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苛原稔

徳島大学

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2024年4月16日更新

職名: 名誉教授 (2022.4)
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学歴: 1979/3: 徳島大学医学部医学科卒業
1983/3: 徳島大学大学院医学研究科修了
学位: 医学博士 (徳島大学) (58年11月)
職歴・経歴: 1983/4: 徳島大学医学部附属病院医員
1983/8: 徳島大学医学部附属病院助手
1992/10: 徳島大学医学部附属病院講師
2001/4: 徳島大学医学部産科婦人科学助教授
2001/7: 徳島大学医学部産科婦人科学教授
2003/10: 徳島大学医学部·歯学部附属病院副院長

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2024年4月16日更新

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研究テーマ: 視床下部·下垂体の内分泌機構に関する研究 (視床下部, 下垂体) (各種摂食調節物質の視床下部·下垂体からのゴナドトロピン分泌機構に対する影響の検討)
卵胞発育と閉鎖の機序に関する研究 (卵胞発育機構) (各種サイトカインの発育と閉鎖の機序に関する検討)
排卵障害の発症機構と治療法に関する研究 (卵巣障害) (多嚢胞性卵巣症候群の病因·病態に関すす検討)
単一排卵誘発法の開発 (排卵誘発法)

著書

苛原稔, 竹内麻由美 :
一冊でわかる婦人科腫瘍の画像診断, --- 子宮腺筋症 ---,
文光堂, 東京, 2019年9月. 苛原稔, 竹内麻由美 :
一冊でわかる婦人科腫瘍の画像診断, --- 子宮筋腫 ---,
文光堂, 東京, 2019年9月. 苛原稔 :
14.不妊症ってなに? ， 29.女性が産みやすい社会へ,
毎日新聞出版, 2018年2月. 苛原稔 :
38.内分泌・代謝系女性ホルモン剤，39. 内分泌・代謝系その他のホルモン剤-排卵誘発治療について,
2018年1月. 加藤剛志, 苛原稔 :
乳腺症・乳管内乳頭種,
日本医事新報社, 2017年7月. 苛原稔 :
第1章基礎知識D視床下部ホルモン，4.GnRH,
株式会社診断と治療社, 2016年11月. 前田和寿, 苛原稔 :
3.合併症妊娠 ②呼吸器疾患(気管支喘息),
株式会社メジカルビュー社, 2016年7月. 苛原稔 :
内分泌・代謝系 38.女性ホルモン剤，39.その他のホルモン剤-排卵誘発治療について-,
株式会社じほう, 2016年1月. 苛原稔 :
資料「日本産科婦人科学会会告」，「日本生殖医学会理事会·倫理委員会の報告および会告」,
医薬ジャーナル社, 2016年1月. 苛原稔 :
Ⅱ月経周期と妊娠の成立機構,
医薬ジャーナル社, 2016年1月. 苛原稔 :
Ⅰ女性の年齢と妊娠·不妊について,
医薬ジャーナル社, 2016年1月. 松崎利也, 松井寿美佳, 岩佐武, 苛原稔 :
月経不順と女性医療,
フジメディカル出版, 2014年11月. 苛原稔 :
高プロラクチン血症に対する診断・治療，一般不妊治療における多胎妊娠の予防,
日本生殖医学会, 2014年10月. 岩佐武, 松崎利也, 苛原稔 :
体重減少性月経および神経性食欲不振症の診断と治療,
株式会社中外医学社, 2014年7月. 吉本勝彦, 赤池雅史, 苛原稔, 青田桂子 :
歯科医師のための医学ハンドブック,
医歯薬出版株式会社, 東京, 2014年5月. 苛原稔 :
「産婦人科疾患 -卵巣過剰刺激症候群- 」,
2014年1月. 松井寿美佳, 松崎利也, 苛原稔 :
第1章，4.肥満，A.肥満と不妊症・PCOS,
株式会社金芳堂, 2013年6月. 苛原稔 :
1. 月経異常，b.多嚢胞性卵巣症候群(PCOS),
南江堂, 2013年5月. 苛原稔 :
1.月経異常，b.多囊胞性卵巣症候群(PCOS),
南江堂, 2013年5月. 苛原稔 :
婦人科乳腺外科,
メディックメディア, 2013年3月. 前田和寿, 前川正彦, 苛原稔 :
風疹抗体,
文光堂, 2013年3月. 前田和寿, 苛原稔 :
呼吸器疾患(器官支喘息),
株式会社メジカルビュー社, 2012年7月. 苛原稔 :
LH/FSH,
株式会社診断と治療社, 2012年4月. 苛原稔 :
GnRH,
株式会社診断と治療社, 2012年4月. 森崇英, 麻生武志, 石塚文平, 苛原稔, 岡村均, 久保春海, 香山浩二, 小西郁生, 佐々木裕之, 佐藤英明, 野瀬俊明, 柴原浩章, 島田昌之, 角田幸雄 :
卵子学,
京都大学学術出版会, 2011年9月. 山本由理, 加藤剛志, 炬口恵理, 吉田加奈子, 田中優, 加地剛, 山本哲史, 桑原章, 苛原稔 :
抗NMDAR(N-methy-D-aspartate receptor)脳炎をきたした卵巣成熟奇形腫の一例,
2010年9月. 松崎利也, 岩佐武, 木内理世, 藤澤しのぶ, 苛原稔 :
プロラクチン,
株式会社診断と治療社, 2010年4月. 前川正彦, 苛原稔 :
臨床検査ガイド 2005∼2006, --- 風疹抗体検査 ---,
文光堂, 東京, 2005年2月. 安井敏之, 苛原稔 :
Annual Review 内分泌，代謝 2004, --- プロラクチン ---,
株式会社中外医学社, 東京, 2004年1月.

(キーワード): prolactin / prolactin receptor / prolactin secretion

安井敏之, 苛原稔 :
Annual Review 内分泌，代謝 2003, --- プロラクチン ---,
株式会社中外医学社, 東京, 2003年1月.

(キーワード): prolactin / prolactin receptor / prolactin secretion / pituitary

安井敏之, 苛原稔, 青野敏博 :
少産少子と今後の課題少産少子の医学的側面,
株式会社中山書店, 東京, 2001年8月. 上村浩一, 手束典子, 山田正代, 安井敏之, 苛原稔, 青野敏博 :
やさしい更年期障害の自己管理,
医薬ジャーナル社, 大阪, 2000年12月. 安井敏之, 苛原稔, 青野敏博 :
排卵障害治療の実際漢方療法,
株式会社中山書店, 東京, 2000年1月. 安井敏之, 苛原稔, 青野敏博 :
The Management of the Menopause:The Millennium Review 2000,
The Parthenon publishing Group, ロンドン, 1999年10月.

論文

藤川理恵, 高開登茂子, 久米博子, 三木幸代, 原田路可, 加根千賀子, 赤池雅史, 楊河宏章, 苛原稔 :
モンゴル国 A 病院のモンゴル人看護師のリスク感性の分析,
日本看護学会誌 18(1): 13-20, 2023, Vol.18, No.1, 13-20, 2023年. Yohei Takahashi, Takashi Kaji, Toshiyuki Yasui, Atsuko Yoshida, Naoto Yonetani, Naoto Suzue, Shinsuke Katoh, Kazuhisa Maeda, Koichi Sairyo, Minoru Irahara and Takeshi Iwasa :
Ultrasonographic changes in quadriceps femoris thickness in women with normal pregnancy and women on bed rest for threatened preterm labor.,
Scientific Reports, Vol.12, No.1, 17506, 2022.

(要約): This study aimed to evaluate the changes in quadriceps femoris muscle thickness during the pregnancy and postpartum periods and to elucidate the effect of bed rest for threatened preterm labor on muscle thickness. In 26 women with normal pregnancy, quadriceps femoris thickness was measured at 11-13, 26, 30, and 35 weeks' gestation, and at 3-5 days and 1 month postpartum using ultrasonography. In 15 pregnant women treated with bed rest for threatened premature labor, quadriceps femoris thickness was measured at 30 and 35 weeks' gestation and postpartum. In women with normal pregnancy, quadriceps femoris thickness increased, peaking at 35 weeks' gestation, followed by a postpartum decrease. In women on bed rest, quadriceps femoris thickness showed no significant change during the pregnancy and postpartum periods, and the muscle was significantly thinner at 35 weeks' gestation than that in women with normal pregnancy. In conclusion, a significant increase in quadriceps femoris muscle thickness during normal pregnancy was found using ultrasonography. Meanwhile, in pregnant women on bed rest treatment, the quadriceps femoris was significantly thinner in the late third trimester than that in normal pregnant women. Prolonged bed rest can affect normal changes in the quadriceps femoris muscle thickness during the pregnancy and postpartum periods.
(キーワード): Infant, Newborn / Humans / Female / Pregnancy / Bed Rest / Quadriceps Muscle / Obstetric Labor, Premature / Gestational Age / Pregnancy Trimester, Third
(徳島大学機関リポジトリ): ● Metadata: 118075
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-022-22467-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36261471; ● Search Scopus @ Elsevier (PMID): 36261471; ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-022-22467-8

(徳島大学機関リポジトリ: 118075, DOI: 10.1038/s41598-022-22467-8, PubMed: 36261471) 湊沙希, 山本由理, 野口拓樹, 内芝舞実, 鎌田周平, 谷口友香, 河北貴子, 苛原稔, 岩佐武 :
当院における精巣内精子採取術・顕微授精(testicular sperm extraction-intracytoplasmic sperm injection: TESE-ICSI)の治療成績と周産期予後の検討,
現代産婦人科, Vol.71, 93-98, 2022年. Shuhei Kamada, Yuri Yamamoto, Hidenori Aoki, Kou Tamura, Asuka Takeda, Saki Minato, Rie Masaki, Rie Yanagihara, Noriko Hayashi, Yuya Yano, Junki Imaizumi, Tomohiro Kagawa, Atsuko Yoshida, Takako Kawakita, Minoru Irahara and Takeshi Iwasa :
A novel PCOS rat model and an evaluation of its reproductive, metabolic, and behavioral phenotypes.,
Reproductive Medicine and Biology, Vol.21, No.1, e12416, 2021.

(要約): Although animal models of PCOS have been used in many studies, none of them can reproduce both the reproductive and metabolic phenotypes of PCOS. In addition, behavioral parameters have not been evaluated in PCOS animal models. We tried to produce an improved rat model of PCOS, and the reproductive, metabolic, and behavioral phenotypes of the model rats were evaluated. Female rats were implanted with silicon tubes containing oil-dissolved dihydrotestosterone (Oil-DHT) as a new PCOS model. Their phenotypes were compared with those of conventional PCOS model rats (DHT), into which tubes containing crystalline DHT were implanted, and non-DHT-treated rats (control). Both the Oil-DHT and DHT rats showed greater body weight gain, food intake, and fat depot weight than the control rats. Furthermore, these groups showed fewer estrous stages and increased numbers of cystic follicles. The DHT rats exhibited lower ovarian and uterine weights than the control rats, whereas no such changes were observed in the Oil-DHT rats. The Oil-DHT and DHT rats showed less locomotor activity in the light phase than the control rats. Our proposed PCOS model reproduced both the reproductive and metabolic phenotypes of PCOS and may have potential for PCOS research.
(徳島大学機関リポジトリ): ● Metadata: 117712
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12416
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34934399; ● Search Scopus @ Elsevier (PMID): 34934399; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12416

(徳島大学機関リポジトリ: 117712, DOI: 10.1002/rmb2.12416, PubMed: 34934399) Natsumi Yamaguchi, Toshiyuki Yasui, Takashi Kaji, Eishi Sogawa, Atsuko Yoshida, Naoto Yonetani and Minoru Irahara :
Usefulness of measurement of proximal phalanx length by using ultrasonography in newborn infants.,
Journal of Obstetrics and Gynaecology, Vol.41, No.5, 739-745, 2020.

(要約): The purpose of this study was to compare digit lengths and proximal phalanx lengths in newborn infants by using ultrasonography (USG) and to examine sex differences between the ratio of the second digit (2D) and fourth digit (4D) of digit length and that of proximal phalanx length and the associations of digit length and proximal phalanx length with birth weight and birth height. Sixty newborn infants (28 males and 32 females) were recruited. Digit lengths were measured by using a transparent ruler and proximal phalanx lengths were measured by using USG. There were significant correlations between digit length and proximal phalanx length in the left and right hands. There was no significant correlation between 2D:4D ratios of digit lengths and 2D:4D ratios of proximal phalanx lengths measured by using USG. In males, birth height was significantly associated with right fourth proximal phalanx length and with left second and fourth proximal phalanx lengths. Birth weight was significantly associated with proximal phalanx length in males. Proximal phalanx length measured by using USG was significantly associated with digit length in newborn infants. Hormonal exposure before birth may be involved in the associations of proximal phalanx length with birth weight and height in males.Impact statement The ratio of the second digit (2D) and fourth digit (4D) has been used postnatally to provide a retrospective indication of the foetal hormonal environment. Digit lengths have been measured by using a direct method, photocopies, digital scans and radiographs, but the results of a study on measurement of digit lengths by using ultrasonography (USG) have not been reported. Proximal phalanx length measured by using USG was significantly associated with digit length in newborn infants. In males, birth height was significantly associated with right fourth proximal phalanx length and with left second and fourth proximal phalanx lengths. Birth weight was significantly associated with proximal phalanx length in males. Measurement of proximal phalanx length by using USG may be useful for a study on gender differences in foetal development and the foetal hormonal environment. Hormonal exposure before birth may be involved in the associations of proximal phalanx length with birth weight and height in males.
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/01443615.2020.1798903
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33054451; ● Search Scopus @ Elsevier (PMID): 33054451; ● Search Scopus @ Elsevier (DOI): 10.1080/01443615.2020.1798903

(DOI: 10.1080/01443615.2020.1798903, PubMed: 33054451) Midori Fukui, Toshihiko Tsutsumi, Aimi Yamamoto-Mikami, Katsuya Morito, Naoko Takahashi, Tamotsu Tanaka, Tekeshi Iwasa, Akira Kuwahara, Minoru Irahara and Akira Tokumura :
Distinct contributions of two choline-producing enzymatic activities to lysophosphatidic acid production in human amniotic fluid from pregnant women in the second trimester and after parturition,
Prostaglandins & Other Lipid Mediators, Vol.150, 106471, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.prostaglandins.2020.106471
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32585250; ● Summary page in Scopus @ Elsevier: 2-s2.0-85087143410

(DOI: 10.1016/j.prostaglandins.2020.106471, PubMed: 32585250, Elsevier: Scopus) 新垣亮輔, 加地剛, 祖川英至, 吉田あつ子, 米谷直人, 苛原稔 :
多尿による羊水過多に対しての頻回の羊水除去を要したBartter症候群の一例,
現代産婦人科, Vol.68, No.2, 245-249, 2020年.

(キーワード): Bartter症候群 / 羊水過多 / 多尿 / 排尿 / Bartter syndrome / polyhydramnios / polyuria / micturition / Bartter ショウコウグン / ヨウスイカタ / タニョウ / ハイニョウ
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572359822903168

(CiNii: 1520572359822903168) Yiliyasi Mayila, Toshiya Matsuzaki, Takeshi Iwasa, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Kiyohito Yano, Rie Yanagihara, Takako Tokui, Saki Minato, Asuka Takeda, Sachiko Endo, Takaaki Maeda and Minoru Irahara :
The reduction in sexual behavior of adult female rats exposed to immune stress in the neonatal period is associated with reduced hypothalamic progesterone receptor expression.,
General and Comparative Endocrinology, Vol.288, 113360, 2020.

(要約): We examined the mechanism by which neonatal immune stress reduces the sexual behavior of female rats in adulthood. Neonatal female rats were randomly divided into 3 groups: control (n = 11), postnatal day 10 lipopolysaccharide (PND10LPS) (n = 23), and PND25LPS (n = 11) groups, which received intraperitoneal injections of LPS (100 μg/kg) or saline on PND10 and 25. Daily inspections of the vaginal opening (VO) were performed from PND27 to PND37. Thereafter, the frequency of estrus was assessed for 15 days. Female rats (at 11-12 weeks of age) were placed in a cage with male rats, and their sexual behavior was monitored for 30 min. The hypothalamic mRNA expression levels of factors related to sexual behavior were examined via real-time PCR. VO occurred later and the frequency of estrus was lower in the PND10LPS group compared to the control group. The number of lordosis behaviors and the total number of mounts performed by male partners were lower in the PND10LPS and PND25LPS groups than in the control group. Acceptability: The lordosis quotient and lordosis rating were lower in the PND10LPS group than in the control group. Proceptive behavior: the number of ear wiggling events was lower in the PND10LPS group than in the other groups, and the number of hops/darts was lower in the PND10LPS group than in the control group. The hypothalamic mRNA expression level of progesterone receptors (PR)A + B was lower in the PND10LPS group than in the control group, and the hypothalamic PRB mRNA expression level was lower in the PND10LPS and PND25LPS groups than in the control group. Neonatal immune stress impeded sexual behavior and hypothalamic PR mRNA expression in female rats. Decreased progesterone activity in the hypothalamus might explain the reduction in sexual behavior seen in these rats.
(キーワード): Age Factors / Animals / Animals, Newborn / Down-Regulation / Drug Administration Schedule / Female / Gene Expression / Hypothalamus / Immune System / Lipopolysaccharides / Male / Pregnancy / Rats / Rats, Sprague-Dawley / Receptors, Progesterone / Sexual Behavior, Animal / Stress, Physiological / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ygcen.2019.113360
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31830472; ● Search Scopus @ Elsevier (PMID): 31830472; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ygcen.2019.113360

(DOI: 10.1016/j.ygcen.2019.113360, PubMed: 31830472) Kiyohito Yano, Toshiya Matsuzaki, Takeshi Iwasa, Yiliyasi Mayila, Rie Yanagihara, Altankhuu Tungalagsuvd, Munkhsaihan Munkhzaya, Takako Tokui, Shuhei Kamada, Aki Hayashi, Rie Masaki, Hidenori Aoki, Kou Tamura and Minoru Irahara :
The influence of psychological stress in early life on sexual maturation and sexual behavior in male and female rats.,
Reproductive Medicine and Biology, Vol.19, No.2, 135-141, 2019.

(要約): We studied the influence of psychological stress during the early neonatal period on sexual maturation and sexual behavior in rats. Neonatal male and female rats were divided into control (C) and maternal separation (MS) groups (n = 20-24 per group). The pups in the MS groups were placed in isolation cages for 240 minutes/d from postnatal days 2-11. Vaginal opening (VO) in females and preputial separation (PS) in males (indicators of sexual maturation) were monitored, as was the estrous cycle in females. Thereafter, sexual behavior was monitored twice at 13 and 15 weeks of age. As for sexual maturation, the onset of PS occurred significantly earlier in the MS group than in the C group, whereas the onset of VO did not differ between the groups. The length of the estrous cycle did not differ between the groups. The frequencies of sexual behaviors did not differ between the groups in either sex. In conclusion, early-life psychological stress induced by MS advanced sexual maturation in male rats, whereas it did not affect sexual maturation in female rats. On the other hand, early-life psychological stress might not affect sexual behavior in adulthood in either sex.
(徳島大学機関リポジトリ): ● Metadata: 114664
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12313
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32273818; ● Search Scopus @ Elsevier (PMID): 32273818; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12313

(徳島大学機関リポジトリ: 114664, DOI: 10.1002/rmb2.12313, PubMed: 32273818) Takeshi Iwasa, Toshiya Matsuzaki, Yiliyasi Mayila, Rie Yanagihara, Yuri Yamamoto, Takako Kawakita, Akira Kuwahara and Minoru Irahara :
Oxytocin treatment reduced food intake and body fat and ameliorated obesity in ovariectomized female rats.,
Neuropeptides, Vol.75, 49-57, 2019.

(要約): Recent studies have shown that oxytocin reduces food intake and body weight gain and promotes lipolysis in some species, including humans. Interestingly, these effects of oxytocin are more marked in obese individuals. Although the menopausal loss of ovarian function induces increased visceral adiposity and some metabolic disorders, no safe medical interventions for these conditions have been established. In this study, we evaluated the effects of oxytocin on appetite, body weight, and fat mass in ovariectomized rats. Six-day oxytocin treatment attenuated cumulative food intake and body weight gain, and reduced visceral and subcutaneous fat weight and adipocyte cell area in ovariectomized rats. Blood examinations indicated that 6-day oxytocin treatment did not alter renal or hepatic functions. Instead, it might prevent ovariectomy-induced liver damage. In addition, acute oxytocin treatment did not affect body temperature or locomotor activity. These results indicate that oxytocin might be useful for treating or preventing menopause-induced metabolic disorders, without causing any adverse effects.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.npep.2019.03.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30885500; ● Search Scopus @ Elsevier (PMID): 30885500; ● Search Scopus @ Elsevier (DOI): 10.1016/j.npep.2019.03.002

(DOI: 10.1016/j.npep.2019.03.002, PubMed: 30885500) Eishi Sogawa, Takashi Kaji, Soichiro Nakayama, Atsuko Yoshida, Naoto Yonetani, Kazuhisa Maeda, Toshiyuki Yasui and Minoru Irahara :
Seasonal variation of serum 25(OH)vitamin D levels in maternal and umbilical cord blood in Japanese women,
The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 128-133, 2019.

(要約): We aimed to demonstrate that the serum 25-hydroxyvitamin D (25(OH)D) level in maternal and umbilical cord blood has a seasonal variation in Japanese women. The study cohort comprised 256 healthy Japanese women with a singleton pregnancy who delivered after 36 gestational weeks between 2012 and 2015. The season at delivery was categorized for 3 months and recorded as "spring", "summer", "autumn" and "winter". Subjects were divided into four groups according to season. A sample of peripheral venous blood at 35-36 gestational weeks and blood from the umbilical vein at delivery were taken. The mean serum 25(OH)D concentra tion (ng/mL) in maternal blood for each season (spring, summer, autumn and winter) was 18.0 (?6.7), 17.1 (?5.1), 21.6 (?8.0) and 16.0 (?5.1), whereas that for umbilical cord blood was 8.8 (?3.6), 8.6 (?2.6), 10.7 (?3.5) and 8.6 (?2.1), respectively. The mean serum 25(OH)D concentration of maternal and umbilical cord blood in autumn was higher than that for the other three seasons. In pregnant Japanese women, the mean serum 25(OH)D concentration in maternal and umbilical cord blood was affected by the season of delivery, with both being highest in autumn. Regardless of the season, the maternal serum concentration of 25(OH)D was low in Japan. J. Med. Invest. 66 : 128-133, February, 2019.
(キーワード): Adult / 女性 (female) / Fetal Blood / Humans / Pregnancy / Seasons / ビタミンD (vitamin D)
(徳島大学機関リポジトリ): ● Metadata: 113353
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.66.128
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064925; ● Summary page in Scopus @ Elsevier: 2-s2.0-85065779659

(徳島大学機関リポジトリ: 113353, DOI: 10.2152/jmi.66.128, PubMed: 31064925, Elsevier: Scopus) Kana Kasai, Takeshi Katou, Yuri Kadota, Otgontsetseg Erdenebayar, Kaoru Keyama, Takako Kawakita, Kanako Yoshida, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Intraperitoneal administration of activin A promotes development of endometriotic lesions in a mouse model of endometriosis.,
The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 123-127, 2019.

(要約): This study aimed to investigate the effect of intraperitoneal administration of activin on the occurrence of endometriosis using a mouse model of endometriosis. A mouse model of endometriosis was prepared by intraperitoneally administering endometrial tissue and blood collected from donor mice to C57BL/6J 7-8- week-old recipient mice. A total of 400 μg of activin A was intraperitoneally administered to model mice in the activin group for 5 days. Intraperitoneal endometriotic lesions were confirmed macroscopically and IL-6 and TNF-α levels in washed ascites were measured by ELISA. Endometriotic lesions were observed in all mice. In the activin group, the maximum diameter of endometriotic lesions was significantly larger than that in control group (4.7?1.3 vs 2.9?0.9 mm, p?0.01). The total area of the lesion was also significantly higher in the activin group than in the control group (21.1?9.9 vs 8.8?5.4 mm,p?0.01). Furthermore, IL-6 and TNF-α levels in ascites were significantly higher in the activin group than in the control group (IL-6 : 85.8?15.3 vs 75.1?19.3 pg/ml, p?0.05 ; TNF-α : 629.8?15.4 vs 605.9?11.4 pg/ml, p?0.05). Activin promotes occurrence of endometriosis. Inflammatory cytokines are also elevated by activin administration,suggesting that they may contribute to progression of endometriosis J. Med. Invest. 66 : 123-127, February, 2019.
(徳島大学機関リポジトリ): ● Metadata: 113270
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.66.123
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064924; ● Search Scopus @ Elsevier (PMID): 31064924; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.66.123

(徳島大学機関リポジトリ: 113270, DOI: 10.2152/jmi.66.123, PubMed: 31064924) Yuu Tanaka, Toshiya Matsuzaki, Naoko Tanaka, Takeshi Iwasa, Akira Kuwahara and Minoru Irahara :
Activin effects on follicular growth in in vitro preantral follicle culture.,
The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 165-171, 2019.

(要約): As the follicular environment transits from being activin dominant to inhibin dominant during folliculogenesis, it is assumed that activin plays an important role in the early stage of follicular growth. We examined the effects of activin on morphological, biochemical and molecular changes in isolated preantral follicles. Preantral follicles were mechanically isolated from 14-day old female C57BL/6 mice. Each follicle was cultured and observed for 14 days usingan in vitro follicle culture system containing FSH, FSH + activin A and FSH + inhibin in the culture medium. We subsequently examined FSH receptor (FSH-R) mRNA expression in isolated follicle cultures with or without activin on days 0 and 2. Activin was observed to significantly stimulate follicle enlargement on days 2, 4, 6 and 8, accelerate morphological changes and increase estradiollevels in culture medium on days 4, 12 and 14. In contrast, inhibin did not alter follicular growth. Additionally, activin stimulated the expression of FSH-R mRNA in isolated granulosa cells. It was demonstrated that activin stimulated the growth of preantral follicles, mainly during the early stage of folliculogenesis, by inducing FSH-R expression, in an isolated follicle culture system. J. Med. Invest. 66 : 165-171, February, 2019.
(徳島大学機関リポジトリ): ● Metadata: 113413
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.66.165
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064932; ● Search Scopus @ Elsevier (PMID): 31064932; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.66.165

(徳島大学機関リポジトリ: 113413, DOI: 10.2152/jmi.66.165, PubMed: 31064932) Atsuko Yoshida, Takashi Kaji, Hirotsugu Yamada, Naoto Yonetani, Eishi Sogawa, Masami Yamao, Kazuhisa Maeda, Masataka Sata and Minoru Irahara :
Measurement of hemodynamics immediately after vaginal delivery in healthy pregnant women by electrical cardiometry.,
The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 75-80, 2019.

(要約): Few reports have focused on hemodynamics around delivery in pregnant women because of the difficulty of continuous and noninvasive measurement. Electrical cardiometry allows noninvasive continuous monitoring of hemodynamics and has recently been used in non-pregnant subjects. We compared the use of electrical cardiometry versus transthoracic echocardiography in healthy pregnant women and evaluated hemodynamics immediately after vaginal delivery. In Study 1, electrical cardiometry and transthoracic echocardiography were used to measure cardiac output in 20 pregnant women with threatened premature delivery. A significant correlation was found between the two methods, with electrical cardiometry showing the higher cardiac output. In Study 2, heart rate, stroke volume, and cardiac output were continuously measured in 15 women during vaginal delivery up to 2 h postpartum. Cardiac output increased markedly because of an increased heart rate and stroke volume at the time of newborn delivery. The heart rate then immediately returned to baseline, while cardiac output remained elevated for at least 2 h after delivery because of a sustained high stroke volume. Electrical cardiometry was as readily available as transthoracic echocardiography for evaluating hemodynamics and allowed for continuous measurement during labor. High intrapartum cardiac output was sustained for at least 2 h after vaginal delivery. J. Med. Invest. 66 : 75-80, February, 2019.
(徳島大学機関リポジトリ): ● Metadata: 113325
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.66.75
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064959; ● Search Scopus @ Elsevier (PMID): 31064959; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.66.75

(徳島大学機関リポジトリ: 113325, DOI: 10.2152/jmi.66.75, PubMed: 31064959) Ayuka Mineda, Masato Nishimura, Tomohiro Kagawa, Eri Takiguchi, Takako Kawakita, Akiko Abe and Minoru Irahara :
Resveratrol suppresses proliferation and induces apoptosis of uterine sarcoma cells by inhibiting the Wnt signaling pathway.,
Experimental and Therapeutic Medicine, Vol.17, No.3, 2242-2246, 2019.

(要約): Resveratrol, a natural product and peroxisome proliferator-activated receptor (PPAR) agonist, has been reported to exert anti-cancer effects in several tumor models. A previous study by our group reported that prostaglandin J2, a PPARγ ligand, inhibited cell proliferation in a uterine sarcoma cell line. The aim of the present study was to investigate the role of the Wnt signaling pathway in resveratrol-induced apoptosis and inhibition of cell proliferation in the MES-SA human uterine sarcoma cell line. A WST-1 assay demonstrated that resveratrol inhibited cell proliferation in the MES-SA cell line, and flow cytometry revealed that the number of apoptotic cells increased in a resveratrol dose-dependent manner. The mechanisms underlying these effects of resveratrol were speculated to involve the expression of β-catenin and its target gene, c-myc, which were examined using western blot analysis. The results revealed a dose-dependent downregulation of this β-catenin and c-myc. This effect was blunted by a pharmacological inhibitor of glycogen synthase kinase 3β. Therefore, it is likely that resveratrol inhibited the cell proliferation and increased the number of apoptotic cells, at least partially, via the Wnt signaling pathway. The present results suggest that resveratrol is a potential candidate for the treatment of uterine sarcoma.
(徳島大学機関リポジトリ): ● Metadata: 113338
(出版サイトへのリンク): ● Publication site (DOI): 10.3892/etm.2019.7209
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30867708; ● Search Scopus @ Elsevier (PMID): 30867708; ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2019.7209

(徳島大学機関リポジトリ: 113338, DOI: 10.3892/etm.2019.7209, PubMed: 30867708) Sumika Matsui, Toshiyuki Yasui, Hirokazu Uemura and Minoru Irahara :
Variation in follicle-stimulating hormone level and different associations of follicle-stimulating hormone level with body mass index over the menopausal transition in Japanese wome,
The Journal of Japan Society for Menopause and Women's Health, Vol.27, No.2, 311-317, 2019.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572357894035456

(CiNii: 1520572357894035456) Kaoru Keyama, Takeshi Katou, Yuri Kadota, Otgontsetseg Erdenebayar, Kana Kasai, Takako Kawakita, Anna Tani, Sumika Matsui, Takeshi Iwasa, Kanako Yoshida, Masahiko Maegawa, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Lipopolysaccharide promotes early endometrial-peritoneal interactions in a mouse model of endometriosis.,
The Journal of Medical Investigation : JMI, Vol.66, No.1.2, 70-74, 2019.

(要約): The aims of this study were to clarify the effects of lipopolysaccharide (LPS) on the early development of endometriosis and on the production of cytokines and chemokines in the murine peritoneal cavity. Endometriotic lesions were induced in C57BL/6J adult female mice by intraperitoneal injection of endometrial fragments plus blood or endometrial fragments plus blood with LPS. On day 7, endometriotic lesions were assessed by gross and microscopic evaluations. Time-dependent changes in the secretion of TNF-α,IL-6,and CXCL2/MIP-2 in peritoneal lavage fluid after the intraperitoneal injection of LPS (50 µg/body) were measured by their respective enzyme-linked immunosorbent assays. The areas of endometriotic lesions in the LPS group (10.8 8.6 mm) were significantly larger than those in the control group (3.1 3.7 mm).The levels of TNF-α and IL-6 peaked within 2 hours and the level of MIP-2 reached a maximum on day 1 after the injection of LPS. LPS promotes development of the early stages of murine endometriotic lesions. J. Med. Invest. 66 : 70-74, February, 2019.
(徳島大学機関リポジトリ): ● Metadata: 113324
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.66.70
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31064958; ● Search Scopus @ Elsevier (PMID): 31064958; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.66.70

(徳島大学機関リポジトリ: 113324, DOI: 10.2152/jmi.66.70, PubMed: 31064958) 岩佐武, 谷口友香, 中山聡一朗, 米谷直人, 吉田あつ子, 阿部彰子, 山本由理, 加地剛, 桑原章, 松崎利也, 苛原稔 :
徳島大学病院産科婦人科における不育症患者に対する染色体検査の実施状況,
現代産婦人科, Vol.67, No.1, 21-24, 2018年.

(キーワード): 不育症 / 習慣流産 / 染色体検査 / recurrent miscarriage / chromosome analysis
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572359817582976

(CiNii: 1520572359817582976) Osamu Ishihara, Seung Chik Jwa, Akira Kuwahara, Tomonori Ishikawa, Koji Kugu, Rintaro Sawa, Kouji Banno, Minoru Irahara and Hidekazu Saito :
Assisted reproductive technology in Japan: A summary report for 2016 by the Ethics Committee of the Japan Society of Obstetrics and Gynecology.,
Reproductive Medicine and Biology, Vol.18, No.1, 7-16, 2018.

(要約): The Japan Society of Obstetrics and Gynecology started an online cycle-based assisted reproductive technology (ART) registry system in 2007. This report presents the characteristics and treatment outcomes of ART registered for the cycles practiced during 2016. Cycle-specific information for all ART cycles implemented in participating ART facilities were collected. A descriptive analysis was conducted for the registry database of 2016. In total, 447 790 treatment cycles and 54 110 neonates (one in 18.1 neonates born in Japan) were reported in 2016. The mean patients' age was 38.1 years (SD = 4.5). Among the egg retrieval cycles, 104 575 of 251 399 (41.6%) were freeze-all cycles without fresh embryo transfers (ET), while fresh ET was performed in 64 497 cycles (58.4%). A total of 187 132 frozen-thawed ET cycles were reported, resulting in 62 432 pregnancies and 44 484 neonates born. Single ET was selected for 81.0% of fresh transfers and 82.7% of frozen cycles, resulting in singleton pregnancy/live birth rates of 97.0%/96.4% and 96.7%/96.4%, respectively. The total ART cycles and subsequent live births continued to increase in 2016. Single ET was performed more than 80%, and ET has shifted from using fresh embryos to frozen ones.
(徳島大学機関リポジトリ): ● Metadata: 114594
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12258
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30655717; ● Search Scopus @ Elsevier (PMID): 30655717; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12258

(徳島大学機関リポジトリ: 114594, DOI: 10.1002/rmb2.12258, PubMed: 30655717) Toshiya Matsuzaki, Munkhsaikhan Munkhzaya, Takeshi Iwasa, Altankhuu Tungalagsuvd, Kiyohito Yano, Yiliyasi Mayila, Rie Yanagihara, Takako Tokui, Takeshi Katou, Akira Kuwahara, Sumika Matsui and Minoru Irahara :
Prenatal undernutrition suppresses sexual behavior in female rats.,
General and Comparative Endocrinology, Vol.269, 46-52, 2018.

(要約): Infectious, psychological and metabolic stresses in the prenatal and early neonatal period induce long-lasting effects in physiological function and increase the risk of metabolic disorders later in life. We examined the sexual behavior of female rats that were subjected to undernutrition in the prenatal period. Eight pregnant rats were divided into two groups: a maternal normal nutrition group (mNN; n = 4) and a maternal undernutrition group (mUN; n = 4), which received 50% of the daily food intake amount of the mNN group from gestation day 13 to delivery. Nine and seven female offspring were randomly selected from the mNN and mUN groups, respectively. Vaginal opening (VO), estrous cycle length, sexual behavior and mRNA expression levels of the factors that regulate sexual behavior were observed. In the mUN group, VO day was later, the estrous cycle was longer, and the lordosis quotient and lordosis rating were lower than in the mNN group; such differences were not seen in other sexual performances, such as ear wiggles, darts, kick bouts and box. The hypothalamic mRNA expression level of progesterone receptor (PR) A + B and oxytocin (OT) were significantly lower in the mUN group than in the mNN group. These findings indicated that prenatal undernutrition disrupted puberty onset, the estrous cycle, sexual behavior and hypothalamic mRNA expression of PR and OT in female rat pups.
(キーワード): Amygdala / Animals / Body Weight / Eating / Estrous Cycle / Female / Hypothalamus / Malnutrition / Pregnancy / Prenatal Exposure Delayed Effects / RNA, Messenger / Rats, Sprague-Dawley / Sexual Behavior / Sexual Maturation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ygcen.2018.08.013
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30099033; ● Search Scopus @ Elsevier (PMID): 30099033; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ygcen.2018.08.013

(DOI: 10.1016/j.ygcen.2018.08.013, PubMed: 30099033) Kanako Yoshida, Masato Nishimura, Akiko Abe, Takeshi Katou, Hiroyuki Furumoto and Minoru Irahara :
Can systematic lymphadenectomy be omitted for low-risk endometrial cancer?,
The Journal of Medical Investigation : JMI, Vol.65, No.3,4, 221-224, 2018.

(要約): The objective of this study was to identify pathological indicators that could be used to identify a subgroup of patients with apparent stage I endometrial cancer who do require retroperitoneal lymphadenectomy. 188 T1 endometrial cancer patients underwent primary surgery at Tokushima University Hospital. We retrospectively evaluated their clinical records and histopathological factors. Systematic lymphadenectomy was performed for 149 patients, and 39 patients (grade 1 with < 5 mm of myometrial invasion) were treated without lymphadenectomy. Lymph node metastases were found in 19 (12.8%) of the lymphadenectomy cases. Twenty-four patients with a T1a endometrium-limited lesion did not exhibit lymph node metastasis. Three (3.1%) of the 95 patients with a T1a lesion exhibited lymph node metastasis, and these 3 cases exhibited approximately 50% myometrial invasion. The 39 low-risk patients who did not undergo systematic lymphadenectomy remain alive without recurrence. Systematic lymphadenectomy could be omitted for patients with a grade 1 tumor and minor myometrial invasion of less than 5mm. J. Med. Invest. 65:221-224, August, 2018.
(キーワード): Adult / Aged / Aged, 80 and over / Endometrial Neoplasms / Female / Humans / Lymph Node Excision / Lymphatic Metastasis / Middle Aged / Myometrium / Neoplasm Staging / Retrospective Studies / Risk Factors / Young Adult
(徳島大学機関リポジトリ): ● Metadata: 112242
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.65.221
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30282864; ● Search Scopus @ Elsevier (PMID): 30282864; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.65.221

(徳島大学機関リポジトリ: 112242, DOI: 10.2152/jmi.65.221, PubMed: 30282864) Tomoko Yoshida, Toshiya Matsuzaki, Mami Miyado, Kazuki Saito, Takeshi Iwasa, Yoichi Matsubara, Tsutomu Ogata, Minoru Irahara and Maki Fukami :
11-oxygenated C19 steroids as circulating androgens in women with polycystic ovary syndrome.,
Endocrine Journal, Vol.65, No.10, 979-990, 2018.

(要約): 11-oxygenated C19 steroids (11oxC19s) are newly specified human androgens. Although median serum levels of 11oxC19 were reported to be higher in patients with polycystic ovary syndrome (PCOS) than in unaffected women, inter-individual variations in androgen levels among PCOS patients have poorly been investigated. Here, we quantified four 11oxC19s, i.e., 11-ketotestosterone (11KT), 11β-hydroxytestosterone (11OHT), 11β-hydroxyandrostenedione (11OHΔ4A), and 11-ketoandrostenedione (11KΔ4A), in blood samples of 28 PCOS patients and 31 eumenorrheic women using liquid chromatography-tandem mass spectrometry. We referred to our previous data of classic androgens in these individuals. We found that 11OHT levels were higher in the PCOS group than in the eumenorrheic group. Moreover, although the median values of 11KT, 11KΔ4A, and 11OHΔ4A were comparable between the two groups, these steroids were markedly increased in some patients. Of the 28 patients, 8 had high levels of both 11oxC19s and classic androgens, whereas 4 had an increase only in 11oxC19 levels, and 12 had an increase only in classic androgen levels. Intragroup variations in androgen levels were relatively large in the PCOS group. Levels of 11OHT and 11KT were significantly higher in overweight/obese patients than in normal weight patients and correlated with body mass indexes. These results highlight the clinical significance of 11oxC19s as circulating androgens in PCOS patients and indicate that the accumulation of 11oxC19s and/or classic androgens is an essential feature of PCOS. The profiles of circulating androgens appear to vary among patients. In particular, overweight/obesity likely enhances the 11oxC19s accumulation in PCOS, although this notion awaits further validation.
(キーワード): Adult / Androgens / Body Mass Index / Chromatography, Liquid / Female / Humans / Obesity / Polycystic Ovary Syndrome / Tandem Mass Spectrometry / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ18-0212
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30012903; ● Search Scopus @ Elsevier (PMID): 30012903; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ18-0212

(DOI: 10.1507/endocrj.EJ18-0212, PubMed: 30012903) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Rie Yanagihara, Yiliyasi Mayila and Minoru Irahara :
The effects of chronic testosterone administration on hypothalamic gonadotropin-releasing hormone regulatory factors (Kiss1, NKB, pDyn and RFRP) and their receptors in female rats.,
Gynecological Endocrinology, Vol.34, No.5, 437-441, 2018.

(要約): The effects of androgens on gonadotropin-releasing hormone (GnRH) secretion in females have not been fully established. To clarify the direct effects of androgens on hypothalamic reproductive factors, we evaluated the effects of chronic testosterone administration on hypothalamic GnRH regulatory factors in ovariectomized (OVX) female rats. Both testosterone and estradiol reduced the serum luteinizing hormone levels of OVX female rats, indicating that, as has been found for estrogen, testosterone suppresses GnRH secretion via negative feedback. Similarly, the administration of testosterone or estradiol suppressed the hypothalamic mRNA levels of kisspeptin and neurokinin B, both of which are positive regulators of GnRH, whereas it did not affect the hypothalamic mRNA levels of the kisspeptin receptor or neurokinin-3 receptor. On the contrary, the administration of testosterone, but not estradiol, suppressed the hypothalamic mRNA expression of prodynorphin, which is a negative regulator of GnRH. The administration of testosterone did not alter the rats' serum estradiol levels, indicating that testosterone's effects on hypothalamic factors might be induced by its androgenic activity. These findings suggest that as well as estrogen, androgens have negative feedback effects on GnRH in females and that the underlying mechanisms responsible for these effects are similar, but do not completely correspond, to the mechanisms underlying the effects of estrogen on GnRH.
(徳島大学機関リポジトリ): ● Metadata: 112451
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09513590.2017.1409709
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29187003; ● Search Scopus @ Elsevier (PMID): 29187003; ● Search Scopus @ Elsevier (DOI): 10.1080/09513590.2017.1409709

(徳島大学機関リポジトリ: 112451, DOI: 10.1080/09513590.2017.1409709, PubMed: 29187003) Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Takeshi Iwasa, Kiyohito Yano, Yiliyasi Mayila, Takako Tokui, Rie Yanagihara, Sumika Matsui, Takeshi Katou, Akira Kuwahara and Minoru Irahara :
Neurokinin B receptor agonist and Dynorphin receptor antagonist stimulated luteinizing hormone secretion in fasted male rodents.,
Endocrine Journal, Vol.65, No.4, 485-492, 2018.

(要約): Kisspeptin/neurokinin B (NKB)/dynorphin (Dyn) (KNDy) neuron in hypothalamic arcuate nucleus plays a key role in GnRH/LH pulsatile secretion. We aimed to determine whether stimulation of NKB/neurokinin 3 receptor (NK3R) signaling and inhibition of Dyn/kappa-opioid receptor (KOR) signaling recover LH secretion that is suppressed by acute fasting in male rats. Furthermore, we determined dose dependent effect of NKB/NK3R signaling on serum LH level under acute fasting condition in male mice. Mature male rats were injected saline (0.1 mL) and senktide (20 μg/kg), a NK3R agonist, or nor-BNI (800 μg/kg), a KOR antagonist intraperitoneally (ip) after 72 h fasting. And mature male mice were injected multiple doses of senktide, ip after 48 h fasting. Blood and brain sample were collected 90 min after injections for LH measurement and hypothalamic mRNA expressions. All three studies showed significantly lower LH concentration in fasted groups than non-fasted groups. Senktide did not recover LH suppressed by acute fasting in male rats, whereas nor-BNI injected male rats showed significantly higher LH than 72 h fasted male rats (p < 0.05). Mice study showed significantly higher LH concentration in higher doses senktide groups than 48 h fasted group and one of lower doses senktide group. These results suggest that stimulation of NKB/NK3R signaling and attenuation of Dyn/KOR signaling could recover suppressed LH secretion under acute fasting condition in male rodents.
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ17-0136
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29479004; ● Search Scopus @ Elsevier (PMID): 29479004; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ17-0136

(DOI: 10.1507/endocrj.EJ17-0136, PubMed: 29479004) Masaharu Kamada, Hiroaki Inui, Tomohiro Kagawa, Ayuka Mineda, Takao Tamura, Tomohito Fujioka, Takahiro Motoki, Hiroki Hirai, Eiichi Ishii and Minoru Irahara :
What information can change the attitude of teachers toward the human papillomavirus vaccine?,
The Journal of Obstetrics and Gynaecology Research, Vol.44, No.4, 778-787, 2018.

(要約): We conducted a self-administered survey on the perception of teachers toward human papillomavirus (HPV) vaccine to determine the ways to increase their willingness to encourage its use. Answers were obtained both prior to and after having the teachers read five brief information articles: (i) cervical cancer knowledge, (ii) vaccine knowledge, (iii) result of a survey in Nagoya, (iv) news report of the World Health Organization statement and (v) articles written by Dr Muranaka, a journalist. Most of the respondents (180/247) did not know about the natural history of cervical cancer. Only 36% knew that HPV is the cause of cervical cancer, although 63% knew that HPV vaccine would prevent cervical cancer. Few respondents had knowledge regarding adverse events following immunization and the survey results from Nagoya. Among those who were initially negative for the HPV vaccine, only 43% revealed that they fully understood its safety and only 29% reversed their opinion to recommend vaccination to their daughters and/or students, even after reading our informational material. The most useful information for changing their attitudes was to increase their understanding of vaccines and informing them about Nagoya city survey results. They mostly wanted a proof of the preventive effects of the vaccine on cervical cancer in Japan. Gynecologists and pediatricians must proactively communicate accurate scientific information to the government and the media to spread awareness among people in Japan. Also, we must try to demonstrate the capabilities of this vaccine to prevent cervical cancer and/or its precancerous lesions.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jog.13584
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29442400; ● Search Scopus @ Elsevier (PMID): 29442400; ● Search Scopus @ Elsevier (DOI): 10.1111/jog.13584

(DOI: 10.1111/jog.13584, PubMed: 29442400) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Yiliyasi Mayila and Minoru Irahara :
Effects of dihydrotestosterone administration on the expression of reproductive and body weight regulatory factors in ovariectomized and estradiol-treated female rats.,
Gynecological Endocrinology, Vol.34, No.1, 73-77, 2018.

(要約): To clarify the direct effects of androgens, the changes in the hypothalamic levels of reproductive and appetite regulatory factors induced by chronic dihydrotestosterone (DHT) administration were evaluated in female rats. DHT treatment increased the BW and food intake of the ovariectomized rats, but not the estradiol (E2)-treated rats. DHT administration suppressed the expression of a hypothalamic anorexigenic factor. Although the kisspeptin (Kiss1) mRNA levels of the anterior hypothalamic block (the anteroventral periventricular nucleus, AVPV) were increased in the E2-treated rats, DHT administration did not affect the Kiss1 mRNA levels of the AVPV in the ovariectomized or E2-treated rats. Conversely, DHT administration reduced the Kiss1 mRNA levels of the posterior hypothalamic block (the arcuate nucleus, ARC) in the ovariectomized rats. Although the Kiss1 mRNA levels of the posterior hypothalamic block (ARC) were decreased in the E2-treated rats, DHT administration did not affect the Kiss1 mRNA levels of the ARC in these rats. Serum luteinizing hormone levels of these groups exhibited similar patterns to the Kiss1 mRNA levels of the ARC. These results showed that DHT affects the production of hypothalamic reproductive and appetite regulatory factors, and that these effects of DHT differ according to the estrogen milieu.
(徳島大学機関リポジトリ): ● Metadata: 112455
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09513590.2017.1337096
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28604137; ● Search Scopus @ Elsevier (PMID): 28604137; ● Search Scopus @ Elsevier (DOI): 10.1080/09513590.2017.1337096

(徳島大学機関リポジトリ: 112455, DOI: 10.1080/09513590.2017.1337096, PubMed: 28604137) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Yiliyasi Mayila and Minoru Irahara :
The roles of kisspeptin and gonadotropin inhibitory hormone in stress-induced reproductive disorders.,
Endocrine Journal, Vol.65, No.2, 133-140, 2018.

(要約): Several kinds of stress suppress the hypothalamic-pituitary-gonadal (HPG) axis and reproductive behavior in humans and animals. These changes can eventually cause diseases and disorders, such as amenorrhea and infertility. In previous studies, it has been shown that stress-related factors, e.g., corticotropin-releasing hormone, cortisol, and pro-inflammatory cytokines, promote the stress-induced suppression of the HPG axis. However, these mechanisms are not sufficient to explain how stress suppresses HPG axis activity, and it has been suggested that some other factors might also be involved. In the early 21st century, novel neuroendocrine peptides, kisspeptin and gonadotropin inhibitory hormone (GnIH)/RFamide-related peptide 3 (RFRP-3), which directly regulate GnRH/gonadotropin synthesis and secretion, were newly discovered. Growing evidence indicates that kisspeptin and GnIH/RFRP-3 play pivotal roles in the stress-induced disruption of the HPG axis and reproductive behavior in addition to their physiological functions. This review summarizes what is currently known about the roles of kisspeptin and GnIH/RFRP-3 in stress-induced reproductive disorders.
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ18-0026
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29375085; ● Search Scopus @ Elsevier (PMID): 29375085; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ18-0026

(DOI: 10.1507/endocrj.EJ18-0026, PubMed: 29375085) Akiko Abe, Masato Nishimura and Minoru Irahara :
See and treat LEEP biopsy for cervical intraepithelial neoplasia grade 2/3,
European JOURNAL of Gynaecological Oncology, Vol.39, No.6, 958-962, 2018.

(徳島大学機関リポジトリ): ● Metadata: 113734
(出版サイトへのリンク): ● Publication site (DOI): 10.12892/ejgo4391.2018
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85058843418

(徳島大学機関リポジトリ: 113734, DOI: 10.12892/ejgo4391.2018, Elsevier: Scopus) Aya Shirakawa, Takashi Kaji, Yasunobu Hayabuchi, Souichiro Nakayama, Kazuhisa Maeda and Minoru Irahara :
Prenatal three-dimensional color Doppler imaging showing crossover of the inflow streams of two ventricles in a case of criss-cross heart.,
Journal of Echocardiography, Vol.15, No.4, 191-193, 2017.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12574-017-0339-3
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28484959; ● Search Scopus @ Elsevier (PMID): 28484959; ● Search Scopus @ Elsevier (DOI): 10.1007/s12574-017-0339-3

(DOI: 10.1007/s12574-017-0339-3, PubMed: 28484959) Hidekazu Saito, Seung Chik Jwa, Akira Kuwahara, Kazuki Saito, Tomonori Ishikawa, Osamu Ishihara, Koji Kugu, Rintaro Sawa, Kouji Banno and Minoru Irahara :
Assisted reproductive technology in Japan: a summary report for 2015 by The Ethics Committee of The Japan Society of Obstetrics and Gynecology.,
Reproductive Medicine and Biology, Vol.17, No.1, 20-28, 2017.

(要約): The Japan Society of Obstetrics and Gynecology (JSOG) implemented an assisted reproductive technology (ART) registry system in 1986. Here are reported the characteristics and treatment outcomes of ART cycles that were registered in 2015. JSOG has requested all participating ART facilities to register cycle-specific information for all ART cycles since 2007. A descriptive analysis was performed by using the registry database for 2015. In total, 424 151 cycles and 51 001 neonates (1 in 19.7 neonates born in Japan) were registered in 2015. The patients' mean age was 38.2 years (standard deviation = 4.5). Among the fresh cycles, 94 158 of 244 718 (38.5%) egg retrieval cycles were cycles with freeze-all embryos or oocytes, while fresh embryo transfer (ET) was performed in 70 254 cycles, signaling a decrease from 2014. There were 169 898 frozen-thawed ET cycles, resulting in 56 355 pregnancies and 40 599 neonates. Single ET was performed at a rate of 79.7% for fresh and 81.8% for frozen cycles and the singleton pregnancy/live birth rates were 96.9%/96.5% and 96.8%/96.4% for the respective cycles. The total ART cycles and live births resulting from ART has been increasing in Japan. Single ET was performed at a rate of almost 80% and ET cycles have shifted from fresh to frozen cycles.
(徳島大学機関リポジトリ): ● Metadata: 112970
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12074
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29371817; ● Search Scopus @ Elsevier (PMID): 29371817; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12074

(徳島大学機関リポジトリ: 112970, DOI: 10.1002/rmb2.12074, PubMed: 29371817) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano and Minoru Irahara :
The effects of ovariectomy and lifelong high-fat diet consumption on body weight, appetite, and lifespan in female rats.,
Hormones and Behavior, Vol.97, 25-30, 2017.

