plasma metabolites, significant differences were found in ornithine, uracil, and lysine. Ornithine was significantly decreased in the AD group compared to the Control and MCI groups (Control vs. AD: 97.2 vs. 77.4; P = 0.01, MCI vs. AD: 92.5 vs. 77.4; P = 0.02). Uracil and lysine were also significantly decreased in the AD group compared to the Control group (uracil, Control vs. AD: 272 vs. 235; P = 0.04, lysine, Control vs. AD: 208 vs. 176; P = 0.03). In the total sample, the MMSE score was significantly correlated with lysine, ornithine, thymine, and uracil. The Barthel index score was significantly correlated with lysine. The instrumental activities of daily living (IADL) score were significantly correlated with lysine, betaine, creatine, and thymine. In the ornithine metabolism pathway, the spermine synthase mRNA level was significantly decreased in AD. Ornithine was decreased, and mRNA expressions related to its metabolism were changed in the AD group compared to the Control and MCI groups, suggesting an association between abnormal ornithine metabolism and AD. Increased betaine and decreased methionine may also have the potential to serve as markers of higher IADL in elderly persons. Plasma metabolites may be useful for predicting the progression of AD.
(キーワード): Activities of Daily Living / Aged / Alzheimer Disease / Betaine / Biomarkers / Cognitive Dysfunction / Humans / Lysine / Ornithine / プラズマ (plasma) / RNA, Messenger / Thymine
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-022-19670-y
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36075959; ● Search Scopus @ Elsevier (PMID): 36075959; ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-022-19670-y

(DOI: 10.1038/s41598-022-19670-y, PubMed: 36075959) Toshiharu Kamishikiryo, Go Okada, Eri Itai, Yoshikazu Masuda, Satoshi Yokoyama, Masahiro Takamura, Manabu Fuchikami, Atsuo Yoshino, Kazuaki Mawatari, Shusuke Numata, Akira Takahashi, Tetsuro Ohmori and Yasumasa Okamoto :
Left DLPFC activity is associated with plasma kynurenine levels and can predict treatment response to escitalopram in major depressive disorder.,
Psychiatry and Clinical Neurosciences, Vol.76, No.8, 367-376, 2022.

(要約): Blood metabolite levels and resting-state brain activity were measured in patients with moderate to severe depression (n = 65) before and after 6-8 weeks of treatment with escitalopram, and these were compared between Responders and Nonresponders to treatment. We then examined the relationship between blood metabolites and brain activity related to treatment responsiveness in patients and healthy controls (n = 36).
(キーワード): Depressive Disorder, Major / Escitalopram / Humans / Kynurenine / 磁気共鳴映像法 (magnetic resonance imaging) / Prefrontal Cortex / Transcranial Magnetic Stimulation
(徳島大学機関リポジトリ): ● Metadata: 117849
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/pcn.13373
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35543406; ● Search Scopus @ Elsevier (PMID): 35543406; ● Search Scopus @ Elsevier (DOI): 10.1111/pcn.13373

(徳島大学機関リポジトリ: 117849, DOI: 10.1111/pcn.13373, PubMed: 35543406) Shiho Fukushima, Takaaki Shimohata, Yuri Inoue, Junko Kido, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Recruitment of LC3 by Campylobacter jejuni to Bacterial Invasion Site on Host Cells via the Rac1-Mediated Signaling Pathway,
Frontiers in Cellular and Infection Microbiology, Vol.12, 829682, 2022.

(要約): Campylobacter jejuni is a leading cause of food-borne disease worldwide. The pathogenicity of C. jejuni is closely associated with the internalization process in host epithelial cells, which is related to a host immune response. Autophagy indicates a key role in the innate immune system of the host to exclude invasive pathogens. Most bacteria are captured by autophagosomes and degraded by autophagosome-lysosome fusion in host cells. However, several pathogens, such as Salmonella and Shigella, avoid and/or escape autophagic degradation to establish infection. But autophagy involvement as a host immune response to C. jejuni infection has not been clarified. This study revealed autophagy association in C. jejuni infection. During infection, C. jejuni activated the Rho family small GTPase Rac1 signaling pathway, which modulates actin remodeling and promotes the internalization of this pathogen. In this study, we found the LC3 contribution to C. jejuni invasion signaling via the Rac1 signaling pathway. Interestingly, during C. jejuni invasion, LC3 was recruited to bacterial entry site depending on Rac1 GTPase activation just at the early step of the infection. C. jejuni infection induced LC3-II conversion, and autophagy induction facilitated C. jejuni internalization. Also, autophagy inhibition attenuated C. jejuni invasion step. Moreover, Rac1 recruited LC3 to the cellular membrane, activating the invasion of C. jejuni. Altogether, our findings provide insights into the new function of LC3 in bacterial invasion. We found the interaction between the Rho family small GTPase, Rac1, and autophagy-associated protein, LC3.
(キーワード): 細菌 (bacteria) / Campylobacter Infections / Campylobacter jejuni / Epithelial Cells / Humans / Microtubule-Associated Proteins / シグナル伝達 (signal transduction) / Virulence / rac1 GTP-Binding Protein
(徳島大学機関リポジトリ): ● Metadata: 117002
(出版サイトへのリンク): ● Publication site (DOI): 10.3389/fcimb.2022.829682
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35310852; ● Search Scopus @ Elsevier (PMID): 35310852; ● Search Scopus @ Elsevier (DOI): 10.3389/fcimb.2022.829682

(徳島大学機関リポジトリ: 117002, DOI: 10.3389/fcimb.2022.829682, PubMed: 35310852) Ngan Thi Kim Bui, Kazuaki Mawatari, Takahiro Emoto, Shiho Fukushima, Takaaki Shimohata, Takashi Uebanso, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength.,
Journal of Photochemistry and Photobiology B: Biology, Vol.228, 112410, 2022.

(要約): Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 suspensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, protein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260- and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310- and 365-nm UV-LEDs. Meanwhile, 260- and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260- and 280-nm UV-LEDs, 310- and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.
(キーワード): Disinfection / Herpesvirus 1, Human / Humans / Ultraviolet Rays / Viral Structures / Virus Inactivation / Water Purification
(徳島大学機関リポジトリ): ● Metadata: 117004
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jphotobiol.2022.112410
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35193038; ● Search Scopus @ Elsevier (PMID): 35193038; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jphotobiol.2022.112410

(徳島大学機関リポジトリ: 117004, DOI: 10.1016/j.jphotobiol.2022.112410, PubMed: 35193038) Tsubasa Kondo, Takashi Uebanso, Natsuki Arao, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.,
Journal of Nutritional Science and Vitaminology, Vol.68, No.3, 204-212, 2022.

(要約): Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively. Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis. In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice. We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment. mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon. Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice. Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
(キーワード): Animals / Colitis / Colon / Dextran Sulfate / Disease Models, Animal / Gene Deletion / 炎症 (inflammation) / Mice / Mice, Inbred C57BL / ノックアウトマウス (knockout mice) / RNA, Messenger / Taste
(出版サイトへのリンク): ● Publication site (DOI): 10.3177/jnsv.68.204
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35768251; ● CiNii @ 国立情報学研究所 (CRID): 1390011097096971008; ● Summary page in Scopus @ Elsevier: 2-s2.0-85133147656

(DOI: 10.3177/jnsv.68.204, PubMed: 35768251, CiNii: 1390011097096971008, Elsevier: Scopus) Takashi Uebanso, Mai Suyama, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Effect of Vitamin B2-Deficient Diet on Hydroxyproline- or Obesity-Induced Hyperoxaluria in Mice.,
Molecular Nutrition & Food Research, Vol.65, No.15, e2100226, 2021.

(要約): Together these results suggest that VB2 restriction could be a new dietary approach to improve hyperoxaluria when endogenous production of oxalate is increased.
(キーワード): Alcohol Oxidoreductases / Animals / Creatinine / Diet / Hydroxyproline / Hyperoxaluria / Kidney / 男性 (male) / Mice, Inbred C57BL / Mice, Obese / 肥満症 (obesity) / Oxalates / Riboflavin / Riboflavin Deficiency
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/mnfr.202100226
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34110671; ● Search Scopus @ Elsevier (PMID): 34110671; ● Search Scopus @ Elsevier (DOI): 10.1002/mnfr.202100226

(DOI: 10.1002/mnfr.202100226, PubMed: 34110671) Hitomi Iba, Takaaki Shimohata, Junko Kido, Sho Hatayama, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Vibrio parahaemolyticus induces inflammation-associated fluid accumulation via activation of the cystic fibrosis transmembrane conductance regulator.,
The Journal of Medical Investigation : JMI, Vol.68, No.1.2, 59-70, 2021.

(要約): Vibrio parahaemolyticus is a foodborne bacterium that causes acute gastroenteritis through the consumption of contaminated, raw, or undercooked seafood. Cystic fibrosis transmembrane conductance regulator (CFTR) is a well-characterized chloride channel that regulates several other ion channels and transporters to maintain water homeostasis in the gut lumen. Also, CFTR is a main target of bacterial infection-associated diarrhea. Hence, the aim of this study was to clarify the contribution of CFTR in V. parahaemolyticus-induced diarrhea in a mouse model of intestinal loop fluid accumulation, with CFTR inhibitors and a CFTR knockout model. The results indicated that CFTR plays a critical role in fluid accumulation in response to V. parahaemolyticus infection. We also investigated the inflammatory association in CFTR-mediated V. parahaemolyticus-induced fluid secretion with cyclooxygenase inhibitors and found that fluid accumulation was decreased by inhibition of cyclooxygenase 2 produced by neutrophils. These findings suggest that V. parahaemolyticus-inducing infiltration and activation of neutrophils also participated in CFTR mediated fluid secretion. This study reveals an important relationship between V. parahaemolyticus-induced diarrhea and inflammation in a mouse model. J. Med. Invest. 68 : 59-70, February, 2021.
(キーワード): Animals / Cystic Fibrosis Transmembrane Conductance Regulator / Diarrhea / Gastroenteritis / 炎症 (inflammation) / Mice / Vibrio parahaemolyticus
(徳島大学機関リポジトリ): ● Metadata: 115628
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.68.59
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33994481; ● Search Scopus @ Elsevier (PMID): 33994481; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.68.59

(徳島大学機関リポジトリ: 115628, DOI: 10.2152/jmi.68.59, PubMed: 33994481) Kensuke Horiyama, Takahiro Emoto, Takeyuki Haraguchi, Takashi Uebanso, Yuki Naito, Takuma Gyobu, Kenta Kanemoto, Junichi Inobe, Ayumi Sano, Masatake Akutagawa and Akira Takahashi :
Bowel sound-based features to investigate the effect of coffee and soda on gastrointestinal motility,
Biomedical Signal Processing and Control, Vol.66, 102425, 2021.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bspc.2021.102425
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85101785195

(DOI: 10.1016/j.bspc.2021.102425, Elsevier: Scopus) Miki Yasui-Maetani, Kazuaki Mawatari, Airi Honjo, Bui Kim Thi Ngan, Takaaki Shimohata, Takashi Uebanso, Mutsumi Aihara, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu,
Applied Sciences, Vol.10, No.16, 2020.

(徳島大学機関リポジトリ): ● Metadata: 115319
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/app10165549
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.3390/app10165549

(徳島大学機関リポジトリ: 115319, DOI: 10.3390/app10165549) Toshiya Okahisa, Masahiro Sogabe, Tadahiko Nakagawa, Kumiko Tanaka, Tetsu Tomonari, Tatsuya Taniguchi, Akira Takahashi, Yohsuke Kinouchi, Junji Nishioka, Naoki Igata, Hiroaki Yanagawa, takatoshi Komatsu, Yoshiaki Ohnishi, Masashi Fukuhara, Masashi Ishikawa, Hiroshi Shibata, Hirohiko Shinomiya, Masahiko Nakasono, Fumiko Kishi, Keiko Komai, Yayoi Tatsuki, Toru Murashima, Yoshihiro Deguchi, Hiroshi Aramaki, Hideyuki Fukumitsu and Tetsuji Takayama :
Development of a novel automatic ascites filtration and concentration equipment with multi-ring-type roller pump units for cell-free and concentrated ascites reinfusion therapy.,
Artificial Organs, Vol.44, No.8, 856-872, 2020.

(要約): Cell-free and concentrated ascites reinfusion therapy (CART) is an effective therapy for refractory ascites. However, CART is difficult to perform as ascites filtration and concentration is a complicated procedure. Moreover, the procedure requires the constant assistance of a clinical engineer or/and the use of an expensive equipment for the multi-purpose blood processing. Therefore, we developed a CART specialized equipment (mobility CART [M-CART]) that could be used safely with various safety measures and automatic functions such as automatic washing of clogged filtration filter and self-regulation of the concentration ratio. Downsizing, lightning of the weight, and automatic processing in M-CART required the use of newly developed multi-ring-type roller pump units. This equipment was approved under Japanese regulations in 2018. In performing 41 sessions of CART (for malignant ascites, 22 sessions; and hepatic ascites, 19 sessions) using this equipment in 17 patients, no serious adverse event occurred. An average of 4494 g of ascites was collected and the total amount of ascites was processed in all the sessions without any trouble. The mean weight of the processed ascites was 560 g and the mean concentration ratio was 8.0. The ascites were processed at a flow rate of 50 mL/min. The mean ascites processing time was 112.5 minutes and a 106.5-minutes (95.2%) ascites processing was performed automatically. The operator responded to alarms or support information 3.2 times on average (3.1 minutes, 2.1% of ascites processing time). Human errors related to ascites processing were detected by M-CART at 0.4 times per session on average and were appropriately addressed by the operator. The frequencies of automatic washing of clogged filtration filter and self-regulation of the concentration ratio were 31.7% and 53.7%, respectively. The mean recovery rates (recovery dose) of protein, albumin, and immunoglobulin G were 72.9%, 72.9%, and 71.2% (65.9 g, 34.9 g, and 13.2 g), respectively. Steroids were administered in 92.7% of the sessions to prevent fever and the mean increase in body temperature was 0.53°C. M-CART is a compact and lightweight automatic CART specialized equipment that can safely and easily process a large quantity of ascites without the constant assistance of an operator.
(徳島大学機関リポジトリ): ● Metadata: 115878
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/aor.13681
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32187379; ● Search Scopus @ Elsevier (PMID): 32187379; ● Search Scopus @ Elsevier (DOI): 10.1111/aor.13681

(徳島大学機関リポジトリ: 115878, DOI: 10.1111/aor.13681, PubMed: 32187379) Quoc Anh Nguyen, Takaaki Shimohata, Sho Hatayama, Aya Tentaku, Junko Kido, Huong Thi Mai Bui, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Central Carbon Metabolism in Epithelial Cells.,
mSphere, Vol.5, No.2, e00960--19, 2020.

(要約): infection.
(徳島大学機関リポジトリ): ● Metadata: 114634
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/mSphere.00960-19
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32188755; ● Search Scopus @ Elsevier (PMID): 32188755; ● Search Scopus @ Elsevier (DOI): 10.1128/mSphere.00960-19

(徳島大学機関リポジトリ: 114634, DOI: 10.1128/mSphere.00960-19, PubMed: 32188755) Takashi Uebanso, Ayumi Yoshimoto, Shinta Aizawa, Maya Nakamura, Rumiko Masuda, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Glycolate is a Novel Marker of Vitamin B2 Deficiency Involved in Gut Microbe Metabolism in Mice.,
Nutrients, Vol.12, No.3, E736, 2020.

(要約): (VB2) deficiency, and show that gut microbiota sense dietary VB2 deficiency and accumulate GA in response. The plasma GA concentration responded to reduced VB2 supply from both the gut microbiota and the diet. These results suggest that GA is a novel marker that can be used to assess whether or not the net supply of VB2 from dietary sources and gut microbiota is sufficient. We also found that gut microbiota can provide short-term compensation for host VB2 deficiency when dietary VB2 is withheld.
(徳島大学機関リポジトリ): ● Metadata: 115656
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/nu12030736
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32168816; ● Search Scopus @ Elsevier (PMID): 32168816; ● Search Scopus @ Elsevier (DOI): 10.3390/nu12030736

(徳島大学機関リポジトリ: 115656, DOI: 10.3390/nu12030736, PubMed: 32168816) Maria Ulfa, Momoyo Azuma, Masami Satou, Takaaki Shimohata, Shiho Fukushima, Junko Kido, Mariko Nakamoto, Takashi Uebanso, Kazuaki Mawatari, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Inactivation of Extended-spectrum β-Lactamase (ESBL)-producing Escherichia Coli by UVA-LED Irradiation System.,
The Journal of Medical Investigation : JMI, Vol.67, No.1-2, 163-169, 2020.

(要約): The prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is increasing rapidly and spreading worldwide, particularly in Asia, compared to other regions. In the last ten years, in our hospital, in particular, there has been a < 30% increase. To prevent the spread of ESBL in hospitals and the community, the ultraviolet (UV) A-light-emitting diode (LED) irradiation device was used to inactivate ESBL-E. coli in human livestock and the environment. ESBL-E. coli and E. coli bacterial samples were collected from patients at Tokushima University Hospital (Tokushima City, Japan). The UVA-LED irradiation system had 365 nm single wavelength, and the current of the circuit was set to 0.23 or 0.50 A consistently. Results demonstrated that UVA-LED was useful for the inactivation of ESBL-E. coli and E. coli. The minimum energy dosage required to inactivate ESBL-E. coli and E. coli was 40.76 J/cm2 (45 min) in the first type of UVA-LED and 38.85 J/cm2 (5 min) in the second type. There were no significant differences between ESBL-E. coli and E. coli. The inactivation of ESBL-E. coli was dependent on energy. These findings suggest that UVA-LED with 365 nm single wavelength could be useful for surface decontamination in healthcare facilities. J. Med. Invest. 67 : 163-169, February, 2020.
(徳島大学機関リポジトリ): ● Metadata: 114642
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.67.163
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32378601; ● Summary page in Scopus @ Elsevier: 2-s2.0-85084329310

(徳島大学機関リポジトリ: 114642, DOI: 10.2152/jmi.67.163, PubMed: 32378601, Elsevier: Scopus) Hiromichi Yumoto, Takashi Uebanso, Takaaki Shimohata and Akira Takahashi :
Current understanding of the gut microflora in subjects with nutrition-associated metabolic disorder such as obesity and/or diabetes: Is there any relevance with oral microflora?,
Current Oral Health Reports, Vol.6, No.2, 100-109, 2019.

(徳島大学機関リポジトリ): ● Metadata: 113630
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s40496-019-0221-7
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050566774698498304; ● Search Scopus @ Elsevier (DOI): 10.1007/s40496-019-0221-7

(徳島大学機関リポジトリ: 113630, DOI: 10.1007/s40496-019-0221-7, CiNii: 1050566774698498304) Takaaki Shimohata, Kazuaki Mawatari, Takashi Uebanso, Airi Honjo, Akari Tsunedomi, Sho Hatayama, Yuri Sato, Junko Kido, Risa Nishisaka, Ayumi Yoshimoto, Tomoko Yamashita, Sachie Amano, Miki Maetani-Yasui, Hitomi Iba, Yumi Harada, Mutsumi Nakahashi, Sonoko Yasui-Yamada, Yasuhiro Hamada, Tadahiko Nakagawa, Masahiro Sogabe, Takahiro Emoto, Masatake Akutagawa, Toshiya Okahisa, Yohsuke Kinouchi and Akira Takahashi :
Bacterial Contamination of Hemodialysis Devices in Hospital Dialysis Wards.,
The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 148-152, 2019.

(要約): Chronic care patients undergoing hemodialysis for treatment of end-stage renal failure experience higher rates of bloodstream-associated infection due to the patients' compromised immune system and management of the bloodstream through catheters. Staphylococcus species are acommon cause of hemodialysis catheterrelated bloodstream infections. We investigated environmental bacterial contamination of dialysis wards and contamination of hemodialysis devices to determine the source of bacteria for these infections. All bacterial samples were collected by the swab method and the agarose stamp method. And which bacterium were identified by BBL CRYSTAL Kit or 16s rRNA sequences. In our data, bacterial cell number of hemodialysis device was lower than environment of patient surrounds. But Staphylococcus spp. were found predominantly on the hemodialysis device (46.8%), especially on areas frequently touched by healthcare-workers (such as Touch screen). Among Staphylococcus spp., Staphylococcus epidermidis was most frequently observed (42.1% of Staphylococcus spp.), and more surprising, 48.2% of the Staphylococcus spp. indicated high resistance for methicillin. Our finding suggests that hemodialysis device highly contaminated with bloodstream infection associated bacteria. This study can be used as a source to assess the risk of contamination-related infection and to develop the cleaning system for the better prevention for bloodstream infections in patients with hemodialysis. J. Med. Invest. 66 : 148-152, February, 2019.
(徳島大学機関リポジトリ): ● Metadata: 113411
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.66.148
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064928; ● Search Scopus @ Elsevier (PMID): 31064928; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.66.148

(徳島大学機関リポジトリ: 113411, DOI: 10.2152/jmi.66.148, PubMed: 31064928) Aya Tentaku, Takaaki Shimohata, Sho Hatayama, Junko Kido, Quoc Anh Nguyen, Yuna Kanda, Shiho Fukushima, Takashi Uebanso, Taketoshi Iwata, Kazuaki Mawatari, Nagakatsu Harada and Akira Takahashi :
Host cellular unfolded protein response signaling regulates Campylobacter jejuni invasion.,
PLoS ONE, Vol.13, No.10, 2018.

(要約): Campylobacter jejuni is a major cause of bacterial foodborne illness in humans worldwide. Bacterial entry into a host eukaryotic cell involves the initial steps of adherence and invasion, which generally activate several cell-signaling pathways that induce the activation of innate defense systems, which leads to the release of proinflammatory cytokines and induction of apoptosis. Recent studies have reported that the unfolded protein response (UPR), a system to clear unfolded proteins from the endoplasmic reticulum (ER), also participates in the activation of cellular defense mechanisms in response to bacterial infection. However, no study has yet investigated the role of UPR in C. jejuni infection. Hence, the aim of this study was to deduce the role of UPR signaling via induction of ER stress in the process of C. jejuni infection. The results suggest that C. jejuni infection suppresses global protein translation. Also, 12 h of C. jejuni infection induced activation of the eIF2α pathway and expression of the transcription factor CHOP. Interestingly, bacterial invasion was facilitated by knockdown of UPR-associated signaling factors and treatment with the ER stress inducers, thapsigargin and tunicamycin, decreased the invasive ability of C. jejuni. An investigation into the mechanism of UPR-mediated inhibition of C. jejuni invasion showed that UPR signaling did not affect bacterial adhesion to or survival in the host cells. Further, Salmonella Enteritidis or FITC-dextran intake were not regulated by UPR signaling. These results indicated that the effect of UPR on intracellular intake was specifically found in C. jejuni infection. These findings are the first to describe the role of UPR in C. jejuni infection and revealed the participation of a new signaling pathway in C. jejuni invasion. UPR signaling is involved in defense against the early step of C. jejuni invasion and thus presents a potential therapeutic target for the treatment of C. jejuni infection.
(キーワード): Caco-2 Cells / Campylobacter jejuni / Endoplasmic Reticulum Stress / Thapsigargin / Transcription Factor CHOP / Tunicamycin / Unfolded Protein Response
(徳島大学機関リポジトリ): ● Metadata: 114338
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0205865
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30321237; ● Search Scopus @ Elsevier (PMID): 30321237; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0205865

(徳島大学機関リポジトリ: 114338, DOI: 10.1371/journal.pone.0205865, PubMed: 30321237) Sho Hatayama, Takaaki Shimohata, Sachie Amano, Junko Kido, Q Anh Nguyen, Yuri Sato, Yuna Kanda, Aya Tentaku, Shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Invasion and Inflammatory Barrier Disruption Promoting Bacterial Invasion from Lateral Membrane in Polarized Intestinal Epithelial Cells.,
Frontiers in Cellular and Infection Microbiology, Vol.8, No.30, 2018.

(要約): Campylobacter jejuni invasion is closely related to C. jejuni pathogenicity. The intestinal epithelium contains polarized epithelial cells that form tight junctions (TJs) to provide a physical barrier against bacterial invasion. Previous studies indicated that C. jejuni invasion of non-polarized cells involves several cellular features, including lipid rafts. However, the dynamics of C. jejuni invasion of polarized epithelial cells are not fully understood. Here we investigated the interaction between C. jejuni invasion and TJ formation to characterize the mechanism of C. jejuni invasion in polarized epithelial cells. In contrast to non-polarized epithelial cells, C. jejuni invasion was not affected by depletion of lipid rafts in polarized epithelial cells. However, depletion of lipid rafts significantly decreased C. jejuni invasion in TJ disrupted cells or basolateral infection and repair of cellular TJs suppressed lipid raft-mediated C. jejuni invasion in polarized epithelial cells. In addition, pro-inflammatory cytokine, TNF- treatment that induce TJ disruption promote C. jejuni invasion and lipid rafts depletion significantly reduced C. jejuni invasion in TNF- treated cells. These data demonstrated that TJs prevent C. jejuni invasion from the lateral side of epithelial cells, where they play a main part in bacterial invasion and suggest that C. jejuni invasion could be increased in inflammatory condition. Therefore, maintenance of TJs integrity should be considered important in the development of novel therapies for C. jejuni infection.
(徳島大学機関リポジトリ): ● Metadata: 111607
(出版サイトへのリンク): ● Publication site (DOI): 10.3389/fcimb.2018.00015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29441328; ● Search Scopus @ Elsevier (PMID): 29441328; ● Search Scopus @ Elsevier (DOI): 10.3389/fcimb.2018.00015

(徳島大学機関リポジトリ: 111607, DOI: 10.3389/fcimb.2018.00015, PubMed: 29441328) Ayumi Yoshimoto, Takashi Uebanso, Mutsumi Nakahashi, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Effect of prenatal administration of low dose antibiotics on gut microbiota and body fat composition of newborn mice.,
Journal of Clinical Biochemistry and Nutrition, Vol.62, No.2, 155-160, 2017.

(要約): Several environmental factors during the prenatal period transgenerationally affect the health of newborns in later life. Because low-dose antibiotics have been used for promoting the growth of crops and livestock in agriculture, humans may have ingested residual antibiotics for several decades. However, the effect of prenatal administration of low-dose antibiotics on newborns' health in later life is unclear. In the present study, we found that prenatal treatment of murine mothers with low-dose antibiotics increased the abundance of bacteria of the phylum Firmicutes and the genera Clostridium IV and XIVa in feces from pups. In addition, the body fat percentage of mice in the antibiotic-treated group was higher than those in the control group at 12 weeks of age even though all pups were fed a standard diet. The body fat percentage of all mice was correlated with the abundance of fecal bacteria of Clostridium IV and XIVa. These results predict that low-dose antibiotic administration during the prenatal period affects the gut microbiota of newborns and possibly their health in later life.
(徳島大学機関リポジトリ): ● Metadata: 114647
(出版サイトへのリンク): ● Publication site (DOI): 10.3164/jcbn.17-53
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29610555; ● Search Scopus @ Elsevier (PMID): 29610555; ● Search Scopus @ Elsevier (DOI): 10.3164/jcbn.17-53

(徳島大学機関リポジトリ: 114647, DOI: 10.3164/jcbn.17-53, PubMed: 29610555) Junko Kido, Takaaki Shimohata, Sachie Amano, Syo Hatayama, Quoc Anh Nguyen, Yuri Sato, Yuna Kanda, Aya Tentaku, Shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
CFTR reduces microtubule-dependent Campylobacter jejuni invasion.,
Infection and Immunity, 2017.

(要約): Campylobacter jejuni (C. jejuni) is gastroenteritis inducible food-born pathogen. Invasion and adhesion process are essential for leading gastroenteritis in C. jejuni infection process. As against bacterial strategy for efficacy invasion and adhesion, mucosal layer play a key role in defense systems, which modulated by several ion channels and transporters mediated water flux on the intestine. Cystic fibrosis transmembrane conductance regulator (CFTR) play the main role in waterfulux in intestine, and it closely related with bacterial clearance. We previously reported that C. jejuni infection suppresses CFTR channel activity in intestinal epithelial cells, however the mechanism and importance of this suppression is unclear. This study seeks to elucidate the role of CFTR in C. jejuni-infection. Using HEK293 cells that stably express wild type and mutated CFTR, we found that CFTR attenuated C. jejuni invasion, it was not involved bacterial adhesion or intracellular survival but associated with microtubule-dependent cellular transport. Moreover we revealed that CFTR attenuated function of microtubule motor protein but not microtubule stability, which causes inhibition of C. jejuni-invasion. Meanwhile, the CFTR mutant G551D-CFTR, which has defects in channel activity, suppressed C. jejuni-invasion, whereasF508-CFTR, which has defects in maturation, did not suppress, suggesting that CFTR suppression of C. jejuni-invasion is related to CFTR maturation but not channel activity.Taken together, mature CFTR inhibited C. jejuni invasion by regulating microtubule-mediated pathways. We suggest that CFTR plays a critical role in cellular defenses against C. jejuni-invasion, and CFTR suppression may be an initial step in promoting cellular invasion during C. jejuni-infection.
(徳島大学機関リポジトリ): ● Metadata: 114676
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.00311-17
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28784926; ● Search Scopus @ Elsevier (PMID): 28784926; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.00311-17

(徳島大学機関リポジトリ: 114676, DOI: 10.1128/IAI.00311-17, PubMed: 28784926) Takashi Uebanso, Saki Kano, Ayumi Yoshimoto, Chisato Naito, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Effects of Consuming Xylitol on Gut Microbiota and Lipid Metabolism in Mice.,
Nutrients, Vol.9, No.7, 756, 2017.

(要約): The sugar alcohol xylitol inhibits the growth of some bacterial species including Streptococcus mutans. It is used as a food additive to prevent caries. We previously showed that 1.5-4.0 g/kg body weight/day xylitol as part of a high-fat diet (HFD) improved lipid metabolism in rats. However, the effects of lower daily doses of dietary xylitol on gut microbiota and lipid metabolism are unclear. We examined the effect of 40 and 200 mg/kg body weight/day xylitol intake on gut microbiota and lipid metabolism in mice. Bacterial compositions were characterized by denaturing gradient gel electrophoresis and targeted real-time PCR. Luminal metabolites were determined by capillary electrophoresis electrospray ionization time-of-flight mass spectrometry. Plasma lipid parameters and glucose tolerance were examined. Dietary supplementation with low- or medium-dose xylitol (40 or 194 mg/kg body weight/day, respectively) significantly altered the fecal microbiota composition in mice. Relative to mice not fed xylitol, the addition of medium-dose xylitol to a regular and HFD in experimental mice reduced the abundance of fecal Bacteroidetes phylum and the genus Barnesiella, whereas the abundance of Firmicutes phylum and the genus Prevotella was increased in mice fed an HFD with medium-dose dietary xylitol. Body composition, hepatic and serum lipid parameters, oral glucose tolerance, and luminal metabolites were unaffected by xylitol consumption. In mice, 40 and 194 mg/kg body weight/day xylitol in the diet induced gradual changes in gut microbiota but not in lipid metabolism.
(徳島大学機関リポジトリ): ● Metadata: 115660
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/nu9070756
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28708089; ● Summary page in Scopus @ Elsevier: 2-s2.0-85025080133

(徳島大学機関リポジトリ: 115660, DOI: 10.3390/nu9070756, PubMed: 28708089, Elsevier: Scopus) Takashi Uebanso, Ai Ohnishi, Reiko Kitayama, Ayumi Yoshimoto, Mutsumi Nakahashi, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Effects of Low-Dose Non-Caloric Sweetener Consumption on Gut Microbiota in Mice.,
Nutrients, Vol.9, No.6, 2017.

(要約): Abstract: Non-caloric artificial sweeteners (NASs) provide sweet tastes to food without adding calories or glucose. NASs can be used as alternative sweeteners for controlling blood glucose levels and weight gain. Although the consumption of NASs has increased over the past decade in Japan and other countries, whether these sweeteners affect the composition of the gut microbiome is unclear. In the present study, we examined the effects of sucralose or acesulfame-K ingestion (at most the maximum acceptable daily intake (ADI) levels, 15 mg/kg body weight) on the gut microbiome in mice. Consumption of sucralose, but not acesulfame-K, for 8 weeks reduced the relative amount of Clostridiumcluster XIVa in feces. Meanwhile, sucralose and acesulfame-K did not increase food intake, body weight gain or liver weight, or fat in the epididymis or cecum. Only sucralose intake increased the concentration of hepatic cholesterol and cholic acid. Moreover, the relative concentration of butyrate and the ratio of secondary/primary bile acids in luminal metabolites increased with sucralose consumption in a dose-dependent manner. These results suggest that daily intake of maximum ADI levels of sucralose, but not acesulfame-K, affected the relative amount of the Clostridium cluster XIVa in fecal microbiome and cholesterol bile acid metabolism in mice.
(徳島大学機関リポジトリ): ● Metadata: 115661
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/nu9060560
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28587159; ● Search Scopus @ Elsevier (PMID): 28587159; ● Search Scopus @ Elsevier (DOI): 10.3390/nu9060560

(徳島大学機関リポジトリ: 115661, DOI: 10.3390/nu9060560, PubMed: 28587159) Yanfei Hou, Mutsumi Nakahashi, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Yumi Harada, Akari Tsunedomi, Takahiro Emoto, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Combined treatment of UVA irradiation and antibiotics induces greater bactericidal effects on Vibrio parahaemolyticus.,
The Journal of Medical Investigation : JMI, Vol.63, No.1-2, 63-67, 2016.

(要約): The presence of antibiotics in the environment and their subsequent impact on the development of multi-antibiotic resistant bacteria has raised concerns globally. Consequently, much research is focused on a method to produce a better disinfectant. We have established a disinfectant system using UVA-LED that inactivates pathogenic bacteria. We assessed the bactericidal efficiency of a combination of UVA-LED and antibiotics against Vibrio parahaemolyticus. Combined use of antibiotic drugs and UVA irradiation was more bactericidal than UVA irradiation or antibacterial drugs alone. The bactericidal synergy was observed at low concentrations of each drug that are normally unable to kill the bacteria. This combination has the potential to become a sterilization technology. J. Med. Invest. 63: 63-67, February, 2016.
(徳島大学機関リポジトリ): ● Metadata: 111155
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.63.63
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27040055; ● Search Scopus @ Elsevier (PMID): 27040055; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.63.63

(徳島大学機関リポジトリ: 111155, DOI: 10.2152/jmi.63.63, PubMed: 27040055) Quang Ngoc Phan, Takashi Uebanso, Takaaki Shimohata, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi :
DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus.,
Journal of Bacteriology, 2015.

(要約): Nucleoid binding protein HU regulate cellular behaviors, including nucleoid structuring, general recombination, transposition, growth, replication, motility, metabolism, and virulence. It is concerned that both number of bacteria and number of virulence factors may affect pathogenecity of bacteria. In the present study, we investigated that which factor have dominant role during infection in one of the most rapidly growing bacteria Vibrio parahaemolyticus. We found that V. parahaemolyticus cytotoxicity is regulated, in a growth rate-independent manner, by the HU proteins through regulation of number of virulence factors including T3SS1 gene expression.
(徳島大学機関リポジトリ): ● Metadata: 109503
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/JB.00306-15
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26148713; ● Search Scopus @ Elsevier (PMID): 26148713; ● Search Scopus @ Elsevier (DOI): 10.1128/JB.00306-15

(徳島大学機関リポジトリ: 109503, DOI: 10.1128/JB.00306-15, PubMed: 26148713) Toshitaka Ikehara, Mutsumi Nakahashi, Zehong Su, Masatake Akutagawa, Koichiro Tsuchiya, Mitsuo Kitamura, Akira Takahashi and Yohsuke Kinouchi :
Effects of UV-A LED light irradiation on growth of cultured RAW 264.7 cells,
Toxicological and Environmental Chemistry, Vol.97, No.2, 243-255, 2015.

(キーワード): 365 nm / LED light / 活性酸素 (reactive oxygen species) / Raw 264.7 cells / scavenger
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/02772248.2015.1039771
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84931571408

(DOI: 10.1080/02772248.2015.1039771, Elsevier: Scopus) Y Kadomura-Ishikawa, Katsuyuki Miyawaki, Akira Takahashi, Toshiya Masuda and Sumihare Noji :
Light and abscisic acid independently regulated FaMYB10 in Fragaria x ananassa fruit,
Planta, Vol.241, No.4, 953-965, 2015.

(要約): Light and ABA independently regulated anthocyanin biosynthesis via activation of FaMYB10 expression. FaMYB10 accelerated anthocyanin synthesis of pelargonidin 3-glucoside and cyanidin 3-glucoside during strawberry fruit ripening. Light is an integral factor in fruit ripening. Ripening in non-climacteric fruit is also effected by the plant hormone abscisic acid (ABA). However, how light and/or ABA regulate fruit ripening processes, such as strawberry color development remains elusive. Results of the present study showed light and ABA regulated strawberry fruit coloration via activation of FaMYB10 expression, an R2R3 MYB transcription factor. Light exposure increased FaMYB10 transcript levels, flavonoid pathway genes, and anthocyanin content. Exogenous ABA promoted FaMYB10 expression, and anthocyanin content, accompanied by increased ABA-responsive transcript levels and flavonoid pathway genes. ABA biosynthesis inhibitor treatment, and RNAi-mediated down-regulation of the ABA biosynthetic gene (9-cis epoxycarotenoid dioxygenase: FaNCED1), and ABA receptor (magnesium chelatase H subunit: FaCHLH/ABAR) showed inverse ABA effects. Furthermore, additive effects were observed in anthocyanin accumulation under combined light and ABA, indicating independent light and ABA signaling pathways. FaMYB10 down-regulation by Agrobacterium-mediated RNA interference (RNAi) in strawberry fruits showed decreased pelargonidin 3-glucoside and cyanidin 3-glucoside levels, accompanied by consistent flavonoid pathway gene expression levels. FaMYB10 over-expression showed opposite FaMYB10 RNAi phenotypes, particularly cyanidin 3-glucoside synthesis by FaMYB10, which was correlated with FaF3'H transcript levels. These data provided evidence that light and ABA promoted FaMYB10 expression, resulting in anthocyanin accumulation via acceleration of flavonoid pathway gene expression. Finally, our results suggested FaMYB10 serves a role as a signal transduction mediator from light and ABA perception to anthocyanin synthesis in strawberry fruit.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00425-014-2228-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25534946; ● Summary page in Scopus @ Elsevier: 2-s2.0-84925538505

(DOI: 10.1007/s00425-014-2228-6, PubMed: 25534946, Elsevier: Scopus) Sachie Negoro, Takaaki Shimohata, Syo Hatayama, Yuri Sato, Mari Matsumoto, Hitomi Iba, Mutsumi Aihara, Takashi Uebanso, Yasuhiro Hamada, Yoshikazu Nishikawa, Shinji Yamasaki, Kazuaki Mawatari and Akira Takahashi :
Campylobacter jejuni infection suppressed Cl(-) secretion induced by CFTR activation in T-84 cells.,
Journal of Infection and Chemotherapy, Vol.20, No.11, 682-688, 2014.

(要約): Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl(-)) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl(-) channel transporting Cl(-) to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied. Here, we used an (125)I(-) efflux assay and measurement of short-circuit current to measure Cl(-) secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl(-) secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl(-) secretion suppressed in C. jejuni-infected T-84 cells. The suppression of activated Cl(-) secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract.
(徳島大学機関リポジトリ): ● Metadata: 110108
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jiac.2014.07.007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25107576; ● Summary page in Scopus @ Elsevier: 2-s2.0-84908232552

(徳島大学機関リポジトリ: 110108, DOI: 10.1016/j.jiac.2014.07.007, PubMed: 25107576, Elsevier: Scopus) Mutsumi Nakahashi, Kazuaki Mawatari, Akiko Hirata, Miki Maetani, Takaaki Shimohata, Takashi Uebanso, Yasuhiro Hamada, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus.,
Photochemistry and Photobiology, Vol.90, No.6, 1397-1403, 2014.

(要約): Ultraviolet (UV) irradiation is an increasingly used method of water disinfection. UV rays can be classified by wavelength into UVA (320-400 nm), UVB (280-320 nm), and UVC (< 280 nm). We previously developed UVA sterilization equipment with a UVA-light emitting diode (LED). The aim of this study was to establish a new water disinfection procedure using the combined irradiation of the UVA-LED and another UV wavelength. An oxidative DNA product, 8-hydroxy-2'-deoxyguanosine (8-OHdG), increased after irradiation by UVA-LED alone, and the level of cyclobutane pyrimidine dimers (CPDs) was increased by UVC alone in Vibrio parahaemolyticus. Although sequential irradiation of UVA-LED and UVC induced additional bactericidal effects, simultaneous irradiation with UVA-LED and UVC induced bactericidal synergistic effects. 8-OHdG and CPDs production showed no differences between sequential and simultaneous irradiation. Interestingly, the recovery of CPDs was delayed by simultaneous irradiation. The synergistic effect was absent in SOS response-deficient mutants, such as the recA and lexA strains. Because recA- and lexA-mediated SOS responses have crucial roles in a DNA repair pathway, the synergistic bactericidal effect produced by the simultaneous irradiation could depend on suppression of the CPDs repair. The simultaneous irradiation of UVA-LED and UVC is a candidate new procedure for effective water disinfection. This article is protected by copyright. All rights reserved.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/php.12309
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25041035; ● Summary page in Scopus @ Elsevier: 2-s2.0-84915794877

(DOI: 10.1111/php.12309, PubMed: 25041035, Elsevier: Scopus) 真鍋祐矢, 前谷実希, 長野明彦, 寺西研二, 下村直行, 髙橋章 :
病原性細菌の遺伝子発現に対するパルス電界印加の影響に関する研究,
電気学会論文誌A (基礎・材料・共通部門誌), Vol.134, No.6, 390-396, 2014年.