(要約): In females, ovarian hormones play pivotal roles in metabolic, appetite, and body weight regulation. In addition, it has been reported that ovarian hormones also affect longevity in some species. Recently, it was found that the consumption of a high-fat diet aggravates ovariectomy-associated metabolic dysregulation in female rodents. The aim of this study was to investigate the hypothesis that long-term high-fat diet consumption and ovariectomy interact to worsen body weight regulation and longevity in female rats. At 21days of age, female rats were weaned and randomly divided into two groups, one of which was given the high-fat diet, and the other was supplied with standard chow. At 23weeks of age, each group was further divided into ovariectomized and sham-operated groups, and then their body weight changes, food intake, and longevity were measured until 34months of age. The sham - high-fat diet rats exhibited greater body weight changes and higher feed efficiency than the sham - standard chow rats. On the other hand, the ovariectomized - high-fat diet and ovariectomized - standard chow rats displayed similar body weight changes and feed efficiency. The sham - high-fat diet and ovariectomized - standard chow rats demonstrated similar body weight changes and feed efficiency, indicating that the impact of ovariectomy on the regulation of body weight and energy metabolism might be similar to that of high-fat diet. Contrary to our expectations, ovariectomy and high-fat diet consumption both had small favorable effects on longevity. As the high-fat diet used in the present study not only had a high fat content, but also had a high caloric content and a low carbohydrate content compared with the standard chow, it is possible that the effects of the high-fat diet on body weight and longevity were partially induced by its caloric/carbohydrate contents. These findings indicate that the alterations in body weight and energy metabolism induced by ovariectomy and high-fat diet might not directly affect the lifespan of female rats.
(徳島大学機関リポジトリ): ● Metadata: 112452
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.yhbeh.2017.10.005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29054797; ● Search Scopus @ Elsevier (PMID): 29054797; ● Search Scopus @ Elsevier (DOI): 10.1016/j.yhbeh.2017.10.005

(徳島大学機関リポジトリ: 112452, DOI: 10.1016/j.yhbeh.2017.10.005, PubMed: 29054797) Yoshimasa Asada, Yoshiharu Morimoto, Yoshiharu Nakaoka, Takahiro Yamasaki, Yutaka Suehiro, Hikaru Sugimoto, Masayuki Yoshida and Minoru Irahara :
Age-specific serum anti-Müllerian hormone concentration in Japanese women and its usefulness as a predictor of the ovarian response.,
Reproductive Medicine and Biology, Vol.16, No.4, 364-373, 2017.

(要約): To compare the ovarian response predictive ability of anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH), and estradiol (E2) and to determine the age-specific distribution of serum AMH concentrations of Japanese women. This was a multicenter (four-site), observational, analytic, cross-sectional Japanese study consisting of two parts: Study 1 (the prediction of the ovarian response of 236 participants who were undergoing controlled ovarian stimulation [COS]) and Study 2 (the distribution of the AMH concentration with an assay of 417 healthy women who were aged 20-49 years and who had normal menstrual cycles). The AMH had a significantly higher predictive value for the normal and high responders than FSH and E2 as a stronger correlation between the ovarian response and AMH was observed than for FSH and E2. The serum AMH concentration decreased proportionally with age. The AMH concentration correlated well with the oocyte count in the patients who were undergoing COS for in vitro fertilization and was shown to predict the risk of ovarian hyperstimulation syndrome among these patients.
(徳島大学機関リポジトリ): ● Metadata: 112968
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12055
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259490; ● Search Scopus @ Elsevier (PMID): 29259490; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12055

(徳島大学機関リポジトリ: 112968, DOI: 10.1002/rmb2.12055, PubMed: 29259490) Toshiya Matsuzaki, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Yiliyasi Mayila, Takeshi Iwasa, Kiyohito Yano, Rie Yanagihara, Takako Tokui, Takeshi Katou, Akira Kuwahara, Sumika Matsui and Minoru Irahara :
Prenatal undernutrition disrupted the sexual maturation, but not the sexual behavior, in male rats.,
Reproductive Medicine and Biology, Vol.16, No.4, 325-329, 2017.

(要約): Exposure to various stressors, including psychological, metabolic, and immune, in the perinatal period induces long-lasting effects in physiological function and increase the risk of metabolic disorders in later life. In the present study, sexual maturation and sexual behavior were assessed in prenatally undernourished mature male rats. All the pregnant rats were divided into the maternal normal nutrition (mNN) group and the maternal undernutrition (mUN) group. The mUN mothers received 50% of the amount of the daily food intake of the mNN mothers. Preputial separation and sexual behavior were observed in randomly selected pups of the mNN and mUN groups. The body weight of the mothers was significantly lighter in the mUN group than in the mNN group. Similarly, the pups in the mUN group showed a significantly lower body weight than those in the mNN group from postnatal day (PND) 1 to PND 15. The preputial separation day was significantly delayed in the mUN group, compared to the mNN group. Sexual behavior did not show any significant difference between the two groups. These findings indicated that prenatal undernutrition delayed sexual maturation, but did not suppress sexual behavior, in mature male rats.
(徳島大学機関リポジトリ): ● Metadata: 112967
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12045
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259485; ● Search Scopus @ Elsevier (PMID): 29259485; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12045

(徳島大学機関リポジトリ: 112967, DOI: 10.1002/rmb2.12045, PubMed: 29259485) Yuka Taniguchi, Akira Kuwahara, Ayaka Tachibana, Yuya Yano, Kiyohito Yano, Yuri Yamamoto, Mikio Yamasaki, Takeshi Iwasa, Kenji Hinokio, Toshiya Matsuzaki and Minoru Irahara :
Intra-follicular kisspeptin levels are related to oocyte maturation and gonadal hormones in patients who are undergoing assisted reproductive technology.,
Reproductive Medicine and Biology, Vol.16, No.4, 380-385, 2017.

(要約): To assess the kisspeptin concentrations in follicular fluid and their relationship with clinical outcomes during assisted reproductive technology. Thirty-nine patients who were aged 24-40 years and underwent oocyte retrieval for in vitro fertilization/intracytoplasmic sperm injection participated in this study In 65 follicular fluid samples that had been obtained from 30 patients and their blood samples, the kisspeptin levels were measured in order to investigate the correlations with their gonadal hormone levels. Venous blood samples were collected from 14 patients to investigate their plasma kisspeptin levels across different phases of assisted reproductive technology. The follicular fluid kisspeptin level was significantly higher than that of the plasma level and was positively associated with the follicular fluid estradiol concentration and with the serum estradiol and number of mature oocytes. In the plasma, the maximum concentration of kisspeptin was observed on the day of ovum pick-up and on the day of embryo transfer during ovarian stimulation for assisted reproductive technology. Kisspeptin was present in the follicular fluid and the plasma kisspeptin concentration was affected by ovarian stimulation. Kisspeptin appears to affect oocyte maturation and ovulation.
(徳島大学機関リポジトリ): ● Metadata: 112881
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12056
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259492; ● Search Scopus @ Elsevier (PMID): 29259492; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12056

(徳島大学機関リポジトリ: 112881, DOI: 10.1002/rmb2.12056, PubMed: 29259492) Sumika Matsui, Toshiyuki Yasui, Kana Kasai, Kanako Yoshida, Takeshi Katou, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Circulating dehydroepiandrosterone-sulphate decreases even with a slight change in oestradiol.,
Journal of Obstetrics and Gynaecology, Vol.38, No.2, 231-235, 2017.

(要約): The aim of this study was to determine the effect of hormone replacement therapy (HRT) on changes in circulating dehydroepiandrosterone-sulphate (DHEA-S) with focus on the relationship between oestrogen level and change in DHEA-S. Forty-two women were enrolled in this longitudinal study. Nineteen women received oral oestradiol and twenty-three women received transdermal oestradiol continuously. Twenty women received progesterone continuously except for women who had undergone hysterectomy. Circulating oestradiol, follicle-stimulating hormone, luteinising hormone and DHEA-S levels before and at 3 months after commencement of HRT were measured. Circulating DHEA-S level was significantly decreased at 3 months (p < .001). Oestradiol level at 3 months ranged from 6.5 pg/ml to 159 pg/ml. There was no significant correlation of ΔDHEA-S (DHEAS level at 3 months-DHEA-S level at baseline) with Δoestradiol (r = 0.114, p = .471). Circulating DHEA-S level was significantly decreased at 3 months in all the four quartiles and divided according to Δoestradiol, and ΔDHEA-S did not show significant differences. In conclusion, circulating DHEA-S decreases even with a slight increase in oestradiol level. Impact statement What is already known on this subject: A transient increase in DHEA-S in women during the menopausal transition may be involved in the occurrence of menopausal symptoms and/or unfavourable metabolic changes. Hormone replacement therapy decreases circulating DHEA-S level. However, dose dependency of the change in DHEA-S on oestrogen has not been reported. What the results of this study add: Circulating DHEA-S decreases even with a slight increase in oestradiol level. What the implications are of these findings for clinical practice and/or further research: Adrenal function may respond to a small change in oestrogen.
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/01443615.2017.1344829
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28903616; ● Search Scopus @ Elsevier (PMID): 28903616; ● Search Scopus @ Elsevier (DOI): 10.1080/01443615.2017.1344829

(DOI: 10.1080/01443615.2017.1344829, PubMed: 28903616) Takeshi Iwasa, Toshiya Matsuzaki, Mayila Yiliyasi, Kiyohito Yano and Minoru Irahara :
The effects of chronic testosterone administration on body weight, food intake, and fat weight were age-dependent.,
Steroids, Vol.127, 18-23, 2017.

(要約): Previously, we showed that chronic testosterone administration increased body weight (BW) and food intake (FI), but did not alter fat weight, in young female rats. To examine our hypothesis that the effects of androgens on BW, FI and body composition might be age-dependent, the effects of chronic testosterone administration were evaluated in rats of different ages; i.e., young and middle-aged rats. Although chronic testosterone administration increased BW gain, FI, and feed efficiency in both young and middle-aged rats, it increased visceral fat weight in middle-aged rats, but not in young rats. Therefore, it is possible that testosterone promotes the conversion of energy to adipose tissue and exacerbates fat accumulation in older individuals. In addition, although the administration of testosterone increased the serum leptin level, it did not alter hypothalamic neuropeptide Y mRNA expression in middle-aged rats. On the contrary, the administration of testosterone did not affect the serum leptin levels of young rats. Thus, testosterone might induce hypothalamic leptin resistance, which could lead to fat accumulation in older individuals. Testosterone might disrupt the mechanisms that protect against adiposity and hyperphagia and represent a risk factor for excessive body weight and obesity, especially in older females.
(徳島大学機関リポジトリ): ● Metadata: 112453
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.steroids.2017.08.014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28893558; ● Search Scopus @ Elsevier (PMID): 28893558; ● Search Scopus @ Elsevier (DOI): 10.1016/j.steroids.2017.08.014

(徳島大学機関リポジトリ: 112453, DOI: 10.1016/j.steroids.2017.08.014, PubMed: 28893558) Takeshi Iwasa, Toshiya Matsuzaki, Yiliyasi Mayila, Kiyohito Yano and Minoru Irahara :
Developmental changes in hypothalamic SF-1, POMC, and ERα mRNA expression and their sensitivity to fasting in male and female rats.,
Endocrine Journal, Vol.64, No.12, 1157-1163, 2017.

(要約): Estrogen plays pivotal roles in body weight regulation through its effects on central estrogen receptor-α (ERα) expression. ERα is found on neurons that express the hypothalamic anorexigenic factors steroidogenic factor-1 (SF-1) and pro-opiomelanocortin (POMC) mediate these effects of estrogen. As the gonadal hormonal milieu is drastically altered during the developmental period, the expression levels of SF-1 and POMC might also change during this period. In this study, we showed that hypothalamic SF-1 and ERα mRNA expression did not change during the neonatal to pre-pubertal period (from postnatal day 10 to 30), and there were no significant differences in the hypothalamic mRNA expression levels of these molecules between males and females at any examined age. On the other hand, hypothalamic POMC mRNA expression and the serum estradiol (E2) level increased during development in both males and females. Significant positive correlations were detected between the serum E2 level and hypothalamic POMC mRNA expression in both males and females. Hypothalamic ERα and POMC mRNA expression were decreased by fasting in male rats at all examined ages, whereas fasting had no effect on hypothalamic ERα or POMC mRNA expression in the female rats. These results indicate that the regulatory system involving E2 and hypothalamic POMC expression might already be established in the neonatal period and that the roles of POMC and ERα in body weight regulation during development might differ slightly between males and females.
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ17-0140
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28883210; ● Search Scopus @ Elsevier (PMID): 28883210; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ17-0140

(DOI: 10.1507/endocrj.EJ17-0140, PubMed: 28883210) Eri Takiguchi, Masato Nishimura, Ayuka Mineda, Takako Kawakita, Akiko Abe and Minoru Irahara :
Growth inhibitory effect of the Src inhibitor dasatinib in combination with anticancer agents on uterine cervical adenocarcinoma cells.,
Experimental and Therapeutic Medicine, Vol.14, No.5, 4293-4299, 2017.

(要約): Uterine cervical adenocarcinoma has a poor clinical prognosis when compared with squamous cell carcinoma. Therefore, the development of new treatment strategies for uterine cervical adenocarcinoma is necessary. Src is a proto-oncogene that is important in cancer progression. Dasatinib is a Src inhibitor that has been reported to be effective when used in combination with anticancer drugs. The present study aimed to confirm Src expression in human cervical adenocarcinoma cell lines and to determine the mechanism underlying the inhibitory effect of dasatinib on Src signaling . Western blot analysis was performed to investigate Src expression in cervical adenocarcinoma cell lines (HeLa and TCO-2 cells). The cells were cultured for 48 h with the addition of different concentrations of anticancer drugs (paclitaxel or oxaliplatin). Viable cell count was measured using a colorimetric (WST-1) assay. The concentrations of anticancer agents were fixed according to the results obtained, and the same experiments were performed using the drugs in combination with dasatinib at various concentrations to determine the concentrations that significantly affected the number of viable cells. The presence or absence of apoptosis was investigated using a caspase-3/7 assay. Signal transduction in each cell line was examined using western blotting. Src was activated in the two cell lines, and cell proliferation was significantly suppressed by each anticancer drug in combination with 10 µM dasatinib. Caspase-3/7 activity was also increased and Src signaling was suppressed by each anticancer drug in combination with dasatinib. In conclusion, Src is overexpressed in cervical adenocarcinoma cell lines, and dasatinib inhibits intracellular Src signaling and causes apoptosis. The results of the present study suggest that Src may be targeted in novel therapeutic strategies for cervical adenocarcinoma.
(徳島大学機関リポジトリ): ● Metadata: 112973
(出版サイトへのリンク): ● Publication site (DOI): 10.3892/etm.2017.5061
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29067110; ● Search Scopus @ Elsevier (PMID): 29067110; ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2017.5061

(徳島大学機関リポジトリ: 112973, DOI: 10.3892/etm.2017.5061, PubMed: 29067110) Minoru Irahara, Tadashi Kimura and Hironori Sugino :
Japan Society for Reproductive Medicine RMB Best Paper Award.,
Reproductive Medicine and Biology, Vol.16, No.3, 239, 2017.

(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12043
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259473; ● Search Scopus @ Elsevier (PMID): 29259473; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12043

(DOI: 10.1002/rmb2.12043, PubMed: 29259473) Toshiya Matsuzaki, Tsutomu Douchi, Toshimichi Oki, Osamu Ishihara, Ryugo Okagaki, Takeshi Kajihara, Midori Tamura, Fumikazu Kotsuji, Kimihisa Tajima, Mika Kawano, Bunpei Ishizuka and Minoru Irahara :
Weight reduction using a formula diet recovers menstruation in obese patients with an ovulatory disorder.,
Reproductive Medicine and Biology, Vol.16, No.3, 268-275, 2017.

(要約): To determine the effectiveness of a formula diet in weight reduction and the recovery of menstruation in obese patients with ovulatory disorders. After the enrollment of 39 obese women with ovulatory disorders, they replaced one or two of their three normal meals with a microdiet (MD) (240 kcal/meal) for 24 weeks. Physical, endocrinological, and biochemical tests were conducted before and at 12 and 24 weeks of the study. Of the 39 women enrolled, 26 were not taking clomiphene. They were divided into three groups according to their body weight outcomes and then analyzed for menstruation recovery. A weight reduction of ≥5% was observed in 31 (81.5%) of the 39 women. There were significant decreases in the body weight and Body Mass Index during the study. Menstruation returned in 18 (69%) of the 26 patients without clomiphene treatment, with the recovery being significantly more prevalent in the groups (totally 81.0%) that exhibited a 5%-10% weight reduction and ≥10% weight reduction, compared to the group with a <5% weight reduction. The use of a formula diet effectively reduced the patients' body weight and led to the recovery of menstruation in these obese patients with ovulatory disorders.
(徳島大学機関リポジトリ): ● Metadata: 112966
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12034
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259477; ● Summary page in Scopus @ Elsevier: 2-s2.0-85022187256

(徳島大学機関リポジトリ: 112966, DOI: 10.1002/rmb2.12034, PubMed: 29259477, Elsevier: Scopus) T Tatsumi, S C. Jwa, Akira Kuwahara, Minoru Irahara, T Kubota and H Saito :
Pregnancy and neonatal outcomes following letrozole use in frozen-thawed single embryo transfer cycles.,
Human Reproduction, Vol.32, No.6, 1244-1248, 2017.

(要約): Are pregnancy and neonatal outcomes following letrozole use comparable with natural and HRT cycles in patients undergoing single frozen-thawed embryo transfer (FET)? Letrozole use was significantly associated with higher rates of clinical pregnancy, clinical pregnancy with fetal heart beat and live birth, and with a lower rate of miscarriage, compared with natural and HRT cycles. Letrozole is the most commonly used aromatase inhibitor for mild ovarian stimulation in ART. However, the effect of letrozole on pregnancy and neonatal outcomes in FET are not well known. A retrospective cohort study was conducted using data from the Japanese national ART registry between 2012 and 2013. A total of 110 722 single FET cycles with letrozole (n = 2409), natural (n = 41 470) or HRT cycles (n = 66 843) were included. The main outcomes were the rates of clinical pregnancy, clinical pregnancy with fetal heart beat, miscarriage and live birth. Adjusted odds ratios and relative risks (RRs) were calculated using a generalized estimating equation adjusting for correlations within clinics. The rates of clinical pregnancy, clinical pregnancy with fetal heart beat, and live birth were significantly higher, while the rate of miscarriage was significantly lower in the letrozole group compared with the natural and HRT groups. In blastocyst stage transfers, the adjusted RRs for clinical pregnancy with fetal heart beat of letrozole compared with natural and HRT cycles were 1.48 (95% CI: 1.41-1.55) and 1.62 (95% CI: 1.54-1.70), respectively. Similarly, the adjusted RRs of letrozole for miscarriage compared with natural and HRT cycles were 0.91 (95% CI: 0.88-0.93) and 0.84 (95% CI: 0.82-0.87), respectively. Neonatal outcomes were mostly similar in letrozole, natural and HRT cycles. Important limitations of this study included the lack of information concerning the reasons for selecting the specific FET method, parity, the number of previous ART failures, embryo quality and the dose and duration of letrozole intake. These results suggest that letrozole use may improve clinical pregnancy, clinical pregnancy with fetal heart beat, and live births and reduce the risk of miscarriage in patients undergoing single FET cycles. No external funding was used for this study. There are no conflicts of interest. Not applicable.
(キーワード): Abortion, Spontaneous / Aromatase Inhibitors / Blastocyst / Cohort Studies / Cryopreservation / Female / Fertility Agents, Female / Hormone Replacement Therapy / Humans / Infant, Newborn / Infertility, Female / Japan / Live Birth / Male / Nitriles / Ovulation Induction / Pregnancy / Pregnancy Rate / Registries / Retrospective Studies / Risk / Single Embryo Transfer / Triazoles
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/humrep/dex066
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28398491; ● Search Scopus @ Elsevier (PMID): 28398491; ● Search Scopus @ Elsevier (DOI): 10.1093/humrep/dex066

(DOI: 10.1093/humrep/dex066, PubMed: 28398491) Mikio Yamasaki, Akira Kuwahara, Takeshi Iwasa, Yuri Yamamoto, Yuka Taniguchi, Yuya Yano, Sumika Matsui, Toshiya Matsuzaki and Minoru Irahara :
Development-related changes in the expression of the ovarian Kiss1 and Kiss1r genes and their sensitivity to human chorionic gonadotropin in prepubertal female rats.,
The Journal of Reproduction and Development, Vol.63, No.4, 409-414, 2017.

(要約): Kisspeptin, which is encoded by the Kiss1 gene, and its receptor, the G protein-coupled receptor 54 (Kiss1r), play important roles in the regulation of reproductive functions in mammals. Several studies have shown that the Kiss1 and Kiss1r genes are expressed in the rat, primate, and human ovaries, and that the ovarian kisspeptin system plays a pivotal role in ovulation at the proestrous stage in adulthood. The purpose of this study was to evaluate development-related changes in the expression of ovarian Kiss1 and Kiss1r genes and in kisspeptin levels, and to identify the regulatory factors for these genes during the prepubertal period. The serum kisspeptin level was also measured to examine whether ovarian kisspeptin affects serum kisspeptin levels. Variations in the ovarian Kiss1 and Kiss1r mRNA levels were observed during the prepubertal period in female rats, with levels peaking around postnatal days 20 and 15, respectively. Nevertheless, the ovarian kisspeptin content per total protein level was stably maintained. Serum kisspeptin levels at postnatal days 30 and 35 were higher than those at earlier postnatal days. The pattern of the ovarian Kiss1 mRNA levels was similar to that of the serum luteinizing hormone (LH) levels, and the ovarian Kiss1 mRNA level increased after injection with human chorionic gonadotropin (HCG) on postnatal day 20, but not on postnatal days 10 and 30. These data indicate that ovarian Kiss1 and Kiss1r mRNA levels are increased on postnatal days 20 and 15, respectively, and that changes in the serum LH level and the ovarian sensitivity to LH may be involved in the alteration of ovarian Kiss1 mRNA levels.
(キーワード): Animals / Chorionic Gonadotropin / Female / Gene Expression Regulation, Developmental / Kisspeptins / Ovary / Rats / Rats, Sprague-Dawley / Receptors, Kisspeptin-1
(徳島大学機関リポジトリ): ● Metadata: 112975
(出版サイトへのリンク): ● Publication site (DOI): 10.1262/jrd.2016-179
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28552864; ● Search Scopus @ Elsevier (PMID): 28552864; ● Search Scopus @ Elsevier (DOI): 10.1262/jrd.2016-179

(徳島大学機関リポジトリ: 112975, DOI: 10.1262/jrd.2016-179, PubMed: 28552864) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Rie Yanagihara, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Yiliyasi Mayila, Akira Kuwahara and Minoru Irahara :
The effects of chronic testosterone administration on body weight, food intake, and adipose tissue are changed by estrogen treatment in female rats.,
Hormones and Behavior, Vol.93, 53-61, 2017.

(要約): In females, estrogens play pivotal roles in preventing excess body weight (BW) gain. On the other hand, the roles of androgens in female BW, appetite, and energy metabolism have not been fully examined. We hypothesized that androgens' effects on food intake (FI) and BW regulation change according to the estrogens' levels. To evaluate this hypothesis, the effects of chronic testosterone administration in ovariectomized (OVX) female rats with or without estradiol supplementation were examined in this study. Chronic testosterone administration decreased BW, FI, white adipose tissue (WAT) weight, and adipocyte size in OVX rats, whereas it increased BW, WAT weight, and adipocyte size in OVX with estradiol-administered rats. In addition, chronic testosterone administration increased hypothalamic CYP19a1 mRNA levels in OVX rats, whereas it did not alter CYP19a1 mRNA levels in OVX with estradiol-administered rats, indicating that conversion of testosterone to estrogens in the hypothalamus may be activated in testosterone-administered OVX rats. Furthermore, chronic testosterone administration decreased hypothalamic TNF-α mRNA levels in OVX rats, whereas it increased hypothalamic IL-1β mRNA levels in OVX with estradiol-administered rats. On the other hand, IL-1β and TNF-α mRNA levels in visceral and subcutaneous WAT and liver were not changed by chronic testosterone administration in both groups. These data indicate that the effects of chronic testosterone administration on BW, FI, WAT weight, and adipocyte size were changed by estradiol treatment in female rats. Testosterone has facilitative effects on BW gain, FI, and adiposity under the estradiol-supplemented condition, whereas it has inhibitory effects in the non-supplemented condition. Differences in the responses of hypothalamic factors, such as aromatase and inflammatory cytokines, to testosterone might underlie these opposite effects.
(キーワード): Adipose Tissue / Adiposity / Animals / Body Weight / Eating / Estradiol / Estrogens / Female / Hypothalamus / Ovariectomy / Rats / Rats, Wistar / Testosterone / Weight Gain
(徳島大学機関リポジトリ): ● Metadata: 112456
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.yhbeh.2017.05.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28522306; ● Search Scopus @ Elsevier (PMID): 28522306; ● Search Scopus @ Elsevier (DOI): 10.1016/j.yhbeh.2017.05.008

(徳島大学機関リポジトリ: 112456, DOI: 10.1016/j.yhbeh.2017.05.008, PubMed: 28522306) Takako Kawakita, Masato Nishimura, Eri Takiguchi, Akiko Abe and Minoru Irahara :
Cytotoxic effects of 15-deoxy-Δ12,14-prostaglandin J2 alone and in combination with dasatinib against uterine sarcoma .,
Experimental and Therapeutic Medicine, Vol.13, No.6, 2939-2945, 2017.

(要約): Effective chemotherapeutic strategies for uterine sarcoma are lacking; existing therapies achieve poor response rates. Previous studies have identified the prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) as a potential anticancer treatment; however, its effectiveness in uterine sarcoma has not been examined. Furthermore, the molecular mechanisms underlying the cytotoxic mechanism of 15d-PGJ2 remain unclear. Here, we evaluated the effects of 15d-PGJ2 alone and in combination with the tyrosine kinas inhibitor (TKI) dasatinib in uterine sarcoma cell lines (MES-SA, MES-SA/DX5 and SKN). 15d-PGJ2 inhibited cell growth and increased apoptosis. Western blotting demonstrated that 15d-PGJ2 treatment increased MEK and ERK phosphorylation, and decreased levels of phosphorylated AKT. Dasatinib in combination with 15d-PGJ2 significantly reduced cell proliferation compared with 15d-PGJ2 alone, and repressed both the AKT and MAPK pathways. The cell growth inhibition rate in the PGJ2 was 21.5±12.0, 35.3±5.4 and 28.3±4.2%, respectively (MES-SA, MES-SA/DX5 and SKN cell lines) and the cell growth inhibition rate in the combination therapy was significantly higher compared with 15d-PGJ2 alone (MES-SA; 64.2±0.8, MES-SA/DX5;23.9±8.2 and SKN; 41.4±17.6%). The PGJ2 IC determined by MTT assay was 27.41,10.46 and 17.38 µmol/l, respectively (MES-SA, MES-SA/DX5 and SKN cell lines) and the dasatinib IC was 6.68,17.30 and 6.25 µmol/l, respectively. Our findings demonstrate that 15d-PGJ2 suppresses proliferation by inactivating the AKT pathway in uterine sarcoma. Furthermore, combining 15d-PGJ2 with dasatinib produced a synergistic effect on cancer cell inhibition by repressing 15d-PGJ2-mediated activation of MAPK signaling, and further repressing AKT signaling. These results suggest that 15d-PGJ2 could be used in combination with dasatinib as a potential therapeutic approach for uterine sarcoma.
(徳島大学機関リポジトリ): ● Metadata: 112972
(出版サイトへのリンク): ● Publication site (DOI): 10.3892/etm.2017.4346
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28587364; ● Search Scopus @ Elsevier (PMID): 28587364; ● Search Scopus @ Elsevier (DOI): 10.3892/etm.2017.4346

(徳島大学機関リポジトリ: 112972, DOI: 10.3892/etm.2017.4346, PubMed: 28587364) Minoru Irahara, Akira Kuwahara, Takeshi Iwasa, Tomonori Ishikawa, Osamu Ishihara, Koji Kugu, Rintaro Sawa, Kouji Banno and Hidekazu Saito :
Assisted reproductive technology in Japan: a summary report of 1992-2014 by the Ethics Committee, Japan Society of Obstetrics and Gynecology.,
Reproductive Medicine and Biology, Vol.16, No.2, 126-132, 2017.

(要約): The Japan Society of Obstetrics and Gynecology implemented a registry report system for the clinical practice of assisted reproductive technology in 1986. The aggregated results from 1992 to 2014 are reported herein. The total number of registered treatments was 393 745 cycles, of which 66 550 were pregnancy cycles and 46 008 were cycles with a live birth. Compared to the number of registered treatments in 2008, when the cycle-based registry was newly introduced, there was a 2.07-fold increase in the total number of treatments and a 2.25-fold increase in the number of cycles with a live birth. As the average age of patients who receive assisted reproductive technology has become markedly higher year by year, the most common age of those patients who received assisted reproductive technology in 2014 was 40 years. The total numbers of both assisted reproductive technology treatments and assisted reproductive technology live births are likely to be higher in the future. In addition, the trend toward aging patients seems to be continuing into the future.
(徳島大学機関リポジトリ): ● Metadata: 112963
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259459; ● Search Scopus @ Elsevier (PMID): 29259459; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12014

(徳島大学機関リポジトリ: 112963, DOI: 10.1002/rmb2.12014, PubMed: 29259459) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano and Minoru Irahara :
Gonadotropin-Inhibitory Hormone Plays Roles in Stress-Induced Reproductive Dysfunction.,
Frontiers in Endocrinology, Vol.8, 62, 2017.

(要約): Physical and psychological stressors suppress hypothalamic-pituitary-gonadal axis activity and sexual behavior and consequently induce reproductive dysfunction. Recently, it has been shown that gonadotropin-inhibitory hormone (GnIH), also called RFamide-related peptide 3 (RFRP) in mammals, which is a potent inhibitory regulator of gonadotropin-releasing hormone (GnRH) and gonadotropin, is involved in stress-induced reproductive dysfunction. GnIH/Rfrp (the gene coding RFRP-3) expression and activity are increased by psychological and immune stress, and this alteration suppresses GnRH and gonadotropin secretion. Glucocorticoid acts as a mediator that interacts between stress and hypothalamic GnIH/RFRP-3. GnIH/RFRP-3 also plays important roles in stress-induced suppression of sexual behavior and infertility, and genetic silencing of GnIH/Rfrp completely recovers sexual behavior and fertility. This review summarizes what is currently known about the roles of GnIH in stress-induced reproductive dysfunction.
(徳島大学機関リポジトリ): ● Metadata: 112957
(出版サイトへのリンク): ● Publication site (DOI): 10.3389/fendo.2017.00062
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28424661; ● Search Scopus @ Elsevier (PMID): 28424661; ● Search Scopus @ Elsevier (DOI): 10.3389/fendo.2017.00062

(徳島大学機関リポジトリ: 112957, DOI: 10.3389/fendo.2017.00062, PubMed: 28424661) Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Takeshi Iwasa, Munkhsaikhan Munkhzaya, Kiyohito Yano, Yiliyasi Mayila, Takako Tokui, Rie Yanagihara, Sumika Matsui, Takeshi Katou, Akira Kuwahara and Minoru Irahara :
Clinical outcome of various metformin treatments for women with polycystic ovary syndrome.,
Reproductive Medicine and Biology, Vol.16, No.2, 179-187, 2017.

(要約): Polycystic ovary syndrome (PCOS) is an ovulatory disorder and insulin resistance and diabetes are involved in its pathophysiology. Metformin, an anti-diabetic agent, has been reported to be useful to induce ovulation. Metformin treatment was classified into four types: (1) clomiphene-metformin combination treatment for clomiphene-resistant patients; (2) clomiphene-metformin combination for clomiphene-sensitive patients; (3) clomiphene-metformin combination for naïve patients; and (4) metformin monotherapy. The patients underwent physical, endocrinological, and clinical examinations for their ovulation rates, pregnancy rates, and follicular development. The ovulation rates, pregnancy rates, and single follicular development were not significantly different among the clomiphene-metformin combination treatment groups. In the Body Mass Index (BMI) subanalysis, the pregnancy rate was higher in the BMI≥30 kg/m group than in the other three groups with a BMI of ≤30 kg/m in both cycles and cases. The ovulation rates and pregnancy rates were significantly higher in the group with a fasting insulin of ≥15 μU/mL than in the groups with a fasting insulin of <15 μU/mL in both cycles and cases. Clomiphene-metformin combination treatment appears to be useful, at least for clomiphene-resistant patients, and a BMI of >30 kg/m and a fasting insulin of ≥15 μU/mL appear to be predictors of a good result with this treatment.
(徳島大学機関リポジトリ): ● Metadata: 112965
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12026
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259467; ● Search Scopus @ Elsevier (PMID): 29259467; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12026

(徳島大学機関リポジトリ: 112965, DOI: 10.1002/rmb2.12026, PubMed: 29259467) Toshiya Matsuzaki, Munkhsaikhan Munkhzaya, Takeshi Iwasa, Altankhuu Tungalagsuvd, Kiyohito Yano, Yiliyasi Mayila, Rie Yanagihara, Takako Tokui, Takeshi Katou, Akira Kuwahara, Sumika Matsui and Minoru Irahara :
Relationship between serum anti-Mullerian hormone and clinical parameters in polycystic ovary syndrome.,
Endocrine Journal, Vol.64, No.5, 531-541, 2017.

(要約): Polycystic ovary syndrome (PCOS) is an ovulatory disorder that affects 6-10% of women of reproductive age. Serum AMH level may be an additional factor, or surrogate of PCOM, in the diagnostic criteria of PCOS. We evaluated the correlations between the serum AMH level and various endocrine and metabolic features in PCOS using the latest fully automated assay. Serum AMH level was compared between 114 PCOS patient (PCOS group) and 95 normal menstrual cycle women (Control group). Correlations between serum AMH level and various endocrine and metabolic factors were analysed in PCOS group. The serum AMH level was significantly higher in the PCOS group (8.35±8.19 ng/mL) than in the Control group (4.99±3.23 ng/mL). The serum AMH level was independently affected by age and the presence of PCOS on multiple regression analysis. Ovarian volume per ovary (OPVO) showed the strongest positive correlation (r=0.62) with the serum AMH level among related factors. On receiver operating characteristic (ROC) curve analysis, the cut-off value of AMH for the diagnosis of PCOS was 7.33 ng/mL, but this value did not have high efficacy (sensitivity 44.7%, specificity 76.8%). A cut-off value of 10 ng/mL had a high specificity of 92.6%, although the sensitivity was low (24.6%). The serum AMH level was elevated and reflected ovarian size in PCOS patients. The serum AMH level could be a surrogate for ultrasound findings of the ovaries in PCOS and might be useful for estimating ovarian findings without transvaginal ultrasound in the diagnosis of PCOS.
(キーワード): Adolescent / Adult / Age Factors / Anti-Mullerian Hormone / Female / Humans / Organ Size / Ovary / Polycystic Ovary Syndrome / Sensitivity and Specificity / Ultrasonography / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ16-0501
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28381699; ● Search Scopus @ Elsevier (PMID): 28381699; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ16-0501

(DOI: 10.1507/endocrj.EJ16-0501, PubMed: 28381699) Sumika Matsui, Toshiyuki Yasui, Kana Kasai, Kaoru Keyama, Kanako Yoshida, Takeshi Katou, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Sex hormone-binding globulin and antithrombin III activity in women with oral ultra-low-dose estradiol.,
Journal of Obstetrics and Gynaecology, Vol.37, No.5, 627-632, 2017.

(要約): Oral oestrogen increases the risk of venous thromboembolism (VTE) and increases production of sex hormone-binding globulin (SHBG) in a dose-dependent manner. SHBG has been suggested to be involved in venous thromboembolism. We examined the effects of oral ultra-low-dose oestradiol on circulating levels of SHBG and coagulation parameters, and we compared the effects to those of transdermal oestradiol. Twenty women received oral oestradiol (500 g) every day (oral ultra-low-dose group) and 20 women received a transdermal patch (50 g) as a transdermal group. In addition, the women received dydrogesterone continuously (5g) except for women who underwent hysterectomy. Circulating SHBG, antithrombin III (ATIII) activity, d-dimer, thrombin-antithrombin complex and plasmin- 2 plasmin inhibitor complex were measured before and 3 months after the start of treatment. SHBG was significantly increased at 3 months in the oral ultra-low-dose group, but not in the transdermal group. However, percent changes in SHBG were not significantly different between the two groups. In both groups, ATIII was significantly decreased at 3 months. In conclusion, even ultra-low-dose oestradiol orally increases circulating SHBG level. However, the magnitude of change in SHBG caused by oral ultra-low-dose oestradiol is small and is comparable to that caused by transdermal oestradiol. Impact statement Oral oestrogen replacement therapy increases production of SHBG which may be related to increase in VTE risk. However, the effect of oral ultra-low-dose oestradiol on SHBG has not been clarified. Even ultra-low-dose oestradiol orally increases circulating SHBG levels, but the magnitude of change in SHBG caused by oral ultra-low-dose oestradiol is small and is comparable to that caused by transdermal oestradiol. VTE risk in women receiving oral ultra-low-dose oestradiol may be comparable to that in women receiving transdermal oestradiol.
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/01443615.2017.1285275
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28317410; ● Search Scopus @ Elsevier (PMID): 28317410; ● Search Scopus @ Elsevier (DOI): 10.1080/01443615.2017.1285275

(DOI: 10.1080/01443615.2017.1285275, PubMed: 28317410) Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Yiliyasi Mayila, Akira Kuwahara and Minoru Irahara :
The effects of prenatal undernutrition and a high-fat postnatal diet on central and peripheral orexigenic and anorexigenic factors in female rats.,
Endocrine Journal, Vol.64, No.6, 597-604, 2017.

(要約): Prenatal undernutrition and postnatal overnutrition increase the risk of some peripheral and central metabolic disorders in adulthood. We speculated that disturbances of appetite/metabolic regulatory factors might already have been established in the early stages of life and contribute to obesity later in life. The effects of a high-fat diet on the levels of peripheral and central appetite/metabolic regulatory factors were compared between the offspring of normally nourished dams and those of undernourished dams in the peri-pubertal period. In the offspring of the normally nourished dams (control), the consumption of the high-fat diet resulted in lower hypothalamic mRNA levels of orexigenic factors (neuropeptide Y (NPY) and prepro-orexin (pporexin)), whereas no such changes were seen in the offspring of the undernourished dams (subjected to intrauterine growth restriction). These results indicate that in high-energy conditions either the adaptive response does not function properly or has not been established in the offspring of undernourished dams. Because NPY and pporexin are negatively regulated by leptin, these findings suggest that in the intrauterine growth restriction group, the leptin resistance of hypothalamic functions, which is usually caused by diet-induced obesity in adulthood, had already been established in the peri-pubertal period.
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ16-0593
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28321032; ● Search Scopus @ Elsevier (PMID): 28321032; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ16-0593

(DOI: 10.1507/endocrj.EJ16-0593, PubMed: 28321032) Yuu Tanaka, Akira Kuwahara, Kenjiro Ushigoe, Yuya Yano, Yuka Taniguchi, Yuri Yamamoto, Toshiya Matsuzaki, Toshiyuki Yasui and Minoru Irahara :
Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis.,
Reproductive Medicine and Biology, Vol.16, No.2, 157-165, 2017.

(要約): Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined. Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax). The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration. These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
(徳島大学機関リポジトリ): ● Metadata: 113212
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/rmb2.12022
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29259464; ● Search Scopus @ Elsevier (PMID): 29259464; ● Search Scopus @ Elsevier (DOI): 10.1002/rmb2.12022

(徳島大学機関リポジトリ: 113212, DOI: 10.1002/rmb2.12022, PubMed: 29259464) Mikio Sugano, Homare Yoshida, Hirotsugu Kurobe, Hiroki Arase, Hajime Kinoshita, Takashi Kitaichi, Noriko Sugasawa, Soichiro Nakayama, Kazuhisa Maeda, Minoru Irahara and Tetsuya Kitagawa :
Effects of Transplanted Human Cord Blood- Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution.,
The Journal of Medical Investigation : JMI, Vol.64, No.1,2, 43-49, 2017.

(要約): To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Medial thickness, RV/S+LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH. J. Med. Invest. 64: 43-49, February, 2017.
(徳島大学機関リポジトリ): ● Metadata: 114302
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.64.43
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28373627; ● Search Scopus @ Elsevier (PMID): 28373627; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.64.43

(徳島大学機関リポジトリ: 114302, DOI: 10.2152/jmi.64.43, PubMed: 28373627) Takashi Kaji, Yasunobu Hayabuchi, Kazuhisa Maeda, Soichiro Nakayama and Minoru Irahara :
Prenatal assessment of coronary artery anatomy using color Doppler in cases of D-transposition of the great arteries: Case reports,
The Journal of Obstetrics and Gynaecology Research, Vol.43, No.2, 397-402, 2017.

(要約): Abnormal coronary artery (CA) anatomy is common in cases of D-transposition of the great arteries (TGA) and can be a significant risk factor during the arterial switch operation. Here, we report three cases of TGA in which CA anatomy was assessed prenatally using color Doppler imaging. All CA, except the left circumflex CA in one case, were identified. CA anatomy was completely correctly diagnosed in one of our three cases. In the two remaining cases, the left circumflex CA could not be visualized in one patient, and the origin of the left anterior descending CA was not correctly diagnosed in the other. We found that prenatal assessment of CA anatomy using color Doppler in TGA was feasible, but the diagnostic accuracy was limited. We anticipate that more experience with the advancing technology will improve accuracy.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jog.13214
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28150404; ● Search Scopus @ Elsevier (PMID): 28150404; ● Search Scopus @ Elsevier (DOI): 10.1111/jog.13214

(DOI: 10.1111/jog.13214, PubMed: 28150404) Kazuhisa Maeda, Takashi Kaji, Souichiro Nakayama, Daichi Nakaoku, M Murakami, A Kondo, M Morine, Kenji Hinokio and Minoru Irahara :
Pregnancies with factor V deficiency: a case report and review of the literature.,
Clinical and Experimental Obstetrics & Gynecology, Vol.44, No.2, 299-300, 2017.

(要約): A 30-year-old Japanese nulliparous woman visited for pregnancy at 33 weeks with a massive ovarian tumor located in the pouch of Douglas. By preoperative screening, her prothrombin time (PT) and activated partial thromboplastin time (APTT) were prolonged, and her FV activity was significantly decreased to 4.8%. After prophylactic FFP 20 ml/kg was administered and her FV factor was 19.3%, cesarean delivery was performed, and her perioperative course was uneventful. One year later, she underwent a dilatation and evacuation because of a missed abortion, although prophylactic FFP was not administered. During a third pregnancy, after prophylactic FFP 20 ml/kg was administered and FV activity increased to 21.1%, elective cesarean delivery was performed, and her postoperative course was uneventful. For surgical therapy or delivery, the goal of therapy is to maintain FV activity above 20%. It is particularly useful to administer prophylactic FFP.
(キーワード): Cesarean Section / Factor V Deficiency / Female / Humans / Obstetric Labor Complications / Ovarian Neoplasms / Ovariectomy / Partial Thromboplastin Time / Plasma / Pregnancy / Pregnancy Complications, Hematologic / Pregnancy Complications, Neoplastic / Pregnancy Outcome / Treatment Outcome
(出版サイトへのリンク): ● Publication site (DOI): 10.12891/ceog3351.2017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29746045; ● Summary page in Scopus @ Elsevier: 2-s2.0-85017188054

(DOI: 10.12891/ceog3351.2017, PubMed: 29746045, Elsevier: Scopus) T Tatsumi, S C. Jwa, Akira Kuwahara, Minoru Irahara, T Kubota and H Saito :
No increased risk of major congenital anomalies or adverse pregnancy or neonatal outcomes following letrozole use in assisted reproductive technology.,
Human Reproduction, Vol.32, No.1, 125-132, 2016.

(要約): Does letrozole use increase the risk of major congenital anomalies and adverse pregnancy and neonatal outcomes in fresh, single-embryo transfer? Letrozole significantly decreases the risk of miscarriage and does not increase the risk of major congenital anomalies or adverse pregnancy or neonatal outcomes compared with natural cycles in patients undergoing ART. Letrozole is the most commonly used aromatase inhibitor for mild ovarian stimulation in ART. However, its safety in terms of pregnancy and neonatal outcomes is unclear. This retrospective cohort study used data from the Japanese national ART registry from 2011 to 2013. A total of 3136 natural cycles and 792 letrozole-induced cycles associated with fresh, single-embryo transfer and resulting in a clinical pregnancy were included in the analysis. The main pregnancy outcomes were miscarriage, ectopic pregnancy and still birth, and the neonatal outcomes were preterm delivery, low birth weight, small/large for gestational age and major congenital anomalies. Terminated pregnancies were included in the analysis of major congenital anomalies. Odds ratios (ORs) and 95% CIs were calculated using multivariate logistic regression analysis adjusted for maternal age and calendar year. The risk of miscarriage was significantly lower in women administered letrozole (adjusted OR [aOR], 0.37, 95% CI, 0.30-0.47, P < 0.001). There was no significant difference in the overall risk of major congenital anomalies between the two groups (natural cycle 1.5% vs letrozole 1.9%, aOR, 1.24, 95% CI, 0.64-2.40, P = 0.52), and no increased risk for any specific organ system. Subgroup analysis demonstrated that the risk of major congenital anomalies was not increased in patients who underwent either in vitro fertilization or ICSI, or in those who received early cleavage stage or blastocyst embryo transfer. All other pregnancy and neonatal outcomes were comparable between the two groups. Despite the large sample size, we were only able to rule out the possibility that letrozole might cause large increases in birth-defect risks in ART patients. The results suggest that letrozole stimulation reduces the risk of miscarriage, with no increase in the risk of major congenital anomalies or adverse pregnancy or neonatal outcomes compared with natural cycles in women undergoing ART. Letrozole may thus be a safe option for mild ovarian stimulation. None. Not applicable.
(キーワード): Abnormalities, Drug-Induced / Abortion, Spontaneous / Adult / Aromatase Inhibitors / Female / Fertilization in Vitro / Humans / Infant, Newborn / Maternal Age / Nitriles / Ovulation Induction / Pregnancy / Pregnancy Outcome / Retrospective Studies / Risk / Triazoles
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/humrep/dew280
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27821708; ● Search Scopus @ Elsevier (PMID): 27821708; ● Search Scopus @ Elsevier (DOI): 10.1093/humrep/dew280

(DOI: 10.1093/humrep/dew280, PubMed: 27821708) Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Takeshi Iwasa, Munkhsaikhan Munkhzaya, Rie Yanagihara, Takako Tokui, Kiyohito Yano, Yiliyasi Mayila, Takeshi Katou, Akira Kuwahara, Sumika Matsui and Minoru Irahara :
Kisspeptin mRNA expression is increased in the posterior hypothalamus in the rat model of polycystic ovary syndrome.,
Endocrine Journal, Vol.64, No.1, 7-14, 2016.

(要約): Hypersecretion of luteinizing hormone (LH) is a common endocrinological finding of polycystic ovary syndrome (PCOS). This derangement might have a close relationship with hypothalamic kisspeptin expression that is thought to be a key regulator of gonadotropin-releasing hormone (GnRH). We evaluated the relationship between the hypothalamic-pituitary-gonadal axis (HPG axis) and kisspeptin using a rat model of PCOS induced by letrozole. Letrozole pellets (0.4 mg/day) and control pellets were placed subcutaneously onto the backs of 3-week-old female Wistar rats. Body weight, vaginal opening and vaginal smear were checked daily. Blood and tissues of ovary, uterus and brain were collected at 12-weeks of age. An hypothalamic block was cut into anterior and posterior blocks, which included the anteroventral periventricular nucleus (AVPV) and the arcuate nucleus (ARC), respectively, in order to estimate hypothalamic kisspeptin expression in each area. The letrozole group showed a similar phenotype to human PCOS such as heavier body weight, heavier ovary, persistent anovulatory state, multiple enlarged follicles with no corpus luteum and higher LH and testosterone (T) levels compared to the control group. Kisspeptin mRNA expression in the posterior hypothalamic block including ARC was higher in the letrozole group than in the control group although its expression in the anterior hypothalamic block was similar between groups. These results suggest that enhanced KNDy neuron activity in ARC contributes to hypersecretion of LH in PCOS and might be a therapeutic target to rescue ovulatory disorder of PCOS in the future.
(キーワード): Animals / Disease Models, Animal / Female / Gene Expression Regulation / Humans / Hypothalamus, Posterior / Kisspeptins / Nitriles / Polycystic Ovary Syndrome / RNA, Messenger / Rats / Rats, Wistar / Triazoles / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ16-0282
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27665725; ● Search Scopus @ Elsevier (PMID): 27665725; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ16-0282

(DOI: 10.1507/endocrj.EJ16-0282, PubMed: 27665725) Junpei Yamamoto, Midori Omura, Koichiro Tuchiya, Mayumi Hidaka, Akira Kuwahara, Minoru Irahara, Tamotsu Tanaka and Akira Tokumura :
Preferable existence of polyunsaturated lysophosphatidic acids in human follicular fluid from patients programmed with in vitro fertilization.,
Prostaglandins & Other Lipid Mediators, Vol.126, 16-23, 2016.

(要約): Lysophosphatidic acid (LPA) exerts diverse physiological effects on various types of animal cells, including reproductive cells, through its binding to six LPA receptors. We previously found that LPA promoted maturation of the nucleus and cytoplasm of mouse and hamster oocytes surrounded by cumulus cells in vitro. Using gas-liquid chromatography, we previously reported detection of several species of LPA by analyzing the fatty acid methyl esters derived from thin layer chromatography-purified LPA in lipid extract from incubated follicular fluids programmed with in vitro fertilization. In this study using liquid chromatography- tandem mass spectrometry, we directly detected high levels of linoleoyl, arachidonoyl, and docosahexaenoyl LPAs in human follicular fluid. This unique molecular species composition of LPA was suggested to be due to a balance between the low LPA-degrading activity and high LPA-producing activity of autotaxin in human follicular fluid. Our results suggest that polyunsaturated LPAs produced by autotaxin in human follicular fluid exert unknown physiological effects on cumulus cells.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.prostaglandins.2016.07.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27421691; ● Summary page in Scopus @ Elsevier: 2-s2.0-84978805217

(DOI: 10.1016/j.prostaglandins.2016.07.008, PubMed: 27421691, Elsevier: Scopus) Takeshi Iwasa, Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Maira Yiliyasi, Takeshi Katou, Akira Kuwahara and Minoru Irahara :
Developmental changes in the hypothalamic mRNA expression levels of PACAP and its receptor PAC1 and their sensitivity to fasting in male and female rats.,
International Journal of Developmental Neuroscience, Vol.52, 33-37, 2016.

(要約): The actions and responses of hypothalamic appetite regulatory and factors change markedly during the neonatal to pre-pubertal period. Pituitary adenylate cyclase-activating polypeptide (PACAP) has been found to play pivotal roles in the regulation of metabolic and nutritional status through its specific receptor PAC1. PACAP/PAC1 have anorectic roles, and their functions are regulated by leptin in adulthood. In the present study, we showed that hypothalamic PACAP mRNA expression decreases during the neonatal to pre-pubertal period (from postnatal day 10-30) in both male and female rats. During this period, hypothalamic PACAP mRNA expression was not affected by 24h fasting in either sex, while the serum leptin levels (leptin is a positive regulator of hypothalamic PACAP expression in adulthood) of both sexes were decreased by fasting. On the other hand, hypothalamic PAC1 mRNA expression did not change during the neonatal to pre-pubertal period in either sex; however, its levels were consistently higher in males than in females. Hypothalamic PAC1 mRNA expression was decreased by 24h fasting in males, but no such changes were observed in females. These results indicate while hypothalamic PACAP expression is sensitive to a negative energy state and the serum leptin level in adulthood, no such relationships are seen in the pre-pubertal period. In addition, we speculate that differences in the gonadal steroidal milieu might induce sexual dimorphism in the basal hypothalamic PAC1 mRNA level and its response to fasting. The mechanisms responsible for and the physiological effects of such changes in hypothalamic PACAP and PAC1 expression during the developmental period remain to be clarified.
(キーワード): Age Factors / Analysis of Variance / Animals / Animals, Newborn / Fasting / Female / Gene Expression Regulation, Developmental / Hypothalamus / Leptin / Male / Pituitary Adenylate Cyclase-Activating Polypeptide / Pregnancy / RNA, Messenger / Rats / Rats, Sprague-Dawley / Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I / Sex Characteristics
(徳島大学機関リポジトリ): ● Metadata: 112450
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2016.05.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27181029; ● Search Scopus @ Elsevier (PMID): 27181029; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2016.05.003

(徳島大学機関リポジトリ: 112450, DOI: 10.1016/j.ijdevneu.2016.05.003, PubMed: 27181029) Sumika Matsui, Toshiyuki Yasui, Kana Kasai, Kaoru Keyama, Takeshi Katou, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Changes of liver enzymes and triglyceride during the menopausal transition in Japanese women.,
Journal of Obstetrics and Gynaecology, Vol.36, No.6, 806-811, 2016.

(要約): We examined detailed changes in liver enzymes as surrogate markers for metabolic syndrome and non-alcoholic fatty liver disease (NAFLD) during the menopausal transition and the associations of liver enzymes with lipid profiles related to risk of metabolic syndrome and endocrinological hormones. We divided 393 women into seven stages by menstrual regularity, follicle-stimulating hormone level and years since menopause. Serum levels of alanine aminotranferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase, lipid parameters, glucose, and endocrinological hormones were measured. Both levels of AST and ALT increased towards early post-menopause. AST remained high in late post-menopause but ALT decreased. The AST/ALT ratio decreased towards late menopausal transition and very early post-menopause and increased thereafter. This ratio was negatively correlated with triglyceride. Significant changes in ALT and AST/ALT ratio during the menopausal transition, which were associated with triglyceride, might be involved in the occurrence of metabolic syndrome and NAFLD in Japanese women.
(キーワード): Adult / Age Factors / Alanine Transaminase / Aspartate Aminotransferases / Blood Glucose / Female / Follicle Stimulating Hormone / Humans / Japan / Lipids / Liver / Menopause / Metabolic Syndrome X / Middle Aged / Non-alcoholic Fatty Liver Disease / Risk Factors / Time Factors / Triglycerides / gamma-Glutamyltransferase
(出版サイトへのリンク): ● Publication site (DOI): 10.3109/01443615.2016.1154516
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27167882; ● Search Scopus @ Elsevier (PMID): 27167882; ● Search Scopus @ Elsevier (DOI): 10.3109/01443615.2016.1154516

(DOI: 10.3109/01443615.2016.1154516, PubMed: 27167882) Takashi Kaji, Kazuhisa Maeda, A Hichijo, Y Takahashi, S Nakayama and Minoru Irahara :
Three-dimensional HDlive rendering of fetal perineum in anorectal atresia.,
Ultrasound in Obstetrics & Gynecology, Vol.47, No.6, 784-785, 2016.