(キーワード): パルスパワー (pulsed power) / パルス電界 / 病原性細菌 / 遺伝子発現 (gene expression) / 腸炎ビブリオ
(出版サイトへのリンク): ● Publication site (DOI): 10.1541/ieejfms.134.390
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679574730752; ● Summary page in Scopus @ Elsevier: 2-s2.0-84901780177

(DOI: 10.1541/ieejfms.134.390, CiNii: 1390282679574730752, Elsevier: Scopus) Kazuaki Mawatari, Emiko Yoshioka, Satomi Toda, Sonoko Yasui, Hiroko Furukawa, Takaaki Shimohata, Takamasa Ohnishi, Masaki Morishima, Nagakatsu Harada, Akira Takahashi, Hiroshi Sakaue and Yutaka Nakaya :
Enhancement of endothelial function inhibits left atrial thrombi development in an animal model of spontaneous left atrial thrombosis.,
Circulation Journal, Vol.78, No.8, 1980-1988, 2014.

(要約): Left atrial (LA) thrombosis is an important cause of systemic embolization. The SPORTS rat model of LA thrombi (Spontaneously-Running Tokushima-Shikoku), which have a unique characteristic of high voluntary wheel running, was previously established. The aim of the present study was to investigate how SPORTS rats develop LA thrombi.Methods and Results:Nitric oxide (NO) produced from cardiovascular endothelial cells plays an important protective role in the local regulation of blood flow, vascular tone, and platelet aggregation. No evidence of atrial fibrillation or hypercoagulability in SPORTS rats regardless of age was found; however, SPORTS rats demonstrated endothelial dysfunction and a decrease of NO production from a young age. In addition, endothelial NO synthase activity was significantly decreased in the LA and thoracic aorta endothelia of SPORTS rats. While voluntary wheel running was able to intermittently increase NO levels, running did not statistically decrease the incidence of LA thrombi at autopsy. However, L-arginine treatment significantly increased NO production and provided protection from the development of LA thrombi in SPORTS rats. They present study results indicate that NO has an important role in the development of LA thrombus, and endothelia pathways could provide new targets of therapy to prevent LA thrombosis. (Circ J 2014; 78: 1980-1988).
(出版サイトへのリンク): ● Publication site (DOI): 10.1253/circj.CJ-13-1398
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24859498; ● Summary page in Scopus @ Elsevier: 2-s2.0-84905043629

(DOI: 10.1253/circj.CJ-13-1398, PubMed: 24859498, Elsevier: Scopus) Akihiro Shirai, Mutsumi Aihara, Akira Takahashi, Hideaki Maseda and Takeshi Omasa :
Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet A light,
Journal of Photochemistry and Photobiology B: Biology, Vol.130, 226-233, 2014.

(要約): This study examined the utility of synergistic disinfection employing a gemini-quaternary ammonium salt (a gemini-QUAT, namely 3,3'-(2,7-dioxaoctane)bis(1-decylpyridinium bromide)), as an organic biocide in combination with irradiation by an ultraviolet-A (UV-A) light-emitting diode (LED) with a peak wavelength of 365nm. The combined system represents a novel disinfection method utilizing facilitated in situ oxidation depending on overproduction of reactive oxygen species (ROS) triggered by the initial action of the gemini-QUAT on the bacterial membrane. We demonstrate that this combination decreased the viability of pathogenic bacteria in a significant and rapid manner, and depended on doses of the gemini-QUAT and the fluence: the viability of Escherichia coli was reduced by greater than 5.0-logs by the combination procedure, but the decrease in viability was only 2.3-logs for exposure to UV at the same fluence dose in the absence of the gemini-QUAT. Adding catalase as a radical scavenger decreased bacterial inactivation by the combined disinfection procedure. Flow cytometric analysis indicated superoxide and hydrogen peroxide overproduction within cells treated with the combined disinfection procedure. The excessive superoxide, detected only in the combined system, appeared to be generated by the action of the gemini-QUAT at the bacterial membrane, leading to excessive and rapid generation of ROS in the system. Our data strongly suggested that this ROS promoted bacterial membrane peroxidation during initial treatment by the combination method, resulting in increased oxidative modification of DNA. These oxidative reactions may play an important role in the efficacy of this disinfection procedure.
(キーワード): Anti-Bacterial Agents / Bacteria / Bacterial Load / DNA / Lipid Peroxidation / Pyridinium Compounds / Quaternary Ammonium Compounds / Reactive Oxygen Species / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jphotobiol.2013.11.027
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24362318; ● Summary page in Scopus @ Elsevier: 2-s2.0-84890840874

(DOI: 10.1016/j.jphotobiol.2013.11.027, PubMed: 24362318, Elsevier: Scopus) Mutsumi Aihara, Xin Lian, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari, Yumi Harada, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Vegetable surface sterilization system using UVA light-emitting diodes.,
The Journal of Medical Investigation : JMI, Vol.61, No.3-4, 285-290, 2014.

(要約): Surface sterilization of fresh produce has been needed in the food manufacturing/processing industry. Here we report a UVA-LED (Ultra Violet A-Light Emitting Diode) system for surface sterilization that is safe, efficacious, low cost, and apparently harmless to fresh produce. To test the system, Escherichia coli strain DH5 was spot-inoculated onto vegetable tissues, and treated under UVA-LED. Tissues were homogenized and bacteria quantified by colony-forming assay. Possible effects of UVA-LED on vegetable quality were evaluated by HPLC. Tissue weight changes were checked after treatment at 4, 15, and 30. Bacterial inactivation by UVA-LED radiation was observed after a 10 min treatment and increased with increasing time of irradiation. The log survival ratio reached -3.23 after a 90 min treatment. Bacterial cells surviving treatment grew slowly compared to non-irradiated control cells. Cabbage tissue lost weight over time after treatment, and weight loss increased with increasing incubation temperature, but there was no difference between losses by UVA-LED treated and control tissues at any temperature tested. In addition, no differences of Vitamin C content in cabbage tissue were detected by HPLC after UVA-LED treatment. These results suggest that UVA-LED treatment has great potential for vegetable surface sterilization in the food manufacturing/processing industry.
(徳島大学機関リポジトリ): ● Metadata: 109573
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.61.285
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25264046; ● Summary page in Scopus @ Elsevier: 2-s2.0-84907509465

(徳島大学機関リポジトリ: 109573, DOI: 10.2152/jmi.61.285, PubMed: 25264046, Elsevier: Scopus) Y Kadomura-Ishikawa, Katsuyuki Miyawaki, Sumihare Noji and Akira Takahashi :
Phototropin 2 is involved in blue light-induced anthocyanin accumulation in Fragaria x ananassa fruits,
Journal of Plant Research, Vol.126, No.6, 847-857, 2013.

(要約): Anthocyanins are widespread, essential secondary metabolites in higher plants during color development in certain flowers and fruits. In strawberries, anthocyanins are also key contributors to fruit antioxidant capacity and nutritional value. However, the effects of different light qualities on anthocyanin accumulation in strawberry (Fragaria x ananassa, cv. Sachinoka) fruits remain elusive. In the present study, we showed the most efficient increase in anthocyanin content occurred by blue light irradiation. Light sensing at the molecular level was investigated by isolation of two phototropin (FaPHOT1 and FaPHOT2), two cryptochrome (FaCRY1 and FaCRY2), and two phytochrome (FaPHYA and FaPHYB) homologs. Expression analysis revealed only FaPHOT2 transcripts markedly increased depending on fruit developmental stage, and a corresponding increase in anthocyanin content was detected. FaPHOT2 knockdown resulted in decreased anthocyanin content; however, overexpression increased anthocyanin content. These findings suggested blue light induced anthocyanin accumulation, and FaPHOT2 may play a role in sensing blue light, and mediating anthocyanin biosynthesis in strawberry fruits. This is the first report to find a relationship between visible light sensing, and color development in strawberry fruits.
(キーワード): Amino Acid Sequence / Anthocyanins / Antioxidants / Down-Regulation / Flavonoids / Fragaria / Fruit / Gene Expression Regulation, Plant / Gene Knockdown Techniques / Light / Molecular Sequence Data / Phototropins / Phylogeny / Pigmentation / Sequence Alignment / Up-Regulation
(徳島大学機関リポジトリ): ● Metadata: 105905
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10265-013-0582-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23982948; ● Summary page in Scopus @ Elsevier: 2-s2.0-84886728151

(徳島大学機関リポジトリ: 105905, DOI: 10.1007/s10265-013-0582-2, PubMed: 23982948, Elsevier: Scopus) Takafumi Yakabe, Takaaki Shimohata and Akira Takahashi :
Lactobacillus brevis KB290 enhances IL-8 secretion by Vibrio parahaemolyticus-infected Caco-2 cells.,
Journal of Microbiology and Biotechnology, Vol.23, No.1, 118-124, 2013.

(要約): Vibrio parahaemolyticus in uncooked seafood causes acute gastroenteritis. The microorganism has two sets of type III secretion systems and two hemolysins. When it injects its effector proteins into a host cell via type III secretion system 1, one of the type III secretion systems induces secretion of interleukin (IL)-8, a proinflammatory chemokine, through the phosphorylation of ERK 1/2 and p38 MAPK. Although probiotics have beneficial effects on hosts and can help control some infectious diseases, there is little research on the efficacy of probiotics in V. parahaemolyticus infection. Here we pretreated V. parahaemolyticus-infected human intestinal epithelial cells with heat-killed Lactobacillus brevis KB290, a probiotic isolated from fermented vegetables (traditional Japanese pickles) and utilized as an ingredient of beverages and supplementary foods, and demonstrated its efficacy in enhancing IL-8 secretion from V. parahaemolyticus-infected cells. Among the three heat-killed lactic acid bacterial strains we tested, L. brevis KB290 induced the highest level of IL-8 secretions in the infected cells. Relative to control cells (Caco-2 cells pretreated with PBS), V. parahaemolyticus-infected Caco-2 cells pretreated with heat-killed L. brevis KB290 secreted IL-8 earlier, although concentrations were similar 450min after infection. Heat-killed L. brevis KB290 pretreatment also induced earlier ERK 1/2 phosphorylation, greater p38 MAPK phosphorylation, and enhanced IL-8 mRNA expression. Heat-killed L. brevis KB290 accelerated IL-8 secretion, a host cell immune response, in V. parahaemolyticus-infected cells. We consider this to be beneficial because IL-8 plays an important defensive role against infection, and would contribute to the repair of injured epithelial cells.
(キーワード): Caco-2 Cells / Epithelial Cells / Humans / Interleukin-8 / Lactobacillus brevis / Signal Transduction / Time Factors / Vibrio parahaemolyticus
(出版サイトへのリンク): ● Publication site (DOI): 10.4014/jmb.1207.07020
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23314377; ● Search Scopus @ Elsevier (PMID): 23314377; ● Search Scopus @ Elsevier (DOI): 10.4014/jmb.1207.07020

(DOI: 10.4014/jmb.1207.07020, PubMed: 23314377) Atsushi Hashimoto, Kazuaki Mawatari, Yohsuke Kinouchi, Masatake Akutagawa, Naotomo Ota, Kazuyuki Nishimura, Tsuyoshi Hirata and Akira Takahashi :
Inactivation of MS2 Phage and Cryptosporidium parvum Oocysts Using UV-A from High-Intensity Light-Emitting Diode for Water Disinfection,
Journal of Water and Environment Technology, Vol.11, No.4, 299-307, 2013.

(要約): In this study, high-intensity, UV-A (ranging from 360 to 370 nm, peak wavelength at 365 nm) produced by a light-emitting diode was used for the inactivation of MS2 phage and Cryptosporidium parvum oocyst. In the irradiation experiment with MS2 phage, approximately 44 and 65 J/cm2 of UV-A were required to obtain -2 and -3 log inactivations, respectively. The -2 and -3 log inactivations of Cryptosporidium oocysts required 338 and 508 J/cm2 UV-A, respectively, which were 7.7 - 7.8 times greater than those required for MS2 phage. The possibility that high-intensity UV-A irradiation can inactivate both protozoa and viruses (phage) was demonstrated in this study.
(キーワード): Cryptosporidium / inactivation / LED, MS2 phage / UV-A
(出版サイトへのリンク): ● Publication site (DOI): 10.2965/jwet.2013.299
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680154853888; ● Search Scopus @ Elsevier (DOI): 10.2965/jwet.2013.299

(DOI: 10.2965/jwet.2013.299, CiNii: 1390282680154853888) Takaaki Shimohata, Kazuaki Mawatari, Hitomi Iba, Masakazu Hamano, Sachie Negoro, Shoko Asada, Mutsumi Aihara, Akiko Hirata, Zehong Su and Akira Takahashi :
VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of Vibrio parahaemolyticus.,
Canadian Journal of Microbiology, Vol.58, No.8, 1002-1007, 2012.

(要約): Vibrio parahaemolyticus is a pathogenic Vibrio species that causes food-borne acute gastroenteritis, often related to the consumption of raw or undercooked seafood. Vibrio parahaemolyticus has 2 type III secretion systems (T3SS1 and T3SS2). Here, we demonstrate that VP1657 (VopB1) and VP1656 (VopD1), which share sequence similarity with Pseudomonas genes popB (38%) and popD (36%), respectively, are essential for translocation of T3SS1 effectors into host cells. A VP1680CyaA fusion reporter system was constructed to observe effector translocation. Using this reporter assay we showed that the VopB1 and VopD1 deletion strains were unable to translocate VP1680 to host cell but that the secretion of VP1680 into the culture medium was not affected. VopB1 or VopD1 deletion strains did not enhance cytotoxicity and failed to activate mitogen-activated protein kinases and secretion of interleukin-8, which depend on VP1680. Thus, we conclude that VopB1 and VopD1 are essential components of the translocon. To target VopB1 and VopD1 may have therapeutic potential for the treatment or prevention in V. parahaemolyticus infection.
(出版サイトへのリンク): ● Publication site (DOI): 10.1139/w2012-081
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22827847; ● Summary page in Scopus @ Elsevier: 2-s2.0-84864769777

(DOI: 10.1139/w2012-081, PubMed: 22827847, Elsevier: Scopus) Thanh Tam Thi Le, Kazuaki Mawatari, Miki Maetani, Tomomi Yamamoto, Sayaka Hayashida, Hitomi Iba, Mutsumi Aihara, Akiko Hirata, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi :
VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress.,
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1820, No.10, 1686-1692, 2012.

(要約): The V. parahaemolyticus FeSOD VP2118 may enhance ROS resistance and could promote its survival in the intestinal tract to facilitate host tissue infection.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbagen.2012.06.019
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22771831; ● Summary page in Scopus @ Elsevier: 2-s2.0-84863814713

(DOI: 10.1016/j.bbagen.2012.06.019, PubMed: 22771831, Elsevier: Scopus) Tuyet Le Thi Nhung, Hirofumi Nagata, Akira Takahashi, Mutsumi Aihara, Toshihiro Okamoto, Takaaki Shimohata, Kazuaki Mawatari, Masatake Akutagawa, Yohsuke Kinouchi and Masanobu Haraguchi :
Sterilization effect of UV light on Bacillus spores using TiO(2) films depends on wavelength.,
The Journal of Medical Investigation : JMI, Vol.59, No.1-2, 53-58, 2012.

(要約): UV light and photocatalysts such as titanium dioxide (TiO(2)) and silver (Ag) are useful for disinfection of water and surfaces. However, the effect of UV wavelength on photocatalytic disinfection of spores is not well understood. Inactivation of Bacillus spores has been examined using different UV wavelengths and TiO(2) or TiO(2)/Ag composite materials. The level of UVA disinfection of Bacillus anthracis and Bacillus brevis vegetative cells increased with the presence of the TiO(2) and Ag photocatalysts, but had little effect on their spores. B. brevis spores were slightly more sensitive to UVB and UVC than the spores of B. atrophaeus. Photocatalytic sterilization against spores was strongest in UVC and UVB and weakest in UVA. The rate of inactivation of Bacillus spores was significantly increased by the presence of TiO(2), but was not markedly different from that induced by the presence of Ag. Therefore, TiO(2)/Ag plus UVA can be used for the sterilization of vegetative cells, while TiO(2) and UVC are effective against spores.
(キーワード): Bacillus anthracis / Silver / Spores, Bacterial / 殺菌 (sterilization) / チタン (titanium) / Ultraviolet Rays
(徳島大学機関リポジトリ): ● Metadata: 106002
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.59.53
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22449993; ● Search Scopus @ Elsevier (PMID): 22449993; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.59.53

(徳島大学機関リポジトリ: 106002, DOI: 10.2152/jmi.59.53, PubMed: 22449993) Masayuki Nakano, Eiki Yamasaki, Akitoyo Ichinose, Takaaki Shimohata, Akira Takahashi, K Junko Akada, Kazuyuki Nakamura, Joel Moss, Toshiya Hirayama and Hisao Kurazono :
Salmonella enterotoxin (Stn) regulates membrane composition and integrity.,
Disease Models & Mechanisms, Vol.5, No.4, 515-521, 2012.

(要約): The mechanism of action of Salmonella enterotoxin (Stn) as a virulence factor in disease is controversial. Studies of Stn have indicated both positive and negative effects on Salmonella virulence. In this study, we attempted to evaluate Stn function and its effects on Salmonella virulence. To investigate Stn function, we first performed in vitro and in vivo analysis using mammalian cells and a murine ileal loop model. In these systems, we did not observe differences in virulence phenotypes between wild-type Salmonella and an stn gene-deleted mutant. We next characterized the phenotypes and molecular properties of the mutant strain under various in vitro conditions. The proteomic profiles of the total cell membrane protein fraction differed between wild type and mutant in that there was an absence of a protein in the mutant strain, which was identified as OmpA. By far-western blotting, OmpA was found to interact directly with Stn. To verify this result, the morphology of Salmonella was examined by transmission electron microscopy, with OmpA localization being analyzed by immunogold labeling. Compared with wild-type Salmonella, the mutant strain had a different pole structure and a thin periplasmic space; OmpA was not seen in the mutant. These results indicate that Stn, via regulation of OmpA membrane localization, functions in the maintenance of membrane composition and integrity.
(キーワード): Amino Acid Sequence / Animals / Bacterial Outer Membrane Proteins / Cell Membrane / Enterotoxins / 女性 (female) / Gene Expression Regulation, Bacterial / Genes, Bacterial / HeLa Cells / Humans / Mice / Mice, Inbred C57BL / Molecular Sequence Data / Mutation / Protein Binding / Protein Subunits / Protein Transport / RNA, Messenger / Salmonella / Sequence Alignment / U937 Cells / Virulence
(出版サイトへのリンク): ● Publication site (DOI): 10.1242/dmm.009324
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22301710; ● Search Scopus @ Elsevier (PMID): 22301710; ● Search Scopus @ Elsevier (DOI): 10.1242/dmm.009324

(DOI: 10.1242/dmm.009324, PubMed: 22301710) Nakagawa Tadahiko, Nagakatsu Harada, Miyamoto Aiko, Kawanishi Yukiko, Yoshida Masaki, Masayuki Shono, Kazuaki Mawatari, Akira Takahashi, Hiroshi Sakaue and Yutaka Nakaya :
Membrane topology of murine glycerol-3-phosphate acyltransferase 2.,
Biochemical and Biophysical Research Communications, Vol.418, No.3, 506-511, 2012.

(要約): Glycerol-3-phosphate acyltransferase (GPAT) is a rate-limiting enzyme in mammalian triacylglycerol biosynthesis. GPAT is a target for the treatment of metabolic disorders associated with high lipid accumulation. Although the molecular basis for GPAT1 activation has been investigated extensively, the activation of other isoforms, such as GPAT2, is less well understood. Here the membrane topology of the GPAT2 protein was examined using an epitope-tag-based method. Exogenously expressed GPAT2 protein was present in the membrane fraction of transformed HEK293 cells even in the presence of Na(2)CO(3) (100 mM), indicating that GPAT2 is a membrane-bound protein. Trypsin treatment of the membrane fraction degraded the N-terminal (FLAG) and C-terminal (myc-epitope) protein tags of the GPAT2 protein. Bioinformatic analysis of the GPAT2 protein sequence indicated four hydrophobic sequences as potential membrane-spanning regions (TM1-TM4). Immunoblotting of the myc-epitope tag, which was inserted between each TM region of the GPAT2 protein, showed that the amino acid sequence between TM3 and TM4 was protected from trypsin digestion. These results suggest that the GPAT2 protein has two transmembrane segments and that the N-terminal and C-terminal regions of this protein face the cytoplasm. These results also suggest that the enzymatically active motifs I-III of the GPAT2 protein face the cytosol, while motif IV is within the membrane. It is expected that the use of this topological model of GPAT2 will be essential in efforts to elucidate the molecular mechanisms of GPAT2 activity in mammalian cells.
(キーワード): Amino Acid Motifs / Animals / Cell Membrane / Cytoplasm / Glycerol-3-Phosphate O-Acyltransferase / HEK293 Cells / Humans / Mice / Molecular Sequence Data / Protein Structure, Tertiary
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2012.01.055
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22285183; ● Search Scopus @ Elsevier (PMID): 22285183; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2012.01.055

(DOI: 10.1016/j.bbrc.2012.01.055, PubMed: 22285183) Sonoko Yasui, Kazuaki Mawatari, Ran Morizumi, Hiroko Furukawa, Takaaki Shimohata, Nagakatsu Harada, Akira Takahashi and Yutaka Nakaya :
Hydrogen peroxide inhibits insulin-induced ATP-sensitive potassium channel activation independent of insulin signaling pathway in cultured vascular smooth muscle cells.,
The Journal of Medical Investigation : JMI, Vol.59, No.1-2, 36-44, 2012.

(要約): Both reactive oxygen species (ROS) and insulin resistance have been reported to play essential pathophysiological roles in cardiovascular diseases, such as hypertension and atherosclerosis. However, the mechanistic link between ROS and insulin resistance in the vasculature remains unclear. Recently we have shown that insulin causes membrane hyperpolarization via ATP-sensitive potassium (K(ATP)) channel activation, which is mediated by phosphatidylinositol 3-kinase (PI3-K) in cultured vascular smooth muscle cells (VSMCs). K(ATP) channel in the vasculature is critical in the regulation of vascular tonus. Here we examined the effects of ROS induced by hydrogen peroxide (H(2)O(2)) on insulin-induced K(ATP) channel activities in cultured VSMCs, A10 cells. H(2)O(2) (10 µM) increased significantly intercellular ROS in A10 cells. By using a cell-attached patch clamp experiment, 10 µM H(2)O(2) suppressed significantly insulin-induced K(ATP) channel activation without inhibition of insulin receptor signal transduction component including IRS and Akt in A10 cells. Furthermore 10 µM H(2)O(2) suppressed significantly pinacidil-induced K(ATP) channel activation in A10 cells. These data suggest that H(2)O(2) might inhibit directly K(ATP) channel independent of insulin signaling pathway. This study may contribute to our understanding of mechanisms of insulin resistance-associated cardiovascular disease.
(キーワード): Animals / Aorta, Thoracic / Cell Line / 過酸化水素水 (hydrogen peroxide) / Hypoglycemic Agents / インスリン (insulin) / KATP Channels / Muscle, Smooth, Vascular / Oxidants / Patch-Clamp Techniques / Rats / シグナル伝達 (signal transduction)
(徳島大学機関リポジトリ): ● Metadata: 99853
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.59.36
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22449991; ● Search Scopus @ Elsevier (PMID): 22449991; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.59.36

(徳島大学機関リポジトリ: 99853, DOI: 10.2152/jmi.59.36, PubMed: 22449991) H Nazari, A Khaleghian, Akira Takahashi, N Harada, N J. G. Webster, M Nakano, K Kishi, Y Ebina and Y Nakaya :
Cortactin, an Actin Binding Protein, Regulates GLUT4 Translocation via Actin Filament Remodeling,
Biochemistry (Moscow), Vol.76, No.11, 1262-1269, 2011.

(要約): Insulin regulates glucose uptake into fat and skeletal muscle cells by modulating the translocation of GLUT4 between the cell surface and interior. We investigated a role for cortactin, a cortical actin binding protein, in the actin filament organization and translocation of GLUT4 in Chinese hamster ovary (CHO-GLUT4myc) and L6-GLUT4myc myotube cells. Overexpression of wild-type cortactin enhanced insulin-stimulated GLUT4myc translocation but did not alter actin fiber formation. Conversely, cortactin mutants lacking the Src homology 3 (SH3) domain inhibited insulin-stimulated formation of actin stress fibers and GLUT4 translocation similar to the actin depolymerizing agent cytochalasin D. Wortmannin, genistein, and a PP1 analog completely blocked insulin-induced Akt phosphorylation, formation of actin stress fibers, and GLUT4 translocation indicating the involvement of both PI3-K/Akt and the Src family of kinases. The effect of these inhibitors was even more pronounced in the presence of overexpressed cortactin suggesting that the same pathways are involved. Knockdown of cortactin by siRNA did not inhibit insulin-induced Akt phosphorylation but completely inhibited actin stress fiber formation and glucose uptake. These results suggest that the actin binding protein cortactin is required for actin stress fiber formation in muscle cells and that this process is absolutely required for translocation of GLUT4-containing vesicles to the plasma membrane.
(キーワード): Actin Cytoskeleton / Actins / Androstadienes / Animals / CHO Cells / Cell Membrane / Cortactin / Cricetinae / Cytochalasin D / Gene Knockdown Techniques / Glucose Transporter Type 4 / Humans / Insulin / Microfilament Proteins / Muscle Fibers, Skeletal / Phosphorylation / Protein Transport / Proto-Oncogene Proteins c-akt / RNA, Small Interfering / Signal Transduction / Stress Fibers / src-Family Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1134/S0006297911110083
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22117553; ● Search Scopus @ Elsevier (PMID): 22117553; ● Search Scopus @ Elsevier (DOI): 10.1134/S0006297911110083

(DOI: 10.1134/S0006297911110083, PubMed: 22117553) Shinji Kawahito, Takashi Kawano, Hiroshi Kitahata, Jun Oto, Akira Takahashi, Kazumi Takaishi, Nagakatsu Harada, Tadahiko Nakagawa, Hiroyuki Kinoshita, Toshiharu Azma, Yutaka Nakaya and Shuzo Oshita :
Molecular mechanisms of the inhibitory effects of clonidine on vascular adenosine triphosphate-sensitive potassium channels.,
Anesthesia & Analgesia, Vol.113, No.6, 1374-1380, 2011.

(要約): We investigated the effects of the imidazoline-derived α2-adrenoceptor agonist clonidine on vascular adenosine triphosphate-sensitive potassium (K(ATP)) channel activity in rat vascular smooth muscle cells and recombinant vascular K(ATP) channels transiently expressed in COS-7 cells. Using the patch-clamp method, we investigated the effects of clonidine on the following: (1) native vascular K(ATP) channels; (2) recombinant K(ATP) channels with different combinations of various types of inwardly rectifying potassium channel (Kir6.0 family: Kir6.1, 6.2) and sulfonylurea receptor (SUR1, 2A, 2B) subunits; (3) SUR-deficient channels derived from a truncated isoform of the Kir6.2 subunit (Kir6.2ΔC36 channels); and (4) mutant Kir6.2ΔC36 channels with diminished sensitivity to ATP (Kir6.2ΔC36-K185Q channels). Clonidine (≥3 × 10(-8) M) inhibited native K(ATP) channel activity in cell-attached configurations with a half-maximal inhibitory concentration value of 1.21 × 10(-6) M and in inside-out configurations with a half-maximal inhibitory concentration value of 0.89 × 10(-6) M. With similar potency, clonidine (10(-6) or 10(-3) M) also inhibited the activities of various recombinant SUR/Kir6.0 K(ATP) channels, the Kir6.2ΔC36 channel, and the Kir6.2ΔC36-K185Q channel. Clinically relevant concentrations of clonidine inhibit K(ATP) channel activity in vascular smooth muscle cells. This inhibition seems to be the result of its effect on the Kir6.0 subunit and not on the SUR subunit.
(キーワード): Animals / COS Cells / Cell Line / Cercopithecus aethiops / Clonidine / Humans / KATP Channels / Muscle, Smooth, Vascular / Potassium Channel Blockers / Potassium Channels, Inwardly Rectifying / Rats
(出版サイトへのリンク): ● Publication site (DOI): 10.1213/ANE.0b013e3182321142
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22003223; ● Search Scopus @ Elsevier (PMID): 22003223; ● Search Scopus @ Elsevier (DOI): 10.1213/ANE.0b013e3182321142

(DOI: 10.1213/ANE.0b013e3182321142, PubMed: 22003223) Tuyet Nhung Thi Le, Hirofumi Nagata, Mutsumi Aihara, Akira Takahashi, Toshihiro Okamoto, Takaaki Shimohata, Kazuaki Mawatari, Yhosuke Kinouchi, Masatake Akutagawa and Masanobu Haraguchi :
Additional effects of silver nanoparticles on bactericidal efficiency depend on calcination temperature and dip-coating speed.,
Applied and Environmental Microbiology, Vol.77, No.16, 5629-5634, 2011.

(要約): There is an increasing interest in the application of photocatalytic properties for disinfection of surfaces, air, and water. Titanium dioxide is widely used as a photocatalyst, and the addition of silver reportedly enhances its bactericidal action. However, the synergy of silver nanoparticles and TiO(2) is not well understood. The photocatalytic elimination of Bacillus atrophaeus was examined under different calcination temperatures, dip-coating speeds, and ratios of TiO(2), SiO(2), and Ag to identify optimal production conditions for the production of TiO(2)- and/or TiO(2)/Ag-coated glass for surface disinfection. Photocatalytic disinfection of pure TiO(2) or TiO(2) plus Ag nanoparticles was dependent primarily on the calcination temperature. The antibacterial activity of TiO(2) films was optimal with a high dip-coating speed and high calcination temperature (600°C). Maximal bacterial inactivation using TiO(2)/Ag-coated glass was also observed following high-speed dip coating but with a low calcination temperature (250°C). Scanning electron microscopy (SEM) showed that the Ag nanoparticles combined together at a high calcination temperature, leading to decreased antibacterial activity of TiO(2)/Ag films due to a smaller surface area of Ag nanoparticles. The presence of Ag enhanced the photocatalytic inactivation rate of TiO(2), producing a more pronounced effect with increasing levels of catalyst loading.
(キーワード): Anti-Bacterial Agents / Bacillus / Catalysis / Disinfectants / Glass / Hot Temperature / Metal Nanoparticles / Microbial Sensitivity Tests / Microscopy, Electron, Scanning / 光化学 (photochemistry) / Silicon Dioxide / Silver / Time Factors / チタン (titanium) / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AEM.00049-11
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21724887; ● Summary page in Scopus @ Elsevier: 2-s2.0-80052609659

(DOI: 10.1128/AEM.00049-11, PubMed: 21724887, Elsevier: Scopus) Hiroko Furukawa, Kazuaki Mawatari, Kei Koyama, Sonoko Yasui, Ran Morizumi, Takaaki Shimohata, Nagakatsu Harada, Akira Takahashi and Yutaka Nakaya :
Telmisartan increases localization of glucose transporter 4 to the plasma membrane and increases glucose uptake via peroxisome proliferator-activated receptor γ in 3T3-L1 adipocytes.,
European Journal of Pharmacology, Vol.660, No.2-3, 485-491, 2011.

(要約): Angiotensin II is a peptide hormone with strong vasoconstrictive action, and recent reports have shown that Angiotensin II receptor type 1 antagonists (angiotensin II receptor blockers) also improve glucose metabolism. The angiotensin II receptor blocker telmisartan acts as an agonistic ligand of the peroxisome proliferator-activated receptor gamma (PPARγ). In this study, we investigated the effects of telmisartan on glucose uptake and insulin sensitivity in 3T3-L1 adipocytes and compared it with the action of other angiotensin II receptor blockers. Telmisartan treatment dose-dependently increased (from 1 μM) protein expression of PPARγ-regulated molecules such as fatty acid binding protein 4 (FABP4), insulin receptor, and glucose transporter 4 (GLUT4). Telmisartan increased glucose uptake both with and without insulin stimulation in 3T3-L1 adipocytes. Telmisartan increased the up-regulation of phosphorylated insulin receptor, insulin receptor substrate-1 (IRS-1) and Akt by insulin, suggesting that telmisartan increases insulin sensitivity. Furthermore, in the absence of insulin, telmisartan, but not candesartan, increased GLUT4 levels at the plasma membrane. These effects by 10 μM telmisartan were similar potency to those of 1 μM troglitazone, an activator of PPARγ. In addition, up-regulation of glucose uptake by telmisartan was inhibited by a PPARγ antagonist, T0070907 (2-chloro-5-nitro-N-4-pyridinyl-benzamide). These results indicate that telmisartan acts via PPARγ activation in adipose tissue and may be an effective therapy for the metabolic syndrome.
(キーワード): 3T3-L1 Cells / Adipocytes / Angiotensin II Type 1 Receptor Blockers / Animals / Benzimidazoles / Benzoates / Cell Membrane / Fatty Acid-Binding Proteins / Glucose / Glucose Transporter Type 4 / Insulin / Mice / PPAR gamma / Protein Transport / Receptor, Insulin / Signal Transduction
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ejphar.2011.04.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21514293; ● Search Scopus @ Elsevier (PMID): 21514293; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ejphar.2011.04.008

(DOI: 10.1016/j.ejphar.2011.04.008, PubMed: 21514293) Takaaki Shimohata, Masayuki Nakano, Xin Lian, Tomomi Shigeyama, Hitomi Iba, Akiko Hamamoto, Masaki Yoshida, Nagakatsu Harada, Hironori Yamamoto, Masayuki Yamato, Kazuaki Mawatari, Toshiaki Tamaki, Yutaka Nakaya and Akira Takahashi :
Vibrio parahaemolyticus infection induces modulation of IL-8 secretion through dual pathway via VP1680 in Caco-2 cells.,
The Journal of Infectious Diseases, Vol.203, No.4, 537-544, 2011.

(要約): Vibrio parahaemolyticus causes acute gastroenteritis and inflammations in humans. A variety of pathogenic bacteria can stimulate mitogen-activated protein kinases (MAPKs) in host cells. Phosphorylation of MAPKs leads to production of interleukin (IL)- 8 and subsequently causes inflammations. Thus, MAPK cascades were strong candidates for the main signaling pathway of V. parahaemolyticus-induced acute inflammation.
(キーワード): Caco-2 Cells / Humans / Interleukin-8 / Mitogen-Activated Protein Kinases / Phosphorylation / Signal Transduction / Vibrio parahaemolyticus / Viral Proteins / Virulence Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/infdis/jiq070
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21177635; ● Search Scopus @ Elsevier (PMID): 21177635; ● Search Scopus @ Elsevier (DOI): 10.1093/infdis/jiq070

(DOI: 10.1093/infdis/jiq070, PubMed: 21177635) A Hamamoto, M Bandou, K Nakano, Kazuaki Mawatari, Nagakatsu Harada, Masatake Akutagawa, Yohsuke Kinouchi, Yutaka Nakaya and Akira Takahashi :
Differences in stress response after UVC or UVA irradiation in Vibrio parahaemolyticus.,
Environmental Microbiology Reports, Vol.2, No.5, 660-666, 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1758-2229.2010.00154.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23766253; ● Search Scopus @ Elsevier (PMID): 23766253; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1758-2229.2010.00154.x

(DOI: 10.1111/j.1758-2229.2010.00154.x, PubMed: 23766253) Takaaki Shimohata and Akira Takahashi :
Diarrhea induced by infection of Vibrio parahaemolyticus.,
The Journal of Medical Investigation : JMI, Vol.57, No.3-4, 179-182, 2010.

(要約): Vibrio parahaemolyticus is a human pathogen that naturally inhabits marine and estuarine environments. Infection with V. parahaemolyticus is often associated with the consumption of raw or undercooked seafood, causing gastroenteritis with watery diarrhea. The presence of two type III secretion system (T3SS) proteins, thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH), has been closely associated with the severity of diarrheal illness. TDH and TRH have various biological activities including hemolytic activity, cardiotoxicity, and enterotoxicity. T3SS1 is involved in cytotoxicity to host cells and orchestrates a multifaceted host cell infection by induction of autophagy, cell rounding, and cell lysis. T3SS2 is thought to be related to the enterotoxicity of V. parahaemolyticus. The activities of inducing diarrhea of each of the virulence factors were summarized in this review.
(キーワード): Animals / Bacterial Proteins / Bacterial Secretion Systems / 細菌毒素 (bacterial toxins) / Diarrhea / Food Microbiology / Foodborne Diseases / Genes, Bacterial / Hemolysin Proteins / Humans / Seafood / Vibrio Infections / Vibrio parahaemolyticus / Virulence
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.57.179
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20847516; ● Search Scopus @ Elsevier (PMID): 20847516; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.57.179

(DOI: 10.2152/jmi.57.179, PubMed: 20847516) Takashi Kawano, Katsuya Tanaka, hua Yin, Satoru Eguchi, Hiroaki Kawano, Akira Takahashi, Yutaka Nakaya and Shuzo Oshita :
Effects of ketamine on nicorandil induced ATP-sensitive potassium channel activity in cell line derived from rat aortic smooth muscle.,
The Journal of Medical Investigation : JMI, Vol.57, No.3-4, 237-244, 2010.

(要約): Nicorandil opens adenosine triphosphate-sensitive potassium (K(ATP)) channels in the cardiovascular system and is being increasingly used for the treatment of angina pectoris. In the present study, we tested whether intravenous anesthetic agent ketamine affected nicorandil-induced native vascular K(ATP) channel activation.
(キーワード): Adenosine Diphosphate / Anesthetics, Dissociative / Animals / Cell Line / Dose-Response Relationship, Drug / Electrophysiological Phenomena / Glyburide / KATP Channels / Ketamine / Myocytes, Smooth Muscle / Nicorandil / Patch-Clamp Techniques / Rats / Stereoisomerism
(徳島大学機関リポジトリ): ● Metadata: 79136
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.57.237
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20847523; ● CiNii @ 国立情報学研究所 (CRID): 1390001204244070144; ● Search Scopus @ Elsevier (PMID): 20847523; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.57.237

(徳島大学機関リポジトリ: 79136, DOI: 10.2152/jmi.57.237, PubMed: 20847523, CiNii: 1390001204244070144) Ali Khaleghian, Gholam Hossein Riazi, Mahmoud Ghafari, Marzieh Rezaie, Akira Takahashi, Yutaka Nakaya and Hossain Nazari :
Effect of inganen anticancer properties on microtobule organization.,
Pakistan Journal of Pharmaceutical Sciences, Vol.23, No.3, 273-278, 2010.

(要約): Euphorbia tirucalli (Euphorbiaceae family) an environmental risk factor for Burkitt's lymphoma also has pharmacological activities. In the northeast of region in Brazil its latex is used as an antimicrobial, antiparasitic in the treatment of coughs, rheumatism, cancer and other disease as folk treatment. The prevalent constituents of this plant latex are diterpenes from the Inganen types (ingenol esters) as well as the tigliane (phorbol esters). Scientifically, there is not any data till now about anticancer effects of the Euphorbia tirucalli Linn., since the Ingenol esters have already presented tumor-promoting ability. Microtubules (MTs), and cytoskeletal proteins are essential in eukaryotic cells for a variety of functions, such as cellular transport, cell motility and mitosis. Single Inganen in cytoplasm can interact with these proteins and affect on their crucial functions. In this study, we showed the effects of Inganen on MT organization using ultraviolet spectrophotometer and fluorometry. The fluorescent spectroscopy showed a significant tubulin conformational change at the presence of Inganen which decrease polymerization of tubulin as well as the ultraviolet spectroscopy results. The aim of this study is to find the potential function of Inganen for treatment of cancer in cells and human organs.
(キーワード): Antineoplastic Agents, Phytogenic / Diterpenes / Euphorbia / Humans / Microtubules / Polymers / Spectrometry, Fluorescence / Tubulin
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20566439; ● Search Scopus @ Elsevier (PMID): 20566439

(PubMed: 20566439) Zehong Su, Masayuki Nakano, Tetsuro Koga, Xin Lian, Akiko Hamamoto, Takaaki Shimohata, Yumi Harada, Kazuaki Mawatari, Nagakatsu Harada, Masatake Akutagawa, Yutaka Nakaya and Akira Takahashi :
Hfq regulates anti-oxidative ability in Vibrio parahaemolyticus.,
The Journal of General and Applied Microbiology, Vol.56, No.3, 181-186, 2010.