(キーワード): Adult / Anorectal Malformations / Female / Fetus / Humans / Image Processing, Computer-Assisted / Imaging, Three-Dimensional / Pregnancy / Ultrasonography, Prenatal
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/uog.15776
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26434560; ● Search Scopus @ Elsevier (PMID): 26434560; ● Search Scopus @ Elsevier (DOI): 10.1002/uog.15776

(DOI: 10.1002/uog.15776, PubMed: 26434560) Altankhuu Tungalagsuvd, Toshiya Matsuzaki, Takeshi Iwasa, Munkhsaikhan Munkhzaya, Mayila Yiliyasi, Takako Kawami, Takeshi Katou, Akira Kuwahara and Minoru Irahara :
The expression of orexigenic and anorexigenic factors in middle-aged female rats that had been subjected to prenatal undernutrition.,
International Journal of Developmental Neuroscience, Vol.49, 1-5, 2016.

(要約): Fetal growth retardation, which affects short- and long-term fetal brain development, is associated with metabolic, hematological, and thermal disturbances, which can increase the risk of metabolic syndrome later in life. Orexigenic and anorexigenic factors regulate food intake and energy expenditure. We studied how the expression of these factors was affected by food deprivation (FD) in middle-aged female rats that had been subjected to prenatal undernutrition. Eight pregnant rats were divided into two groups, the normal nutrition (NN) (n=4) group and the undernutrition (UN) (n=4) group, which received 50% (approximately 11g) of the daily food intake of the normal nutrition rats from day 13 of pregnancy to delivery. The pups from these dams were defined as the maternal NN (mNN) and maternal UN (mUN) groups, respectively. After weaning, all of the pups were housed and allowed ad libitum access to food and water. At the age of 6 months, both groups of pups were sub-divided into three groups. One group was allowed to consume normal amounts of food (Fed), and the other two groups were subjected to 24h or 48h FD (n=7-8 per group). The rats' serum leptin levels and hypothalamic mRNA expression levels of various orexigenic or anorexigenic factors were measured. In both the mNN and mUN rats, the serum leptin levels of the 24h and 48h FD groups tended to be lower than those of the Fed group, and the serum leptin levels of the 24h FD mUN rats and the Fed mUN rats differed significantly. The hypothalamic neuropeptide Y (NPY) mRNA expression levels of the 24h and 48h FD groups were significantly higher in the mUN rats than in the mNN rats. In addition, among the mUN rats the hypothalamic NPY mRNA expression levels of the 48h FD group were significantly higher than those of the Fed group. In both the mNN and mUN rats, prepro-orexin mRNA expression was lower in the 48h FD group than in the corresponding Fed group. Among the mUN rats, the 48h FD group exhibited significantly lower hypothalamic proopiomelanocortin (POMC) mRNA expression than the Fed group, and a similar tendency was seen among the mNN rats. Among the mNN rats, the 24h FD group displayed significantly higher hypothalamic leptin receptor (OBRb) mRNA levels than the Fed group. However, no such differences were seen among the mUN rats. As a result, the hypothalamic OBRb mRNA expression levels of the mUN rats in the 24h and 48h FD groups were lower than those of the corresponding mNN rat groups. These findings indicate that rats that are subjected to prenatal undernutrition exhibit upregulated expression of orexigenic factors and are more sensitive to FD in middle age, which might increase their risk of developing metabolic disorders in later life.
(徳島大学機関リポジトリ): ● Metadata: 110114
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2015.12.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26702900; ● Summary page in Scopus @ Elsevier: 2-s2.0-84958161611

(徳島大学機関リポジトリ: 110114, DOI: 10.1016/j.ijdevneu.2015.12.002, PubMed: 26702900, Elsevier: Scopus) Kazuki Saito, Toshiya Matsuzaki, Takeshi Iwasa, Mami Miyado, Hidekazu Saito, Tomonobu Hasegawa, Keiko Homma, Eisuke Inoue, Yoshimichi Miyashiro, Toshiro Kubota, Minoru Irahara, Tsutomu Ogata and Maki Fukami :
Steroidogenic pathways involved in androgen biosynthesis in eumenorrheic women and patients with polycystic ovary syndrome.,
The Journal of Steroid Biochemistry and Molecular Biology, Vol.158, 31-37, 2016.

(要約): The conventional Δ5 and Δ4 steroidogenic pathways mediate androgen production in females. While multiple non-conventional pathways to dihydrotestosterone (DHT) have recently been postulated in humans, the functional significance of these pathways remains to be elucidated. The aim of this study was to clarify the origin of androgens in healthy women and in patients with polycystic ovary syndrome (PCOS), a multifactorial disorder characterized by androgen overproduction. We measured 13 steroids in blood samples of 31 eumenorrheic females and 28 PCOS patients using liquid chromatography-tandem mass spectrometry and chemiluminescent enzyme immunoassay. We found that 17-hydroxy (17-OH) progesterone (17-OHP), androstenedione (Δ4A), testosterone, androstanedione, androsterone, and androstanediol levels were higher in the patient group than in the eumenorrheic group, while levels of other steroids were comparable between the two groups. In the eumenorrheic group, DHT levels were correlated with testosterone, androstanedione, and androstanediol. Quantitative correlations were also observed among 17-OH allopregnanolone, androsterone, androstanediol, and DHT, and among Δ4A, androstanedione, androsterone, and androstanediol. In the patient group, DHT levels were correlated with testosterone levels, but not with androstanedione or androstanediol levels. Δ4A and testosterone paralleled 17-OHP. Androstanedione, androsterone, androstanediol, and 17-OH allopregnanolone were quantitatively correlated. In both groups, multivariable linear regression analyses suggested relationships between androsterone and androstanedione, as well as between androsterone and 17-OH allopregnanolone. These results indicate that multiple androgen biosynthesis pathways are operating in eumenorrheic females and PCOS patients. In PCOS patients, excessive androgens are produced primarily via the conventional pathways, while two alternative pathways; i.e., an androstanedione-mediated pathway and a so-called backdoor pathway, likely serve as sources of a weak androgen and potential precursors of DHT.
(キーワード): Adult / Androgens / Chromatography, High Pressure Liquid / Female / Hormones / Humans / Polycystic Ovary Syndrome / Spectrometry, Mass, Electrospray Ionization / Tandem Mass Spectrometry / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jsbmb.2016.02.010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26877255; ● Search Scopus @ Elsevier (PMID): 26877255; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jsbmb.2016.02.010

(DOI: 10.1016/j.jsbmb.2016.02.010, PubMed: 26877255) Takeshi Iwasa, Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Mayila Yiliyasi, Takeshi Katou, Akira Kuwahara and Minoru Irahara :
Prenatal undernutrition results in greater lipopolysaccharide-induced changes in hypothalamic TNF- expression, but does not affect the equivalent changes in the serum levels of luteinizing hormone and testosterone, in adult male rats.,
International Journal of Developmental Neuroscience, Vol.48, 80-83, 2016.

(要約): Immune stress can cause reproductive dysfunction. Some hypothalamic factors such as pro-inflammatory cytokines play pivotal roles in reproductive disorders under immune stress conditions. Recently, it has been reported that prenatal undernutrition affects not only metabolic functions, but also the responses of physiological functions to immune stress in adulthood. In this study, the long-term effects of prenatal undernutrition on the responses of hypothalamic pro-inflammatory cytokine (interleukin (IL)-1, tumor necrosis factor (TNF)-, and IL-6) expression; reproductive endocrine factors; i.e., the serum levels of gonadotropins and testosterone; and hypothalamic kisspeptin expression to lipopolysaccharide (LPS) were examined in male rats. Pregnant rats were divided into two groups; i.e., the normally nourished group and the undernourished (50% food restricted) group. The offspring of the normally nourished mothers (control) and undernourished mothers (the intrauterine growth restriction [IUGR] group) were sub-divided into saline-injected and LPS (500g, i.p.)-injected groups at 10 weeks of age. The rats' hypothalamic pro-inflammatory cytokine levels and serum luteinizing hormone (LH) and testosterone levels were measured and compared between the control and IUGR groups. The hypothalamic pro-inflammatory cytokine mRNA levels of the LPS-injected rats were significantly higher than those of the saline-injected rats in both the control and IUGR groups. The changes in the hypothalamic expression level of TNF-, but not those of the other cytokines, induced in response to LPS were more marked in the IUGR group than in the control group. On the other hand, although the serum LH and testosterone levels of the LPS-injected rats were significantly lower than those of the saline-injected rats in both the control and IUGR groups, their levels did not differ between the control and IUGR groups under the LPS-injected conditions. These results suggest that prenatal undernutrition results in more marked LPS-induced changes in hypothalamic TNF- expression, but does not alter the effects of LPS on the serum levels of LH or testosterone, in adult male rats.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2015.12.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26674468; ● Search Scopus @ Elsevier (PMID): 26674468; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2015.12.001

(DOI: 10.1016/j.ijdevneu.2015.12.001, PubMed: 26674468) 立花綾香, 加地剛, 七條あつ子, 髙橋洋平, 中山聡一郎, 中川竜二, 前田和寿, 早渕康信, 香美祥二, 苛原稔 :
胎児期から類洞交通を確認できた純型肺動脈閉鎖の一例,
日本周産期・新生児医学会雑誌, Vol.52, No.1, 155-159, 2016年.

(徳島大学機関リポジトリ): ● Metadata: 113945
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050566774709068288

(徳島大学機関リポジトリ: 113945, CiNii: 1050566774709068288) Kazumi Takeshima, Seung Chik Jwa, Hidekazu Saito, Aritoshi Nakaza, Akira Kuwahara, Osamu Ishihara, Minoru Irahara, Fumiki Hirahara, Yasunori Yoshimura and Tetsuro Sakumoto :
Impact of single embryo transfer policy on perinatal outcomes in fresh and frozen cycles-analysis of the Japanese Assisted Reproduction Technology registry between 2007 and 2012.,
Fertility and Sterility, Vol.105, No.2, 337-46.e3, 2015.

(要約): To investigate whether the introduction of single embryo transfer (SET) policy in Japan has improved perinatal outcomes. A retrospective cohort study. Not applicable. A total of 140,718 live births and 510 stillbirths (after 22 weeks of gestation) conceived by assisted reproductive technology in Japan between 2007 and 2012 were reviewed. None. Preterm birth (PTB), low birth weight (LBW), very low birth weight (VLBW), small for gestational age (SGA), large for gestational age (LGA), perinatal mortality, and other pregnancy complications. The rate of SET increased significantly from 52.2% in 2007 to 82.6% in 2012, while the rate of multiple pregnancy decreased significantly from 10.7% to 4.1% over the same period. The rates of PTB, LBW, and SGA decreased significantly, while that of LGA increased. Perinatal mortality decreased from 0.70% to 0.40% in fresh cycles, while that of frozen cycles did not change. Double ET or more was associated with a significantly increased risk for multiple pregnancy, placenta accreta, preterm premature rupture of membrane, cesarean section (CS), PTB, LBW, SGA, and early neonatal death compared with SET. Compared with before the SET policy was launched, the risks of multiple pregnancy, CS, early PTB before 32 weeks, LBW, VLBW, and SGA were significantly decreased after the policy was launched, with significant interactions of fresh/frozen status. The results suggest that the SET policy improved perinatal outcomes in Japan. The impact of SET policy was different in fresh and frozen cycles for several perinatal outcomes.
(キーワード): Adult / Cryopreservation / Embryo Culture Techniques / Female / Fertility / Fertilization in Vitro / Humans / Infant, Newborn / Infertility / Japan / Live Birth / Male / Perinatal Mortality / Policy Making / Pregnancy / Pregnancy Complications / Pregnancy Rate / Program Evaluation / Registries / Retrospective Studies / Risk Assessment / Risk Factors / Single Embryo Transfer / Time Factors / Treatment Outcome
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.fertnstert.2015.10.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26518122; ● Search Scopus @ Elsevier (PMID): 26518122; ● Search Scopus @ Elsevier (DOI): 10.1016/j.fertnstert.2015.10.002

(DOI: 10.1016/j.fertnstert.2015.10.002, PubMed: 26518122) Takeshi Katou, Kaoru Keyama, Sumika Matsui, Mikio Yamasaki, Kanako Yoshida and Minoru Irahara :
Step Up from Salpingo-Oophorectomy to Total Laparoscopic Hysterectomy Incorporating Incision Dissectiong Procedures.,
Journal of Minimally Invasive Gynecology, Vol.22, No.6S, S217, 2015.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jmig.2015.08.772
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27679084; ● Search Scopus @ Elsevier (PMID): 27679084; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jmig.2015.08.772

(DOI: 10.1016/j.jmig.2015.08.772, PubMed: 27679084) Anna Tani, S Yamamoto, Masahiko Maegawa, Koutaro Kunimi, Sumika Matsui, Kaoru Keyama, Takeshi Katou, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki, Toshiyuki Yasui, M Kamada, Takeshi Soeki, Masataka Sata and Minoru Irahara :
Arterial stiffness is increased in young women with endometriosis.,
Journal of Obstetrics and Gynaecology, Vol.35, No.7, 711-715, 2015.

(要約): Endometriosis is a chronic gynaecological disorder that is accompanied by inflammation and oxidative stress. Atherosclerosis has a long subclinical progression in arteries of children and young adults decades before overt clinical manifestations of the disease. In this study, we determined arterial stiffness by measuring brachial-ankle pulse wave velocity (baPWV) in women with endometriosis to assess the presence of subclinical atherosclerosis. We also measured markers of inflammation and oxidative stress in women with endometriosis. baPWV in women with endometriosis aged over 30 years was significantly higher than that in women without endometriosis aged over 30 years (p < 0.05), but not in women aged less than 30. Serum high-sensitivity C-reactive protein level in women with endometriosis was significantly higher than that in controls (p < 0.05). Young women with endometriosis show significantly increased arterial stiffness, suggesting that women with endometriosis need to be cautious of the future onset of atherosclerosis.
(キーワード): arterial stiffness / endometriosis / hs CRP / subclinical inflammation / young women
(出版サイトへのリンク): ● Publication site (DOI): 10.3109/01443615.2014.992871
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25543526; ● Summary page in Scopus @ Elsevier: 2-s2.0-84944158077

(DOI: 10.3109/01443615.2014.992871, PubMed: 25543526, Elsevier: Scopus) 安原由子, 飯藤大和, 桑村由美, 梅田弥生, 浦西由美, 谷岡哲也, 近藤和也, 岸田佐智, 苛原稔 :
徳島県内の看護職員高度人材育成研修推進事業における特定行為に係る実態調査,
四国医学雑誌, Vol.71, No.3,4, 59-70, 2015年.

(要約): The purpose of this survey was to determine nurse administrators and hospital directors' perspectives about nurses' abilities in performing specific medical practices in hospitals in Tokushima Prefecture. The nurse administrators and hospital directors of all hospitals (113 hospitals) in Tokushima Prefecture were invited to respond to a survey questionnaire by mail posted during the period between February and March of 2015. Responses were received from 46 nurse administrators (response rate, 41%) and 38 hospital directors (response rate, 34%). The obtained data were analyzed using descriptive statistics and the narrative descriptions summarized. Majority of responses from nurse administrators and hospital directors were accepting that nurses have responsibilities for administering medicines such as tranquilizers, anti-anxiety and anticonvulsant drugs. This result was not affected by the size of the hospital, in which fifty four percent (54%) of respondents were from hospitals with less than 100 beds. In Japan, only hospitals with 100 beds or more may have procedures involving highly invasive treatments. Because of this, many of the respondents answered ``not applicable'' to statements in the questionnaire which were related to invasive treatments. Recruitment of nurses, strategies to cope with accidents and education curriculum were important concerns of nurse administrators and hospital directors which remain to be answered in future investigations.
(キーワード): nurses' abilities in performing specific medical practices / Tokushima Prefecture / nurse administrators / hospital directors
(徳島大学機関リポジトリ): ● Metadata: 109920
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050282812441741824

(徳島大学機関リポジトリ: 109920, CiNii: 1050282812441741824) Yasuyo Saijo, Hiroyuki Furumoto, Kanako Yoshida, Masato Nishimura and Minoru Irahara :
Clinical Significance of Vascular Endothelial Growth Factor Expression and Microvessel Density in Invasive Cervical Cancer.,
The Journal of Medical Investigation : JMI, Vol.62, No.3-4, 154-160, 2015.

(要約): To determine whether vascular endothelial growth factor (VEGF) expression and microvessel density are predictive of prognosis in cases of invasive cervical cancer, correlations among VEGF expression, microvessel density, and clinicopathological parameters were identified. VEGF expression was evaluated in 50 cervical cancer samples by immunohistochemical staining. Microvessel density was assessed by immunostaining for CD31-positive endothelial cells in the most vascularized areas of tumors. VEGF expression and microvessel density were significantly higher in adenocarcinomas than in squamous cell carcinomas. However, in cases of adenocarcinoma, no significant correlations were found among VEGF expression, microvessel density, and clinicopathological parameters. In contrast, for squamous cell carcinomas, microvessel density was significantly higher in cases at an advanced stage and in those with several other poor prognostic factors. The finding that cervical adenocarcinomas exhibited greater VEGF expression and microvessel density than squamous cell carcinomas may explain the poorer prognosis of adenocarcinoma compared with squamous cell carcinoma. Moreover, microvessel density in squamous cell carcinomas was significantly correlated with poor prognostic factors. Therefore, there is possibility that bevacizumab, a humanized monoclonal antibody against VEGF-A, may be useful in the initial treatment targeting angiogenesis for early-stage cervical cancer.
(徳島大学機関リポジトリ): ● Metadata: 111271
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.62.154
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26399340; ● Search Scopus @ Elsevier (PMID): 26399340; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.62.154

(徳島大学機関リポジトリ: 111271, DOI: 10.2152/jmi.62.154, PubMed: 26399340) Kazuhisa Maeda, Issei Imoto, Takashi Kaji, Yukiko Yoshida, Souichiro Nakayama and Minoru Irahara :
Case of non-mosaic trisomy 20 in amniotic fluid cultures without anomalies in the fetus: Cytogenetic discrepancy between amniocytes and fetal blood.,
The Journal of Obstetrics and Gynaecology Research, Vol.41, No.1, 141-144, 2014.

(要約): We report a case of non-mosaic trisomy 20 detected prenatally by amniocentesis during the 16th week of pregnancy. Fetal blood sampling showed a normal karyotype and no fetal, neonatal or infant abnormalities were observed. Amniotic fluid cell karyotyping revealed a trisomy 20 (47,XY,+20) with 100% trisomic cells (38/38); however, a subsequent cordocentesis revealed a normal male karyotype. Moreover, a follow-up ultrasonographic examination did not reveal any major congenital malformations, and a healthy male infant was delivered subsequently at an appropriate gestational age without obvious anomalies. Cytogenetic analysis of blood lymphocytes from the infant revealed a normal karyotype, but cultured cells from the term placenta showed a mosaic karyotype 47,XY,+20/46,XY with 88% trisomic cells (44 of 50). Furthermore, no anomalies or developmental delays were observed in the neonatal period, thus suggesting two possibilities: confined placental mosaicism with the presence of normal and abnormal cell lineages, or generalized mosaicism affecting a limited number of tissues in both the placenta and fetus.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jog.12488
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25164874; ● Summary page in Scopus @ Elsevier: 2-s2.0-84920768226

(DOI: 10.1111/jog.12488, PubMed: 25164874, Elsevier: Scopus) Takeshi Iwasa, Toshiya Matsuzaki, Sumika Matsui, Munkhsaikhan Munkhzaya, Altankhuu Tungalagsuvd, Takako Kawami, Masahiro Murakami, Takeshi Katou, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
The effects of LPS-induced endotoxemia on the expression of adiponectin and its receptors in female rats.,
Endocrine Journal, Vol.61, No.9, 891-900, 2014.

(要約): Adiponectin (APN), secreted by white adipose tissue (WAT), acts as a protective factor against inflammatory conditions. However, the changes in the expression levels of endogenous APN and the two types of APN receptor (AdipoR1 and AdipoR2) induced by acute inflammatory conditions have not been fully elucidated. In this study, the changes in peripheral and/or central APN and AdipoR expression caused by lipopolysaccharide (LPS)-induced sepsis were examined in gonadal-intact (Sham) and ovariectomized (OVX) female rats. As it has been reported that APN and AdipoR suppress the production of inflammatory cytokines to prevent excessive inflammation, the mRNAs of these molecules were also examined. LPS injection induced increases in visceral WAT APN mRNA without affecting the serum APN level in both the Sham and OVX rats. OVX rats exhibited higher serum APN levels than Sham rats. LPS injection increased the subcutaneous WAT APN mRNA in OVX rats. In both Sham and OVX rats, LPS injection led to a decrease in hepatic AdipoR2 mRNA and an increase in hypothalamic AdipoR2 mRNA. Hypothalamic AdipoR2 mRNA was upregulated 24 h after LPS injection in OVX but not Sham rats. Serum TNF- level at 6 h after LPS injection and hypothalamic and hepatic IL-6 and TNF- mRNA at 24 h after LPS injection were significantly higher in Sham than OVX rats. These results suggest that APN and AdipoR play roles in modulating inflammation under septic conditions in female rats.
(キーワード): Adiponectin / Adipose Tissue, White / Animals / Endotoxemia / Female / Lipopolysaccharides / Liver / Male / Ovariectomy / RNA, Messenger / Rats, Sprague-Dawley / Receptors, Adiponectin
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ14-0042
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24974879; ● Summary page in Scopus @ Elsevier: 2-s2.0-84907507161

(DOI: 10.1507/endocrj.EJ14-0042, PubMed: 24974879, Elsevier: Scopus) Takeshi Iwasa, Toshiya Matsuzaki, Altankhuu Tungalagsuvd, Munkhsaikhan Munkhzaya, Takako Kawami, Hirobumi Niki, Takeshi Kato, Akira Kuwahara, Hirokazu Uemura, Toshiyuki Yasui and Minoru Irahara :
Hypothalamic Kiss1 and RFRP gene expressions are changed by a high dose of lipopolysaccharide in female rats.,
Hormones and Behavior, Vol.66, No.2, 309-316, 2014.

(要約): Reproductive function is suppressed by several types of stress. Hypothalamic kisspeptin, which is a product of the Kiss1 gene, and GnIH/RFRP have pivotal roles in the regulation of GnRH and gonadotropins through their receptors Kiss1r and GPR147 in many species. However, alterations of these factors under stress conditions have not been fully evaluated. This study investigated the mechanisms of immune stress-induced reproductive dysfunction, especially focusing on the changes of Kiss1 and RFRP gene expression. Serum LH levels and hypothalamic Kiss1 and GnRH mRNA levels were decreased, while hypothalamic RFRP and GPR147 mRNA levels were increased by administration of a high dose of LPS (5mg/kg) in both ovariectomized and gonadal intact female rats. In this condition, Kiss1 and/or RFRP mRNA levels were positively and negatively correlated with GnRH expression, respectively. In contrast, hypothalamic Kiss1, RFRP, and GPR147 mRNA levels were not changed by administration of a moderate dose of LPS (500g/kg) in ovariectomized rats. Rats with high-dose LPS injection showed more prolonged fever responses and severe anorexia compared with rats with moderate-dose LPS injection, indicating that more energy was used for the immune response in the former. These results suggest that the underlying mechanisms of dysfunction of gonadotropin secretion are changed according to the severity of immune stress, and that changes of some reserved factors, such as kisspeptin and RFRP, begin to participate in the suppression of GnRH and gonadotropin in severe conditions. As reproduction needs a large amount of energy, dysfunction of gonadotropin secretion under immune stress may be a biophylatic mechanism by which more energy is saved for the immune response.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.yhbeh.2014.06.007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24952104; ● Search Scopus @ Elsevier (PMID): 24952104; ● Search Scopus @ Elsevier (DOI): 10.1016/j.yhbeh.2014.06.007

(DOI: 10.1016/j.yhbeh.2014.06.007, PubMed: 24952104) Sumika Matsui, Toshiyuki Yasui, Kaoru Keyama, Anna Tani, Takeshi Katou, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
High adiponectin level in late postmenopausal women with normal renal function.,
Clinica Chimica Acta, Vol.430, 104-108, 2014.

(要約): We examined whether high circulating adiponectin level is associated with renal function and is favorable for lipid and glucose metabolism in late postmenopausal women with normal renal function. We conducted a cross-sectional study in 115 postmenopausal women and divided the subjects into 2 groups (early postmenopausal women and late postmenopausal women). Serum levels of adiponectin, blood urea nitrogen, creatinine (Cr), glucose, insulin and lipid profiles were measured. Glomerular filtration rate (GFR) was estimated by age and Cr. Serum adiponectin level in late postmenopausal women was significantly higher than that in early postmenopausal women, and eGFR in late postmenopausal women was significantly lower than that in early postmenopausal women. Adiponectin level showed a negative correlation with eGFR and tended to have a negative correlation with eGFR after adjustments for age, BMI and bioavailable testosterone in all subjects, but adiponectin level did not show a significant correlation with eGFR in late postmenopausal women. Adiponectin level in late postmenopausal women showed a significant negative correlation with triglyceride (TG) and a positive correlation with high-density lipoprotein cholesterol (HDL-C) after adjustments for age and BMI. In late postmenopausal women with normal renal function, high adiponectin level is associated with favorable lipid profiles. High adiponectin level may be involved in not only eGFR but also other factors in late postmenopausal women.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cca.2013.12.037
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24389055; ● Summary page in Scopus @ Elsevier: 2-s2.0-84893117904

(DOI: 10.1016/j.cca.2013.12.037, PubMed: 24389055, Elsevier: Scopus) Katsuyoshi Kume, Yasuo Tsutsumi M, Tomohiro Soga, Yoko Sakai, Noriko Kambe, Ryosuke Kawanishi, Eisuke Hamaguchi, Tomiya Kawahara, Asuka Kasai, Yoshimi Nakaji, Yousuke Horikawa T, Souichiro Nakayama, Takashi Kaji, Minoru Irahara and Katsuya Tanaka :
A case of placenta percreta with massive hemorrhage during cesarean section.,
The Journal of Medical Investigation : JMI, Vol.61, No.1-2, 208-212, 2014.

(要約): We describe a case of a 39-year-old woman diagnosed with placenta percreta complicated by massive hemorrhage during a cesarean section. At 27 weeks of gestation, she underwent an emergency cesarean section under general anesthesia for vaginal bleeding and an intrauterine infection. Soon after delivery, a massive hemorrhage was encountered while attempting to separate the placenta percreta from the bladder wall. Although total abdominal hysterectomy and partial cystectomy were performed, massive hemorrhaging persisted. Bleeding was finally controlled following bilateral internal iliac artery embolization. We used a cell salvage device and a rapid infuser for hemodynamics stabilization. Total blood loss was 47,000 mL, and anesthesia time was 12 h and 47 min. The patient was discharged on the 32(nd) postoperative day without major complications. Placenta accreta can be associated with life-threatening hemorrhage and it is vital to plan accordingly preoperatively.
(キーワード): Adult / Blood Loss, Surgical / Cesarean Section / Cystectomy / Embolization, Therapeutic / Female / Hemodynamics / Hemorrhage / Humans / Hysterectomy / Placenta Accreta / Pregnancy / Treatment Outcome
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.61.208
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24705768; ● Search Scopus @ Elsevier (PMID): 24705768; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.61.208

(DOI: 10.2152/jmi.61.208, PubMed: 24705768) Hirobumi Niki, Toshiya Matsuzaki, Riyo Kinouchi, Takeshi Iwasa, Takako Kawami, Takeshi Katou, Akira Kuwahara and Minoru Irahara :
Improvement in diagnostic performance of the revised total testosterone measuring system in Japanese women with polycystic ovary syndrome.,
The Journal of Medical Investigation : JMI, Vol.61, No.1-2, 65-71, 2014.

(要約): Hyperandrogenemia has become an important factor in the diagnosis of polycystic ovary syndrome (PCOS), in Japan as well as Western countries, since the Japanese PCOS diagnostic criteria were revised in 2007. The revised electrochemiluminescence immunoassay (ECLIA) reagent for total testosterone (T) (ECLusys TESTO II; New T assay), which has lower cross-reactivity with DHEA-S than the previous reagent (ECLusys TESTO I; Old T assay), has recently been developed. We studied the improvement between New and Old T assays in regards to the diagnostic performance of serum T for patients with PCOS. Serum T levels were measured in both normal women (Control, n=92) and PCOS patients (n=86). The rate of elevated T using each kit was significantly higher in the PCOS group than in the Control group, and significantly higher using New T assay (38/86, 44.2%) compared with Old T assay (26/86, 30.2%). A significant correlation was found between T level and body mass index, but not LH level. Furthermore, higher detection rates of hormonal abnormalities were seen in PCOS patients using combined measurement of both T and LH (63/80) compared with single measurement of either T (37/80) or LH (36/80). Therefore, the serum T assay is essential for the diagnosis of PCOS, and New T assay combined with the LH assay may improve the diagnostic process for PCOS. J. Med. Invest. 61: 65-71, February, 2014.
(キーワード): Adult / Asian Continental Ancestry Group / Biological Markers / Body Mass Index / Case-Control Studies / Diagnostic Tests, Routine / Electrochemical Techniques / Female / Humans / 日本 (Japan) / Luminescent Measurements / Outcome Assessment (Health Care) / Polycystic Ovary Syndrome / Sensitivity and Specificity / Testosterone
(徳島大学機関リポジトリ): ● Metadata: 109522
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.61.65
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24705751; ● Summary page in Scopus @ Elsevier: 2-s2.0-84897944473

(徳島大学機関リポジトリ: 109522, DOI: 10.2152/jmi.61.65, PubMed: 24705751, Elsevier: Scopus) Sumika Matsui, Toshiyuki Yasui, A Tani, Takeshi Katou, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki, Kokichi Arisawa and Minoru Irahara :
Effect of ultra-low-dose estradiol and dydrogesterone on arterial stiffness in postmenopausal women.,
Climacteric, Vol.17, No.2, 191-196, 2013.

(要約): Abstract Background Ultra-low-dose estradiol is known to improve menopausal symptoms and increase bone mineral density. However, the effect of ultra-low-dose estradiol on vascular function has not been clarified. Objectives We examined the effects of ultra-low-dose estradiol on brachial-ankle pulse wave velocity (baPWV) and circulating markers of cardiovascular risk. Patients and methods Twenty-eight postmenopausal women were enrolled in this study. Fourteen women received oral estradiol (0.5 mg) and dydrogesterone (5 mg) every day for 12 months (ultra-low-dose group) as hormone replacement therapy (HRT) and 14 women as a control group did not receive HRT. The baPWV, lipid profiles, homeostasis model assessment of insulin resistance (HOMA-IR) and vascular inflammatory markers were measured. Results The baPWV level significantly decreased in the ultra-low-dose group (p = 0.037), while the baPWV level did not significantly change in the control group. HOMA-IR tended to decrease in the ultra-low-dose group (p = 0.076). Systolic blood pressure and diastolic blood pressure did not change significantly in either group. Conclusion An HRT regimen using oral ultra-low-dose estradiol and dydrogesterone has an effect on arterial stiffness and insulin resistance.
(出版サイトへのリンク): ● Publication site (DOI): 10.3109/13697137.2013.856399
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24164272; ● Search Scopus @ Elsevier (PMID): 24164272; ● Search Scopus @ Elsevier (DOI): 10.3109/13697137.2013.856399

(DOI: 10.3109/13697137.2013.856399, PubMed: 24164272) Sumika Matsui, Toshiyuki Yasui, Anna Tani, Takeshi Katou, Kotaro Kunimi, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Difference in the Ratio of High-Molecular Weight (HMW) to Total Adiponectin and HMW Adiponectin in Late Postmenopausal Women.,
Journal of Endocrinological Investigation, 2013.

(要約): Objective: High-molecular weight (HMW) isoform level and HMW ratio have been shown to be better predictors of insulin sensitivity and metabolic syndrome than total adiponectin level. We examined the changes in circulating levels of HMW adiponectin and ratios of HMW to total adiponectin in women during the menopausal transition. Methods: We conducted a cross-sectional study in 217 healthy women and divided them into 4 stages: 58 women in premenopausal, 69 women in perimenpausal, 62 women in early postmenopausal and 28 women in late postmenopausal women. Serum levels of total adiponectin and HMW adiponectin were measured by an enzyme-linked immunosorbent assay. Results: In late postmenopausal women, HMW adiponectin level was significantly higher than that in perimenopausal women and the HMW to total adiponectin ratio was significantly lower than that in early postmenopausal women. In perimenopausal women, HMW adiponectin level was significantly lower than that in premenopausal women and HMW to total adiponectin ratio was significantly lower than the ratios in premenopausal and early postmenopausal women. Conclusion: The ratio of HMW to total adiponectin is low in late postmenopausal women, though both levels of total and HMW adiponectin were high after menopause in our cross-sectional study.
(出版サイトへのリンク): ● Publication site (DOI): 10.3275/9001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23765418; ● Search Scopus @ Elsevier (PMID): 23765418; ● Search Scopus @ Elsevier (DOI): 10.3275/9001

(DOI: 10.3275/9001, PubMed: 23765418) Sumika Matsui, Toshiyuki Yasui, Anna Tani, Kotaro Kunimi, Hirokazu Uemura, Satoshi Yamamoto, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Associations of estrogen and testosterone with insulin resistance in pre- and postmenopausal women with and without hormone therapy.,
International Journal of Endocrinology and Metabolism, Vol.11, No.2, 65-70, 2013.

(要約): Estrogen deficiency due to natural menopause or surgical menopause has been suggested to have an adverse effect on insulin resistance. Testosterone and sex hormone-binding globulin (SHBG) as well as estrogen are also associated with insulin resistance in women. However, to date, the associations of estradiol, testosterone and SHBG with insulin resistance according to estrogen level have not been clarified. We examined the associations of estradiol, testosterone and SHBG with insulin resistance in pre- and in postmenopausal women and postmenopausal women who had received hormone therapy to clarify whether the associations differ depending on the estrogen status. Twenty premenopausal women and thirty-two postmenopausal women were enrolled in this study. Fifteen postmenopausal women received oral conjugated equine estrogen (CEE) (0.625 mg) everyday for 12 months. Serum levels of estradiol, testosterone, SHBG and insulin and plasma levels of glucose were measured. Serum estradiol levels tended to have a negative correlation with homeostasis model assessment of insulin resistance (HOMA-IR) in premenopausal women but not in postmenopausal women. On the other hand, free testosterone levels tended to have a positive correlation with HOMA-IR in postmenopausal women but not in premenopausal women. Serum SHBG levels showed significant negative correlations with HOMA-IR in both pre- and postmenopausal women. SHBG level was significantly increased, free testosterone level was significantly decreased and HOMA-IR was significantly decreased at 12 months after CEE administration. However, there were no significant correlations of changes between estradiol, SHBG or free testosterone and HOMA-IR. The associations of sex steroid hormones with insulin resistance are different depending on the estrogen status.
(出版サイトへのリンク): ● Publication site (DOI): 10.5812/ijem.5333
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23825975; ● Summary page in Scopus @ Elsevier: 2-s2.0-84876848958

(DOI: 10.5812/ijem.5333, PubMed: 23825975, Elsevier: Scopus) 河見貴子, 古本博孝, 吉田加奈子, 加藤剛志, 西村正人, 苛原稔 :
子宮頸癌放射線療法後の局所再発に対する子宮摘出の有用性と安全性について,
現代産婦人科, Vol.61, No.2, 321-324, 2013年.

(キーワード): 再発子宮頸癌 / concurrent chemoradiation (CCRT) / 子宮全摘
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520009409864859776

(CiNii: 1520009409864859776) Takeshi Iwasa, Toshiya Matsuzaki, Riyo Kinouchi, Ganbat Gereltsetseg, Masahiro Murakami, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Developmental changes in the responsiveness of hypothalamic ER alpha mRNA levels to food deprivation.,
Neuroendocrinology Letters, Vol.34, No.6, 543-548, 2013.

(要約): Estrogen plays an important role in feeding and energy balance, and the critical role of estrogen in the control of appetite and energy balance is mediated by hypothalamic estrogen receptor (ER) alpha. In undernourished rodents, hypothalamic ER alpha mRNA expression are decreased. Responses of some hypothalamic factors to negative energy balance develop during the early neonatal period. In this study, we examined the developmental changes of fasting-induced alterations in hypothalamic ER alpha mRNA expression in female rats. ER alpha mRNA expression was reduced after a 12-h or 24-h fast at postnatal days 15 and 25, but not at day 5. Serum estradiol levels in postnatal day 25 rats were not changed by fasting. Although serum leptin levels were suppressed by fasting at all ages, hypothalamic ER alpha mRNA expression at postnatal day 25 was not changed by leptin administration after a 24-h fast. These data show that the sensitivity of hypothalamic ER alpha to negative energy balance may not be established in the early neonatal period, and that it develops by postnatal day 15. Decreased leptin levels might not be involved in the alterations of hypothalamic ER alpha mRNA expression in the undernourished condition.
(キーワード): Age Factors / Animals / Animals, Newborn / Body Weight / Energy Metabolism / Estrogen Receptor alpha / Female / Food Deprivation / Hypothalamus / Leptin / Pregnancy / RNA, Messenger / Rats
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24378445; ● Search Scopus @ Elsevier (PMID): 24378445

(PubMed: 24378445) Satoru Yoshie, Masanori Nishinaga, Shohei Kawagoe, Satoshi Hirahara, Shinsuke Fujita, 苛原稔, Yoriko Anzai, Shigeru Onozawa, Yoshiya Oishi, Hiroshi Suzuki, Miyuki Numata, Fumie Katayama, Hiroshi Murayama, Rumiko Tsuchiya, Mari Kimata, Kouji Shibasaki, Katsuya Iijima, Tetsuo Tsuji :
[Development of a home care educational program for community physicians and other professionals-a trial in Kashiwa City].,
癌と化学療法, Vol.39 Suppl 1, 80-85, 2012年.

(要約): From May to October 2011, we conducted an 8-day homecare educational program for physicians, dentists, pharmacists, visiting nurses, long-term care managers, and hospital staff in Kashiwa city, Chiba, which was primarily intended to increase home visits by physicians. The characteristics of the program were as follows: 1) active and busy community physician participation, 2) attendance of practical training by physicians, 3) interprofessional discussion, 4) recruitment of participants from the same city, 5) recommendation of participant recruitment by a community-level professional association such as Kashiwa City Medical Association. By comparison of the pre- and post-program questionnaires completed by participants, the motivation for homecare practice, knowledge about homecare, and interactions with other professionals have increased. We will further standardize and generalize this program in order to contribute to homecare promotion in Japan.
(キーワード): Community Networks / Education, Medical, Continuing / Home Care Services / Japan
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23268908; ● Search Scopus @ Elsevier (PMID): 23268908

(PubMed: 23268908) Riyo Kinouchi, Toshiya Matsuzaki, Takeshi Iwasa, Ganbat Gereltsetseg, Hiroshi Nakazawa, Kotaro Kunimi, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Prepubertal exposure to glucocorticoid delays puberty independent of the hypothalamic Kiss1-GnRH system in female rats.,
International Journal of Developmental Neuroscience, Vol.30, No.7, 596-601, 2012.

(要約): Secretion of glucocorticoids is widely known as a key endocrine response to stresses. Prenatal dexamethasone administration induces intrauterine growth retardation and delayed onset of puberty in female rats independent of the hypothalamic Kiss1-gonadotropin-releasing hormone (GnRH) system. The aim of this study was to evaluate the influence of chronic intracerebroventricular (central, CD) or subcutaneous (peripheral, PD) dexamethasone administration to prepubertal female rats on the onset of puberty and body weight change. Rats administered dexamethasone from day 25 to day 34 (CD and PD) showed significantly reduced body weight gain throughout the experimental period and delayed onset of vaginal opening compared with rats administered saline centrally (CS) or peripherally (PS). At 34 days old, hypothalamic Kiss1r mRNA levels were significantly lower with CD than with CS. No significant differences were seen between rats administered saline and rats administered dexamethasone with regard to hypothalamic Kiss1, GnRH and NPY mRNA levels or serum LH levels. Serum leptin concentrations were higher in CD and PD than in the controls (CS and PS). These results suggest that the delayed onset of puberty induced by prepubertal dexamethasone administration occurs independent of the hypothalamic Kiss1-GnRH system.
(キーワード): Animals / Body Weight / Brain / Female / Glucocorticoids / Gonadotropin-Releasing Hormone / Growth / Hormones / Hypothalamus / Infusion Pumps / Kisspeptins / Organ Size / RNA, Messenger / Rats / Rats, Sprague-Dawley / Real-Time Polymerase Chain Reaction / Sexual Maturation / Vagina
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2012.09.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22982503; ● Search Scopus @ Elsevier (PMID): 22982503; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2012.09.001

(DOI: 10.1016/j.ijdevneu.2012.09.001, PubMed: 22982503) Sumika Matsui, Toshiyuki Yasui, Anna Tani, Takeshi Katou, Kotaro Kunimi, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Association of circulating adiponectin with testosterone in women during the menopausal transition.,
Maturitas, Vol.73, No.3, 255-260, 2012.

(要約): We examined (1) the change in circulating adiponectin in women during the menopausal transition and (2) the associations of adiponectin levels with estrogen, androgen and sex hormone-binding globulin (SHBG) in women during the menopausal transition. We conducted a cross-sectional study in 235 healthy women and divided them into 7 stages by menstrual regularity and follicle-stimulating hormone (FSH) level. Serum levels of adiponectin, estradiol, total testosterone, dehydroepiandrosterone-sulfate (DHEA-S) and SHBG were measured. Levels of free and bioavailable testosterone were calculated by using total testosterone, albumin and SHBG. Serum adiponectin levels showed a U-curve, levels being low in early and late menopausal transition and gradually becoming higher after menopause. Adiponectin levels were negatively correlated with levels of free testosterone, bioavailable testosterone and DHEA-S and were positively correlated with SHBG in postmenopausal women for whom more than 1 year had passed since menopause. Adiponectin level was not correlated with estradiol level. Circulating adiponectin level shows a U-curve during the menopausal transition and adiponectin level is associated with levels of free and bioavailable testosterone and DHEA-S in postmenopause.
(キーワード): Adiponectin / Adult / Biological Availability / Cross-Sectional Studies / Dehydroepiandrosterone Sulfate / Estradiol / Estrogens / Female / Follicle Stimulating Hormone / Humans / Menopause / Middle Aged / Sex Hormone-Binding Globulin / Testosterone
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.maturitas.2012.08.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22939588; ● Search Scopus @ Elsevier (PMID): 22939588; ● Search Scopus @ Elsevier (DOI): 10.1016/j.maturitas.2012.08.003

(DOI: 10.1016/j.maturitas.2012.08.003, PubMed: 22939588) 松井寿美佳, 安井敏之, 山本哲史, 上村浩一, 苛原稔 :
更年期障害 (特集ホルモン補充療法の最新知識 : リスク・ベネフィットを今,どう考えるか),
産科と婦人科, Vol.79, No.4, 413-419, 2012年.

(キーワード): エストロゲン / 種類 / 投与ルート / 更年期障害
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521980704854898944

(CiNii: 1521980704854898944) Takashi Kaji, K Maeda, M Suto, M Sato and Minoru Irahara :
Simultaneous recordings of pulsed wave Doppler signals in hepatic vein and descending aorta using dual Doppler: a novel method for evaluating fetal arrhythmias.,
Ultrasound in Obstetrics & Gynecology, Vol.39, No.3, 357-359, 2012.

(キーワード): Aorta, Thoracic / Arrhythmias, Cardiac / Blood Flow Velocity / Female / Gestational Age / Hepatic Veins / Humans / Pregnancy / Ultrasonography, Doppler, Pulsed / Ultrasonography, Prenatal
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/uog.10096
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21898640; ● Search Scopus @ Elsevier (PMID): 21898640; ● Search Scopus @ Elsevier (DOI): 10.1002/uog.10096

(DOI: 10.1002/uog.10096, PubMed: 21898640) Sumika Matsui, Toshiyuki Yasui, Anna Tani, Kotaro Kunimi, Hirokazu Uemura, Satoshi Yamamoto, Akira Kuwahara, Toshiya Matsuzaki, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Changes in insulin sensitivity during GnRH agonist treatment in premenopausal women with leiomyoma.,
Clinica Chimica Acta, Vol.413, No.11-12, 960-965, 2012.

(要約): The purpose of this study was to determine (1) the influence of estrogen deficiency induced by gonadotropin-releasing hormone (GnRH) agonist administration on insulin sensitivity as well as hormones and factors related to insulin resistance and (2) the differences in the influence for these parameters by the degree of basal insulin sensitivity. Thirty-five women diagnosed with leiomyoma were enrolled in this study. Serum levels of fasting glucose, insulin, sex steroid hormones, sex hormone-binding globulin (SHBG), vascular inflammatory markers and cytokines before and at 6months after commencement of GnRH agonist administration were examined. In all women, levels of insulin, glucose and homeostasis model assessment of insulin resistance (HOMA-IR) were not significantly changed. However, in women who had a low HOMA-IR before treatment, levels of insulin, glucose and HOMA-IR showed significant increases and total testosterone level showed a significant decrease. In women who had a high HOMA-IR, levels of insulin, HOMA-IR and SHBG were significantly decreased and levels of highly sensitive C-reactive protein, soluble intercellular adhesion molecule-1, E-selectin and monocyte chemoattractant protein-1 were significantly increased. Change in insulin sensitivity caused by GnRH agonist administration for premenopausal women with leiomyoma differs depending on baseline insulin sensitivity before treatment.
(キーワード): Adult / Antineoplastic Agents, Hormonal / Blood Glucose / Body Mass Index / C-Reactive Protein / Chemokine CCL2 / 細胞質分裂 (cytokinesis) / E-Selectin / Female / Gonadotropin-Releasing Hormone / Humans / インスリン (insulin) / インスリン抵抗性 (insulin resistance) / Intercellular Adhesion Molecule-1 / Leiomyoma / Leuprolide / Middle Aged / Premenopause / Sex Hormone-Binding Globulin / Testosterone / Uterine Neoplasms
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cca.2012.01.040
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22366120; ● Search Scopus @ Elsevier (PMID): 22366120; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cca.2012.01.040

(DOI: 10.1016/j.cca.2012.01.040, PubMed: 22366120) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Riyo Kinouchi, Tomohiro Osugi, Ganbat Gereltsetseg, Shinobu Yoshida, Minoru Irahara and Kazuyoshi Tsutsui :
Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus.,
International Journal of Developmental Neuroscience, Vol.30, No.1, 31-37, 2012.

(要約): The mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) is considered to act on gonadotropin-releasing hormone (GnRH) neurons and on the pituitary to inhibit gonadotropin release and synthesis. To understand the functional significance of this neuropeptide, we investigated the physiological changes in RFRP at mRNA and peptide levels, as well as at the mRNA level of its cognate receptor, G protein-coupled receptor 147 (GPR147), in the rat hypothalamus during development. We also investigated the effects of gonadal steroids on mRNA expression levels of these molecules. In male rats, mRNA expressions of both RFRP and GPR147 increased from postnatal days 12 and 16, peaking at postnatal days 35 and 42, respectively. However, their expressions fell at postnatal day 49. In female rats, mRNA expression of RFRP continued to increase throughout development; mRNA expression of GPR147 in female rats increased from postnatal day 16, peaking at postnatal day 28, but decreased from postnatal day 35. The hypothalamic contents of RFRP on postnatal days 28 and 42 were significantly higher than on postnatal day 4 in male rats, and those on postnatal day 42 were significantly higher than those on postnatal days 4 and 28 in females. Neither orchidectomy nor ovariectomy influenced mRNA expression levels of RFRP or GPR147 in the prepubertal period when endogenous sex steroid levels were low in males and females. Administration of estradiol-17β (E2) increased mRNA expression of RFRP in prepubertal females. These results suggest that the hypothalamic RFRP system changes during development. An ovarian sex steroid, E2, may stimulate mRNA expression of RFRP in the prepubertal period when the basal E2 concentration is low.
(キーワード): Age Factors / Aging / Animals / Animals, Newborn / Estradiol / Female / Gene Expression Regulation, Developmental / Hypothalamus / Male / Neuropeptides / RNA, Messenger / Rats / Rats, Sprague-Dawley / Receptors, Neuropeptide / Sex Characteristics / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2011.10.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22064075; ● Search Scopus @ Elsevier (PMID): 22064075; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2011.10.003

(DOI: 10.1016/j.ijdevneu.2011.10.003, PubMed: 22064075) Yoshio Kasahara, Masaaki Kawai, Ichiro Tsuji, Eriko Tohno, Takao Yokoe, Minoru Irahara, Akira Tangoku and Noriaki Ohuchi :
Harms of screening mammography for breast cancer in Japanese women.,
Breast Cancer, 2012.

(要約): BACKGROUND: The US Preventative Services Task Force assesses the efficacy of breast cancer screening by the sum of its benefits and harms, and recommends against routine screening mammography because of its relatively great harms for women aged 40-49 years. Assessment of the efficacy of screening mammography should take into consideration not only its benefits but also its harms, but data regarding those harms are lacking for Japanese women. METHODS: In 2008 we collected screening mammography data from 144,848 participants from five Japanese prefectures by age bracket to assess the harms [false-positive results, performance of unnecessary additional imaging, fine-needle aspiration cytology (FNA), and biopsy and its procedures]. RESULTS: The rate of cancer detected in women aged 40-49 years was 0.28%. The false-positive rate (9.6%) and rates of additional imaging by mammography (5.8%) and ultrasound (7.3%) were higher in women aged 40-49 years than in the other age brackets. The rates of FNA (1.6%) and biopsy (0.7%) were also highest in women aged 40-49 years. However, they seemed to be lower than the rates reported by the Breast Cancer Surveillance Consortium (BCSC) and other studies in the US. CONCLUSIONS: The results, although preliminary, indicate the possibility that the harms of screening mammography for Japanese women are less than those for American women.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12282-012-0333-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22282164; ● Search Scopus @ Elsevier (PMID): 22282164; ● Search Scopus @ Elsevier (DOI): 10.1007/s12282-012-0333-6

(DOI: 10.1007/s12282-012-0333-6, PubMed: 22282164) 松井寿美佳, 安井敏之, 中山聡一朗, 佐藤美紀, 加地剛, 山本哲史, 上村浩一, 前田和寿, 苛原稔 :
単胎妊婦と双胎妊婦におけるカルシウム代謝ならびにビタミンDの動態の比較 (第22回婦人科骨粗鬆症研究会) -- (WORKSHOP 若年女性における骨の管理),
オステオポローシスジャパン : 日本骨粗鬆症学会雑誌, Vol.20, No.3, 517-519, 2012年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523388080504231296

(CiNii: 1523388080504231296) Tomomi Iba, Yuya Yano, Mayumi Umeno, Kenji Hinokio, Akira Kuwahara, Minoru Irahara, Shuji Yamano and Toshiyuki Yasui :
Roscovitine in combination with calcium ionophore induces oocyte activation through reduction of M-phase promoting factor activity in mice.,
Zygote, Vol.20, No.4, 321-325, 2011.

(要約): SummaryThe aim of the present study was to determine oocyte activation and change in M-phase promoting factor (MPF) activity induced by treatment with calcium ionophore and roscovitine in comparison with those induced by treatment with roscovitine alone and treatment with calcium ionophore and puromycin in mice. Freshly ovulated oocytes obtained from 6-8-week-old mice were divided into five groups (no activation treatment; 5 μM calcium ionophore A23187; 50 μM roscovitine; 5 μM calcium ionophore and 10 μg/ml puromycin; and 5 μM calcium ionophore and 50 μM roscovitine) and were incubated for 6 h. Oocyte activation, assessed by morphological changes, and changes in MPF activity in the five groups at 0, 2, 4 and 6 h of incubation were examined. Activated oocytes were defined as oocytes with at least one pronucleus. Oocytes treated with roscovitine alone were not activated during the 6-h incubation period. All of the oocytes in the calcium ionophore with puromycin group and in the calcium ionophore with roscovitine group were activated. The percentage activity of MPF in oocytes treated with roscovitine alone was decreased after 2 h and increased after 4 h of incubation. The percentage activity of MPF in oocytes treated with calcium ionophore and roscovitine was significantly decreased with suppression of MPF activity being maintained for 6 h, and this change was similar to that in oocytes treated with calcium ionophore and puromycin. Roscovitine with calcium ionophore is effective for induction of oocyte activation through suppression of MPF activity in mice.
(キーワード): Animals / Calcium Ionophores / Female / Maturation-Promoting Factor / Mice / Mice, Inbred Strains / Oocytes / Ovulation / Purines / Puromycin
(出版サイトへのリンク): ● Publication site (DOI): 10.1017/S0967199411000591
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22008472; ● Search Scopus @ Elsevier (PMID): 22008472; ● Search Scopus @ Elsevier (DOI): 10.1017/S0967199411000591

(DOI: 10.1017/S0967199411000591, PubMed: 22008472) Toshiya Matsuzaki, Takeshi Iwasa, Riyo Kinouchi, Shinobu Yoshida, Masahiro Murakami, Ganbat Gereltsetseg, Satoshi Yamamoto, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Fasting reduces the kiss1 mRNA levels in the caudal hypothalamus of gonadally intact adult female rats.,
Endocrine Journal, Vol.58, No.11, 1003-1012, 2011.

(要約): Kisspeptin, which is the product of the kiss1 gene and its receptor kiss1r, have emerged as the essential gatekeepers of reproduction. The present study used gonadally intact female rats to evaluate fasting-induced suppression of the KiSS-1 system of anteroventral periventricular nucleus (AVPV) and arcuate nucleus (ARC) under normal physiological conditions. Starting on the day of estrous, one group of rats was subjected to 72 h of food deprivation, while the other group of rats was able to continue feeding ad libitum. The length of the estrous cycle was significantly longer in the food-deprived rats as compared to the feeding rats. At the end of the 72-h food deprivation period, all of the food-deprived rats were at the diestrous phase, with their serum concentrations of LH and leptin significantly lower than that observed in the feeding rats. In addition, as compared to the feeding rats, the expression levels of kiss1 mRNA were significantly lower in the food-deprived rats in the posterior hypothalamic block, which contained the ARC, but not in the anterior hypothalamic block, which contain the AVPV. However, both the kiss1r mRNA expression levels in the anterior and posterior hypothalamic blocks and the neurokinin B and neurokinin 3 receptor mRNA expression levels in the posterior hypothalamic block were not significantly different between the feeding and food-deprived rats. Thus, lower kiss1 mRNA levels in the ARC appear to be responsible for the fasting-induced inhibition of gonadotrophin secretion and subsequent prolongation of the estrous cycle.
(キーワード): Animals / Estrous Cycle / Female / Food Deprivation / Hypothalamus / Kisspeptins / Leptin / Luteinizing Hormone / Neurokinin B / RNA, Messenger / Rats / Rats, Sprague-Dawley / Real-Time Polymerase Chain Reaction / Receptors, G-Protein-Coupled / Receptors, Neurokinin-3 / Reverse Transcriptase Polymerase Chain Reaction
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.K11E-131
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21979277; ● Search Scopus @ Elsevier (PMID): 21979277; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.K11E-131

(DOI: 10.1507/endocrj.K11E-131, PubMed: 21979277) 中澤浩志, 松崎利也, 岩佐武, 木内理世, 苛原稔 :
高プロラクチン血症として管理されていたマクロプロラクチン血症の2症例,
現代産婦人科, Vol.60, No.1, 179-184, 2011年.