(要約): Hfq plays a fundamental role in bacterial cell physiology. It can stimulate or repress the expression of certain target genes, and there is a possibility that Hfq regulates the oxidative stress response. However, how Hfq functions that in Vibrio parahaemolyticus remains speculative. In this paper, we explain the functions Hfq plays in V. parahaemolyticus in the gene expression of superoxide dismutase gene and catalase gene, comparing the hfq deletion mutant strain to the parental strain. The results show that the hfq deletion mutant V. parahaemolyticus has a stronger ability to resist H(2)O(2). Superoxide dismutase (SOD) and catalase (CAT) activities in the hfq deletion mutant were remarkably higher than in the parental strain. Genetic experiments indicated that the gene expression of sod and kat was up-regulated in the mutant strain. These results indicate that Hfq down-regulates CAT and SOD activity, and Hfq is associated with the oxidative stress response.
(キーワード): Catalase / Host Factor 1 Protein / Oxidative Stress / Superoxide Dismutase / Vibrio parahaemolyticus
(出版サイトへのリンク): ● Publication site (DOI): 10.2323/jgam.56.181
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20647674; ● Summary page in Scopus @ Elsevier: 2-s2.0-77954735342

(DOI: 10.2323/jgam.56.181, PubMed: 20647674, Elsevier: Scopus) Xin Lian, Kayo Tetsutani, Akiko Hamamoto, Masayuki Nakano, Kazuaki Mawatari, Nagakatsu Harada, Masayuki Yamato, Masatake Akutagawa, Yohsuke Kinouchi, Yutaka Nakaya and Akira Takahashi :
A new colored beverage disinfection system using UV-A light-emitting diodes,
Biocontrol Science, Vol.15, No.1, 33-37, 2010.

(要約): In this study we evaluated the ability of the UV-A-LED to eliminate bacteria in a colored beverage. Ten edible pigments were used to make a colored solution at concentrations of 1.0%, 0.1%, 0.01% and 0.001%. We used a colony-forming assay to monitor the bactericidal action against the bacteria. The bactericidal effect of UV-A-LED against Escherichia coli DH5 a decreased with the increasing concentration of almost all of the edible pigments. Although less effective in colored solutions and commercially available orange juice than in the positive control PBS, it holds potential for further development and use to ensure food and water safety.
(キーワード): Beverages / Colony Count, Microbial / Escherichia coli / Food Irradiation / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.4265/bio.15.33
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20361521; ● CiNii @ 国立情報学研究所 (CRID): 1390001204463296896; ● Search Scopus @ Elsevier (PMID): 20361521; ● Search Scopus @ Elsevier (DOI): 10.4265/bio.15.33

(DOI: 10.4265/bio.15.33, PubMed: 20361521, CiNii: 1390001204463296896) Masaki Yoshida, Nagakatsu Harada, Keiko Yoshida, Tadahiko Nakagawa, Takaaki Shimohata, Kazuaki Mawatari, Akira Takahashi, Hiroshi Sakaue and Yutaka Nakaya :
High density lipoprotein inhibits the activation of sterol regulatory element-binding protein-1 in cultured cells.,
FEBS Letters, Vol.584, No.6, 1217-1222, 2010.

(要約): A link between cellular uptake of high density lipoprotein (HDL) and regulation of sterol regulatory element-binding protein-1 (SREBP-1) was investigated in vitro. HDL decreased nuclear SREBP-1 levels as well as SREBP-1 target gene expression in HepG2 and HEK293 cells. However, HDL did not repress an exogenously expressed, constitutively active form of SREBP-1. HDL increased cellular cholesterol levels, and cellular cholesterol depletion by methyl-beta-cyclodextrin abolished the effects of HDL. These results suggest that HDL inhibits the activation of SREBP-1 through a cholesterol-dependent mechanism, which may play an important role in regulating lipid synthetic pathways mediated by SREBP-1.
(キーワード): Acetyl-CoA Carboxylase / Animals / Cells, Cultured / Fatty Acid Synthetase Complex / Gene Expression Regulation, Enzymologic / Hep G2 Cells / Humans / Lipid Metabolism / Lipoproteins, HDL / Male / Promoter Regions, Genetic / Rats / Rats, Sprague-Dawley / Stearoyl-CoA Desaturase / Sterol Regulatory Element Binding Protein 1 / Transcriptional Activation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.febslet.2010.02.034
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20171215; ● Summary page in Scopus @ Elsevier: 2-s2.0-77950369388

(DOI: 10.1016/j.febslet.2010.02.034, PubMed: 20171215, Elsevier: Scopus) Hattori Atsushi, Kazuaki Mawatari, Tuzuki Satomi, Yoshioka Emiko, Toda Satomi, Yoshida Masaki, Yasui Sonoko, Furukawa Hiroko, Morishima Masaki, Ono Katsushige, Takamasa Ohnishi, Nakano Masayuki, Nagakatsu Harada, Akira Takahashi and Yutaka Nakaya :
β-Adrenergic-AMPK Pathway Phosphorylates Acetyl-CoA Carboxylase in a High-epinephrine Rat Model, SPORTS,
Obesity, Vol.18, No.1, 48-54, 2010.

(要約): We established a new animal model called SPORTS (Spontaneously-Running Tokushima-Shikoku) rats, which show high-epinephrine (Epi) levels. Recent reports show that Epi activates adenosine monophosphate (AMP)-activated protein kinase (AMPK) in adipocytes. Acetyl-CoA carboxylase (ACC) is the rate-limiting enzyme in fatty acid synthesis, and the enzymatic activity is suppressed when its Ser-79 is phosphorylated by AMPK. The aim of this study was to investigate the in vivo effect of Epi on ACC and abdominal visceral fat accumulation. We divided both 6-week male control and SPORTS rats into two groups, which were fed either normal diet or high fat and sucrose (HFS) diet for 16 weeks. At the end of diet treatment, retroperitoneal fat was collected for western blotting and histological analysis. Food intake was not different among the groups, but SPORTS rats showed significantly lower weight gain than control rats in both diet groups. After 10 weeks of diet treatment, glucose tolerance tests (GTTs) revealed that SPORTS rats had increased insulin sensitivity. Furthermore, SPORTS rats had lower quantities of both abdominal fat and plasma triglyceride (TG). In abdominal fat, elevated ACC Ser-79 phosphorylation was observed in SPORTS rats and suppressed by an antagonist of beta-adrenergic receptor (AR), propranolol, or an inhibitor of AMPK, Compound C. From these results, high level of Epi induced ACC phosphorylation mediated through beta-AR and AMPK signaling pathways in abdominal visceral fat of SPORTS rats, which may contribute to reduce abdominal visceral fat accumulation and increase insulin sensitivity. Our results suggest that beta-AR-regulated ACC activity would be a target for treating lifestyle-related diseases, such as obesity.
(キーワード): AMP-Activated Protein Kinases / Acetyl-CoA Carboxylase / Adrenergic beta-Antagonists / 分散分析 (analysis of variance) / Animals / Blood Glucose / Blotting, Western / Body Weight / Eating / Enzyme-Linked Immunosorbent Assay / Epinephrine / Glucose Tolerance Test / インスリン (insulin) / Intra-Abdominal Fat / Male / 肥満症 (obesity) / リン酸化 (phosphorylation) / Propranolol / Pyrazoles / Pyrimidines / Rats / Receptors, Adrenergic, beta / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/oby.2009.145
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19444233; ● Search Scopus @ Elsevier (PMID): 19444233; ● Search Scopus @ Elsevier (DOI): 10.1038/oby.2009.145

(DOI: 10.1038/oby.2009.145, PubMed: 19444233) Gadelmoula Mostafa, Lian Xin, Maeda Miku, Aihara Mutsumi, Kazuaki Mawatari, Hamamoto Akiko, Harada Yumi, Masayuki Yamato, Masatake Akutagawa, Yutaka Nakaya, Yohsuke Kinouchi and Akira Takahashi :
Suitability of ultraviolet(A)-light emitting diode for air stream disinfection,
The Journal of Medical Investigation : JMI, Vol.56, No.3-4, 150-156, 2009.

(要約): We previously developed a high powered light-emitting diode device capable of discharging germicidal ultraviolet irradiation (UVA-LED) at an approximate wavelength of 365 nm. This study examined the bactericidal activity of UVA-LED in moving air streams. Aerosols of Escherichia coli DH5alpha were exposed to UVA-LED irradiation using a stable current (0.5 A and 1.2 mW/cm(2)) or pulse current (1.0 A and 0.2 mW/cm(2)). Settle plate analysis was used for bioaerosol sampling, where results were expressed as Colony Forming Units. A -3 Log inactivation of the E. coli population occurred after 75 minutes of constant exposure to stable current. The pulse current produced inactivation within a similar timeframe. Our results might be significant as a basic study for further investigations about the effect of UVA-LED on airborne bacteria and its suitability for air disinfection applications.
(キーワード): Aerosols / Air Microbiology / Air Pollution, Indoor / Disinfection / 大腸菌 (Escherichia coli) / Humans / Sick Building Syndrome / Ultraviolet Rays
(徳島大学機関リポジトリ): ● Metadata: 111322
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.150
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19763028; ● Search Scopus @ Elsevier (PMID): 19763028; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.150

(徳島大学機関リポジトリ: 111322, DOI: 10.2152/jmi.56.150, PubMed: 19763028) hua Yin, Nagakatsu Harada, Kazuaki Mawatari, Yasui Sonoko, Hiroko Segawa, Akira Takahashi, Shuzo Oshita and Yutaka Nakaya :
L-DOPA inhibits nitric oxide-dependent vasorelaxation via production of reactive ozygen species in rat aorta,
The Journal of Medical Investigation : JMI, Vol.56, No.3,4, 120-129, 2009.

(要約): To clarify the underlying mechanisms of L-DOPA induced vasoconstriction in rat aorta. Methods: The effect of L-DOPA on phenylephrine-induced contractile force of blood vessels was examined in vitro using rat aortic ring preparations by isometric tension experiment. Involvement of nitric oxide (NO) in the effect of L-DOPA on vascular smooth muscle was studied by using N(omega)-Nitro-L-arginine (L-NNA), Sodium nitroprusside (SNP) in endothelium-intact and endothelium-denuded aortic rings. L-DOPA potentiated alpha-adrenergic receptor- and depolarization-induced vascular contraction and inhibited acetylcholine-induced vasorelaxation. This effect was diminished by pretreatment of the aortic rings with L-NNA, an inhibitor of NO synthesis, or by removing the endothelium from the ring preparations. In endothelium-denuded rings, L-DOPA inhibited exogenous NO-dependent but not cGMP-mediated vasorelaxation. Increases in cGMP levels in response to an NO donor were attenuated by L-DOPA in cultured rat aortic smooth muscle cells. L-DOPA could not contract rings (without endothelium) pretreated with 3-(5'-hydroxymethyl- 2'-furyl)-1-benzyl indazole (YC-1), an activator of guanylyl cyclase, but SOD (150 U/ml) pretreatment of rings with endothelium inhibited contraction by L-DOPA. These results suggest that L-DOPA inhibits nitric-dependent vasorelaxation on vascular smooth muscle cells via production of reactive oxygen species.
(キーワード): Animals / Aorta, Thoracic / Cells, Cultured / Cyclic GMP / Drug Synergism / Endothelium, Vascular / Guanylate Cyclase / Levodopa / Male / Myocytes, Smooth Muscle / Nitric Oxide / Phenylephrine / Rats / Rats, Wistar / Reactive Oxygen Species / Receptors, Cytoplasmic and Nuclear / Vasodilation
(徳島大学機関リポジトリ): ● Metadata: 111318
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.120
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19763024; ● Search Scopus @ Elsevier (PMID): 19763024; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.120

(徳島大学機関リポジトリ: 111318, DOI: 10.2152/jmi.56.120, PubMed: 19763024) Masaki Yoshida, Nagakatsu Harada, Hironori Yamamoto, Yutaka Taketani, Nagakatsu Harada, Yunjie Yin, Atsushi Hattori, Tomoe Zenitani, Sayuri Hara, Haruka Yonemoto, Aki Nakamura, Masayuki Nakano, Kazuaki Mawatari, Kiyoshi Teshigawara, Hidekazu Arai, Toshio Hosaka, Akira Takahashi, Katsuhiko Yoshimoto and Yutaka Nakaya :
Identification of cis-acting promoter sequences required for expression of the glycerol-3-phosphate acyltransferase 1 gene in mice.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1791, No.1, 39-52, 2009.

(要約): Glycerol-3-phosphate acyltransferase 1 (GPAT1) is a rate limiting enzyme in de novo glycerophospholipid synthesis. The murine GPAT1 promoter sequence (the "classical" sequence) was reported previously. However, the organization of this DNA sequence does not fully match the mouse genome sequences on NCBI/GenBank. Here we have identified net cis-acting promoter sequences for the mouse GPAT1 gene: promoter 1a which includes part of the classical sequence and the downstream promoter 1b. Promoter 1a facilitates transcription of two alternative GPAT1 transcript variants, GPAT1-V1 and V2, while promoter 1b produces a third transcript variant, GPAT1-V3. Upstream stimulating factor-1 (USF-1) controlled both promoters whereas sterol regulatory element-binding protein-1 (SREBP-1) exclusively regulated promoter 1a activity in vitro. Feeding increased GPAT1-V1 and V2, but not V3 mRNA levels in mouse liver. The obese condition of db/db mice did not alter the hepatic expression levels of any of the three GPAT1 variants. Feeding enhanced hepatic mRNA levels, intranuclear protein levels and promoter 1a-binding levels of SREBP-1, but not of USF-1. Thus, promoter 1a was exclusively activated by routine feeding in vivo. Our results indicate differential roles of the two promoters in the regulation of hepatic GPAT1 gene expression in mice.
(キーワード): Animals / Base Sequence / Cell Line, Tumor / Gene Expression Regulation, Enzymologic / Glycerol-3-Phosphate O-Acyltransferase / Hepatocytes / Humans / Male / Mice / Mice, Obese / Promoter Regions, Genetic / RNA Interference / Sterol Regulatory Element Binding Protein 1 / Upstream Stimulatory Factors
(徳島大学機関リポジトリ): ● Metadata: 113301
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2008.09.005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18983939; ● Search Scopus @ Elsevier (PMID): 18983939; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2008.09.005

(徳島大学機関リポジトリ: 113301, DOI: 10.1016/j.bbalip.2008.09.005, PubMed: 18983939) M Nakano, Akira Takahashi, Z Su, Nagakatsu Harada, Kazuaki Mawatari and Yutaka Nakaya :
Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus,
BMC Microbiology, Vol.8, 155, 2008.

(要約): The hfq gene is conserved in a wide variety of bacteria and Hfq is involved in many cellular functions such as stress responses and the regulation of gene expression. It has also been reported that Hfq is involved in bacterial pathogenicity. However, it is not clear whether Hfq regulates virulence in Vibrio parahaemolyticus. To evaluate this, we investigated the effect of Hfq on the expression of virulence-associated genes including thermostable direct hemolysin (TDH), which is considered to be an important virulence factor in V. parahaemolyticus, using an hfq deletion mutant. The production of TDH in the hfq deletion mutant was much higher than in the parental strain. Quantification of tdh promoter activity and mRNA demonstrated that transcription of the tdh gene was up-regulated in the mutant strain. The hfq-complemented strain had a normal (parental) amount of tdh expression. The transcriptional activity of tdhA was particularly increased in the mutant strain. These results indicate that Hfq is closely associated with the expression level of the tdh gene. Interestingly, other genes involved in the pathogenicity of V. parahaemolyticus, such as VP1680, vopC, and vopT, were also up-regulated in the mutant strain. Hfq regulates the expression of virulence-associated factors such as TDH and may be involved in the pathogenicity of V. parahaemolyticus.
(キーワード): Amino Acid Sequence / Bacterial Proteins / Bacterial Toxins / Gene Expression Regulation, Bacterial / Hemolysin Proteins / Host Factor 1 Protein / Humans / Molecular Sequence Data / Promoter Regions, Genetic / Sequence Alignment / Transcription, Genetic / Vibrio Infections / Vibrio parahaemolyticus / Virulence Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/1471-2180-8-155
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18803872; ● Search Scopus @ Elsevier (PMID): 18803872; ● Search Scopus @ Elsevier (DOI): 10.1186/1471-2180-8-155

(DOI: 10.1186/1471-2180-8-155, PubMed: 18803872) Takashi Kawano, Katsuya Tanaka, Kazuaki Mawatari, Shuzo Oshita, Akira Takahashi and Yutaka Nakaya :
Hyperglycemia impairs isoflurane-induced adenosine triphosphate-sensitive potassium channel activation in vascular smooth muscle cells,
Anesthesia & Analgesia, Vol.106, No.3, 858-864, 2008.

(要約): Isoflurane activates vascular adenosine triphosphate sensitive potassium (K(ATP)) channels, and may induce vasodilation. In the present study, we investigated whether hyperglycemia modifies isoflurane activation of vascular K(ATP) channel. We used a cell-attached patch-clamp configuration to test the effects of isoflurane on K(ATP) channel activity in vascular smooth muscle cells (VSMCs) after incubation for 24 h in medium containing normal glucose (NG, 5.5 mM D-glucose), L-glucose (LG, 5.5 mM D-glucose plus 17.5 mM L-glucose), or high glucose (HG, 23 mM D-glucose). Superoxide levels in aortas were measured by the lucigenin-enhanced chemiluminescence technique. Isoflurane-induced open probabilities were significantly reduced in VSMCs from arteries incubated in HG (0.06 +/- 0.01) compared with NG (0.17 +/- 0.02; P < 0.05) and LG (0.15 +/- 0.02; P < 0.05). Pretreatment of VSMCs with protein kinase C (PKC) inhibitors, calphostin C and PKC inhibitor 20-28, greatly reduced HG inhibition of isoflurane-induced K(ATP) channel activity. In addition, a PKC activator, PMA, mimicked the effects of HG. Superoxide release was significantly increased in arteries incubated in HG (18.3 +/- 11.5 relative light units (RLU) x s(-1) x mg(-1); P < 0.05 versus NG). Coincubated with polyethylene glycol-superoxide dismutase (250 U/mL), a cell-permeable superoxide scavenger, greatly reduced the HG-induced increase of superoxide, but failed to reduce HG inhibition of isoflurane-induced K(ATP) channel activity. Our results suggest that the metabolic stress of hyperglycemia can impair isoflurane-induced vascular K(ATP) channel activity mediated by excessive activation of PKC. This could impede the coronary vasodilation response to isoflurane, causing ischemia or hypoxia in patients with perioperative hyperglycemia.
(キーワード): Anesthetics, Inhalation / Animals / Cells, Cultured / Enzyme Activators / Free Radical Scavengers / Glucose / Hyperglycemia / Isoflurane / KATP Channels / Male / Membrane Potentials / Muscle, Smooth, Vascular / Myocytes, Smooth Muscle / Naphthalenes / Polyethylene Glycols / Protein Kinase C / Protein Kinase Inhibitors / Rats / Rats, Wistar / Signal Transduction / Superoxide Dismutase / Superoxides / Tetradecanoylphorbol Acetate / Time Factors / Vasodilation
(出版サイトへのリンク): ● Publication site (DOI): 10.1213/ane.0b013e318163fd5b
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18292430; ● Search Scopus @ Elsevier (PMID): 18292430; ● Search Scopus @ Elsevier (DOI): 10.1213/ane.0b013e318163fd5b

(DOI: 10.1213/ane.0b013e318163fd5b, PubMed: 18292430) Nagakatsu Harada, Haruka Yonemoto, Masaki Yoshida, Hironori Yamamoto, Yunjie Yin, Aiko Miyamoto, Atsushi Hattori, Qishisan Wu, Tadahiko Nakagawa, Masayuki Nakano, Kiyoshi Teshigawara, Kazuaki Mawatari, Toshio Hosaka, Akira Takahashi and Yutaka Nakaya :
Alternative splicing produces a constitutively active form of human SREBP-1.,
Biochemical and Biophysical Research Communications, Vol.368, No.3, 820-826, 2008.

(要約): We identified a novel alternative splicing event that constitutively produces a truncated active form of human sterol regulatory element-binding protein 1 (SREBP-1). A cDNA of this splicing variant (named SREBP-1Delta) contains a translational stop codon-encoding exon sequence between exons 7 and 8. It produces SREBP-1aDelta (470 a.a.) and SREBP-1cDelta (446 a.a.) proteins that lack transmembrane and C-terminal regulatory sequences necessary for localization of SREBP-1 to the endoplasmic reticulum. A luciferase reporter assay showed that SREBP-1aDelta and SREBP-1cDelta transactivated lipogenic gene promoters to the same extent as that induced by N-terminal active fragments of SREBP-1a and SREBP-1c, respectively. SREBP-1Delta mRNA is expressed in human cell lines as well as adipose and liver tissues. Expression levels ranged from 5% to 16% of total SREBP-1 expression. The ratio of SREBP-1Delta expression to total SREBP-1 expression in HepG2 cells was not affected by either insulin or high glucose treatment.
(キーワード): Adipose Tissue / Alternative Splicing / Cell Line / Humans / Liver / Organ Specificity / Protein Isoforms / RNA Splice Sites / Sterol Regulatory Element Binding Protein 1 / Tissue Distribution
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2008.02.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18267114; ● Search Scopus @ Elsevier (PMID): 18267114; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2008.02.004

(DOI: 10.1016/j.bbrc.2008.02.004, PubMed: 18267114) Takashi Kawano, Tomohito Kawano, Katsuya Tanaka, Satoru Eguchi, Akira Takahashi, Yutaka Nakaya and Shuzo Oshita :
Effects of dopamine on ATP-sensitive potassium channels in porcine coronary artery smooth-muscle cells,
Journal of Cardiovascular Pharmacology, Vol.51, No.2, 196-201, 2008.

(要約): Dopamine is reported to be a coronary vasodilator; however, the exact mechanism of dopamine action in the coronary circulation remains unclear. In this study, we hypothesized that dopamine-induced activation of coronary ATP-sensitive potassium (KATP) channels may be associated with coronary vasodilation. We therefore investigated the direct effects of dopamine on coronary KATP-channel activity. We used patch-clamp configurations to investigate the effects of dopamine on coronary KATP-channel activity. Application of dopamine (10 to 10 M) to the bath solution during cell-attached recordings induced a concentration-dependent increase in KATP-channel activity. In contrast, dopamine failed to activate KATP channels in inside-out patches. Dopamine-induced coronary KATP-channel currents in cell-attached patches were inhibited by pretreatment with the selective D1-like antagonist, Sch-23390, but they were not influenced by the selective D2-like antagonist, domperidone, or the beta-adrenergic receptor antagonist, propranolol. The selective D1-like agonist, SKF-38393, and the adenylyl cyclase activator, forskolin, mimicked the dopamine effects on coronary KATP channels. Furthermore, pretreatment with an inhibitor of protein kinase A, Rp-cAMPS, abolished the dopamine-induced KATP-channel activation. This study demonstrates that dopamine activates coronary KATP channels via signal transduction involving the D1-like dopaminergic receptor-protein kinase A-signaling pathway.
(キーワード): 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / Animals / Benzazepines / Cardiotonic Agents / Cells, Cultured / Coronary Vessels / Cyclic AMP / Cyclic AMP-Dependent Protein Kinases / Domperidone / Dopamine / Dopamine Agonists / Dopamine Antagonists / Forskolin / KATP Channels / Myocytes, Smooth Muscle / Patch-Clamp Techniques / Propranolol / Receptors, Dopamine D1 / Signal Transduction / Swine / Thionucleotides / Vasodilator Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/FJC.0b013e31815f2be7
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18287888; ● Search Scopus @ Elsevier (PMID): 18287888; ● Search Scopus @ Elsevier (DOI): 10.1097/FJC.0b013e31815f2be7

(DOI: 10.1097/FJC.0b013e31815f2be7, PubMed: 18287888) Qishisan Wu, Nagakatsu Harada, Aki Nakamura, Masaki Yoshida, Kazuaki Mawatari, Atsushi Hattori, Qinkai Li, Takaaki Shimohata, (名) Yinhua, Xin Lian, Masayuki Nakano, Toshio Hosaka, Akira Takahashi and Yutaka Nakaya :
NO-1886, a lipoprotein lipase activator, attenuates contraction of rat intestinal ring preparations,
The Journal of Medical Investigation : JMI, Vol.55, No.1,2, 61-70, 2008.

(要約): Various intestinal symptoms or diseases are closely associated with intestinal motility, which may be altered by metabolic disturbances associated with diabetes and obesity. It is therefore important that drugs used in the treatment of metabolic disorders should not have any adverse effects on the intestine. In the present study, we examined whether [4-(4-bromo-2-cyano-phenylcarbamoyl)-benzyl]-phosphonic acid diethyl ester (NO-1886), a lipoprotein lipase activator with anti-diabetic and/or anti-obese activity, affects stimulant-induced intestinal contractility. Administration of NO-1886 to intestinal ring preparations of ileum, rectum and colon isolated from Wistar rats attenuated or relaxed contraction induced by a high K+ environment or acetylcholine (ACh). This effect of NO-1886 was dependent on extracellular Ca(2+) and intracellular myosin light chain kinase activity. Our results also showed that ACh-induced colonic contraction was significantly higher in the obese Otsuka Long-Evans Tokushima Fatty (OLETF) than in the non-obese Long-Evans Tokushima Otsuka (LETO) rats. The hypercontractility observed in the colons of OLETF rats occurred concomitantly with an elevation in muscarinic M3 ACh receptor protein levels. Administration of NO-1886 attenuated the obesity-induced hypercontractility of the colonic rings of OLETF rats. Thus, intestinal contractile system would be a novel pharmacological target of the lipoprotein lipase activator NO-1886.
(キーワード): Animals / Benzamides / Calcium / Diabetes Mellitus, Type 2 / Gastrointestinal Motility / Hypolipidemic Agents / Intestinal Diseases / Intestines / Lipoprotein Lipase Activators / Male / Muscle Contraction / Muscle, Smooth / Myosin-Light-Chain Kinase / Obesity / Organophosphorus Compounds / Peptides / Rats / Rats, Inbred OLETF / Rats, Wistar
(徳島大学機関リポジトリ): ● Metadata: 110840
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.55.61
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18319547; ● Search Scopus @ Elsevier (PMID): 18319547; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.55.61

(徳島大学機関リポジトリ: 110840, DOI: 10.2152/jmi.55.61, PubMed: 18319547) Satoru Eguchi, Takashi Kawano, hua Yin, Katsuya Tanaka, Sonoko Yasui, Kazuaki Mawatari, Akira Takahashi, Yutaka Nakaya, Shuzo Oshita and Nobuyoshi Nakajo :
Effects of prostaglandin E1 on vascular ATP-sensitive potassium channels.,
Journal of Cardiovascular Pharmacology, Vol.50, No.6, 686-691, 2007.

(要約): BACKGROUND: Prostaglandin E1 (PGE1) has been reported to activate ATP-sensitive potassium (KATP) channels, which induces vasorelaxation. However, direct evidence of PGE1 interactions with vascular KATP channels is limited. METHODS: The present study investigated the effects and mechanisms of PGE1 on vascular KATP channels in both isometric tension and patch clamp experiments.Isometric tension experiments were performed in rat thoracic aortic rings without an endothelium. Electrophysiologic experiments were performed using patch-clamp techniques to monitor KATP channels in rat vascular smooth muscle cells. RESULTS: PGE1 significantly decreased the isometric tension in a concentration-dependent manner, which was partially inhibited by pretreating with a KATP channel inhibitor, glibenclamide (1 microM), or an inhibitor of protein kinase A (PKA), Rp-cAMPS (100 microM). Application of PGE1 to the bath solution during cell-attached recordings induced a significant increase in KATP channel activity, whereas PGE1 failed to activate KATP channels in the inside-out patches. The PGE1-induced KATP channel currents in cell-attached patches were abolished by pretreating with Rp-cAMPS (100 microM). CONCLUSIONS: The results indicate that the activation of vascular KATP channels played an important role in the PKA-dependent PGE1-induced vasorelaxation. Furthermore, an electrophysiological experiment demonstrated that PGE1 activated vascular KATP channels via PKA activation.
(キーワード): Algorithms / Alprostadil / Analysis of Variance / Animals / Aorta, Thoracic / Cyclic AMP / Cyclic AMP-Dependent Protein Kinases / Dose-Response Relationship, Drug / Forskolin / Glyburide / KATP Channels / Male / Patch-Clamp Techniques / Phenylephrine / Pinacidil / Protein Kinase Inhibitors / Rats / Rats, Wistar / Thionucleotides / Vasoconstriction / Vasodilation / Vasodilator Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/FJC.0b013e3181583d9b
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18091586; ● Search Scopus @ Elsevier (PMID): 18091586; ● Search Scopus @ Elsevier (DOI): 10.1097/FJC.0b013e3181583d9b

(DOI: 10.1097/FJC.0b013e3181583d9b, PubMed: 18091586) Mirei Mori, Hamamoto Akiko, Akira Takahashi, Nakano Masayuki, Wakikawa Noriko, Tachibana Satoko, Toshitaka Ikehara, Yutaka Nakaya, Masatake Akutagawa and Yohsuke Kinouchi :
Development of a new water sterilization device with a 365 nm UV-LED,
Medical and Biological Engineering and Computing, Vol.45, No.12, 1237-1241, 2007.

(要約): 波長365nmのUV-LEDの殺菌装置への適用可能性について，他の波長の光源と比較して検討した．UVA-LEDを使った提案装置は大腸菌DH5α，腸内病原性大腸菌，腸炎ビブリオ，黄色ブドウ球菌，サルモネラ・エンテリカ血清型エンテなどの細菌を不活性化することができた．殺菌効果はUVA照射の蓄積に依存していた．UVA-LEDの安全性や省スペース性を生かし，新しい殺菌装置として使用できることが期待できる．
(キーワード): UVA-LED / Disinfection / 殺菌 (sterilization) / 細菌 (bacteria)
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11517-007-0263-1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17978842; ● Search Scopus @ Elsevier (PMID): 17978842; ● Search Scopus @ Elsevier (DOI): 10.1007/s11517-007-0263-1

(DOI: 10.1007/s11517-007-0263-1, PubMed: 17978842) Akiko Hamamoto, Mirei Mori, Akira Takahashi, Masayuki Nakano, Noriko Wakikawa, Masatake Akutagawa, Toshitaka Ikehara, Yutaka Nakaya and Yohsuke Kinouchi :
New water disinfection system using UVA-light emitting diodes.,
Journal of Applied Microbiology, Vol.103, No.6, 2291-2298, 2007.

(要約): To evaluate the ability of high-energy ultraviolet A (UVA) light-emitting diode (LED) to inactivate bacteria in water and investigate the inactivating mechanism of UVA irradiation. We developed a new disinfection device equipped with high-energy UVA-LED. Inactivation of bacteria was determined by colony-forming assay. Vibrio parahaemolyticus, enteropathogenic Escherichia coli, Staphylococcus aureus and Escherichia coli DH5alpha were reduced by greater than 5-log(10) stages within 75 min at 315 J cm(-2) of UVA. Salmonella enteritidis was reduced greater than 4-log(10) stages within 160 min at 672 J cm(-2) of UVA. The formation of 8-hydroxy-2'-deoxyguanosine in UVA-LED irradiated bacteria was 2.6-fold higher than that of UVC-irradiated bacteria at the same inactivation level. Addition of mannitol, a scavenger of hydroxyl radicals (OH(*)), or catalase, an enzyme scavenging hydrogen peroxide (H(2)O(2)) to bacterial suspensions significantly suppressed disinfection effect of UVA-LED. This disinfection system has enough ability to inactivate bacteria and OH(*) and H(2)O(2) participates in the disinfection mechanism of UVA irradiation. We newly developed UVA irradiation system and found that UVA alone was able to disinfect the water efficiently. This will become a useful disinfection system.
(キーワード): Antioxidants / 細菌 (bacteria) / Catalase / Colony-Forming Units Assay / Disinfection / Enteropathogenic Escherichia coli / 大腸菌 (Escherichia coli) / 過酸化水素水 (hydrogen peroxide) / Hydroxyl Radical / Mannitol / Staphylococcus aureus / Ultraviolet Rays / Vibrio parahaemolyticus / Water Microbiology / Water Purification
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2672.2007.03464.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18045413; ● Search Scopus @ Elsevier (PMID): 18045413; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2672.2007.03464.x

(DOI: 10.1111/j.1365-2672.2007.03464.x, PubMed: 18045413) Hirohide Yamada, Takashi Kawano, Katsuya Tanaka, Sonoko Yasui, Kazuaki Mawatari, Akira Takahashi, Yutaka Nakaya and Shuzo Oshita :
Effects of intracellular MgADP and acidification on the inhibition of cardiac sarcolemmal ATP-sensitive potassium channels by propofol,
Journal of Anesthesia, Vol.21, No.4, 472-479, 2007.

(要約): Propofol inhibits adenosine triphosphate-sensitive potassium (K(ATP)) channels, which may result in the blocking of ischemic preconditioning in the heart. During cardiac ischemia, sarcolemmal K(ATP) channel activity is regulated by the increased levels of cytosolic metabolites, such as adenosine diphosphate (ADP) and protons. However, it remains unclear whether these cytosolic metabolites modulate the inhibitory action of propofol. The aim of this study was to investigate the effects of intracellular MgADP and acidification on K(ATP) channel inhibition by propofol. We used inside-out patch-clamp configurations to investigate the effects of propofol on the activities of recombinant cardiac sarcolemmal K(ATP) channels, which are reassociated by expressed subunits, sulfonylurea receptor (SUR) 2A, and inwardly rectifying potassium channels (Kir6.2). In the absence of MgADP, propofol inhibited the SUR2A/Kir6.2 channel currents in a concentration-dependent manner, and an IC(50) of 78 microM. Increasing the intracellular MgADP concentrations to 0.1 and 0.3 mM markedly attenuated the inhibitory potency of propofol, and shifted the IC(50) to 183 and 265 microM, respectively. Moreover, decreasing the intracellular pH from 7.4 to 6.5 attenuated the inhibitory potency of propofol, and shifted the IC(50) to 277 microM. In addition, propofol-induced inhibition of truncated Kir6.2DeltaC36 currents, which form a functional channel without SUR2A, was not affected by an increase in intracellular MgADP. However, intracellular acidification (pH 6.5) significantly reduced the propofol sensitivity of Kir6.2DeltaC36 channels. Our results demonstrated that the existence of intracellular MgADP and protons attenuated the direct inhibitory potency of propofol on recombinant cardiac sarcolemmal K(ATP) channels, via SUR2A and Kir6.2 subunits, respectively.
(キーワード): ATP-Binding Cassette Transporters / Adenosine Diphosphate / Anesthetics, Intravenous / Animals / COS Cells / Cercopithecus aethiops / 心臓 (heart) / Hydrogen-Ion Concentration / KATP Channels / Potassium Channel Blockers / Potassium Channels / Potassium Channels, Inwardly Rectifying / Propofol / Receptors, Drug / Recombinant Proteins / Sarcolemma
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00540-007-0551-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18008114; ● CiNii @ 国立情報学研究所 (CRID): 1570572700582815104; ● Search Scopus @ Elsevier (PMID): 18008114; ● Search Scopus @ Elsevier (DOI): 10.1007/s00540-007-0551-9

(DOI: 10.1007/s00540-007-0551-9, PubMed: 18008114, CiNii: 1570572700582815104) Lian Xin, Akira Takahashi, Nakano Masayuki, Hori Emiko, Kazuaki Mawatari, Nagakatsu Harada, Toshio Hosaka and Yutaka Nakaya :
Vibrio parahaemolytics elevates interferon aloha production in intestinal-like epithelial Caco-2 cells,
Canadian Journal of Microbiology, Vol.53, No.9, 1084-1090, 2007.

(要約): Vibrio parahaemolyticus is the leading cause of gastroenteritis from seafood consumption. We tried to determine how the gene expression levels of intestinal-like epithelial cells (Caco-2 cells) and mouse intestinal loop mucosal cells change upon infection with this bacterium. Since we found the robust production of interferon alpha (IFN-alpha) by the V. parahaemolyticus infection, we also assessed the upregulation of a number of IFN-stimulated genes (ISGs). The expressions of IFN protein were determined by Western blotting, and the gene expressions of Caco-2 cells after V. parahaemolyticus infection were determined by quantitative real-time reverse-transcription polymerase chain reaction. Three ISGs (i.e., IFN-alpha-inducible protein 15, IFN-alpha-inducible protein 6-16, and IFN-induced protein with tetratricopeptide repeats 1) were upregulated by V. parahaemolyticus infection. Infection induced the production of IFN-alpha, but not IFN-beta or IFN-gamma. The upregulation of the 3 ISGs was suppressed by treatment with a neutralizing IFN-alpha antibody. Moreover, the production of infection-induced IFN-alpha was found in the mouse intestinal loop mucosal cells. V. parahaemolyticus infection of Caco-2 cells results in IFN-alpha production and the expression of IFN-regulated genes.
(キーワード): Animals / Caco-2 Cells / Disease Models, Animal / Epithelial Cells / Humans / Interferon-alpha / Intestines / Mice / Proteins / Up-Regulation / Vibrio Infections / Vibrio parahaemolyticus
(出版サイトへのリンク): ● Publication site (DOI): 10.1139/w07-075
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18026229; ● Search Scopus @ Elsevier (PMID): 18026229; ● Search Scopus @ Elsevier (DOI): 10.1139/w07-075

(DOI: 10.1139/w07-075, PubMed: 18026229) Masori Maria, Hamamoto Akiko, Kazuaki Mawatari, Nagakatsu Harada, Akira Takahashi and Yutaka Nakaya :
Angiotensin Decreases Glucose Uptake by Downregulation of GLUT1 in the Cell Membrene of the Vascular Smooth Muscule Cell Line A10,
Journal of Cardiovascular Pharmacology, Vol.50, No.3, 267-273, 2007.

(要約): Recent evidence suggests a crosstalk between angiotensin II (Ang II) and insulin. However, whether this crosstalk affects glucose uptake, particularly in terms of actin filament involvement, has not yet been studied in vascular smooth muscle cells. Pretreatment of cells with either Ang II or cytochalasin D disarranged actin filaments in a time-dependent manner and inhibited glucose uptake. However, insulin increased actin reorganization and glucose uptake. Membrane fractionation studies showed that Ang II decreased GLUT-1 at the cell membrane, whereas it increased GLUT-1 in the cytoplasm, indicating that Ang II may cause internalization of GLUT-1 via actin disorganization, consequently decreasing glucose uptake. The effects of Ang II on glucose uptake and actin reorganization were blocked by AT1 receptor antagonist, but not by AT2 antagonist. Either P38 or ERK1/2 inhibitors partially reversed the Ang II-inhibited actin reorganization and glucose uptake, suggesting that MAPK signaling pathways could be involved as downstream events in Ang II signaling, and this signaling may interfere with insulin-induced actin reorganization and glucose uptake. These data imply that Ang II induces insulin resistance by decreasing glucose uptake via disarrangement of actin filaments, which provides a novel insight into understanding of insulin resistance by Ang II at the molecular level.
(キーワード): Actins / Angiotensin II / Animals / Cell Fractionation / Cell Line / Cell Membrane / Cytochalasin D / Down-Regulation / Glucose / Glucose Transporter Type 1 / Insulin / Insulin Resistance / MAP Kinase Signaling System / Muscle, Smooth, Vascular / Rats / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/FJC.0b013e318093ec74
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17878754; ● Search Scopus @ Elsevier (PMID): 17878754; ● Search Scopus @ Elsevier (DOI): 10.1097/FJC.0b013e318093ec74

(DOI: 10.1097/FJC.0b013e318093ec74, PubMed: 17878754) Ono Kaori, Kazuaki Mawatari, Nagakatsu Harada, Akira Takahashi, Tohru Sakai, Ogoshi Shohei and Yutaka Nakaya :
Nucleoside and nucleotide mixture OG-VI rescues cytotoxicity induced by chemotherapeutic agents in intestinal-like epithelial cell.,
The Journal of Medical Investigation : JMI, Vol.54, No.3,4, 235-242, 2007.

(要約): Immune cells and cells undergoing rapid turn-over can obtain exogenous nucleotides via salvage synthesis. We evaluated whether or not the balanced nucleoside and nucleotide mixture OG-VI, could rescue intestinal epithelial-like Caco-2 cells from the cytotoxic effects of several chemotherapeutic agents, in the presence and absence of glutamine (Gln). Cells were exposed to 5-fluorouracil (5FU), methotrexate (MTX) or 6-mercaptopurine (6MP), after which proliferation and cell cycle analyses were performed. Following exposure to the chemotherapeutic agents, we observed that cells treated with OG-VI proliferated well, whereas those without the supplement did not proliferate. Furthermore, following treatment with either 5FU or MTX, we observed that the number of cells in the G0/G1 phase decreased and those in the S phases increased. However, these cell cycle alterations were prevented by the addition of OG-VI. With the exception of 6MP-treated cells, we did not observe any effects on proliferation or cell cycle regulation that could be ascribed to the presence of Gln. Thus, we have demonstrated that OG-VI rescues cells from the cytotoxic effects of several chemotherapeutic agents.
(キーワード): 6-Mercaptopurine / Antineoplastic Agents / Caco-2 Cells / Cell Cycle / Cell Proliferation / Cell Survival / Epithelial Cells / Fluorouracil / Humans / Intestines / Methotrexate / Nucleosides / Nucleotides
(徳島大学機関リポジトリ): ● Metadata: 111518
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.54.235
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17878671; ● Search Scopus @ Elsevier (PMID): 17878671; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.54.235

(徳島大学機関リポジトリ: 111518, DOI: 10.2152/jmi.54.235, PubMed: 17878671) Masayuki Nakano, Akira Takahashi, Y Sakai, M Kawano, Nagakatsu Harada, Kazuaki Mawatari and Yutaka Nakaya :
Catecholamine-induced stimulation of growth in Vibrio species.,
Letters in Applied Microbiology, Vol.44, No.6, 649-653, 2007.

(要約): To evaluate the effect of norepinephrine (NE) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone NE and related compounds on the growth of Vibrio parahaemolyticus and other human-pathogenic Vibrio species (Vibrio cholerae, Vibrio vulnificus, and Vibrio mimicus). The effects on bacterial growth were examined using the serum-based medium and viable cells were counted using agar plates. We have shown that NE and its related compounds stimulate growth of V. parahaemolyticus in serum-based medium. This NE-induced growth stimulation was dependent upon the presence of transferrin. NE also stimulated growth of V. mimicus, but not V. cholerae and V. vulnificus. These results suggest that the Vibrio species differ in their ability to respond to NE. It is possible that NE and related compounds modulate the pathogenicity of V. parahaemolyticus and V. mimicus.
(キーワード): Adrenergic alpha-Agonists / Catecholamines / Culture Media / Norepinephrine / Transferrin / Vibrio / Virulence
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1472-765X.2007.02136.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17576228; ● Search Scopus @ Elsevier (PMID): 17576228; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1472-765X.2007.02136.x

(DOI: 10.1111/j.1472-765X.2007.02136.x, PubMed: 17576228) Nakamura Akiyo, Shinji Kawahito, Takashi Kawano, Nazari Hossein, Akira Takahashi, Hiroshi Kitahata, Yutaka Nakaya and Shuzo Oshita :
Differential Effects of Etomidate and Midazolam on Vascular Adenosine Triphosphate-sensitive Potassium Channels: Isometric Tension and Patch Clamp Studies.,
Anesthesiology, Vol.106, No.3, 515-522, 2007.

(要約): The aim of this study was to investigate the effects of two imidazoline-derived intravenous anesthetics, etomidate and midazolam, on vascular adenosine triphosphate-sensitive potassium (KATP) channel activity. In isolated rat aorta, isometric tension was recorded to examine the anesthetic effects on vasodilator response to levcromakalim, a selective KATP channel opener. Using the patch clamp method, the anesthetic effects were also examined on the currents through (1) native vascular KATP channels, (2) recombinant KATP channels with different combinations of various types of inwardly rectifying potassium channel (Kir6.0 family: Kir6.1, 6.2) and sulfonylurea receptor (SUR1, 2A, 2B) subunits, (3) SUR-deficient channels derived from a truncated isoform of Kir6.2 subunit (Kir6.2DeltaC36 channels), and (4) mutant Kir6.2DeltaC36 channels with reduced sensitivity to adenosine triphosphate (Kir6.2DeltaC36-K185Q channels). Etomidate (> or = 10 m), but not midazolam (up to 10 m), inhibited the levcromakalim-induced vasodilation, which was sensitive to glibenclamide (IC50: 7.21 x 10 m; maximum inhibitory concentration: 1.22 x 10 m). Etomidate (> or = 3 x 10 m), but not midazolam (up to 10 m), inhibited the native KATP channel activity in both cell-attached and inside-out configurations with IC50 values of 1.68 x 10 m and 1.52 x 10 m, respectively. Etomidate (10 m) also inhibited the activity of various types of recombinant SUR/Kir6.0KATP channels, Kir6.2DeltaC36 channels, and Kir6.2DeltaC36-K185Q channels with equivalent potency. Clinical concentrations of etomidate, but not midazolam, inhibit the KATP channel activity in vascular smooth muscle cells. The inhibition is presumably through its effects on the Kir6.0 subunit, but not on the SUR subunit, with the binding site different from adenosine triphosphate at the amino acid level.
(キーワード): ATP-Binding Cassette Transporters / Adenosine Triphosphate / Anesthetics, Intravenous / Animals / Aorta, Thoracic / Cells, Cultured / Cromakalim / Dose-Response Relationship, Drug / Electrophysiology / Etomidate / Glyburide / Isometric Contraction / Male / Midazolam / Myocytes, Smooth Muscle / Organ Culture Techniques / Patch-Clamp Techniques / Potassium Channels / Potassium Channels, Inwardly Rectifying / Rats / Rats, Wistar / Receptors, Drug / Transfection / Vasodilation / Vasodilator Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200703000-00016
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17325510; ● Search Scopus @ Elsevier (PMID): 17325510; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200703000-00016

(DOI: 10.1097/00000542-200703000-00016, PubMed: 17325510) Katsuya Tanaka, Takashi Kawano, Akiyo Nakamura, Hossein Nazari, Shinji Kawahito, Shuzo Oshita, Akira Takahashi and Yutaka Nakaya :
Isoflurane activates sarcolemmal adenosine Triphosphate-sensitive potassium channels in vascular smooth muscle cells: A role of protein kinase A,
Anesthesiology, Vol.106, No.5, 984-991, 2007.

(要約): Recent evidence indicates that vascular adenosine triphosphate-sensitive potassium (K(ATP)) channels in vascular smooth muscle cells are critical in the regulation of vascular tonus under both physiologic and pathophysiologic conditions. Studies of the interaction of volatile anesthetics with vascular K(ATP) channels have been limited. In the current study, the authors investigated the molecular mechanism of isoflurane's action on vascular K(ATP) channels. Electrophysiologic experiments were performed using cell-attached and inside-out patch clamp techniques to monitor native vascular K(ATP) channels, and recombinant K(ATP) channels comprised of inwardly rectifying potassium channel subunits (Kir6.1) and the sulfonylurea receptor (SUR2B). Isometric tension experiments were performed in rat thoracic aortic rings without endothelium. Application of isoflurane (0.5 mM) to the bath solution during cell-attached recordings induced a significant increase in K(ATP) channel activity, which was greatly reduced by pretreatment with a selective inhibitor of protein kinase A (PKA), Rp-cAMPS (100 microM). In inside-out patches, isoflurane did not activate K(ATP) channels. Isoflurane significantly activated wild-type recombinant SUR2B/Kir6.1 in cell-attached patches. Isoflurane-induced activation of wild-type channels was diminished in the PKA-insensitive mutant SUR2B-T633A/Kir6.1, SUR2B-S1465A/Kir6.1, and SUR2B/Kir6.1-S385A. In addition, the authors demonstrated that isoflurane-induced PKA activation was associated with isoflurane-induced decreases in isometric tension in the rat aorta. These results indicate that isoflurane activates K(ATP) channels via PKA activation. PKA-dependent vasodilation induced by isoflurane also was observed in isometric tension experiments. Analysis of expressed vascular-type K(ATP) channels suggested that PKA-mediated phosphorylation of both Kir6.1 and SUR2B subunits plays a pivotal role in isoflurane-induced vascular K(ATP) channel activation.
(キーワード): ATP-Binding Cassette Transporters / アデノシン三リン酸 (adenosine triphosphate) / Anesthetics, Inhalation / Animals / Cyclic AMP-Dependent Protein Kinases / Isoflurane / KATP Channels / Male / Muscle, Smooth, Vascular / Potassium Channels / Potassium Channels, Inwardly Rectifying / Rats / Rats, Wistar / Receptors, Drug / Sarcolemma / Vasodilation
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/01.anes.0000265158.47556.73
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17457130; ● Search Scopus @ Elsevier (PMID): 17457130; ● Search Scopus @ Elsevier (DOI): 10.1097/01.anes.0000265158.47556.73

(DOI: 10.1097/01.anes.0000265158.47556.73, PubMed: 17457130) Nagakatsu Harada, A Kusuyama, M Morishima, Kazuko Okada, Akira Takahashi and Yutaka Nakaya :
Bezafibrate improves bacterial lipopolysaccharide-induced dyslipidemia and anorexia in rats.,
Metabolism: Clinical and Experimental, Vol.56, No.4, 517-522, 2007.

(要約): Bacterial endotoxin/lipopolysaccharide (LPS)-induced cachexia is characterized by weight loss, anorexia, and a disturbance in lipid metabolism, namely, hypertriacylglycerolemia. The aim of this study in rats with acute endotoxicity induced by an injection of LPS was to investigate whether bezafibrate, a ligand for peroxisome proliferator-activated receptor alpha and a lipoprotein lipase (LPL) activator, improved cachectic conditions, including impaired lipid metabolism. Short-term administration of LPS in the rats resulted in impairment of triacylglycerol clearance in plasma after the intake of fresh cream. In addition, LPS increased whole-body energy expenditure, reduced fasting body weight and caused anorexia in the rats. Bezafibrate treatment resulted in significant improvements in LPS-induced dyslipidemia and anorexia, but had no effect on energy expenditure, respiratory quotient, or fasting body weight in the endotoxic rats. Administration of LPS was also associated with a decrease in the level of messenger RNA (mRNA) expression for LPL in white adipose tissue and skeletal muscle and an increase in the mRNA levels for uncoupling protein 3 in skeletal muscle. Bezafibrate treatment reversed the decline in LPL mRNA levels in white adipose tissue but not in the skeletal muscle tissue of the rats. The enhanced uncoupling protein 3 mRNA level in the endotoxic rats was not affected by bezafibrate treatment. Plasma concentration of leptin was increased by short-term LPS treatment. Bezafibrate decreased the level of plasma leptin significantly without affecting the level of leptin mRNA expression. These results suggest that bezafibrate may be an effective drug not only for impaired triacylglycerol metabolism, but also for anorexia in cachectic states induced by bacterial infections.
(キーワード): Animals / Anorexia / Bezafibrate / Blood Glucose / Blood Proteins / Blotting, Northern / Body Weight / Dyslipidemias / Energy Metabolism / Hypolipidemic Agents / Ion Channels / Lipopolysaccharides / Lipoprotein Lipase / Male / Mitochondrial Proteins / Muscle Proteins / Organ Size / RNA, Messenger / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.metabol.2006.11.011
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17379010; ● Search Scopus @ Elsevier (PMID): 17379010; ● Search Scopus @ Elsevier (DOI): 10.1016/j.metabol.2006.11.011

(DOI: 10.1016/j.metabol.2006.11.011, PubMed: 17379010) Y Wang, Morishima Masaki, M Zheng, T Uchino, K Mannen, Akira Takahashi, Yutaka Nakaya, I Komuro and K Ono :
Transcription factors Csx/Nkx2.5 and GATA4 distinctly regulate expression of Ca²⁺ channels in neonatal rat heart.,
Journal of Molecular and Cellular Cardiology, Vol.42, No.6, 1045-1053, 2007.

(要約): The cardiac transcription factors Csx/Nkx2.5 and GATA4 play important roles in vertebrate heart development. Although mutations of Csx/Nkx2.5 or GATA4 are associated with various congenital heart diseases, their mechanism of action on cardiomyocyte function is not completely elucidated. In this study, we therefore investigated the actions of these transcription factors on the electrophysiological features and expression of ion channels in cardiomyocytes. Genes for transcription factors Csx/Nkx2.5 and GATA4 were transfected into rat neonatal cardiomyocytes by adenoviral infection. Action potentials, L-, T-type Ca(2+) channels and hyperpolarization-activated cation current (I(h)) of rat neonatal myocytes were recorded by patch clamp technique after adenoviral infection. Expression of ion channels was confirmed by real-time PCR. In Csx/Nkx2.5 overexpression myocytes, the spontaneous beating rate was markedly increased with an up-regulation of the Ca(v)3.2 T-type Ca(2+) channel, while in GATA4 overexpression myocytes, the T-type Ca(2+) channel was unchanged. On the other hand, the L-type Ca(2+) channel was down-regulated by both Csx/Nkx2.5 and GATA4 overexpression; the level of Ca(v)1.3 mRNA was dramatically decreased by Csx/Nkx2.5 overexpression. These results indicate that Csx/Nkx2.5 and GATA4 play important roles on the generation of pacemaker potentials modulating voltage-dependent Ca(2+) channels in the neonatal cardiomyocyte.
(キーワード): Action Potentials / Adenoviridae / Animals / Animals, Newborn / Calcium Channels, L-Type / Cells, Cultured / GATA4 Transcription Factor / Gene Expression Regulation / Heart Ventricles / Myocardium / Myocytes, Cardiac / Patch-Clamp Techniques / RNA, Messenger / Rats / Rats, Wistar / Transcription Factors / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.yjmcc.2007.03.905
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17498735; ● Search Scopus @ Elsevier (PMID): 17498735; ● Search Scopus @ Elsevier (DOI): 10.1016/j.yjmcc.2007.03.905

(DOI: 10.1016/j.yjmcc.2007.03.905, PubMed: 17498735) M Nakano, Akira Takahashi, Y Sakai and Yutaka Nakaya :
Modulation of Pathogenicity with Norepinephrine Related to the Type III Secretion System of Vibrio parahaemolyticus.,
The Journal of Infectious Diseases, Vol.195, No.9, 1353-1360, 2007.

(要約): Norepinephrine (NE) controls the functions of the gastrointestinal tract, but its role in the pathogenicity of enteropathogens is not clear. We examined the effect of NE on the pathogenicity of Vibrio parahaemolyticus with regard to its type III secretion systems (TTSSs). To evaluate the effect of NE on pathogenicity of V. parahaemolyticus, we examined the cytotoxic activity to Caco-2 cells and enterotoxicity by use of the rat ileal loop model. It has been reported that TTSS1 causes cytotoxicity and that TTSS2 causes enterotoxicity in the animal ileal loop model. Our results showed that, although NE alone did not affect the viability of Caco-2 cells, NE stimulated the cytotoxic activity of V. parahaemolyticus. Furthermore, NE increased the transcription of the TTSS1-related genes vscQ and vscU. These results indicate that NE regulates V. parahaemolyticus cytotoxic activity. The enterotoxicity of V. parahaemolyticus was increased by NE through interaction with alpha (1)-adrenergic receptors. These results indicate that alpha (1)-adrenergic receptors on the intestinal epithelium appear to interact with V. parahaemolyticus enterotoxicity. The present findings suggest that enteric NE may modulate V. parahaemolyticus pathogenicity.
(キーワード): Adrenergic alpha-Agonists / Animals / Bacterial Proteins / Caco-2 Cells / DNA Primers / Gene Expression Regulation, Bacterial / Humans / Ileum / Male / Norepinephrine / RNA, Bacterial / Rats / Rats, Wistar / Reverse Transcriptase Polymerase Chain Reaction / Vibrio Infections / Vibrio parahaemolyticus
(出版サイトへのリンク): ● Publication site (DOI): 10.1086/513275
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17397007; ● Search Scopus @ Elsevier (PMID): 17397007; ● Search Scopus @ Elsevier (DOI): 10.1086/513275

(DOI: 10.1086/513275, PubMed: 17397007) Hossein Nazari, Akira Takahashi, Nagakatsu Harada, Kazuaki Mawatari, Masayuki Nakano, Kazuhiro Kishi, Yousuke Ebina and Yutaka Nakaya :
Angiotensin II inhibits insulin-induced actin stress fiber formation and glucose uptake via ERK1/2.,
The Journal of Medical Investigation : JMI, Vol.54, No.1,2, 19-27, 2007.

(要約): There is crosstalk in intracellular signaling between Angiotensin II (Ang II) and insulin. We hypothesized that the underlying mechanism might be related to changes in cytoskeleton. In the presence of 100 nM of Ang II, insulin-induced glucose uptake was decreased and insulin-induced actin filament organization was inhibited. PKC inhibitors, including GF109203x and p38MAPK inhibitor (SB203580) neither improved insulin-induced actin reorganization nor glucose uptake. In contrast, the Ang II-induced inhibition of glucose uptake and actin filament disorganization was reversed by 10 micromol ERK 1/2 MAPK inhibitor (PD98059). Pretreatment of Ang II increased ERK1/2 phosphorylation and inhibited insulin-induced Akt phosphorylation. The effect of Ang II on ERK1/2 phosphorylation was blocked by Ang II type 1 receptor antagonists, RNH6270 and PD98059 but not by SB203580 or Guanosine-5'-O-(2-ThioDiphosphate), a G-protein inhibitor. We next tested the effect of broad-spectrum matrix metalloproteinase (MMP) inhibitor (GM6001) on Ang II-inhibition of insulin signaling pathway. GM6001 did not improve Ang II-induced actin filament disorganization and did not inhibit ERK1/2 phosphorylation. From these data in L6 myotube, we conclude that Ang II negatively regulates the insulin signal not through MMP signaling pathway but specifically through MMP-independent ERK1/2 activation pathway, providing an alternative molecular mechanism for angiotensin-induced insulin resistance.
(キーワード): Angiotensin II / Cell Line / グルコース (glucose) / Humans / インスリン (insulin) / MAP Kinase Signaling System / Mitogen-Activated Protein Kinase 1 / Mitogen-Activated Protein Kinase 3 / リン酸化 (phosphorylation) / Proto-Oncogene Proteins c-akt / Receptor, Angiotensin, Type 1 / Stress Fibers
(徳島大学機関リポジトリ): ● Metadata: 111490
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.54.19
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17380010; ● Search Scopus @ Elsevier (PMID): 17380010; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.54.19

(徳島大学機関リポジトリ: 111490, DOI: 10.2152/jmi.54.19, PubMed: 17380010) Nagakatsu Harada, S Hara, M Yoshida, T Zenitani, Kazuaki Mawatari, M Nakano, Akira Takahashi, Toshio Hosaka, Katsuhiko Yoshimoto and Yutaka Nakaya :
Molecular cloning of a murine glycerol-3-phosphate acyltransferase-like protein 1 (xGPAT1).,
Molecular and Cellular Biochemistry, Vol.297, No.1-2, 41-51, 2007.

(要約): A novel murine glycerol-3-phosphate acyltransferase-like protein 1 (named xGPAT1) has been cloned. The mouse xGPAT1 gene is located on mouse Chromosome 2, spans >19 kb, and consists of at least 23 exons. The protein is 32% identical and 72% similar to mouse mitochondrial GPAT (mtGPAT) on the amino acid level. Sequencing analysis confirmed that xGPAT1 has a 2403-bp open reading frame (ORF) that encodes an 801-amino acid protein with an estimated molecular mass of 89.1 kDa. A hydropathy plot of the deduced xGPAT1 protein showed a high degree of similarity with that of the mtGPAT protein. Using 5'-rapid amplification of cDNA ends, two alternate, untranslated exon 1 (1a and b) isoforms were obtained, generating variants xGPAT1-v1 and xGPAT1-v2. xGPAT1-v1 is expressed in mouse heart, liver, spleen, kidney and murine inner medullary collecting duct 3 (mIMCD3) cells, while xGPAT1-v2 is expressed in mouse liver, spleen, kidney, white and brown adipose tissues and 3T3-L1 pre- and post-adipocytes. xGPAT1 was distributed in the membrane fraction and showed GPAT activity when epitope-tagged xGPAT1 was expressed in Chinese hamster ovary (CHO)-K1 cells.
(キーワード): Amino Acid Sequence / Animals / Base Sequence / CHO Cells / Cell Line / Chromosomes, Mammalian / Cloning, Molecular / Cricetinae / Cricetulus / Epitopes / Exons / Gene Expression Profiling / Gene Expression Regulation, Enzymologic / Glycerol-3-Phosphate O-Acyltransferase / Introns / Mice / Mice, Inbred C57BL / Mitochondria / Molecular Sequence Data / RNA, Messenger / Sequence Homology, Amino Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11010-006-9321-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17013544; ● Search Scopus @ Elsevier (PMID): 17013544; ● Search Scopus @ Elsevier (DOI): 10.1007/s11010-006-9321-5

(DOI: 10.1007/s11010-006-9321-5, PubMed: 17013544) A Nakamura, Nagakatsu Harada, Akira Takahashi, Kazuaki Mawatari, M Nakano, K Tsutsumi and Yutaka Nakaya :
NO-1886, a lipoprotein lipase activator, attenuates vascular smooth muscle contraction in rat aorta,
European Journal of Pharmacology, Vol.554, No.2-3, 183-190, 2007.

(要約): The chemical compound [4-(4-bromo-2-cyano-phenylcarbamoyl)-benzyl]-phosphonic acid diethyl ester (NO-1886) is a lipoprotein lipase activator having beneficial effects on both diabetes control and the cardiovascular system. Preventing accumulation of lipids in the cell wall, in addition to improving insulin actions on vasculature, may indirectly contribute to the reducing effect of NO-1886 on vascular resistance. However, the direct effect of NO-1886 on vascular resistance, i.e., whether NO-1886 directly modulates the function of vascular endothelium and/or smooth muscle cells has not been investigated. In this study we therefore investigated the direct effect of NO-1886 on vascular contractility using rat aortic rings and cultured smooth muscle cell-line A10. The results show that administration of NO-1886 attenuated aortic contraction induced by phenylephrine and/or a high K(+) environment, in both the presence and absence of aortic endothelium. 1-(5-Chloronaphthalene-1-sulfonyl)homopiperazine hydrochloride (ML-9), a myosin light chain kinase (MLCK) inhibitor, blocked this inhibitory effect of NO-1886, whereas inhibitors of other signaling molecules such as calmodulin, protein kinase C and Rho-kinase had no effect. The vasorelaxant effect of NO-1886 was blocked in the absence of extracellular Ca(2+), or in the presence of the Ca(2+) channel inhibitor, verapamil. NO-1886 attenuated smooth muscle contraction induced by the cumulative addition of CaCl(2). In A10 cells, NO-1886 inhibited the membrane depolarization-induced initial peak of [Ca(2+)](i) in the presence of extracellular Ca(2+). This inhibition did not occur in the absence of extracellular Ca(2+). Taken together these results demonstrate that NO-1886 attenuates smooth muscle contraction and causes vasorelaxation by an extracellular Ca(2+)- and MLCK-dependent mechanism.
(キーワード): Adrenergic alpha-Agonists / Animals / Aorta, Thoracic / Benzamides / Calcium / Calcium Channel Blockers / Calcium Channels, N-Type / Calcium-Calmodulin-Dependent Protein Kinases / Dose-Response Relationship, Drug / Enzyme Activators / Flavonoids / Hypolipidemic Agents / Lipoprotein Lipase / Male / Muscle Contraction / Muscle, Smooth, Vascular / Organophosphorus Compounds / Phenylephrine / Potassium Chloride / Protein Kinase C / Rats / Rats, Wistar / Signal Transduction / Staurosporine / Vasoconstriction / Vasodilation / Verapamil
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ejphar.2006.09.059
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17109854; ● Search Scopus @ Elsevier (PMID): 17109854; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ejphar.2006.09.059

(DOI: 10.1016/j.ejphar.2006.09.059, PubMed: 17109854) Akira Takahashi, SI Miyoshi, N Takata, M Nakano, A Hamamoto, Kazuaki Mawatari, Nagakatsu Harada, S Shinoda and Yutaka Nakaya :
Haemolysin produced by Vibrio mimicus activates two Cl secretory pathways in cultured intestinal-like Caco-2 cells.,
Cellular Microbiology, Vol.9, No.3, 583-595, 2007.

(要約): Haemolysin (VMH) is a virulent factor produced by Vibrio mimicus, a human pathogen that causes diarrhoea. As intestinal epithelial cells are the primary targets of haemolysin, we investigated its effects on ion transport in human colonic epithelial Caco-2 cells. VMH increased the cellular short circuit current (Isc), used to estimated ion fluxes, and 125I efflux of the cells. The VMH-induced increases in Isc and 125I efflux were suppressed by depleting Ca2+ from the medium or by pretreating the cells with BAPTA-AM or by Rp-adenosin 3',5'-cyclic monophosphorothioate triethylammonium salt (Rp-cAMPS). The Cl- channel inhibitors 4,4'-disothiocyanatostibene-2,2'-disulfonic acid (DIDS), glybenclamide, and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) suppressed the VMH-induced increases in Isc and 125I efflux. Moreover, VMH increased the intracellular concentrations of Ca2+ and cAMP. Thus, VMH stimulates Caco-2 cells to secrete Cl- by activating both Ca2+ -dependent and cAMP-dependent Cl- secretion mechanisms. VMH forms ion-permeable pores in the lipid bilayer that are non-selectively permeable to small ions. However, the ion permeability of these pores was not inhibited by glybenclamide and DIDS, and VMH did not change the cell membrane potential. These observations indicate that the pores formed on the cell membrane by VMH are unlikely to be involved in VMH-induced Cl- secretion. Notably, VMH stimulated fluid accumulation in the iliac loop test that was fully suppressed by a combination of DIDS and glybenclamide. Thus, Ca2+-dependent and cAMP-dependent Cl- secretion may be important therapeutic targets with regard to the diarrhoea that is induced by Vibrio mimicus.
(キーワード): 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Animals / Bacterial Proteins / Biological Transport / Caco-2 Cells / Calcium / Chlorides / Cyclic AMP / Egtazic Acid / Glyburide / Hemolysin Proteins / Humans / Intestines / Iodine Radioisotopes / Membrane Potentials / Mice / Vibrio mimicus
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1462-5822.2006.00809.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17026482; ● Search Scopus @ Elsevier (PMID): 17026482; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1462-5822.2006.00809.x

(DOI: 10.1111/j.1462-5822.2006.00809.x, PubMed: 17026482) Akira Takahashi, Chiyo Yamamoto, Toshio Kodama, Kanami Yamashita, Nagakatsu Harada, Masayuki Nakano, Takeshi Honda and Yutaka Nakaya :
Pore formation of thermostable direct hemolysin secreted from Vibrio parahaemolyticus in lipid bilayers.,
International Journal of Toxicology, Vol.25, No.5, 409-418, 2006.

(要約): Vibrio parahaemolyticus secretes thermostable direct hemolysin (TDH), a major virulence factor. Earlier studies report that TDH is a pore-forming toxin. However, the characteristics of pores formed by TDH in the lipid bilayer, which is permeable to small ions, remain to be elucidated. Ion channel-like activities were observed in lipid bilayers containing TDH. Three types of conductance were identified. All the channels displayed relatively low ion selectivity, and similar ion permeability. The Cl- channel inhibitors, DIDS, glybenclamide, and NPPB, did not affect the channel activity of pores formed by TDH. R7, a mutant toxin of TDH, also forms pores with channel-like activity in lipid bilayers. The ion permeability of these channels is similar to that of TDH. R7 binds cultured cells and liposomes to a lower extent, compared to TDH. R7 does not display significant hemolytic activity and cell cytotoxicity, possibly owing to the difficulty of insertion into lipid membranes. Once R7 is assembled within lipid membranes, it may assume the same structure as TDH. The authors propose that the single glycine at position 62, substituted with serine in the R7 mutant toxin, plays an important role in TDH insertion into the lipid bilayer.
(キーワード): 細菌毒素 (bacterial toxins) / Caco-2 Cells / Cell Membrane Permeability / Cell Survival / Epithelial Cells / グリシン (glycine) / Hemolysin Proteins / Hemolysis / Humans / Lipid Bilayers / リポソーム (liposomes) / Vibrio parahaemolyticus
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/10915810600868181
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16940013; ● Summary page in Scopus @ Elsevier: 2-s2.0-33748645962

(DOI: 10.1080/10915810600868181, PubMed: 16940013, Elsevier: Scopus) Akira Takahashi, Nakano Masayuki, Keinosuke Okamoto, Fujii Yoshio, Kazuaki Mawatari, Nagakatsu Harada and Yutaka Nakaya :
Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO₃^-,
FEMS Microbiology Letters, Vol.258, No.1, 92-95, 2006.

(要約): Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.
(キーワード): Aeromonas / Animals / Bicarbonates / Caco-2 Cells / Chlorides / Cystic Fibrosis Transmembrane Conductance Regulator / Diarrhea / Hemolysin Proteins / Humans / Intestinal Mucosa / Mice
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1574-6968.2006.00204.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16630261; ● Search Scopus @ Elsevier (PMID): 16630261; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1574-6968.2006.00204.x

(DOI: 10.1111/j.1574-6968.2006.00204.x, PubMed: 16630261) Rie Matsushima, Akira Takahashi, Yutaka Nakaya, Hiroshi Maezawa, Mari Miki, Youichi Nakamura, Fumitaka Ohgushi and Susumu Yasuoka :
Human airway trypsin-like protease stimulates human bronchial fibroblast proliferation in a protease-activated receptor-2-dependent pathway.,
American Journal of Physiology. Lung Cellular and Molecular Physiology, Vol.290, No.2, 385-395, 2006.

(要約): Human airway trypsin-like protease (HAT) was isolated from airway secretions and localized to bronchial epithelial cells by immunohistochemistry. In the present study, we examined whether HAT could stimulate DNA synthesis and proliferation of primary human bronchial fibroblasts (HBF). HAT significantly stimulated the proliferation of HBF by 20-55%, a level similar to that of the mitogenic activity of lung mast cell tryptase (MCT). HAT also stimulated the incorporation of [3H]thymidine in HBF, and this HAT-induced DNA synthesis was abolished by leupeptin. Protease-activated receptor-2 (PAR-2) mRNA was expressed and localized to the cell surface in HBF. PAR-2 activating peptide (AP) also enhanced DNA synthesis, and both HAT and PAR-2 AP induced receptor internalization, similar to the response to trypsin. Pretreatment of HBF with anti-PAR-2 antibody significantly suppressed both HAT and PAR-2 AP-induced DNA synthesis. In addition, HAT and PAR-2 AP induced intracellular Ca2+ mobilization in HBF. The HAT-induced increase in Ca2+ was desensitized by pretreatment with trypsin or PAR-2 AP. U0126, a specific MAPK inhibitor, completely inhibited HAT-induced DNA synthesis as well as HAT-induced phosphorylation of MAPK. The effect of HAT and MCT together was additive, whereas the effect of HAT and insulin together on HBF DNA synthesis was synergistic. These results indicate that HAT stimulates fibroblast proliferation in bronchial airways through a PAR-2-dependent MEK-MAPK mediated pathway and that HAT is linked to airway processes involving fibroblasts.
(キーワード): trypsin-like protease / HAT / PAR--2 / bronchial fibroblasts
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajplung.00098.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16199437; ● Summary page in Scopus @ Elsevier: 2-s2.0-33644833762

(DOI: 10.1152/ajplung.00098.2005, PubMed: 16199437, Elsevier: Scopus) M Morishima, Nagakatsu Harada, S Hara, Atsuko Sano, Hiromasa Seno, Akira Takahashi, Yusuke Morita and Yutaka Nakaya :
Monoamine Oxidase A Activity and Norepinephrine Level in Hippocampus Determine Hyperwheel Running in SPORTS Rats.,
Neuropsychopharmacology, Vol.31, No.12, 2627-2638, 2006.

(要約): An understanding of neurological mechanisms for wheel running by rodents, especially with high exercise activity, would be applicable to a strategy for promotion of exercise motivation in humans. One of several brain regions that are candidates for the regulation of physical exercise is the hippocampus. Here we examined the running activity of Spontaneously-Running-Tokushima-Shikoku (SPORTS) rat, a new animal model for high levels of wheel-running activity, and its relation with the hippocampal norepinephrine (NE) system including the levels of NE, adrenergic receptors, and degradation enzymes for monoamines. In the hippocampus of SPORTS rats, the level of NE in extracellular fluid was augmented, whereas the level in the homogenate of the whole tissue was decreased even for sedentary conditions. Elevated extracellular NE caused downregulation of alpha(2)-adrenergic receptors in the hippocampus of SPORTS rats. Local administration of alpha(2)-adrenergic receptor antagonist yohimbine, but not of alpha(2)-agonist clonidine, into the hippocampus suppressed high running activity in SPORTS rats. The protein expression and the activity levels of monoamine oxidase A (MAOA), a critical enzyme for the degradation of NE, were decreased in the hippocampus of SPORTS rats to increase extracellular NE level. Thus, inhibition of oxidase activity in normal Wistar rats markedly increased wheel-running activity. These results indicate that decreased MAOA activity, elevation of extracellular NE, and alpha(2)-adrenergic receptors in the hippocampus determine the neural basis of the psychological regulation of exercise behavior in SPORTS rats.
(キーワード): Adrenergic alpha-2 Receptor Agonists / Adrenergic alpha-2 Receptor Antagonists / Adrenergic alpha-Agonists / Adrenergic alpha-Antagonists / Animals / Down-Regulation / Exercise Tolerance / Extracellular Fluid / Hippocampus / Locomotion / Microdialysis / Monoamine Oxidase / Monoamine Oxidase Inhibitors / Norepinephrine / Physical Conditioning, Animal / Rats / Receptors, Adrenergic, alpha-2 / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/sj.npp.1301028
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16421512; ● Summary page in Scopus @ Elsevier: 2-s2.0-33751109709

(DOI: 10.1038/sj.npp.1301028, PubMed: 16421512, Elsevier: Scopus) 森島真幸, 原小百合, 原田永勝, 佐野敦子, 妹尾廣正, 髙橋章, 中屋豊 :
高運動モデルラットSPORTSの海馬におけるノルエピネフリン動態と自発運動量,
四国医学雑誌, Vol.61, No.5,6, 185-188, 2005年.

(徳島大学機関リポジトリ): ● Metadata: 98233

(徳島大学機関リポジトリ: 98233) Yutaka Nakaya, Y Hata, K Ishida, Akira Takahashi, Kyoko Morita and Kazuhito Rokutan :
Approach to novel functional foods for stress control 2. Microarray assessment of exercise in healthy volunteers.,
The Journal of Medical Investigation : JMI, Vol.52, No.Suppl, 242-243, 2005.

(要約): DNA microarray was used to measure stress response by exercise in peripheral blood leukocytes. Aerobic exercise did not alter mRNA pattern or urinary secretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG). Strenuous exercise increased urinary secretion of 8-OHdG and altered mRNA pattern in microarray. These results suggest that moderate exercise, i. e. aerobic exercise, did not show any change in 8-OHdG, an oxidative stress marker, or mRNA expression in the leukocytes, which might reflect whole body neurohormanal changes. In addition, strenuous exercise produced quite different expression pattern from those of psychological stress.
(キーワード): Biological Markers / DNA / Deoxyguanosine / Exercise / Food / Health Status / Humans / Leukocytes, Mononuclear / Oligonucleotide Array Sequence Analysis / Physical Endurance / Physical Exertion / RNA, Messenger / Stress, Psychological / Time Factors / Voluntary Workers
(徳島大学機関リポジトリ): ● Metadata: 111556
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.52.242
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16366507; ● Search Scopus @ Elsevier (PMID): 16366507; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.52.242

(徳島大学機関リポジトリ: 111556, DOI: 10.2152/jmi.52.242, PubMed: 16366507) K Tsuchiya, I Kawamura, Akira Takahashi, T Nomura, C Kohda and M Mitsuyama :
Listeriolysin O-induced membrane permeation mediates persistent IL-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro.,
Infection and Immunity, Vol.73(7), No.7, 3869-3877, 2005.

(要約): Listeriolysin O (LLO), a major virulence factor of Listeria monocytogenes, is a member of the cholesterol-dependent cytolysin family and plays important roles not only in survival of this bacterium in phagocytes but also in induction of various cellular responses, including cytokine production. In this work, we examined the involvement of LLO in induction of the cytokine response in intestinal epithelial cells, the front line of host defense against food-borne listeriosis. Infection of Caco-2 cells with wild-type L. monocytogenes induced persistent expression of interleukin-6 (IL-6) mRNA. In contrast, IL-6 expression was observed only transiently during infection with non-LLO-producing strains. A sublytic dose of recombinant LLO (rLLO) induced the expression of IL-6 via formation of membrane pores. Under conditions of LLO-induced pore formation without extensive cell lysis, Ca2+ influx was observed, and the IL-6 expression induced by rLLO was inhibited by pretreatment with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), an intracellular Ca2+ chelator. LLO secreted by cytoplasmic L. monocytogenes appeared to induce pore formation in the membrane and to enable the trafficking of intracellular and extracellular molecules. Pretreatment with BAPTA-AM inhibited persistent IL-6 expression in Caco-2 cells infected with wild-type L. monocytogenes. These results suggest that LLO is involved in IL-6 production in the late phase of infection through the formation of Ca2+-permeable pores and subsequent Ca2+-dependent modulation of signaling and gene expression.
(キーワード): Bacterial Toxins / Caco-2 Cells / Calcium / Cell Membrane Permeability / Cytoplasm / Heat-Shock Proteins / Hemolysin Proteins / Humans / Interleukin-6 / Listeria monocytogenes / Membrane Glycoproteins / Receptors, Cell Surface / Toll-Like Receptors
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/IAI.73.7.3869-3877.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15972472; ● Search Scopus @ Elsevier (PMID): 15972472; ● Search Scopus @ Elsevier (DOI): 10.1128/IAI.73.7.3869-3877.2005

(DOI: 10.1128/IAI.73.7.3869-3877.2005, PubMed: 15972472) Akira Takahashi, N Tanoue, M Nakano, A Hamamoto, K Okamoto, Y Fujii, Nagakatsu Harada and Yutaka Nakaya :
A pore-forming toxin produced by Aeromonas sobria activates Ca²⁺ dependent Cl^- secretion.,
Microbial Pathogenesis, Vol.38, No.4, 173-180, 2005.

(要約): Bacteria produce many types of hemolysin that induce diarrhea by mechanisms that are not completely understood. Aeromonas sobria hemolysin (ASH) is a major virulence factor produced by A. sobria, a human pathogen that causes diarrhea. Since epithelial cells in the intestine are the primary targets of hemolysin, we investigated the effects of ASH on ion transport in human colonic epithelial (Caco-2) cells. ASH increased short-circuit currents (Isc) in a dose-dependent manner, and it also activated a 125I efflux from Caco-2 cells. ASH-induced Isc increases and 125I efflux activations were both suppressed by low Ca2+ levels in the extracellular solution or by pretreatment with the Ca2+ chlelator BAPTA-AM. Intracellular Ca2+ levels were increased by ASH in a biphasic fashion characterized by a rapid sharp increase (peak 1) followed by a sustained low plateau (peak 2). ASH-induced peak 1 was inhibited by pretreatment with pertussis toxin, indicating that Ca2+ was mobilized from intracellular stores, and peak 2 was induced by an influx of extracellular Ca2+. Peak 2 but not peak 1 was related to Cl- secretion. These results indicate that ASH activates Ca2+-dependent Cl- secretion.
(キーワード): Aeromonas / Caco-2 Cells / Calcium / Chelating Agents / Chlorides / Diarrhea / Egtazic Acid / Hemolysin Proteins / Humans / Ion Transport / Membrane Potentials / Pertussis Toxin
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.micpath.2005.01.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15797812; ● Search Scopus @ Elsevier (PMID): 15797812; ● Search Scopus @ Elsevier (DOI): 10.1016/j.micpath.2005.01.003

(DOI: 10.1016/j.micpath.2005.01.003, PubMed: 15797812) Masaki Morishima-Yamato, Fumiko Hisaoka, Sachiko Shinomiya, Nagakatsu Harada, Akira Takahashi, Hideki Matoba and Yutaka Nakaya :
Cloning and establishment of a line of rats for high levels of voluntary running,
Life Sciences, Vol.77, No.5, 551-561, 2005.