(キーワード): マクロプロラクチン / プロラクチン / 高プロラクチン血症 / 月経不順 / macroprolactin / prolactin / hyperprolactinemia / irregular menses
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520853834794484608

(CiNii: 1520853834794484608) 東元あゆか, 加藤剛志, 阿部彰子, 海老沢桂子, 吉田加奈子, 西村正人, 古本博孝, 苛原稔 :
当院におけるプラチナ耐性再発卵巣癌に対するドキシルの使用経験,
現代産婦人科, Vol.60, No.1, 173-177, 2011年.

(キーワード): ドキシル / 再発 / 卵巣癌 / Doxil (PLD) / recurrent / ovarian cancer / salvage chemotherapy
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520009409869175424

(CiNii: 1520009409869175424) 冨士田祥子, 國見幸太郎, 海老沢桂子, 谷杏奈, 加藤剛志, 古本博孝, 苛原稔 :
骨盤内腫瘍の術後にたこつぼ心筋症を来した一症例,
現代産婦人科, Vol.60, No.1, 156-161, 2011年.

(キーワード): たこつぼ心筋症 / ストレス / カテコラミン / エストロゲン / Takotsubo cardiomyopathy / stress / estrogen
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520853834799832832

(CiNii: 1520853834799832832) Takeshi Iwasa, Toshiya Matsuzaki, Riyo Kinouchi, Ganbat Gereltsetseg, Masahiro Murakami, Hiroshi Nakazawa, Shinobu Fujisawa, Satoshi Yamamoto, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Effect of immune stress on body weight regulation is altered by ovariectomy in female rats.,
Journal of Reproductive Immunology, Vol.91, No.1-2, 41-47, 2011.

(要約): It has been suggested that obesity and loss of ovarian function alter the inflammatory response to immune stress. Ovariectomized (OVX) rats, which are used as a model of human menopause, exhibit both hyperphagia-induced obesity and gonadal steroid deficiency. To evaluate the effects of ovariectomy on inflammatory responses, we compared the anorectic response to LPS in OVX rats and gonad intact female rats. As leptin and hypothalamic interleukin-1β (IL1β) play pivotal roles in the anorectic response to immune stress, these factors were also measured. It was found that the OVX rats exhibited an increased anorectic response to LPS compared with the sham-operated rats. The OVX rats showed higher serum leptin concentrations and a greater increase in hypothalamic IL1β mRNA expression after LPS injection. In addition, in order to determine whether gonadal steroid deficiency contributes to the changes in the inflammatory responses of OVX rats, we compared responses between OVX rats treated with gonadal steroids and untreated OVX rats. There were no differences in appetite, the serum leptin level, and hypothalamic IL1β mRNA expression between the two groups after LPS injection. These findings suggest that the loss of ovarian function increases the induction of leptin and hypothalamic IL1β synthesis and consequently increases the anorectic response under immune stress conditions. It is possible that these alterations are caused by OVX-induced obesity rather than the direct effects of gonadal steroid deficiency.
(キーワード): Animals / Body Weight / Female / Gene Expression Regulation / Humans / Hypothalamus / Interleukin-1beta / Leptin / Lipopolysaccharides / Menopause / Models, Biological / Ovariectomy / Ovary / RNA, Messenger / Rats / Rats, Sprague-Dawley / Stress, Physiological
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jri.2011.05.010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21855150; ● Search Scopus @ Elsevier (PMID): 21855150; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jri.2011.05.010

(DOI: 10.1016/j.jri.2011.05.010, PubMed: 21855150) Kanako Yoshida, Hiroyuki Furumoto, Akiko Abe, Takeshi Katou, Masato Nishimura and Minoru Irahara :
The possibility of vertical transmission of human papillomavirus through maternal milk,
Journal of Obstetrics and Gynaecology, Vol.31, No.6, 503-506, 2011.

(要約): Human papillomavirus (HPV) DNA has been detected in the oral cavity of infants and breast cancer tissue, suggesting its vertical transmission through maternal milk. We determined whether HPV is detected in maternal milk and is vertically transmitted by breast-feeding. Informed consent was obtained, and maternal milk samples (n=80) were analysed for high-risk HPV DNA. In 43 women, this DNA was measured in the uterine cervix. In women with positive samples, this DNA was measured in the oral cavities of their children. The domain including HPV E6 and E7 was amplified by polymerase chain reaction using consensus primers, and HPV serotype determined by electrophoresis after restriction enzyme digestion. High-risk HPV-16 was detected in two of 80 samples (2.5%), and in these two cases, high-risk HPV was not detected in the uterine cervix or oral cavity of the child. It was concluded that the infection of HPV in maternal milk is rare (2/80); vertical transmission through maternal milk was not detected in this study (0/80). HPV infection through maternal milk may occur, but its likelihood is low.
(キーワード): Adult / Cervix Uteri / DNA Primers / DNA, Viral / Female / Human papillomavirus 16 / Humans / Infant / Infectious Disease Transmission, Vertical / Milk, Human / Mouth Mucosa / Papillomavirus Infections / Polymerase Chain Reaction
(徳島大学機関リポジトリ): ● Metadata: 113613
(出版サイトへのリンク): ● Publication site (DOI): 10.3109/01443615.2011.570814
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21823849; ● Search Scopus @ Elsevier (PMID): 21823849; ● Search Scopus @ Elsevier (DOI): 10.3109/01443615.2011.570814

(徳島大学機関リポジトリ: 113613, DOI: 10.3109/01443615.2011.570814, PubMed: 21823849) Sumika Matsui, Toshiyuki Yasui, Hirokazu Uemura, Satoshi Yamamoto, Toshiya Matsuzaki, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Induction of circulating monocyte chemoattractant protein-1 in women with gonadotropin-releasing hormone agonist.,
Journal of Reproductive Immunology, Vol.90, No.2, 227-234, 2011.

(要約): The aim of the present study was to determine the influence of acute estrogen deficiency induced by administration of a gonadotropin-releasing hormone (GnRH) agonist on circulating levels of cytokines and chemokines. Eighty-three women with uterine leiomyoma were assigned in open, parallel-group fashion to a no-treatment (control) group and a GnRH-agonist group. Serum levels of nine cytokines and chemokines as well as vascular inflammatory markers were measured. Serum levels of monocyte chemoattractant protein (MCP)-1 and tumor necrosis factor-α (TNFα) in the GnRH-agonist group were increased significantly at 6 months. There were also significant differences in percentage changes in interleukin (IL)-6, IL8, MCP1, and macrophage inflammatory protein-1β (MIP1β) between the control and GnRH agonist groups. Soluble intercellular adhesion molecule-1 (sICAM1) and E-selectin levels showed significant increases in the GnRH agonist group at 6 months. Serum MCP1 concentrations showed weak correlations with levels of sICAM and E-selectin. We conclude that a hypo-estrogenic state due to administration of a GnRH agonist increases circulating levels of cytokines and chemokines, especially MCP1.
(キーワード): Adult / Chemokine CCL2 / Cytokines / E-Selectin / Female / Fertility Agents, Female / Gonadotropin-Releasing Hormone / Humans / Intercellular Adhesion Molecule-1 / Leiomyoma / Leuprolide / Time Factors / Uterine Neoplasms
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jri.2011.04.011
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21726903; ● Search Scopus @ Elsevier (PMID): 21726903; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jri.2011.04.011

(DOI: 10.1016/j.jri.2011.04.011, PubMed: 21726903) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Riyo Kinouchi, Ganbat Gereltsetseg, Hiroshi Nakazawa, Satoshi Yamamoto, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Changes in responsiveness of appetite, leptin and hypothalamic IL-1β and TNF-α to lipopolysaccharide in developing rats.,
Journal of Neuroimmunology, Vol.236, No.1-2, 10-16, 2011.

(要約): In addition to its role as a regulator of energy homeostasis, leptin plays a pivotal role in some immune/inflammatory responses. Synthesis and secretion of leptin are increased under immune stress conditions, and increased leptin may participate in the development of anorexia and fever. These actions are partially mediated by up-regulation of hypothalamic IL-1β. Leptin also protects against immune stress-induced lethality. On the other hand, the response and roles of leptin to immune stress conditions in the neonatal period have scarcely been examined. We hypothesized that 1. the response of leptin to immune stress would be suppressed in the early neonatal period, 2. hyporesponse of leptin in the early neonatal period would attenuate the anorectic response and increase the lethal rate under immune stress conditions and 3. supplementation of leptin in the early neonatal period would increase the anorectic response, whereas it would decrease the lethal rate under immune stress conditions. To test these hypotheses, we first examined the developmental changes in the responses of leptin and hypothalamic proinflammatory cytokines, i.e., IL-1β and TNF-α, to LPS-induced immune stress in female rats. We also examined the developmental changes in the anorectic response and lethality rate under LPS-induced immune stress conditions. Five- and 15-day-old rats showed no leptin response and a weak hypothalamic IL-1β response to LPS when compared with 25- and 42-day-old rats. Fifteen-day-old rats showed low anorectic responses and high lethality rates when compared with 25- and 42-day-old rats under LPS-induced immune stress conditions. We then examined whether administration of leptin affected the response to the anorectic and lethal effects of LPS in 15-day-old rats. Administration of leptin further attenuated body weight after LPS injection, but not after saline injection. On the other hand, administration of leptin did not affect survival rate. In addition, hypothalamic IL-1β mRNA levels were not affected by leptin administration. In conclusion, the absence of a leptin response may act to prevent immune stress-induced anorexia during the early neonatal period.
(キーワード): Animals / Animals, Newborn / Appetite / Eating / Female / Hypothalamus / Interleukin-1beta / Leptin / Lipopolysaccharides / Pregnancy / RNA, Messenger / Rats / Rats, Sprague-Dawley / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jneuroim.2011.04.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21621857; ● Search Scopus @ Elsevier (PMID): 21621857; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jneuroim.2011.04.004

(DOI: 10.1016/j.jneuroim.2011.04.004, PubMed: 21621857) Souichiro Nakayama, Toshiyuki Yasui, Masanori Suto, Miki Sato, Takashi Kaji, Hirokazu Uemura, Kazuhisa Maeda and Minoru Irahara :
Differences in bone metabolism between singleton pregnancy and twin pregnancy.,
Bone, Vol.49, No.3, 513-519, 2011.

(要約): The objective of this study was to examine the influence of twin pregnancy on calcium metabolism, including bone turnover markers and calcium-regulating factors, by comparison between singleton pregnancy and twin pregnancy in women during pregnancy and puerperium in cross-sectional and longitudinal studies. Women with singleton and twin pregnancies were recruited from the outpatient clinic of Tokushima University Hospital. In both cross-sectional and longitudinal studies, bone formation and resorption markers, mineral metabolism and calcium-regulating factors were measured at 10, 25, 30 and 36 weeks of pregnancy and at 4 days and 1 month postpartum in women with singleton and twin pregnancies. Urinary levels of cross-linked type I collagen N-telopeptides and C-terminal telopeptides of type I collagen in women with twin pregnancy were significantly higher than those in women with singleton pregnancy and those high levels were observed earlier than those in women with singleton pregnancy. In the cross-sectional study, serum levels of bone-specific alkaline phosphatase, calcium and phosphate in women with twin pregnancy were higher and the levels of 1,25-(OH)2 vitamin D and 25-(OH) vitamin D in women with twin pregnancy were lower than those in women with singleton pregnancy. Changes in bone metabolism in women with twin pregnancy are different from those in women with singleton pregnancy. Early and large increases in bone turnover markers allow women with twin pregnancy to meet high fetal demand for calcium during pregnancy.
(キーワード): Adult / Alkaline Phosphatase / Biological Markers / Bone Remodeling / Bone and Bones / カルシウム (calcium) / Collagen Type I / Cross-Sectional Studies / Female / Gestational Age / Humans / Longitudinal Studies / Parathyroid Hormone / Peptide Fragments / Phosphates / Postpartum Period / Pregnancy / Pregnancy, Twin
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bone.2011.05.016
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21640859; ● Search Scopus @ Elsevier (PMID): 21640859; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bone.2011.05.016

(DOI: 10.1016/j.bone.2011.05.016, PubMed: 21640859) 笠井可菜, 加藤剛志, 谷口友香, 田中優, 阿部彰子, 海老沢桂子, 吉田加奈子, 古本博孝, 苛原稔 :
Leiomyomatosis peritonealis desseminata(LPD)の一例,
現代産婦人科, Vol.59, No.2, 271-273, 2011年.

(キーワード): 腹膜播種性平滑筋腫症 / 多発性子宮筋腫 / Leiomyomatosis peritoneal Desseminata / LPD / multiple myoma
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520009409867105536

(CiNii: 1520009409867105536) 吉田加奈子, 七條あつ子, 阿部彰子, 海老沢桂子, 加藤剛志, 古本博孝, 苛原稔 :
外陰のpapillary hidradenomaの1例,
現代産婦人科, Vol.59, No.2, 221-224, 2011年.

(キーワード): 外陰 / 乳頭状汗腺腫 / Vulva / Papillary hiradenoma
(徳島大学機関リポジトリ): ● Metadata: 113615
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050001338460975872

(徳島大学機関リポジトリ: 113615, CiNii: 1050001338460975872) Ayako Kobayashi, Masahiko Maegawa, Satoshi Yamamoto, Natsuyo Ugumori, Yuka Kasai, Anna Tani, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki, Toshiyuki Yasui, Hiroyuki Furumoto, Masaharu Kamada and Minoru Irahara :
The role of blood in early endometrial-peritoneal interactions in a syngeneic mouse model of endometriosis,
Reproductive Medicine and Biology, Vol.10, No.1, 15-20, 2011.

(キーワード): Blood / Coagulation / Endometriosis / Fibrinolysis / Murine model
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12522-010-0065-2
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1571417125008369024; ● Search Scopus @ Elsevier (DOI): 10.1007/s12522-010-0065-2

(DOI: 10.1007/s12522-010-0065-2, CiNii: 1571417125008369024) Akiko Abe, Hiroyuki Furumoto, Kanako Yoshida, Takeshi Katou, Yasuyo Saijo and Minoru Irahara :
Gene gun-mediated skin transfection with FL gene suppresses the growth of murine fibrosarcoma.,
The Journal of Medical Investigation : JMI, Vol.58, No.1-2, 39-45, 2011.

(要約): Particle-mediated transfection is known as an efficient method of non-viral gene transfer. Flt3 ligand (FL) is a growth factor for hematopoietic progenitors; it promotes the growth of dendritic cells (DC). DCs are powerful antigen-presenting cells (APCs) and show a remarkable capacity to stimulate antigen-specific T-cell responses. In this study, we intended to investigate the suppressive effect on tumor growth by gene gun-mediated transfer of FL in a murine model. C57BL/6J mice were injected intradermally with MCA205 cells. DNA (pNGVL-hFLex)-coated gold particles were delivered to the mouse skin surrounding the target tumor. The expression of FL was determined by RT-PCR. Analyses by immunohistochemistry and fluorescence-activated cell sorter (FACS) revealed an increase in the number of DC after treatment with FL. Gene gun-mediated pNGVL-hFLex transfer significantly inhibited the growth of the MCA205 tumor. FL transfer markedly increased the number of CD11c(+) DCs in the tumor tissue. Further, the FL-transfected mice exhibited a significantly higher number of CD80(+) MHC-II cells. We successfully performed FL therapy using an in vivo gene gun in order to effectively mobilize DCs in situ and induce suppressive immunity.
(キーワード): Animals / Base Sequence / Biolistics / Cell Line, Tumor / Dendritic Cells / 女性 (female) / Fibrosarcoma / 遺伝子発現 (gene expression) / 遺伝子治療 (gene therapy) / Membrane Proteins / Mice / Mice, Inbred C57BL / RNA, Messenger / Transfection
(徳島大学機関リポジトリ): ● Metadata: 83931
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.58.39
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21372485; ● Summary page in Scopus @ Elsevier: 2-s2.0-79951938597

(徳島大学機関リポジトリ: 83931, DOI: 10.2152/jmi.58.39, PubMed: 21372485, Elsevier: Scopus) Hiroaki Yanagawa, Minoru Irahara, Hitoshi Houchi, Yoshiyuki Kakehi, Takashi Moritoyo, Masahiro Nomoto, Mitsuhiko Miyamura and Taro Shuin :
View and present status of personnel involved in clinical trials: a survey of participants from the First Symposium of the Shikoku Collaborative Group for Promotion of Clinical Trials.,
The Journal of Medical Investigation : JMI, Vol.58, No.1-2, 81-85, 2011.

(要約): Clinical trials leading to drug approval (registration trials) play a central role in the drug development process. Since the introduction of the Good Clinical Practice (GCP) standard in 1997, the Japanese infrastructure for registration trials has improved. The contribution of support staff, including clinical research coordinators (CRCs), to clinical trials is now widely recognized in Japan. Quality issues and career development for these support staff are being increasingly emphasized. The Shikoku Collaborative Group for Promotion of Clinical Trials was organized in 2008 to address these issues through communication with the personnel involved in clinical trials in regional areas of Japan. To understand the views and present status of personnel involved in clinical trials, we used questionnaires to survey the participants of the First Symposium of the Shikoku Collaborative Group for Promotion of Clinical Trials held in August 2009. Group discussions and special lectures occurred at the symposium. The questionnaire began with questions about basic patient characteristics, followed by practical questions. Of 110 participants, there were 68 respondents (62%), including clinical trial support staff (clinical research coordinators [n=36, 53%], administrative officers [n=9, 13%]), and medical staff [n=23, 34%]). Among the support staff, 36 (80%) had more than 5 years of experience. The most common questionnaire answer selected for participation in the symposium was "willing to contact staff from other medical institutions or organizations" for support staff and "to obtain further knowledge concerning clinical trials" for medical staff. The overall view of the discussion ("Was the discussion satisfactory?") was favorable for 36 (53%) respondents. This survey revealed that the group discussion in the present symposium appears to be valuable for participants, using overall satisfaction as a surrogate. Based on the information obtained in the present study, further development of the clinical trial infrastructure, including training opportunities and career development for support staff, is required. Due to the limitations of this study, further analysis is warranted to determine the optimal strategy for training support staff.
(キーワード): Clinical Protocols / Clinical Trials as Topic / Cooperative Behavior / Drug Approval / Drug Discovery / Health Personnel / Humans / 日本 (Japan) / Questionnaires
(徳島大学機関リポジトリ): ● Metadata: 111305
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.58.81
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21372491; ● Search Scopus @ Elsevier (PMID): 21372491; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.58.81

(徳島大学機関リポジトリ: 111305, DOI: 10.2152/jmi.58.81, PubMed: 21372491) Yuka Kasai, Masahiko Maegawa, Satoshi Yamamoto, Masaharu Kamada, Toshiyuki Yasui, Hirokazu Uemura, Ayako Kobayashi, Masayo Kaneyama, Anna Tani, Sumika Matsui, Akira Kuwahara, Toshiya Matsuzaki, Hiroyuki Furumoto and Minoru Irahara :
Effects of raloxifene on the production of cytokines in stimulated whole blood in ex vivo and in vitro studies.,
The Journal of Medical Investigation : JMI, Vol.58, No.1-2, 110-117, 2011.

(要約): The aims of this study were to determine the effects of raloxifene therapy on production of cytokines and in vitro effects of raloxifene on production of cytokines by whole blood cultures. We obtained samples of peripheral blood from 6 postmenopausal women with osteopenia at baseline and after 3 and 6 months of raloxifene therapy and 10 postmenopausal women who did not receive raloxifene therapy. Whole blood from raloxifene-treated women was stimulated with lipopolysaccharide (LPS) or phytohemeagglutinin (PHA). Whole blood from postmenopausal women who were not treated with raloxifene was preincubated with raloxifene at concentrations of 10(-10)-10(-7) M and then stimulated with LPS or PHA. Concentrations of IL-1β, IL-4, IL-6, IL-12p40, IL-12p70, TNF-α and IFN-γ in the supernatant were measured by respective ELISAs. In ex vivo cultures, raloxifene therapy inhibited LPS-stimulated production of IL-1β, IL-6, IL-12p40, IL-12p70 and TNF-α, but not PHA-stimulated production of IL-4 and IFN-γ. In in vitro cultures, raloxifene at a concentration (10(-9) M) inhibited LPS-stimulated production of IL-1β, IL-6 and IL-12p40 and PHA-stimulated production of IFN-γ. Raloxifene therapy decreases the production of IL-1β, IL-6, IL-12 and TNF-α but not that of IL-4 and IFN-γ, suggesting that modulation of cytokines could play a role in the mechanisms of the osteoprotective effect of raloxifene.
(キーワード): Aged / Bone Density Conservation Agents / Bone Diseases, Metabolic / Cell Survival / Cytokines / Female / Humans / Leukocytes, Mononuclear / Menopause / Middle Aged / Raloxifene / Selective Estrogen Receptor Modulators / Time Factors
(徳島大学機関リポジトリ): ● Metadata: 80825
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.58.110
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21372495; ● Search Scopus @ Elsevier (PMID): 21372495; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.58.110

(徳島大学機関リポジトリ: 80825, DOI: 10.2152/jmi.58.110, PubMed: 21372495) Toshiyuki Yasui, Sumika Matsui, S Yamamoto, Hirokazu Uemura, N Tsuchiya, M Noguchi, M Yuzurihara, Y Kase and Minoru Irahara :
Effects of Japanese traditional medicines on circulating cytokine levels in women with hot flashes.,
Menopause, Vol.18, No.1, 85-92, 2011.

(要約): The effects of the Japanese traditional medicines keishibukuryogan and kamishoyosan on circulating cytokines were examined to clarify the difference in the actions of Japanese traditional medicines in women with hot flashes. Seven premenopausal, 51 perimenopausal, 45 spontaneously postmenopausal and 17 surgically postmenopausal women who had complained of hot flashes were enrolled in this study. Eighty women who hoped to receive Japanese traditional medicines were randomly assigned in open, parallel-group fashion to a keishibukuryogan group or kamishoyosan group. Forty women who did not want any treatment for hot flashes were followed up for 6 months as a control group. Serum levels of cytokines were measured using a multiplexed human cytokine assay. The proportions of responders in women treated with keishibukuryogan and kamishoyosan were 73.7% and 69.2%, respectively. Serum monocyte chemotactic protein-1 level in women treated with keishibukuryogan decreased significantly (P = 0.0037). On the other hand, concentrations of interleukin (IL)-6 and macrophage inflammatory protein-1β in women treated with kamishoyosan decreased significantly (P = 0.019 and P = 0.039, respectively). In both keishibukuryogan and kamishoyosan responder groups, serum IL-8 concentrations were reduced significantly (P = 0.021 and P = 0.014, respectively). Both treatments with keishibukuryogan and kamishoyosan reduce the circulating IL-8 level, which is involved in thermoregulation in perimenopausal women with hot flashes. In addition, keishibukuryogan decreases circulating monocyte chemotactic protein-1 level in postmenopausal women.
(キーワード): Adult / Chemokine CCL2 / Drugs, Chinese Herbal / Estradiol / Female / Follicle Stimulating Hormone / Hot Flashes / Humans / Interleukins / Medicine, Traditional / Menopause / Middle Aged / Postmenopause / Premenopause / Statistics, Nonparametric / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/gme.0b013e3181e5063c
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20647958; ● Search Scopus @ Elsevier (PMID): 20647958; ● Search Scopus @ Elsevier (DOI): 10.1097/gme.0b013e3181e5063c

(DOI: 10.1097/gme.0b013e3181e5063c, PubMed: 20647958) 吉田加奈子, 加藤剛志, 田中優, 山本哲史, 桑原章, 松崎利也, 苛原稔 :
当科における子宮内膜症性嚢胞を伴う不妊症患者に対する手術療法の検討,
日本エンドメトリオーシス学会会誌, Vol.32, 106-108, 2011年.

(徳島大学機関リポジトリ): ● Metadata: 113616
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050564288781197184

(徳島大学機関リポジトリ: 113616, CiNii: 1050564288781197184) 菊地久美子, 友竹正人, 伊賀淳一, 上野修一, 苛原稔, 大森哲郎 :
周産期の抑うつに影響を与える心理特性に関する検討,
臨床精神医学, Vol.39, No.11, 1459-1468, 2010年.

(キーワード): 周産期 / perinatal period / 抑うつ / depressive state / 心理特性 / psychological features / EPDS / Edinburgh Postnatal Depression Scale
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523951030100974464

(CiNii: 1523951030100974464) Akiko Abe, Hiroyuki Furumoto, Minoru Irahara, Hiroyasu Ino, Masaharu Kamada, Osamu Naka, Masaru Sasaki, Toshiaki Kagawa, Osamu Okitsu and Norio Kushiki :
The impact of systematic para-aortic and pelvic lymphadenectomy on survival in patients with optimally debulked ovarian cancer.,
The Journal of Obstetrics and Gynaecology Research, Vol.36, No.5, 1023-1030, 2010.

(要約): The objective of this study was to verify the impact of systematic retroperitoneal lymphadenectomy on survival in patients with ovarian cancer.
(キーワード): 成人教育経営 (adult education administration) / 乳がん (breast carcinoma) / Female / Humans / Kaplan-Meier Estimate / Lymph Node Excision / Ovarian Neoplasms / Regression Analysis / Retroperitoneal Space / Survival Rate / Treatment Outcome
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1447-0756.2010.01274.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21058437; ● CiNii @ 国立情報学研究所 (CRID): 1571135651142514816; ● Search Scopus @ Elsevier (PMID): 21058437; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1447-0756.2010.01274.x

(DOI: 10.1111/j.1447-0756.2010.01274.x, PubMed: 21058437, CiNii: 1571135651142514816) 毛山薫, 加地剛, 村上雅博, 須藤真功, 佐藤美紀, 前田和寿, 苛原稔 :
胎児診断した潜在性二分脊椎の一例,
現代産婦人科, Vol.59, No.1, 23-27, 2010年.

(キーワード): 潜在性二分脊椎 / 脊髄脂肪腫 / 胎児診断 / closed spina bifida / spinal lipoma / prenatal diagnosis
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572359823782272

(CiNii: 1520572359823782272) 吉田加奈子, 加藤剛志, 山本由理, 田中優, 苛原稔 :
骨盤内膿瘍に対する腹腔鏡手術の有用性,
現代産婦人科, Vol.59, No.1, 59-63, 2010年.

(要約): 骨盤内膿瘍に対する治療は，抗生剤による薬物療法が主体であるが，抗生剤無効例や，消化管から発生した膿瘍との鑑別を要する症例では外科的アプローチが必要となる．骨盤内膿瘍の場合，炎症による癒着が強いことが多く，手術は比較的難易度が高い．しかし，高度の炎症で疲弊した患者の負担を軽減するために，近年では腹腔鏡手術が積極的に導入されており，当院でも可能な限り腹腔鏡手術で対応している．抗生剤による治療が無効であった付属器膿瘍に対して腹腔鏡下手術を施行した4症例について報告する．4例とも輸血を要するような出血はなく，また他臓器損傷もなかった．術後は，速やかに炎症所見が改善し特に問題なく経過した．骨盤内膿瘍に対する腹腔鏡手術は，ドレナージによりすみやかな炎症軽減が可能であり，かつ低侵襲であることから有用性は高い．一方で，(汎発性腹膜炎などで，)腸管麻痺を伴う症例では，腸管の膨隆のため腹腔鏡下の視野確保が困難であり，腹腔鏡手術の適応は慎重であるべきであると考える．
(キーワード): 付属器膿瘍 / 腹腔鏡 / tubo-ovaruan abscess / laparoscopic surgery
(徳島大学機関リポジトリ): ● Metadata: 113614
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050282813437686400

(徳島大学機関リポジトリ: 113614, CiNii: 1050282813437686400) 柴田真紀, 加藤剛志, 吉田加奈子, 佐藤美紀, 田中優, 苛原稔 :
妊娠初期に捻転を起こし腹腔鏡下手術を行ったmassive ovarian edema の一例,
現代産婦人科, Vol.59, No.1, 55-57, 2010年.

(キーワード): 急性腹症 / Massive ovarian edema torsion laparoscopy
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520009409865699968

(CiNii: 1520009409865699968) Yuji Taketani, Kelly Eduardo, Yoshimura Yasunori, Hoshiai Hiroshi, Minoru Irahara, Mizunuma Hideki, Saito Hidekazu, Andoh Kazumichi, Bebia Zourab and Yanaihara Takumi :
Recombinant follicle-stimulating hormone (follitropin alfa) for ovulation induction in Japanese patients with anti-estrogen-ineffective oligo- or anovulatory infertility: results of a phase II dose response study,
Reproductive Medicine and Biology, Vol.9, No.2, 91-97, 2010.

(キーワード): Amenorrhea / Chronic low-dose step-up regimen / Follitropin alfa / Ovulation induction / Recombinant human follicle-stimulating hormone
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12522-009-0044-7
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1572543026108013312; ● Search Scopus @ Elsevier (DOI): 10.1007/s12522-009-0044-7

(DOI: 10.1007/s12522-009-0044-7, CiNii: 1572543026108013312) 木内理世, 松崎利也, 岩佐武, 三室卓久, 苛原稔 :
エクルーシス試薬テストステロンⅡ,LH,FSHの基準値の検討,
医学と薬学, Vol.64, No.1, 87-93, 2010年. Hiroaki Yanagawa, Masatoshi Kishuku, Masashi Akaike, Hiroyuki Azuma and Minoru Irahara :
View of physicians on and barriers to patient enrollment in a multicenter clinical trial: experience in a Japanese rural area,
International Archives of Medicine, Vol.3, No.1, 7, 2010.

(要約): Clinical trials in the general practice setting are important for providing evidence on the effectiveness and safety of different agents under various conditions. In conducting these trials, the participation of physicians and patient recruitment are important issues. Various investigations in the literature have reported views and attitudes of physicians on various types of clinical trials. Nevertheless, there is still little information concerning physicians participating in a clinical trial and among them, those who could not recruit any patients (unsuccessful physician recruiters). In 2003, we collaborated in a large-scale multicenter study of Japanese hypertensive patients (COPE Trial). In Tokushima University Hospital and 18 other medical institutions, we investigated the views and attitudes of unsuccessful physician recruiters in comparison with successful physician recruiters, using a questionnaire. The questionnaire was provided by mail to 47 physicians and 27 (57%) responded. The response rate was 79% for successful physician recruiters compared to 43% (P = 0.014) for unsuccessful physician recruiters. More successful physician recruiters (73%) than unsuccessful physician recruiters (42%) stated they had participated and enrolled patients in previous multicenter clinical trials. A significantly higher number of successful physician recruiters than unsuccessful physician recruiters (42%; P = 0.040) considered the presence of a support system with clinical research coordinators (CRC) as the reason for participation (80%). A large number of unsuccessful physician recruiters experienced difficulty in obtaining informed consent (67%), whereas a significantly smaller number of successful physician recruiters experienced such difficulty (20%; P = 0.014). The difficulties experienced by unsuccessful physician recruiters in the trial were as follows: inability to find possible participants (100%), difficulty in obtaining informed consent (58%), cumbersome procedures (58%), difficulty in long-term follow up (33%), and insufficient tools for explanation and obtaining informed consent (8%). This survey showed that successful physician recruiters consider a support system with CRC of value, and that they are skillful in obtaining informed consent. These views and attitudes may have originated from past experience involving clinical trials. In this regard, we need to develop an infrastructure to enlighten physicians on this support system for the promotion of clinical trials.
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/1755-7682-3-7
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20525325; ● Search Scopus @ Elsevier (PMID): 20525325; ● Search Scopus @ Elsevier (DOI): 10.1186/1755-7682-3-7

(DOI: 10.1186/1755-7682-3-7, PubMed: 20525325) Hiroaki Yanagawa, Junji Terao, Eiji Takeda, Yoshihisa Takaishi, Yoshiki Kashiwada, Kazuyoshi Kawazoe, Fushitani Shuji, Koichiro Tsuchiya, Aiko Yamauchi, Sato Chiho and Minoru Irahara :
Consultation clinics for complementary and alternative medicine at Japanese university hospitals: An analysis at Tokushima University Hospital,
Experimental and Therapeutic Medicine, Vol.1, No.3, 481-483, 2010.

(要約): Here, we report on a Consultation Clinic for Complementary and Alternative Medicine (CAM) which we established at Tokushima University Hospital in July of 2007 with the aim of providing person-to-person information on CAM, though not CAM therapy itself. In December of 2008, we received 55 applications for consultation, 37% concerning health foods, 37% Japanese herbal medicine (Kampo), and 26% various other topics. The consultants (nutritionists and pharmacists) communicated individually with 38 applicants; malignancies (26%) and cardiovascular disease (24%) were the main underlying concerns. To promote the quality of consultation, data was collected by means of focus group interviews concerning the perspective of the consultants. Safe and effective use of CAM requires a network of communication linking individuals, consultation teams, physicians, primary care institutions and university hospitals. To advance this goal, we plan to broaden the efforts described herein. Our findings indicate that the specific role of the consultation clinic in promoting the scientific use of CAM merits further study.
(出版サイトへのリンク): ● Publication site (DOI): 10.3892/etm_00000075
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22993564; ● Search Scopus @ Elsevier (PMID): 22993564; ● Search Scopus @ Elsevier (DOI): 10.3892/etm_00000075

(DOI: 10.3892/etm_00000075, PubMed: 22993564) Takeshi Iwasa, Toshiya Matsuzaki, Riyo Kinouchi, Shinobu Fujisawa, Masahiro Murakami, Machiko Kiyokawa, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Neonatal LPS injection alters the body weight regulation systems of rats under non-stress and immune stress conditions.,
International Journal of Developmental Neuroscience, Vol.28, No.1, 119-124, 2010.

(要約): It has been reported that prenatal immune stress induced by lipopolysaccharides or cytokines increases food intake and leads to obesity and other features of metabolic syndrome in adulthood. Using Sprague-Dawley rats, we evaluated whether neonatal LPS injection altered their body weight regulation systems under non-stress and immune stress conditions. On Day 10 after birth, all pups were injected with LPS (100 microg/kg, i.p.) (PND(10)LPS) or saline (PND(10)Saline). After weaning, body weight was significantly elevated in PND(10)LPS compared with PND(10)Saline. Thereafter, the rats were injected with LPS (100 microg/kg, i.p.) or saline (used as a basal condition) from 7 to 8 weeks of age. Under basal conditions, cumulative food intake were significantly higher, serum leptin concentration was significantly increased, and hypothalamic NPY mRNA expression was significantly decreased in PND(10)LPS compared with PND(10)Saline. Under adult LPS injected conditions, body weight gain and cumulative food intake were suppressed in both the PND(10)LPS and PND(10)Saline groups compared with those observed under basal adult saline-injected conditions. The suppressive effects induced by adult LPS injection were less evident in the PND(10)LPS group than in the PND(10)Saline group. Adult LPS injection increased the serum leptin concentration in the PND(10)Saline rats, but not in the PND(10)LPS rats. In addition, adult LPS injection increased the mRNA expression of anorexinergic factors (IL-1beta, and TNF-alpha), and decreased that of the orexinergic factor NPY in both groups. However, the influence of adult LPS injection upon these factors was less evident in the PND(10)LPS group than in the PND(10)Saline group. These results suggest that neonatal LPS injection alters body weight regulation under both non-stress and immune stress conditions in male rats. Changes in the endocrine, neuropeptide, and cytokine regulation systems might be involved in these alterations.
(キーワード): Aging / Animals / Animals, Newborn / Body Weight / Corticosterone / Feeding Behavior / 視床下部 (hypothalamus) / Interleukin-1beta / Leptin / Lipopolysaccharides / Male / Neuropeptide Y / RNA, Messenger / Random Allocation / Rats / Rats, Sprague-Dawley / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2009.08.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19733650; ● Search Scopus @ Elsevier (PMID): 19733650; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2009.08.015

(DOI: 10.1016/j.ijdevneu.2009.08.015, PubMed: 19733650) Toshiyuki Yasui, Sumika Matsui, Ayako Saijo, Takashi Kaji, Satoshi Yamamoto, Hirokazu Uemura, Toshiya Matsuzaki and Minoru Irahara :
Association of N-terminal pro B-type natriuretic peptide and sex hormone-binding globulin in non-obese peri- and postmenopausal women.,
Clinica Chimica Acta, Vol.411, No.3-4, 280-284, 2010.

(要約): BACKGROUND: The relationships between N-terminal fragment proBNP (B-type natriuretic peptide) (NT-proBNP) and sex steroid hormones have not been fully elucidated. We examined these associations in pre-, peri- and postmenopausal women without known cardiovascular disease. We also examined the change in circulating NT-proBNP caused by a gonadotrophin-releasing hormone (GnRH) agonist in order to clarify the effect of acute hormone deficiency on NT-proBNP. METHODS: Forty-nine premenopausal women, 101 perimenopausal women and 108 postmenopausal women were enrolled in one study. Forty-four premenopausal women with leiomyoma were recruited for another study, and 22 of those women were treated with a GnRH agonist. Serum levels of NT-proBNP, estradiol, testosterone and sex hormone-binding globulin (SHBG) were measured. RESULTS: NT-proBNP concentrations showed significant inverse correlations with body mass index (BMI) in peri- and postmenopausal women. Serum NTproBNP concentrations showed significant positive correlations with SHBG in peri- and postmenopausal women. These significant correlations remained after adjustment for age and BMI. Serum NT-proBNP concentration was significantly decreased after GnRH agonist administration, while NT-proBNP concentration in the control group did not change significantly. Serum SHBG level was also significantly decreased after GnRH agonist administration. CONCLUSION: Circulating NT-proBNP levels are positively associated with SHBG levels in non-obese peri- and postmenopausal women, and this relationship was found under the condition of acute hormone deficiency induced by GnRH agonist treatment.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cca.2009.11.025
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19945451; ● Search Scopus @ Elsevier (PMID): 19945451; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cca.2009.11.025

(DOI: 10.1016/j.cca.2009.11.025, PubMed: 19945451) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Shinobu Fujisawa, Riyo Kinouchi, Ganbat Gereltsetseg, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Effects of intrauterine undernutrition on hypothalamic Kiss1 expression and the timing of puberty in female rats.,
The Journal of Physiology, Vol.588, No.Pt 5, 821-829, 2010.

(要約): Recent studies have suggested that intrauterine undernutrition is closely associated with the pathogenesis of diseases after birth. Perinatal undernutrition is known to disturb the development of reproductive function and delay the onset of puberty in some species. Using a rat model, we determined the effects of prenatal undernutrition on the development of the hypothalamic kisspeptin system and evaluated whether the alteration of the kisspeptin system contributes to the delayed onset of puberty induced by prenatal undernutrition. We also evaluated the effects of prenatal undernutrition on the developmental changes in serum leptin levels because leptin was a putative positive regulator of the hypothalamic kisspeptin system. We compared the timing of vaginal opening (VO) and the developmental changes in body weight, hypothalamic Kiss1 mRNA levels, and serum leptin concentrations between offspring with prenatal undernutrition (UN offspring) and normal nutrition (NN offspring). After birth, the UN offspring showed rapid growth and had caught up to body weight of the NN offspring by postnatal day 12. After postnatal day 16, the UN offspring showed significantly lower Kiss1 mRNA levels than the NN offspring, despite their significantly higher serum leptin levels (at days 20 and 28). The timing of VO in the UN offspring was delayed compared with that in the NN offspring, and chronic central injection of kisspeptin normalized the timing of VO in the UN offspring. These results suggest that decreased hypothalamic kisspeptin action contributes to the delayed onset of puberty in prenatally undernourished female rats. Increased leptin resistance in the kisspeptin system might be involved in these alterations.
(キーワード): Animals / Female / 視床下部 (hypothalamus) / Malnutrition / Proteins / Puberty / Rats / Rats, Sprague-Dawley
(出版サイトへのリンク): ● Publication site (DOI): 10.1113/jphysiol.2009.183558
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20083512; ● Search Scopus @ Elsevier (PMID): 20083512; ● Search Scopus @ Elsevier (DOI): 10.1113/jphysiol.2009.183558

(DOI: 10.1113/jphysiol.2009.183558, PubMed: 20083512) Rie Ogata, Toshiya Matsuzaki, Takeshi Iwasa, Machiko Kiyokawa, Naoko Tanaka, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Hypothalamic Ghrelin suppresses pulsatile secretion of luteinizing hormone via beta-endorphin in ovariectomized rats.,
Neuroendocrinology, Vol.90, No.4, 364-370, 2009.

(要約): OBJECTIVES: Ghrelin, an endogenous growth hormone secretagogue, is a known accelerator of feeding behavior and suppresses pulsatile secretion of luteinizing hormone (LH) in ovariectomized rats. However, the mechanisms underlying this action remain unclear. We examined the effects of naloxone (NAL), a specific opioid antagonist, on the suppression of pulsatile LH secretion by ghrelin to determine whether beta-endorphin (beta-END) is involved in this suppressive effect. METHODS: Ghrelin was administered intracerebroventricularly, and NAL was injected intravenously in ovariectomized rats; then, serum LH concentrations were measured by radioimmunoassay in blood samples drawn every 6 min for 2 h to analyze pulsatile secretion. RESULTS: Administration of ghrelin significantly reduced mean LH concentration and pulse frequency. Coadministration of NAL with ghrelin significantly restored mean LH concentration and pulse frequency. CONCLUSION: Suppressive effect of intracerebroventricular injection of ghrelin on pulsatile LH secretion was mediated by beta-END, suggesting that hypothalamic ghrelin suppressed pulsatile gonadotropin-releasing hormone secretion via beta-END in female rats.
(キーワード): Animals / Body Weight / Catheterization / Female / Ghrelin / 視床下部 (hypothalamus) / Luteinizing Hormone / Naloxone / Narcotic Antagonists / Ovariectomy / Radioimmunoassay / Rats / Rats, Wistar / Time Factors / beta-Endorphin
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000257421
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19907132; ● Search Scopus @ Elsevier (PMID): 19907132; ● Search Scopus @ Elsevier (DOI): 10.1159/000257421

(DOI: 10.1159/000257421, PubMed: 19907132) Toshiyuki Yasui, Ayako Saijo, Hirokazu Uemura, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Interleukin-7 is associated with monocyte chemoattractant protein-1 and soluble E-selectin levels in peripheral blood of newly post-menopausal women.,
Journal of Reproductive Immunology, Vol.81, No.1, 97-102, 2009.

(要約): Interleukin (IL-7), which is a regulator of development and homeostatic maintenance of T and B cells, is implicated in the induction of leukocyte-endothelial cell adhesion during inflammatory events. We hypothesized that circulating IL-7 is associated with cytokines and chemokines, vascular inflammatory markers and lipid profiles involved in regulating cell adhesion in post-menopausal women. Serum levels of 8 cytokines and chemokines were simultaneously measured in 200 post-menopausal women using a multiplexed human cytokine assay. C-reactive protein, soluble vascular cell adhesion molecule, soluble intercellular adhesion molecule and soluble E-selectin were measured as inflammatory markers. Serum IL-7 concentration was significantly correlated with serum MCP-1 concentration and the correlation remained after adjustment for age and body mass index (BMI). Serum IL-7 levels also showed significant positive correlations with serum levels of IL-10 and IL-6. In addition, the serum IL-7 concentration showed significant positive correlations with levels of soluble E-selectin and triglyceride after adjustment for age and BMI. We conclude that there is a relationship between circulating IL-7, MCP-1 and soluble E-selectin, and that IL-7 may be involved in regulating inflammatory cell adhesion together with MCP-1 and E-selectin in post-menopausal women.
(キーワード): Age Factors / Body Mass Index / C-Reactive Protein / Cell Adhesion / Chemokine CCL2 / E-Selectin / Female / Humans / Inflammation / Intercellular Adhesion Molecule-1 / Interleukin-10 / Interleukin-6 / Interleukin-7 / Middle Aged / Postmenopause / Triglycerides / Vascular Cell Adhesion Molecule-1 / Vascular Diseases
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jri.2009.04.006
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19501916; ● Search Scopus @ Elsevier (PMID): 19501916; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jri.2009.04.006

(DOI: 10.1016/j.jri.2009.04.006, PubMed: 19501916) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Fumi Shimizu, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Reproducibility of luteinizing hormone hypersecretion in different phases of the menstrual cycle in polycystic ovary syndrome.,
The Journal of Obstetrics and Gynaecology Research, Vol.35, No.3, 514-519, 2009.

(要約): AIM: We evaluated the reproducibility of an elevated luteinizing hormone (LH) level or elevated LH/follicle-stimulating hormone (FSH) ratio in different phases of the menstrual cycle in patients with polycystic ovary syndrome (PCOS). Furthermore, we evaluated the influence of cycle days and body mass index on LH levels using different days from menses or withdrawal bleeding. METHODS: Forty-three patients with PCOS and 37 controls were enrolled. All patients with PCOS had elevated LH levels and LH/FSH ratio, polycystic ovarian morphology and chronic anovulation at diagnosis. After their diagnoses, we measured serum LH and FSH in the early period and/or late period of the follicular phase. RESULTS: The rate of patients with elevated LH levels and LH/FSH ratio were significantly higher in the late phase than in the early phase in PCOS. In the early phase, only 52% of patients had elevated LH levels and LH/FSH ratio. We failed to demonstrate an inverse relationship between the LH level and BMI. Conclusions: LH levels of patients with PCOS were strongly influenced by the days from menses or withdrawal bleeding. In the early period, the reproducibility of elevated LH levels or LH/FSH ratio was poor even in patients previously diagnosed with them.
(キーワード): Adult / Body Mass Index / Female / Follicle Stimulating Hormone / Humans / Luteinizing Hormone / Menstrual Cycle / Polycystic Ovary Syndrome
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1447-0756.2008.00998.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19527392; ● CiNii @ 国立情報学研究所 (CRID): 1573105975710832640; ● Search Scopus @ Elsevier (PMID): 19527392; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1447-0756.2008.00998.x

(DOI: 10.1111/j.1447-0756.2008.00998.x, PubMed: 19527392, CiNii: 1573105975710832640) Toshiyuki Yasui, Ayako Saijo, Hirokazu Uemura, Toshiya Matsuzaki, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Effects of oral and transdermal estrogen therapies on circulating cytokines and chemokines in postmenopausal women with hysterectomy.,
European Journal of Endocrinology, Vol.161, No.2, 267-273, 2009.

(要約): OBJECTIVE: The aim of the present study was to determine the different effects of oral estrogen therapy (ET) and transdermal ET on changes in circulating levels of cytokines and chemokines in relationship to changes in markers of inflammation in postmenopausal women with hysterectomy. METHODS: Fifty-five postmenopausal women with hysterectomy were randomly assigned in open, parallel-group fashion to an oral ET group and a transdermal ET group. Serum levels of cytokines and chemokines were simultaneously measured using a multiplexed human cytokine assay. Serum concentrations of high-sensitive C-reactive protein, soluble vascular cell adhesion molecule-1, soluble intercellular adhesion molecule-1, and E-selectin were measured as vascular inflammation markers. RESULTS: Both oral ET and transdermal ET significantly decreased serum interleukin (IL)-7 concentrations at 12 months (P=0.020 and P=0.015 respectively). Transdermal ET decreased serum concentrations of IL-8, monocyte chemoattractant protein (MCP)-1, and macrophage inflammatory protein (MIP)-1beta (P=0.05, P=0.019, and P=0.029), but oral ET increased IL-8 level (P=0.025). There were significant differences in percentage changes in IL-8 and MIP-1beta between the oral and transdermal ET groups. Oral ET significantly decreased E-selectin level after 12 months. CONCLUSION: Transdermal ET reduces circulating levels of IL-8, MCP-1, and MIP-1beta, while both oral ET and transdermal ET reduce circulating level of IL-7.
(キーワード): Administration, Cutaneous / Administration, Oral / C-Reactive Protein / Chemokines / Cytokines / E-Selectin / Estrogen Replacement Therapy / Estrogens / Female / Humans / Hysterectomy / Inflammation / Intercellular Adhesion Molecule-1 / Middle Aged / Postmenopause / Statistics, Nonparametric / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1530/EJE-09-0063
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19451213; ● Search Scopus @ Elsevier (PMID): 19451213; ● Search Scopus @ Elsevier (DOI): 10.1530/EJE-09-0063

(DOI: 10.1530/EJE-09-0063, PubMed: 19451213) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Riyo Kinouchi, Rie Ogata, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Neonatal lipopolysaccharide exposure attenuates the homotypic stress-induced suppression of LH secretion in adulthood in male rat.,
International Journal of Developmental Neuroscience, Vol.27, No.4, 345-349, 2009.

(要約): Neonatal immune challenges have a long-lasting influence on immune response. Using male Sprague-Dawley rats, we examined whether neonatal lipopolysaccharide (LPS) challenge alters the sensitivity of male reproductive function to adult LPS challenge and at which level (central or testes) the alteration occurs. We also examined the mRNA expression of proinflammatory cytokines in the hypothalamus and testes because they have a pivotal role in immune stress-induced suppression of gonadotropin secretion and testosterone synthesis. On day 10 after birth, all the pups were injected with LPS (100 microg/kg, i.p.) or saline. Thereafter, LPS (100 microg/kg, i.p.) or saline was injected in adulthood at 8 weeks of age. The serum LH concentration was decreased by LPS injection during adulthood in the neonatal saline-injected rats. This suppressive effect was not seen in the neonatal LPS-injected rats. The serum testosterone concentration was decreased by adult LPS injection in both the neonatal LPS-injected and neonatal saline-injected rats. The expression levels of KiSS-1, which encodes kisspeptin, known to have a crucial role in the regulation of gonadotropin secretion, and GnRH mRNA in the hypothalamus and LHbeta mRNA in the pituitary were not influenced by neonatal or adult LPS injection. On the other hand, the expression levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) mRNA in the hypothalamus and testes were increased by adult LPS injection in both the neonatal LPS-injected and neonatal saline-injected rats. Furthermore, the expression levels of these factors in the hypothalamus after adult LPS injection were significantly lower in the neonatal LPS-injected rats than in the neonatal saline-injected rats. These findings indicate that neonatal LPS challenge reduces the sensitivity of male reproductive function to the suppressive effects of LPS, mainly at the central level. Attenuation of proinflammatory cytokine synthesis in the hypothalamus might be involved in this alteration.
(キーワード): Animals / Animals, Newborn / Female / 視床下部 (hypothalamus) / Interleukin-1beta / Lipopolysaccharides / Luteinizing Hormone / Male / 下垂体 (pituitary gland) / Pregnancy / Rats / Rats, Sprague-Dawley / Stress, Physiological / Testis / Testosterone / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2009.03.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19460628; ● Search Scopus @ Elsevier (PMID): 19460628; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2009.03.004

(DOI: 10.1016/j.ijdevneu.2009.03.004, PubMed: 19460628) Toshiyuki Yasui, Masayo Yamada, Hirokazu Uemura, Shu-Ichi Ueno, Shusuke Numata, Tetsuro Ohmori, Naoko Tsuchiya, Masamichi Noguchi, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Changes in circulating cytokine levels in midlife women with psychological symptoms with selective serotonin reuptake inhibitor and Japanese traditional medicine.,
Maturitas, Vol.62, No.2, 146-152, 2009.

(要約): OBJECTIVE: The aim of the present study was to compare the effects on serum cytokine concentrations of paroxetine, a selective serotonin re-uptake inhibitor, and kamishoyosan, a Japanese traditional medicine, in midlife women with psychological symptoms. METHODS: Seventy-six women with psychological symptoms such as anxiety and mild depression as menopausal symptoms were enrolled in this study. Thirty-eight women received oral administration of 10mg paroxetine every day, and 38 women received oral administration of kamshoyosan every day for 6 months. Overall climacteric symptoms were assessed using Greene's climacteric scale. Serum levels of cytokines were measured using a multiplexed human cytokine assay. RESULTS: Greene's total scores in both women treated with paroxetine and in women treated with kamishoyosan decreased significantly. Percentage decreases in Greene's total, psychological and vasomotor scores during the 6-month period in the paroxetine group were significantly greater than those in the kamishoyosan group. Serum IL-6 concentration in women treated with paroxetine decreased significantly. Serum concentrations of IL-8, IL-10, macrophage inflammatory protein (MIP)-1beta and monocyte chemoattractant protein-1 in women treated with paroxetine decreased significantly. On the other hand, serum IL-6 concentration in women treated with kamishoyosan decreased significantly, but other serum concentrations did not change significantly. CONCLUSION: Decrease in IL-6 concentration may be involved in the mechanism of the actions of both paroxetine and kamishoyosan in women with psychological symptoms, and IL-6 may therefore be useful as a marker of treatment. The action of paroxetine may also be associated with decreases in IL-8, IL-10, MIP-1beta.
(キーワード): Administration, Oral / Angiosperms / Climacteric / Cytokines / Depression / Drugs, Chinese Herbal / Hot Flashes / Humans / Medicine, East Asian Traditional / Middle Aged / Paroxetine / Phytotherapy / Plant Extracts / Serotonin Uptake Inhibitors
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.maturitas.2008.12.007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19179025; ● Search Scopus @ Elsevier (PMID): 19179025; ● Search Scopus @ Elsevier (DOI): 10.1016/j.maturitas.2008.12.007

(DOI: 10.1016/j.maturitas.2008.12.007, PubMed: 19179025) Toshiyuki Yasui, Hirokazu Uemura, S Hyodo, M Yamada, Satoshi Yamamoto, M Maegawa, N Tsuchiya, M Noguchi, M Yuzurihara, Y Kase and Minoru Irahara :
Raloxifene reduces circulating levels of interleukin-7 and monocyte chemoattractant protein-1 in postmenopausal women.,
Atherosclerosis, 2009.