(要約): We generated an original Wistar line of rats that displayed increased levels of wheel running, which we named SPORTS (Spontaneously-Running-Tokushima-Shikoku). Male SPORTS rats ran voluntarily in a running wheel almost six times longer than male control Wistar rats, established without selection for their running activity. The running phenotype of female SPORTS rats was the same as female control Wistar rats. However, male offspring from the cross-mating between a female SPORTS rat and a male control rat also showed a similar level of hyper-running activity as the original SPORTS line. Compared to control rats, male SPORTS rats had lower levels of mean body weight, abdominal fat and plasma insulin after 4 weeks of running. It is likely that all these beneficial changes observed in the SPORTS rats reflected the increases in glucose disposal we observed in oral glucose tolerance tests carried out on the animals. We also found hyper-running caused a significant increase in skeletal muscle oxidative capacity, measured as the ratio of malate dehydrogenase to phosphofructokinase activity, an index of aerobic metabolism. These results indicate that the SPORTS rat may be a good animal model for determining the mechanisms responsible for up-regulation of running motivation, in addition to investigating changes in nutrient metabolism induced by high intensity exercise.
(キーワード): Animals / Blood Glucose / Breeding / Cloning, Organism / Female / Glucose Tolerance Test / Lactate Dehydrogenases / Malate Dehydrogenase / Male / Models, Animal / Motor Activity / Muscle, Skeletal / Myofibrils / Pedigree / Phosphofructokinases / Rats / Rats, Wistar / Running / Selection, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2004.10.074
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15904672; ● Search Scopus @ Elsevier (PMID): 15904672; ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2004.10.074

(DOI: 10.1016/j.lfs.2004.10.074, PubMed: 15904672) Naomi Tanoue, Akira Takahashi, Keinosuke Okamoto, Yoshio Fujii, Yutaka Taketani, Nagakatsu Harada, Masayuki Nakano and Yutaka Nakaya :
A pore-forming toxin produced by Aeromonas sobria activates cAMP-dependent Cl- secretory pathways to cause diarrhea,
FEMS Microbiology Letters, Vol.242, No.2, 195-201, 2005.

(要約): Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Cl(-) transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and (125)I efflux from Caco-2 cells, indicating ASH activate Cl(-) secretion. Additions of inhibitors of cyclic AMP dependent Cl(-) channels, glybenclamide and NPPB suppressed the Isc and (125)I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Cl(-) secretory pathway could be related with diarrhea induced by A. sobria.
(キーワード): Aeromonas / Animals / 細菌毒素 (bacterial toxins) / Caco-2 Cells / Cells, Cultured / Chloride Channels / Chlorides / Cyclic AMP / Diarrhea / Hemolysin Proteins / Humans / Intestinal Mucosa / Mice
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.femsle.2004.11.009
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15621437; ● Search Scopus @ Elsevier (PMID): 15621437; ● Search Scopus @ Elsevier (DOI): 10.1016/j.femsle.2004.11.009

(DOI: 10.1016/j.femsle.2004.11.009, PubMed: 15621437) A Wada, A-P Wang, H Isomoto, I Satomi, T Takao, Akira Takahashi, S Awata, T Nomura, Y Fujii, S Kohno, K Okamoto, J-L J Millán Moss and T Hirayama :
Placental and intestinal alkaline phosphatases are receptors for Aeromonas sobria hemolysin,
International Journal of Medical Microbiology, Vol.294, No.7, 427-435, 2005.

(要約): The Aeromonas sobria hemolysin causes diarrhea following infection by this enteropathogenic bacterium. We previously identified the putative receptor for A. sobria hemolysin as a p66 protein on Intestine 407 cells (Microb. Pathog. 27 (1999) 215-221). Here, we have partially purified and obtained a peptide mass fingerprint of p66 which revealed its identity with placental alkaline phosphatase (PLAP). Recombinant PLAP expressed in 293T cells was also found to bind to hemolysin and the binding was found not to be dependent on the N-linked glycosylation of PLAP. By immunohistochemical analysis, PLAP expression was detected in human intestinal mucosa, the target tissue in disease. In addition to PLAP, hemolysin also binds to intestinal alkaline phosphatase (IAP), an enzyme that is also abundantly expressed in intestine. Thus, both PLAP and IAP are very likely involved in the pathogenesis of diarrhea caused by this bacterial toxin.
(キーワード): Aeromonas / Alkaline Phosphatase / Amino Acid Sequence / Antigens, Neoplasm / Cell Line / GPI-Linked Proteins / Hemolysin Proteins / Humans / Intestinal Mucosa / Isoenzymes / Molecular Sequence Data / Placenta
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijmm.2004.09.012
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15715171; ● Search Scopus @ Elsevier (PMID): 15715171; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijmm.2004.09.012

(DOI: 10.1016/j.ijmm.2004.09.012, PubMed: 15715171) Takashi Kawano, Shuzo Oshita, Akira Takahashi, Y Tsutsumi, Katsuya Tanaka, Yoshinobu Tomiyama, Hiroshi Kitahata and Yutaka Nakaya :
Molecular mechanisms underlying ketamine-mediated inhibition of sarcolemmal adenosine triphosphate-sensitive potassium channels.,
Anesthesiology, Vol.102, No.1, 93-101, 2005.

(要約): Ketamine inhibits adenosine triphosphate-sensitive potassium (KATP) channels, which results in the blocking of ischemic preconditioning in the heart and inhibition of vasorelaxation induced by KATP channel openers. In the current study, the authors investigated the molecular mechanisms of ketamine's actions on sarcolemmal KATP channels that are reassociated by expressed subunits, inwardly rectifying potassium channels (Kir6.1 or Kir6.2) and sulfonylurea receptors (SUR1, SUR2A, or SUR2B). The authors used inside-out patch clamp configurations to investigate the effects of ketamine on the activities of reassociated Kir6.0/SUR channels containing wild-type, mutant, or chimeric SURs expressed in COS-7 cells. Ketamine racemate inhibited the activities of the reassociated KATP channels in a SUR subtype-dependent manner: SUR2A/Kir6.2 (IC50 = 83 microM), SUR2B/Kir6.1 (IC50 = 77 microM), SUR2B/Kir6.2 (IC50 = 89 microM), and SUR1/Kir6.2 (IC50 = 1487 microM). S-(+)-ketamine was significantly less potent than ketamine racemate in blocking all types of reassociated KATP channels. The ketamine racemate and S-(+)-ketamine both inhibited channel currents of the truncated isoform of Kir6.2 (Kir6.2DeltaC36) with very low affinity. Application of 100 mum magnesium adenosine diphosphate significantly enhanced the inhibitory potency of ketamine racemate. The last transmembrane domain of SUR2 was essential for the full inhibitory effect of ketamine racemate. These results suggest that ketamine-induced inhibition of sarcolemmal KATP channels is mediated by the SUR subunit. These inhibitory effects of ketamine exhibit specificity for cardiovascular KATP channels, at least some degree of stereoselectivity, and interaction with intracellular magnesium adenosine diphosphate.
(キーワード): ATP-Binding Cassette Transporters / Adenosine Diphosphate / Animals / COS Cells / Cercopithecus aethiops / DNA, Complementary / Excitatory Amino Acid Antagonists / KATP Channels / Ketamine / Mutation / Patch-Clamp Techniques / Potassium Channel Blockers / Potassium Channels / Potassium Channels, Inwardly Rectifying / Rats / Recombinant Fusion Proteins / Sarcolemma / Stereoisomerism / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200501000-00017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15618792; ● Search Scopus @ Elsevier (PMID): 15618792; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200501000-00017

(DOI: 10.1097/00000542-200501000-00017, PubMed: 15618792) Kazuaki Mawatari, Kakui Sae, Nagakatsu Harada, Takamasa Ohnishi, Yasuharu Niwa, Kazuko Okada, Akira Takahashi, Keisuke Izumi and Yutaka Nakaya :
Endothelin-1(1 31) levels are increased in atherosclerotic lesions of the thoracic aorta of hypercholesterolemic hamsters,
Atherosclerosis, Vol.175, No.2, 203-212, 2004.

(要約): The novel vaso-constricting 31-amino acid-length endothelin-1 [ET-1(1-31)] is selectively produced by human mast cell chymase via its action on big ET-1. However, the pathological role of ET-1(1-31) in atherosclerosis remains unclear. The aim of this study was to clarify vasoconstrictive response and expression of ET-1(1-31) in atherosclerotic aorta. Syrian golden hamster, was used for preparing the atherosclerotic models by the administration of a high cholesterol diet (HC), treatment with the nitric oxide synthase inhibitor (Nomega-nitro-L-arginine methylester, L-NAME) alone, or both (HC and L-NAME) for 40 weeks. Early atherosclerosis was observed in the case of HC or L-NAME alone treatments respectively and severe atherosclerosis was observed in the case of combined HC and L-NAME treatment. Vasoconstriction induced by ET-1(1-31) was not altered by the atherosclerotic changes, but the expression pattern of ET-1(1-31) was different at each stage of the atherosclerotic aorta. ET-1(1-31) was observed rarely in normal aortas or in early atherosclerotic lesions, but ET-1(1-31) expression was dramatically increased in aortic neointima and adventitia in a state of atherosclerosis with severe inflammation. ET-1(1-31) might play in a role of promoting atherosclerosis, and especially be involved in inflammatory mediation during the progression of atherosclerosis.
(キーワード): Animals / Aorta, Thoracic / Arteriosclerosis / 血圧 (blood pressure) / コレステロール (cholesterol) / Cricetinae / Disease Models, Animal / Endothelin-1 / Heart Rate / Hypercholesterolemia / Male / Peptide Fragments / Triglycerides / Vasoconstriction
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.atherosclerosis.2003.10.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15262175; ● Search Scopus @ Elsevier (PMID): 15262175; ● Search Scopus @ Elsevier (DOI): 10.1016/j.atherosclerosis.2003.10.015

(DOI: 10.1016/j.atherosclerosis.2003.10.015, PubMed: 15262175) Takashi Kawano, Shuzo Oshita, Akira Takahashi, Yasuo Tsutsumi, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda and Yutaka Nakaya :
Molecular Mechanisms of the Inhibitory Effects of Bupivacaine, Levobupivacaine, and Ropivacaine on Sarcolemmal Adenosine Triphosphate-sensitive Potassium Channels in the Cardiovascular System.,
Anesthesiology, Vol.101, No.2, 390-398, 2004.

(要約): Sarcolemmal adenosine triphosphate-sensitive potassium (KATP) channels in the cardiovascular system may be involved in bupivacaine-induced cardiovascular toxicity. The authors investigated the effects of local anesthetics on the activity of reconstituted KATP channels encoded by inwardly rectifying potassium channel (Kir6.0) and sulfonylurea receptor (SUR) subunits. The authors used an inside-out patch clamp configuration to investigate the effects of bupivacaine, levobupivacaine, and ropivacaine on the activity of reconstituted KATP channels expressed in COS-7 cells and containing wild-type, mutant, or chimeric SURs. Bupivacaine inhibited the activities of cardiac KATP channels (IC50 = 52 microm) stereoselectively (levobupivacaine, IC50 = 168 microm; ropivacaine, IC50 = 249 microm). Local anesthetics also inhibited the activities of channels formed by the truncated isoform of Kir6.2 (Kir6.2 delta C36) stereoselectively. Mutations in the cytosolic end of the second transmembrane domain of Kir6.2 markedly decreased both the local anesthetics' affinity and stereoselectivity. The local anesthetics blocked cardiac KATP channels with approximately eightfold higher potency than vascular KATP channels; the potency depended on the SUR subtype. The 42 amino acid residues at the C-terminal tail of SUR2A, but not SUR1 or SUR2B, enhanced the inhibitory effect of bupivacaine on the Kir6.0 subunit. Inhibitory effects of local anesthetics on KATP channels in the cardiovascular system are (1) stereoselective: bupivacaine was more potent than levobupivacaine and ropivacaine; and (2) tissue specific: local anesthetics blocked cardiac KATP channels more potently than vascular KATP channels, via the intracellular pore mouth of the Kir6.0 subunit and the 42 amino acids at the C-terminal tail of the SUR2A subunit, respectively.
(キーワード): ATP-Binding Cassette Transporters / Algorithms / Amides / Anesthetics, Local / Animals / Bupivacaine / COS Cells / Cercopithecus aethiops / Dose-Response Relationship, Drug / Membrane Potentials / Membrane Proteins / Patch-Clamp Techniques / Potassium Channel Blockers / Potassium Channels / Potassium Channels, Inwardly Rectifying / Receptors, Drug / Stereoisomerism / Sulfonylurea Compounds / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200408000-00020
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15277922; ● Search Scopus @ Elsevier (PMID): 15277922; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200408000-00020

(DOI: 10.1097/00000542-200408000-00020, PubMed: 15277922) Nagakatsu Harada, Chika Ninomiya, Yoshie Osako, Masaki Morishima, Kazuaki Mawatari, Akira Takahashi and Yutaka Nakaya :
Taurine alters respiratory gas exchange and nutrient metabolism in type 2,
Obesity Research, Vol.12, No.7, 1077-1084, 2004.

(要約): To assess the effect of taurine supplementation on respiratory gas exchange, which might reflect the improved metabolism of glucose and/or lipid in the type 2 diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Male OLETF rats (16 weeks of age) were randomly divided into two groups: unsupplemented group and taurine-supplemented (3% in drinking water) group. After 9 weeks of treatment, indirect calorimetry and insulin tolerance tests were conducted. The amounts of visceral fat pads, tissue glycogen, the blood concentrations of glucose, triacylglycerol, taurine, and electrolytes, and the level of hematocrit were compared between groups. A nondiabetic rat strain (Long-Evans Tokushima Otsuka) was used as the age-matched normal control. The indirect calorimetry showed that the treatment of OLETF rats with taurine could reduce a part of postprandial glucose oxidation possibly responsible for the increase of triacylglycerol synthesis in the body. Taurine supplementation also improved hyperglycemia and insulin resistance and increased muscle glycogen content in the OLETF rats. Supplementation with taurine increased the blood concentration of taurine and electrolyte and fluid volume, all of which were considered to be related to the improvement of metabolic disturbance in OLETF rats. Taurine supplementation may be an effective treatment for glucose intolerance and fat/lipid accumulation observed in type 2 diabetes associated with obesity. These metabolic changes might be ascribed, in part, to the alteration of circulating blood profiles, where the improved hyperglycemia and/or the blood accumulation of taurine itself would play roles.
(キーワード): Animal Nutritional Physiological Phenomena / Animals / Blood / Blood Glucose / Blood Pressure / Body Weight / Calorimetry, Indirect / Diabetes Mellitus, Type 2 / Dietary Supplements / Drinking / Eating / Electrolytes / Food / Glycogen / Hematocrit / Insulin / Insulin Resistance / Male / Osmolar Concentration / Pulmonary Gas Exchange / Rats / Rats, Inbred OLETF / Taurine
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/oby.2004.135
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15292471; ● Summary page in Scopus @ Elsevier: 2-s2.0-6344221730

(DOI: 10.1038/oby.2004.135, PubMed: 15292471, Elsevier: Scopus) Takashi Kawano, Shuzo Oshita, Akira Takahashi, Yasuo Tsutsumi, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda and Yutaka Nakaya :
Molecular mechanisms of the inhibitory effects of propofol and thiamylal on sarcolemmal adenosine triphosphate-sensitive potassium channels.,
Anesthesiology, Vol.100, No.2, 338-346, 2004.

(要約): Both propofol and thiamylal inhibit adenosine triphosphate-sensitive potassium (KATP) channels. In the current study, the authors investigated the effects of these anesthetics on the activity of recombinant sarcolemmal KATP channels encoded by inwardly rectifying potassium channel (Kir6.1 or Kir6.2) genes and sulfonylurea receptor (SUR1, SUR2A, or SUR2B) genes. The authors used inside-out patch clamp configurations to investigate the effects of propofol and thiamylal on the activity of recombinant KATP channels using COS-7 cells transfected with various types of KATP channel subunits. Propofol inhibited the activities of the SUR1/Kir6.2 (EC50 = 77 microm), SUR2A/Kir6.2 (EC50 = 72 microm), and SUR2B/Kir6.2 (EC50 = 71 microm) channels but had no significant effects on the SUR2B/Kir6.1 channels. Propofol inhibited the truncated isoform of Kir6.2 (Kir6.2DeltaC36) channels (EC50 = 78 microm) that can form functional KATP channels in the absence of SUR molecules. Furthermore, the authors identified two distinct mutations R31E (arginine residue at position 31 to glutamic acid) and K185Q (lysine residue at position 185 to glutamine) of the Kir6.2DeltaC36 channel that significantly reduce the inhibition of propofol. In contrast, thiamylal inhibited the SUR1/Kir6.2 (EC50 = 541 microm), SUR2A/Kir6.2 (EC50 = 248 microm), SUR2B/Kir6.2 (EC50 = 183 microm), SUR2B/Kir6.1 (EC50 = 170 microm), and Kir6.2DeltaC36 channels (EC50 = 719 microm). None of the mutants significantly affects the sensitivity of thiamylal. These results suggest that the major effects of both propofol and thiamylal on KATP channel activity are mediated via the Kir6.2 subunit. Site-directed mutagenesis study suggests that propofol and thiamylal may influence Kir6.2 activity by different molecular mechanisms; in thiamylal, the SUR subunit seems to modulate anesthetic sensitivity.
(キーワード): ATP-Binding Cassette Transporters / Anesthetics, Intravenous / Animals / Cells, Cultured / Diazoxide / Electrophysiology / Glyburide / Humans / Molecular Biology / Patch-Clamp Techniques / Pinacidil / Potassium Channels / Potassium Channels, Inwardly Rectifying / Propofol / Rats / Receptors, Drug / Thiamylal / Vasodilator Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200402000-00024
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14739809; ● Search Scopus @ Elsevier (PMID): 14739809; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200402000-00024

(DOI: 10.1097/00000542-200402000-00024, PubMed: 14739809) Sae Kakui, Kazuaki Mawatari, Takamasa Ohnishi, Yasuharu Niwa, Naomi Tanoue, Nagakatsu Harada, Akira Takahashi, Keisuke Izumi and Yutaka Nakaya :
Localization of the 31-amino-acid endothelin-1 in hamster tissue,
Life Sciences, Vol.74, No.11, 1435-1443, 2004.

(要約): Endothelin (ET)-1(1-31) is a novel vasoconstrictor peptide produced by human mast cell chymase, which selectively cleaves big ET-1 at the Try(31)-Gly(32) bond. We investigated the localization of ET-1(1-31) in various hamster tissues by immunohistochemistry and compared it to the distribution of ET-1(1-21). We found that the localization and amount of ET-1(1-31) were different from those of ET-1(1-21) in each tissue. ET-1(1-31)-like immunoreactivities (IR) in the heart, lung, and adrenal gland were observed in the same areas as ET-1(1-21) but were significantly weaker, suggesting that ET-1(1-31) might play a role only in mast cell/chymase-related pathological conditions in these tissues. In the liver, ET-1(1-31)-like IR was strongly detected in Kupffer cells where ET-1(1-21)-like IR was seen more weakly. In the kidney, ET-1(1-31)-like IR was slightly higher than ET-1(1-21). These results suggest that ET-1(1-31) might have physiological roles distinct from those of ET-1(1-21) in some hamster tissues.
(キーワード): Animals / Chromatography, Affinity / Chromatography, High Pressure Liquid / Chymases / Cricetinae / Endothelin-1 / Immunochemistry / Immunohistochemistry / Male / Mesocricetus / Peptide Fragments / Peptides / Serine Endopeptidases / Tissue Distribution
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2003.08.021
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14706574; ● Search Scopus @ Elsevier (PMID): 14706574; ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2003.08.021

(DOI: 10.1016/j.lfs.2003.08.021, PubMed: 14706574) S Manabe, I Kuroda, Kazuko Okada, Masaki Morishima, Nagakatsu Harada, Akira Takahashi, K Sasaki and Yutaka Nakaya :
Decreased blood levels of lactic acid and urinary excretion of 3-methylhistidine after exercise by chronic taurine treatment in rats,
Journal of Nutritional Science and Vitaminology, Vol.49, No.6, 375-380, 2003.

(要約): Taurine is reported to increase contractility of skeletal muscle and cardiac myocyte, which can increase exercise performance. The present study aimed to clarify taurine's effect on chronic endurance exercise, especially accumulation of lactic acid (LA), a marker of fatigue and ability of aerobic exercise, and urinary secretion of 3-methylhistidine (3-MH), a marker of muscle breakdown in rats. After exercise blood levels of LA and urinary excretion of 3-MH were significantly increased and this increase was significantly less in those with chronic treatment of taurine. Taurine treatment also significantly decreased fat accumulation and blood levels of cholesterol and triglyceride, which might improve insulin resistance and utilization of fat and glucose. These results indicate taurine treatment is useful for reducing physical fatigue and muscle damage during exercise training in rats, presumably due to antioxidant property and improvement of muscle and cardiac functions by taurine.
(キーワード): taurine / lactate threshold / lactic acid / 3-methylhistidine / exercise
(出版サイトへのリンク): ● Publication site (DOI): 10.3177/jnsv.49.375
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14974726; ● CiNii @ 国立情報学研究所 (CRID): 1390282681301405184; ● Search Scopus @ Elsevier (PMID): 14974726; ● Search Scopus @ Elsevier (DOI): 10.3177/jnsv.49.375

(DOI: 10.3177/jnsv.49.375, PubMed: 14974726, CiNii: 1390282681301405184) Mari Miki, Yoichi Nakamura, Akira Takahashi, Yutaka Nakaya, Hiroshi Eguchi, Tsukio Masegi, Kazuo Yoneda, Susumu Yasuoka and Saburo Sone :
Effect of human airway trypsin-like protease on intracellular free Ca²⁺ concentration in human bronchial epithelial cells,
The Journal of Medical Investigation : JMI, Vol.50, No.1-2, 95-107, 2003.

(要約): It has been shown that human airway trypsin-like protease (HAT) is localized in human bronchial epithelial cells (HBEC), and trypsin activates protease-activated receptor-2 (PAR-2). Activation of PAR-2 activates G-protein followed by an increase of intracellular free Ca2+, [Ca2+]in. This study was undertaken to clarify whether HAT can activate PAR-2 in HBEC or not. RT-PCR showed that HAT mRNA is expressed in HBEC, and PAR-2 mRNA is the most strongly expressed of the known PARs in HBEC. Both PAR-2 agonist peptide (PAR-2 AP) and HAT increased [Ca2+]in in HBEC in a biphasic fashion; a prompt, sharp increase (peak I) and a sustained low plateau (peak II). PAR-2 AP over 100-200 microM and HAT over 200-300 mU/ml (0.08-0.12 microM) induced both peak I and II, and PAR-2 AP below 100 microM and HAT below 200 mU/ml induced only peak II. Both PAR-2 AP-induced and HAT-induced peak I were induced by Ca2+ mobilization from intracellular stores, because they appeared even in Ca2+-free medium. Both PAR-2 AP-induced and HAT-induced peak II were induced by an influx of extracellular Ca2+, because they were abolished in Ca2+-free medium. The Ca2+ response to HAT was desensitized by exposure of HBEC to PAR-2 AP. These results indicate that HBEC have a functional PAR-2, and HAT regulates cellular functions of HBEC via activation of PAR-2.
(キーワード): Adult / Bronchi / Calcium / Calcium Signaling / Cell Line, Transformed / Cells, Cultured / Culture Media / Enzyme Induction / Epithelial Cells / Fibroblasts / Humans / Intracellular Fluid / Ion Transport / Lung / Macrophages, Alveolar / Peptide Fragments / RNA, Messenger / Receptor, PAR-1 / Receptor, PAR-2 / Receptors, Thrombin / Recombinant Fusion Proteins / Serine Endopeptidases
(徳島大学機関リポジトリ): ● Metadata: 110683
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12630574; ● Search Scopus @ Elsevier (PMID): 12630574

(徳島大学機関リポジトリ: 110683, PubMed: 12630574) Yutaka Nakaya, Nagakatsu Harada, Sae Kakui, Kazuko Okada, Akira Takahashi, Junnya Inoi and Susumu Ito :
Severe catabolic state after prolonged fasting in cirrhotic patients: effect of oral branched-chain amino-acid-enriched nutrient mixture,
Journal of Gastroenterology, Vol.37, No.7, 531-536, 2002.

(要約): Cirrhotic patients frequently undergo various medical procedures, such as diagnostic gastrointestinal endoscopy, without taking breakfast. The aim of the present study was to clarify the effect of longer fasting (> 12 h) on energy metabolism, and to test whether supplementation of an oral branched-chain amino-acid-enriched nutrient mixture (BCAA mixture), which contains various nutrients in addition to BCAA, could improve the catabolic state. Metabolic measurement was performed in 30 cirrhotic patients and 13 normal subjects, using indirect calorimetry. Compared with that in the normal subjects, the respiratory quotient (RQ) was significantly lower after an overnight fast in the cirrhotic patients, indicating accelerated fat oxidation and a catabolic state. In addition, RQ in cirrhotic patients (n = 7) decreased rapidly with longer fasting, whereas that in the normal subjects (n = 5) showed relatively stable values. These results indicate that special care should be taken with medical procedures that are carried out in patients who have fasted. The effect of oral glucose, a carbohydrate-rich snack (rice ball), and the BCAA mixture (each, 210 kcal) on RQ was studied in 6 normal subjects and 6 patients with liver cirrhosis after an overnight fast. Supplementation of the carbohydrate-rich snack and the BCAA mixture (210 kcal each) elevated RQ and blood glucose levels to a similar degree in the cirrhotic patients. Oral administration of glucose (210 kcal) led to significantly greater elevation of blood glucose levels than the other snacks, which may be unfavorable for cirrhotic patients, who frequently have glucose intolerance. In the 30 cirrhotic patients, supplementation with the BCAA mixture in the late evening significantly improved RQ in the early morning. Carbohydrate-rich meals are used as a late evening snack in cirrhotic patients, but our study indicates that supplementation with a BCAA mixture can also be used to reduce fat oxidation in the early morning, with results similar to those with carbohydrate-rich snacks.
(キーワード): Administration, Oral / Amino Acids, Branched-Chain / Blood Glucose / Calorimetry, Indirect / Case-Control Studies / エネルギー代謝 (energy metabolism) / Fasting / Female / Food / Humans / Liver Cirrhosis / Male / Middle Aged / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s005350200082
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12162411; ● Search Scopus @ Elsevier (PMID): 12162411; ● Search Scopus @ Elsevier (DOI): 10.1007/s005350200082

(DOI: 10.1007/s005350200082, PubMed: 12162411) Manabu Kinoshita, Yutaka Nakaya, Nagakatsu Harada, Akira Takahashi, Masahiro Nomura and Shigenobu Bando :
Combination Therapy of Exercise and Angiotensin-Converting Enzyme Inhibitor Markedly Improves Insulin Sensitivities in Hypertensive Patients With Insulin Resistance,
Circulation Journal, Vol.66, No.7, 655-658, 2002.

(要約): The contraction of muscle enhances the release of bradykinin (BK) and improves glucose uptake by the muscle. Angiotensin-converting enzyme inhibitor (ACEI) slows the breakdown of BK, thus the effect of BK is augmented in the presence of ACEI. The present study investigated whether the combination of exercise (increased production of BK) and ACEI (delay in breakdown of BK) might further improve insulin sensitivity in hypertensive patients with insulin resistance (HOMA-R>1.8). Patients were assigned either to increased walking distance (Walking group) or taking 2mg remocapril, an ACEI, daily (ACEI group) for 8 weeks. Then both interventions were given to all patients for 8 weeks (ACEI+Walking group). Blood concentrations of triglycerides were slightly lower in the ACEI+Walking group than at baseline, although there were no significant differences in total cholesterol or high density lipoprotein-cholesterol among the 2 groups. Blood glucose was not significantly different with each treatment, but blood concentrations of insulin and HOMA-R were significantly lower in the Walking and ACEI groups compared with the Control group. The combination of walking and ACEI further lowered blood concentrations of insulin and HOMA-R, which suggests that this treatment is beneficial for hypertensive patients with insulin resistance.
(キーワード): Bradykinin / Insulin resistance / Temocapril / Walking
(出版サイトへのリンク): ● Publication site (DOI): 10.1253/circj.66.655
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680082585984; ● Search Scopus @ Elsevier (DOI): 10.1253/circj.66.655

(DOI: 10.1253/circj.66.655, CiNii: 1390282680082585984) Takashi Kawano, Shuzo Oshita, Yasuo Tsutsumi, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda, Akira Takahashi and Yutaka Nakaya :
Clinically relevant concentrations of propofol have no effect on adenosine triphosphate-sensitive potassium channels in rat ventricular myocytes.,
Anesthesiology, Vol.96, No.6, 1472-1477, 2002.

(要約): Activation of adenosine triphosphate-sensitive potassium (K(ATP)) channels produces cardioprotective effects during ischemia. Because propofol is often used in patients who have coronary artery disease undergoing a wide variety of surgical procedures, it is important to evaluate the direct effects of propofol on K(ATP) channel activities in ventricular myocardium during ischemia. The effects of propofol (0.4-60.1 microg/ml) on both sarcolemmal and mitochondrial K(ATP) channel activities were investigated in single, quiescent rat ventricular myocytes. Membrane currents were recorded using cell-attached and inside-out patch clamp configurations. Flavoprotein fluorescence was measured to evaluate mitochondrial oxidation mediated by mitochondrial K(ATP) channels. In the cell-attached configuration, open probability of K(ATP) channels was reduced by propofol in a concentration-dependent manner (EC(50) = 14.2 microg/ml). In the inside-out configurations, propofol inhibited K(ATP) channel activities without changing the single-channel conductance (EC(50) = 11.4 microg/ml). Propofol reduced mitochondrial oxidation in a concentration-dependent manner with an EC(50) of 14.6 microg/ml. Propofol had no effect on the sarcolemmal K(ATP) channel activities in patch clamp configurations and the mitochondrial flavoprotein fluorescence induced by diazoxide at clinically relevant concentrations (< 2 microm), whereas it significantly inhibited both K(ATP) channel activities at very high, nonclinical concentrations (> 5.6 microg/ml; 31 microm).
(キーワード): アデノシン三リン酸 (adenosine triphosphate) / Anesthetics, Intravenous / Animals / Dose-Response Relationship, Drug / 心臓 (heart) / Heart Ventricles / Male / Mitochondria, Heart / Potassium Channels / Propofol / Rats / Rats, Wistar / Sarcolemma
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200206000-00029
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12170062; ● Summary page in Scopus @ Elsevier: 2-s2.0-0036260435

(DOI: 10.1097/00000542-200206000-00029, PubMed: 12170062, Elsevier: Scopus) Hiroko Segawa, Ichiro Kaneko, Akira Takahashi, Masashi Kuwahata, Mikiko Ito, Ohkido Ichiro, Sawako Tatsumi and Ken-ichi Miyamoto :
Growth-related renal Type II Na/Pi cotransporter.,
The Journal of Biological Chemistry, Vol.277, No.22, 19665-19672, 2002.

(キーワード): DEPENDENT PHOSPHATE COTRANSPORTER / BORDER MEMBRANE-VESICLES / AMINO-ACID TRANSPORTER / RAT-KIDNEY / IDENTIFICATION / EXPRESSION / NAPI-2 / REABSORPTION / ONTOGENY / OOCYTES
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M200943200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11880379; ● CiNii @ 国立情報学研究所 (CRID): 1572824501584477568; ● Search Scopus @ Elsevier (PMID): 11880379; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M200943200

(DOI: 10.1074/jbc.M200943200, PubMed: 11880379, CiNii: 1572824501584477568) Akira Takahashi, Wada Akihiro, Ogushi Ken-ichi, Maeda Kyoko, Kawahara Tsukasa, Kazuaki Mawatari, Hisao Kurazono, Moss Joel, Hirayama Toshiya and Yutaka Nakaya :
Production of L-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein,
FEBS Letters, Vol.508, No.3, 484-488, 2001.

(要約): We recently showed that FliC of Salmonella enteritidis increased human beta-defensin-2 (hBD-2) expression, and now describe the signaling responsible pathway. FliC increased the intracellular Ca(2+) concentration ([Ca(2+)](in)) in Caco-2 cells. The [Ca(2+)](in) increase induced by FliC was prevented by U73122 and heparin, but not by chelating extracellular Ca(2+) or pertussis toxin. The FliC-induced increase in hBD-2 promoter activity via nuclear factor kappaB (NF-kappaB) was also inhibited by chelation of intracellular Ca(2+) or by U73122. We conclude that FliC increased [Ca(2+)](in) via inositol 1,4,5-trisphosphate, which was followed by up-regulating hBD-2 mRNA expression via an NF-kappaB-dependent pathway.
(キーワード): Caco-2 Cells / Calcium / Cell Nucleus / Chelating Agents / Colon / Culture Media / Egtazic Acid / Estrenes / Flagellin / Heparin / Humans / Inositol 1,4,5-Trisphosphate / Intestinal Mucosa / NF-kappa B / Pertussis Toxin / Promoter Regions, Genetic / Pyrrolidinones / RNA, Messenger / Salmonella enteritidis / Signal Transduction / Up-Regulation / Virulence Factors, Bordetella / beta-Defensins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0014-5793(01)03088-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11728477; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035941137

(DOI: 10.1016/S0014-5793(01)03088-5, PubMed: 11728477, Elsevier: Scopus) Akira Takahashi, Toshitaka Ikehara, Keiko Hosokawa and Hisao Yamaguchi :
Properties of Ca²⁺-dependent K⁺ channels of human gingival fibroblasts,
Journal of Dental Research, Vol.74, No.8, 1507-1512, 1995. Zhen-Lin Jiang, Hisao Yamaguchi, Hiroyuki Tanaka, Akira Takahashi, Shingo Tanabe, Noboru Utsuyama, Toshitaka Ikehara, Keiko Hosokawa, Yohsuke Kinouchi and Hiroshi Miyamoto :
Blood flow velocity in the commom carotid artery in humans during graded exercise on a treadmill,
Europian Journal of Physiology, Vol.70, 234-239, 1995. Jufang He, Lin Zen Jiang, Hiroyuki Tanaka, Toshitaka Ikehara, Akira Takahashi, Hisao Yamaguchi, Hiroshi Miyamoto, Tadamitsu Iritani and Yohsuke Kinouchi :
Changes in caritid blood flow and electrocardiogram in humans during and after walking on a treadmill,
European Journal of Applied Physiology and Occupational Physiology, Vol.67, No.6, 486-491, 1993.

(要約): Blood flow velocity in the common carotid artery and the electrocardiogram were measured simultaneously by telemetry in seven male subjects during 20-min walking on a treadmill at an exercise intensity corresponding to a mean oxygen uptake of 26.0 (SD 2.9) ml.kg-1.min-1. The mean cardiac cycle was shortened from 0.814 (SD 0.103) s to 0.452 (SD 0.054) s during this exercise. Of this shortening, 73% was due to shortening of the diastolic period and 27% to shortening of the systolic period. In the relatively small shortening of the mean systolic period [from 0.377 (SD 0.043) s to 0.268 (SD 0.029) s], the isovolumetric contraction time was shortened by 56%. During exercise, the heart rate (fc) increased by 79.4% [from 74.3 (SD 9.3) beats.min-1 to 133.3 (SD 14.8) beats.min-1], and the peak blood velocity (S1) in the common carotid artery increased by 56.1% [from 0.82 (SD 0.10) m.s-1 to 1.28 (SD 0.11) m.s-1]. After exercise, the S1 decreased rapidly to the resting level. The fc decreased more slowly, still being higher than the initial resting level 5 min after exercise. The diastolic velocity wave and the end-diastolic foot decreased during exercise. The blood flow rate in the carotid artery increased transiently by 13.5% at the beginning of exercise [from 5.62 (SD 0.63) ml.s-1 to 6.38 (SD 0.85) ml.s-1] and by 26.5% at the end of the exercise period [from 5.62 (SD 0.63) ml.s-1 to 7.11 (SD 1.34) ml.s-1].(ABSTRACT TRUNCATED AT 250 WORDS)
(キーワード): Adult / Blood Pressure / Carotid Artery, Common / Electrocardiography / Exercise / Exercise Test / Heart Rate / Humans / Male / Oxygen Consumption / Regional Blood Flow / Time Factors / Walking
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/BF00241643
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8149926; ● Summary page in Scopus @ Elsevier: 2-s2.0-0027729117

(DOI: 10.1007/BF00241643, PubMed: 8149926, Elsevier: Scopus) Hisao Yamaguchi, Keiko Hosokawa, Zheng-Lin Jiang, Akira Takahashi, Toshitaka Ikehara and Hiroshi Miyamoto :
Arrest of cell cycle progression of HeLa cells in the early G1 phase in K +-depleted conditions abd its recovery upon addition of insulin and LDL,
Journal of Cellular Biochemistry, Vol.53, No.1, 13-20, 1993. Toshitaka Ikehara, Hisao Yamaguchi, Keiko Hosokawa, Akira Takahashi and Hiroshi Miyamoto :
Kinetic study on the effects of intracellular K+ and Na+ on Na+,K+,Cl- cotransport of HeLa cells by Rb+ influx determination,
The Journal of Membrane Biology, Vol.132, 115-124, 1993.

(キーワード): furosemide / HeLa cells / Na;,K+,Cl- cotransport / potassium / rubidium influx

MISC

Hiroki Mori, Yuji Morine, Kazuaki Mawatari, Ayumi Chiba, Shin-ichiro Yamada, Yu Saitou, Hiroki Ishibashi, Akira Takahashi and Mitsuo Shimada :
Bile Metabolites and Risk of Carcinogenesis in Patients With Pancreaticobiliary Maljunction: A Pilot Study.,
Anticancer Research, Vol.41, No.1, 327-334, 2021.

(要約): Pancreaticobiliary maljunction (PBM), a disease with reflux of pancreatic and bile juice in the pancreaticobiliary tract, is a high-risk factor for biliary tract cancer. The aim of this study was to investigate the mechanism of carcinogenesis in PBM using a metabolomics analysis of bile sampled during surgery. Three patients with PBM without biliary tract cancer, four patients with extrahepatic bile duct cancer (EHBC), and three controls with benign disease were enrolled. Metabolomics analysis of bile samples was performed using capillary electrophoresis-mass spectrometry and liquid chromatography-mass spectrometry to discriminate the amino acid and lipidomic profiles. The principal component analysis in the capillary electrophoresis-mass spectrometry and liquid chromatography-mass spectrometry revealed similar metabolites in patients with PBM and those with EHBC; furthermore, there was a clear difference between patients with PBM or EHBC compared to controls. The amino acid profiles revealed the following 20 potential carcinogenic candidates for PBM: isoleucine, phenylalanine, tyrosine, leucine, tryptophan, arginine, lysine, valine, asparagine, methionine, aspartic acid, serine, threonine, histidine, glutamine, alanine, proline, glutamic acid, and pyruvic acid. The lipidomic profiles revealed the following 11 carcinogenic candidates: lysophosphatidylcholine, lysophosphatidylethanolamine, phosphatidyl glycerol, lysophosphatidyl glycerol, triacylglycerol, diacylglycerol, ceramide, sphyngomyeline, fatty acid, hyperforin, and vitamin D. Among these characteristic metabolites, the branched-chain amino acids, methionine and lysophosphatidylcholine are known to be related to carcinogenesis. The bile metabolites were extremely similar in patients with PBM and those with EHBC. Furthermore, amino acid and lipid metabolism was markedly different in patients with PBM or EHBC compared to healthy controls.
(キーワード): Bile / Bile Duct Neoplasms / Cell Transformation, Neoplastic / Chromatography, Liquid / Disease Susceptibility / Electrophoresis, Capillary / Female / Humans / Male / Mass Spectrometry / Metabolomics / Pancreaticobiliary Maljunction / Pilot Projects / Risk Assessment / Risk Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.21873/anticanres.14779
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33419827; ● Search Scopus @ Elsevier (PMID): 33419827; ● Search Scopus @ Elsevier (DOI): 10.21873/anticanres.14779

(DOI: 10.21873/anticanres.14779, PubMed: 33419827) Noriko Matsumoto, Kouzou Yoshikawa, Mitsuo Shimada, Nobuhiro Kurita, Horohiko Sato, Takashi Iwata, Jun Higashijima, Motoya Chikakiyo, Masaaki Nishi, Hideya Kashihara, Chie Takasu, Shohei Eto, Akira Takahashi, Masatake Akutagawa and Takahiro Emoto :
Effect of light irradiation by light emitting diode on colon cancer cells.,
Anticancer Research, Vol.34, No.9, 4709-4716, 2014.