(要約): OBJECTIVE: The aim of the present study was to determine the effects of raloxifene on changes in circulating levels of cytokines and chemokines in relation to changes in lipid profiles and markers of inflammation in postmenopausal women. METHODS: Fifty-three postmenopausal women aged 45-65 years old were randomly assigned in open, parallel-group fashion to a control group or raloxifene group. Twenty-six women received oral administration of 60mg raloxifene every day and 27 women did not receive any drugs for 12 months. Serum cytokines levels were simultaneously measured using a multiplexed human cytokine assay. RESULTS: Serum IL-7 concentrations in women who received raloxifene were decreased significantly (p=0.014), and serum monocyte chemoattractant protein (MCP)-1 concentrations in women who received raloxifene were decreased significantly (p=0.0003) at 12 months. In the control group, serum levels of MCP-1 and IL-7 did not show significant changes. There were significant differences (p=0.032 and p=0.0024, respectively) in percentage changes in IL-7 and MCP-1 in the control group and in the raloxifene group. Levels of low-density lipoprotein cholesterol (LDL-C) and E-selectin were decreased significantly in women who received raloxifene, but the percentage changes in LDL-C and E-selectin over a period of 12 months were not significantly correlated with percentage changes in IL-7 and MCP-1 over the same period. CONCLUSION: Circulating levels of IL-7 and MCP-1 decrease in postmenopausal women who received raloxifene.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.atherosclerosis.2008.09.014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18976766; ● Search Scopus @ Elsevier (PMID): 18976766; ● Search Scopus @ Elsevier (DOI): 10.1016/j.atherosclerosis.2008.09.014

(DOI: 10.1016/j.atherosclerosis.2008.09.014, PubMed: 18976766) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Riyo Kinouchi, Fumi Shimizu, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Neonatal immune challenge affects the regulation of estrus cyclicity and feeding behavior in female rats.,
International Journal of Developmental Neuroscience, Vol.27, No.1, 111-114, 2008.

(要約): A single immune challenge with lipopolysaccharide (LPS) in the neonatal period has a long-lasting influence on immune response. Using female Sprague-Dawley rats, we examined whether neonatal LPS challenge influences the life-long neuroendocrine sensitivity of reproductive function and feeding behavior to LPS, and whether stress-related neuropeptides and their receptors are involved in neonatal LPS-induced physiological change. On day 10 after birth, all pups were injected with LPS (100 microg/kg, i.p.) or saline. Then, in Experiment 1, LPS (100 microg/kg, i.p.) or saline was injected at diestrous in adulthood, and the length of the estrous cycle, 24h food intake and body weight change were recorded. In Experiment 2, the mRNA expression levels of corticotropin-releasing hormone (CRH), urocortin (UCN), urocortin 2 (UCN2), CRH receptor type 1 (CRH-R1) and CRH receptor type 2 (CRH-R2) in the hypothalamus were measured using real-time PCR. LPS injection in adulthood prolonged the estrous cycle in neonatal LPS-injected rats. LPS injection in adulthood decreased food intake and body weight in both neonatal LPS- and saline-injected rats, more so in the latter. Basal expressions of UCN2 and CRH-R2 mRNA were higher in neonatal LPS-injected rats than in saline-injected rats. These findings indicate that neonatal immune challenge influences the anti-stress regulation of the estrous cycle and feeding behavior in adulthood. Increased expression of UCN2 and CRH-R2 might enhance the sensitivity of the estrous cycle in suppressing the effects of LPS.
(キーワード): 加齢 (aging) / Animals / Animals, Newborn / Appetite Regulation / Corticotropin-Releasing Hormone / Estrous Cycle / Feeding Behavior / Female / Hypothalamo-Hypophyseal System / 視床下部 (hypothalamus) / Immune System Diseases / Inflammation Mediators / Lipopolysaccharides / RNA, Messenger / Rats / Rats, Sprague-Dawley / Receptors, Corticotropin-Releasing Hormone / Stress, Physiological / 時間 (time) / Up-Regulation / Urocortins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2008.10.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18996180; ● Search Scopus @ Elsevier (PMID): 18996180; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2008.10.003

(DOI: 10.1016/j.ijdevneu.2008.10.003, PubMed: 18996180) Toshiyuki Yasui, Hirokazu Uemura, Minoru Irahara, Makoto Arai, Noriko Kojimahara, Ryugo Okabe, Yasutomo Ishii, Shin-Ichi Tashiro and Hiroshi Sato :
Associations of endogenous sex hormones and sex hormone-binding globulin with lipid profiles in aged Japanese men and women.,
Clinica Chimica Acta, Vol.398, No.1-2, 43-47, 2008.

(要約): BACKGROUND: The associations of endogenous estrogen with lipid profiles and insulin resistance were conflicting in both men and women. We determined the associations of serum estradiol level and free estradiol index (FEI) with lipid profiles and insulin resistance in aged Japanese men and women. METHODS: One hundred seventy-four men and 208 postmenopausal women aged from 50 to 85 y were included in this study. Lipid profiles, fasting glucose and insulin as well as estradiol, testosterone, dehydroepiandrosterone-sulfate (DHEA-S) and sex hormone-binding globulin (SHBG) in serum were measured. RESULTS: Serum estradiol concentration in men showed a significant and negative correlation with high density lipoprotein-cholesterol (HDL-C) concentration and a significant and positive correlation with triglyceride (TG) after adjustment for age and body mass index (BMI). In addition, estradiol level was positively correlated with insulin and HOMA index. In women, serum levels of estradiol and testosterone were not correlated with lipid profiles and insulin resistance. SHBG concentration was negatively correlated with TG after adjustment for age and BMI in women. CONCLUSION: Endogenous estradiol level is associated with unfavorable lipid profiles and insulin resistance in aged men. In aged women, SHBG level is associated with favorable lipid profiles.
(キーワード): Aged / Aged, 80 and over / Androgens / Body Mass Index / Cholesterol, HDL / Dehydroepiandrosterone Sulfate / Estradiol / Female / Gonadal Steroid Hormones / Homeostasis / Humans / Insulin / Insulin Resistance / Japan / Lipids / Male / Middle Aged / Sex Hormone-Binding Globulin / Testosterone / Triglycerides
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cca.2008.08.006
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18755173; ● Search Scopus @ Elsevier (PMID): 18755173; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cca.2008.08.006

(DOI: 10.1016/j.cca.2008.08.006, PubMed: 18755173) Masahiro Murakami, Toshiya Matsuzaki, Takeshi Iwasa, Toshiyuki Yasui, Minoru Irahara, Tomohiro Osugi and Kazuyoshi Tsutsui :
Hypophysiotropic role of RFamide-related peptide-3 in the inhibition of LH secretion in female rats.,
The Journal of Endocrinology, Vol.199, No.1, 105-112, 2008.

(要約): Gonadotropin-inhibitory hormone (GnIH), a newly discovered hypothalamic RFamide peptide, inhibits reproductive activity by decreasing gonadotropin synthesis and release in birds. The gene of the mammalian RFamide-related peptides (RFRP) is orthologous to the GnIH gene. This Rfrp gene gives rise to the two biologically active peptides RFRP-1 (NPSF) and RFRP-3 (NPVF), and i.c.v. injections of RFRP-3 suppress LH secretion in several mammalian species. In this study, we show whether RFRP-3 affects LH secretion at the pituitary level and/or via the release of GnRH at the hypothalamus in mammals. To investigate the suppressive effects of RFRP-3 on the mean level of LH secretion and the frequency of pulsatile LH secretion in vivo, ovariectomized (OVX) mature rats were administered RFRP-3 using either i.c.v. or i.v. injections. Furthermore, the effect of RFRP-3 on LH secretion was also investigated using cultured female rat pituitary cells. With i.v. administrations, RFRP-3 significantly reduced plasma LH concentrations when compared with the physiological saline group. However, after i.c.v. RFRP-3 injections, neither the mean level of LH concentrations nor the frequency of the pulsatile LH secretion was affected. When using cultured pituitary cells, in the absence of GnRH, the suppressive effect of RFRP-3 on LH secretion was not clear, but when GnRH was present, RFRP-3 significantly suppressed LH secretion. These results suggest that RFRP-3 does not affect LH secretion via the release of GnRH, and that RFRP-3 directly acts upon the pituitary to suppress GnRH-stimulated LH secretion in female rats.
(キーワード): Animals / Cells, Cultured / Eating / Female / Gonadotropin-Releasing Hormone / Luteinizing Hormone / Male / Neuropeptides / Ovariectomy / 下垂体 (pituitary gland) / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1677/JOE-08-0197
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18653621; ● Search Scopus @ Elsevier (PMID): 18653621; ● Search Scopus @ Elsevier (DOI): 10.1677/JOE-08-0197

(DOI: 10.1677/JOE-08-0197, PubMed: 18653621) Yuka Miyatani, Toshiyuki Yasui, Hirokazu Uemura, Masayo Yamada, Toshiya Matsuzaki, Akira Kuwahara, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Associations of circulating adiponectin with estradiol and monocyte chemotactic protein-1 in postmenopausal women.,
Menopause, Vol.15, No.3, 536-541, 2008.

(要約): OBJECTIVE: The aim of the present study was to clarify the association of serum adiponectin concentrations with serum 17beta-estradiol concentrations in pre-, peri-, and postmenopausal women. In addition, the associations of serum adiponectin with serum concentrations of proinflammatory and anti-inflammatory cytokines were examined in women during the menopausal transition. DESIGN: A total of 197 women were enrolled in this study: 33 premenopausal women, 80 perimenopausal women, and 84 postmenopausal women. Serum adiponectin concentration was measured by an enzyme-linked immunosorbent assay. Serum concentrations of the proinflammatory cytokines interleukin (IL)-1beta, IL-6, and tumor necrosis factor alpha, anti-inflammatory cytokine IL-10, and the chemokines IL-8, macrophage inflammatory protein-1beta and monocyte chemotactic protein-1 were measured by using a multiplexed human cytokine assay. RESULTS: Serum adiponectin concentration showed a significant negative correlation with serum estradiol concentration (r= -0.400, P=0.001) in postmenopausal women but not in pre- and perimenopausal women, and this correlation was significant after adjustment for age and body mass index. Serum adiponectin concentration also showed a significant negative correlation with serum monocyte chemotactic protein-1 concentration (r= -0.244, P=0.05) in postmenopausal women. CONCLUSION: An increase in adiponectin level due to a decrease in estradiol results in a reduction in monocyte chemotactic protein-1 level in postmenopausal women, suggesting that adiponectin may be associated with a protective role against insulin resistance and atherosclerosis, which occur in the postmenopausal stage.
(キーワード): Adiponectin / Adult / Aged / Chemokine CCL2 / Cohort Studies / Cytokines / Estradiol / Female / Humans / Middle Aged / Postmenopause / Premenopause
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/gme.0b013e31815c85ed
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18347511; ● Search Scopus @ Elsevier (PMID): 18347511; ● Search Scopus @ Elsevier (DOI): 10.1097/gme.0b013e31815c85ed

(DOI: 10.1097/gme.0b013e31815c85ed, PubMed: 18347511) Fumi Shimizu, Toshiya Matsuzaki, Takeshi Iwasa, Naoko Tanaka, Masahiro Minakuchi, Akira Kuwahara, Toshiyuki Yasui, Hiroyuki Furumoto and Minoru Irahara :
Transition of leptin receptor expression during pubertal development in female rat pituitary.,
Endocrine Journal, Vol.55, No.1, 191-198, 2008.

(要約): Leptin is thought to play important roles in pubertal development and reproductive function in the female. Leptin receptor is expressed in various tissues including the pituitary and hypothalamus. We investigated the transition of leptin receptor (Ob-R) expression in female rat pituitary during pubertal development. Real-time RT-PCR was performed for long-form leptin receptor (Ob-Rb) and short-form leptin receptor (Ob-Ra) in the pituitary and cerebrum of the rats aged 4 (juvenile), 6 (pubertal), and 8 weeks (adult). Double immunohistochemical colocalization of Ob-R and luteinizing hormone (LH) was performed in pituitaries from 4-week-old female rats. Serum leptin concentrations of 4-, 6-, and 8-week-old rats were measured by radioimmunoassay. In the pituitary, expression of Ob-Rb mRNA in 4-week-old rats (1.00 +/- 0.16) was significantly higher than in 8-week-old rats (0.61 +/- 0.07, p<0.05), although expression of Ob-Ra mRNA did not differ among 4-, 6-, and 8-week-old rats. In cerebrum, Ob-Ra and Ob-Rb mRNA expressions did not differ significantly among 4-, 6-, and 8-week-old rats. Intense staining of Ob-R and colocalization of Ob-R and LH were seen in 4-week-old rat pituitary. On the other hand, serum concentrations of leptin in 6- and 8-week-old rats were significantly higher than those in 4-week-old rats (p<0.05, p<0.01, respectively). In conclusion, since the pituitary gonadotroph is a potential target of leptin, the juvenile rat pituitary might prepare for a subsequent increase of serum leptin concentration by expressing Ob-Rb.
(キーワード): Animals / Cells, Cultured / Cerebrum / Estradiol / Female / Follicle Stimulating Hormone / Gene Expression Regulation, Developmental / Leptin / 下垂体 (pituitary gland) / RNA, Messenger / Rats / Rats, Wistar / Receptors, Leptin / Sexual Maturation
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.K07E-029
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18250539; ● Search Scopus @ Elsevier (PMID): 18250539; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.K07E-029

(DOI: 10.1507/endocrj.K07E-029, PubMed: 18250539) Hirokazu Uemura, Toshiyuki Yasui, Y Miyatani, M Yamada, Mineyoshi Hiyoshi, Kokichi Arisawa and Minoru Irahara :
Circulating profiles of osteoprotegerin and soluble receptor activator of nuclear factor kappaB ligand in post-menopausal women.,
Journal of Endocrinological Investigation, Vol.31, No.2, 163-168, 2008.

(要約): OBJECTIVE: The aim of this study was to elucidate the detail profiles of circulating osteoprotegerin (OPG) and soluble receptor activator of nuclear factor kappaB ligand (sRANKL) in post-menopausal women. METHODS: Eighty Japanese post-menopausal women were enrolled in this cross-sectional study. Circulating OPG and free fraction of sRANKL (free sRANKL), PTH, calcium and phosphorus, age, years since menopause, body mass index, bone mineral density of the vertebral bodies (LBMD) and bone turnover markers were determined in each subject. RESULTS: In rank order correlation analysis, serum OPG concentrations had a significant positive correlation with age (r=0.291, p=0.024) and a marginal significant negative correlation with LBMD (r=-0.247, p=0.062). However they did not have correlations with LBMD or other parameters after adjustment for age. Serum free sRANKL concentrations had a significant positive correlation with age (r=0.332, p=0.010) and a significant negative correlation with LBMD (r=-0.608, p<0.001). This correlation with LBMD persisted after adjustment for age. In a multiple regression analysis with a stepwise model, the main determinants of LBMD were age and serum free sRANKL (p=0.015 and p=0.006, respectively). CONCLUSIONS: We found the increase in circulating OPG and sRANKL with age and a robust negative correlation between circulating free sRANKL and LBMD after adjustment for age. The increase in circulating free sRANKL may reflect directly or indirectly the conditions coexistent with bone loss in post-menopausal women.
(キーワード): Alkaline Phosphatase / Bone Density / Calcium / Collagen Type I / Female / Humans / Middle Aged / Osteoporosis, Postmenopausal / Osteoprotegerin / Parathyroid Hormone / Peptides / Phosphorus / Postmenopause / RANK Ligand / Solubility
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18362509; ● Search Scopus @ Elsevier (PMID): 18362509

(PubMed: 18362509) Toshiyuki Yasui, Hirokazu Uemura, Masayo Yamada, Toshiya Matsuzaki, Naoko Tsuchiya, Masamichi Noguchi, Mitsutoshi Yuzurihara, Yoshio Kase and Minoru Irahara :
Associations of interleukin-6 with interleukin-1beta, interleukin-8 and macrophage inflammatory protein-1beta in midlife women.,
Cytokine, Vol.41, No.3, 302-306, 2008.

(要約): OBJECTIVE: The aim of the present study was to determine the associations of interleukin (IL)-6 with other cytokines and chemokines and to compare these associations in peri- and postmenopausal women. METHODS: Ninety-nine perimenopausal and 92 postmenopausal women were enrolled in this study. Serum concentrations of IL-6, IL-1beta, IL-2, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, tumor necrosis factor (TNF)-alpha, interferon gamma, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage (GM)-CSF, macrophage inflammatory protein (MIP)-1beta and monocyte chemotactic protein (MCP)-1 were measured simultaneously using a multiplexed cytokine assay. RESULTS: Among the 17 cytokines, IL-6, IL-1beta, IL-5, IL-7, IL-8, IL-10, MCP-1 and MIP-1beta were detected in serum in more than 50% of the women. Serum levels of IL-4 and MCP-1 in postmenopausal women were significantly higher than those in perimenopausal women. Serum IL-6 concentrations showed significant and positive correlations with serum concentrations of IL-1beta, IL-8, MIP-1beta, IL-7 and MCP-1 in women regardless of menopausal status, and these correlations were still significant after adjustment for age and body mass index. CONCLUSION: Serum IL-6 concentration was found to be closely associated with serum concentrations of IL-1beta, IL-8, MIP-1beta, IL-7 and MCP-1 in women regardless of menopausal status, suggesting that these cytokines act in concert with the progression of several symptoms and various diseases.
(キーワード): Chemokine CCL4 / Cytokines / Female / Humans / Interleukin-1beta / Interleukin-6 / Interleukin-8 / Middle Aged / Perimenopause / Postmenopause
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cyto.2007.12.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18226916; ● Search Scopus @ Elsevier (PMID): 18226916; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cyto.2007.12.001

(DOI: 10.1016/j.cyto.2007.12.001, PubMed: 18226916) Sawako Kinouchi, Junichi Iga, Shu-ichi Ueno, Ken Yamauchi, Shusuke Numata, Hongwei Song, Satsuki Sumitani, Sumiko Shibuya-Tayoshi, Mari Haku, Toshiyuki Yasui, Minoru Irahara, Kyoko Morita, Kazuhito Rokutan and Tetsuro Ohmori :
FKBP5, SERT and COMT mRNA expressions in the peripheral leukocytes during menstruation cycle in healthy reproductive females.,
Neuroscience Letters, Vol.434, No.1, 124-128, 2008.

(要約): There have been several evidences that the mRNA expressions in the peripheral leukocytes may indicate not only physical but also psychological states. The purpose of this study is whether the mRNA expressional changes in the leukocytes are related to the mental states across the menstrual cycle in reproductive healthy female subjects. Thirty-eight female subjects (22.4+/-1.4 year-old) were participated in this study at three menstruation cycle periods (menstrual, follicular and luteal phase). The FKBP5 (FK506-binding protein gene), SERT (serotonin transporter gene) and COMT (catechol-o-methyltransferase gene) mRNA expressions in the leukocytes were determined with hormonal data. The psychological changes were assessed with self-rating hospital anxiety and depression scale (HADS). Only one thirds of subjects (n=12) had regular menstrual cycles during the experiment. So we analyzed the data from these 12 subjects. The anxiety score of each subject was changed across the menstrual cycle (Friedman test: P<0.05). The FKBP5 mRNA expression was significantly lower in the follicular phase than in the other phases but no changes were seen in either SERT or COMT mRNA expressions among the phases. In conclusion, there are differences of HADS anxiety score and FKBP5 mRNA expression in the leukocytes across the menstrual cycle but there is no correlation between anxiety scores and FKBP5 mRNA.
(キーワード): Adult / Anxiety / Anxiety Disorders / Biological Markers / Catechol O-Methyltransferase / Catecholamines / Depression / Depressive Disorder / 女性 (female) / Follicular Phase / Humans / Leukocytes / Luteal Phase / Menstrual Cycle / Premenstrual Syndrome / RNA, Messenger / Reference Values / Reproduction / Serotonin / Serotonin Plasma Membrane Transport Proteins / Tacrolimus Binding Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.neulet.2008.01.039
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18291582; ● Summary page in Scopus @ Elsevier: 2-s2.0-40649127657

(DOI: 10.1016/j.neulet.2008.01.039, PubMed: 18291582, Elsevier: Scopus) Hirokazu Uemura, Toshiyuki Yasui, Yuka Miyatani, Masayo Yamada, Mineyoshi Hiyoshi, Kokichi Arisawa and Minoru Irahara :
Circulating osteoprotegerin is associated with age and systolic blood pressure, but not with lipid profile or fasting glucose, in postmenopausal women.,
Menopause, Vol.15, No.1, 180-184, 2008.

(要約): OBJECTIVE: Osteoprotegerin (OPG), an inhibitor of osteoclastogenesis and osteoclast activation, has been reported to be linked to vascular biology. The aim of this study was to clarify the relationships between circulating OPG and the risk factors for vascular disorders in postmenopausal women. DESIGN: Eighty Japanese postmenopausal women were enrolled in this cross-sectional study. Clinical parameters (age, number of years since menopause, body mass index, systolic and diastolic blood pressure); serum concentrations of OPG, creatinine, calcium, and phosphorus; serum lipid profile; plasma glucose; and bone mineral density of the L2-4 vertebral bodies were determined for each woman. RESULTS: In rank-order correlation analysis, serum OPG concentrations had significant positive correlations with age (r = 0.29, P = 0.03), systolic blood pressure (r = 0.45, P < 0.01), diastolic blood pressure (r = 0.34, P < 0.01), and serum creatinine (r = 0.29, P = 0.04). Serum OPG concentration also had a marginally significant negative correlation with bone mineral density of the L2-4 vertebral bodies (r = -0.25, P = 0.06). However, serum OPG did not correlate with body mass index, serum lipid profile, or plasma glucose. The correlation of serum OPG with systolic blood pressure persisted after adjustment for both age and serum creatinine. CONCLUSIONS: These results suggest that increased circulating OPG in postmenopausal women is closely related to higher systolic blood pressure, which could cause atherosclerosis.
(キーワード): Age Factors / Atherosclerosis / Blood Glucose / Blood Pressure / Body Mass Index / Bone Density / Calcium / Creatinine / Cross-Sectional Studies / Female / Humans / Japan / Lipids / Middle Aged / Osteoprotegerin / Phosphorus / Postmenopause / Risk Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/gme.0b013e318046369b
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17545921; ● Search Scopus @ Elsevier (PMID): 17545921; ● Search Scopus @ Elsevier (DOI): 10.1097/gme.0b013e318046369b

(DOI: 10.1097/gme.0b013e318046369b, PubMed: 17545921) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Murakami, Fumi Shimizu, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Decreased expression of kisspeptin mediates acute immune/inflammatory stress-induced suppression of gonadotropin secretion in female rat.,
Journal of Endocrinological Investigation, Vol.31, No.7, 656-659, 2008.

(要約): Kisspeptin and its corresponding receptor, the G protein-coupled receptor 54, play an important role in reproductive systems. It has been suggested that reproductive disorders in metabolically disrupted animals are caused by the alteration of hypothalamic KiSS-1 systems. Immune/inflammatory challenge is also known to disrupt reproductive function. However, the effects of immune/inflammatory challenge on KiSS-1 systems have not been investigated. In this study, we showed that lipopolysaccharide (LPS) injection decreased hypothalamic KiSS-1 mRNA expression as well as plasma LH levels in ovariectomized rats. Indomethacin completely blocked the suppressive effects of LPS on LH secretion and KiSS-1 mRNA level. Furthermore, we showed that i.v. injection of kisspeptin increased plasma LH levels in LPS-administrated rats to the same degree as in saline-injected rats. These results suggest that KiSS-1 systems are sensitive to immune/inflammatory challenge conditions and transmit these signals into the central reproductive system.
(キーワード): Animals / Anti-Inflammatory Agents, Non-Steroidal / Female / Gonadotropin-Releasing Hormone / Hypothalamus / Indomethacin / Inflammation / Lipopolysaccharides / Luteinizing Hormone / Proteins / Rats / Rats, Wistar / Receptors, G-Protein-Coupled / Stress, Physiological
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18787387; ● Search Scopus @ Elsevier (PMID): 18787387

(PubMed: 18787387) Toshiyuki Yasui, Hirokazu Uemura, Minoru Irahara, Makoto Arai, Noriko Kojimahara, Ryugo Okabe, Ishii Yasutomo, Shin-ichi Tashiro and Hiroshi Sato :
Differences in sensitivity to cold in Japanese men and postmenopausal women aged > or =50 years.,
Gender Medicine, Vol.4, No.4, 359-366, 2007.

(要約): Sensitivity to cold is associated with several factors, such as aging, sex, and body composition. However, no previous studies have examined the differences in sensitivity to cold in men and women or the association of hormonal levels with sensitivity to cold. The aim of the present study was to clarify both the change in sensitivity to cold with aging and the difference in sensitivity to cold between men and women. Associations were also examined between circulating hormonal concentrations and the changes with aging and differences in sensitivity. This population-based cohort study enrolled healthy Japanese men and women aged > or = 50 years. A standardized 210-item health questionnaire was used to obtain information on symptoms of sensitivity to cold. Serum concentrations of luteinizing hormone, follicle-stimulating hormone (FSH), estradiol, testosterone, dehydroepiandrosterone sulfate, and sex hormone-binding globulin (SHBG) were measured. Of the 154 men and 180 women enrolled in this study, more women than men had sensitivity to cold. Whereas the percentage of men who had sensitivity to cold significantly increased with aging (P < 0.05), the percentage of women who had sensitivity to cold was already high (23.7%) at 50 to 60 years of age and did not change with aging. In men, advancing age and low body mass index (BMI) were significantly associated with sensitivity to cold (P < 0.05); however, age and BMI in women were not similarly associated. In addition, the effect of sex after adjustment for age was significant (P < 0.05), and there was also a numeric but nonsignificant effect of sex after adjustment for BMI. In men, low serum levels of the gonadal hormone FSH were significantly associated with sensitivity to cold in logistic analysis, but this association was nonsignificant after multivariate analysis. Serum concentrations of gonadal hormones and SHBG in women were not associated with sensitivity to col. The association of age with sensitivity to cold was different in men and women; the association of BMI with sensitivity to cold might be different in men and women. In addition, these changes in sensitivity to cold were not associated with circulating hormonal concentrations.
(キーワード): Age Factors / Aged / Aged, 80 and over / Aging / Asian Continental Ancestry Group / Body Mass Index / Cohort Studies / Cold Temperature / Female / Gonadal Steroid Hormones / Gonadotropins, Pituitary / Health Surveys / Humans / Male / Middle Aged / Sex Factors / Sex Hormone-Binding Globulin / Thermosensing
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S1550-8579(07)80065-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18215727; ● Search Scopus @ Elsevier (PMID): 18215727; ● Search Scopus @ Elsevier (DOI): 10.1016/S1550-8579(07)80065-9

(DOI: 10.1016/S1550-8579(07)80065-9, PubMed: 18215727) Fumi Shimizu, Toshiya Matsuzaki, Takeshi Iwasa, Masahiro Minakuchi, Akira Kuwahara, Toshiyuki Yasui and Minoru Irahara :
Estradiol suppresses NMU mRNA expression during sexual maturation in the female rat pituitary.,
International Journal of Developmental Neuroscience, Vol.26, No.3-4, 381-384, 2007.

(要約): Neuromedin U (NMU) suppresses food intake and gonadotropin secretion. However, the developmental transition of NMU expression in the pituitary gland and the regulation of NMU expression are unclear. The objective of this study was to examine the transition of the expression of NMU mRNA in the pituitary glands of female rats from the juvenile period to the mature period of development. Furthermore, factors such as estradiol, insulin, leptin, and inhibin A, whose expressions change throughout puberty and which affect gonadotropin secretion in pituitary cell culture, were examined. In the pituitary gland, the expression of NMU mRNA was significantly lower in 8-week-old rats than in 4- and 6-week-old rats. In the pituitary cell culture, the expressions of NMU mRNA in the estradiol- and insulin-treated groups were significantly lower than in the control group. These results suggest that the expression of NMU mRNA in the female rat pituitary is reduced as the rats develop from the pubertal to the mature period. Additionally, the reduction of NMU expression in the pituitary may be related to the increases in serum estradiol and insulin levels that occur during the pubertal period, which may negate NMU suppression of gonadotropin secretion.
(キーワード): Animals / Cells, Cultured / Estradiol / Female / Gonadotropins / Inhibins / Insulin / Leptin / Neuropeptides / Ovary / Pituitary Gland / RNA, Messenger / Rats / Rats, Wistar / Sexual Maturation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijdevneu.2007.11.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18258403; ● Search Scopus @ Elsevier (PMID): 18258403; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijdevneu.2007.11.002

(DOI: 10.1016/j.ijdevneu.2007.11.002, PubMed: 18258403) 上村浩一, 安井敏之, 宮谷友香, 山田正代, 日吉峰麗, 有澤孝吉, 苛原稔 :
閉経後婦人の血中osteoprotegerin濃度と心血管病変の危険因子との関係の検討,
日本更年期医学会雑誌, Vol.15, No.2, 233-238, 2007年.

(キーワード): osteoprotegerin / blood pressure / postmenopausal women
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1570572700958625920

(CiNii: 1570572700958625920) Toshiyuki Yasui, Junko Tomita, Yuka Miyatani, Masayo Yamada, Hirokazu Uemura, Minoru Irahara, Makoto Arai, Noriko Kojimahara, Ryugo Okabe, Yasutomo Ishii, Shin-ichi Tashiro and Hiroshi Sato :
Associations of adiponectin with sex hormone-binding globulin levels in aging male and female populations.,
Clinica Chimica Acta, Vol.386, No.1-2, 69-75, 2007.

(要約): Changes with aging in serum gonadal hormones, sex hormone-binding globulin (SHBG) and adiponectin, which are involved in insulin resistance and metabolic syndrome, are different in men and women. We examined the relationships of serum adiponectin concentrations with serum concentrations of hormones and SHBG in men and women. One hundred fifty-four men and 180 women aged from 50 to 85 years were included in the cross-sectional study. Serum concentrations of testosterone, estradiol, dehydroepiandrosterone-sulfate, SHBG and adiponectin were measured in men and women. Serum adiponectin concentration showed a significant positive correlation with serum SHBG concentration in men and women (r=0.404, p<0.0001 and r=0.348, p<0.0001, respectively). Multiple regression analysis also showed that SHBG was the significant predictor for adiponectin in men and women. Serum adiponectin concentration in men showed a significant positive correlation with total testosterone concentration, but that in women was not significantly correlated with total testosterone concentration. Both adiponectin and SHBG concentrations in men (r=-0.222, p=0.0051 and r=-0.334, p<0.0001, respectively) and women (r=-0.281, p=0.0014 and r=-0.251, p=0.0048, respectively) showed significant negative correlations with the homeostasis model assessment (HOMA) index. Correlations between adiponectin and SHBG were significantly positive in both men and women, and both adiponectin and SHBG might be involved in insulin resistance in both sexes.
(キーワード): Adiponectin / Aged / Aged, 80 and over / Aging / Body Mass Index / Female / 生体恒常性 (homeostasis) / Humans / インスリン抵抗性 (insulin resistance) / Male / Metabolic Syndrome X / Middle Aged / Models, Biological / Regression Analysis / Risk Assessment / Sex Factors / Sex Hormone-Binding Globulin / Testosterone
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cca.2007.08.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17825805; ● Search Scopus @ Elsevier (PMID): 17825805; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cca.2007.08.001

(DOI: 10.1016/j.cca.2007.08.001, PubMed: 17825805) 加地剛, 安井敏之, 須藤真功, 中山聡一朗, 森根幹生, 三谷龍史, 前田和寿, 苛原稔, 上村浩一 :
切迫早産管理が骨代謝に与える影響の検討(第1報),
Osteoporosis Japan, Vol.15, No.3, 448-451, 2007年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520573331027056896

(CiNii: 1520573331027056896) Kumiko Sakamoto, Yuki Hibino, Masaru Kihara, Shuji Yamano, Kazuhisa Maeda, Minoru Irahara and Aiko Yamauchi :
Prescribing patterns during pregnancy at Tokushima University Hospital,
Japanese Journal of Pharmaceutical Health Care and Sciences, Vol.33, No.4, 377-385, 2007.

(要約): The purpose of this study was to investigate the drugs prescribed for pregnant women at Tokushima University Hospital in Japan, and to evaluate the risks associated with the use of these drugs during pregnancy according to the pregnancy risk classification systems of the PDA, ADEC and that used in manufacturers' package inserts. Of 496 female outpatients who gave birth at Tokushima University Hospital, 81% had been prescribed at least one drug, and the mean was 4.0 drugs per patient. Analysis of the prescriptions revealed a pattern of drug use very different from that in other countries, in that Kampo (Japanese herbal) medicines were also prescribed. For more than half of the oral drugs prescribed, risks were not given in the PDA and/or ADEC pregnancy risk systems. In addition, there was no listing of pregnancy risks in the package inserts of 20 drugs. We therefore consider it to be very important to develop a globally harmonized system for evaluating the risks associated with prescription drugs taken during pregnancy.
(キーワード): pregnancy / medication / prescription patterns / risk classification
(出版サイトへのリンク): ● Publication site (DOI): 10.5649/jjphcs.33.377
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204775485440; ● Search Scopus @ Elsevier (DOI): 10.5649/jjphcs.33.377

(DOI: 10.5649/jjphcs.33.377, CiNii: 1390001204775485440) Toshiyuki Yasui, Masahiko Maegawa, Junko Tomita, Yuka Miyatani, Masayo Yamada, Hirokazu Uemura, Shu-ichi Ueno, Shusuke Numata, Tetsuro Ohmori, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Shuichi Takeda and Minoru Irahara :
Association of serum cytokine concentrations with psychological symptoms in midlife women.,
Journal of Reproductive Immunology, Vol.75, No.1, 56-62, 2007.

(要約): The purpose of the present study was to clarify the association of serum cytokine concentrations, determined using a multiplexed cytokine assay, with psychological symptoms in midlife women. Fifty-three peri- and post-menopausal women with and without psychological symptoms in Greene's climacteric scale were enrolled in this study. Levels of 17 cytokines in serum samples were measured simultaneously using a multiplexed human cytokine assay. Serum interleukin (IL)-6 concentration in women with psychological symptoms (2.71+/-047 pg/ml) was significantly (p=0.009) higher than that in women without psychological symptoms (0.98+/-0.18 pg/ml). Serum IL-8 concentration in women with psychological symptoms (33.4+/-8.17 pg/ml) was also significantly (p=0.022) higher than that in women without psychological symptoms (7.87+/-1.64 pg/ml). In addition, serum IL-10 concentration in women with psychological symptoms (0.74+/-0.26 pg/ml) was significantly (p=0.048) higher than that in women without psychological symptoms (0.07+/-0.04 pg/ml). Tumor necrosis factor (TNF)-alpha in serum was detected only in women with psychological symptoms. Serum IL-2 concentration in women with psychological symptoms tended (p=0.066) to be higher than that in women without psychological symptoms. No significant differences were found between levels of other cytokines in women with and without psychological symptoms. Psychological stress manifested as climacteric symptoms in midlife women may be associated with increases in serum concentrations of IL-6, IL-8, IL-10, and TNF-alpha.
(キーワード): Cytokines / Female / Humans / Menopause / Middle Aged / Perimenopause / Postmenopause / Stress, Psychological
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jri.2007.02.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17412426; ● Search Scopus @ Elsevier (PMID): 17412426; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jri.2007.02.004

(DOI: 10.1016/j.jri.2007.02.004, PubMed: 17412426) Takeshi Iwasa, Toshiya Matsuzaki, Masahiro Minakuchi, Naoko Tanaka, Fumi Shimizu, Yohko Hirata, Akira Kuwahara, Toshiyuki Yasui, Masahiko Maegawa and Minoru Irahara :
Diagnostic performance of serum total testosterone for Japanese patients with polycystic ovary syndrome.,
Endocrine Journal, Vol.54, No.2, 233-238, 2007.

(要約): It is reported that the incidence of clinical and biochemical hyperandrogenism may be lower in Japanese patients with PCOS. Hyperandrogenism is included as a referential but not as an essential factor in the diagnostic criteria of the Japanese Society of Obstetrics and Gynecology (JSOG 1993). However, some patients with the typical clinical features of PCOS are not diagnosed with PCOS using JSOG 1993 criteria because they do not have a high LH level, which is defined as essential for diagnosis. In this study, we compared total testosterone (T) levels between Japanese patients with PCOS diagnosed using the JSOG 1993 criteria and normal menstrual women (controls). Fifty controls and 46 patients with PCOS were enrolled in this study. Furthermore, we evaluated the sensitivity and specificity of each cut-off value of T. The mean T level of patients with PCOS was significantly higher than that of the control (86 +/- 48 vs 68 +/- 46, P<0.01), and the prevalence rates of hyperandrogenism (T >114 ng/dL; defined as the mean +2SD of the control) were 10.2% in patients with PCOS and 4% in controls. The area under the ROC curve of T was 0.72, and there was no decision threshold to diagnose PCOS by T alone with both high sensitivity and high specificity. If the threshold is set as 110 ng/dL in order to gain high specificity, 94% of women whose serum level passed the threshold will be patients with PCOS. Although T should not be used as an independent essential factor of Japanese PCOS, it might be useful as a complementary factor in order to diagnose patients who have typical clinical features of PCOS but does not fulfill the JSOG 1993 criteria for PCOS.
(キーワード): Asian Continental Ancestry Group / Chemiluminescent Measurements / Female / Humans / Hyperandrogenism / Immunoassay / Polycystic Ovary Syndrome / Prevalence / ROC Curve / Sensitivity and Specificity / Testosterone
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.K06-159
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17283381; ● Search Scopus @ Elsevier (PMID): 17283381; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.K06-159

(DOI: 10.1507/endocrj.K06-159, PubMed: 17283381) Takashi Kaji, Toshiyuki Yasui, Masanori Suto, Ryuji Mitani, Mikio Morine, Hirokazu Uemura, Kazuhisa Maeda and Minoru Irahara :
Effect of bed rest during pregnancy on bone turnover markers in pregnant and postpartum women.,
Bone, Vol.40, No.4, 1088-1094, 2007.

(要約): The aims of our study were to evaluate the changes in bone turnover markers during pregnancy and puerperium as a longitudinal study and to elucidate the effect of bed rest during pregnancy on bone turnover markers in pregnant and postpartum women. The study population comprised 27 Japanese pregnant women aged 23-40 years. All women were recruited for the longitudinal study from the outpatients clinic of the Department of Obstetrics and Gynecology, Tokushima University Hospital. Concentrations of serum bone-specific alkaline phosphatase (BAP), urinary cross-linked type I collagen N-telopeptides (NTx), serum NTx and urinary C-terminal telopeptide of type I collagen (CTx) were measured at 10, 26, 30 and 36 weeks of pregnancy and at 4 days and 1 month postpartum. In addition, we recruited 15 pregnant women (aged 25-35 years) who were treated by bed rest before 30 weeks of pregnancy for threatened premature delivery and compared bone turnover markers in these women with those in 22 normal pregnant women (aged 22-39 years). Concentrations of serum BAP, serum NTx, urinary NTx and urinary CTx were measured at 30 and 34 weeks of pregnancy and at 4 days and 1 month postpartum. In the longitudinal study, serum BAP concentration at 1 month postpartum was significantly higher than that at any stage of pregnancy and that at 4 days postpartum. Urinary concentration of NTx increased gradually during pregnancy and showed a peak at 36 weeks of pregnancy, followed by a decrease in the postpartum period. Serum NTx concentration and urinary CTx concentration showed the same patterns of change as that of urinary NTx concentration. In the comparison study, urinary concentrations of NTx and CTx at 30 and 34 weeks of pregnancy in women with bed rest were significantly (p<0.0001 and p<0.001, respectively) higher than those in normal pregnant women. Serum NTx concentration at 34 weeks of pregnancy in women with bed rest was also significantly (p=0.0029) higher than that in normal pregnant women. Serum BAP concentration at 34 weeks of pregnancy in women with bed rest was significantly (p=0.0038) higher than that in normal pregnant women, and these high levels were maintained during puerperium. Serum BAP concentration at 34 weeks of pregnancy was significantly correlated with duration of bed rest (r=0.767, p=0.0041). Immobilization due to bed rest during pregnancy is associated with increases in bone turnover markers in pregnant and postpartum women. Concentrations of bone resorption markers increased rapidly at the start of bed rest, while the concentration of a bone formation marker gradually increased toward puerperium.
(キーワード): Adult / Alkaline Phosphatase / Bed Rest / Biological Markers / Bone Remodeling / Calcium / Case-Control Studies / Collagen Type I / Female / Humans / Longitudinal Studies / Peptides / Postpartum Period / Pregnancy / Pregnancy Complications / Prospective Studies
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bone.2006.11.018
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17229597; ● Search Scopus @ Elsevier (PMID): 17229597; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bone.2006.11.018

(DOI: 10.1016/j.bone.2006.11.018, PubMed: 17229597) Takeshi Iwasa, Toshiya Matsuzaki, Machiko Kiyokawa, Fumi Shimizu, Masahiro Minakuchi, Akira Kuwahara, Masahiko Maegawa, Toshiyuki Yasui and Minoru Irahara :
The type 2 corticotrophin-releasing hormone receptor mediates orexin A-induced luteinising hormone suppression in ovariectomised rats.,
Journal of Neuroendocrinology, Vol.19, No.9, 732-738, 2007.

(要約): Orexins are thought to be regulatory factors of the arousal and sleep patterns. They also affect immune, feeding, autonomic and neuroendocrine systems. We have previously shown that intracerebroventricular (i.c.v.) injection of orexin decreases pulsatile luteinising hormone (LH) secretion in ovariectomised (OVX) rats. However, the details of this mechanism have not been fully examined. Intracerebroventricular injection of orexin A also stimulates corticotrophin-releasing hormone (CRH) systems, which have been implicated in the stress-induced suppression of reproductive function. In the present study, we investigated the role of CRH systems in orexin-induced LH suppression. OVX rats were implanted with i.c.v. and intravenous (i.v.) cannulae. After i.c.v. injection of orexin and/or CRH receptor antagonists, blood samples were collected through the i.v. cannula at 6-min intervals for 120 min for LH measurement. Intracerebroventricular injection of orexin A or B (3 nmol/2.5 microl) suppressed pulsatile LH secretion. Coadministration of orexin A and alpha-helical corticotrophic-releasing factor (CRF), a nonselective CRH receptor antagonist (13 nmol/2.5 microl), or astressin(2)B, a selective type2 (CRH-R2) CRH receptor antagonist (28 nmol/2.5 microl), partly restored pulsatile LH secretion. Orexin B-induced LH suppression was not restored by alpha-helical CRF. In addition, i.c.v. injection of orexin A increased CRH and urocortin II (UcnII), but not Ucn mRNA levels, in the hypothalamus. These findings suggest that CRH-R2 mediates orexin A-induced LH suppression and it is possible that CRH and UcnII in the hypothalamus are involved in this pathway.
(キーワード): Animals / Corticotropin-Releasing Hormone / Female / 視床下部 (hypothalamus) / Intracellular Signaling Peptides and Proteins / Luteinizing Hormone / Neuropeptides / Ovariectomy / Rats / Rats, Wistar / Receptors, Corticotropin-Releasing Hormone / Urocortins
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2826.2007.01583.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17680889; ● Search Scopus @ Elsevier (PMID): 17680889; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2826.2007.01583.x

(DOI: 10.1111/j.1365-2826.2007.01583.x, PubMed: 17680889) Toshiyuki Yasui, Masahiko Maegawa, Junko Tomita, Yuka Miyatani, Masayo Yamada, Hirokazu Uemura, Toshiya Matsuzaki, Akira Kuwahara, Masaharu Kamada, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Shuichi Takeda and Minoru Irahara :
Changes in serum cytokine concentrations during the menopausal transition.,
Maturitas, Vol.56, No.4, 396-403, 2006.

(要約): The purpose of the present study was to clarify the changes in serum concentrations of 17 cytokines in healthy women during the menopausal transition by using a multiplexed cytokine assay and to clarify the associations of these cytokines with serum estradiol concentration. Sixteen premenopausal, 54 perimenopausal and 52 postmenopausal women were enrolled in this study. Seventeen cytokines in serum samples were measured simultaneously using a Bio-Plex human cytokine 17-Plex assay. Serum IL-6 concentration showed a weak positive correlation with age (r=0.196, p<0.05). Postmenopausal women for whom less than 5 years had passed since menopause showed significant (p<0.05) increase in serum concentrations of IL-2, GM-CSF and G-CSF, while serum IL-4 concentration was significantly (p<0.05) increased in postmenopausal women for whom more than 5 years had passed since menopause. Serum estradiol concentration showed a significant negative correlation with serum IL-6 concentration and weak negative correlations with serum concentrations of IL-2, IL-8 and GM-CSF. We were able to simultaneously measure the levels of 17 cytokines using a highly sensitive cytokine assay, and we found that the changes in serum cytokine concentrations during the menopausal transition differed. We also found that serum IL-6 concentration during the menopausal transition was negatively correlated with serum estradiol concentration.
(キーワード): Adult / Aged / 細胞質分裂 (cytokinesis) / Estradiol / Female / Granulocyte Colony-Stimulating Factor / Granulocyte-Macrophage Colony-Stimulating Factor / Humans / Interleukin-2 / Interleukin-4 / Interleukin-6 / Menopause / Middle Aged
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.maturitas.2006.11.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17164077; ● Search Scopus @ Elsevier (PMID): 17164077; ● Search Scopus @ Elsevier (DOI): 10.1016/j.maturitas.2006.11.002

(DOI: 10.1016/j.maturitas.2006.11.002, PubMed: 17164077) Toshiyuki Yasui, Hirokazu Uemura, J Tomita, Y Miyatani, M Yamada, M Miura and Minoru Irahara :
Association of serum undercarboxylated osteocalcin with serum estradiol in pre-, peri- and early post-menopausal women.,
Journal of Endocrinological Investigation, Vol.29, No.10, 913-918, 2006.

(要約): We investigated changes in serum concentration of undercarboxylated osteocalcin (ucOC), which is a sensitive marker of vitamin K status, and association of ucOC concentration with estradiol concentration in pre-, peri- and early post-menopausal women. The study population consisted of 193 pre-, peri- and post-menopausal Japanese women aged 39-66 yr. Serum ucOC concentration was measured to assess vitamin K status; serum concentrations of intact osteocalcin (OC) and bone-specific alkaline phosphatase (BAP) were measured as bone formation markers; and urine concentration of N-telopeptide was measured as a bone resorption marker. Serum estradiol and estrone concentrations were measured by a highly sensitive radioimmunoassay. Bone mineral density (BMD) was measured at the lumbar spine. Serum concentration of ucOC in peri-menopausal women was significantly (p=0.0005) higher than that in pre-menopausal women, while serum OC concentration in post-menopausal women for whom 1 yr had passed since menopause was significantly (p=0.0003, p=0.024, respectively) higher than the concentrations in pre-menopausal and peri-menopausal women. Serum ucOC concentration showed a significant negative correlation with estradiol concentration (r=-0.372, p<0.0001) and a significant positive correlation with serum FSH concentration (r=0.324, p<0.0001). Serum OC concentration was positively correlated with serum FSH concentration (r=0.317, p<0.001). The results showed that the change in ucOC concentration during the menopausal transition is different from that in OC concentration. In addition, serum ucOC concentration is closely associated not only with FSH concentration but also estradiol concentration.
(キーワード): Adult / Aged / Aging / Alkaline Phosphatase / Bone Density / Collagen Type I / Cross-Sectional Studies / Estradiol / Female / Follicle Stimulating Hormone / Humans / Lumbar Vertebrae / Middle Aged / Osteocalcin / Peptides / Perimenopause / Postmenopause / Premenopause / Vitamin K
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17185901; ● Search Scopus @ Elsevier (PMID): 17185901

(PubMed: 17185901) Toshiyuki Yasui, Hirokazu Uemura, Junko Tomita, Yuka Miyatani, Masayo Yamada, Akira Kuwahara, Toshiya Matsuzaki, Masahiko Maegawa, Masakazu Miura and Minoru Irahara :
Change in serum undercarboxylated osteocalcin concentration in bilaterally oophorectomized women.,
Maturitas, Vol.56, No.3, 288-296, 2006.

(要約): We investigated changes in serum undercarboxylated osteocalcin (ucOC) concentrations, bone turnover markers and spine bone mineral density (BMD) in women who had undergone bilateral oophorectomy during the premenopausal period. The study population comprised 141 bilaterally oophorectomized and 32 premenopausal women for a cross-sectional study. The longitudinal study consisted of 21 bilaterally oophorectomized women. Serum ucOC concentration, serum concentrations of intact osteocalcin (OC) and bone-specific alkaline phosphatase (BAP) as bone formation markers, urine N-telopeptide (NTx) concentration as a bone resorption marker and serum parathyroid hormone (PTH) concentration were measured. Serum concentration of ucOC in women at 1 month after bilateral oophorectomy was significantly (p<0.05) higher than that in premenopausal women and the high level was sustained after surgical menopause. On the other hand, serum OC concentration at 1 month after surgical menopause was not different from that in premenopausal women. In the longitudinal study, serum ucOC concentration at 1 month after surgical menopause was significantly (p<0.05) increased compared to that before bilateral oophorectomy, while serum OC concentrations before and at 1 month after surgical menopause were not significantly different. The results of this study showed that serum ucOC concentration rapidly increases in women after bilateral oophorectomy and that change in serum ucOC concentration after surgical menopause is different from change in serum OC concentration.
(キーワード): Adult / Alkaline Phosphatase / Biological Markers / Bone Density / Bone and Bones / 二酸化炭素 (carbon dioxide) / Cross-Sectional Studies / Female / Humans / Longitudinal Studies / Middle Aged / Osteocalcin / Ovariectomy / Parathyroid Hormone / Postmenopause / Premenopause
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.maturitas.2006.09.002
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17030103; ● Search Scopus @ Elsevier (PMID): 17030103; ● Search Scopus @ Elsevier (DOI): 10.1016/j.maturitas.2006.09.002

(DOI: 10.1016/j.maturitas.2006.09.002, PubMed: 17030103) Toshiyuki Yasui, Hirokazu Uemura, Junko Tomita, Yuka Miyatani, Masayo Yamada, Akira Kuwahara, Toshiya Matsuzaki, Masahiko Maegawa, Naoko Tsuchiya, Mitsutoshi Yuzurihara, Shuichi Takeda and Minoru Irahara :
Association of interleukin-8 with hot flashes in premenopausal, perimenopausal, and postmenopausal women and bilateral oophorectomized women.,
The Journal of Clinical Endocrinology and Metabolism, Vol.91, No.12, 4805-4808, 2006.

(要約): The purpose of this study was to identify serum cytokine concentrations in premenopausal, perimenopausal, and postmenopausal women and bilateral oophorectomized women with hot flashes. Serum concentrations of 17 cytokines were simultaneously measured using a multiplexed human cytokine assay in 129 premenopausal, perimenopausal, and postmenopausal women and 50 bilateral oophorectomized women. Serum IL-8 concentrations in midlife women and bilateral oophorectomized women with severe hot flashes were significantly higher than the concentrations in women without hot flashes and women with mild and moderate hot flashes. Serum macrophage inflammatory protein-1beta concentration in women with severe hot flashes was significantly higher than those in women without hot flashes and women with mild and moderate hot flashes. Serum IL-8 concentrations in premenopausal, perimenopausal, and postmenopausal women and bilateral oophorectomized women with hot flashes were significantly higher than those in women without hot flashes. IL-8 may be associated with peripheral vasodilation in women with hot flashes.
(キーワード): Adult / Chemokine CCL4 / Cytokines / Female / Hot Flashes / Humans / Interleukin-6 / Interleukin-8 / Macrophage Inflammatory Proteins / Middle Aged / Ovariectomy / Perimenopause / Postmenopause / Premenopause
(出版サイトへのリンク): ● Publication site (DOI): 10.1210/jc.2006-1100
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17018658; ● Search Scopus @ Elsevier (PMID): 17018658; ● Search Scopus @ Elsevier (DOI): 10.1210/jc.2006-1100

(DOI: 10.1210/jc.2006-1100, PubMed: 17018658) Junko Komatsu, Shuji Yamano, Akira Kuwahara, Akira Tokumura and Minoru Irahara :
The signalling pathways linking to lysophosphatidic acid-promoted meiotic maturation,
Life Sciences, Vol.79, No.5, 506-511, 2006.

(要約): The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice were studied. When mouse oocyte-cumulus cells complexes were cultured with 10(-5) M lysophosphatidic acid (the LPA group), the rate of oocyte nuclear maturation was significantly increased. Additions of pertussis toxin, genistein, U73122, Ro320432, PD98059 or SB203580 significantly suppressed the increase in lysophosphatidic acid-stimulated nuclear maturation rate. These results suggested that Gi/o-coupled lysophosphatidic acid receptors activate phosphatidylinositol-specific phospholipase C, and result in ERK and MAP kinase activation, which is triggered by diacylglycerol-dependent protein kinase C. When intracellular cAMP concentrations of oocytes in the LPA and control groups were measured using the acetylation assay, the intracellular cAMP concentration of an oocyte in the LPA group was significantly lower than the control oocyte (0.117+/-0.04 fmol/oocyte vs. 0.176+/-0.036 fmol/oocyte, p<0.05). In conclusion, our results suggested that lysophosphatidic acid stimulates phospholipase C through a Gi-protein linked receptor on the surface of mouse cumulus cells and stimulates both extracellular signal-regulated kinase and p38 mitogen-activated kinase, resulting in the closure or loose of gap junctions between cumulus cells and the oocyte. The resultant early decrease of oocyte cAMP levels may promote nuclear maturation of mouse oocytes in vitro.
(キーワード): Animals / Cyclic AMP / Enzyme Inhibitors / Female / Genistein / Lysophospholipids / Meiosis / Mice / Oocytes / Pertussis Toxin / Signal Transduction
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2006.01.028
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16492384; ● Search Scopus @ Elsevier (PMID): 16492384; ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2006.01.028

(DOI: 10.1016/j.lfs.2006.01.028, PubMed: 16492384) Toshiyuki Yasui, Yuka Miyatani, Junko Tomita, Masayo Yamada, Hirokazu Uemura, Masakazu Miura and Minoru Irahara :
Effect of vitamin K2 treatment on carboxylation of osteocalcin in early postmenopausal women.,
Gynecological Endocrinology, Vol.22, No.8, 455-459, 2006.

(要約): We examined the serum level of undercarboxylated osteocalcin (uc OC), which is a sensitive marker of vitamin K status, and levels of bone turnover markers in early postmenopausal women receiving vitamin K2 treatment with or without vitamin D3. Thirty-four postmenopausal women with a mean age of 53 years whose bone mineral density (BMD) was less than 0.809 g/cm2 (osteopenia and osteoporosis) were treated with vitamin K2 or with a combination of vitamin K2 and vitamin D3. Seventeen women received daily oral administration of 45 mg vitamin K2 and 17 women received daily oral administration of 45 mg vitamin K2 plus 0.75 microg 1alpha-hydroxyvitamin D3. Serum levels of uc OC, intact osteocalcin (OC) and bone alkaline phosphatase (BAP), urinary deoxypyridinoline (DPD) levels and BMD at the lumbar spine were measured before and at 1 and 2 years after the start of treatment. Serum uc OC levels in women treated with vitamin K2 alone and with both vitamin K2 and vitamin D3 decreased significantly (p < 0.05). Serum levels of intact OC and BAP in women treated with vitamin K2 did not show significant changes, while those in women who received the combined treatment decreased significantly (p < 0.05). On the other hand, urinary DPD level in women treated with vitamin K2 did not change, while that in women who received the combined treatment tended to decrease (p < 0.1). Serum uc OC levels in early postmenopausal women who received vitamin K2 decreased due to carboxylation of uc OC. Combined treatment with vitamin K2 and vitamin D3 may be effective for sustaining BMD in early postmenopausal women whose bone turnovers are highly activated.
(キーワード): Alkaline Phosphatase / Amino Acids / Bone Density / Bone Density Conservation Agents / Bone Diseases, Metabolic / Cholecalciferol / Drug Therapy, Combination / Female / Humans / Middle Aged / Osteocalcin / Osteoporosis, Postmenopausal / Postmenopause / Vitamin K 2
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09513590600900402
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17012108; ● Search Scopus @ Elsevier (PMID): 17012108; ● Search Scopus @ Elsevier (DOI): 10.1080/09513590600900402

(DOI: 10.1080/09513590600900402, PubMed: 17012108) Kumiko Sakamoto, Yuki Hibino, Shuji Yamano, Kazuhisa Maeda, Minoru Irahara and Aiko Yamauchi :
Prescription drug use during pregnancy in Japan,
Reproductive Toxicology, Vol.22, No.2, 283, 2006. Toshiyuki Yasui, Hirokazu Uemura, Junko Tomita, Yuka Umino, Masayo Yamada, Akira Kuwahara, Toshiya Matsuzaki, Masahiko Maegawa, Masakazu Miura and Minoru Irahara :
Different effects of oral conjugated equine estrogens and transdermal estrogen on undercarboxylated osteocalcin concentration in postmenopausal women.,
Menopause, Vol.13, No.4, 651-659, 2006.

(要約): Undercarboxylated osteocalcin (ucOC) is a sensitive marker of vitamin K status, and triglyceride (TG) has been shown to be the main transporter of vitamin K. In the present study, we examined the difference between ucOC concentrations in postmenopausal women receiving hormone therapy (HT) with oral conjugated equine estrogens (CEE) and transdermal estradiol (TE2). We also examined the associations of ucOC concentration with estradiol concentration and TG. Ninety-two postmenopausal women were recruited for this study. Serum concentrations of ucOC, intact osteocalcin, estradiol, and TG were measured before and after 12 months of HT. Forty-six women received oral administration of 0.625 mg of CEE and 2.5 mg of medroxyprogesterone acetate daily, and 46 women received transdermal administration of 50 mug of 17beta-estradiol twice weekly and 2.5 mg of medroxyprogesterone acetate daily. The ucOC concentration in women during HT with oral CEE was significantly (P < 0.01) lower than that in women during HT with TE2. Serum estradiol concentrations during HT with CEE showed a significant inverse correlation with ucOC concentrations and the ratio of ucOC/OC during HT (P < 0.05 and P < 0.01, respectively). In addition, the serum ucOC concentration in women with an increased percentage of change in TG was significantly (P < 0.01) lower than that in women with a decreased percentage of change in TG during HT with oral CEE. The effect of HT with TE2 on ucOC concentration in women is weaker than the effect of HT with oral CEE. Suppression of ucOC concentration in postmenopausal women during HT with oral CEE might be associated with the effect of vitamin K through increased TG induced by oral CEE.
(キーワード): Administration, Cutaneous / Administration, Oral / Alkaline Phosphatase / Amino Acids / Bone Density / Estradiol / Estrogen Replacement Therapy / Estrogens, Conjugated (USP) / Female / Humans / Middle Aged / Osteocalcin / Osteoporosis, Postmenopausal / Postmenopause / Treatment Outcome / Triglycerides
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/01.gme.0000227334.50867.80
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16837887; ● Search Scopus @ Elsevier (PMID): 16837887; ● Search Scopus @ Elsevier (DOI): 10.1097/01.gme.0000227334.50867.80

(DOI: 10.1097/01.gme.0000227334.50867.80, PubMed: 16837887) 上村浩一, 安井敏之, 海野友香, 冨田純子, 山田正代, 苛原稔 :
塩酸ラロキシフェンが骨・脂質代謝と内分泌系に及ぼす作用の検討,
Osteoporosis Japan, Vol.14, No.3, 479-483, 2006年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523669554703857024

(CiNii: 1523669554703857024) 山内あい子, 坂本久美子, 日比野有紀, 中馬寛, 苛原稔 :
妊娠期の薬物療法∼その実態と母児への影響∼,
性差と医療, Vol.3, No.5, 27(521)-32(526), 2006年. Dan Kinoshita, Fumiko Hirota, Tsuneyasu Kaisho, Michiyuki Kasai, Keisuke Izumi, Yoshimi Bando, Yasuhiro Mouri, Akemi Matsushima, Shino Niki, Hongwei Han, Kiyotaka Oshikawa, Noriyuki Kuroda, Masahiko Maegawa, Minoru Irahara, Kiyoshi Takeda, Shizuo Akira and Mitsuru Matsumoto :
Essential role of IkappaB kinase alpha in thymic organogenesis required for the establishment of self-tolerance.,
The Journal of Immunology, Vol.176, No.7, 3995-4002, 2006.

(要約): IkappaB kinase (IKK) alpha exhibits diverse biological activities through protein kinase-dependent and -independent functions, the former mediated predominantly through a noncanonical NF-kappaB activation pathway. The in vivo function of IKKalpha, however, still remains elusive. Because a natural strain of mice with mutant NF-kappaB-inducing kinase (NIK) manifests autoimmunity as a result of disorganized thymic structure with abnormal expression of Rel proteins in the thymic stroma, we speculated that the NIK-IKKalpha axis might constitute an essential step in the thymic organogenesis that is required for the establishment of self-tolerance. An autoimmune disease phenotype was induced in athymic nude mice by grafting embryonic thymus from IKKalpha-deficient mice. The thymic microenvironment that caused autoimmunity in an IKKalpha-dependent manner was associated with defective processing of NF-kappaB2, resulting in the impaired development of thymic epithelial cells. Thus, our results demonstrate a novel function for IKKalpha in thymic organogenesis for the establishment of central tolerance that depends on its protein kinase activity in cooperation with NIK.
(キーワード): Animals / Gene Expression Regulation / I-kappa B Kinase / Mice / ノックアウトマウス (knockout mice) / Mutation / NF-kappa B / Oncogene Proteins v-rel / Organogenesis / Protein-Serine-Threonine Kinases / Self Tolerance / Thymus Gland
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16547234; ● Summary page in Scopus @ Elsevier: 2-s2.0-33646055527

(PubMed: 16547234, Elsevier: Scopus) 安井敏之, 宮谷友香, 冨田純子, 山田正代, 上村浩一, 苛原稔, 土屋直子, 譲原光利, 竹田秀一 :
更年期障害に対する漢方治療中のサイトカインの動態. 23, 76-79, 2006.04.,
産婦人科漢方研究のあゆみ, Vol.23, No.., 76-79, 2006年. Toshiyuki Yasui, Hirokazu Uemura, Yuka Umino, Masayo Yamada, Akira Kuwahara, Toshiya Matsuzaki, Masahiko Maegawa, Hiroyuki Furumoto, Masakazu Miura and Minoru Irahara :
Undercarboxylated osteocalcin concentration in postmenopausal women receiving hormone therapy daily and on alternate days.,
Menopause, Vol.13, No.2, 314-322, 2006.

(要約): Undercarboxylated osteocalcin (ucOC) is a sensitive marker of vitamin K status. The authors examined the difference in serum ucOC concentrations in postmenopausal women receiving hormone therapy (HT) daily and on alternate days, and assessed the association between ucOC and triglyceride concentrations, which are related to the transport of vitamin K. Seventy-three postmenopausal women were recruited for this study. Thirty-seven women received 0.625 mg of conjugated equine estrogens (CEE) and 2.5 mg of medroxyprogesterone acetate (MPA) daily, and 36 women received 0.625 mg of CEE and 2.5 mg of MPA on alternate days. The concentrations of serum ucOC, bone turnover markers, lipid profiles, and hormones were measured before and after 12 months of HT. The ucOC concentration in women taking HT daily was significantly (P < 0.01) lower than that in women taking HT on alternate days. Serum ucOC concentrations during HT showed a significant (P < 0.01) inverse correlation with estradiol concentrations during HT. Serum estradiol concentrations during HT showed a significant (P < 0.01) positive correlation with triglyceride concentrations during HT. Furthermore, ucOC concentrations during HT showed a significant (P < 0.05) inverse correlation with triglyceride concentrations in women receiving HT. The effect of HT on alternate days on ucOC concentration was weaker than the effect of HT daily. In addition, ucOC concentration after 12 months of HT daily might be decreased due to the conversion of ucOC to carboxylated OC by the effect of vitamin K through increased triglyceride levels induced by oral CEE.
(キーワード): Biological Markers / Bone Remodeling / 二酸化炭素 (carbon dioxide) / Drug Administration Schedule / Estradiol / Estrogen Replacement Therapy / Estrogens, Conjugated (USP) / Estrone / Female / Follow-Up Studies / Humans / Lipids / Medroxyprogesterone Acetate / Middle Aged / Osteocalcin / Postmenopause / Triglycerides / Vitamin K
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/01.gme.0000177908.40257.cf
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16645545; ● Summary page in Scopus @ Elsevier: 2-s2.0-33646809401

(DOI: 10.1097/01.gme.0000177908.40257.cf, PubMed: 16645545, Elsevier: Scopus) Takeshi Iwasa, Toshiya Matsuzaki, Naoko Tanaka, Masahiro Minakuchi, Fumi Shimizu, Rie Ogata, Machiko Kiyokawa, Masahiko Maegawa, Toshiyuki Yasui, Akira Kuwahara and Minoru Irahara :
Comparison and problems of measured values of LH, FSH, and PRL among measurement systems.,
Endocrine Journal, Vol.53, No.1, 101-109, 2006.

(要約): Several measurement systems are used for LH, FSH, and PRL in Japan, but the comparison of the measured values is difficult in some cases. In this study, we compared the measured values of 3 types of measurement systems widely used in Japan, SPAC-S, ARCHITECT, and Centaur, in females with a normal menstrual cycle and with various ovulatory disorders. Variant LH was discriminated by the criterion, SPAC-S LH/ARCHITECT LH ratio <0.5. Excluding the variant LH, the correlation of the measured LH values among the measurement systems was high, 0.94-0.99, and the value measured by ARCHITECT LH and Centaur LH was slightly higher than that measured by SPAC-S LH. As for FSH, the correlation was also high, 0.94-1.00, and the value measured by ARCHITECT FSH and Centaur FSH was slightly lower than that measured by SPAC-S FSH. The important judgment criterion of polycystic ovary syndrome (PCOS), a high level of LH relative to FSH, has been set to 1.0 or higher LH/FSH using SPAC-S. When the regression line of the LH/FSH ratio among the measurement systems was investigated, it can be said that 1.25 or higher by ARCHITECT and 1.40 or higher by Centaur are appropriate for the judgment criteria of a high level of LH relative to FSH. As for PRL, the correlation was high, 0.96-0.99, but the value showed large differences among the systems. For the diagnosis of hyperprolactinemia, the higher limit of the normal range may be 15 ng/mL for SPAC-S, 30 ng/mL for ARCHITECT, and 24 ng/mL for Centaur.
(キーワード): Adolescent / Adult / Aged / Chemiluminescent Measurements / Female / Follicle Stimulating Hormone / Humans / Hyperprolactinemia / Immunoradiometric Assay / Luteinizing Hormone / Middle Aged / Polycystic Ovary Syndrome / Pregnancy / Prolactin / Reference Standards / Regression Analysis
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.53.101
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16543679; ● Summary page in Scopus @ Elsevier: 2-s2.0-33644999382

(DOI: 10.1507/endocrj.53.101, PubMed: 16543679, Elsevier: Scopus) Hirokazu Uemura, Toshiyuki Yasui, Y Umino, M Yamada, Akira Kuwahara, Toshiya Matsuzaki, M Maegawa and Minoru Irahara :
Regulatory factors on parathyroid hormone-related peptide production by primary culture of lactating rat mammary gland.,
Hormone and Metabolic Research, Vol.37, No.8, 463-467, 2005.

(要約): Parathyroid hormone-related protein (PTHrP) is a major cause of humoral hypercalcemia of malignancy, but has also been widely found in fetal and adult non-neoplastic tissues. Lactating mammary gland has been shown to produce large amounts of PTHrP, and high levels of PTHrP have been measured in milk. We have examined the influences of several substances on the secretion of two different forms of PTHrP by primary cultures of mammary cells isolated from lactating rats to examine the regulatory mechanisms of PTHrP production by mammary cells. Primary cultures of mammary cells seeded at a density of 10(5) cells per 35 mm culture dish were grown on collagen gels. First, after cells were left 24 hours for attachment and incubated in 2 % FCS containing medium with for 12 hours, PTHrP (1 - 87) secretions were measured in conditioned medium with hormone supplementation for 1, 24 and 48 hours. Progesterone (10(-7) - 10(-5) mol/l) significantly suppressed PTHrP (1 - 87) secretion in a dose-dependent manner (p < 0.01), while 17beta-estradiol had no influence on PTHrP (1 - 87) secretion. Prolactin, a known stimulator of PTHrP expression in vivo, had no effect in this in vitro model. Second, PTHrP (1 - 34) secretion levels from confluent lactating mammary cells for 24 hours were evaluated. The same results were obtained in the case of PTHrP (1 - 87) secretion from non-confluent cells. Furthermore, dexamethasone (10(-6) mol/l) significantly suppressed PTHrP (1 - 34) secretion (p < 0.01). These results suggest that PTHrP production from the lactating mammary gland is suppressed by progesterone as well as dexamethasone. Progesterone dramatically falls after delivery, thus possibly accelerating PTHrP production by lactating mammary glands and resulting in considerable amounts of PTHrP secreted into the milk.
(キーワード): Animals / Dose-Response Relationship, Drug / Estradiol / Female / Fetal Diseases / Humans / Hypercalcemia / Lactation / Mammary Glands, Animal / Neoplasms / Parathyroid Hormone-Related Protein / Pregnancy / Progesterone / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-2005-870316
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16138257; ● Search Scopus @ Elsevier (PMID): 16138257; ● Search Scopus @ Elsevier (DOI): 10.1055/s-2005-870316

(DOI: 10.1055/s-2005-870316, PubMed: 16138257) 上村浩一, 安井敏之, 海野友香, 山田正代, 苛原稔 :
閉経後婦人の可溶型破骨細胞分化因子(Soluble RANKL)と破骨細胞分化抑制因子(Osteoprotegerin)の血中動態に関する検討,
Osteoporosis Japan, Vol.13, No.3, 609-612, 2005年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522543655765904512

(CiNii: 1522543655765904512) Hirokazu Uemura, M Kamada, M Maegawa, Y Ohmoto, K Murata, Akira Kuwahara, Toshiya Matsuzaki, Toshiyuki Yasui, T Takeji and Minoru Irahara :
Effect of hormone replacement therapy on the production of bone-resorbing cytokines by peripheral blood cells in postmenopausal women.,
Hormone and Metabolic Research, Vol.37, No.4, 226-230, 2005.

(要約): We studied the effects of hormone replacement therapy (HRT) with estrogen on postmenopausal changes in the production of bone-resorbing cytokines interleukin 1 beta (IL-1beta) and tumor necrosis factor alpha (TNFalpha). Both cytokines were measured in the supernatants of lipopolysaccharide (LPS)-stimulated whole-blood cells from 72 untreated and 44 HRT-treated women by ELISA. The levels of IL-1beta were significantly higher in women in their 40s and 50s and in postmenopausal women than in women in their teens, 20s and 30s, while the levels of TNFalpha did not show any changes related to age. Both levels in HRT-treated women were significantly lower than those in untreated women at almost every postmenopausal stage. In a prospective study, HRT induced significant declines in both levels. These results show that estrogen decreases the accelerated production of IL-1beta and reduces the production of TNFalpha in postmenopausal women at each postmenopausal stage, even in late-postmenopausal women.
(キーワード): Adult / Aging / Blood Cells / Bone Resorption / Cross-Sectional Studies / Cytokines / Estrogen Replacement Therapy / Female / Humans / Lipopolysaccharides / Middle Aged / Prospective Studies / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-2005-861382
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15952082; ● Search Scopus @ Elsevier (PMID): 15952082; ● Search Scopus @ Elsevier (DOI): 10.1055/s-2005-861382

(DOI: 10.1055/s-2005-861382, PubMed: 15952082) 有内和代, 楊河宏章, 宮本登志子, 井村光子, 西矢昌子, 中西りか, 苛原稔 :
治験の品質向上を目的としたミーティングチェックリストの作成,
JNI : The Journal of Nursing Investigation, Vol.3, No.2, 99-105, 2005年.

(要約): 治験は医薬品の開発において必須であり，その過程では逸脱を防ぎ，データの質を確保することが必要である．CRC(clinical research coordinator)の立場から，治験依頼者と医療機関の意思疎通のためのミーティングをより効率的に活用し，治験の品質の向上を行うためのチェックリストを作成した． T大学病院臨床試験管理センターのCRCがこれまでのミーティングにおいて経験した問題点を抽出し，それらを統合してミーティングチェックリストの原案を作成した．原案を平成16年5月∼6月に治験が開始された6件において主担当CRCが使用し，必要時には修正，補充を行い，それらを追記内容として直接チェックリストに記載した．治験開始後にこれらのチェックリストを回収し，追記内容等について検討した．詳細な追記は，除外基準や費用の負担(特定療養費の期間に関する規定，入院時の取り扱いなど)などの項目で見られた．画像の取り扱い，同種同効薬と併用禁止薬，条件付き可能薬の一般名表記等においても指摘があった．追加項目として受託事例数が，修正項目として表記方法やレイアウトについての意見があった．これらを総合的に評価し，またあまりに詳細なリストは実用的ではないという意見なども取り入れ，除外基準は疾患名を列記しチェックする形に，また検査，投薬に関しては詳しい内容とするなどの変更を加え，最終的なチェックリストとした．治験における逸脱の原因として，治験依頼者，治験責任医師の間での記載内容の解釈の微妙な不一致の関与の可能性が指摘されている．その克服にはCRCが中心となった品質保証のシステム化が必要であり，今回作成したチェックリストの活用などが有用と考えられる．今後は逸脱防止を目的とし，チェックリストの改良や活用法の検討を重ね対応するとともに，チェックリスト使用の有用性に関する評価を行う予定である．
(キーワード): 治験コーディネーター / チェックリスト / 臨床試験 / 逸脱
(徳島大学機関リポジトリ): ● Metadata: 113108
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050001338039304704

(徳島大学機関リポジトリ: 113108, CiNii: 1050001338039304704) Toshiyuki Yasui, Yuka Umino, Masaya Takikawa, Hirokazu Uemura, Akira Kuwahara, Toshiya Matsuzaki, Masahiko Maegawa, Hiroyuki Furumoto, Masakazu Miura and Minoru Irahara :
Effects of postmenopausal hormone therapy every day and every other day on lipid levels according to difference in body mass index,
Menopause, Vol.12, No.2, 223-231, 2005.

(要約): The objective of this study was to determine the effects of postmenopausal estrogen and progestogen therapy (EPT) every day and every other day on lipid levels, particularly triglyceride (TG) levels, according to difference in body mass index (BMI). Ninety-nine postmenopausal women (mean age, 53.9 +/- 5.6 years; mean BMI, 22.8 +/- 2.8 kg/m) were randomly treated with EPT every other day or every day for 1 year. Fifty women received oral administration of 0.625 mg of conjugated equine estrogen (CEE) and 2.5 mg of medroxyprogesterone acetate (MPA) every other day, and 49 women received oral administration of 0.625 mg of CEE and 2.5 mg of MPA every day. Blood samples were collected at baseline and after 1 year of therapy for measurement of fasting TG, total cholesterol, high-density lipoprotein-cholesterol (HDL-C), and apolipoproteins. Data from 88 of the 99 postmenopausal women were used for analysis. In women whose BMI was 25 kg/m or higher, TG levels during EPT every day increased by 26.8%, while TG levels during EPT every other day decreased by 12.3%. There was a significant (P < 0.05) difference between percentage changes in TG during EPT every day and every other day. In women whose BMI was less than 25 kg/m, TG levels during EPT every day increased by 21.7%, while during EPT every other day TG levels did not change. The mean levels of estradiol during EPT every day in women whose BMI was less than 25 kg/m and in women whose BMI was 25 kg/m or higher were 28.5 and 38.7 pg/mL, respectively, the difference between these levels was significant (P < 0.01). On the other hand, there was no significant difference between levels of estradiol during EPT every other day in these two BMI groups. Triglyceride levels during EPT every day with conventional doses of CEE and MPA increased more in overweight and obese postmenopausal women in association with increased estrogen levels.
(キーワード): Postmenopausal hormone therapy / Estrogen / Triglyceride / BMI
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15772571; ● Search Scopus @ Elsevier (PMID): 15772571

(PubMed: 15772571) Toshiyuki Yasui, Hirokazu Uemura, Yuka Umino, Masaya Takikawa, Seiichiro Saito, Akira Kuwahara, Toshiya Matsuzaki, Masahiko Maegawa, Hiroyuki Furumoto, Masakazu Miura and Minoru Irahara :
Serum estrogen level after hormone replacement therapy and body mass index in postmenopausal and bilaterally ovariectomized women,
Maturitas, Vol.50, No.1, 19-29, 2005.

(要約): The objective of this study was to determine the relationships of serum estrogen levels after hormone replacement therapy (HRT) every other day and every day with body mass index (BMI) in postmenopausal and bilaterally ovariectomized women. Eighty-six postmenopausal and 51 bilaterally ovariectomized women who had been suffering from vasomotor symptoms such as hot flush or atrophy of the vagina were randomly treated with HRT every other day or every day. Seventy-four patients received oral administration of 0.625 mg conjugated equine estrogen (CEE) and 2.5 mg medroxyprogesterone acetate (MPA) every other day, and 63 patients received oral administration of 0.625 mg CEE and 2.5 mg MPA every day as conventional HRT. Eighty-four postmenopausal and 50 bilaterally ovariectomized women completed this study. Serum estradiol levels after HRT every day in postmenopausal and bilaterally ovariectomized women were significantly (P <0.05 and <0.01, respectively) correlated with BMI, while those after HRT every other day were not correlated with BMI. The differences between estradiol levels after 12 months of treatment and initial estradiol levels were also significantly (P <0.01) correlated with BMI in both postmenopausal and bilaterally ovariectomized women who received HRT every day but not in women who received HRT every other day. Serum estrone level after HRT every day and the difference between estrone level after 12 months of treatment and initial estrone level were significantly (P <0.05 and <0.01, respectively) correlated with BMI only in bilaterally ovariectomized women. Serum estradiol levels after HRT every day increase more in overweight women than in non-overweight postmenopausal and bilaterally ovariectomized women. The results of the present study regarding the relationship between serum estradiol levels after HRT and BMI should be useful for selecting dosages of drugs to be used in HRT.
(キーワード): Serum estrogen level / Hormone replacement therapy / Ovariectomized women
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.maturitas.2004.03.013
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15590210; ● Search Scopus @ Elsevier (PMID): 15590210; ● Search Scopus @ Elsevier (DOI): 10.1016/j.maturitas.2004.03.013

(DOI: 10.1016/j.maturitas.2004.03.013, PubMed: 15590210) 上村浩一, 安井敏之, 海野友香, 滝川稚也, 苛原稔 :
低用量HRT施行中の腰椎骨密度の長期的推移, --- 結合型エストロゲンと酢酸メドロシキプロゲステロンの隔日投与の場合 ---,
産婦人科の実際, Vol.53, No.12, 1951-1955, 2004年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523669555995243904

(CiNii: 1523669555995243904) Koji Nakagawa, Shuji Yamano, Masaharu Kamada, Masahiko Maegawa, Akira Tokumura, Minoru Irahara and Hidekazu Saito :
Sperm-immobilizing antibodies suppress an increase in the plasma membrane fluidity of human spermatozoa,
Fertility and Sterility, Vol.82, No.Supplement 3, 1054-1058, 2004.

(要約): To clarify the mechanism by which capacitation is blocked by sperm-immobilizing antibodies, changes in the plasma membrane fluidity of human spermatozoa exposed to sperm-immobilizing antibodies were evaluated. In vitro cell culture study using human spermatozoa. Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima. Semen samples were obtained from four healthy, fertile volunteers. The internalization of [3H]lyso-platelet activating factor (lyso-PAF) across the plasma membranes of human spermatozoa, which were exposed to sperm-immobilizing antibodies (antisperm group) or not exposed (control group), was measured at 20 and 60 minutes after the addition of a phospholipid probe using the modified albumin-back extraction method. The percentage of internalization of [3H]lyso-PAF across the plasma membrane of human spermatozoa. Although the percentages of internalization of [3H]lyso-PAF (mean +/- SE) in the antisperm and control groups 20 minutes after addition of [3H]lyso-PAF were not significantly different (6.6% +/- 1.5% and 9.2% +/- 2.1%, respectively), at 60 minutes after the addition, the percentage in the antisperm group (9.0% +/- 1.3%) was significantly lower than that in the control group (13.4% +/- 1.3%). This inhibitory effect was diminished when spermatozoa exposed to sperm-immobilizing antibodies were incubated in an antibody-free medium. Sperm-immobilizing antibodies suppress the increase in internalization of an alkyl ester lysophospholipid probe in plasma membranes of human spermatozoa, and this inhibitory effect is reversible. Therefore, sperm-immobilizing antibodies suppress the fluidity of the plasma membranes of human spermatozoa, thus blocking capacitation.
(キーワード): Capacitation / internalization / flip-flop movement / membrane fluidity / sperm-immobilizing antibody
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.fertnstert.2004.03.034
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15474073; ● Search Scopus @ Elsevier (PMID): 15474073; ● Search Scopus @ Elsevier (DOI): 10.1016/j.fertnstert.2004.03.034

(DOI: 10.1016/j.fertnstert.2004.03.034, PubMed: 15474073) 安井敏之, 海野友香, 滝川稚也, 上村浩一, 苛原稔 :
隔日投与によるホルモン補充療法の再考,
Osteoporosis Japan, Vol.12, No.3, 95-98, 2004年. 前川正彦, 苛原稔 :
EBMからみた不妊治療の実際, --- 高齢不妊患者の治療方針 ---,
産婦人科治療, Vol.88, No.5, 1141-1147, 2004年.

(キーワード): 高齢女性 / 卵巣予備能 / low responder / 加齢
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523106605692854656

(CiNii: 1523106605692854656) 井村光子, 楊河宏章, 宮本登志子, 乾加代子, 中西りか, 西矢昌子, 苛原稔 :
治験コーディネーター業務における看護師の専門性の意義に関する検討,
JNI : The Journal of Nursing Investigation, Vol.2, No.1, 21-26, 2004年.

(要約): 治験の質の向上に治験コーディネーター(CRC)業務の充実は不可欠である．今回，CRC業務における看護師としての専門性を解析し，さらに専門性をどのようにCRC 業務の質向上に結びつけるかに関して検討した．進行期肺癌に対する抗癌剤の治験(2002．2∼2003．5)を対象に，実際にCRCが行った支援業務の中で，看護師としての専門性が有意義であった項目を解析した． CRC業務を被支援者別に分類すると，被験者20，医師22，他部署19項目となった．うち5項目(インフォムドコンセント同席/説明補助，診察時同席/介助，観察/計測，情報提供，スケジュール管理)が2被支援者にまたがる支援内容であり，いずれも被験者に接する場での業務であった．個々の項目についてCRCの意義を検討すると，この治験は末期癌の患者を対象としていたため，疾患が憎悪するのではないかという不安の中で，同意取得時と4週毎の効果判定時の心理的サポートが，治験の遂行におけるCRCの大きな役割であった．このような治験対象患者への心理的サポートは看護師だからこそできる業務であり，そこにCRC活動における看護師の専門性の意義が認められた．以上より，被験者に接する場での治験支援活動は，特に看護師の専門性を活かし，治験の質を向上させうるCRC業務であると考えられた．
(キーワード): CRC / 看護師の専門性 / 臨床試験 / 治験
(徳島大学機関リポジトリ): ● Metadata: 113088
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050001338017587200

(徳島大学機関リポジトリ: 113088, CiNii: 1050001338017587200) Shuji Yamano, Jun Yamazaki, Minoru Irahara, Akira Tokumura, Koji Nakagawa and Hidekazu Saito :
Human spermatozoa capacitated with progesterone or a long incubation show accelerated internalization by an alkyl ether lysophospholipid,
Fertility and Sterility, Vol.81, No.3, 605-610, 2004.

(要約): To evaluate changes that occur in sperm plasma membranes during capacitation, the internalization of [(3)H]lyso-platelet activating factor ([(3)H]lyso-PAF) across the plasma membrane of human spermatozoa was measured as a function of incubation time or exposure to progesterone (P). In vitro cell culture study using human spermatozoa. Department of Obstetrics and Gynecology, School of Medicine, the University of Tokushima, Japan. Semen were obtained from three fertile healthy volunteers. The internalization of [(3)H]lyso-PAF across the plasma membranes of human spermatozoa that were incubated for an extended period or exposed to P was measured at 5, 20, 60, and 120 minutes after the addition of the phospholipid probe using the modified albumin back-exchange method. The percentage of capacitated and acrosome-reacted sperm and the proportion of internalization of lyso-PAF across the plasma membrane. A 6-hour incubation period significantly increased the percentage of capacitated spermatozoa and the proportion of internalization of [(3)H]lyso-PAF across the plasma membrane of human spermatozoa compared with controls (capacitated spermatozoa, 20.3 +/- 10.6% vs. 8.5 +/- 1.8%; internalization 120 minutes after the addition of the phospholipid probe, 25.6 +/- 2.5% vs. 11.6 +/- 3.0%) (mean +/- SEM). Exposure to P significantly increased the percentage of capacitated spermatozoa compared with controls (19.6 +/- 6.8% vs. 11.0 +/- 2.4%) and also significantly accelerated the internalization of [(3)H]lyso-PAF compared with controls (internalization 120 minutes after the addition of the phospholipid probe, 26.2 +/- 1.8% vs. 21.4 +/- 1.1%). The administration of P or a long incubation increased the proportion of internalization and consequently induced capacitation in human spermatozoa.
(キーワード): Cell Membrane / Cells, Cultured / Humans / Male / Platelet Activating Factor / Progesterone / Sperm Capacitation / Spermatozoa / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.fertnstert.2003.07.036
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15037409; ● Search Scopus @ Elsevier (PMID): 15037409; ● Search Scopus @ Elsevier (DOI): 10.1016/j.fertnstert.2003.07.036

(DOI: 10.1016/j.fertnstert.2003.07.036, PubMed: 15037409) 前川正彦, 山本哲史, 苛原稔 :
精子ー透明帯相互作用について, --- ビアコアシステムを用いたアプローチ ---,
産婦人科の実際, Vol.53, No.1, 105-110, 2004年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521417756180557568

(CiNii: 1521417756180557568) Toshiyuki Yasui, Hirokazu Uemura, Yuka Umino, Masaya Takikawa, Akira Kuwahara, Seiichiro Saito, Toshiya Matsuzaki, Masahiko Maegawa, Hiroyuki Furumoto, Masakazu Miura and Minoru Irahara :
Serum estradiol concentration as measured by HPLC-RIA and bone mineral density in postmenopausal women during hormone replacement therapy,
Hormone Research, Vol.61, No.3, 117-125, 2004.

(要約): To determine the relationship between the serum estradiol concentration and bone mineral density (BMD) of the lumbar spine in postmenopausal women treated with conjugated equine estrogen (CEE) and medroxyprogesterone acetate (MPA) every other day and every day. Eighty-four postmenopausal women were randomly treated with hormone replacement therapy (HRT) every other day and every day. Forty-seven women received oral administration of 0.625 mg CEE and 2.5 mg MPA every other day, and 37 women received oral administration of 0.625 mg CEE and 2.5 mg MPA every day. BMD of the lumbar spine at 12 months and serum concentrations of estradiol and estrone at 6 and 12 months after treatment were examined. The estradiol concentration in subjects treated every other day showed a significant (p < 0.001) positive correlation with the percentage change in lumbar BMD, while that in subjects treated every day was not correlated with the percentage change in BMD. The differences between serum estradiol concentrations after 12 months of treatment and initial serum estradiol values in women treated every other day and every day also showed a significant (p < 0.01 and 0.05, respectively) positive correlation with percentage changes in BMD. In women treated every other day, body mass index (BMI) in the subjects in whom BMD did not increase was significantly (p < 0.01) lower than that in the subjects in whom BMD did increase. The serum estradiol concentration in women treated every other day has a strong positive correlation with the percentage change in lumbar BMD, but a higher estradiol concentration may be needed for women in whom BMD did not increase with HRT every other day after due consideration of individual characteristics such as BMI.
(キーワード): Hormone replacement therapy / Estradiol / Bone
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000075523
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14676459; ● Summary page in Scopus @ Elsevier: 2-s2.0-12144289071

(DOI: 10.1159/000075523, PubMed: 14676459, Elsevier: Scopus) 前川正彦, 苛原稔 :
EBMからみた不妊治療の実際, --- 不育症とその治療方針 ---,
産婦人科治療, Vol.87, No.5, 602-607, 2003年.

(キーワード): 不育症 / 習慣流産 / 抗リン脂質抗体症候群 / 免疫療法

Hirokazu Uemura, Toshiyuki Yasui, Yuka Umino, Hirobumi Niki, Masaya Takikawa, Seiichiro Saito, Hiroyuki Furumoto and Minoru Irahara :
Circulating osteoprotegerin in women during GnRH-agonist treatment and their relationships with mineral components and biomarkers of bone turnover,
Bone, Vol.33, No.5, 860-866, 2003.

(要約): A novel cytokine termed osteoprotegerin (OPG) that is also called osteoclastogenesis-inhibitory factor, which inhibits osteoclast maturation and activity, was recently isolated. In order to determine the influence of estrogen deficiency on the levels of circulating OPG in women, we studied the changes in the levels of circulating OPG in 10 Japanese women ages 25-49 (mean +/- SD, 34.0 +/- 6.9) years with endometriosis receiving gonadotropin-releasing hormone agonists (GnRH-a) therapy. We further analyzed whether the levels of circulating OPG have relations with the levels of the biomarkers of bone turnover or those of circulating mineral components in these patients during GnRH-a treatment. The patients were treated with a monthly injection of 3.75 mg leuprolide acetate depot for 6 months. In all patients, the concentrations of serum estradiol decreased after 6 months of GnRH-a treatment. The bone mineral density of the lumber spines in these patients significantly (P < 0.01) decreased (percentage change: mean +/- SD, -5.4 +/- 2.1%), while circulating OPG levels significantly (P < 0.01) increased after 6 months of treatment. The values of circulating OPG had significant correlations with those of urinary pyridinoline (r = 0.59, P < 0.01), urinary deoxypylridinoline (Dpd) (r = 0.46, P < 0.05), and serum alkaline phosphatase (r = 0.66, P < 0.01) but not with those of serum carboxy-terminal propeptide of type I procollagen during GnRH-a treatment. The values of circulating OPG also correlated significantly with those of serum calcium (Ca) and phosphorus (P) (r = 0.65 and 0.72, P < 0.01). Further analyses revealed that the percentage change in the value of circulating OPG had a significant correlation with that of urinary Dpd (r = 0.84, P < 0.01). These results suggest that circulating OPG levels rise against the increase in osteoblastic bone resorption and circulating Ca levels in the case of estrogen deficiency, possibly as a compensatory mechanism serving to limit circulating Ca levels and bone density.
(キーワード): Adult / Bone Density / Bone Remodeling / Endometriosis / Female / Glycoproteins / Gonadotropin-Releasing Hormone / Humans / Leuprolide / Middle Aged / Osteoprotegerin / Receptors, Cytoplasmic and Nuclear / Receptors, Tumor Necrosis Factor / Statistics, Nonparametric
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bone.2003.08.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14623063; ● Search Scopus @ Elsevier (PMID): 14623063; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bone.2003.08.001

(DOI: 10.1016/j.bone.2003.08.001, PubMed: 14623063) Toshiyuki Yasui, Hirokazu Uemura, Masaya Takikawa and Minoru Irahara :
Hormone replacement therapy in postmenopausal women.,
The Journal of Medical Investigation : JMI, Vol.50, No.3-4, 136-145, 2003.

(要約): Hormone replacement therapy (HRT) is effective for relieving vasomotor symptoms such as hot flash and vaginal atrophy and for preventing bone loss in postmenopausal and bilaterally ovariectomized women. However, compliance with HRT was reported to be low despite the benefits of HRT. In addition, results of several recent large-scale randomized clinical trials have demonstrated that protection from cardiovascular disease is not an indication for treatment with estrogen and progestin in postmenopausal women. Recent studies have demonstrated that low-dose HRT is safe and effective for prevention of postmenopausal bone loss. Low-dose HRT has also been shown to be effective for reducing the number and severity of hot flashes, improving vaginal atrophy, and inducing favorable changes in lipids, lipoproteins and hemostatic factors. Moreover, low-dose regimens of CEE (conjugated equine estrogen) and MPA (medroxyprogesterone acetate) result in higher rates of amenorrhea and endometrial protection compared with the conventional dose of HRT. Low-dose HRT may improve the compliance rate and may be more effective than conventional-dose HRT for reducing the risk of breast cancer. On the other hand, it has been shown that transdermal estrogen treatment reduces the incidence and severity of hot flashes and that long-term treatment with transdermally administered estrogen is effective for protection against osteoporosis. Transdermal administration of estrogen is recommended in postmenopausal women with hypertriglycemia because this treatment has little effect on lipid metabolism. The serum estradiol level was reported to be closely related to estrogenic effects on various tissues. An HRT regimen should be based on the needs of each patient. Serum estradiol levels in women should be maintained at appropriate levels for benefits and not be excessively high in order to prevent side effects. Selection of the most appropriate regimen of HRT (dose, route of administration and schedule) for the needs of the individual are important factors to increase the rate of continuation with HRT.
(キーワード): Administration, Cutaneous / Atrophy / Breast Neoplasms / Estrogen Replacement Therapy / Estrogens, Conjugated (USP) / Female / Hot Flashes / Humans / Medroxyprogesterone Acetate / Osteoporosis, Postmenopausal / Postmenopause / Randomized Controlled Trials as Topic / Risk / Vagina
(徳島大学機関リポジトリ): ● Metadata: 110688
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 13678382; ● Search Scopus @ Elsevier (PMID): 13678382

(徳島大学機関リポジトリ: 110688, PubMed: 13678382) 前川正彦, 山本哲史, 苛原稔 :
妊娠による母体免疫系の変化,
臨床婦人科産科, Vol.57, No.8, 1012-1016, 2003年. 安井敏之, 海野友香, 尾形理江, 清川麻知子, 上村浩一, 滝川稚也, 桑原章, 松崎利也, 前川正彦, 苛原稔, 牛越賢治郎 :
ラット卵巣細胞培養系におけるCINC産生に及ぼす温経湯の作用機序に関する検討,
産婦人科漢方研究のあゆみ, Vol.20, 108-112, 2003年. 安井敏之, 海野友香, 滝川稚也, 上村浩一, 苛原稔, 三浦雅一 :
自然閉経後ならびに両側卵巣摘出後婦人における内因性エストロゲン濃度と腰椎骨密度の比較検討,
Osteoporosis Japan, Vol.11, No.3, 107-110, 2003年. 滝川稚也, 安井敏之, 海野友香, 上村浩一, 苛原稔 :
隔日投与によるホルモン補充療法施行中の腰椎骨密度の長期的変化,
Osteoporosis Japan, Vol.11, No.3, 131-133, 2003年. 上村浩一, 安井敏之, 滝川稚也, 苛原稔 :
血中エストロゲン濃度の変動による血中osteoprotegerin(OPG)濃度の推移の検討,
Osteoporosis Japan, Vol.11, No.3, 95-98, 2003年. Toshiyuki Yasui, Toshiya Matsuzaki, Kenjiro Ushigoe, Akira Kuwahara, Masahiko Maegawa, Hiroyuki Furumoto, Toshihiro Aono and Minoru Irahara :
Stimulatory Effect of the Herbal Medicine Keishi-bukuryo-gan on a Cytokine-induced Neutrophil Chemoattractant,in Rat Ovarian Cell Culture,
American Journal of Reproductive Immunology, Vol.50, No.1, 90-97, 2003.

(要約): We investigated the effects of Keishi-bukuryo-gan, a Japanese herbal medicine, and its crude ingredients in relation to the production of cytokine-induced neutrophil chemoattractant (CINC/gro), interleukin 1beta (IL-1beta) and tumor necrosis factor alpha (TNFalpha), which are known to stimulate the secretion of CINC/gro in the ovulatory process, and the effects of Keishi-bukuryo-gan with those of Toki-shakuyaku-san, which has been shown to have an effect on the ovary. We cultured whole ovarian dispersates from immature (3-week-old) female rats with Keishi-bukuryo-gan, Toki-shakuyaku-san and crude ingredients of Keishi-bukuryo-gan. The contents of CINC/gro, IL-1beta and TNFalpha in the cultured media were determined by enzyme-linked immunosorbent assay (ELISA). Keishi-bukuryo-gan stimulated the secretion of CINC/gro in a dose-dependent manner, and the secretion of CINC/gro into the culture medium increased significantly at concentrations of Keishi-bukuryo-gan of 10 and 100 microg/mL (P < 0.001). The stimulatory effect of Keishi-bukuryo-gan on the production of CINC/gro is significantly (P < 0.001) stronger than that of Toki-shakuyaku-san at the same concentrations of 100 microg/mL. In addition, Keishi-bukuryo-gan stimulated the secretion of IL-1beta in a dose-dependent manner, while it did not stimulate the secretion of TNFalpha even at a concentration of 100 microg/mL. Moutan Cortex, Paeoniae Radix and Persicae Semen, which are crude ingredients of Keishi-bukuryo-gan, enhanced the secretion of CINC/gro significantly (P < 0.01) in cultured whole ovarian dispersates. These results show that Keishi-bukuryo-gan can stimulate the secretion of CINC/gro as well as the production of IL-1beta and that this stimulatory effect of Keishi-bukuryo-gan was significantly stronger than that of Toki-shakuyaku-san in immature rat ovarian cell culture.
(キーワード): Animals / Cells, Cultured / Chemokine CXCL1 / Chemokines, CXC / Cinnamomum / Dose-Response Relationship, Drug / Drugs, Chinese Herbal / Female / Intercellular Signaling Peptides and Proteins / Interleukin-1 / Ovary / Paeonia / Plant Extracts / Plants, Medicinal / Rats / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1034/j.1600-0897.2003.00055.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14506933; ● Search Scopus @ Elsevier (PMID): 14506933; ● Search Scopus @ Elsevier (DOI): 10.1034/j.1600-0897.2003.00055.x

(DOI: 10.1034/j.1600-0897.2003.00055.x, PubMed: 14506933) 安井敏之, 田中優, 海野友香, 滝川稚也, 上村浩一, 苛原稔, 三浦雅一 :
ホルモン補充療法中の骨密度の増加に必要な血中エストラジオール濃度の検討,
産婦人科の実際, Vol.52, No.5, 631-636, 2003年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520854806226352512

(CiNii: 1520854806226352512) Toshiyuki Yasui, Toshiya Matsuzaki, Rie Ogata, Machiko Kiyokawa, Kenjiro Ushigoe, Hirokazu Uemura, Akira Kuwahara, Hiroshi Ikawa, Masahiko Maegawa, Hiroyuki Furumoto, Toshihiro Aono and Minoru Irahara :
The herbal medicine Unkei-to stimulates the secretion of a cytokine-induced neutrophil chemoattractant, CINC/gro, in the rat ovarian cell culture,
American Journal of Reproductive Immunology, Vol.49, No.1, 14-20, 2003.

(要約): We investigated the ovulation-inducing effects of Unkei-to, a Japanese herbal medicine, in relation to the production of sex steroid hormones (17beta-estradiol and progesterone), cytokine-induced neutrophil chemoattractant (CINC/gro), interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) in the rat ovarian cell culture. Unkei-to at a concentration of 100 microg/mL significantly stimulated the secretions of 17beta-estradiol and progesterone (P < 0.01) in cultured whole ovarian dispersates. Unkei-to also enhanced the secretion of CINC/gro in a dose-dependent manner, and the secretions of CINC/gro increased significantly at concentrations of 10 and 100 microg/mL (P < 0.01). These stimulatory effects of Unkei-to on steroidgenesis and CINC/gro production are very similar to those of another Japanese herbal medicine, Toki-Shakuyaku-san. In addition, Unkei-to significantly (P < 0.01) enhanced the secretions of both IL-1beta and TNF-alpha, which are known to stimulate the secretion of CINC/gro in the ovulatory process, at concentrations of 10 and 100 microg/mL. The stimulatory effect of Unkei-to at a concentration of 100 microg/mL on IL-1beta/was significantly (P < 0.01) lower than that of Toki-Shakuyaku-san, while the stimulatory effects of these two herbal medicines at a concentration of 100 microg/mL on TNF-alpha were similar. These results show that Unkei-to can stimulate ovarian steroidgenesis and the ovulatory process by inducing the secretion of CINC/gro with IL-1beta and TNF-alpha in vitro. Unkei-to has stimulatory effects on both steroidgenesis and the ovulatory process in the ovary as well as a stimulatory effect on the hypothalamus-pituitary axis, and it may be useful for treating patients with ovulatory disorders.
(キーワード): Cytokine-induced neutrophil chemo-attractant / interleukin-1β / ovulation / tumor necrosis factor-α / Unkei-to
(出版サイトへのリンク): ● Publication site (DOI): 10.1034/j.1600-0897.2003.01145.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12733590; ● Search Scopus @ Elsevier (PMID): 12733590; ● Search Scopus @ Elsevier (DOI): 10.1034/j.1600-0897.2003.01145.x

(DOI: 10.1034/j.1600-0897.2003.01145.x, PubMed: 12733590) Masayo Yamada, Toshiya Matsuzaki, Takeshi Iwasa, Fumi Shimizu, Naoko Tanaka, Rie Ogawa, Machiko Kiyokawa, Toshiyuki Yasui, Minoru Irahara and Toshihiro Aono :
Serum Leptin Levels in Women throughout Life;Relationship to Body Mass Index and Serum Estradiol Levels,
Japanese Journal of Reproductive Endocrinology, Vol.8, 55-60, 2003.

(キーワード): Leptin / Estradiol / Body mass index

Koji Nakagawa, Shuji Yamano, Yukari Tsutsumi, M Matsumoto, Kenji Hinokio, Minoru Irahara and Toshihiro Aono :
The scheduled ovarian hyperstimulation method makes it easy to perform ICSI with fresh testicular sperm (ICSI/TESE),
Archives of Andrology, Vol.48, No.5, 329-336, 2002.

(要約): The authors evaluated whether scheduled ovarian stimulation makes it easy to perform ICSI with fresh testicular sperm. Scheduled ovarian hyperstimulation was applied for testicular sperm extraction and ICSI with fresh testicular spermatozoa. Fifteen cycles in 10 couples were included in the present study; all couples were azoospermic, 5 were obstructive, and the remaining 5 were nonobstructive. No cycles were canceled, and all oocyte retrievals were performed on the scheduled day. Testicular sperm were obtained in 14 treatment cycles (93%). The mean numbers of retrieved and injected oocytes were 9.4 and 6.4, respectively. The fertilization and cleavage rates were 47 and 91%, respectively. Embryo transfers were performed in 12 cycles except 2 cycles that had no embryos. The number of transferred embryos was 2.3. Two clinical pregnancies were obtained. This scheduled ovarian hyperstimulation was applicable for ICSI with fresh testicular sperm.
(キーワード): Azoospermia / Icsi / Scheduled Ovarian Hyperstimulation / Sperm / Tese
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/01485010290099165
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12230818; ● Search Scopus @ Elsevier (PMID): 12230818; ● Search Scopus @ Elsevier (DOI): 10.1080/01485010290099165

(DOI: 10.1080/01485010290099165, PubMed: 12230818) Hirokazu Uemura, Toshiyuki Yasui, Machiko Kiyokawa, Akira Kuwahara, Hiroshi Ikawa, Toshiya Matsuzaki, Masahiko Maegawa, Hiroyuki Furumoto and Minoru Irahara :
Serum osteoprotegerin/osteoclastogenesis-Inhibitory factor during pregnancy and lactation and their relationships with calcium regulating hormones and bone turnover markers,
The Journal of Endocrinology, Vol.174, No.2, 353-359, 2002.

(要約): Pregnancy and lactation induce dynamic changes in maternal bone and calcium metabolism. A novel cytokine termed osteoprotegerin (OPG)/osteoclastogenesis-inhibitory factor (OCIF) was recently isolated; this cytokine inhibits osteoclast maturation. To define the effects of pregnancy and lactation on circulating OPG/OCIF in mothers, we studied the changes in the levels of OPG/ OCIF as well as those of calcium-regulating hormones and biochemical markers of bone turnover in the maternal circulation during pregnancy (at 8-11 weeks, at 22-30 weeks, at 35-36 weeks and immediately before delivery) and lactation (at 4 days and at 1 month postpartum). Serum intact parathyroid hormone levels did not change and were almost within the normal range in this period. In contrast, serum 1,25-dihydroxyvitamin D levels increased with gestational age and were above the normal range during pregnancy. After delivery, they fell rapidly and significantly (P<0.01) to the normal range. The levels of serum bone-specific alkaline phosphatase, one of the markers of bone formation, increased with gestational age. After delivery, these levels were further increased at 1 month postpartum. The levels at 1 month postpartum were significantly higher than those at 8-11 and 22-30 weeks of pregnancy (P<0.01 and P<0.05 respectively). The levels of serum C-terminal telopeptides of type I collagen, one of the markers of bone resorption, did not change during pregnancy. After delivery, they rapidly and significantly (P<0.01) rose at 4 days postpartum, and had then fallen by 1 month postpartum. Circulating OPG/OCIF levels gradually increased with gestational age and significantly (P<0.01) increased immediately before delivery to 1.40+/-0.53 ng/ml (means+/-S.D.) compared with those in the non-pregnant, non-lactating controls (0.58+/-0.11 ng/ml). After delivery, they fell rapidly to 0.87+/-0.27 ng/ml at 4 days postpartum and had fallen further by 1 month postpartum. These results suggest that the fall in OPG/OCIF levels may be partially connected with the marked acceleration of bone resorption after delivery.
(キーワード): Adult / Alkaline Phosphatase / Biological Markers / Bone Resorption / Calcium / Case-Control Studies / Collagen / Collagen Type I / Enzyme-Linked Immunosorbent Assay / Female / Glycoproteins / Humans / Lactation / Osteoprotegerin / Parathyroid Hormone / Peptides / Phosphorus / Pregnancy / Pregnancy Trimesters / Receptors, Cytoplasmic and Nuclear / Receptors, Tumor Necrosis Factor / Regression Analysis / Serum Albumin / Statistics, Nonparametric / Vitamin D
(出版サイトへのリンク): ● Publication site (DOI): 10.1677/joe.0.1740353
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12176675; ● Search Scopus @ Elsevier (PMID): 12176675; ● Search Scopus @ Elsevier (DOI): 10.1677/joe.0.1740353

(DOI: 10.1677/joe.0.1740353, PubMed: 12176675) 安井敏之, 手束典子, 山田正代, 上村浩一, 苛原稔, 青野敏博, 三浦雅一 :
閉経後婦人における内因性エストロゲン濃度と腰椎骨密度の検討,
Osteoporosis Japan, Vol.10, No.3, 76(444)-78(446), 2002年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520010380150481280

(CiNii: 1520010380150481280) 上村浩一, 清川麻知子, 安井敏之, 苛原稔 :
血中エストロゲン濃度の変動による血中osteoprotegerin(OPG)濃度の推移および骨代謝との関係の検討,
Osteoporosis Japan, Vol.10, No.3, 70-72, 2002年. 鎌田正晴, 前川正彦, 苛原稔, 山野修司 :
抗精子抗体と不妊,
産婦人科治療, Vol.84, No.6, 1082-1087, 2002年.

(キーワード): 抗精子抗体 / 免疫性不妊症 / 先体反応 / 受精

尾形理江, 安井敏之, 手束典子, 清川麻知子, 山田正代, 上村浩一, 桑原章, 井川洋, 松崎利也, 青野敏博, 苛原稔, 牛越賢治郎 :
ラット卵巣細胞培養系におけるケモカイン産生に及ぼす温経湯の効果に関する検討,
産婦人科漢方研究のあゆみ, Vol.19, 128-131, 2002年. Masahiko Maegawa, Masaharu Kamada, Minoru Irahara, Satoshi Yamamoto, Shuji Yoshikawa, Yuka Kasai, Yasukazu Ohmoto, Hironori Gima, Christian J Thaler and Toshihiro Aono :
A repertoire of cytokines in human seminal plasma,
Journal of Reproductive Immunology, Vol.54, No.1-2, 33-42, 2002.