(要約): Recent studies have demonstrated the efficacy of irradiation from light emitting diodes (LED) for wound healing, anti-inflammation and anticancer therapies. However, little is known about the effects of visible light in colon cancer cells. The purpose of this study was to evaluate the biological response (including gene expression changes) of human colon cancer cells to different wavelengths of LED irradiation. Human colon cancer cells (HT29 or HCT116) were seeded onto laboratory dishes that were then put on LED irradiation equipment with a 465 nm-, 525 nm-, or 635 nm-LED. Irradiation at 15 or 30 mW was performed 10 min/day, each day for 5 days. The cell counting kit8 was then used to measure cell viability. Apoptosis and expression of several mRNAs (caspase, MAPK and autophagy pathway) in HT29 cultures irradiated with 465 nm LED were evaluated via AnnexinV/PI and RT-PCR, respectively. Viability of HT29 and HCT116 cells was lower in 465 nm-LED irradiated cultures than in control cultures, but viability of HT29 cells did not differ between control cultures and 525 nm-LED or 635 nm-LED irradiated cultures. Moreover, the expression of FAS, caspase-3, capase-8, and JUK were significantly higher in 465 nm-LED irradiated cultures than in control cultures, and expression of ERK1/2 and LC3 was lower in blue-irradiated cells. LED irradiation at 465 nm inhibited the proliferation of HT29 cells and of HCT116 cells. Notably, LED irradiation at 465 nm promoted apoptosis inHT29 cultures via the extrinsic apoptosis pathway and the MAPK pathway.
(キーワード): アポトーシス (apoptosis) / Cell Cycle Checkpoints / Cell Line, Tumor / Cell Proliferation / Cell Survival / Colonic Neoplasms / Dose-Response Relationship, Radiation / Gene Expression Regulation, Neoplastic / HCT116 Cells / HT29 Cells / Humans / Lasers, Semiconductor / Light / Mitogen-Activated Protein Kinases / シグナル伝達 (signal transduction)
(徳島大学機関リポジトリ): ● Metadata: 109346
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25202048; ● Summary page in Scopus @ Elsevier: 2-s2.0-84908691381

(徳島大学機関リポジトリ: 109346, PubMed: 25202048, Elsevier: Scopus) Rie Matsushima, Akira Takahashi, Yutaka Nakaya, Mari Miki, Yoichi Nakamura, Fumiko Michishige, Sumiko Yoshinaga, Hiroshi Maezawa and Susumu Yasuoka :
Tripsin-like activities and human airway trypsin-like protease (HAT) levels in airway secretions from healthy subjects and patients with chronic airway diseases.,
Proceeding of Airway Secretion Research, Vol.7, 15-22, 2005.

(キーワード): Trypsin-like protease / HAT / airway secretions / chronic airway diseases

総説・解説

篠田浩一, 馬渡一諭, 髙橋章 :
家庭の安全・安心科学 ―家庭における微生物汚染とその対策―, --- 紫外線を使った身の回りにある電化製品とその利用の注意点 ---,
日本防菌防黴学会誌, Vol.52, No.2, 51-56, 2024年. 中橋睦美, 髙橋章 :
紫外線LEDの医療への応用,
小児科, Vol.57, No.8, 1011-1016, 2016年7月. 中橋睦美, 髙橋章 :
紫外線LEDを用いた殺菌システムと応用 (特集先進的環境プロセス技術の展開),
ケミカルエンジニヤリング = Chemical engineering, Vol.61, No.6, 428-432, 2016年6月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520010380144585472

(CiNii: 1520010380144585472) 髙橋章, 粟飯原睦美, 馬渡一諭, 芥川正武 :
近紫外線発光ダイオードを用いた殺菌システム,
食品分野における非加熱殺菌技術, 150-166, 2013年11月. 粟飯原睦美, 髙橋章 :
近紫外線発光ダイオードを用いた食肉表面殺菌法の開発,
養牛の友, No.445, 76-79, 2013年4月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522543654692705408

(CiNii: 1522543654692705408) 常冨愛香里, 粟飯原睦美, 髙橋章 :
新UVA-LEDの環境浄化への応用と展開 (特集紫外線利用による環境浄化の現状と課題),
空気清浄 : コンタミネーションコントロール, Vol.51, No.3, 33-36, 2013年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520854805035201792

(CiNii: 1520854805035201792) 粟飯原睦美, 髙橋章 :
食品製造分野におけるLED殺菌技術の可能性 (特集洗浄・殺菌技術の近況),
ジャパンフ-ドサイエンス, Vol.48, No.6, 20-26, 2009年6月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522543655595785856

(CiNii: 1522543655595785856) 中屋豊, 馬渡一諭, 原田永勝, 髙橋章 :
侵襲時の栄養評価法,
機能性食品と薬理栄養, Vol.4, No.4, 215-218, 2007年9月. 中屋豊, 原田永勝, 馬渡一諭, 髙橋章, 保坂利男 :
メタボリック症候群における高血圧の管理,
四国医学雑誌, Vol.63, No.3,4, 97-100, 2007年8月.

(要約): Metabolic syndrome includes abdominal obesity, hyperlipidemia, diabetes, and hypertension. All, but hypertension, are obviously related to metabolism. However, hypertension might result from, at least in part, abdominal obesity, because adipose tissue produces bioactive mediators (adipocytokines)which increase blood pressure. In treatment of hypertension, we should concern insulin resistance, which is a major risk factor of cardiovascular events. Angiotensin converting enzyme inhibitor is known to improve insulin resistance, but results of angiotensin receptor blocker in animal studies are controversial. In clinical trial, there are many established data that ARBs prevent new onset of diabetes mellitus, suggesting that this agent also has a beneficial effect on glucose metabolism. Short acting Ca-antagonists, such as nifedipine, decrease insulin sensitivity, but long-acting Ca-antagonists increase it. βblockers decrease insulin sensitivity but those with α-blocking action improve insulin resistance. Recent study, ARB is more potent to reduce cardiovascular risk in those with obesity than in those with normal body weight, suggesting some drugs are more effective in metabolic syndrome. Thus, when we chose antihypertensive drugs in treating patients with metabolic syndrome, we have to choose proper drugs in addition to modify life-style.
(キーワード): hypertension / diabetes mellitus / metabolic syndrome / angiotensin / insulin resistance
(徳島大学機関リポジトリ): ● Metadata: 110178
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050001337465066624

(徳島大学機関リポジトリ: 110178, CiNii: 1050001337465066624) Yutaka Nakaya, Kazuaki Mawatari, Akira Takahashi, Nagakatsu Harada, Hata Akiko and Yasui Sonoko :
The phytoestrogen ginsensoside Re activates potassium channels of vascular smooth muscle cells through PI3K/Akt and nitric oxide pathways,
The Journal of Medical Investigation : JMI, Vol.54, No.3,4, 381-384, Aug. 2007.

(要約): In vascular smooth muscle cells, large-conductance Ca(2+)-activated K(+) channels (K(Ca) channels) play a pivotal role in determining membrane potential, and thereby the vascular tone. Ginsenoside Re, a phytochemical from ginseng, is reported to activate this channel, but its precise mechanism is unsolved. Patch clamp studies showed that ginsenoside Re activates K(Ca) channels in the arterial smooth muscle cell line A10 in a dose-dependent manner. The channel-opening effect of ginsenoside Re was inhibited by 1 microM L-NIO, an inhibitor of eNOS, but not by 3 microM SMTC, an inhibitor of nNOS, indicating that ginsenoside Re activated K(Ca) channels through activation of eNOS. SH-6 (10 microM), an Akt inhibitor, and wortmannin, a PI3-kinase inhibitor, completely blocked activation of K(Ca) channels by ginsenoside Re, indicating that it activates eNOS via a c-Src/PI3-kinase/Akt-dependent mechanism. In addition, the ginsenoside Re-induced activation of eNOS and K(Ca) channel was blocked by 10 microM ICI 182, 780, an inhibitor of membrane estrogen receptor-alpha, suggesting that eNOS activation occurs via a non-genomic pathway of this receptor. In conclusion, ginsenoside Re releases NO via a membrane sex steroid receptors, resulting in K(Ca) channel activation in vascular smooth muscle cells, promoting vasodilation and preventing severe arterial contraction.
(キーワード): Animals / Cell Line / Ginsenosides / Muscle, Smooth, Vascular / Myocytes, Smooth Muscle / Nitric Oxide / Nitric Oxide Synthase Type III / Phosphatidylinositol 3-Kinases / Phytoestrogens / Potassium Channels, Calcium-Activated / Proto-Oncogene Proteins c-akt / Rats / Vasodilation
(徳島大学機関リポジトリ): ● Metadata: 111546
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.54.381
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17878692; ● Search Scopus @ Elsevier (PMID): 17878692; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.54.381

(徳島大学機関リポジトリ: 111546, DOI: 10.2152/jmi.54.381, PubMed: 17878692) 北村清一郎, 篠原千尋, 髙橋章 :
見える!わかる!臨床に役立つ解剖写真集—7, --- 顎関節の局所解剖学—顎関節症を理解するために— ---,
歯界展望, Vol.108, No.1, 72-78, 2006年.

講演・発表

Takeshi Nikawa, Katsuyuki Miyawaki, Akira Takahashi, Takahito Watanabe and Taro Mito :
ANTI-MUSCLE ATROPHIC PROTEIN FOOD SOURCE IN SPACE: DEVELOPMENT OF A RECIRCULATORY REARING SYSTEM FOR SOYBEANS AND CRICKETS,
45th COSPAR Scientific Assembly-COSPAR 2024, Jul. 2024. Kai Ishida, Takaaki Shimohata, Kanda Yuna, Nguyen Quoc Anh, Masuda Rumiko, Yamazaki Kohei, Takashi Uebanso, Kazuaki Mawatari, Kashimoto Takashige and Akira Takahashi :
Characteristic metabolic changes in the infected tissue due to Vibrio vulnificus in a wound infection model.,
57th United States Japan Cooperative Medical Science Program Joint Panel Conference on Cholera and Other Bacterial Enteric Infections, Dec. 2023. SHINODA Koichi, Kazuaki Mawatari, Bui K. N. T., Hirakawa H., Awamoto K., Wakitani M, Shinoda T. and Akira Takahashi :
Development of novel far-UVC light source for high germicidal effects and low human health risk.,
2023 IEEE Photonics Conference, Orlando, Nov. 2023. Akira Takahashi, Katsuyuki Miyawaki, Kazuaki Mawatari, Takeshi Nikawa, Mutsumi Aihara, Fukushima Shiho, Akizawa Shinta, Yamashita Michiyo and Koi Yumena :
Development of closed-circulation soybean cultivation system applicable to extreme environments,
The 3rd Japan-France International Symposium on Space Nutrition/Medicine, Kyoto, Nov. 2023. Shintaro Inoue, Takahito Watanabe, Hamaguchi Taiki, Fujie Kai, Shimamura Ayane, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji and Taro Mito :
Artificial modification of cricket body color: breeding for the next-generation of protein supply,
International Conference of Non-Traditional Arthropod Model Systems, Aug. 2023. Fujie Kai, Shintaro Inoue, Hamaguchi Taiki, Yoshiyasu Ishimaru, Katsuyuki Miyawaki, Takeshi Nikawa, Akira Takahashi, Sumihare Noji, Takahito Watanabe and Taro Mito :
The discovery of two paralogous dopamine-synthase genes in the two-spotted cricket Gryllus bimaculatus,
International Conference of Non-Traditional Arthropod Model Systems, Aug. 2023. Onoda Yushi, Kai Ishida, Kadomura-Ishikawa Yasuko, Nagahashi Miharu, Yamashita Michiyo, Fukushima Shiho, Aizawa Toshihiko, Yamauchi Shigeharu, Fujikawa Yasuo, Tanaka Tomotake, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Investigation of the wavelength dependence of fungal inactivation by standardized UV-LED irradiation device,
The 10th Congress of European Microbiologists FEMS 2023, Hamburg, Jul. 2023. Kai Ishida, Takaaki Shimohata, Kanda Yuna, Nguyen Quoc Anh, Masuda Rumiko, Yamazaki Kohei, Takashi Uebanso, Kazuaki Mawatari, Kashimoto Takashige and Akira Takahashi :
Characteristic metabolic changes in the infected tissue due to Vibrio vulnificus in a wound infection model,
日米コレラ部会(日米医学協力研究会コレラ・細菌性腸管感染症専門部会), Jul. 2023. SHINODA Koichi, NGAN THI KIM BUI, Kazuaki Mawatari, HIRAKAWA H., AWAMOTO K., Wakitani M., SHINODA T. and Akira Takahashi :
Application Of Far-UVC Radiation For Sterilization Using New Strategies To Reduce Human Health Risks And Environmental Loads,
ASM Microbe 2023, Houston, Jun. 2023. Kai Ishida, Onoda Yushi, Yasuko Ishikawa, Nagahashi Miharu, Yamashita Michiyo, Fukushima S., Aizawa T., Yamauchi S., Fujikawa Y., Tanaka T., Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Evaluation of wavelength dependent inactivation of bacteria, viruses, and fungi by originally developed light source with UV-LEDs,
ASM Microbe 2023, Houston, Jun. 2023. NGAN THI KIM BUI, SHINODA Koichi, Kazuaki Mawatari, HIRAKAWA H., AWAMOTO K., Wakitani M., SHINODA T. and Akira Takahashi :
Determination of Optimum Wavelength of Far-UVC for Virucidal and Bactericidal Effects Using Plasma Emission-Based Light Modules,
ASM Microbe 2023, Houston, Jun. 2023. Hitoshi Takagi, Antonio Norio Nakagaito, Nozomi Kawakami, Akira Takahashi and Takeshi Nikawa :
Isolation of cellulose nanofibers from soybean waste,
The 9th International Forum on Advanced Technologies and The 4th Japan-Taiwan International Engineering Forum (IFAT & JTIEF 2023), 50033_1-50033_2, Taipei, Mar. 2023. Shiho Fukushima, Takaaki Shimohata, Junko Kido, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Campylobacter jejuni induce autophagy for the bacterial invasion in the host epithelial cells,
54th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Dec. 2019. Toshitaka Ikehara, Mutsumi Nakahashi, Takahiro Emoto, Masatake Akutagawa, Koichiro Tsuchiya, Akira Takahashi and Yohsuke Kinouchi :
Effects of reactive oxygen species induced by 405 nm light irradiation on Hela S3 cells,
The Joint Annual Meeting of the Bioelectromagnetics Society and the European BioElectromagnetics Association, Montpellier, Jun. 2019. Aya Tentaku, Takaaki Shimohata, Sho Hatayama, Junko Kido, Anh Quoc Nguyen, Yuna Kanda, Shiho Fukushima, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Role of host cellular unfolded protein response signaling during Campylobacter jejuni infection,
53th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Mar. 2019. Anh Quoc Nguyen, Takaaki Shimohata, Sho Hatayama, Aya Tentaku, Junko Kido, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Metabolic changing in epithelial cell during Vibrio parahaemolyticus infection,
53th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Mar. 2019. Yukiko Tomioka, Hidehiro Umehara, Shinya Watanabe, Masahito Nakataki, Masuda Rumiko, Kazuaki Mawatari, Takeshi Nikawa, Akira Takahashi, Shusuke Numata and Tetsuro Ohmori :
Altered plasma metabolites related to one-carbon metabolism in schizophrenia.,
WFSBP Asia Pacific Regional Congress of Biological Psychiatry, Kobe, Sep. 2018. Toshiya Okahisa, Masahiro Sogabe, Mayu Uyama, Tadahiko Nakagawa, Tetsu Tomonari, Tatsuya Taniguchi, Koichi Okamoto, Tetsuji Takayama, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi, Takahiro Emoto, Masatake Akutagawa, M Yamada and M Fukuhara :
Development Of A Multi-Ring Type Roller Pump Unit Equipped To A Compact And Convenient Ascites Purification Machine For Cell-Free And Concentrated Ascites Reinfusion Therapy (CART).,
ASAIO 64th Annual Conference, Washington, D.C., Jun. 2018. Aya Tentaku, Takaaki Shimohata, Sho Hatayama, Anh Quoc Nguyen, Junko Kido, Shiho Fukushima, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Induction of the unfolded protein response (UPR) decreased Campylobacter jejunin invasion,
118th General Meeting of the American Society for Microbiology, Jun. 2018. Shiho Fukushima, Yuri Sato, Takaaki Shimohata, Junko Kido, Yuna Kanda, Aya Tentaku, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Campylobacter jejuni Utilized Autophagy for the Bacterial Survival in the Host Epithelial Cells,
118th General Meeting of the American Society for Microbiology, Jun. 2018. Junko Kido, Takaaki Shimohata, sachie amano, sho hatayama, yuri sato, yuna kanda, aya tentaku, shiho Fukushima, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
CFTR reduced microtubule-mediated Campylobacter jejuni invasion,
52th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Feb. 2018. Syo Hatayama, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
The relation of cellular Tight Junctions formation and Campylobacter jejuni invasion in intestinal epithelial cells,
51th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Feb. 2017. Asami Iwanaka, Mamiko Soga, Eiichi Kotake-Nara, Akihito Nagao, Rie Mukai, Akira Takahashi and Junji Terao :
Effect of Fucoxanthin and Neoxanthin on Blue Light-Emitting Diode-Irradiated Photosensitized Oxidation in Liposomal Membranes andMouse Fibroblast Cells,
The 6th International Conference on Food Factors: Bioconvergence for Food Function, Soul, Republic of Korea, Nov. 2015. Tadahiko Nakagawa, Yoshifumi Takaoka, Hironori Tanaka, Kumiko Tanaka, Tetsuji Takayama, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi, Masatake Akutagawa, Takahiro Emoto, Yohsuke Kinouchi, Toru Murashima, Satoru Yamaji, Zenji Tanaka, Masahiro Sogabe and Toshiya Okahisa :
Roller Pump Circulation System For Preventing Filter Clogging During Cell-free and Concentrated Ascites Reinfusion Therapy (cart).,
The 61th Annual Conference of American Society for Artificial Internal Organs(ASAIO), Chicago, Jun. 2015. Tsunedomi Akari, Masamura Akinori, Mutsumi Nakahashi, Nishisaka Risa, Kazuaki Mawatari, Takaaki Shimohata, Takashi Uebanso, Katsuyuki Miyawaki, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Disinfection system with UVA-LED for hydroponic nutrient solution,
6th FEMS Microbiology Congress, Jun. 2015. Hatayama Sho, Takaaki Shimohata, Negoro Sachie, Sato Yuri, Kido Junko, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
The denudation of lateral part promote Campylobacter jejuni invasion in Caco-2 cells.,
6th FEMS Microbiology Congress, Jun. 2015. Takashi Uebanso, Takaaki Shimohata, Iba Hitomi, Nishimura Kazuya, Taniguchi Yuichi, Kazuki Horikawa, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi :
COMBINATION BETWEEN A FEW T3SS INJECTISOME AND A LOT EFFECTOR FOR KILLING HOST CELLS ON VIBRIO PARAHAEMOLYTICUS,
6th FEMS Microbiology Congress, Jun. 2015. Takaaki Shimohata, Negoro Sachie, Kido Junko, Hatayama Sho, Sato Yuri, Iba Hitomi, Mutsumi Nakahashi, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
INFECTION OF CAMPYLOBACTER JEJUNI REDUCES CFTR MEDIATED CL- SECRETION IN T-84,
6th FEMS Microbiology Congress, Jun. 2015. Ayumi Yoshimoto, Takashi Uebanso, Kazuaki Mawatari, Mutsumi Nakahashi, Takaaki Shimohata and Akira Takahashi :
Low dose antibiotics treatment in the prenatal period affect newborns health in later life,
12th Asian Congress of Nutrition, 70, May 2015. Takashi Uebanso, Ai Ohnishi, Takaaki Shimohata, Mutsumi Nakahashi, Kazuaki Mawatari and Akira Takahashi :
Acceptable daily intake levels of sucralose consumption reduces fecal Clostridium cluster 4 in mouse,
12th Asian Congress of Nutrition, 70, May 2015. Takaaki Shimohata, Sachie Negoro, Sho Hatayama, Yuri Sato, Hitomi Iba, Mutsumi Aihara, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Campylobacter jejuni Infection Reduces Cftr Mediated Cl- Secretion In T-84,
American Society for Microbiology 114th General Meeting, May 2014. Mutsumi Aihara, Takashi Uebanso, Takaaki Shimohata, Kazuaki Mawatari, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on Vibrio parahaemolyticus,
48th US-Japan Cooperative Medical Sciences Program Conference on cholera and Other Bacterial Enteric Infections, Feb. 2014. Sachie Negoro, Takaaki Shimohata, Syo Hatayama, Mari Matsumoto, Yuri Sato, Mutsumi Aihara, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Suppression of CFTR-mediated Cl- transport in Campylobacter jejuni infection,
International Symposium Hannover - Tokushima Research Communication, Aug. 2013. Takashi Uebanso, Hitomi Iba, Shoko Asada, Takaaki Shimohata, Mutsumi Aihara, Kazuaki Mawatari and Akira Takahashi :
Regulation and effects of VP1671 (VscQ) on virulence of Vibrio parahaemolyticus,
5th FEMS Microbiology Congress, Jul. 2013. Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Synergistic bactericidal effect of ultraviolet light with a combination of different wavelength,
5th FEMS Microbiology Congress, Jul. 2013. Kazuaki Mawatari, Tam Thanh Thi Le, Miki Maetani, Tomomi Yamamoto, Airi Honjo, Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi :
VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress,
5th FEMS Microbiology Congress, Jul. 2013. Teppei Hoshiyama, Masatake Akutagawa, Mutsumi Aihara, Mario Hayashida, Akira Takahashi, Takahiro Emoto, Shinsuke Konaka and Yohsuke Kinouchi :
Gene expression analysis of Vibrio parahaemolyticus for UV irradiation,
BioEM2013, 94, Thessaloniki, Jun. 2013.

(要約): 腸炎ビブリオを紫外線に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．UV-A，UV-B，UV-Cそれぞれの代表的な波長について，腸炎ビブリオにUV照射した後に解析を行い，各波長ごとの遺伝子発現の違いについて調査した．

Kazuaki Mawatari, Tam Thanh Thi Le, Miki Maetani, Tomomi Yamamoto, Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi :
VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress,
US-Japan Cooperative Medical Science Program, 47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections, Dec. 2012. Takashi Uebanso, Hitomi Iba, Shoko Asada, Takaaki Shimohata and Akira Takahashi :
REGULATION AND EFFECTS OF VP1671(VSCQ) ON VIRULENCE Of VIBRIO PARAHAEMOLYTICUS,
International Symposium on Single cell Analysis, Kyoto, Nov. 2012. Masachika Ishizaki, Yusuke Manabe, Mutsumi Aihara, Akira Takahashi, Masatake Akutagawa, Takahiro Emoto, Yohsuke Kinouchi and Toshitaka Ikehara :
Improvement of a pipe type UVA-LED sterilizer using a condenser lens,
34th Annual Conference of the bioelectromagnetics society (34 BEMS) (Abstract), 189-190, Brisbane, Jun. 2012.

(要約): UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた．

Toshitaka Ikehara, Mutsumi Aihara, Zehong Su, Akira Takahashi, Masatake Akutagawa and Yohsuke Kinouchi :
Effects of UVA radiation on growth of RAW 264.7 cells,
34th Annual Conference of the bioelectromagnetics society (34 BEMS) (Abstract), 179-180, Brisbane, Jun. 2012.

(要約): マウス由来のRAW 264.7細胞に対してUVAを照射した際の増殖の違いについて検討した．

Kazuaki Mawatari, Yuka Isumi, Minami Hirayama, Miki Maetani, Tomomi Yamamoto, Sayaka Hayashida, Hitomi Iba, Mutsumi Aihara, Takaaki Shimohata and Akira Takahashi :
OtnA-OtnB modulate capsular polysaccharide and polymyxin B resistance in Vibrio parahaemolyticus,
112th General Meeting of the American Society for Microbiology, Jun. 2012. Mutsumi Aihara, Kazuaki Mawatari, Takaaki Shimohata and Akira Takahashi :
Bactericidal Effect of Ultraviolet Light with a Combination of Different Wavelengths,
112th General Meeting of the American Society for Microbiology, Jun. 2012. Hitomi Iba, Takaaki Shimohata, Masayuki Yamato, Kazuaki Mawatari and Akira Takahashi :
The effect of VP1671 (vscQ) on virulence of Vibrio parahaemolyticus,
4th FEMS Microbiology Congress, Jun. 2011. Kazuaki Mawatari, Yumi Yoneda, Manami Honda, Hitomi Iba, Mutsumi Aihara, Zehong Su, Takaaki Shimohata, Masayuki Yamato and Akira Takahashi :
VPA1604, a protein containing phosphorylated tyrosine, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus,
4th FEMS Microbiology Congress, Jun. 2011. Yuya Manabe, Ryosuke Nakagawa, Su Zhehong, Miki Maetani, Kenji Teranishi, Naoyuki Shimomura and Akira Takahashi :
Influences of Pulsed Electric Fields on the Gene Expression of Pathogenic Bacteria,
Proceedings of the 18th IEEE International Pulsed Power Conference, 1242-1246, Chicago, Jun. 2011.

(出版サイトへのリンク): ● Publication site (DOI): 10.1109/PPC.2011.6191592
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1109/PPC.2011.6191592

(DOI: 10.1109/PPC.2011.6191592) Kazuaki Mawatari, Yumi Yoneda, Manami Honda, Hitomi Iba, Mutsumi Aihara, Zehong Su, Takaaki Shimohata, Masayuki Yamato and Akira Takahashi :
VPA1604, a protein tyrosine kinase, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus,
US-Japan Cooperative Medical Science Program, 45th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Panel, Kyoto, Dec. 2010. Miku Maeda, Yohsuke Kinouchi, Akira Takahashi, Takahiro Emoto, Masatake Akutagawa and Xin Lian :
Novel sterilization device using 265nm UV-LED for escherichia coli,
Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting, 43, Seoul, Jun. 2010. Yuhsuke Manabe, Takahiro Emoto, Masatake Akutagawa, Akira Takahashi and Yohsuke Kinouchi :
Simulation of UVA-LED irradiance distribution,
Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting, 43, Seoul, Jun. 2010. Tsuyosi Okahisa, Masayuki Yamato, Akira Takahashi, Masatake Akutagawa, Takahiro Emoto and Yohsuke Kinouchi :
UVA-LED for sterilizing water in a water tank,
Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting, 42, Seoul, Jun. 2010. Tomohiro Oshita, Keiko Shintani, Mai Katayama, Takahiro Emoto, Masatake Akutagawa, Akira Takahashi and Yohsuke Kinouchi :
An investigation of the sterilization effect of 385nm UVA-LED,
Proceedings of The Bioelectromagnetics Society 32nd Annual Meeting, 43, Seoul, Jun. 2010. Takaaki Shimohata, Masayuki Nakano, Lian Xin, Kazuaki Mawatari, Masayuki Yamato and Akira Takahashi :
Vibrio parahaemolyticus modulate IL-8 secretion through dual pathway,
110th General Meeting of the American Society for Microbiology, San Diego, CA, U.S.A., Jun. 2010. Ryosuke Nakagawa, Kazuki Siroyama, Su Zehong, Kenji Teranishi, Naoyuki Shimomura and Akira Takahashi :
Effects of Pulse electric Fields to the Pathogenic Bacteria,
Proceedings of the 2010 IEEE Power Modulators and High-Voltage Conference, 686-689, Atlanta, May 2010.

(要約): The pulsed power technology is gaining ground on applications to biological field such as sterilization with pulse electric fields or discharges and induction of the apoptosis with pulse electric fields. Under the circumstances, the pulse electric fields clearly have some influence on organism. To figure out the influence and mechanism, we conducted an experiment. The pulse electric fields were applied to the pathogenic bacteria, and manifestation of RNAs which affected pathogenicity was examined. A system was developed to apply the pulsed electric fields to bacteria. The pulsed power generator, which consists of a Blumlein-type pulse forming network (B-PFN), outputs pulses with width of 100 ns in the case of 5 L-C stages. The electrodes chamber was filled with the solution (2 ml) with bacteria. The separation of the electrodes was 5 mm and approximately uniform electric field was applied to the solution. The manifestation of RNAs was analyzed with the method of polymerase chain reaction, PCR Vibrio parahaemolyticus (RIMD 2210633) was selected to examine. The manifestations of RNAs as VP1699, VP1680, VPA1321, VPA1327, VPA1346, TDH, and RNA16S were examined with the PCR method. Although RNA16S does not become an index for pathogenicity, evaluation of RNA16S implies a survival ratio of the bacteria. The initial density of bacteria solution was regulated to be 109 /ml. The peak voltage was approximately 6, 12 kV and the number of applied pulses was 1000 for each sample. The tendency of increase in manifestation was found by the application of pulse electric fields for VP1699 and VP1680 with relation of TTSS(1).
(出版サイトへのリンク): ● Publication site (DOI): 10.1109/IPMHVC.2010.5958451
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1109/IPMHVC.2010.5958451

(DOI: 10.1109/IPMHVC.2010.5958451) Yagi Noriyuki, Mirei Mori, Hamamoto Akiko, Lian Xin, Nakano Masayuki, Masatake Akutagawa, Tachibana Satoko, Akira Takahashi, Toshitaka Ikehara and Yohsuke Kinouchi :
EFFECTS OF 365NM UVA-LED ON BACTERIA,
The Bioelectromagnetics Society 30th Annual Meeting, 350-351, San Diego, Jun. 2008. Akira Takahashi, M. Nakano, Kazuaki Mawatari, Nagakatsu Harada and Yutaka Nakaya :
Vibrio cholerae El tor Hemolysin Activates Cyclic AMT Dependent Cl-Secretory Pathways which Xaused Diarrhea,
108th General Meeting of the American Society for Microbiology, Boston, Jun. 2008. Hamamoto Akiko, Akira Takahashi, Nakano Masayuki, Lian Xin, Tachibana Satoko, Yagi Noriyuki, Tetutani Kayo, Masayuki Yamato, Masatake Akutagawa, Toshitaka Ikehara, Yutaka Nakaya and Yohsuke Kinouchi :
Availability of UVA-light emitting diodes for disinfection system,
第22回生体・生理工学シンポジウム, Harbin, Jan. 2008. Lian Xin, Akira Takahashi, Maeda Miku, Yagi Noriyuki, Tachibana Satoko, Masayuki Yamato, Hamamoto Akiko, Masatake Akutagawa, Nakano Masayuki, Yutaka Nakaya and Yohsuke Kinouchi :
New surface sterilization system using UV-LED for vegetables,
Proceedings of the International Symposium on Biological and Physiological Engineering /The 22nd SICE Symposium on Biological and Physiological Engineering, 257-259, Harbin, Jan. 2008.

(キーワード): UV-LED / surface / sterilization / 365nm

Hamamoto Akiko, Akira Takahashi, Nakano Masayuki, Lian Xin, Tachibana Satoko, Yagi Noriyuki, Tetutani Kayo, Tetsuro Koga, Masayuki Yamato, Masatake Akutagawa, Toshitaka Ikehara and Yohsuke Kinouchi :
Availability of UVA-light emitting diodes for disinfection system,
Proceedings of the International Symposium on Biological and Physiological Engineering /The 22nd SICE Symposium on Biological and Physiological Engineering, 109-112, Harbin, Jan. 2008. Yagi Noriyuki, Mirei Mori, Hamamoto Akiko, Nakano Masayuki, Masatake Akutagawa, Tachibana Satoko, Akira Takahashi, Toshitaka Ikehara and Yohsuke Kinouchi :
Development of a tank-type sterilization device using 365 nm UVA-LED,
Proceedings of the International Symposium on Biological and Physiological Engineering /The 22nd SICE Symposium on Biological and Physiological Engineering, 113-116, Harbin, Jan. 2008. Lian Xin, Akira Takahashi, Nakano Masayuki and Yutaka Nakaya :
Vibrio parahaemolyticus elevates interferon alpha production in intestinal-like epithelial cells CACO-2 cells,
VIBRIO 2007(The second conference on the Biology of Vibrios), Paris, Nov. 2007. Nakano Masayuki, Akira Takahashi and Yutaka Nakaya :
Adrenergic regulation of Vibrio parahaemolyticus pathogenicity by the modulation of viburence-associated gene expressions,
VIBRIO 2007(The second conference on the Biology of Vibrios), Paris, Nov. 2007. Noriyuki Yagi, Mirei Mori, Akiko Hamamoto, Masatake Akutagawa, Satoko Tachibana, Akira Takahashi, Toshitaka Ikehara, Yohsuke Kinouchi and Masayuki Nakano :
Sterilization Using 365 nm UV-LED.,
29th IEEE EMBS Annual International Conference, 5841-5844, Lyon, France, Aug. 2007.

(出版サイトへのリンク): ● Publication site (DOI): 10.1109/IEMBS.2007.4353676
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-57649207950

(DOI: 10.1109/IEMBS.2007.4353676, Elsevier: Scopus) Hamamoto Akiko, Mori Mirei, Nakano Masayuki, Wakikawa Noriko, Toshitaka Ikehara, Yohsuke Kinouchi, Masatake Akutagawa, Akira Takahashi and Yutaka Nakaya :
Water disinfection system using UVA-light emitting diodes and its effects on bacterial DNA.,
107th ASM general meeting, Toronto,Canada, May 2007. Akira Takahashi, Nakano Masayuki, Miyoshi Shinichi, Hamamoto Akiko and Yutaka Nakaya :
Pore formation of Vibrio mimicus hemolysin in lipid bilayers.,
107th ASM general meeting, Toronto, Canada, May 2007. Akira Takahashi, Hamamoto Akiko, Wakikawa Noriko, Mori Mirei, Nakano Masayuki, Masatake Akutagawa, Yohsuke Kinouchi and Yutaka Nakaya :
New water sterilization system used by UVA-LED.,
The 41st Joint Conference US-Japan Cooperative Medical Science Program Cholera Panel, Gifu, Japan, Nov. 2006. Nakano Masayuki, Akira Takahashi and Yutaka Nakaya :
Norepinephrine modulates the pathogencity of Vibrio parahaemolyticus.,
The 41st Joint Conference US-Japan Cooperative Medical Science Program Cholera Panel, Gifu, Japan, Nov. 2006. Morishima Masaki, Akira Takahashi and Yutaka Nakaya :
Transthyretin and serotonin levels in the hippocampus are decreased in SPORTS rats.,
36th Society for Neuroscience, Atlanta, USA, Oct. 2006. Mirei Mori, Akiko Hamamoto, Masayuki Nakano, Masatake Akutagawa, Akira Takahashi, Toshitaka Ikehara and Yohsuke Kinouchi :
Effect of ultraviolet LED on bacteria,
Proceedings of 2006 World Congress on Medical Physics and Biomedical Engineering, Seoul, Korea, August 27-September 1, 2006, Vol.1, 1218-1221, Seoul, Aug. 2006. Mirei Mori, Hamamoto Akiko, Nakano Masayuki, Masatake Akutagawa, Akira Takahashi, Toshitaka Ikehara and Yohsuke Kinouchi :
Effects of ultraviolet LED on bacteria.,
World Congress on Medical Physics and Biomedical Engineering 2006, Korea, Aug. 2006. Morishima Masaki, Nagakatsu Harada, Hara Sayuri, Akira Takahashi and Yutaka Nakaya :
Hippocampal norepinephrine (NE) level and exercise behavior in SPORTS rats.,
15th Annual Meeting of the International Behavioral Neuroscience Society, Briitish Columbia, Canada, May 2006. Akiko Hamamoto, Masayuki Nakano, Toshitaka Ikehara, Masatake Akutagawa, Yohsuke Kinouchi, Yutaka Nakaya and Akira Takahashi :
A new water sterilization system which use UVA-light emitting diode (Q-401).,
106th ASM general meeting, Florida, USA, May 2006. Nakano Masayuki, Akira Takahashi, Sakai Yuko and Yutaka Nakaya :
Response of Vibrio parahaemolytiocus to neuroendcrine hormone norepinephrine (D-026).,
106th ASM general meeting, Florida,USA, May 2006. Nakano Masayuki, Akira Takahashi, Sakai Yuko and Yutaka Nakaya :
Norepinephrine modulates the pathogenicity of Vibrio parahameolyticus.,
11th Asian conference on diarrhoeal diseases and nutrition, Bangkok, Mar. 2006. Nakano Masayuki, Akira Takahashi, Sakai Yuko and Yutaka Nakaya :
Response of enteropathogen to neuroendocrine hormone .,
COE international conference on biological mechanism for stress control, Tokushima, Japan, Aug. 2005. Akira Takahashi, Nakano Masayuki, Miyoshi Shinichi, Nagakatsu Harada and Yutaka Nakaya :
(B-053) Hemolysin produced by Vibrio mimicus activates two Cl- secretory pathways in cultured intestinal-like cells.,
105th American society for microbiology general meeting, Atlanta, Georgia, USA, Jun. 2005. 中村美波, 松木大揮, ウラアナイツト, 内田貴之, 馬渡一諭, 髙橋章, 髙尾正一郎, 宮脇克行, 渡辺崇人, 三戸太郎, 栗木隆吉, 片岡孝介, 葦苅晟矢, 二川健 :
コオロギ摂食による栄養学的な機能性検討,
第78回日本栄養・食糧学会大会, 2024年5月. 白山優斗, 栗本一輝, 渡辺智貴, 岡本敏弘, 山口堅三, 上番増喬, 馬渡一諭, 髙橋章, 原口雅宣 :
ナノサイズ金属埋め込み円柱構造を大面積で作製する手法の検討,
第71回応用物理学会春季学術講演会, 22p-P06-5, 2024年3月. Duc Quang Tran, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Unraveling the Capacity and Mechanism of Gut Microbiota in Enhancing Essential Amino Acid Provision to Juvenile Mice Under Essential Amino Acid-Deficient Diet,
第268回徳島医学会学術集会(令和5年度冬季), Mar. 2024. 岸伸旺, 渡辺崇人, 井上慎太郎, 濱口汰暉, 石丸善康, 宮脇克行, 髙橋章, 二川健, 野地澄晴, 三戸太郎 :
フタホシコオロギにおけるクチクラ色素合成に関わる遺伝子の機能解析,
第68回応用動物昆虫学会, Vol.-, No.-, -, 2024年3月. 井上慎太郎, 渡辺崇人, 藤江快, 島村彩音, 石丸善康, 宮脇克行, 髙橋章, 二川健, 三戸太郎 :
コオロギをモデルとした昆虫の白色スクレロチン合成酵素遺伝子のメラニン生成制御機能の解析,
第68回応用動物昆虫学会, Vol.-, No.-, -, 2024年3月. 松岡実花, 武野香澄, 井上詩央里, 上番増喬, 馬渡一諭, 髙橋章 :
ケトジェニック食摂取時の血糖維持機構の解析,
第8回メタボローム解析シンポジウム, 2023年12月. 小野実優, 石田快, 牧本真奈, 下畑隆明, 上番増喬, 粟飯原睦美, 芥川正武, 榎本崇宏, 馬渡一諭, 岩田剛敏, 髙橋章 :
UVA 照射による Campylobacter jejuni の上皮定着性に対する影響,
第16 回日本カンピロバクター研究会総会, 2023年12月. 中山知彦, 梅原英裕, 富岡有紀子, 松本唯, 𠮷田朋広, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 髙橋章, 岡本泰昌, 沼田周助 :
大うつ病性障害患者における治療前後の血中代謝物濃度の変化,
第45回日本生物学的精神医学会年会, 2023年11月. 高木均, ナカガイトノリオアントニオ, Kawakami Nozomi, 髙橋章, 二川健 :
大豆廃棄物からのセルロースナノファイバーの抽出と評価,
第29回グリーンコンポジットWG会合および研究発表会要旨集, 4, 2023年10月. 馬渡一諭, 戸田沙慧, 平野希美, 結城史音, 上番増喬, 髙橋章 :
概日リズムを調節可能な低分子化合物の同定とその応用,
「プレシジョン栄養学の研究基盤確立を目指す食と栄養研究クラスター」「合成生物学に基づく産官学連携バイオエコノミー創薬プラットフォームの構築」研究クラスター若手合同ミーティング, 2023年10月. 戸田沙慧, 馬渡一諭, 殿脇壱成, 平野希美, 山口ももか, Bui Thi Kim Ngan, 石川寧子, 篠田浩一, 上番増喬, 髙橋章 :
柑橘由来ポリメトキシフラボンは新型コロナウイルスの宿主細胞内複製を抑制する,
第56回日本栄養·食糧学会中国·四国支部大会, 2023年10月. 相澤心太, 小井優萌那, 山下路代, 白石志帆, 宮脇克行, 粟飯原睦美, 二川健, 上番増喬, 馬渡一諭, 髙橋章 :
極地環境での大豆栽培方法の確立,
第56回日本栄養·食糧学会中国·四国支部大会, 2023年10月. 中山知彦, 梅原英裕, 富岡有紀子, 松本唯, 𠮷田朋広, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 髙橋章, 岡本泰昌, 沼田周助 :
双極性障害における血中代謝物の変化,
第33回日本臨床精神神経薬理学会学術集会, 2023年9月. 牧本真奈, 福島志帆, 山中咲季, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni病原性に対するコハク酸の影響,
第44回日本食品微生物学会学術総会, 2023年9月. Duc Quang Tran, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Unraveling the Capacity and Mechanism of Gut Microbiota in Enhancing Essential Amino Acid Provision to Juvenile Mice Under Essential Amino Acid-Deficient Diet,
2023 Tokushima Bioscience Retreat, Sep. 2023. 馬渡一諭, 髙橋章 :
光殺菌の波長依存性,
日本防菌防黴学会第50回年次大会, 2023年8月. Bui Kim Thi Ngan, Kazuaki Mawatari, SHINODA Koichi, 平川仁, 粟本健司, 脇谷正幸, 篠田傳 and Akira Takahashi :
Determination of optimum wavelength of far-UVC for virucidal and bactericidal effects using plasma emission-based light modules,
日本防菌防黴学会第50回年次大会, Aug. 2023. 篠田浩一, 馬渡一諭, Bui Thi Kim Ngan, 平川仁, 粟本健司, 脇谷正幸, 篠田傳, 髙橋章 :
人にも環境にもやさしい光殺菌を目指した波長制御型Far-UVC光源の開発,
日本防菌防黴学会第50回年次大会, 2023年8月. 斧田優志, 石田快, 石川寧子, 田中佐保, 山下路代, 福島志帆, 相澤俊彦, 山内繁晴, 藤川康夫, 田中智毅, 上番増喬, 馬渡一諭, 髙橋章 :
UV-LEDの光学特性に適したUV感受性評価のための標準化光源の開発,
日本防菌防黴学会第50回年次大会, 2023年8月. 石田快, 斧田優志, 石川寧子, 田中佐保, 山下路代, 福島志帆, 相澤俊彦, 山内繁晴, 藤川康夫, 田中智毅, 上番増喬, 馬渡一諭, 髙橋章 :
ウイルスに対する波長依存的不活化効果の評価,
日本防菌防黴学会第50回年次大会, 2023年8月. 馬渡一諭, 小池宣也, 野原一成, 敷島康普, 三浦宏之, 新居佳孝, 上番増喬, 下畑隆明, 八木田和弘, 髙橋章, Yoo Seung-Hee, Chen Zheng :
スダチ果皮特有のフラボノイド・スダチチンの概日リズム調節作用と肝脂質代謝改善作用,
第267回徳島医学会学術集会, 2023年8月. 馬渡一諭, 小池宣也, 野原一成, 敷島康普, 三浦宏之, 新居佳孝, 上番増喬, 下畑隆明, 八木田和弘, 髙橋章, Seung-Hee Yoo, Zheng Chen :
スダチ由来ポリメトキシフラボン・スダチチンの概日リズム調節作用と肝脂質代謝改善作用,
第77回日本栄養・食糧学会大会, 2023年5月. 武野香澄, 上番増喬, 相澤心太, 下畑隆明, 馬渡一諭, 髙橋章 :
母体のケトジェニック食摂取が仔の脂質代謝に与える影響,
2023年5月. 戸田沙慧, 馬渡一諭, 古家光二, 殿脇壱成, BUI THI KIM NGAN, 石川寧子, 篠田浩一, 上番増喬, 髙橋章 :
概日リズムを調節可能な化合物によるヒトコロナウイルス複製抑制効果,
第77回日本栄養・食糧学会大会, 2023年5月. 松木大揮, 鈴木穂, 鴻野まどか, ウラアナイツト, 馬渡一諭, 髙橋章, 宮脇克行, 渡辺崇人, 三戸太郎, 栗木隆吉, 二川健 :
コオロギの抗筋萎縮作用について,
第77回日本栄養・食糧学会大会, 2023年5月. 井上慎太郎, 渡辺崇人, 濱口汰暉, 石丸善康, 宮脇克行, 二川健, 髙橋章, 野地澄晴, 三戸太郎 :
コオロギをモデルとした体色パターン制御の分子メカニズムの解析,
第67回日本応用動物昆虫学会, 2023年3月. 戸田沙慧, 馬渡一諭, 殿脇壱成, 平野希美, 山口ももか, Bui Thi Kim Ngan, 石川寧子, 篠田浩一, 上番増喬, 髙橋章 :
ポリメトキシフラボンのノビレチンはコロナウイルスの宿主細胞内複製を抑制する,
徳島大学大学院医歯薬学研究部 2023 感染・免疫クラスター・ミニリトリート「生命科学・医工連携リトリートならびに教育クラスターによる分野横断的大学院教育の促進」, 2023年2月. 濱口汰暉, 井上慎太郎, 宮脇克行, 髙橋章, 二川健, 石丸善康, 野地澄晴, 渡辺崇人, 三戸太郎 :
フタホシコオロギにおける色素合成に関わる遺伝子の機能解析,
第45回日本分子生物学会, 2022年12月. 福島志帆, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章 :
リソソームはCampylobacter jejuniの宿主細胞内生存に寄与する,
第95回日本生化学会大会, 2022年11月. 石田快, 粟飯原睦美, 下畑隆明, 北山栞里, 勢川玲花, 上番増喬, 馬渡一諭, 鈴木浩司, 芥川正武, 榎本崇宏, 山本光生, 富久章子, 和田敬宏, 岡本雅之, 伊藤浩, 安野卓, 木内陽介, 髙橋章 :
鶏舎におけるUV-LED導入による衛生環境改善効果の検討,
第43回日本食品微生物学会学術総会, 2022年9月. 石田快, 粟飯原睦美, 下畑隆明, 北山栞里, 勢川玲花, 上番増喬, 馬渡一諭, 鈴木浩司, 芥川正武, 榎本崇宏, 山本光生, 富久章子, 和田敬宏, 岡本雅之, 伊藤浩, 安野卓, 木内陽介, 髙橋章 :
鶏舎内へのUV―LED導入による鶏の生育及び衛生環境の改善効果の検討,
日本家禽学会2022年度秋季大会, 2022年9月. Bui Kim Thi Ngan, Kazuaki Mawatari, Takahiro Emoto, Shiho Fukushima, Takashi Uebanso, Takaaki Shimohata, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak,
第265回徳島医学会学術集会, Jul. 2022. 松木大揮, 山崎穂, 鴻野まどか, 中野亘, ANAYTULLA (名), 髙橋章, 宮脇克行, 渡辺崇人, 三戸太郎, 栗木隆吉, 二川健 :
コオロギの抗筋萎縮作用について,
第76回日本栄養・食糧学会大会, 2022年6月. 石田快, 下畑隆明, 佐野真梨奈, 射場仁美, 上番増喬, 馬渡一諭, 髙橋章 :
菌体外NaCl濃度変化に応答する，腸炎ビブリオの病原性解析,
第76回日本栄養・食糧学会大会, 2022年6月. 射場仁美, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章 :
Vibrio parahaemolyticusの病原因子T3SS1遺伝子発現は宿主細胞接着によって誘導される,
第76回日本栄養・食糧学会大会, 2022年6月. 上番増喬, 相澤心太, 中村真彩, 須山真衣, 吉本亜由美, 増田瑠見子, 下畑隆明, 馬渡一諭, 髙橋章 :
ビタミンB2の栄養状態と高シュウ酸尿症との関係,
第76回日本栄養・食糧学会大会, 2022年6月. 北山栞里, 下畑隆明, 白石志帆, 石田快, 緒方美裕起, 上番増喬, 馬渡一諭, 粟飯原睦美, 芥川正武, 榎本崇宏, 鈴木浩司, 安野卓, 伊藤浩, 和田敬宏, 岡本雅之, 富久章子, 髙橋章, 木内陽介 :
UV-LEDによる鶏舎内光環境の構築,
LED総合フォーラム2022 in 徳島, 169-172, 2022年1月. Toshitaka Ikehara, Mutsumi Aihara, Koichiro Tsuchiya, Takahiro Emoto, Masatake Akutagawa, Akira Takahashi and Yohsuke Kinouchi :
Studies of reactive oxygen species scavenging system of cultured cells by using LED light irradiation,
LED総合フォーラム2022 in 徳島, 193-198, Jan. 2022. 天宅あや, 栗栖修作, 髙橋章, 米村重信 :
上皮基底膜 IV型コラーゲンの動態解析,
第94回日本生化学会大会, 2021年11月.

(キーワード): 細胞外マトリックス (extracellular matrix) / コラーゲン (collagen) / ライブイメージング

天宅あや, 栗栖修作, 髙橋章, 米村重信 :
間質細胞由来IV型コラーゲンががん細胞に与える影響,
第262回徳島医学会学術集会, 2021年3月.

(キーワード): 基底膜 / IV型コラーゲン

原口雅宣, 北田貴弘, 永瀬雅夫, 安井武史, 木内陽介, 宮脇克行, 髙橋章, 岡久稔也 :
2019年度におけるLEDライフイノベーション総合プラットフォーム推進事業の取り組み,
LED総合フォーラム2020in徳島, 75-76, 2020年2月.

(キーワード): LEDライフイノベーション / LED / テラヘルツ

石田快, 下畑隆明, 神田結奈, 増田瑠見子, 上番増喬, 馬渡一諭, 柏本孝茂, 髙橋章 :
Metabolism changing of Vibrio vulnificus infected tissue in wound infection model mouse,
第93回日本細菌学会総会, 2020年2月. 近藤翼, 荒尾菜月, 上番増喬, 下畑隆明, 馬渡一諭, 髙橋章 :
炎症性腸疾患における味覚受容体T1R3の役割の解析,
第260回徳島医学会学術集会, 2020年2月. 福島志帆, 下畑隆明, 木戸純子, 上番増喬, 馬渡一諭, 髙橋章 :
オートファジーを介したCampylobacter jejuni侵入機構へのRac GTPaseの関与,
第260回徳島医学会学術集会, 2020年2月. 下畑隆明, 木戸純子, 鳴滝涼香, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
代謝産物の網羅的解析から紐解く，Campylobacter jejuniの生存戦略,
第260回徳島医学会学術集会, 2020年2月. 相澤心太, 上番増喬, 下畑隆明, 馬渡一諭, 髙橋章 :
母親の腸内細菌叢の変化が仔の食物アレルギー発症に与える影響,
第72回日本細菌学会中国・四国支部総会, 2019年11月. 二川健, 髙橋章, 宮脇克行 :
機能性宇宙食,
第63回宇宙科学技術連合講演会, 2019年11月. 下畑隆明, 木戸純子, 福島志帆, 天宅あや, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染は宿主上皮細胞のアミノ酸取り込みを促進する,
第92回日本生化学会大会, 2019年9月. Ulfa Maria, Takaaki Shimohata, Shiho Fukushima, Masami Sato, Momoyo Azuma, Takashi Uebanso, Kazuaki Mawatari, Masatake Akutagawa, Yohsuke Kinouchi and Akira Takahashi :
Effect of 365 nm LED on the inactivation of ESBL producing Escherichia coli,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, Aug. 2019. 福島志帆, 下畑隆明, 木戸純子, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni induce autophagy for the bacterial invasion in the host epithelial cells,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2019年8月. 福井萌加, 上番増喬, 内藤千里, 増田瑠見子, 下畑隆明, 馬渡一諭, 髙橋章 :
腸管上皮細胞におけるケトン体代謝調節機構,
第73回日本栄養・食糧学会大会, 2019年5月. Ulfa Maria, Takaaki Shimohata, Shiho Fukushima, Momoyo Azuma, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
The effectiveness of UVA-LED irradiation on ESBL producing Escherichia coli,
第92回日本細菌学会総会, Apr. 2019. 石田快, 下畑隆明, 畑山翔, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Effect of cholesterol on Campylobacter jejuni survival in host intestinal epithelial cells,
第92回日本細菌学会総会, 2019年4月. 福島志帆, 下畑隆明, 木戸純子, 石田快, 上番増喬, 馬渡一諭, 髙橋章 :
オートファジーの誘導はCampylobacter jejuniの宿主細胞への侵入過程を促進する,
第92回日本細菌学会総会, 2019年4月. 中尾玲子, 宮脇克行, 出口祥啓, 髙橋章, 二川健 :
宇宙栄養・食糧関連技術の開発とGatewayへの期待,
国際宇宙探査ワークショップ(その2), 2019年3月. 森大樹, 森根裕二, 横田典子, 石橋広樹, 馬渡一諭, 髙橋章, 島田光生 :
膵・胆管合流異常における発がんメカニズムに関する検討,
第80回日本臨床外科学会総会, 2018年11月. 福島志帆, 下畑隆明, 木戸純子, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染で誘導されるオートファジーは宿主細胞への菌の侵入および細胞内生存を促進する,
第71回日本細菌学会中国・四国支部総会, 2018年10月. 神田結奈, 東山諒, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 福島志帆, 石田快, 上番増喬, 馬渡一諭, 柏本孝茂, 髙橋章 :
Vibrio Vulnificusが産生するOMVの機能解析,
第71回日本細菌学会中国・四国支部総会, 2018年10月. 天宅あや, 下畑隆明, 畑山翔, NGUYEN QUOC ANH, 木戸純子, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染によって誘導される小胞体ストレス応答は菌の細胞侵入を抑制する,
第39回日本食品微生物学会学術総会, 2018年9月. 畑山翔, 下畑隆明, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 中本晶子, 首藤恵泉, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni汚染調理器具に対するUVA-LED照射殺菌の有効性について,
第39回日本食品微生物学会学術総会, 2018年9月. 齋藤裕, 森大樹, 森根裕二, 矢田圭吾, 石橋広樹, 馬渡一論, 髙橋章, 島田光生 :
メタボローム解析を用いた膵・胆管合流異常の胆汁中発癌物質の同定,
第54回日本胆道学会学術集会, 2018年9月. 神田結奈, 東山諒, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 福島志帆, 石田快, 上番増喬, 馬渡一諭, 柏本孝茂, 髙橋章 :
Vibrio Vulnificusが産生するOMVの機能解析,
第39回日本食品微生物学会学術総会, 2018年9月. 福島志帆, 下畑隆明, 木戸純子, 上番増喬, 馬渡一諭, 髙橋章 :
オートファジーはCampylobacter jejuniの宿主細胞への侵入過程を促進する,
第91回日本生化学会大会, 2018年9月. 下畑隆明, 畑山翔, 木戸純子, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniは宿主腸管上皮細胞の細胞側面から効率的に侵入する,
第91回日本生化学会大会, 2018年9月. Anh Quoc Nguyen, Takaaki Shimohata, Anh Quoc Nguyen, Sho Hatayama, Aya Tentaku, Junko Kido, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Metabolic changing in epithelial cell during Vibrio parahaemolyticus infection,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, Aug. 2018. 天宅あや, 下畑隆明, NGUYEN QUOC ANH, 畑山翔, 木戸純子, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Unfolded Protein Response Suppressed Campylobacter jejuni-invasion in Epithelial Cells,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2018年8月. Ulfa Maria, Takaaki Shimohata, Shiho Fukushima, Momoyo Azuma, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Application of UVA-LED on Extended-Spectrumβ-Lactamase(ESBL) Producing Escherichia coli from Clinical Isolates,
第257回徳島医学会学術集会, Aug. 2018. 石田快, 下畑隆明, 木戸純子, 天宅あや, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの宿主腸管上皮細胞での生存に対するコレステロールの影響,
第92回日本感染症学会学術講演会, 2018年5月. 福島志帆, 下畑隆明, 木戸純子, 天宅あや, 石田快, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染で誘導されるオートファジーは宿主細胞への侵入および細胞内生存を促進する,
第92回日本感染症学会学術講演会, 2018年5月. 森大樹, 森根裕二, 矢田圭吾, 石橋広樹, 馬渡一諭, 髙橋章, 島田光生 :
メタボローム解析を用いた膵・胆管合流異常における胆汁発癌物質の検討,
第55回日本小児外科学会学術集会, 2018年5月. 天宅あや, 下畑隆明, 畑山翔, NGUYEN QUOC ANH, 木戸純子, 福島志帆, 石田快, 上番増喬, 馬渡一諭, 髙橋章 :
宿主細胞内での小胞体ストレス応答(UPR)はCampylobacter jejuni感染に対する防御機構として働く,
第72回日本栄養・食糧学会大会, 2018年5月. 森大樹, 森根裕二, 矢田圭吾, 石橋広樹, 馬渡一諭, 髙橋章, 島田光生 :
メタボローム解析を用いた膵・胆管合流異常の胆汁中発癌物質の同定,
第118回日本外科学会定期学術集会, 2018年4月. Quoc Nguyen anh, Takaaki Shimohata, sho Hatayama, aya Tentaku, Junko kido, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Metabolic analysis of epithelial cellular metabolism during Vibrio parahaemolyticus infection,
第91回日本細菌学会総会, Mar. 2018. 天宅あや, 下畑隆明, 畑山翔, NGUYEN QUOC ANH, 木戸純子, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染における小胞体ストレス応答は菌の侵入を抑制する,
第91回日本細菌学会総会, 2018年3月. 福島志帆, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 神田結奈, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniは菌の宿主上皮細胞への侵入および生存過程にオートファジーを利用する,
第91回日本細菌学会総会, 2018年3月. 福島志帆, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染で誘導されるオートファジーは，宿主上皮細胞における菌の侵入および生存を促進する,
第256回徳島医学会学術集会, 2018年2月. 木戸純子, 下畑隆明, 天野幸恵, 畑山翔, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
CFTR発現はCampylobacter jejuniの微小管を介した輸送を抑制する,
2017年度生命科学系学会合同年次大会, 2017年12月. 天宅あや, 下畑隆明, 畑山翔, NGUYEN QUOC ANH, 木戸純子, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染で誘導される小胞体ストレス応答は細胞内への菌の侵入を抑制する,
2017年度生命科学系学会合同年次大会, 2017年12月. 福島志帆, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染で誘導されるオートファジーは，宿主上皮細胞における菌の侵入および生存を促進する,
2017年度生命科学系学会合同年次大会, 2017年12月. 下畑隆明, 木戸純子, 畑山翔, 上番増喬, 馬渡一諭, 髙橋章 :
代謝産物の解析から紐解くCampylobacter jejuniの宿主細胞内生存戦略,
第70回日本細菌学会中国・四国支部総会, 2017年10月. Anh Quoc Nguyen, Takaaki Shimohata, Sho Hatayama, Aya Tentaku, Junko Kido, Takashi Uebanso, Kazuaki Mawatari and Akira Takahashi :
Metabolic analysis of epithelial cellular metabolism during Vibrio parahaemolyticus infection,
第70回日本細菌学会中国・四国支部総会, Oct. 2017. 天宅あや, 下畑隆明, 畑山翔, NGUYEN QUOC ANH, 木戸純子, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Cpylobacter jejuni感染によって誘導される小胞体ストレス応答が菌に与える影響,
第70回日本細菌学会中国・四国支部総会, 2017年10月. 畑山翔, 下畑隆明, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
極性化腸管上皮細胞におけるタイトジャンクション形成はCampylobacter jejuniの細胞内侵入機構に影響する,
第38回日本食品微生物学会学術総会, 2017年10月. 福島志帆, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 神田結奈, 鳴滝涼香, 石田快, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染で誘導されるオートファジーは菌のクリアランス機構として働くのか,
第38回日本食品微生物学会学術総会, 2017年10月. 本山裕隆, 芥川正武, 榎本崇宏, 安野恵実子, 下畑隆明, 髙橋章, 小中信典, 木内陽介 :
有限要素法を用いたヒト腸管インピーダンス測定用電極の検討,
平成29年度電気関係学会四国支部連合大会講演論文集(2017 愛媛大学), 160, 2017年9月. 木戸純子, 下畑隆明, 天野幸恵, 畑山翔, 佐藤優里, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
CFTR reduced microtubule-mediated Campylobacter jejuni invasion,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2017年8月. 上番増喬, 大西愛, 北山礼子, 吉本亜由美, 中橋睦美, 下畑隆明, 馬渡一諭, 髙橋章 :
許容上限量の甘味料の摂取が腸内細菌叢と宿主へ及ぼす影響の解析,
日本栄養食糧学会, 243, 2017年5月. 畑山翔, 下畑隆明, 天野幸恵, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
宿主腸管上皮細胞のTight Junction形成が食中毒原因菌Campylobacter jejuniの侵入機構に及ぼす影響について,
日本栄養食糧学会, 244, 2017年5月. 天宅あや, 下畑隆明, 木戸純子, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染における小胞体ストレスの誘導,
第91回日本感染症学会総会・学術講演会, 2017年4月. 畑山翔, 下畑隆明, 天野幸恵, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
極性化腸管上皮細胞でのタイトジャンクションによるCampylobaster Jejuniの侵入制御について,
日本細菌学会総会, 50, 2017年3月. 下畑隆明, 木戸純子, 佐藤優里, 畑山翔, 神田結奈, 福島志帆, 天宅あや, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni 感染はHeLa細胞におけるアミノ酸取り込みを活性化する,
日本細菌学会総会, 50, 2017年3月. 馬渡一諭, 安井実希, 本庄アイリ, 西坂理沙, 枝川美幸, 岩本夏美, 渡邊瞳, 下畑隆明, 上番増喬, 髙橋章 :
Screening of genes related with ultraviolet A-sensitivity by transposon mutagenesis in Vibrio parahaemolyticus,
90th Annual Meeting of Japanese Society of bacteriology, 46, 2017年3月. 上番増喬, 吉本亜由美, 下畑隆明, 中橋睦美, 馬渡一諭, 髙橋章 :
食事成分の腸内細菌叢への影響を代謝産物から読み解けるか?,
日本細菌学会総会, 42, 2017年3月. 宇山真由, 曽我部正弘, 平田光里, 中川忠彦, 福家慧, 寺前智史, 藤本大策, 田中宏典, 三井康裕, 北村晋志, 岡本耕一, 高山哲治, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 榎本崇宏, 芥川正武, 片島るみ, 岡久稔也 :
模擬腹水による胸腹水濾過濃縮再静注法の教育体制の構築,
第37回日本アフェレシス学会学術大会, 2016年11月. 玉井瑠人, 笠井嘉人, 田中大基, 谷直也, 平田光里, 中川忠彦, 友成哲, 谷口達哉, 岡本耕一, 宮本弘志, 高山哲治, 榎本崇宏, 芥川正武, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 宇山真由, 曽我部正弘, 岡久稔也 :
胸腹水濾過濃縮再静注法のクロスフロー方式の腹水濾過時の物理的刺激が腹水に及ぼす影響に関する実験的検討.,
第54回日本人工臓器学会大会, 2016年11月. 渡邊瞳, 馬渡一諭, 西坂理沙, 中橋睦美, 常冨愛香里, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
透析液の細菌汚染調査と近視外LEDによる殺菌効果の評価,
第69回日本細菌学会中国・四国支部総会, 2016年10月. 下畑隆明, 福島志帆, 佐藤優里, 扶川留音, 木戸純子, 神田結奈, 天宅あや, 畑山翔, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの宿主細胞内生存戦力に関する検討,
第69回日本細菌学会中国・四国支部総会, 2016年10月. 木戸純子, 下畑隆明, 天野幸恵, 畑山翔, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
CFTR発現によるCampylobacter jejuniの侵入抑制機序の解明,
第69回日本細菌学会中国・四国支部総会, 2016年10月. 中橋睦美, 常冨愛香里, 上番増喬, 下畑隆明, 馬渡一諭, 芥川正武, 木内陽介, 髙橋章 :
紫外線LEDと次亜塩素酸ナトリウムの併用による食品殺菌装置の開発,
第37回日本食品微生物学会学術総会, 2016年9月. 髙橋章, 下畑隆明, 馬渡一諭, 上番増喬, 常冨愛香里, 中橋睦美, 芥川正武, 木内陽介 :
飲料水の色が近紫外線殺菌にあたえる影響,
第37回日本食品微生物学会学術総会, 2016年9月. 常冨愛香里, 下畑隆明, 永田早紀恵, 天野幸恵, 中橋睦美, 原田優美, 上番増喬, 馬渡一諭, 宮脇克行, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
Campylobacter jejuni食中毒予防に対するUVA-LED照射殺菌の有用性について,
第37回日本食品微生物学会学術総会, 2016年9月. 下畑隆明, 福島志帆, 佐藤優里, 扶川留音, 木戸純子, 神田結奈, 天宅あや, 畑山翔, 中橋睦美, 上番増喬, 原田永勝, 馬渡一諭, 髙橋章 :
上皮細胞に侵入したCampylobacter jejuniのエネルギー獲得機構について,
第37回日本食品微生物学会学術総会, 2016年9月. 畑山翔, 下畑隆明, 根来幸恵, 木戸純子, 射場仁美, 上番増喬, 馬渡一諭, 髙橋章 :
腸管上皮細胞におけるtight junctionsの破綻は細胞側面からの脂質ラフトを介したCampylobacter jejuniの侵入を促進する,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2016年8月. 西坂理沙, 渡邊瞳, 馬渡一諭, 中橋睦美, 常冨愛香里, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
県内医療施設の透析液細菌汚染調査と近紫外LEDによる殺菌効果の評価,
第253回徳島医学会学術集会, 2016年7月. 木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
CFTR発現によりCampylobacter jejuniの微小管依存性侵入機構が抑制される,
第253回徳島医学会学術集会, 2016年7月. 吉本亜由美, 上番増喬, 下畑隆明, 中橋睦美, 馬渡一諭, 髙橋章 :
妊娠期の母親の低用量の抗菌薬摂取が子供の健康に及ぼす影響の解析,
第253回徳島医学会学術集会, 24, 2016年7月. 畑山翔, 下畑隆明, 吉兼道子, 天野幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
食中毒原因菌Campylobacter jejuniは腸管上皮細胞側面の露出により宿主内へ効率的に侵入する,
第70回日本栄養・食糧学会大会, 2016年5月. 常冨愛香里, 下畑隆明, 後藤茉凜, 天野幸恵, 中橋睦美, 原田優美, 上番増喬, 馬渡一諭, 宮脇克行, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
UVA-LED殺菌システムによるCampylobacter jejuni食中毒の予防,
第70回日本栄養・食糧学会大会, 2016年5月. 宇山真由, 曽我部正弘, 平田光里, 中川忠彦, 高岡慶史, 谷口達哉, 高山哲治, 榎本崇宏, 芥川正武, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 岡久稔也 :
胸腹水濾過濃縮再静注法の手技習得のための輸液製剤を用いた模擬腹水の作成,
第43回日本血液浄化技術学会学術大会, 2016年5月.

(キーワード): 胸腹水濾過濃縮再静注法

常冨愛香里, 木戸純子, 上番増喬, 下畑隆明, 馬渡一諭, 髙橋章 :
UVA殺菌技術を用いた植物工場における病原微生物の抑制検討,
第90回日本感染症学会総会・学術講演会, 2016年4月. 西坂理沙, 馬渡一諭, 山下智子, 木戸純子, 常冨愛香里, 下畑隆明, 上番増喬, 髙橋章 :
透析液の汚染細菌調査と近紫外発光ダイオードを用いた殺菌効果の評価,
第90回日本感染症学会総会・学術講演会, 2016年4月. 木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 佐藤優里, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの細胞侵入は細胞膜表面CFTRにより調節される,
第89回日本細菌学会総会, 2016年3月. 下畑隆明, 佐藤優里, 扶川留音, 木戸純子, 天野幸恵, 畑山翔, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
代謝解析で明らかとなったCampylobacter jejuni感染による宿主細胞での特徴的なアミノ酸変動について,
第89回日本細菌学会総会, 2016年3月. 吉本亜由美, 上番増喬, 中橋睦美, 下畑隆明, 馬渡一諭, 髙橋章 :
Effect of low dose antibiotics administration during prenatal period on newborns postnatal health,
第89回日本細菌学会総会, 2016年3月. 馬渡一諭, 枝川美幸, 岩本夏実, 前谷実希, 本庄アイリ, 西坂理沙, 渡邊瞳, 下畑隆明, 上番増喬, 髙橋章 :
Identification of genes associated with ultraviolet-A sensitivity in Vibrio parahaemolyticus,
第89回日本細菌学会総会, 2016年3月. 西坂理沙, 馬渡一諭, 常冨愛香里, 渡邊瞳, 上番増明子, 下畑隆明, 上番増喬, 金本優杞, 村上明男, 髙橋章 :
Ultraviolet-A (UVA) irradiation by light emitting diode (LED) inhibits growth of cyanobacteria,
第89回日本細菌学会総会, 2016年3月. 谷直也, 田中大基, 末内辰尚, 高岡慶史, 中川忠彦, 高山哲治, 河野正樹, 榎本崇宏, 芥川正武, 木内陽介, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 宇山真由, 曽我部正弘, 岡久稔也 :
イノベーション対話ツールを活用した腸蠕動音収集解析システムの開発,
第53回日本人工臓器学会大会, 2015年11月. 田中大基, 谷直也, 武原正典, 田中宏典, 田中貴大, 友成哲, 谷口達哉, 中川忠彦, 高山哲治, 河野正樹, 榎本崇宏, 芥川正武, 木内陽介, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 宇山真由, 曽我部正弘, 岡久稔也 :
模擬腹水を用いた胸腹水濾過濃縮装置の評価・教育システムの構築,
第53回日本人工臓器学会大会, 2015年11月. 河野正樹, 榎本崇宏, 芥川正武, 宇山真由, 曽我部正弘, 中川忠彦, 高山哲治, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 村島徹, 出口喜宏, 荒巻広至, 岡久稔也 :
腹水濾過濃縮法(CART)用装置の現状と今後,
第36回日本アフェレシス学会学術総会, 2015年10月. 宮脇克行, 常冨愛香里, 後藤茉凜, 正村彰規, 髙橋章, 野地澄晴 :
完全人工光型植物工場におけるイチゴ苗大量生産システムの開発,
園芸学会, 2015年9月.

(キーワード): 完全人工光型植物工場 / イチゴ / UVA-LED殺菌

木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 佐藤優里, 扶川留音, 吉兼道子, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの上皮細胞侵入性に対するCFTRの関与について,
第251回徳島医学会学術集会, 2015年8月. 常冨愛香里, 正村彰規, 中橋睦美, 西坂理沙, 後藤茉凛, 馬渡一諭, 下畑隆明, 上番増喬, 宮脇克行, 芥川正武, 木内陽介, 髙橋章 :
植物工場の養液殺菌システムの開発,
第251回徳島医学会学術集会, 2015年8月. 西坂理沙, 馬渡一諭, 常冨愛香里, 後藤茉凛, 上番増明子, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 金本優杞, 村上明男, 髙橋章 :
近紫外線(ultrviolet-A)照射によるシアノバクテリア増殖抑制効果の解明,
第251回徳島医学会学術集会, 2015年8月. 畑山翔, 下畑隆明, 吉兼道子, 根来幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの上皮細胞への侵入は細胞のラテラル面の露出により増加する,
第251回徳島医学会学術集会, 2015年8月. 下畑隆明, 木戸純子, 根来幸恵, 畑山翔, 佐藤優里, 射場仁美, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
CFTR regulates Campylobacter jejuni invasion in cultured cells,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会, 2015年7月. 山下智子, 馬渡一諭, 木戸純子, 下畑隆明, 髙橋章 :
人口透析装置汚染防止を目的とした近紫外発光ダイオード(UVA-LED)による照射殺菌条件の検討,
第89回日本感染症学会総会・学術講演, 2015年4月. 木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 上番増喬, 馬渡一諭, 髙橋章 :
CFTRがCampylobacter jejuniの細胞侵入性へ与える影響,
第89回日本感染症学会総会・学術講演, 2015年4月. 馬渡一諭, 本庄アイリ, 安井実希, 畑山翔, 中橋睦美, 下畑隆明, 髙橋章 :
腸炎ビブリオのVP2357遺伝子変異は近紫外線(UVA)照射耐性獲得に寄与する,
第88回日本細菌学会総会, 2015年3月. Quang Ngoc Phan, Takashi Uebanso, Kazuaki Mawatari, Takaaki Shimohata, Mutsumi Nakahashi and Akira Takahashi :
DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus,
第88回日本細菌学会総会, Mar. 2015. 畑山翔, 下畑隆明, 根来幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniは腸管上皮の側部より効率的に侵入する,
第88回日本細菌学会総会, 2015年3月. Phan Quang Ngoc, Takashi Uebanso, Kazuaki Mawatari, Takaaki Shimohata, Mutsumi Nakahashi and Akira Takahashi :
DNA-binding protein HU coordinate pathogenicity in Vibrio parahaemolyticus,
第67回日本細菌学会中国・四国支部総会, Oct. 2014. 常冨愛香里, 中橋睦美, 西坂理沙, 馬渡一諭, 下畑隆明, 上番増喬, 宮脇克行, 芥川正武, 木内陽介, 髙橋章 :
水耕栽培用養液の還流型殺菌システムの開発,
第67回日本細菌学会中国・四国支部総会, 2014年10月. 木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 佐藤優里, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniによる細胞侵入とCFTRの関連について,
第67回日本細菌学会中国・四国支部総会, 2014年10月. 矢野佑樹, 芥川正武, 榎本崇宏, 小中信典, 小中信典, 木内陽介, 下畑隆明, 髙橋章, 安野恵実子 :
有限要素法を用いたマウス腸管のインピーダンス測定方法の検討,
電気関係学会四国支部連合大会講演論文集, No.14-10, 238, 2014年9月.

(要約): クローン病などの腸の疾患で腸管の電気インピーダンスが健常者と有病者とで異なることが知られている．本研究では電気インピーダンスを用いた腸疾患の診断を目的として，マウスの腸管インピーダンスを測定する際の電極形状について，有限要素法を用いて基礎研究を行っている．

星山哲平, 芥川正武, 榎本崇宏, 中橋睦美, 林田麻里王, 髙橋章, 馬渡一諭, 池原敏孝, 小中信典, 木内陽介 :
UV及び可視光照射に対する腸炎ビブリオの遺伝子発現解析,
電気関係学会四国支部連合大会講演論文集, No.14-1, 229, 2014年9月.

(要約): 腸炎ビブリオを紫外線および可視光に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．その結果，UV-A領域の光を照射した際，酸化修復に関する遺伝子の働きが抑えられること，緑色の光を照射した際に鞭毛関連の遺伝子が活性化することなどが示された．

本庄アイリ, 馬渡一諭, 前谷実希, 岩本夏実, 山下智子, 中橋睦美, 下畑隆明, 上番増喬, 髙橋章 :
Anti-sigma factor VP2357の遺伝子変異はVibrio parahaemolyticusの近紫外線(UVA)耐性獲得に関与する,
第249回徳島医学会学術集会, 2014年7月. Phan Quang Ngoc, Takashi Uebanso, Kazuaki Mawatari, Takaaki Shimohata, Mutsumi Aihara and Akira Takahashi :
DNA-binding protein HU coordinates pathogenicity in Vibrio parahaemolyticus.,
第249回徳島医学会学術集会, Jul. 2014. 畑山翔, 下畑隆明, 根来幸恵, 佐藤優里, 木戸純子, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni 感染による腸管上皮細胞のイオン輸送に関する検討,
第249回徳島医学会学術集会, 2014年7月. 星山哲平, 芥川正武, 榎本崇宏, 中橋睦美, 林田麻里王, 髙橋章, 馬渡一諭, 池原敏孝, 小中信典, 木内陽介 :
異なる波長の光照射に対する腸炎ビブリオの遺伝子発現解析,
電子情報通信学会技術研究報告(MEとバイオサイバネティックス), Vol.114, No.154, 19-24, 2014年7月.

(要約): 本研究は細菌へどのような光を照射すると，どのような機能が活性されるかについて考察するものである．現在，光照射が細菌にもたらす影響が多数報告されており，例えば紫外(UV)照射を行う事で殺菌効果が得られ，可視光照射では光受容体という機能の存在が示されている．本研究では，このような異なる波長の光照射に対する細菌への影響を，遺伝子発現解析により考察した．その結果， UV及び可視光の各波長の光照射時について変動の見られた腸炎ビブリオ遺伝子を特定する事ができた．また， 520nmの光を腸炎ビブリオに照射すると，腸炎ビブリオの鞭毛遊走機能が活性化される可能性が示唆された．
(キーワード): 光照射 / 波長依存性 / 遺伝子発現解析 / 腸炎ビブリオ

常冨愛香里, 正村彰規, 粟飯原睦美, 馬渡一諭, 下畑隆明, 上番増喬, 石崎仁愛, 芥川正武, 木内陽介, 髙橋章 :
近紫外線による水耕栽培用培養液の殺菌法の開発,
第68回日本栄養・食糧学会大会, 2014年5月. 本庄アイリ, 馬渡一諭, 前谷実希, 常冨愛香里, 畑山翔, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 :
Vibrio parahaemolyticusのkatGgene, VPA0768発現上昇は近紫外線(UVA)耐性獲得に関与する,
第68回日本栄養・食糧学会大会, 2014年5月. 根耒幸恵, 下畑隆明, 畑山翔, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni 感染はCFTR機能を抑制する,
第68回日本栄養・食糧学会大会, 2014年5月. 馬渡一諭, 本庄アイリ, 常冨愛香里, 畑山翔, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 :
近紫外線(UVA)照射により酸化される腸炎ビブリオタンパク質の同定,
第87回日本細菌学会総会, 2014年3月. 下畑隆明, 根耒幸恵, 畑山翔, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染はT84細胞におけるCl-分泌を抑制する,
第87回日本細菌学会総会, 2014年3月. 工藤隆治, 藤澤健司, 工藤景子, 小林真左子, 栗林伸行, 髙丸菜都美, 大江剛, 内田大亮, 髙橋章, 玉谷哲也, 永井宏和, 生島仁史, 宮本洋二 :
当科における強度変調放射線治療(IMRT)の経験,
第12回中国四国口腔癌研究会学術講演会, 2013年11月. 林田麻里王, 粟飯原睦美, 上番増喬, 下畑隆明, 馬渡一諭, 髙橋章, 星山哲平, 芥川正武, 榎本崇宏, 木内陽介 :
紫外線・可視光照射により変動する微生物遺伝子の網羅的解析,
第66回日本細菌学会中国・四国支部総会, 2013年10月. 本庄アイリ, 枝川美幸, 馬渡一諭, 前谷実希, 粟飯原睦美, 下畑隆明, 上番増喬, 上手麻希, 間世田英明, 髙橋章 :
トランスポゾン挿入変異ライブラリを用いた腸炎ビブリオの近紫外線関連遺伝子の探索,
第66回日本細菌学会中国・四国支部総会, 2013年10月. 星山哲平, 芥川正武, 榎本崇宏, 小中信典, 木内陽介, 粟飯原睦美, 林田麻里王, 髙橋章 :
UV照射に対する腸炎ビブリオの遺伝子発現解析,
平成25年度電気関係学会四国支部連合大会講演論文集, 252, 2013年9月.

(要約): 腸炎ビブリオを紫外線に曝露した際，遺伝子発現がどのように変化するかについて，遺伝子チップを用いて網羅的に実験・解析を行っている．UV-A，UV-B，UV-Cそれぞれの代表的な波長について，腸炎ビブリオにUV照射した後に解析を行い，各波長ごとの遺伝子発現の違いについて調査した．

石崎仁愛, 芥川正武, 髙橋章, 木内陽介, 榎本崇宏, 池原敏孝, 小中信典 :
UV-A殺菌推移を表すモデル化に関する研究,
平成25年度電気関係学会四国支部連合大会講演論文集, 245, 2013年9月.

(要約): UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．実験は150uL，0.7L，100Lの各容量のプレートおよびステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた．

Mutsumi Aihara, Takashi Uebanso, Takaaki Shimohata, Kazuaki Mawatari and Akira Takahashi :
Synergistic bactercidal effect of Ultraviolet light with a combination of different,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, Aug. 2013. 林田麻里王, 粟飯原睦美, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章, 星山哲平, 榎本崇宏, 芥川正武, 木内陽介 :
DNA マイクロアレイを用いた微生物遺伝子の網羅的解析,
第247回徳島医学会学術集会, 2013年8月. 常冨愛香里, 粟飯原睦美, 石崎仁愛, 上番増喬, 下畑隆明, 芥川正武, 馬渡一諭, 髙橋章, 正村彰規 :
UVA-LED を用いた水耕栽培用養液の殺菌,
第247回徳島医学会学術集会, 2013年8月. 浅田翔子, 射場仁美, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章 :
腸炎ビブリオの3型分泌装置遺伝子発現は細胞接着により誘導される,
第247回徳島医学会学術集会, 2013年8月. 本庄アイリ, 馬渡一諭, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 :
VP0221とVPA1604は腸炎ビブリオの莢膜多糖類を調節し，抗菌ペプチドと補体耐性に働く,
第247回徳島医学会学術集会, 2013年8月. 根来幸恵, 下畑隆明, 畑山翔, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni 感染によるCl‐輸送チャネルの活性変化,
第247回徳島医学会学術集会, 2013年8月. 畑山翔, 下畑隆明, 根来幸恵, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
食中毒起因菌カンピロバクターによる下痢の機序に関する研究,
第247回徳島医学会学術集会, 2013年8月. 根来幸恵, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni 感染とCl-輸送の変化,
第87回日本感染症学会総会・学術講演会, 2013年6月. 下畑隆明, 根来幸恵, 畑山翔, 松本麻里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染によるクロライド分泌の変化,
第86回日本細菌学会総会, 2013年3月. 浅田翔子, 射場仁美, 下畑隆明, 上番増喬, 馬渡一諭, 髙橋章 :
Vibrio parahaemolyticusの病原因子 TTSS1遺伝子発現は細胞接着によって誘導される,
第65回日本細菌学会・中国・四国支部総会, 2012年10月. 馬渡一諭, 黒川香菜, 本庄アイリ, 藤井麻未, 山本智実, 前谷実希, 粟飯原睦美, 下畑隆明, 上番増喬, 髙橋章 :
Vibrio parahaemolyticusを用いたUVA紫外線照射による殺菌機構の解析,
第65回日本細菌学会・中国・四国支部総会, 2012年10月. 石崎仁愛, 芥川正武, 木内陽介, 榎本崇宏, 小中信典, 髙橋章, 池原敏孝 :
UVAによる殺菌のモデル化に関する研究,
電気関係学会四国支部連合大会講演論文集, 265, 2012年9月.