(要約): The pathophysiological significance of seminal cytokines in sperm function is still controversial. We determined the repertoire of cytokines in seminal plasma obtained from men with or without abnormalities in semen and assessed the pathophysiological significance of seminal cytokines. After conventional analysis of semen samples obtained from 86 men, levels of seminal cytokines (interleukin [IL]-1alpha, IL-2, IL-4, IL-6, IL-8, tumor necrosis factor-alpha [TNF-alpha], interferon-gamma, granulocyte colony-stimulating factor [G-CSF], macrophage CFS [M-CSF]) and granulocyte elastase were measured by an enzyme-linked immunosorbent assay. Leukocytospermia was defined as seminal plasma, which has > or =1000 ng/ml granulocyte elastase. Leukocytospermia was found in nine of 62 of the subjects in the normozoospermic group but in none of the 24 subjects showing abnormal sperm parameters (azoospermia, n=5; oligozoospermia, n=4; asthenozoospermia, n=15). The IL-8 level in the leukocytospermic group was significantly higher than those in the normal and oligozoospermic groups. IL-1alpha and TNF-alpha levels in the leukocytospermic group were significantly higher than those in the normal and asthenozoospermic groups. Although the G-CSF level in the leukocytospermic group was significantly higher than that in the normal group, high levels of M-CSF were detected in all groups. The IL-8 level was strongly correlated with IL-1alpha (r=0.935, P<0.0001) and G-CSF (r=0.916, P<0.0001) levels. Cytokines detected in seminal plasma are associated with the pathogenesis of leukocytospermia but not with the pathogenesis of asthenozoospermia and oligozoospermia.
(キーワード): Granulocyte elastase / Leukocytospermia / Seminal plasma
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0165-0378(01)00063-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11839394; ● Search Scopus @ Elsevier (PMID): 11839394; ● Search Scopus @ Elsevier (DOI): 10.1016/S0165-0378(01)00063-8

(DOI: 10.1016/S0165-0378(01)00063-8, PubMed: 11839394) Mitsuhiro Kimura, Minoru Irahara, Toshiyuki Yasui, Seiichiro Saito, Michiko Tezuka, Shuji Yamano, Masaharu Kamada and Toshihiro Aono :
The Obesity in Bilateral Ovariectomized Rats is Related to a Decrease in the Expression of Leptin Receptors in the Brain,
Biochemical and Biophysical Research Communications, Vol.290, No.4, 1349-1353, 2002.

(要約): We investigated the expression levels of leptin receptors in the brain of ovariectomized (OVX) rats. The mean expression level of ob mRNA in adipose tissues of OVX rats was significantly (P < 0.01) lower than that in the SHAM operation group rats, and the mean body weight of OVX rats was significantly (P < 0.01) greater than that in the SHAM group rats. However, there were no differences between serum leptin concentrations in these two groups. The mean level of leptin receptor (OB-R) mRNA expression in the brain tissue and the mean level of long form type OB-R (OB-RL) mRNA expression in the hypothalamus of the OVX rats were significantly (P < 0.05) lower than those in the SHAM group rats. These changes were cancelled by supplementation with 17 beta-estradiol in OVX rats. These results suggested that not only changes in the expression level of ob mRNA in adipose tissue and the serum leptin concentration but also changes in the OB-R mRNA in the brain are involved in the body weight increase in OVX rats and that a decrease in OB-R makes transmission of signals to suppress the amount of food intake difficult, thus leading to an increase in body weight.
(キーワード): leptin receptor / long form / short form / estrogen
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.2002.6355
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11812012; ● Search Scopus @ Elsevier (PMID): 11812012; ● Search Scopus @ Elsevier (DOI): 10.1006/bbrc.2002.6355

(DOI: 10.1006/bbrc.2002.6355, PubMed: 11812012) Michiko Tezuka, Minoru Irahara, Koji Ogura, Machiko Kiyokawa, Takao Tamura, Toshiya Matsuzaki, Toshiyuki Yasui and Toshihiro Aono :
Effects of leptin on gonadotropin secretion in juvenile female rat pituitary cells,
European Journal of Endocrinology, Vol.146, No.2, 261-266, 2002.

(キーワード): BODY-MASS INDEX / NEUROPEPTIDE-Y / PUBERTAL STAGE / PLASMA LEPTIN / OBESE GENE / RECEPTOR / MICE / HYPOTHALAMUS / EXPRESSION / GROWTH
(出版サイトへのリンク): ● Publication site (DOI): 10.1530/eje.0.1460261
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11834438; ● Search Scopus @ Elsevier (PMID): 11834438; ● Search Scopus @ Elsevier (DOI): 10.1530/eje.0.1460261

(DOI: 10.1530/eje.0.1460261, PubMed: 11834438) Kenji Hinokio, Shuji Yamano, Koji Nakagawa, Minoru Irahara, Masaharu Kamada, Akira Tokumura and Toshihiro Aono :
Lysophosphatidic acid stimulates nuclear and cytoplasmic maturation of golden hamster immature oocytes in vitro via cumulus cells,
Life Sciences, Vol.70, No.7, 759-767, 2002.

(要約): Lysophosphatidic acid (LPA), a member of the phospholipid autacoid family, is induced in incubated human follicular fluid by lysophospholipase D. It is well known that LPA functions as a growth factor and the hypothesis that LPA in human follicular fluid takes a part in meiosis of oocytes is quite plausible. We studied the effects of LPA on the maturation of golden hamster immature oocytes in vitro. Hamster oocytes with a germinal vesicle were cultured in Tyrode's albumin lactate pyruvate (TALP) medium with 10(-5) M LPA, 10 ng/ml epidermal growth factor (EGF), 30 ng/ml insulin-like growth factor-1, 1 ng/ml tumor growth factor-alpha or 1 ng/ml basic fibroblast growth factor. The nuclear maturation rates in the LPA and EGF groups were significantly higher than in the control group and the other growth factors did not show any stimulatory effect (LPA group; 74.3% [75/101], EGF group; 82.4% [89/108] vs. control group; 60.2% [59/98], p < 0.05, p < 0.01, respectively). When the cells of cumulus were removed, EGF and LPA did not increase the nuclear maturation rates. Cotreatment EGF and LPA did not significantly enhance the stimulatory effect observed with LPA alone on maturation in vitro. The penetration rate determined by the zona-free hamster oocyte test was significantly higher in the LPA group than in the control group (26.7% vs. 13.2%, p < 0.05) and was comparable with that of oocytes matured in vivo. In conclusion, LPA stimulates the nuclear and cytoplasmic maturation of hamster immature oocytes via cumulus cells.
(キーワード): Animals / Cell Nucleus / Cricetinae / Cytoplasm / Drug Interactions / Epidermal Growth Factor / Female / Fertilization in Vitro / Lysophospholipids / Mesocricetus / Oocytes / Oogenesis / Tyrphostins / Virulence Factors, Bordetella
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11833739; ● Search Scopus @ Elsevier (PMID): 11833739

(PubMed: 11833739) Masahiko Maegawa, Masaharu Kamada, Satoshi Yamamoto, Shuji Yamano, Minoru Irahara, Hiroshi Kido and Toshihiro Aono :
Involvement of carbohydrate molecules on zona pellucida in human fertilization,
Journal of Reproductive Immunology, Vol.53, No.1-2, 79-89, 2002.

(要約): Although the involvement of several receptors and ligand molecules in sperm-zona interaction in many species have been proposed, there has been a little analysis of the kinetics between these molecules during the interaction. In the present study, we applied the detection method using surface plasmon resonance (SPR) by a BIAcore apparatus for the analysis of the putative receptor-ligand interaction of sperm-egg binding. Mannose-BSA or [man](5)-[GlcNAc](2)-Asp was immobilized on the surface of a sensor chip. When concanavalin A (Con A) was delivered to each of two different sensor chips to evaluate their usefulness, the resonance signal after sample injection onto a [man](5)-[GlcNAc](2)-Asp-fixed chip decreased rapidly than the mannose-BSA-fixed chip. However, the amount of binding for Con A during the injection onto the [man](5)-[GlcNAc](2)-Asp-fixed chip was high. When acid sperm extracts (acid extracts) and fractions through a CM column, containing protease activity (protease fractions), were delivered to the mannose-BSA-fixed chip, the SPR signal during the injection was not obviously changed compared with that of the control. However, when sperm samples were delivered to the [man](5)-[GlcNAc](2)-Asp-fixed chip, the SPR response during the injection was enormous. These results suggest that the [man](5)-[GlcNAc](2)-Asp-fixed chip is more useful than the mannose-BSA-fixed chip for investigating the interactions with sperm extracts and that the sensitive method using SPR by a BIAcore apparatus is applicable for the analysis of the putative receptor-ligand interaction of sperm-egg binding.
(キーワード): Mannose / Surface plasmon resonance / Sperm-Zona interaction / Lectin-carbohydrate binding
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0165-0378(01)00096-1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11730906; ● Search Scopus @ Elsevier (PMID): 11730906; ● Search Scopus @ Elsevier (DOI): 10.1016/S0165-0378(01)00096-1

(DOI: 10.1016/S0165-0378(01)00096-1, PubMed: 11730906) Song Quan, Shuji Yamano, Koji Nakagawa, Minoru Irahara, Masaharu Kamada and Toshihiro Aono :
Penetrating capacity of human spermatozoa cool preserved in electrolyte-free solution,
The Journal of Reproductive Medicine, Vol.46, No.11, 957-961, 2001.

(要約): To evaluate whether human spermatozoa preserved in electrolyte-free (EF) solution at 4 degrees C possess normal penetrating capacity. The acrosomal status of human spermatozoa cool preserved in EF solution was evaluated before preservation and before and after reinitiation by using chlortetracycline staining. The zona-free hamster egg sperm penetration test was performed using spermatozoa cool preserved in EF solution. The percentages of capacitated and acrosome-reacted spermatozoa cool preserved in EF solution before reinitiation were similar to those of fresh spermatozoa, but they significantly increased after reinitiation. The penetration rate and fertility index of spermatozoa cool preserved in EF solution were comparable to those of fresh spermatozoa (48.3% vs. 50.8% and 1.37 +/- 0.15 vs. 1.29 +/- 0.10, respectively). Human spermatozoa cool preserved in EF solution for one week can possess as much penetrating capacity as fresh spermatozoa.
(キーワード): Acrosome / Adult / Animals / Cell Survival / Cold Temperature / Cricetinae / Electrolytes / Female / Fertilization / Humans / Male / Organ Preservation Solutions / Refrigeration / Semen Preservation / Sperm-Ovum Interactions / Spermatozoa / Time Factors
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11762151; ● Search Scopus @ Elsevier (PMID): 11762151

(PubMed: 11762151) Minoru Irahara, Hirokazu Uemura, Toshiyuki Yasui, Hiromi Kinoshita, Masayo Yamada, Michiko Tezuka, Machiko Kiyokawa, Masaharu Kamada and Toshihiro Aono :
Efficacy of every-other-day administration of conjugated equine estrogen and medroxyprogesterone acetate on gonadotropin-releasing hormone agonists treatment in women with endometriosis,
Gynecologic and Obstetric Investigation, Vol.52, No.4, 217-222, 2001.

(要約): We performed a randomized controlled study to determine the efficacy of add-backed therapy by every-other-day administration of 0.625 mg conjugated equine estrogen (CEE) and 2.5 mg medroxyprogesterone acetate (MPA) on GnRH agonists (GnRH-a) treatment in Japanese women with symptomatic endometriosis. At the end of treatment, serum estrone and estradiol levels in the add-back group (n = 11) were significantly higher than those in the control group (n = 10). The assessment of Beecham classification by bimanual examination, serum CA-125 levels, and the frequency of genital bleeding revealed no significant differences between the two groups. The add-back group showed reduced Kupperman indices relative to those of the control group, and could prevent the loss of bone density. These findings led to a conclusion that GnRH-a therapy added back by every-other-day administration of 0.625 mg CEE and 2.5 mg MPA was a safe and effective treatment for Japanese women with endometriosis.
(キーワード): GnRH agonist / Add-back therapy / Endometriosis
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000052978
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11729332; ● Search Scopus @ Elsevier (PMID): 11729332; ● Search Scopus @ Elsevier (DOI): 10.1159/000052978

(DOI: 10.1159/000052978, PubMed: 11729332) Masahiko Maegawa, Masaharu Kamada, Minoru Irahara, Satoshi Yamamoto, Shuji Yamano, Y Ohmoto, H Gima, C J Thaler and Toshihiro Aono :
Concentration of Granulocyte Elastase in Seminal Plasma is not Associated with Sperm Motility,
Archives of Andrology, Vol.47, No.1, 31-36, 2001.

(要約): Although elastase could affect sperm motility in vitro, secretory leukocytes protease inhibitor (SLPI) prevents sperm from being attacked by elastase. The authors investigated the correlations of elastase level with sperm motility and SLPI level in vivo. Semen samples (n = 116) were collected and centrifuged after semen analysis. Elastase and SLPI levels were determined by an enzyme immunosorbent assay. Samples were classified by elastase levels into low (<250 ng/mL), moderate (250-1,000 ng/mL), and high elastase groups (> or =1,000 ng/mL). Elastase levels (range, 2.8-23,974.4 ng/mL) were not associated with sperm motility. The median SLPI level in the high elastase group was 15,900 ng/mL (range, 2.860-46,900 ng/mL). However, there was no significant correlation between elastase and SLPI levels in seminal plasma. Since SLPI forms a 1:1 complex with elastase, these results suggest that seminal plasma has a sufficient amount of SLPI to protect spermatozoa from elastase.
(キーワード): granulocyte elastase / secretory leukocyte protease inhibitor / seminal plasma / sperm motility
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11442333; ● Search Scopus @ Elsevier (PMID): 11442333

(PubMed: 11442333) Keizo Deguchi, Masaharu Kamada, Minoru Irahara, Masahiko Maegawa, Satoshi Yamamoto, Yasukazu Ohmoto, Kaoru Murata, Toshiyuki Yasui, Shuji Yamano and Toshihiro Aono :
Postmenopausal changes in production of type 1 and type 2 cytokines and the effects of hormone replacement therapy,
Menopause, Vol.8, No.4, 266-273, 2001.

(要約): An appropriate defense against infective agents or malignant cells is attributed to the exquisitely balanced T helper 1 type (cellular) and T helper 2 type (humoral) immune reactions. We investigated the effect of hormone replacement therapy (HRT) on postmenopausal changes in the production of interferon (IFN)-gamma and interleukin (IL)-10, a type 1 and a type 2 cytokine, respectively. Both cytokines were measured by ELISA in the supernatant of lipopolysaccharide-stimulated whole blood cells from 72 untreated and 44 HRT-treated women. Thirteen women were examined before and during HRT. The production of IFN-gamma in women in their 40s and in postmenopausal women was significantly higher compared with that of younger women. However, IFN-gamma fell to the lowest level in the late postmenopausal stage, whereas the production of IL-10 increased gradually with age and in parallel with the postmenopausal period. Thus, in women in the mid-and late postmenopausal period, excessive production of type 2 cytokine (IL-10) compared with type 1 cytokine (IFN-gamma) occurred. The IFN-gamma levels of women on HRT were significantly lower than those of untreated women in the early and mid-postmenopausal stages, and IL-10 levels of women on HRT were significantly lower than those of untreated women in the mid-and late postmenopausal stages. HRT induced a significant decrease in the production of IL-10 and tended to lower the level of IFN-gamma. Production of IL-10 is augmented in postmenopausal women. HRT probably prevents postmenopausal women from an aberration of the immune system by improving the balance of type 1 and type 2 immune reactions.
(キーワード): Menopause / IL-10 / IFN-γ / Immunosupperssion / Cellular immunity / Humoral immunity
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11449084; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034946347

(PubMed: 11449084, Elsevier: Scopus) Koji Ogura, Minoru Irahara, Machiko Kiyokawa, Michiko Tezuka, Toshiya Matsuzaki, Toshiyuki Yasui, Masaharu Kamada and Toshihiro Aono :
Effects of leptin on secretion of LH and FSH from primary cultured female rat pituitary cells,
European Journal of Endocrinology, Vol.144, No.6, 653-658, 2001.

(要約): Leptin, which is the product of the obese gene, is believed to play important roles in pubertal development and reproductive function in females. In a study using adult male rats, it was found that leptin stimulated secretion of gonadotropin from the pituitary in a dose-related manner. However, there has been no such study in female rats. To investigate the effects of leptin on the production of LH and FSH from the pituitary in female rats, using primary cultured pituitary cells. In this study, we determined body weight, serum leptin concentration and serum estradiol (E(2)) concentration in female Wistar rats at 3, 5, 6, 7, 9 and 11 weeks of age, and cultured pituitary cells from 6-week-old female Wistar rats with leptin (0--10(-7) mol/l) and GnRH (0 or 10(-8) mol/l). Then basal and GnRH-stimulated extra- and intracellular LH and FSH were assayed by RIA. Serum leptin concentration increased with increases in body weight and E(2) concentration. The pubertal serum leptin concentration was about 10(-10) mol/l. At a lower or moderate concentration, leptin produced dose-related increases in both basal and GnRH-stimulated extra- and intracellular LH and FSH in pituitary cells. At a concentration of 10 mol/l, leptin significantly (P<0.05) stimulated both basal and GnRH-stimulated extra- and intracellular LH and FSH. However, at greater concentrations, these effects diminished. These results indicated that leptin induced pituitary cells to produce and secrete both LH and FSH, with or without GnRH. The concentration of leptin that induced the greatest production of gonadotropins by pituitary cells was 10(-10) mol/l, which was the same as the physiological pubertal concentration. Leptin may be involved in the onset of puberty. It is also conceivable that leptin may be a cause of ovulatory failure, not only in weight loss but also in weight gain.
(キーワード): Animals / Body Weight / Cells, Cultured / Estradiol / Extracellular Space / Female / Follicle Stimulating Hormone / Gonadotropin-Releasing Hormone / Leptin / Luteinizing Hormone / Pituitary Gland / Rats
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11375800; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034981667

(PubMed: 11375800, Elsevier: Scopus) Masaya Takikawa, Masaharu Kamada, Masahiko Maegawa, Shuji Yamano, Minoru Irahara, Toshihiro Aono, Siroh Futaki, Yasukazu Ohmoto and Samuel S Koide :
Evaluation of two sperm antigens,rSMP-B and YWK-||,as targets for immunocontraception,
Zygote, Vol.9, No.2, 145-151, 2001.

(要約): To determine whether sperm membrane components, rSMP-B and YWK-II, are suitable candidates as immunocontraceptives in humans, antifertility activities of the antibodies to the peptide fragments, rSMP-229 and rSMP-230 of rSMP-B and YAL-198 of YWK-II, were examined. In a previous report, anti-rSMP-230 antibody was shown to immobilise human sperm and to block human fertilisation, and the antigen (rSMP-230) to interact with antisperm antibodies found in sera of infertile women. Antibody to the second synthetic peptide, rSMP-229, corresponding to a different segment of rSMP-B, mimicked the biological activities of the anti-rSMP-230 antibody. Anti-YAL-198 antibody significantly, although weakly, inhibited human fertilisation. In the murine model, the anti-rSMP-B antibodies blocked in vitro fertilisation of mouse eggs but had no influence on embryo growth. Anti-YAL-198 antibody, however, arrested the growth of zygotes. In conclusion, rSMP-B, a human sperm protein, is a promising candidate in the development of an immunocontraceptive for human application. A second sperm protein, YWK-II, is effective as an antifertility immunogen in experimental animals.
(キーワード): Antisperm antibody / Contraception / 胚発生 (embryonic growth) / Fertilisation / Human sperm protein
(出版サイトへのリンク): ● Publication site (DOI): 10.1017/S0967199401001150
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11358321; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035348924

(DOI: 10.1017/S0967199401001150, PubMed: 11358321, Elsevier: Scopus) 安井敏之, 手束典子, 山田正代, 上村浩一, 苛原稔, 青野敏博, 三浦雅一 :
ホルモン補充療法中の骨密度の増加にエストロンは関与するか?,
Osteoporosis Japan, Vol.9, No.2, 217-218, 2001年. 上村浩一, 手束典子, 山田正代, 安井敏之, 苛原稔, 青野敏博 :
妊娠·産褥期の血中Osteoprotegerin(OPG)/Osteoclastgenesis Inhibitory Factor(OCIF)濃度の推移及び骨代謝との関係の検討 Osteoporosis Japan,
Osteoporosis Japan, Vol.9, No.2, 174-176, 2001年. Takeshi Katou, Hiroyuki Furumoto, Tomoko Ogura, Yasuyo Onishi, Minoru Irahara, Shuji Yamano, Masaharu Kamada and Toshihiro Aono :
Expression of IL-17 mRNA in Ovarian Cancer,
Biochemical and Biophysical Research Communications, Vol.282, No.3, 735-738, 2001.

(要約): IL-17 is considered as a proinflammatory cytokine. We have demonstrated IL-17 is an angiogenic factor and promotes tumor growth in murine tumor models. In this report, we investigated the expression of IL-17 mRNA by RT-PCR and the relationship between IL-17 expression and microvascular density in ovarian cancer. IL-17 mRNA was expressed in 11 (64.7%) of 17 ovarian cancer. And the average number of blood vessels observed in IL-17 positive tumors (173.4 +/- 55.1/mm(2)) was significantly higher than that in negative tumors (107.7 +/- 57.8/mm(2)). These results indicated IL-17 is expressed in a considerable proportion of ovarian cancer and promotes tumor angiogenesis. There was no significant relationship between IL-17 expression and clinicopathologic parameters.
(キーワード): Base Sequence / DNA Primers / Female / Gene Expression Regulation, Neoplastic / Humans / Interleukin-17 / Microcirculation / Neovascularization, Pathologic / Ovarian Neoplasms / RNA, Messenger / RNA, Neoplasm / Reverse Transcriptase Polymerase Chain Reaction
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.2001.4618
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11401524; ● Search Scopus @ Elsevier (PMID): 11401524; ● Search Scopus @ Elsevier (DOI): 10.1006/bbrc.2001.4618

(DOI: 10.1006/bbrc.2001.4618, PubMed: 11401524) Miho Senuma, Shuji Yamano, Koji Nakagawa, Minoru Irahara, Masaharu Kamada and Toshihiro Aono :
Progesterone accelerates the onset of capacitation in mouse sperm via T-type calcium channels,
Archives of Andrology, Vol.47, No.2, 127-134, 2001.

(要約): This study was undertaken to evaluate whether progesterone induces capacitation of mouse spermatozoa. When sperm were evaluated by chlortetracycline staining, addition of progesterone significantly increased the proportion of spermatozoa exhibiting the B pattern at 60 minutes of incubation, compared with that before incubation (23 +/- 6.2% vs. 13 +/- 2.9%, p < 0.01) and that in hTF medium without progesterone (23 +/- 6.2% vs. 13 +/- 4.2%, p < 0.01). If the redistribution of proteins in sperm plasma membrane such as protein binding calcium ion were defined as capacitation, it could be said that progesterone promoted capacitation of mouse sperm. This progesterone-induced capacitation was prevented by depletion of extracellular calcium ion and addition of NiCl2, a T-type calcium channel blocker, although thapsigargin, an inhibitor of Ca2+-ATPase, did not increase the number of capacitated sperm (B pattern; progesterone vs. progesterone + depletion of calcium ion, 18 +/- 3.5% vs. 8 +/- 2.5%, p < 0.05, progesterone vs. progesterone + NiCl2, 20 +/- 3.8% vs. 6 +/- 5.2%, p < 01). Furthermore, genistein, a protein tyrosine phosphorylation inhibitor, inhibited progesterone-induced capacitation (B pattern; progesterone vs. progesterone + genistein, 20 +/- 3.8% vs. 11 +/- 2.4%, p < 01). In conclusion, progesterone induces capacitation in mouse sperm and this capacitation may be associated with calcium influx and tyrosine phosphorylation.
(キーワード): Animals / Calcium Channels, T-Type / Enzyme Inhibitors / Male / Mice / Mice, Inbred ICR / Progesterone / Protein-Tyrosine Kinases / Sperm Capacitation / Spermatozoa
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11554684; ● Search Scopus @ Elsevier (PMID): 11554684

(PubMed: 11554684) Masaharu Kamada, M Takikawa, Masahiko Maegawa, Satoshi Yamamoto, Shuji Yamano, Minoru Irahara and Toshihiro Aono :
Specific Cytotoxicity of Antibody to YAL-198, a Sperm Antigen Peptide, to Murine Zygote,
Archives of Andrology, Vol.47, No.2, 89-96, 2001.

(要約): Active immunization with the peptide segments rSMP-230 and YAL-198, corresponding to the hydrophilic extracellular domain of two human sperm antigens (rSMP-B and YWK-II, respectively), reduced fertility in female rats by different mechanisms. The anti-rSMP-230 antibody interferes with human and murine fertilization, and the anti-YAL-198 antibody blocks the development of mouse embryos. The authors examined in vitro at which stage the antibodies to rSMP-230 and YAL-198 were cytotoxic to murine embryos up to morula/blastocyst stage. Anti-rSMP-230 antibody was not cytotoxic to any stages. On the other hand, the anti-YAL-198 antibody arrested the growth of embryos at the 2-cell stage but not at more advanced developmental stages. When the anti-YAL-198 antibody was used, spotty staining was observed only on the surfaces of embryos that had arrested at the 2-cell stage. Unstained embryos, however, continued to develop normally. In contrast, the anti-rSMP-230 antibody stained murine sperm but failed to stain murine ova and embryos. The present results suggest that the human sperm components rSMP-B and YWK-II play important roles in sperm-egg interaction and early development of the embryo, respectively.
(キーワード): antibody / antigen / cytotoxicity / murine / sperm
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11554689; ● Search Scopus @ Elsevier (PMID): 11554689

(PubMed: 11554689) K Myogo, Shuji Yamano, Koji Nakagawa, Masaharu Kamada, Masahiko Maegawa, Minoru Irahara and Toshihiro Aono :
Sperm-Immobilizing Antibodies Block Capacitation in Human Spermatozoa,
Archives of Andrology, Vol.47, No.2, 135-142, 2001.

(要約): Sperm-immobilizing antibodies block human fertilization by interfering with the acrosome reaction (AR). To clarify the mechanism of blockage of AR by sperm-immobilizing antibodies, the authors examined their effects on the increase of intracellular free Ca2+ concentration induced by follicular fluids (Ca2+ influx) in spermatozoa and on their capacitation. Sperm-immobilizing antibodies did not suppress Ca2+ influx induced by follicular fluid, but they inhibited capacitation of human spermatozoa. Namely delta%AR (%AR after addition of an AR inducer--%AR before treatment) induced by progesterone was significantly (p < .0001) lower when spermatozoa were incubated in human tubal fluid medium cotaining antibody-positive serum (1.2%), compared to that when incubated in control medium (19.2%). Furthermore, the proportion of both spermatozoa that became capacitated and ones that had become capacitated decreased significantly (p < .0001) after 2, 4, and 6 h of incubation in medium containing antisperm antibody-positive serum, compared to those of spermatozoa incubated in control medium. In conclusion, sperm-immobilizing antibodies may be closely related to their blockage of capacitation.
(キーワード): Ca2+ influx / capacitation / CTC / human spermatozoa / sperm-immobilizing antibody
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/014850101316901343
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11554685; ● Search Scopus @ Elsevier (PMID): 11554685; ● Search Scopus @ Elsevier (DOI): 10.1080/014850101316901343

(DOI: 10.1080/014850101316901343, PubMed: 11554685) Minoru Irahara, Takao Tamura, Toshiya Matsuzaki, Seiichiro Saito, Toshiyuki Yasui, Shuji Yamano, Masaharu Kamada and Toshihiro Aono :
Orexin-A Suppresses the Pulsatile Secretion of Luteinizing Hormone via β-Endorphin,
Biochemical and Biophysical Research Communications, Vol.281, No.1, 232-236, 2001.

(要約): Orexins, the novel hypothalamic neuropeptides that stimulate feeding behavior, have been shown to suppress the pulsatile secretion of LH in ovariectomized rats. However, the mechanism of this action is still not clear. We examined the effect of naloxone, a specific opioid antagonist, on the suppression of the pulsatile secretion of LH by orexins to determine whether beta-endorphin is involved in this suppressive effect. We administered orexins intracerebroventricularly and injected naloxone intravenously in ovariectomized rats, and we measured the serum LH concentration to analyze the pulsatile secretion. Administration of orexin-A significantly reduced the mean LH concentration and the pulse frequency, but coadministration of naloxone significantly restored the mean LH concentration and the pulse frequency. Administration of orexin-B also significantly reduced the mean LH concentration and the pulse frequency, and coadministration of naloxone did not restore them. These results indicate that orexin-A, but not orexin-B, suppresses GnRH secretion via beta-endorphin.
(キーワード): Animals / Carrier Proteins / Dose-Response Relationship, Drug / Endorphins / Female / Injections, Intravenous / Intracellular Signaling Peptides and Proteins / Luteinizing Hormone / Naloxone / Narcotic Antagonists / Neuropeptides / Radioimmunoassay / Rats / Rats, Wistar / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.2001.4328
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11178985; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034810687

(DOI: 10.1006/bbrc.2001.4328, PubMed: 11178985, Elsevier: Scopus) Masaharu Kamada, Minoru Irahara, Masahiko Maegawa, Yasukazu Ohmoto, Takashi Takeji, Toshiyuki Yasui and Toshihiro Aono :
Postmenopausal changes in serum cytokine levels and hormone replacement therapy,
American Journal of Obstetrics and Gynecology, Vol.184, No.3, 309-314, 2001.

(要約): Our purpose was to investigate the effect of hormone replacement therapy on the postmenopausal changes in serum cytokine levels. Fifteen cytokines were measured by an enzyme-linked immunosorbent assay in 97 untreated and hormone replacement-treated women. Thirteen women were examined before and during hormone replacement therapy. Serum concentrations of macrophage colony-stimulating factor were significantly (P < .05) lower during the early postmenopausal period (< or = 10 years) than the values in premenopause and the elevated levels in the late postmenopausal period (< or = 30 years). A significant increase in tumor necrosis factor alpha and a decline in transforming growth factor beta1 were found in late postmenopausal women. Serum levels of macrophage colony-stimulating factor in women receiving hormone replacement therapy were significantly higher than those in untreated postmenopausal women. Furthermore, hormone replacement therapy induced a significant (P < .01) increase in serum levels of macrophage colony-stimulating factor, whereas serum levels of other cytokines were not affected. It is well documented that macrophage colony-stimulating factor lowers serum cholesterol concentrations and prevents atherosclerosis. Inducing the production of macrophage colony-stimulating factor is a possible additional mechanism of hormone replacement therapy in mediating the antiatherogenic effect.
(キーワード): Atherosclerosis / estrogen / macrophage colony-stimulating factor / menopause
(出版サイトへのリンク): ● Publication site (DOI): 10.1067/mob.2001.109940
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11228479; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035094158

(DOI: 10.1067/mob.2001.109940, PubMed: 11228479, Elsevier: Scopus) Masaharu Kamada, Minoru Irahara, Masahiko Maegawa, Toshiyuki Yasui, Shuji Yamano, Masayo Yamada, Mitiko Tezuka, Yuka Kasai, Keiichi Deguchi, Yasukazu Ohmoto and Toshihiro Aono :
B cell subsets in postmenopausal women and the effect of hormone replacement therapy,
Maturitas, Vol.37, No.3, 173-180, 2001.

(要約): In elderly subjects the capacity for antibody production is depressed. This immunosenescence state of humoral immunity is associated with the occurrence of autoimmune disorders involving CD5+ B (B-1) cells. Since estrogen is capable of stimulating the production of autoantibodies, this sex steroid hormone may be a contributing cause of the higher incidence of autoimmune diseases in women. In the present study, B cell subsets in women during the postmenopausal period was determined. The effect of hormone replacement therapy (HRT) on B cell subsets was examined to establish whether the administration of gonadal hormones influence humoral immunity in postmenopausal women. Forty six untreated pre- and postmenopausal women and 39 women on HRT were studied. The proportion of B-1 (CD5+) and conventional CD5- B (B-2) lymphocytes was determined by two-color flow cytometry. Serum autoantibodies to a nuclear antigen and to interleukin (IL)-1alpha were measured by immunofluorescence and by radioimmunoassay, respectively. Thirteen women were examined prospectively before and during HRT. In late postmenopausal women (> or = 30 years postmenopausal period), the proportion of B-2 cells was significantly reduced (p<0.01) compared to those of premenopausal and perimenopausal women. HRT induced a significant (p<0.01) increase in the percentage of B-2 cells, while that of B-1 cells remained unchanged. HRT did not affect autoantibody production. HRT may retard the progress of immunosenescence by increasing the production of B-2 cells. Moreover, HRT appears not to increase the risk of autoimmune diseases developing in postmenopausal women.
(キーワード): CD5+B cell / B-2 cell / B-1 cell / Autoimmune disease
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0378-5122(00)00180-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11173179; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035977616

(DOI: 10.1016/S0378-5122(00)00180-8, PubMed: 11173179, Elsevier: Scopus) Hiromi Kinoshita, Toshiyuki Yasui, Kenjiro Ushigoe, Minoru Irahara, Minoru Tanaka, Kunio Nakashima and Toshihiro Aono :
Expression of Ovarian Prolactin Receptor in Relation to Hormonal Changes during Induction of Ovulation in the Rat,
Gynecologic and Obstetric Investigation, Vol.52, No.2, 132-138, 2001.

(要約): We examined the relationships between the expression of the short and long forms of the prolactin (PRL) receptor (PRLR) mRNA in the ovary and changes in the levels of serum hormones such as sex steroid hormones and PRL during induction of ovulation in the rat. The expression of both forms of PRLR mRNA in the ovary was examined by Northern blot analysis in immature female rats treated with pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Ovarian tissues and blood samples were obtained before treatment, 24 and 48 h after PMSG injection and 4, 6, 8, 12, 24 and 48 h after hCG treatment. Serum levels of 17beta-estradiol, progesterone and PRL were determined by radioimmunoassay. Serum levels of 17beta-estradiol rapidly increased to a maximal level 48 h after PMSG injection and then rapidly declined until 4 h after hCG injection. Serum levels of progesterone gradually increased after PMSG treatment, markedly increased to 114.2 nmol/l 8 h after hCG treatment and remained high until 48 h after hCG treatment. The serum level of PRL peaked at 66.2 microg/l (p < 0.01) 48 h after PMSG injection, and a temporary decrease after hCG treatment was followed by a continuously high level from 8 to 48 h. The expression of the long form of PRLR mRNA increased significantly (p < 0.01) to 688% of the control level after PMSG treatment, while that of the short form increased to only 184% of the control level. The expression of the long form of PRLR-mRNA rapidly declined until 6 h and then gradually increased until 48 h after hCG treatment. On the other hand, the expression of the short form of PRLR mRNA decreased to a nadir 12 h after hCG injection and then increased significantly (p < 0.01) to 142% of the control level. Our results showed that the changes in the short and long forms of PRLR mRNA differed in a time-specific manner and that these two forms are involved in different functions in the rat ovary during induction of ovulation. It is thought that the long form of PRLR mRNA is involved in folliculogenesis, while the short form of PRLR mRNA may play an important role in the formation and maintenance of the corpus luteum in the rat ovulatory cycle.
(キーワード): Prolactin / Prolactin receptor / Overy / Induction of ovulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000052958
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11586043; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035742710

(DOI: 10.1159/000052958, PubMed: 11586043, Elsevier: Scopus) Masaharu Kamada, Minoru Irahara, Masahiko Maegawa, Yasukazu Ohmoto, Kaoru Murata, Toshiyuki Yasui, Shuji Yamano and Toshihiro Aono :
Transient Increase in the Levels of T-Helper 1 Cytokines in Postmenopausal Women and the Effects of Hormone Replacement Therapy,
Gynecologic and Obstetric Investigation, Vol.52, No.2, 82-88, 2001.

(要約): The aim of this study was to determine, at least in part, T-cell function in postmenopausal women and the effects of hormone replacement therapy (HRT). Levels of T-helper 1 (Th1) cytokines (IL-2 and IFN-gamma) and T-helper 2 (Th2) cytokines (IL-4 and IL-10) produced by phytohemagglutinin-stimulated whole blood cells from 72 untreated and 44 HRT-treated women were measured by ELISA. Thirteen of the 44 HRT-treated women were examined before and during HRT. The production of IL-2 increased gradually with advance of the postmenopausal period. The levels of IL-2 in women in the early (< or =10 years) and mid (>10 and <30 years) postmenopausal stages were significantly higher than those in women in their second, third and fourth decades. The level in women in the late (> or =30 years) postmenopausal stage, however, was significantly lower than those in women in the early and mid postmenopausal stages. The level of IFN-gamma was highest in women in the mid postmenopausal stage. On the other hand, the levels of Th2 cytokines did not change with age or after menopause until the mid postmenopausal period but were significantly lower in women in the late postmenopausal stage. IFN-gamma levels in women on HRT were significantly lower than those in untreated postmenopausal women at all postmenopausal stages. HRT induced a significant decrease in the production of IL-2 and IL-4. In conclusion, production of Th1 cytokines is augmented in women after menopause. HRT prevents this increase, thereby improving the aberration of Th1/Th2 balance that is implicated in an inadequate immune response and pathological conditions.
(キーワード): Menopause / IL-2·IFN-γ / Th1 cytokine / Th2 cytokine
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11586033; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035741848

(PubMed: 11586033, Elsevier: Scopus) Toshiyuki Yasui, Hirokazu Uemura, Michiko Tezuka, Masayo Yamada, Minoru Irahara, Masakazu Miura and Toshihiro Aono :
Biological Effects of Hormone Replacement Therapy in Relation to Serum Estradiol Levels,
Hormone Research, Vol.56, No.1-2, 38-44, 2001.

(要約): Tissues in various parts of the body have different sensitivities to estradiol. However, it is very difficult to measure the serum estradiol levels precisely in women receiving oral conjugated equine estrogen, which is a mixture of estrogens. In the present study, we precisely measured the serum levels of estradiol in postmenopausal women undergoing hormone replacement therapy (HRT), and we clarified the relationships between serum estradiol levels and the effects of HRT on the Kupperman index, bone mineral density (BMD), serum gonadotropin, lipid metabolism and unscheduled bleeding as the clinical endpoints. Sixty-eight postmenopausal or bilaterally ovariectomized women, aged 30-64 years, who had been suffering from vasomotor symptoms such as hot flush or atrophy of the vagina were randomly assigned to two groups: one group of 34 patients who received oral administration of 0.625 mg conjugated equine estrogen (CEE, Premarin, Wyeth) and 2.5 mg medroxyprogesterone acetate (MPA, Provera, Upjohn) every other day, and another group of 34 patients who received oral administration of 0.625 mg CEE and 2.5 mg MPA every day. All subjects were re-classified into three groups according to the serum estradiol level after 12 months of treatment: (1) low estradiol group (<15 pg/ml, n = 25); (2) middle estradiol group (> or =15 and <25 pg/ml, n = 27), and (3) high estradiol group (> or =25 pg/ml, n = 16). We examined the relationships between serum estradiol level and the effects of estradiol on the Kupperman index, BMD, serum gonadotropin levels, lipid profile and unscheduled bleeding in these three groups. RESULTS obtained by using our newly developed high-performance liquid chromatography (HPLC)-radioimmunoassay (RIA) system clearly showed that the effects on each tissue in postmenopausal women receiving oral CEE and MPA is closely related to estradiol level. The effects of HRT on BMD, serum gonadotropin levels and lipid profile were shown to be clearly dependent on the serum estradiol levels, while the effect of HRT on the Kupperman index was independent of the serum estradiol level. Furthermore, it was also found that a very low concentration of estradiol (<15 pg/ml) was sufficient to suppress the serum LH and FSH levels and to relieve vasomotor symptoms, and that the minimum concentration of estradiol required to increase BMD was 15 pg/ml. On the other hand, the level of estradiol required to reduce total cholesterol, low density lipoprotein-cholesterol (LDL-C) and apolipoprotein B (Apo B) was found to be more than 25 pg/ml, while the level required to increase high density lipoprotein-cholesterol (HDL-C) and apolipoprotein A1 (Apo A1) was at least 15 pg/ml. The incidence of unscheduled bleeding was also lower in the low estradiol group than in the other estradiol level groups. These results suggest that the different clinical endpoints have different response thresholds and thus reflect tissue sensitivity to estradiol levels achieved by HRT.
(キーワード): Hormone replacement therapy / Estradiol / Bone / Lipid / Gonadotropin
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000048088
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11815726; ● Summary page in Scopus @ Elsevier: 2-s2.0-0035707994

(DOI: 10.1159/000048088, PubMed: 11815726, Elsevier: Scopus) Shuji Yoshikawa, Masaharu Kamada, Masahiko Maegawa, Satoshi Yamamoto, Minoru Irahara, Shuji Yamano, Toshihiro Aono, Hiroshi Kido and Samuel S Koide :
Hormonal Control of mRNA Expression of Immunoglobulin Binding Factor in Uterine Cervix,
Biochemical and Biophysical Research Communications, Vol.279, No.3, 898-903, 2000.

(要約): Uterine cervical mucus contains an immunoglobulin binding factor (IgBF). It may play a role in preventing antibody production against sperm in the female reproductive tract. To elucidate the mechanism involved in the production of activated IgBF, we determined the effects of hormones on the expression of mRNAs of IgBF and of protein disulfide isomerase (PDI), activating enzyme, in uterine cervix by quantitative RT-PCR. The uterine cervices of female rats were excised at preovulatory, ovulatory, and postovulatory phases. The human uterine cervical adenocarcinoma cells (TCO-2) were cultured for 24 h in serum-free medium containing 17beta-estradiol or progesterone. Expression of IgBF and PDI mRNAs was significantly highest during the ovulatory phase. 17beta-estradiol stimulated the expression of both mRNAs in TCO-2; whereas progesterone was ineffective. In conclusion, estrogen regulates the production of IgBF by the endocervix and PDI in vivo, thereby increasing the level of activated IgBF in the female reproductive tract during the ovulatory phase, allowing sperm to enter the uterine cavity.
(キーワード): Animals / Cervix Uteri / Estradiol / Female / Gene Expression Regulation / Humans / In Situ Hybridization / Lymphokines / Progesterone / Prostatic Secretory Proteins / Protein Disulfide-Isomerases / RNA, Messenger / Rats / Reverse Transcriptase Polymerase Chain Reaction / Tumor Cells, Cultured
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.2000.4036
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11162446; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034731493

(DOI: 10.1006/bbrc.2000.4036, PubMed: 11162446, Elsevier: Scopus) Minoru Irahara, Toshiyuki Yasui, Michiko Tezuka, Kenjiro Ushigoe, Shuji Yamano, Masahiko Kamada and Toshihiro Aono :
Evidence that Toki-shakuyaku-san and its Ingredients Enhance the Secretion of a Cytokine-Induced Neutrophil Chemoattractant (CINC/gro) in the Ovulatory Process,
Methods and Findings in Experimental and Clinical Pharmacology, Vol.22, No.10, 725-730, 2000.

(要約): We investigated the effects of Toki-shakuyaku-san and its crude ingredients in relation to the secretion of a cytokine-induced neutrophil chemoattractant, CINC/gro, interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF alpha) in the ovulatory process. Toki-shakuyaku-san significantly (p < 0.01) stimulated the secretion of 17 beta-estradiol but did not stimulate the secretion of progesterone in cultured whole ovarian dispersates. Toki-shakuyaku-san enhanced the secretion of CINC/gro in a dose-dependent manner and the production of CINC/gro at concentrations of 10 and 100 micrograms/ml of Toki-shakuyaku-san increased significantly (p < 0.01). Toki-shakuyaku-san also enhanced secretions of both IL-1 beta and TNF alpha, which are known to stimulate the secretion of CINC/gro in the ovulatory process. The production of TNF alpha increased significantly (p < 0.05) with 10 and 100 micrograms/ml of Toki-shakuyaku-san. Atractylodis Lanceae Rhizoma, Cnidii Rhizoma, Angelicae Radix, Paeoniae Radix and Alismatis Rhizoma, which are crude ingredients of Toki-shakuyaku-san, significantly (p < 0.01) enhanced the secretion of CINC/gro at concentrations of 100 micrograms/ml. The results of this study show that Toki-shakuyaku-san can stimulate the secretion of 17 beta-estradiol and stimulate the ovulatory process by stimulating the production of CINC/gro, IL-1 beta and TNF alpha in vitro. As a treatment for ovulatory disorders, Toki-shakuyaku-san may have stimulatory effects on both steroidogenesis and the ovulatory process.
(キーワード): Cytokine / induced neutrophil chemoattractant / Interleukin / 1β / Ovulation / Toki-shakuyaku-san / Tumornecrosis factor α
(出版サイトへのリンク): ● Publication site (DOI): 10.1358/mf.2000.22.10.802288
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11346892; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034456929

(DOI: 10.1358/mf.2000.22.10.802288, PubMed: 11346892, Elsevier: Scopus) Masaharu Kamada, Minoru Irahara, Masahiko Maegawa, Toshiyuki Yasui, Takashi Takeji, Masayo Yamada, Mitiko Tezuka, Yuka Kasai and Toshihiro Aono :
Effect of hormone replacement therapy on post-menopausal changes of lymphocytes and T cell subests,
Journal of Endocrinological Investigation, Vol.23, No.6, 376-382, 2000.

(要約): Immunosenescence is associated with the occurrence of lethal diseases, such as infection and malignancy. Since endocrinosenescence occurs simultaneously with immunosenescence, we determined whether or not lymphocytes and T cell subsets were altered in post-menopausal women. The ability of hormone replacement therapy (HRT) to reverse or modify the aberrations of the cell populations observed in elderly women was also examined. Thirty-nine untreated post-menopausal women and 39 women on HRT were studied. The proportions of lymphocytes and T cell subsets (helper, cytotoxic and immature T cells, and naive and memory/activated T cells) were determined by two color flow cytometry. Thirteen women were examined before and during HRT. At late post-menopause (> or = 30 years post-menopausal period), the proportion of peripheral blood lymphocytes showed a tendency to decline (p=0.06) compared with that at early (< or = 10 years) post-menopause. Significant (p<0.05) decrease in naive T cells and an increase in memory/activated T cells occurred at late post-menopause compared to those at early post-menopause. The percentage of lymphocytes in women on HRT was significantly (p<0.05) higher than that in untreated women at late post-menopausal stage. Furthermore, in a prospective study, HRT induced a significant (p<0.02) increase in the percentage of lymphocytes but showed no effect on the aberrations of naive and memory/activated T cells. HRT prevents the decline in the lymphocytes observed in post-menopausal women. However, HRT appears not to influence the observed alteration in T cell subsets.
(キーワード): Menopause / immunosenescence / naive T cell / memory T cell / estrogen deficiency / aging
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10908165; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033932862

(PubMed: 10908165, Elsevier: Scopus) Noriko Tamura, Minoru Irahara, Akira Kuwahara, Kenjiro Ushigoe, Hiromu Sugino and Toshihiro Aono :
Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells,
European Journal of Endocrinology, Vol.142, No.5, 506-511, 2000.

(要約): To evaluate the effect of the growth factor activin A on the secretion of prolactin (PRL) and GH in cultured GH3 cells. The concentrations of PRL and GH secreted from GH3 cells cultured in media with and without activin A were measured by RIA, and the expression of PRL mRNA and GH mRNA were analyzed using the Northern blot method. Activin A significantly inhibited PRL release from GH3 cells cultured for 48h in a dose-dependent manner (activin: 0.3-3nM). The inhibitory effects of 3nM activin A were observed in the culture from 12h to 48h (53.2% of control). Activin A (3nM) also significantly inhibited the expression of PRL mRNA at 24h (33.8% of control). In contrast, activin A significantly stimulated GH release from GH3 cells cultured for 48h in a dose-dependent manner (activin: 0.3-3nM). The stimulatory effect of 3nM activin A was observed in the culture for 48h (157.6% of control). Activin A (3nM) also significantly stimulated the expression of GH mRNA at 24h (183.6% of control). In spite of these significant changes in PRL and GH secretion, pit-1 mRNA levels were not significantly changed by activin A. These findings indicated that activin A modulates PRL and GH secretion through the regulation of PRL and GH gene transcription in GH3 cells, but that these effects are unrelated to pit-1 gene expression.
(キーワード): Activins / Animals / Blotting, Northern / Gene Expression / Growth Hormone / Inhibins / Pituitary Neoplasms / Prolactin / RNA, Messenger / Radioimmunoassay / Rats / Time Factors / Tumor Cells, Cultured
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10802530; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034039057

(PubMed: 10802530, Elsevier: Scopus) Hirokazu Uemura, Minoru Irahara, Naoto Yoneda, Toshiyuki Yasui, Kaori Genjida, Ken-ichi Miyamoto, Toshihiro Aono and Eiji Takeda :
Close correlation between estrogen treatment and renal phosphate reabsorption capacity,
The Journal of Clinical Endocrinology and Metabolism, Vol.85, No.3, 1215-1219, 2000.

(要約): To determine the influence of estrogen on the activity of renal proximal tubular reabsorption of inorganic phosphate (Pi) in women, we examined the changes of the renal threshold phosphate concentration (also denoted as TmP/GFR), as well as the changes in the concentrations of mineral components in the circulation in two groups of women--one receiving hormone replacement therapy (HRT) and one receiving gonadotropin-releasing hormone agonists (GnRH-a) therapy. We also examined the changes in the concentrations of serum PTH in the GnRH-a group. The patients in the HRT group were continuously treated with 0.625 mg conjugated equine estrogens plus 2.5 mg medroxyprogesterone acetate per day. The patients in the GnRH-a group were treated with a monthly injection of 3.75 mg leuprolide acetate depot for 6 months. The values of TmP/GFR decreased in all of the patients who received HRT. The mean percentage change in TmP/GFR was -14.5% (range, -24.3% to -9.6%). In contrast, in all of the patients treated with GnRH-a, the values of TmP/GFR increased after 6 months of treatment (the mean percentage change was 28.5%; range, 18.2-78.3%) and returned to the preadministration level at 12 weeks after stopping therapy. In these patients, both the values of TmP/GFR and the concentrations of serum Pi correlated significantly with circulating estradiol levels (r = -0.767, P < 0.01 and r = -0.797, P < 0.01, respectively), but the concentrations of serum corrected calcium did not correlate. Moreover, in the same patients, the levels of serum intact PTH decreased significantly (P < 0.05) after 6 months of treatment, but at 12 weeks after stopping therapy the trends of these levels varied among individual patients. These results suggest that estrogen could act directly to suppress sodium-dependent Pi reabsorption in the renal proximal tubules.
(キーワード): Adult / Bone Density / Climacteric / Endometriosis / Estradiol / Estrogen Replacement Therapy / Female / Glomerular Filtration Rate / Gonadotropin-Releasing Hormone / Humans / Hysterectomy / Kidney / Middle Aged / Parathyroid Hormone / Phosphates
(出版サイトへのリンク): ● Publication site (DOI): 10.1210/jcem.85.3.6456
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10720065; ● Search Scopus @ Elsevier (PMID): 10720065; ● Search Scopus @ Elsevier (DOI): 10.1210/jcem.85.3.6456

(DOI: 10.1210/jcem.85.3.6456, PubMed: 10720065) 安井敏之, 手束典子, 山田正代, 上村浩一, 苛原稔, 青野敏博, 三浦雅一 :
ホルモン補充療法中のエストロゲン濃度と骨代謝マーカーに関する検討,
Osteoporosis Japan, Vol.8, No.1, 50-52, 2000年. Takao Tamura, Minoru Irahara, Michiko Tezuka, Machiko Kiyokawa and Toshihiro Aono :
Orexins, Orexigenic Hypothalamic Neuropeptides, Suppress the Pulsatile Secretion of Luteinizing Hormone in Ovariectomized Female Rats,
Biochemical and Biophysical Research Communications, Vol.264, No.3, 759-762, 1999.

(要約): It is well known that feeding disorders are deeply related to reproductive dysfunction, and some feeding regulatory factors may modulate the reproductive function. We examined the effect of orexins, the newly discovered orexigenic hypothalamic neuropeptides, on the pulsatile secretion of LH to clarify their influence on the reproductive function. We administered orexins or saline into the third ventricle of bilaterally ovariectomized (OVX) rats, and measured the serum LH concentration by RIA in blood samples drawn every 6 min for 2 hours to analyze the pulsatile secretion. In the orexin-treated groups, the mean LH concentration and the pulse frequency were significantly reduced (p < 0.01), but the pulse amplitude did not differ significantly. These data indicate that orexins suppress the pulsatile secretion of LH by influencing GnRH neurons at the hypothalamic level.
(キーワード): Animals / Carrier Proteins / Female / Hypothalamus / Intracellular Signaling Peptides and Proteins / Luteinizing Hormone / Neuropeptides / Ovariectomy / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.1999.1573
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10544004; ● Search Scopus @ Elsevier (PMID): 10544004; ● Search Scopus @ Elsevier (DOI): 10.1006/bbrc.1999.1573

(DOI: 10.1006/bbrc.1999.1573, PubMed: 10544004) 安井敏之, 木下宏実, 山田正代, 苛原稔, 青野敏博, 上村浩一, 米田直人, 砂原誠司, 三浦雅一, 栗本文彦 :
骨減少症ならびに骨粗鬆症に対するエストロゲン·プロゲスチンの連日投与法と隔日投与法の比較, --- 血中エストラジオール濃度との関係 ---,
Osteoporosis Japan, Vol.7, No.2, 166(284)-167(285), 1999年. Seiichiro Miyamoto, Minoru Irahara, Kenjiro Ushigoe, Akira Kuwahara, Hiromu Sugino and Toshihiro Aono :
Effects of activin on hormone secretion by signal female rat pituitary cells: Analysis by cell immunoblot assay,
The Journal of Endocrinology, Vol.161, No.3, 375-382, 1999.