(要約): UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた．

Tam Thanh Thi Le, Kazuaki Mawatari, Mutsumi Aihara, Takaaki Shimohata, Takashi Uebanso and Akira Takahashi :
VP2118 has major roles in Vibrio parahaemolyticus response to oxidative stress,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, Aug. 2012. 林田沙也加, 下畑隆明, 馬渡一諭, 髙橋章 :
UVA-LEDと塩素を併用した水殺菌法の開発,
第86回日本感染症学会総会・学術講演会, 2012年4月. 山本智実, 馬渡一諭, 林田沙也加, 下畑隆明, 髙橋章 :
発光ダイオードによる近紫外線照射(UVA-LED)によるウイルスの不活化,
第86回日本感染症学会総会・学術講演会, 2012年4月. 馬渡一諭, 井角友香, 平山みなみ, 前谷実希, 山本智実, 林田沙也加, 射場仁美, 粟飯原睦美, 下畑隆明, 髙橋章 :
Vibrio parahaemolyticus capsular polysaccharide is modulated by two pathways, VP0220-1 and VPA1602-4,
第85回日本細菌学会総会, 2012年3月. 下畑隆明, 射場仁美, 根来幸恵, 浅田翔子, 粟飯原睦美, 馬渡一諭, 髙橋章 :
VP1680 induces IL-8 secretion in Vibrio parahaemolyticus infection,
第85回日本細菌学会総会, 2012年3月. 下畑隆明, 射場仁美, 根来幸恵, 浅田翔子, 粟飯原睦美, 馬渡一諭, 髙橋章 :
腸炎ビブリオT3SS1におけるトランスロコンの解析,
第64回日本細菌学会中国・四国支部総会, 2011年10月. 林田沙也加, 粟飯原睦美, 下畑隆明, 馬渡一諭, 芥川正武, 木ノ内陽介, 髙橋章 :
水中の細菌に対するUVA-LEDと塩素との併用殺菌効果,
第64回日本細菌学会中国・四国支部総会, 2011年10月. 真鍋佑輔, 岡久強志, 大和正幸, 髙橋章, 芥川正武, 木内陽介, 榎本崇宏 :
UVA-LED を用いたパイプ型殺菌装置におけるレンズの効果,
電気関係学会四国支部連合大会講演論文集, 233, 2011年9月.

(要約): UV-LEDを用いた新しい殺菌装置の開発を目指し，放射照度分布と殺菌効果の関係を研究している．波長365nmのUVを発光するUVA-LEDを用いてタンク内の水を殺菌する装置を製作した．2種類のタンクを用いて，フレネルレンズを装着した場合と装着していない場合で殺菌実験を行いと殺菌効果への影響を検討した．

真鍋祐矢, 中川亮佑, Su Zehong, 前谷実希, 寺西研二, 下村直行, 髙橋章 :
病原性細菌の遺伝子発現におけるパルス電界の影響に関する研究,
平成23年度電気関係学会四国支部連合大会講演論文集, 238, 2011年9月. 馬渡一諭, Yuka Isumi, Minami Hirayama, Miki Maetani, Tomomi Yamamoto, Sayaka Hayashida, Hitomi Iba, 粟飯原睦美, 下畑隆明, 髙橋章 :
VP0220-0221 and VPA1602-1604 modulate capsular polysaccharides in Vibrio parahaemolyticus,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, 2011年8月. 下畑隆明, 廉馨, 馬渡一諭, 髙橋章 :
腸炎ビブリオ感染は腸管上皮細胞においてMAPKsを活性化しIL-8の分泌を誘発する,
第85回日本感染症学会総会・学術講演会, 2011年4月. 馬渡一諭, 米田由美, 本田真奈美, 射場仁美, 粟飯原睦美, 下畑隆明, 蘇澤紅, 大和正幸, 髙橋章 :
Vibrio parahaemolyticus のチロシンキナーゼVPA1604 は高分子量型莢膜多糖類(HMW-CPS)形成を調節する,
第63回日本細菌学会・中国・四国支部総会, 2010年10月. 粟飯原睦美, 大和正幸, 馬渡一諭, 髙橋章, 芥川正武, 木内陽介 :
異なる紫外線波長の併用による殺菌効果,
第63回日本細菌学会・中国・四国支部総会, 2010年10月. 下畑隆明, 中野政之, 馬渡一諭, 大和正幸, 髙橋章 :
腸炎ビブリオエフェクタータンパク質VP1680 はIL-8 の分泌を誘発する,
第63回日本細菌学会・中国・四国支部総会, 2010年10月. 岡久強志, 真鍋佑輔, 大和正幸, 髙橋章, 芥川正武, 木内陽介 :
UV-LEDを用いたタンク型水殺菌装置,
電気関係学会四国支部連合大会講演論文集, 193, 2010年9月. 大下知洋, 新谷佳子, 片山麻衣, 大和正幸, 芥川正武, 髙橋章, 小中信典, 木内陽介 :
殺菌効果の波長依存性に関する研究,
電気関係学会四国支部連合大会講演論文集, 191, 2010年9月. Kazuaki Mawatari, Yumi Yoneda, Manami Honda, Hitomi Iba, Mutsumi Aihara, Zehong Su, Takaaki Shimohata, Masayuki Yamato and Akira Takahashi :
VPA1604, a protein tyrosine kinase, modulates high molecular weight capsular polysaccharide in Vibrio parahaemolyticus,
日米医学協力研究会コレラ・細菌性腸管感染症専門部会日本側総会, Jul. 2010. 射場仁美, 下畑隆明, 馬渡一諭, 大和正幸, 大西隆仁, 髙橋章 :
腸管ループを用いた腸炎ビブリオ誘発下痢機構の検討,
第64回日本栄養・食糧学会大会, 2010年5月. 下畑隆明, 中野政之, 廉馨, 馬渡一諭, 大和正幸, 髙橋章 :
腸炎ビブリオはMAPKsを介したIL-8の分泌を誘発する,
第64回日本栄養・食糧学会大会, 2010年5月. 中川亮佑, 城山和己, Su Zehong, 寺西研二, 下村直行, 髙橋章 :
病原性細菌へのパルス高電界印加効果,
平成22年電気学会全国大会講演論文集, Vol.1, 201, 2010年3月. Takaaki Shimohata, Masayuki Nakano, Lian Xin, Kazuaki Mawatari, Masayuki Yamato and Akira Takahashi :
Vibrio parahaemolyticus modulate IL-8 secretion through dual pathway,
第83回日本細菌学会総会, Mar. 2010. Kazuaki Mawatari, Yukina Takahashi, Nami Morikawa, Takaaki Shimohata, Akiko Hamamoto, Zehong Su, Masayuki Yamato and Akira Takahashi :
VPA1604, a protein tyrosine kinase, modulate HMW K6-polysaccharide in Vibrio parahaemolyticus,
第83回日本細菌学会総会, Mar. 2010. 中川忠彦, 原田永勝, 吉田将紀, 宮本愛子, 川西由希子, 馬渡一諭, 髙橋章, 阪上浩, 中屋豊 :
脂質合成律速酵素グリセロール-3-リン酸アシルトランスフェラーゼ2の膜トポロジー解析,
第240回徳島医学会学術集会, 2010年2月. 中川亮佑, 城山和己, 山中雅斗, 寺西研二, 下村直行, 髙橋章 :
バイオエレクトリクス実験のためのパルス電界印加システムの開発,
平成21年度電気関係学会四国支部連合大会講演論文集, 235, 2009年9月. 前田未来, 粟飯原睦美, 廉馨, 木内陽介, 芥川正武, 髙橋章 :
UV-LED を用いた野菜の表面殺菌,
電気関係学会四国支部連合大会講演論文集, 236, 2009年9月. 大下知洋, 片山麻衣, 岡久強志, 前田未来, 芥川正武, 木内陽介, 髙橋章, 廉馨 :
異なる波長のUVA-LEDを用いた殺菌作用の比較,
電気関係学会四国支部連合大会講演論文集, 234, 2009年9月. 岡久強志, 木内陽介, 芥川正武, 髙橋章, 前田未来, 大下知洋, 片山麻衣, 廉馨 :
UV-LED を用いた水中殺菌装置の開発,
電気関係学会四国支部連合大会講演論文集, 233, 2009年9月. 芥川正武, 前田未来, 岡久強志, 大下知洋, 中野政之, 髙橋章, 大和正幸, 木内陽介, 廉馨 :
UV-LEDを用いた殺菌装置の開発,
電気学会電子·情報·システム部門大会論文集, No.MC8-8, 829-831, 2009年9月. Qinkai Li, Kazuaki Mawatari, Nagakatsu Harada, 中野政之, Akira Takahashi, Yutaka Nakaya, Toshio Hosaka, Bayasgalan Jambaldorj, 大塚良 and Makoto Funaki :
Chronic 5-Hydroxytryptamine Treatment Induces Insulin Resistance in 3T3-L1 Adipocytes by mTOR-dependent modification of IRS-1,
第238回徳島医学会学術集会, Feb. 2009. 原口さやか, 下畑隆明, 馬渡一諭, 原田永勝, 髙橋章, 髙橋利和, 中尾俊之, 水口潤, 炭谷晴雄, 土井俊夫, 中屋豊 :
慢性腎不全患者における早期の飢餓状態に対する就寝前夜食の効果ー間接カロリーメーターを用いた検討,
第12回日本病態栄養学会学術集会, 2009年1月. Mosrafa Gadelmoula, Xin Lian, 前田未来, Mutsumi Aihara, 八木教行, 片山麻衣, 濱本晶子, Masayuki Yamato, Masatake Akutagawa, Yohsuke Kinouchi, Yutaka Nakaya and Akira Takahashi :
UVA-LEDを用いた空気殺菌の試み,
第23回生体・生理工学シンポジウム, Sep. 2008. 廉馨, 前田未来, Mutsumi Aihara, Akira Takahashi, 八木教行, 橘聡子, 濱本晶子, Masayuki Yamato, Masatake Akutagawa, 中野政之, Yutaka Nakaya and Yohsuke Kinouchi :
UVA-LED装置を用いた野菜の表面殺菌,
第23回生体・生理工学シンポジウム, Sep. 2008. 前田未来, 八木教行, Lian Xin, 木内陽介, 芥川正武, 髙橋章 :
UVA-LED を用いた野菜の表面殺菌,
電気関係学会四国支部連合大会講演論文集, 237, 2008年9月. 八木教行, 濱本晶子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 大和正幸, 木内陽介 :
UV-LEDを用いたタンク水の殺菌効果の検証,
電気関係学会四国支部連合大会講演論文集, 238, 2008年9月. Gadelmoula Mosrafa, Lian Xin, Maeda Miku, Aihara Mutsumi, Hamamoto Akiko, Masayuki Yamato, Yutaka Nakaya, Yohsuke Kinouchi and Akira Takahashi :
高出力UVA-LEDを用いた空気殺菌システムの開発,
日本防菌防黴学会第35回年次大会, Sep. 2008. 粟飯原睦美, 廉馨, 前田未来, 橘聡子, 大和正幸, 中野政之, 芥川正武, 木内陽介, 髙橋章, 中屋豊 :
高出力紫外線装置を用いた野菜表面の殺菌,
日本防菌防黴学会第35回年次大会, 2008年8月. 八木教行, 森美怜, 濱本晶子, 中野政之, 芥川正武, 橘聡子, 髙橋章, 木内陽介, 池原敏孝, 大和正幸 :
UVA-LEDを用いたタンク型殺菌装置の開発と殺菌効果の検証,
第47回日本生体医工学会大会, 556-557, 2008年5月. 原口さやか, 下畑隆明, 馬渡一諭, 原田永勝, 保坂利男, 髙橋章, 中尾俊之, 土井俊夫, 中屋豊 :
間接カロリーメーターを用いた，慢性腎不全患者における早朝空腹時の代謝状態の検討,
第62回日本栄養・食糧学会, 2008年5月. Lian Xin, Akira Takahashi, Maeda Miku, Yagi Noriyuki, Tachibana Satoko, Masayuki Yamato, Tetsuro Koga, Masatake Akutagawa, Nakano Masayuki, Yutaka Nakaya and Yohsuke Kinouchi :
New surface sterilization system using UVA-LED for vegetables,
第82回日本感染症学会総会・学術講演会, Apr. 2008. 八木教行, 森美怜, 濱本晶子, 橘聡子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
UV-LEDを用いたタンク水の殺菌,
電気関係学会四国支部連合大会講演論文集, 232, 2007年9月. 濱本晶子, 中野政之, 八木教行, 芥川正武, 橘聡子, 池原敏孝, 髙橋章, 木内陽介, 中屋豊 :
高出力紫外線発光ダイオードを用いた殺菌,
日本防菌防黴学会第34回年次大会, 2007年8月. 八木教行, 森美玲, 濱本晶子, 橘聡子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
UV-LEDを用いたタンク水の殺菌,
MEとバイオサイバネティックス研究会(MBE), 2007年7月. 濱本晶子, 中野政之, 髙橋章 :
近紫外線発光ダイオードを用いた殺菌システム,
第81回日本感染症学会総会, 2007年4月. 髙橋章, 中野政之 :
ナグビブリオが産生するエルトールヘモリシンの下痢誘発機構,
第81回日本感染症学会総会, 2007年4月. 濱本晶子, 髙橋章, 森美怜, 中野政之, 池原敏孝, 木内陽介 :
高出力紫外線発光ダイオードを用いた殺菌装置の開発,
日本細菌学雑誌「第80回日本細菌学会総会」, Vol.62, No.1, 130, 2007年3月. 森美怜, 濱本晶子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
紫外発光LEDによる殺菌,
第21回生体・生理工学シンポジウム論文集, 547-548, 2006年11月. 森美怜, 濱本晶子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
紫外発光LEDの病原性菌への影響,
電気関係学会四国支部連合大会講演論文集, 196, 2006年9月. 中屋豊, 安井苑子, 馬渡一諭, 髙橋章, 原田永勝, Hossein Nazari, 河野崇, 大下修造, 古川哲史 :
機能性食品の心血管系イオンチャネルに対する作用-Phytoestrogenのgenomicおよびnongenomic action,
長井長義記念シンポジウム, 2006年9月. 森美怜, 濱本晶子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
紫外発光LEDを用いた殺菌,
平成18年度(第39回)照明学会全国大会講演論文集, 245, 2006年8月. 髙橋章 :
New water sterilization system used by UVA-LED,
日米コレラ部会日本側総会, 2006年8月. 森島真幸, 中屋豊, 髙橋章, 磯本正二郎, 小野克重 :
自発的高運動性ラットSPORTSの海馬ノルエピネフリン神経伝達と血圧制御の異常．,
第5回九州脳と高血圧研究会, 2006年7月. 森美怜, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
紫外発光LEDを用いた殺菌作用に関する研究,
電気関係学会四国支部連合大会講演論文集, 225, 2005年9月. 森島真幸, 原小由合, 原田永勝, 佐野敦子, 妹尾廣正, 髙橋章, 中屋豊 :
高運動性モデルラットSPORTSにおける海馬ノルエピネフリン動態は運動習性を調節する.,
第82回日本生理学会, 2005年5月. 森島真幸, 原田永勝, 原小由合, 佐野敦子, 妹尾廣正, 髙橋章, 中屋豊 :
高運動性モデルラットSPORTSにおける運動習性と脳内モノアミン制御.,
第59回日本栄養食糧学会, 2005年5月. 中野政之, 児玉年央, 髙橋章 :
ノルエピネフリンによる腸炎ビブリオの病原性に対する影響,
第78回日本細菌学会総会, 2005年4月. 髙橋章, 中野政之, 中屋豊 :
腸管内細菌感染による腸管上皮細胞のクロライド分泌変化,
第1回日本消化管学会, 2005年1月. 清家卓也, 中西秀樹, 松本和也, 川上慎吾, 森山啓司, 髙橋章 :
顎裂骨移植の評価と予後予測-裂の形態と骨塩量について-,
第45回日本形成外科学会総会・学術集会, 2002年4月. 三木真理, 中村陽一, 日野弘之, 大串文隆, 髙橋章, 吉永純子, 前澤博, 安岡劭 :
ヒト気道トリプシン様プロテアーゼ(HAT)の気道細胞内遊離Ca²⁺濃度[Ca²⁺]に対する作用,
第42回日本呼吸器学会総会プログラム, 112, 2002年4月. 細川敬子, 山口久雄, 池原敏孝, 髙橋章, 吉﨑和男 :
低K+条件下での細胞周期調節因子の発現量とその局在,
第74回日本生理学会大会, 1997年3月. 池原敏孝, 山口久雄, 細川敬子, 髙橋章, 宮本博司, 吉﨑和男 :
HeLa細胞の利尿剤感受性K+排出とRb+取込みに及ぼす細胞内外イオンの影響,
第73回日本生理学会大会, 1996年4月. 細川敬子, 山口久雄, 池原敏孝, 髙橋章, 吉﨑和男, 宮本博司 :
同調した細胞の細胞周期調節蛋白の発現とKイオンの役割,
第73回日本生理学会大会, 1996年4月. 細川敬子, 山口久雄, 池原敏孝, 髙橋章, 吉﨑和男 :
低K+条件下におけるHeLa細胞の細胞周期調節因子の発現,
第72回日本生理学会大会, 1995年3月. 池原敏孝, 山口久雄, 細川敬子, 髙橋章, 吉﨑和男, 宮本博司 :
培養HeLa細胞における利尿剤感受性外向きと内向きK+輸送,
第72回日本生理学会大会, 1995年3月.

研究会・報告書

中山知彦, 梅原英裕, 富岡有紀子, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 髙橋章, 岡本泰昌, 沼田周助 :
双極性障害における血中メタボローム解析,
第42回躁うつ病の薬理・生化学的研究懇話会, 2023年10月. 石田快, 下畑隆明, 神田結奈, 増田瑠見子, 山﨑浩平, 上番増喬, 馬渡一諭, 柏本孝茂, 髙橋章 :
Vibrio vulnificus創傷感染が引き起こす宿主骨格筋内代謝変化の解析,
第16回細菌学若手コロッセウム, 2022年8月. 中山知彦, 梅原英裕, 富岡有紀子, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 髙橋章, 岡本泰昌, 沼田周助 :
双極性障害における血中代謝物の変化,
第13回脳科学クラスターミニリトリート, 2022年2月. 原口雅宣, 永瀬雅夫, 安井武史, 木内陽介, 宮脇克行, 髙橋章, 岡久稔也 :
2021年度におけるLEDライフイノベーション総合プラットフォーム推進事業の取り組み,
LED総合フォーラム2022 in 徳島, P-1, 2022年1月. 中山知彦, 梅原英裕, 富岡有紀子, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 沼田周助, 髙橋章, 岡本泰昌, 大森哲郎 :
うつ病におけるキヌレニン経路の変化,
第40回躁うつ病の薬理・生化学的研究懇話会, 2021年10月. 中山知彦, 梅原英裕, 富岡有紀子, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 沼田周助, 髙橋章, 岡本泰昌, 大森哲郎 :
うつ病におけるキヌレニン経路の変化,
第12回脳科学クラスターミニリトリート, 2021年2月. 原口雅宣, 北田貴弘, 永瀬雅夫, 安井武史, 木内陽介, 宮脇克行, 髙橋章, 岡久稔也 :
2020年度におけるLEDライフイノベーション総合プラットフォーム推進事業の取り組み,
LED総合フォーラム2021 in 徳島, P-1, 2021年2月. 中山知彦, 梅原英裕, 富岡有紀子, 上敷領俊晴, 淵上学, 岡田剛, 増田瑠見子, 馬渡一諭, 中瀧理仁, 沼田周助, 髙橋章, 岡本泰昌, 大森哲郎 :
うつ病におけるキヌレニン経路の変化,
第5回メタボロームシンポジウム, 2020年12月. 池原敏孝, 中橋睦美, 土屋浩一郎, 榎本崇宏, 芥川正武, 髙橋章, 木内陽介 :
405 nm LED光照射に誘導される活性酸素が培養HeLaS3細胞に及ぼす影響,
LED総合フォーラム2020 in 徳島, 145-148, 2020年2月. 木内陽介, 原口雅宣, 髙橋章, 岡久稔也 :
徳島大学ライフオプティクス研究プロジェクト,
LED総合フォーラム2020in徳島, 73-74, 2020年2月.

(キーワード): ライフオプティクス / LED / LEDバレイ徳島

下畑隆明, 木戸純子, 鳴滝涼香, 佐藤優里, 扶川留音, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染による，上皮細胞のアミノ酸輸送変動に関する検討,
第12回日本カンピロバクター研究会総会, 2019年9月. 福島志帆, 下畑隆明, 木戸純子, 辻口舞, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染においてオートファジーは菌の侵入過程を促進する,
第12回日本カンピロバクター研究会総会, 2019年9月. 辻口舞, 下畑隆明, 木戸純子, 福島志帆, 石田快, 吉本亜由美, 上番増喬, 馬渡一諭, 髙橋章 :
抗菌薬投与マウスモデルを用いたCampylobacter jejuniの感染によるコレステロール代謝への影響,
第12回日本カンピロバクター研究会総会, 2019年9月. 原口雅宣, 北田貴弘, 永瀬雅夫, 安井武史, 木内陽介, 宮脇克行, 髙橋章, 岡久稔也 :
LEDライフイノベーション総合プラットフォーム推進事業の取り組み,
LED総合フォーラム2019in徳島, P-2, 2019年2月. 木内陽介, 原口雅宣, 髙橋章, 岡久稔也 :
徳島大学ライフオプティクス研究プロジェクトの進展,
LED総合フォーラム2019in徳島, P-1, 2019年2月. 畑山翔, 下畑隆明, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 中本晶子, 首藤恵泉, 上番増喬, 馬渡一諭, 髙橋章 :
UVA-LED照射による調理器具汚染Campylobacter jejuniの殺菌効果について,
第11回日本カンピロバクター研究会総会, 2018年12月. 石田快, 下畑隆明, 畑山翔, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの上皮細胞内生存とコレステロールの関連,
第11回日本カンピロバクター研究会総会, 2018年12月. 辻口舞, 下畑隆明, 福島志帆, 畑山翔, 木戸純子, 石田快, 上番増喬, 馬渡一諭, 髙橋章 :
Microarrayの解析から導くCampylobacter jejuniの侵入による宿主腸管上皮細胞での脂質代謝への影響,
第11回日本カンピロバクター研究会総会, 2018年12月. 二川健, 髙橋章, 宮脇克行, 出口祥啓 :
無重力や寝たきりによる筋萎縮の分子メカニズムとその栄養学的アプローチ,
ナノオプティクス研究グループ第25回研究討論会, 2018年11月. 天宅あや, 下畑隆明, 畑山翔, NGUYEN QUOC ANH, 木戸純子, 福島志帆, 石田快, 上番増喬, 馬渡一諭, 髙橋章 :
宿主細胞における小胞体ストレス応答はCampylobacter jejuniの細胞内侵入を抑制する,
第257回徳島医学会学術集会, 2018年8月. 原口雅宣, 北田貴弘, 永瀬雅夫, 安井武史, 木内陽介, 宮脇克行, 髙橋章, 岡久稔也 :
LEDライフイノベーション総合プラットフォーム推進事業におけるテラヘルツLED応用基盤技術に関する取り組み,
LED総合フォーラム2018in徳島, P-2, 2018年2月. 常冨愛香里, 畑山翔, 下畑隆明, 木戸純子, 天野幸恵, 中橋睦美, 上番増喬, 馬渡一諭, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
Campylobacter jejuni汚染対策に向けたUVA-LEDの有用性について,
第10回日本カンピロバクター研究会総会, 2017年12月. 福島志帆, 下畑隆明, 畑山翔, 木戸純子, 天宅あや, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniが誘導するオートファジーが菌の侵入および生存に与える影響について,
第10回日本カンピロバクター研究会総会, 2017年12月. 馬渡一諭, 渡邊瞳, 西坂理沙, 中橋睦美, 常冨愛香里, 児島瑞基, 野上夏希, 牧野美鈴, 増田瑠見子, 下畑隆明, 上番増喬, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
透析液の細菌汚染調査と近紫外発光ダイオードによる殺菌効果の評価,
電子情報通信学会技術研究報告(MEとバイオサイバネティックス), Vol.117, No.165, 13-16, 2017年7月.

(キーワード): 人工透析 / 細菌汚染 / 近紫外線 / 発光ダイオード (LED) / 殺菌

原口雅宣, 木内陽介, 北田貴弘, 永瀬雅夫, 安井武史, 宮脇克行, 髙橋章, 岡久稔也 :
LEDライフイノベーション総合プラットフォーム推進事業におけるテラヘルツLED応用基盤技術に関する取り組み,
LED総合フォーラム2016in徳島論文集, 201-202, 2016年12月. 畑山翔, 下畑隆明, 天野幸恵, 木戸純子, 神田結奈, 天宅あや, 福島志帆, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniの宿主腸管上皮細胞への侵入とTight Junctions形成の関連について,
第9回日本カンピロバクター研究会総会, 2016年11月. 木戸純子, 下畑隆明, 佐藤優里, 畑山翔, 神田結奈, 天宅あや, 福島志帆, 上番増喬, 馬渡一諭, 髙橋章 :
宿主アミノ酸輸送の変化と宿主細胞内Campylobacter jejuni生存について,
第9回日本カンピロバクター研究会総会, 2016年11月. 天宅あや, 下畑隆明, 畑山翔, 木戸純子, 上番増喬, 馬渡一諭, 原田永勝, 髙橋章 :
Campylobacter jejuni感染で誘導される小胞体ストレスについて,
第9回日本カンピロバクター研究会総会, 2016年11月. 佐藤優里, 下畑隆明, 扶川留音, 根来幸恵, 畑山翔, 木戸純子, 上番増喬, 原田永勝, 馬渡一諭, 髙橋章 :
メタボローム解析を用いた宿主細胞内のCampylobacter jejuniのエネルギー源獲得機構の検索,
第8回日本カンピロバクター研究会総会, 2015年12月. 常冨愛香里, 下畑隆明, 後藤茉凜, 天野幸恵, 中橋睦美, 上番増喬, 馬渡一諭, 宮脇克行, 榎本崇宏, 芥川正武, 木内陽介, 髙橋章 :
Campylobacter jejuniに対するUVA-LEDの有効性,
第8回日本カンピロバクター研究会総会, 2015年12月. 木戸純子, 下畑隆明, 根来幸恵, 畑山翔, 佐藤優里, 扶川留音, 吉兼道子, 上番増喬, 馬渡一諭, 髙橋章 :
CFTR発現によるCampylobacter jejuniの上皮細胞侵入抑制機構について,
第8回日本カンピロバクター研究会総会, 2015年12月. 下畑隆明, 木戸純子, 根来幸恵, 畑山翔, 佐藤優里, 中橋睦美, 上番増喬, 馬渡一諭, 髙橋章 :
CFTRはCampylobacter jejuniの侵入抑制に寄与する,
第7回日本カンピロバクター研究会総会, 2014年12月. 佐藤優里, 下畑隆明, 根来幸恵, 畑山翔, 木戸純子, 中橋睦美, 上番増喬, 原田永勝, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染とAutophagyの関連について,
第7回日本カンピロバクター研究会総会, 2014年12月. 根来幸恵, 下畑隆明, 畑山翔, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuni感染はCFTR機能を抑制する,
第6回日本カンピロバクター研究会, 2013年7月. 畑山翔, 下畑隆明, 根来幸恵, 松本麻里, 佐藤優里, 粟飯原睦美, 上番増喬, 馬渡一諭, 髙橋章 :
Campylobacter jejuniによる下痢誘発機構の検討,
第6回日本カンピロバクター研究会, 2013年7月. 石崎仁愛, 真鍋佑輔, 芥川正武, 木内陽介, 池原敏孝, 小中信典, 髙橋章, 榎本崇宏 :
UV-A(近紫外線)照射の殺菌効果に関するモデル化,
LED総合フォーラム2012in徳島論文集, 79-80, 2012年4月.

(要約): UV-Aを用いた殺菌について，UV-A照射量と菌数の関係を表すモデル式を提案し，実験結果と比較している．0.7Lステンレスタンクについて，大腸菌を一定濃度で入れた菌液を用いて近紫外線を照射して菌数の時間的推移を測定した．その結果，菌数の時間推移の傾向をモデル式で表すことができた．

真鍋佑輔, 芥川正武, 木内陽介, 池原敏孝, 髙橋章, 小中信典, 榎本崇宏, 石崎仁愛 :
近紫外線が細菌に与える影響に関する研究,
LED総合フォーラム2012in徳島論文集, 77-78, 2012年4月.

(要約): 波長365nmのUVA-LEDを用いた水殺菌装置について検討した．殺菌容器は内径22mmから56mm，深さ50mmから150mmのステンレス製の円筒容器を製作し，上部からUV-LEDを照射する構造とした．またLEDから照射されたUVを有効に利用するためにフレネルレンズを用いた．非病原性大腸菌を用いた実験で殺菌効果を確認した．

大下知洋, 岡久強志, 真鍋佑輔, 大和正幸, 榎本崇宏, 芥川正武, 髙橋章, 木内陽介 :
光殺菌効果の波長依存性に関する研究,
LED総合フォーラム2011in徳島論文集, 89-90, 2011年6月. Mostafa Gadelmoula, Xin Lian, Akiko Hamamoto, Kazuaki Mawatari, Masayuki Yamato, Masatake Akutagawa, Yutaka Nakaya, Yohsuke Kinouchi and Akira Takahashi :
UVA-LED suitability for air disinfection,
LED総合フォーラム論文集, 85-86, Apr. 2010. Xin Lian, Miku Maeda, Masayuki Yamato, Kazuaki Mawatari, Masatake Akutagawa, Yutaka Nakaya, Yohsuke Kinouchi and Akira Takahashi :
New UVA-LED surface sterilization system for vegetables,
LED総合フォーラム論文集, 83-84, Apr. 2010. 岡久強志, 眞鍋佑輔, 大和正幸, 髙橋章, 芥川正武, 木内陽介 :
UVA-LED装置を用いた水殺菌装置の開発,
LED総合フォーラム論文集, 81-82, 2010年4月. 粟飯原睦美, 新谷佳子, 廉馨, Mostafa Gadelmoula, 大和正幸, 馬渡一諭, 芥川正武, 木内陽介, 髙橋章 :
UVA-LED装置を用いた水殺菌装置の開発,
LED総合フォーラム論文集, 79-80, 2010年4月. 大和正幸, 料治春華, 髙橋章, 芥川正武, 木内陽介 :
UVA LEDによる水殺菌, --- タンク容量と循環速度の違いによる殺菌効率の検討 ---,
LED総合フォーラム論文集, 77-78, 2010年4月. 森美怜, 濱本晶子, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
紫外発光LEDの細菌への影響,
電子情報通信学会技術研究報告, Vol.106, No.162, 5-8, 2006年7月. 森美怜, 中野政之, 芥川正武, 髙橋章, 池原敏孝, 木内陽介 :
紫外発光LEDの大腸菌への影響,
電子情報通信学会技術研究報告, Vol.105, No.222, 25-28, 2005年7月. 植野美彦, 関陽介, 内海千種, 岩佐武, 髙橋章, 安井敏之, 川人伸次, 尾崎和美, 藤野裕道, 髙栁俊夫, 服部武文, 齊藤隆仁, 上岡麻衣子 :
令和5年度徳島大学高等教育研究センターアドミッション部門報告書,
令和5年度徳島大学高等教育研究センターアドミッション部門報告書, 2024年3月. 植野美彦, 関陽介, 衣川仁, 森岡久尚, 髙橋章, 森健治, 石丸直澄, 尾崎和美, 山﨑哲男, 高田篤, 宇都義浩, 齊藤隆仁, 上岡麻衣子 :
令和4年度徳島大学高等教育研究センターアドミッション部門報告書,
令和4年度徳島大学高等教育研究センターアドミッション部門報告書, 2023年3月.

特許: 三好仁志, 髙橋章, 馬渡一諭, 福島志帆, 粟飯原睦美, 宮脇克行, 二川健, 牧野美鈴 : (2023年3月), 特許第2023-046795号. 木内陽介, 池原敏孝, 髙橋章, 芥川正武, 中野政之, 森美怜, 有田憲一 : 紫外線殺菌装置, 特願2005-190625 (2005年6月), 特開2007-7083 (2007年1月), 特許第4771402号 (2011年7月).
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 非伝搬性のハイブリッド光を用いた環境や人にやさしい殺菌システム (研究課題/領域番号: 23K18578 )
便秘の客観的評価のための腸音解析システムの開発 (研究課題/領域番号: 20K12755 )
カンピロバクターの鶏肉表面汚染低減を目指した、LED光殺菌システムの開発 (研究課題/領域番号: 20K11647 )
ケトン体代謝の流れがエピゲノム修飾と細胞機能、代謝疾患発症に及ぼす影響の解明 (研究課題/領域番号: 20K11624 )
光環境制御による住空間衛生管理の新機軸 (研究課題/領域番号: 20H01616 )
腸内細菌叢の活性で調節される食物由来の概日リズム振動化合物の同定及びヒトへの応用 (研究課題/領域番号: 18KK0274 )
生体内・外の代謝情報を統合したハイブリッド栄養学の創出 (研究課題/領域番号: 18K19746 )
全身麻酔による脳内神経炎症機構の解明 -高齢者に最適な麻酔法の確立をめざして- (研究課題/領域番号: 18H02898 )
小児先天性胆道拡張症における胆道癌発癌機構解明に関する研究 (研究課題/領域番号: 17K11510 )
バクテリアルトランスロケーション下の腸管免疫機構の解明と治療法への展開 (研究課題/領域番号: 25461950 )
腸炎ビブリオにおけるIII型分泌機能の数と病原性誘導 (研究課題/領域番号: 24115516 )
近紫外線を用いた殺菌効果の波長依存性と機序の解明 (研究課題/領域番号: 23500519 )
バクテリアルトランスロケーション下のタイトジャンクション傷害と治療への展開 (研究課題/領域番号: 22591489 )
カテコールアミンによる病原性細菌の病原性変化 (研究課題/領域番号: 22590393 )
バクテリアルトランスロケーションの新たなメカニズムの解明 (研究課題/領域番号: 21791293 )
バクテリアルトランスロケーションのパラセルラールートの解析と重症度評価法の開発 (研究課題/領域番号: 19591485 )
神経細胞の生理機能におよぼす通信領域の高周波電磁波の影響 (研究課題/領域番号: 19510032 )
新しいバクテリアルトランスロケーションのモニタリング法ならびに予防法の開発 (研究課題/領域番号: 17591338 )
腸炎ビブリオ病原因子産生の特異的翻訳調節機構による制御 (研究課題/領域番号: 17590393 )
腸炎ビブリオ感染による腸管運動の変化が下痢誘発に与える影響 (研究課題/領域番号: 14770117 )
TLRとMox1オキシダーゼを介する消化管粘膜の自然免疫応答と発がんの分子基盤 (研究課題/領域番号: 14370184 )
腸炎ビブリオの下痢誘発機序の解明 (研究課題/領域番号: 12770133 )
研究者番号（90304047）による検索

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専門分野・研究分野: 栄養学 (Nutrition)
生理学 (Physiology)
細菌感染症学 (Bacterial Infectious Diseases)
所属学会・所属協会: 日本栄養·食糧学会
日本生理学会
日本細菌学会
日本体力医学会
日本環境感染症学会
American society for microbiology
日本感染症学会
委員歴・役員歴: 日本細菌学会 (評議委員)
日本体力医学会 (評議員)
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2024年11月16日更新

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Jグローバル最終確認日: 2024/11/16 01:04
氏名（漢字）: 高橋章
氏名（フリガナ）: タカハシアキラ
氏名（英字）: Takahashi Akira
所属機関: 徳島大学

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researchmap最終確認日: 2024/11/17 01:38
氏名（漢字）: 高橋章
氏名（フリガナ）: タカハシアキラ
氏名（英字）: Akira Takahashi
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登録日時: 2019/11/5 15:46
更新日時: 2024/9/29 11:14
アバター画像URI: https://researchmap.jp/6339428/avatar.jpg
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所属ID: 0344000000
所属: 徳島大学
部署: 大学院医歯薬学研究部予防環境栄養学分野
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学位: 医学博士
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研究者番号: 90304047

所属（現在）: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 教授

所属（過去の研究課題 情報に基づく）*注記: 2018/4/1 – 2023/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 教授
2017/4/1 : 徳島大学, 大学院医歯薬学研究部(医学系), 教授
2014/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2011/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 教授
2012/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 特任教授
2010/4/1 – 2012/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2008/4/1 : 徳島大学, ヘルスバイオサイエンスル研究部, 教授
2008/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2007/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授
2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教授
2004/4/1 – 2005/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教授
2003/4/1 : 徳島大学, 医学部, 助教授
2000/4/1 – 2002/4/1 : 徳島大学, 医学部, 講師

審査区分/研究分野

研究代表者

医学 / 病理 / 細菌学(含真菌学)
生物系 / 医歯薬学 / 基礎医学 / 細菌学(含真菌学)
生物系
中区分59:スポーツ科学、体育、健康科学およびその関連分野
小区分08030:家政学および生活科学関連
中区分90:人間医工学およびその関連分野

研究代表者以外

総合・新領域系 / 複合新領域 / 環境学 / 環境影響評価・環境政策
生物系 / 医歯薬学 / 外科系臨床医学 / 外科学一般
生物系 / 医歯薬学 / 外科系臨床医学 / 消化器外科学
総合・新領域系 / 総合領域 / 人間医工学 / 医用生体工学・生体材料学
医学 / 内科 / 消化器内科学
生物系 / 医歯薬学 / 外科系臨床医学 / 小児外科学
中区分59:スポーツ科学、体育、健康科学およびその関連分野
小区分55050:麻酔科学関連
小区分59040:栄養学および健康科学関連
小区分90150:医療福祉工学関連

キーワード

研究代表者

腸炎ビブリオ / TDM / TRH / Ca^<2+>依存性Cl^-チャネル / アポトーシス / Vibrio parahaemolyticus / TDH / Cl-secretion / Ca2+ / 耐熱性溶血毒 / 細胞内Ca2+ / protein kinase C / PKC / Ca^<2+> / カテコールアミン / 病原性 / ノルエピネフリン / 病原性活性化機構 / 一分子 / 細菌 / 翻訳 / small RNA / 翻訳後調節 / 病原因子 / translation / post transcriptional regulation / virulence factor / 腸内微生物細菌叢 / 概日リズム / 肝臓 / 中性脂肪 / Gut microbiome / 住空間 / 光環境 / ナローバンド / 微生物 / 殺菌 / 光 / 非伝搬性

研究代表者以外

F-actin 変動磁界 / 高周波電磁界 / クロマフィン細胞 / 細胞内Ca^<2+> / コラーゲン合成 / アセチルコリン / 小胞体 / F-actin / 変動磁界 / 電磁波 / 438.5MHz / 細胞内カルシウム濃度 / 低浸透圧 / アクチン蛋白質 / 1.5テスラ / 細胞容積調節機構 / バクテリアルトランスロケーション / systemic inflammatory response syndrome(SIRS) / 敗血症 / タイトジャンクション / パラセルラールート / SIRS / Trans Epithelial Resistance(TER) / Rabファミリー低分子量G蛋白質 / CPT-11 / 5FU / オーファジー / 腸管傷害 / オートファジー / Toll like receptor / 腸上皮セルライン / オートファゴゾーム / トールライクレセプター / 腸内細菌叢 / CPT11 / TNFα / NF-κB / NFκB / 近紫外線 / 遺伝子発現 / 腸炎ビブリオ / 殺菌 / 遺伝子発現解析 / 波長依存性 / 遺伝子解析 / Toll Like Receptor (TLR) / PD-1 / TFH / CD4+Foxp3+Treg / IL10 / IgA / Toll様受容体 / NADPH oxidase 1 / 消化管粘膜上皮細胞 / 活性酸素 / 自然免疫応答 / 発がん / ヘリコバクタ・ピロリ菌 / フラジェリン / 活性酵素 / Toll-like receptor / gastrointestinal epithelium / reactive oxygen species / innate immunity / carcinogenesis / Helicobacter pylori / flagellin / 感染症 / 外科 / ストレス / 免疫学 / バクテリアルトランスロケ / サイトカイン / Infection / Surgery / Stress / Immunology / Bacterial translocation / 先天性胆道拡張症 / 小児 / 胆道癌 / 発癌 / メタボローム解析 / 腸内菌叢 / ビタミンB / 栄養学 / 術後せん妄 / 脳内神経炎症 / 術後認知機能障害 / ミクログリア / 術後神経認知異常 / ケトン体 / 代謝 / 栄養 / 生活習慣病 / 代謝の流れ / UVA / カンピロバクター / LED / 塩素 / 鶏肉 / 腸音 / 便秘 / 音響特徴量

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近紫外-深紫外発光ダイオード照射による病原ウイルス不活化の最適化シミュレーション技術の開発と応用

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