(要約): We investigated the effect of activin A on secretion of LH, FSH, and prolactin (PRL) by female cultured rat pituitary cells at the single-cell level by means of the cell immunoblot assay. Anterior pituitary cells from 8-week-old female rats were preincubated with or without activin A for 24 h, after which they were monodispersed and immediately used for cell immunoblot assay. The percentages of LH-, FSH- and PRL-immunoreactive cell blots detected were 5.5, 5.3 and 43.1%, respectively, of all pituitary cells applied to the transfer membrane. The percentage of LH-secreting cells and mean LH secretion per cell did not change after treatment with activin. In contrast, activin significantly increased the percentage of FSH-secreting cells and mean FSH secretion per cell to 136.0 and 114. 5% respectively. In addition, activin significantly decreased the percentage of PRL-secreting cells and mean PRL secretion per cell to 52.2 and 72.0% respectively. These results suggest that (1) activin A has effects on female rat pituitary cells that increase not only the amount of FSH secretion per cell but also the number of FSH-secreting cells, and (2) activin A decreases both the amount of PRL secretion per cell and the number of PRL-secreting cells.
(キーワード): Activins / Animals / Cell Count / Cells, Cultured / Female / Follicle Stimulating Hormone / Gonadotropins, Pituitary / Growth Substances / Immunoblotting / Inhibins / Luteinizing Hormone / Pituitary Gland / Prolactin / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1677/joe.0.1610375
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10333540; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033064367

(DOI: 10.1677/joe.0.1610375, PubMed: 10333540, Elsevier: Scopus) Toshiyuki Yasui, M Yamada, H Kinoshita, Hirokazu Uemura, N Yoneda, Minoru Irahara, Toshihiro Aono, S Unahara, Y Mito, F Kuritoto and K Hata :
Combination of automatic HPLC-RIA method for determination of estrone and estradiol in serum,
Journal of Clinical Laboratory Analysis, Vol.13, No.6, 266-272, 1999.

(要約): We developed a highly sensitive assay for estrone and 17 beta-estradiol in serum. Estrone and 17 beta-estradiol, obtained by solid-phase extraction using a Sep pak tC18 cartridge, were purified by high-performance liquid chromatography (HPLC). Quantitation of estrone and 17 beta-estradiol were carried out by radioimmunoassay. Not insignificantly, this automatic system of extraction and HPLC succeeded in analyzing 80 samples a week. Intra-assay coefficients of variation (CV) for estrone and 17 beta-estradiol ranged from 19.5 to 28.7%, and from 8.5 to 13.7%, respectively. The minimum detectable dose for estrone and 17 beta-estradiol were 1.04 pg/ml and 0.64 pg/ml, respectively. The serum levels of 17 beta-estradiol using our method strongly correlated with those by Gas chromatography mass spectrometry (GC-MS). The serum levels of estrone and 17 beta-estradiol in 154 peri- and postmenopausal women were estimated to be between 15 and 27 pg/ml and between 3.5 and 24.0 pg/ml, respectively, while the serum level of 17 beta-estradiol in postmenopausal women, in particular, was estimated to be from 3.5 to 6.3 pg/ml. For postmenopausal women who suffered from vasomotor symptoms, the mean levels of estrone and 17 beta-estradiol at 12 to 18 hours after treatment with daily 0.625 mg conjugated equine estrogen (CEE) and 2.5 mg medroxyprogesterone acetate (MPA) were 135.0 and 21.3 pg/ml at 12 months, respectively. On the other hand, levels of estrone and 17 beta-estradiol at 12 to 18 hours after treatment with CEE and MPA every other day, were 73.4 and 15.3 pg/ml, respectively. These highly sensitive assays for estrone and 17 beta-estradiol are useful in measuring low levels of estrogen in postmenopausal women, and monitoring estrogen levels in women receiving CEE as hormone replacement therapy.
(キーワード): Adult / Aged / Chromatography, High Pressure Liquid / Estradiol / Estrone / Female / Gas Chromatography-Mass Spectrometry / Hormone Replacement Therapy / Humans / Male / Middle Aged / Postmenopause / Radioimmunoassay
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10633293; ● Search Scopus @ Elsevier (PMID): 10633293

(PubMed: 10633293) Hirokazu Uemura, Toshiyuki Yasui, N Yoneda, Minoru Irahara and T Aono :
Measurement of N- and C-terminal-region fragments of parathyroid hormone-related peptide in milk from lactating women and investigation of the relationship of their concentrations to calcium in milk.,
The Journal of Endocrinology, Vol.153, No.3, 445-451, 1997.

(要約): Parathyroid hormone-related peptide (PTHrP) is found in very high concentrations in the milk of various mammals. However, little is known about its physiological role in this fluid. To obtain detailed profiles of PTHrP in milk, we measured the concentrations of PTHrP in human milk by two different region-specific assays, PTHrP(1-87) (N-PTHrP) and PTHrP(109-141) (C-PTHrP). We also examined the correlations between PTHrP and Ca concentrations in milk as well as the correlations between PTHrP and secreted milk volume. The levels of N-PTHrP and C-PTHrP were relatively low after delivery and gradually increased to 13.87 +/- 2.40 nmol/l (mean +/- S.E.M.) and 56.39 +/- 11.31 nmol/l respectively on the 10th day postpartum. N-PTHrP concentration remained steady until the 6th month postpartum when weaning starts, at which point it decreased slightly. C-PTHrP levels changed in a similar way to N-PTHrP levels but were 2- to 5-fold higher. Milk Ca concentration, and content, correlated with C-PTHrP concentration, and content (r = 0.422 and r = 0.769 respectively; P < 0.0001) but not with N-PTHrP. N-PTHrP concentration in the milk samples on the 4th day postpartum correlated with the volume of milk secreted during the 24 h before the samples were taken (r = 0.524, P < 0.01), but C-PTHrP concentration did not. These results suggest that PTHrP in human milk may play some role in the maintenance of lactation through the N-terminal region and in promoting Ca transfer into milk via the C-terminal region.
(キーワード): Calcium / Female / Humans / Lactation / Milk, Human / Parathyroid Hormone / Parathyroid Hormone-Related Protein / Peptide Fragments / Peptides / Postpartum Period / Pregnancy / Proteins / Teriparatide
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9203999; ● Search Scopus @ Elsevier (PMID): 9203999

(PubMed: 9203999) Toshiyuki Yasui, Hirokazu Uemura, Minoru Irahara and Toshihiro Aono :
Effects of transforming growth factor-β on the production of parathyroid hormone-related peptide in a human ovarian cancer cell line in vitro,
Journal of Obstetrics and Gynaecology Research, Vol.23, No.3, 231-238, 1997. Haruhiko Saito, Eiji Hosoi, Ryuichi Yamasaki, Kazuhito Kameyama, Minoru Irahara, Yoshio Ohno and Shiro Saito :
Immunoreactive growth hormone-releasing hormone(IR-GHRH) in the feto-placental circulation and differential effects of L-Dopa, L-Arginine and Somatostatin-14 on the plasma levels of IR-GHRH in normal adults,
Hormone and Metabolic Research, Vol.29, No.4, 184-189, 1997.

(要約): The relation of the physiological releases of growth hormone-releasing hormone (GHRH) and growth hormone (GH) into the circulation in various conditions was investigated using a sensitive and specific radioimmunoassay for plasma GHRH. The mean fasting plasma level of immunoreactive (IR)-GHRH in 72 normal adults was 10.3 +/- 0.5 (mean +/- SEM) pg/ml and there was no significant sex difference in the level. The concentrations of IR-GHRH in plasma from the umbilical artery and umbilical vein were 107.3 +/- 20.5 pg/ml and 33.6 +/- 3.8 pg/ml, respectively, and a marked arterio-venous gradient was observed in all 12 individuals examined. The plasma level of IR-GHRH in the maternal vein was significantly lower than that in the cord blood, but was similar to that in non-pregnant women. In normal adults, although there was no apparent fluctuation in the level of plasma IR-GHRH or of plasma GH during bed rest, a significant increase of plasma IR-GHRH was detected followed by, or synchronized with the surge of plasma GH after oral administration of L-dopa. In contrast, on L-arginine infusion, no proportional elevation of plasma IR-GHRH with increase in plasma GH was observed. During and after intravenous infusion of somatostatin, the circulating IR-GHRH level did not increase, but on stopping the infusion there was an immediate and marked rebound surge of GH. We conclude that 1) the elevated IR-GHRH in the cord blood plasma originates from the fetus and may have a primary role in enhancing secretion of GH which promotes growth in early human life, and 2) the participations of GHRH in the mechanisms of GH secretion seen after administrations of L-dopa, L-arginine and somatostatin are different.
(キーワード): Adult / Arginine / Female / Fetal Blood / Growth Hormone-Releasing Hormone / Human Growth Hormone / Humans / Infant, Newborn / Levodopa / Maternal-Fetal Exchange / Pregnancy / Radioimmunoassay / Reference Values / Somatostatin / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1055/s-2007-979018
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9178029; ● Search Scopus @ Elsevier (PMID): 9178029; ● Search Scopus @ Elsevier (DOI): 10.1055/s-2007-979018

(DOI: 10.1055/s-2007-979018, PubMed: 9178029) Seiichiro Miyamoto, Kenjiro Ushigoe, Noriko Tamura, Akira Kuwahara, Keiji Shitsukawa, Keijiro Azuma, Minoru Irahara, Toshihiro Aono and Hiromu Sugino :
The effects of activin on prolactin secretion from individual GH3 cells: Analysis by the cell immunoblot assay. The Interaction of activin, activin receptors and gonadal steroids in rat pituitary,
3rd Ares-Serono Symposium on Inhibin, Activin, Follistatin - Recent Advances and Future Views, 1996. N Yoneda, Minoru Irahara, S Saito, Hirokazu Uemura and T Aono :
Usefulness of recombinant human prolactin for treatment of poor puerperal lactation in a rat model.,
European Journal of Endocrinology, Vol.133, No.5, 613-617, 1995.

(要約): Recombinant human prolactin (r-hPRL) was produced by a line of murine C127 cells transfected with human PRL gene. To assess the biological efficacy of r-hPRL in vivo, we studied its influence on milk secretion using a rat model in which lactation was reduced by bromocriptine treatment. Puerperal rats were injected daily for 9 days after delivery with bromocriptine or bromocriptine plus r-hPRL, and lactational performance was assessed by weighing the pups. The concentrations of rat and human PRL in rat serum were measured by specific radioimmunoassays and the mammary glands were examined on postpartum day 10. Daily injection of bromocriptine (0.1 mg/rat) significantly reduced the endogenous level of rat PRL and impaired the weight gain of the pups. Administration of r-hPRL increased the serum level of human PRL. Daily injections of r-hPRL (50 micrograms/rat, twice a day) restored lactational performance and significantly increased the weight of the pups. The detrimental effect of bromocriptine on the mammary glands, assessed by both weight and histological appearance, was reversed by administration of r-hPRL. These results demonstrate that r-hPRL is biologically active in vivo and replacement therapy of r-hPRL is effective in improving the lactational performance in bromocriptine-treated rats, and also that r-hPRL may be useful for the treatment of women with poor lactation.
(キーワード): Analysis of Variance / Animals / Birth Weight / Bromocriptine / Disease Models, Animal / Female / Hormone Antagonists / Humans / Lactation / Lactation Disorders / Mammary Glands, Animal / Postpartum Period / Prolactin / Radioimmunoassay / Random Allocation / Rats / Rats, Wistar / Recombinant Proteins / Transfection
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7581993; ● Search Scopus @ Elsevier (PMID): 7581993

(PubMed: 7581993) 安井敏之, 上村浩一, N Yoneda, 苛原稔, T Aono, S Sunahara, K Hata :
[Assessment of serum estrogen levels by RIA with HPLC during hormone replacement therapy].,
日本産科婦人科学会雑誌, Vol.47, No.7, 655-661, 1995年.

(要約): We studied the serum estradiol and estrone levels in 146 peri and postmenopausal women, and in 38 women who had complained of various climacteric disturbance symptoms during daily hormone replacement therapy (HRT) with conjugated equine estrogen (CEE) 0.625 mg and medroxyprogesterone acetate (MPA) 2.5 mg. Serum estradiol and estrone were measured before treatment, and at 6 months, and after one year of the HRT therapy by HPLC-radioimmunoassay. In 146 peri and postmenopausal women, the serum level of estradiol was from 3 to 6pg/ml. The serum level of estradiol in 38 women after HRT significantly increased (p < 0.01) from 3.34 to 23.6 pg/ml at 6 months, and 21.5 pg/ml at 12 months. The serum level of estrone significantly increased (p < 0.01) from 26.6pg/ml to 156.7pg/ml at 6 months, and 137.2pg/ml at 12 month. These results are very useful for deciding on the doses of hormones and the expected serum estradiol level in HRT for Japanese women.
(キーワード): Adult / Chromatography, High Pressure Liquid / Estradiol / Estrogen Replacement Therapy / Estrogens, Conjugated (USP) / Estrone / Female / Follicle Stimulating Hormone / Humans / Luteinizing Hormone / Medroxyprogesterone Acetate / Menopause / Middle Aged / Postmenopause / Radioimmunoassay / Uterine Hemorrhage
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 7636337; ● Search Scopus @ Elsevier (PMID): 7636337

(PubMed: 7636337) 安井敏之, 上村浩一, 米田直人, 苛原稔, 青野敏博, 砂原誠司, 畠啓視 :
ホルモン補充療法における血中エストロゲン値の高感度測定法による検討,
日本産科婦人科学会雑誌, Vol.47, No.7, 655-661, 1995年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1540009770382456064

(CiNii: 1540009770382456064) Yuji Yokoyama, Takanori Nakamura, Rikiya Nakamura, Minoru Irahara, Toshihiro Aono and Hiromu Sugino :
Identificaiton of activins and follistatin proteins in human follicular fluid and placenta,
The Journal of Clinical Endocrinology and Metabolism, Vol.80, No.3, 915-921, 1995. 安井敏之, 苛原稔, 青野敏博, 高橋久寿, 猪野博保, 奈賀脩, 松下光彦, 阪口彰, 吉本忠弘, 岸恭也, 山下明美, 武本幹彦, 大野義雄, 高柳真, 長町典夫, 竹内悟, 三宅敏一 :
排卵障害患者に対するクロミフェン·当帰芍薬散併用療法の有用性の検討,
日本不妊学会雑誌, Vol.40, No.1, 82-91, 1995年. Toshiya Matsuzaki, Toshiyuki Yasui, Keijiro Azuma, Toshihiro Aono and Minoru Irahara :
Mechanism of anovulation in hyperprolactinemic amenorrhea determined by pulsatile gonadotropin-releasing hormone injection combined with human chorionic gonadotropin,
Fertility and sterility, Vol.62, No.6, 1143-1149, 1994.

(キーワード): Hyperprolactinemia / hyperprolactinemic anovulation / pulsatile GnRH treatment / estrogen-positive feedback / hCG

安井敏之, 上村浩一, 梶博之, 米田直人, 桑原章, 横山裕司, 斉藤誠一郎, 漆川敬治, 東敬次郎, 苛原稔, 青野敏博 :
骨粗鬆症に対するホルモン補充療法および活性型ビタミンDの有効性に関する検討,
Osteoporosis Japan, Vol.2, No.1, 37-39, 1994年. 安井敏之, 米田直人, 上村浩一, 梶博之, 桑原章, 横山祐司, 斉藤誠一郎, 漆川敬治, 東敬次郎, 苛原稔, 青野敏博 :
中高年婦人および両側卵巣摘出婦人に対するエストロゲン·プロゲスチン持続併用療法の骨および脂質に対する検討,
日本更年期医学会雑誌, Vol.2, No.1, 130-138, 1994年.

(キーワード): Climacterium / Hormone replacement therapy / Lipid metabolism / Genital bleeding

安井敏之, 苛原稔, 鎌田正晴, 青野敏博 :
婦人科癌における小柴胡湯の抗腫瘍効果及び作用機序に関する検討,
和漢医薬学雑誌, Vol.11, No.3, 161-167, 1994年.

(キーワード): Sho-saiko-to / gynecological cancer / TGF-β / MTT / assay

Toshikazu Miyake, Minoru Irahara, Keiji Shitukawa, Toshiyuki Yasui and Toshihiro Aono :
Interaction of Activin A and Gonadal Steroids on FSH Secretion from Primary Cultured Rat Anterior Pituitary Cells,
Biochemical and Biophysical Research Communications, Vol.194, No.1, 413-419, 1993. Toshiyuki Yasui, Minoru Irahara, K Shisukawa, K Azuma and Toshihiro Aono :
The mechanism of the effect of combination treatment with clomiphene and bromocriptine in patients with normoprolactinemic anovulation,
Journal of Endocrinological Investigation, Vol.13, No.7, 549-554, 1990.

(キーワード): Normoprolactinemia / clomiphene citrate / bromocriptine / combination treatment / LH pulsatility

篠原紀美代, 渡邊博文, 細井英司, 齋藤史郎, 苛原稔, 山崎淳, 青野敏博 :
血液型A1型で抗B抗体の欠損した1例について,
日本輸血学会雑誌, Vol.36, No.3, 458-461, 1990年. 安井敏之, 青野敏博, 苛原稔, 高橋久寿, 古田順一, 猪野博保, 坂本康紀, 井川昭, 高橋弘子, 奈賀脩, 阪口彰, 吉本忠弘, 岸恭也, 山下明美, 河野美香, 高柳真, 鎌田正晴, 前川正彦, 鎌田敏雄, 長町典夫, 中郷吉二郎, 井原正之, 黒岩俊一郎, 岡山哲也, 竹内悟, 三木鈴, 藤島一典 :
クロミフェン無効の正プロラクチン血性排卵障害症例に対するクロミフェンー温経湯併用療法の検討,
日本不妊学会雑誌, Vol.35, No.1, 86-93, 1990年. 安井敏之, 坂本康紀, 植田敏弘, 前田信彦, 漆川敬治, 古本博孝, 森一正, 大頭敏文, 苛原稔, 鎌田正晴, 青野敏博 :
婦人科悪性腫瘍組織に発現するThomsen-Friedenreich抗原とその血中抗体価に関する臨床的研究,
日本産科婦人科学会雑誌, Vol.40, No.5, 583-587, 1988年.

(キーワード): Thomsen-Friedenreich antigen / T antibody / Gynecological malignancy

Takahide Mori, Minoru Irahara, Haruhiko Saito, Yoshio Ohno and Eiji Hosoi :
Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova,
Acta Endocrinol., Vol.110, No.3, 408-412, 1985. Takahide Mori, Haruhiko Saito, Yoshio Ohno, Minoru Irahara, Eiji Hosoi and Shiro Saito :
Evidence for existence of somatostatin-like immunoreactivity with molecular heterogeneity in porcine ovaries,
Acta Endocrinol., Vol.106, No.2, 254-259, 1984.

MISC

苛原稔 :
女性医学を考える上でのエストラジオール製剤の役割,
日本更年期医学会ニューズレター, No.17, 6-7, 苛原稔 :
女性医学を考える,
日本更年期医学会ニューズレター, No.16, 10, 篠原文香, 峯田あゆか, 乾宏彰, 香川智洋, 阿部彰子, 西條康代, 西村正人, 苛原稔, 岩佐武 :
術前に卵巣原発胚細胞性腫瘍を疑ったが, 傍卵巣の平滑筋肉腫であった一例,
現代産婦人科, Vol.70, No.1, 227-231, 2021年.

(キーワード): 骨盤内腫瘍 / 平滑筋肉腫 / 画像評価 / 栄養血管 / pelvic tumor / leiomyosarcoma / image evaluation / feeding blood vessels / コツバンナイシュヨウ / ヘイカツキンニクシュ / ガゾウヒョウカ / エイヨウケッカン
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520290885604307712

(CiNii: 1520290885604307712) 田村公, 山本由理, 湊沙希, 柳原里江, 鎌田周平, 谷口友香, 阿部彰子, 西村正人, 苛原稔, 岩佐武 :
子宮内膜癌・子宮内膜異型増殖症に対する妊孕性温存治療の治療効果と妊娠成績，周産期予後,
現代産婦人科, Vol.70, No.1, 123-128, 2021年.

(キーワード): 子宮内膜癌 / 子宮内膜異型増殖症 / MPA療法 / 妊孕性温存 / endometrial cancer / atypical endometrial hyperplasia / medroxyprogesterone acetate (MPA) treatment / シキュウナイマクガン / シキュウナイマクイケイゾウショクショウ / MPA リョウホウ / ニンヨウセイオンゾン
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520853835559930496

(CiNii: 1520853835559930496) 河北貴子, 加藤剛志, 門田友里, 笠井可菜, 吉田加奈子, 苛原稔, 岩佐武 :
当院産科婦人科外来の乳がん検診の現状,
徳島産婦人科医報, No.54, 4-8, 2021年. 吉本夏実, 加地剛, 祖川英至, 吉田あつ子, 米谷直人, 苛原稔, 岩佐武 :
徳島県における胎児超音波スクリーニング向上のための取り組み,
超音波TECHNO, Vol.33, No.4, 1-6, 2021年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520010381294671360

(CiNii: 1520010381294671360) 青木秀憲, 米谷直人, 加地剛, 祖川英至, 吉田あつ子, 苛原稔, 岩佐武 :
妊娠中に初めて診断されるも自然軽快した下垂体腺腫の一例,
現代産婦人科, Vol.69, No.2, 257-260, 2021年.

(キーワード): 下垂体 / 早産 / 非機能性下垂体腺腫 / 妊娠 / プロラクチノーマ / non-functional pituitary adenoma / pituitary gland / pregnancy / preterm delivery / prolactinoma / カスイタイ / ソウザン / ヒキノウセイカスイタイセンシュ / ニンシン
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520853834956639872

(CiNii: 1520853834956639872) 武田明日香, 加地剛, 今泉絢貴, 祖川英至, 吉田あつ子, 米谷直人, 西村正人, 苛原稔, 岩佐武 :
帝王切開時に子宮内にバルーンを留置し二期的子宮摘出(planned delayed hysterectomy)を行った前置癒着胎盤の2例,
現代産婦人科, Vol.69, No.2, 233-238, 2021年.

(キーワード): 前置癒着胎盤 / 二期的手術 / 子宮内バルーン / placenta accrete spectrum / planned delayed hysterectomy / intrauterine tamponade balloon / ゼンチユチャクタイバン / ニキテキシュジュツ / シキュウナイバルーン
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520853834956961920

(CiNii: 1520853834956961920) 河北貴子, 加藤剛志, 門田友里, 笠井可菜, 吉田加奈子, 苛原稔 :
子宮腺筋症，子宮内膜症のホルモン剤内服中に発見された乳癌4症例の後方視的検討,
日本産婦人科乳腺医学会雑誌, No.4, 42-46, 2021年. 井上麻由, 上田美香, 加地剛, 黒濱綾子, 田山正伸, 中川竜二, 中瀧理仁, 橋本公子, 橋本直子, 春名充, 前川正彦, 松下由喜代, 宮上和美, 米谷直人, 苛原稔, 葉久真理 :
徳島県妊産婦メンタルケア対策早期発見と支援のポイント(解説),
徳島産婦人科医報, No.52, 130-149, 2019年. 上田美香, 沖津修, 加地剛, 谷洋江, 中川竜二, 舩戸豊子, 春名充, 福井理仁, 別宮史朗, 前川正彦, 増矢幸子, 苛原稔 :
徳島県周産期災害対策マニュアル(解説),
徳島産婦人科医報, No.52, 151-177, 2019年. 矢野清人, 松崎利也, 柳原里江, 岩佐武, 苛原稔 :
産婦人科外来パーフェクトガイド : いまのトレンドを逃さずチェック!, --- 思春期・月経異常視床下部・下垂体性無月経. ---,
臨床婦人科産科, Vol.72, No.4, 64-68, 2018年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522262180530465536

(CiNii: 1522262180530465536) 門田友里, 西村正人, 炬口恵理, 河北貴子, 阿部彰子, 苛原稔 :
カルチノイド成分を合併した粘液生卵巣癌の1例,
現代産婦人科, Vol.64, No.2, 321-325, 2016年. 安井敏之, 松井寿美佳, 谷杏奈, 加藤剛志, 苛原稔 :
HRTの慎重投与例への対応 -7.慢性肝疾患，胆嚢炎および胆石症の既往,
産科と婦人科, Vol.80, No.12, 1609-1616, 2013年.

(キーワード): ホルモン補充療法 / 有害事象
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522825130123686400

(CiNii: 1522825130123686400) 苛原稔 :
子宮内膜症のおける薬物療法,
OG SCOPE, Vol.3, No.2, 0, 2012年. 加地剛, 前田和寿, 須藤真功, 中山聡一朗, 佐藤美紀, 苛原稔 :
Dual Dopplerを用いた肝静脈及び下行大動脈血流同時記録による胎児不整脈評価の実際,
MEDIX, Vol.56, 2012年. 高井繁美, 楊河宏章, 宮本登志子, 明石晃代, 井上弘美, 久米亜紀子, 田島壮一郎, 佐藤千穂, 西条伴香, 井本淳一郎, 鈴木あかね, 山上真樹子, 浦川典子, 下村智子, 片島るみ, 苛原稔, 和泉唯信, 梶龍兒, 西田善彦, 橋本和典, 近藤彰, 櫻木章司, 宇都宮正登, 川島周, 徳島治験ネットワーク :
「徳島治験ネットワーク」における治験の共同実施について国際共同治験実施の現状,
四国医学雑誌, Vol.66, No.1-2, 48, 2010年.

(キーワード): 国際協力; 神経系疾患(治療); 精神障害(治療); *臨床試験; 治験コーディネーター / Site Management Organization / ヒト (Homo sapiens)

安井敏之, 苛原稔, 鎌田正晴, 青野敏博 :
婦人科癌における小柴胡湯の抗腫瘍効果に関する検討,
Journal of Traditional Medicines, Vol.11, 346-347, 1994年. 安井敏之, 米田直人, 桑原章, 横山裕司, 松崎利也, 斉藤誠一郎, 漆川敬治, 東敬次郎, 苛原稔, 青野敏博 :
ラット下垂体腫瘍細胞のプロラクチン分泌に及ぼす漢方薬の効果に関する検討,
産婦人科漢方研究のあゆみ, No.10, 27-31, 1993年. 安井敏之, 高木秀学, 植田敏弘, 漆川敬治, 三宅敏一, 東敬次郎, 苛原稔, 青野敏博 :
モノクロナール抗体を用いた逆受身赤血球凝集反応によるプロラクチン測定法の基礎的検討とその臨床的応用, --- 赤血球凝集反応による新しいプロラクチン測定試薬 ---,
ホルモンと臨床, Vol.36, No.6, 105(605)-101(601), 1988年. 安井敏之, 苛原稔, 山野修司, 中山孝善, 黒岩俊一郎, 岸恭也, 永田由利子, 後藤哲也, 重清俊雄, 齋藤史郎, 矢野一郎, 上田隆 :
治療に抵抗を示し，著明な血小板減少を来した再生不良性貧血合併妊娠の1症例,
産科と婦人科, Vol.55, No.1, 115-118, 1988年.

(キーワード): pregnancy / aplastic anemia

総説・解説

苛原稔 :
女性医学が乳癌検診受診率向上の一助になれば,
日本乳癌検診学会誌, Vol.19, No.1, 1, 岩佐武, 松崎利也, 苛原稔 :
メトホルミンの多嚢胞性卵巣症候群と不妊に対するエビデンスとメカニズム,
内分泌·糖尿病·代謝内科, Vol.49, No.3, 203-206, 2019年9月.

(キーワード): PCOS / metformine / clomiphene / insulin resistance
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521417755892423296

(CiNii: 1521417755892423296) 岩佐武, 松崎利也, 苛原稔 :
無排卵・機能性不妊に対する卵巣刺激低用量漸増療法,
臨床婦人科産科, Vol.73, No.7, 644-649, 2019年7月. 岩佐武, 苛原稔, 松崎利也 :
多嚢胞性卵巣症候群の病態と診断,
思春期学, Vol.37, No.2, 185-189, 2019年6月. Takeshi Iwasa, Toshiya Matsuzaki, Kiyohito Yano, Yiliyasi Mayila, Rie Yanagihara, Yuri Yamamoto, Akira Kuwahara and Minoru Irahara :
Effects of Low Energy Availability on Reproductive Functions and Their Underlying Neuroendocrine Mechanisms.,
Journal of Clinical Medicine, Vol.7, No.7, Jul. 2018.

(要約): It is known that metabolic disturbances suppress reproductive functions in females. The mechanisms underlying metabolic and nutritional effects on reproductive functions have been established based on a large body of clinical and experimental data. From the 1980s to 1990s, it was revealed that disrupted gonadotropin-releasing hormone (GnRH) secretion is the main cause of reproductive impairments in metabolic and nutritional disorders. From the late 1990s to early 2000s, it was demonstrated that, in addition to their primary functions, some appetite- or metabolism-regulating factors affect GnRH secretion. Furthermore, in the early 2000s, kisspeptin, which is a potent positive regulator of GnRH secretion, was newly discovered, and it has been revealed that kisspeptin integrates the effects of metabolic status on GnRH neurons. Recent studies have shown that kisspeptin mediates at least some of the effects of appetite- and metabolism-regulating factors on GnRH neurons. Thus, kisspeptin might be a useful clinical target for treatments aimed at restoring reproductive functions in individuals with metabolic or nutritional disturbances, such as those who exercise excessively, experience marked weight loss, or suffer from eating disorders. This paper presents a review of what is currently known about the effects of metabolic status on reproductive functions and their underlying mechanisms by summarizing the available evidence.
(徳島大学機関リポジトリ): ● Metadata: 117743
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/jcm7070166
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29976877; ● Search Scopus @ Elsevier (PMID): 29976877; ● Search Scopus @ Elsevier (DOI): 10.3390/jcm7070166

(徳島大学機関リポジトリ: 117743, DOI: 10.3390/jcm7070166, PubMed: 29976877) 苛原稔 :
1.近未来の生殖医療 (2)子宮移植:概要と課題VS倫理的問題点 B:子宮移植と倫理的問題,
研修ノートNo.100「産婦人科医療の近未来」, No.100, 2018年1月. 岩佐武, 松崎利也, 矢野清人, 苛原稔 :
産婦人科画像診断トレーニング:多嚢胞性卵巣症候群の画像診断とその臨床的意義は?,
臨床婦人科産科, Vol.71, No.4, 199-202, 2017年4月. 鎌田正晴, 濱田信一, 苛原稔 :
産婦人科における乳がん検診法のあり方, --- 特にマンモグラフィと超音波の総合判定について ---,
日本女性医学学会雑誌, Vol.24, No.2, 254-257, 2017年4月. 矢野清人, 松崎利也, 苛原稔 :
多嚢胞性卵巣症候群テストステロン,
最新女性医療, 2017年2月. 矢野清人, 松崎利也, 苛原稔 :
卵胞発育異常の病態と治療多嚢胞性卵巣症候群,
臨床婦人科産科, Vol.70, No.12, 1117-1123, 2016年12月. 宇都宮隆史, 見尾保幸, 田中温, 苛原稔 :
生殖補助医療における黄体補充を受ける女性を対象としたAKP-008(ルテウム®膣用坐剤400mg)の有効性と安全生の検討(第Ⅲ相臨床試験),
新薬と臨床, Vol.65, No.12, 2016年12月. 阿部彰子, 西村正人, 炬口恵理, 河北貴子, 苛原稔 :
Peutz-Jeghers症候群に合併した若年子宮体癌の1例,
日本婦人科腫瘍学会雑誌, Vol.34, No.4, 640-647, 2016年10月.

(キーワード): uterine body / Peutz-Jeghers syndrome / young endometrial cancer
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520291855499270528

(CiNii: 1520291855499270528) 岩佐武, 松崎利也, 苛原稔 :
中枢性月経異常,
臨床婦人科産科, Vol.70, No.6, 481-485, 2016年6月. 苛原稔 :
女性ヘルスケアのための生殖内分泌研究の意義,
日本女性医学学会雑誌, Vol.24, No.1, 64-70, 2016年1月.

(キーワード): 女性ヘルスケア / 生殖内分泌 / 多嚢胞性卵巣症候群 / 乳房管理 / 更年期
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572357874341760

(CiNii: 1520572357874341760) 岩佐武, 松崎利也, 苛原稔 :
卵巣機能とストレス反応,
Hormone Frontier in Gynecology, Vol.21, No.4, 21-25, 2014年12月. 苛原稔 :
脂質異常症とHRT「女性の脂質異常症の治療と管理ポイント」,
Progress in Medicine, Vol.34, No.9, 2014年9月. 松崎利也, 岩佐武, 苛原稔 :
アディポサイトカインとゴナドトロピン分泌,
HORMONE FRONTIER IN GYNRCOLOGY, Vol.21, No.3, 31-37, 2014年9月. 苛原稔, 桑原章 :
出生前診断と着床前診断の倫理問題,
公衆衛生, Vol.78, No.3, 163-166, 2014年3月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282763027646080

(CiNii: 1390282763027646080) 松崎利也, 岩佐武, 苛原稔 :
新たに開発されたアーキテクト・テストステロンⅡの性能評価と多囊胞性卵巣症候群(PCOS)の診断における有用性の検討,
医学と薬学, Vol.70, No.20, 331-339, 2013年8月. 苛原稔 :
産婦人科診療における乳癌検診の重要性,
日本産科婦人科学会雑誌, Vol.65, No.8, 1152-1153, 2013年. 苛原稔 :
ARTの副作用と合併症,
母子保健情報, No.66, 39-43, 2012年12月.

(キーワード): 生殖補助医療(ART) / 多胎妊娠 / 卵巣過剰刺激症候群 / 流産 / 異所性妊娠
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521417755404777728

(CiNii: 1521417755404777728) 苛原稔 :
不妊症治療の立場から, --- 不妊治療の歴史と未来 ---,
周産期医学, Vol.42, No.8, 959-962, 2012年8月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523951030379780992

(CiNii: 1523951030379780992) 木内理世, 松崎利也, 松井寿美佳, 中澤浩志, 苛原稔 :
エクルーシス試薬エストラジオールⅢの基礎的および臨床的有用性についての検討,
医学と薬学, Vol.68, No.2, 319-326, 2012年8月. 松崎利也, 木内理世, 中澤浩志, 苛原稔 :
OHSSを防ぐ排卵誘発,
産科と婦人科, Vol.79, No.7, 875-882, 2012年7月.

(キーワード): クロミフェン-メトホルミン併用療法 / GnRHパルス療法 / FSH低用量漸増療法
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520291853577555584

(CiNii: 1520291853577555584) 鎌田正晴, 炬口友里, 山田正代, 岡田真澄, 横山裕司, 漆川敬治, 苛原稔 :
乳腺疾患—乳がん検診を中心に,
産科と婦人科, Vol.79, No.4, 166-170, 2012年4月. 苛原稔 :
やせと肥満,
日本医師会雑誌, Vol.141, 113-115, 2012年. 松井寿美佳, 松崎利也, 山本哲史, 苛原稔 :
多嚢胞性卵巣症候群(PCOS)のおける心血管疾患リスクの評価と管理,
産科と婦人科, Vol.78, No.12, 1495-1500, 2011年11月.

(キーワード): PCOS / インスリン抵抗性 / 心血管疾患
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523388081036175872

(CiNii: 1523388081036175872) 松崎利也, 岩佐武, 木内理世, 苛原稔 :
新しい排卵誘発法,
産婦人科治療, Vol.102, No.102, 753-760, 2011年5月.

(キーワード): Ovulation induction / Metformin / Low-dose FSH therapy / self injection
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523106603289651840

(CiNii: 1523106603289651840) 苛原稔, 桑原章, 田中優, 谷口友香 :
ARTにおける各種卵巣刺激の適応とプロトコール,
産婦人科の実際, Vol.60, No.5, 677-684, 2011年5月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520010380630913536

(CiNii: 1520010380630913536) 松井寿美佳, 安井敏之, 加地剛, 山本哲史, 上村浩一, 苛原稔 :
周閉経期におけるNT-proBNPの意義,
日本女性医学学会雑誌, Vol.19, No.1, 37-41, 2011年4月.

(キーワード): NT-proBNP / SHBG / Perimenopause / Postmenopause
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520290882653580160

(CiNii: 1520290882653580160) 木内理世, 松崎利也, 苛原稔 :
視床下部・下垂体性排卵障害,
臨床婦人科産科, Vol.65, No.4, 465-473, 2011年4月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522262179969693952

(CiNii: 1522262179969693952) 前田和寿, 前川正彦, 苛原稔 :
風疹抗体,
臨床検査ガイド 2011∼2012, 762-764, 2011年3月. 前田和寿, 須藤真功, 佐藤美紀, 加地剛, 苛原稔 :
【どんな異常も見逃さない! 産科急変のシグナルとベスト対応】分娩期肩甲難産(解説/特集),
ペリネイタルケア, 81-87, 2011年1月. 松崎利也, 岩佐武, 木内理世, 苛原稔 :
多嚢胞性卵巣症候群,
産科と婦人科, Vol.77, No.11, 1325-1333, 2010年11月.

(キーワード): 排卵障害 / 男性ホルモン過剰 / 多嚢胞卵巣
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521980705730054656

(CiNii: 1521980705730054656) 苛原稔, 松崎利也, 桑原章 :
排卵誘発,
産婦人科の実際 (特集外来診療マニュアル), Vol.59, No.11, 1850-1855, 2010年10月. 岩佐武, 松崎利也, 苛原稔 :
ストレス・摂食調節因子によるゴナドトロピン分泌制御 (今月の臨床ここが問題若年女性のやせ・肥満),
臨床婦人科産科, Vol.64, No.9, 1293-1299, 2010年9月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520010380416960128

(CiNii: 1520010380416960128) 苛原稔, 松崎利也, 岩佐武, 桑原章 :
不妊治療と多嚢胞性卵巣症候群 (特集妊孕能の可能性と限界を考える),
産婦人科の実際, Vol.59, No.5, 705-710, 2010年5月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1524232505135871872

(CiNii: 1524232505135871872) 岩佐武, 松崎利也, 苛原稔 :
摂食とストレスによる生殖機能調節への影響 (特集生殖機能調節の新しい視点),
産科と婦人科, Vol.77, No.3, 265-272, 2010年3月.

(キーワード): GnRH / Kisspeptin
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521136279821149824

(CiNii: 1521136279821149824) Kamada Masaharu, Masahiko Maegawa, Maeda Nobuhiko, Satoshi Yamamoto, Yoshikawa Shouji, Mori Hidetoshi, Yuu Tanaka, Okada Masumi, Shitukawa Keiji, Minoru Irahara, Toshihiro Aono, Hiroshi Kido, Ogushi Fumitaka, Sono Saburo, Kanayama Naoomi, Susumu Kagawa, Ohmoto Yasukazu, Futaki Shiro, Liang Guo Zhi and Koide S. Samuel :
5. Immunoglobulin binding factor in human seminal plasma and uterine cerivical mucus,
Current Paradigm of Reproductive Immunology, 95-104, Apr. 2009. 苛原稔, 上村浩一, 桑原章, 松崎利也, 安井敏之 :
妊孕能温存の婦人科がん治療】婦人科がん治療後の妊孕性,
産婦人科の実際, Vol.58, No.3, 317-322, 2009年3月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521980704924909056

(CiNii: 1521980704924909056) 安井敏之, 山田正代, 上村浩一, 苛原稔, 土屋直子, 譲原光利, 加瀬義夫 :
【更年期の女性医学】ホットフラッシュとサイトカイン,
産婦人科の世界, Vol.59, No.9, 813-819, 2007年9月. 安井敏之, 上村浩一, 苛原稔 :
【中高年女性のトータルヘルスケア】更年期障害と関連疾患更年期障害その実態とケア,
臨床婦人科産科, Vol.61, No.7, 877-881, 2007年7月. 安井敏之, 加地剛, 山田正代, 上村浩一, 苛原稔 :
【産婦人科医に必要な骨粗鬆症の知識とくにSERMについて】わが教室における骨粗鬆症診療の実際,
産婦人科治療, Vol.94, No.5, 996-1001, 2007年5月.

(キーワード): 骨粗鬆症 / ホルモン補充療法 / 切迫流産
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522262180732313344

(CiNii: 1522262180732313344) 安井敏之, 冨田純子, 宮谷友香, 山田正代, 上村浩一, 苛原稔 :
【中高年女性のQOL向上を目指して】わが教室における更年期障害の治療,
産婦人科治療, Vol.93, No.1, 89-96, 2006年7月.

(キーワード): ホルモン補充療法 / 漢方治療 / 東西融合治療
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522543655038814720

(CiNii: 1522543655038814720) 安井敏之, 冨田純子, 山田正代, 上村浩一, 苛原稔 :
【SERMを使いこなす】SERMの安全性心血管系・脂質代謝への影響,
性差と医療, Vol.3, No.7, 735-740, 2006年6月. 安井敏之, 宮谷友香, 冨田純子, 山田正代, 上村浩一, 苛原稔 :
【女性ホルモンUp to Date】女性ホルモン測定の意義,
性差と医療, Vol.3, No.2, 153-159, 2006年1月. 上村浩一, 安井敏之, 苛原稔 :
【特集】女性の腰痛とその対策更年期にみられる腰痛とその対策,
産婦人科治療, Vol.92, No.2, 147-151, 2006年. 安井敏之, 苛原稔 :
月経異常，排卵障害への漢方治療,
産婦人科の実際, Vol.54, No.9, 1343-1351, 2005年9月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523669555002855552

(CiNii: 1523669555002855552) 苛原稔, 桑原章, 後藤田浩司, 森川富昭 :
国立大学におけるDPC対応の実際, --- 徳島大学病院の取り組み ---,
病院新時代, 2005年8月. 安井敏之, 海野友香, 山田正代, 上村浩一, 苛原稔 :
ホルモン補充療法の効用,
産婦人科治療, Vol.90, No.5, 860-867, 2005年5月.

(キーワード): ホルモン補充療法 / 効用 / テーラーメイド

桑原章, 檜尾健二, 山野修司, 苛原稔 :
ARTにおける子宮外妊娠の発生と胚盤胞移植,
Journal of Mammalian Ova Research, Vol.22, No.2, 59-63, 2005年4月.

(要約): 子宮外妊娠は補助生殖医療(AR)による妊娠の約5%に起こることから，その予防が重要な課題である．近年，胚盤胞移植により子宮外妊娠の頻度が減少する可能性が指摘されている．初期胚移植に比べて，胚盤胞移植では移植から着床までの期間が短いこと，また子宮の収縮が胚盤胞移植時期に少ないことから，移植胚が卵管へ輸送される可能性が低い胚盤胞移植は子宮外妊娠予防に有利とされる．一方，不妊原因である卵管因子の有無は子宮外妊娠の発生頻度に影響を与える．AR症例で卵管因子を有する症例の子宮外妊娠率は卵管因子の無い症例に比べて高い．胚盤胞移植と子宮外妊娠の関連を検討した報告では，卵管因子をもつ症例では初期胚移植に比べ胚盤胞移植で子宮外妊娠の発生が少ないが，卵管因子を持たない症例ではこの傾向は認められない．卵管因子を持つ症例にARを行う場合，移植時期を胚盤胞期にすることで子宮外妊娠の予防が期待される．
(キーワード): 体外受精-胚移植 / 胚盤胞移植 / 子宮外妊娠 / 卵管不妊
(出版サイトへのリンク): ● Publication site (DOI): 10.1274/jmor.22.59
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679315047808; ● Search Scopus @ Elsevier (DOI): 10.1274/jmor.22.59

(DOI: 10.1274/jmor.22.59, CiNii: 1390282679315047808) 安井敏之, 海野友香, 山田正代, 上村浩一, 苛原稔 :
産婦人科領域におけるホルモン補充療法の今後,
骨粗鬆症治療, Vol.4, No.1, 42-49, 2005年1月.

(キーワード): ホルモン補充療法 / テーラーメイドHRT

西岡安彦, 阿部真治, 楊河宏章, 苛原稔, 曽根三郎 :
樹状細胞による肺癌の免疫療法,
コンセンサス癌治療, Vol.4, No.4, 218-219, 2005年. 西岡安彦, 阿部真治, 楊河宏章, 苛原稔, 曽根三郎 :
特集:コンセンサス肺癌の治療，トピックス:樹状細胞による肺癌の免疫療法,
コンセンサス癌治療, Vol.4, No.4, 218-219, 2005年. 森本忠興, 駒木幹正, 上野淳二, 苛原稔 :
都道府県の乳がん部会と精中委とのあり方, --- 乳がん部会の現状 ---,
日本乳癌検診学会誌, Vol.13, No.2, 195-204, 2004年6月. 苛原稔, 上村浩一, 山田正代, 安井敏之 :
【更年期のヘルスケア】更年期に好発する症状·疾患とその対策,
産婦人科治療, Vol.88, No.6, 1296-1301, 2004年. 楊河宏章, 苛原稔 :
徳島大学における高度医療の支援基盤整備,
四国医学雑誌, Vol.59, No.6, 299-303, 2003年12月.

(キーワード): clinical trial / supporting system / 健康増進 (health promotion) / clinical research coordinator
(徳島大学機関リポジトリ): ● Metadata: 39808

(徳島大学機関リポジトリ: 39808) 苛原稔, 安井敏之, 上村浩一, 滝川稚也, 海野友香 :
【子宮内膜病変の評価と対策】機能性子宮出血の病態に基づく診断と対策,
産婦人科の世界, Vol.55, No.9, 1005-1010, 2003年9月. 檜尾健二, 堤ゆかり, 桑原章, 田中優, 苛原稔, 山野修司 :
GnRH Clinical Report 酢酸プセレリンを用いた採卵日固定法による胚盤胞移植の有用性の検討,
Hormone Frontier in Gynecology, Vol.10, No.2, 195-199, 2003年6月. 安井敏之, 苛原稔 :
不妊症と漢方,
産婦人科治療, Vol.86, No.5, 960-967, 2003年5月.

(キーワード): 不妊症 / 漢方薬 / エビデンス

安井敏之, 苛原稔 :
「HRT脱落例を抑えるためのわれわれの工夫」HRTによる副作用を抑えるための投与量の工夫,
日本更年期医学会雑誌, Vol.11, No.1, 125-132, 2003年4月. 曽根三郎, 楊河宏章, 苛原稔 :
大学病院での臨床試験支援体制の整備と課題,
がん分子標的治療, Vol.1, No.3, 220-227, 2003年. 安井敏之, 海野友香, 滝川稚也, 上村浩一, 苛原稔 :
【性ステロイド関連製剤その臨床的位置付け】エストロゲン関連製剤 (1)経口エストロゲン剤結合型エストロゲン,
HORMONE FRONTIER IN GYNECOLOGY, Vol.9, No.4, 327-339, 2002年12月.

(キーワード): 結合型エストロゲン / 更年期障害 / 骨脂質代謝 / 低用量ホルモン補充療法

苛原稔, 安井敏之, 上村浩一, 清川麻知子, 田中優 :
【不正性器出血その診断から治療まで】機能性出血の診断と治療,
産婦人科治療, Vol.84, No.5, 511-515, 2002年5月. 安井敏之, 苛原稔, 青野敏博 :
プロラクチン系,
ホルモンと臨床, Vol.50, 13-21, 2002年4月. 苛原稔, 山野修司, 青野敏博 :
ARTによる単胎妊娠を求めて,
産婦人科治療, Vol.83, No.1, 62-66, 2001年7月. 苛原稔, 安井敏之, 上村浩一 :
【ホルモン補充療法】HRTの副作用,
からだの科学, Vol.219, 69-73, 2001年5月. 苛原稔, 上村浩一, 鎌田正晴, 青野敏博 :
【婦人科医のための乳癌検診】女性のライフステージと乳房の変化,
臨床婦人科産科, Vol.55, No.4, 328-332, 2001年4月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523106603355326080

(CiNii: 1523106603355326080) 安井敏之, 苛原稔, 青野敏博 :
婦人科疾患と漢方,
産婦人科治療, Vol.82, No.3, 284-292, 2001年3月.

(キーワード): 漢方薬 / 婦人科疾患 / 併用療法 / Evidence based medicine

上村浩一, 苛原稔, 鎌田正晴, 青野敏博 :
【婦人科医のための乳癌検診】自己検診の指導法,
臨床婦人科産科, Vol.55, 374-377, 2001年. 鎌田正晴, 上村浩一, 前川正彦, 苛原稔, 青野敏博 :
【婦人科医のための乳癌検診】乳癌の発生とエストロゲン,
臨床婦人科産科, Vol.55, 344-351, 2001年. 安井敏之, 手束典子, 山田正代, 上村浩一, 苛原稔, 青野敏博 :
更年期女性にみられる精神神経症状,
四国医学雑誌, Vol.56, No.2, 35-39, 2000年4月.

(キーワード): psychological symptoms / postmenopausal women / hormone replacement therapy
(徳島大学機関リポジトリ): ● Metadata: 110438

(徳島大学機関リポジトリ: 110438) 安井敏之, 山田正代, 苛原稔, 青野敏博, 岡孝己 :
乳腺のプロラクチンレセプター,
産科と婦人科, Vol.67, No.2, 182-188, 2000年2月. 上村浩一, 安井敏之, 苛原稔, 青野敏博 :
乳腺におけるカルシウム能動運送と母乳量調節,
Clinical Calcium, Vol.9, No.12, 1526-1530, 1999年12月. 安井敏之, 手束典子, 山田正代, 上村浩一, 苛原稔, 青野敏博 :
冷え·のぼせを現代医学から,
漢方と最新治療, Vol.8, No.4, 295-300, 1999年11月.

(キーワード): sensation of cold / hot flush / hormone replacement therapy / Chinese medicine

安井敏之, 苛原稔, 青野敏博 :
更年期の出血, --- ホルモン異常 3 ---,
臨床婦人科産科, Vol.53, No.11, 1366-1369, 1999年11月. 安井敏之, 牛越賢治郎, 苛原稔, 青野敏博 :
排卵障害に対する当帰芍薬散の作用,
日経メディカル, No.383, 34-35, 1999年10月.

(キーワード): 当帰芍薬散 / 中枢への作用 / 卵巣への作用

上村浩一, 安井敏之, 苛原稔, 青野敏博 :
閉経後骨粗鬆症の治療,
四国医学雑誌, Vol.55, 202-206, 1999年.

(徳島大学機関リポジトリ): ● Metadata: 112159

(徳島大学機関リポジトリ: 112159) 安井敏之, 苛原稔, 青野敏博 :
プロラクチン受容体の発現機構,
HORMONE FRONTIER IN GYNECOLOGY, Vol.5, No.4, 35(343)-42(350), 1998年12月.

(キーワード): プロラクチン受容体 / long form / short form / JAK/STAT / ノックアウトマウス

講演・発表

Takashi Kaji, Eishi Sogawa, Atsuko Yoshida, 米谷直人, 前田和寿 and Minoru Irahara :
Visualization of the sagittal view of the anal canal and sphincters using the high-frequency linear transducer in normal fetuses: a feasibility study.,
30th World Congress on Ultrasound in Obstetrics and Gynecology, WEB, Oct. 2020. Takashi Kaji, Kazuhisa Maeda, Atsuko Hichijyo, Naoto Yonetani and Minoru Irahara :
Fetal hypospadias: association of its severity and prenatal diagnosis,
29th World Congress on Ultrasound in Obstetrics and Gynecology Berlin, Berlin, Oct. 2019.

(出版サイトへのリンク): ● Publication site (DOI): 10.1002/uog.21049
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1002/uog.21049

(DOI: 10.1002/uog.21049) Chisato Tonoiso, Hitoshi Ikushima, Akihiro Haga, Shunsuke Furutani, Takashi Kawanaka, Akiko Kubo, Masato Nishimura, Akiko Abe, Minoru Irahara and Masafumi Harada :
Investigation of prognostic factors of cervical cancer using pre-treatment MRI images,
The 2018 International Gynecologic Cancer Society Meeting, Sep. 2018. Sachi Kishida, Yumi Kuwamura, Toshiyuki Yasui, M Tuji, Issei Imoto and Minoru Irahara :
Characteristics of Japanese Pregnant Women Who Had Hereditary Counseling About Noninvasive Prenatal Genetic Testing (NIPT) In Tokushima,
2nd Technological Competency as Caring in the Health Sciences 2018, 80, Aug. 2018.

(キーワード): NIPT / Pregnant women / Hereditary counseling

Minoru Irahara :
New concept of diagnosis of PCOS,
The 103rd Annual Congress of KSOG, Seoul, Sep. 2017. Minoru Irahara :
Sympodium : PCOS and androgen excess,
The 22nd Seoul International Symposium, Seoul, Sep. 2017. Minoru Irahara :
Symposium:Androgen Excess in Women-Polycystic Ovary Syndrome,
The 8th Congress of the International Society for Gender Medicine, Sendai, Sep. 2017. Takashi Kaji, Yasunobu Hayabuchi, Kazuhisa Maeda, Atsuko Hichijyo, Naoto Yonetani, Takahashi Yohei, Nakayama Soichiro and Minoru Irahara :
Prenatal assessment of coronary artery anatomy using color Doppler in cases of D-transposition of the great arteries: case reports.,
Prenatal assessment of coronary artery anatomy using color Doppler in cases of D-transposition of the great arteries: case reports., Rome, Sep. 2016. Akira Kuwahara, Saito Hidekazu, Ishihara Osamu, Kugu Koji and Minoru Irahara :
Assisted Reproductive Technology (ART) in Japan, Annual reports of Japan ART registry.,
IFFS2016, India, Sep. 2016. YAMASAKI Mikio, Akira Kuwahara, TANIGUCHI Yuka, Yuri Yamamoto, Takeshi Iwasa, HINOKIO Kenji, Toshiya Matsuzaki and Minoru Irahara :
Comparison of AMH and FSH an indicator of ovarian reserve in ART.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Yuri Yamamoto, Akira Kuwahara, YANO Yuya, TANIGUCHI Yuka, YAMASAKI MIkio, HINOKIO Kenji and Minoru Irahara :
Clinical significance of serum hCG in early pregnancy periods in ART.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Akira Kuwahara, HINOKIO Kenji, TANIGUCHI Yuka, Yuri Yamamoto, YAMASAKI Mikio, Takeshi Iwasa and Minoru Irahara :
Clinical significance of estradiol and transvaginal ultrasonography for pregnancy outcomes in natural frozen-thawed blastcyst transfer cycles.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. TAKAHASHI Yohei, Takashi Kaji, NAKAYAMA Soichiro, HICHIJO Atsuko, Kazuhisa Maeda and Minoru Irahara :
Intrauterine death by cord entanglement in monoamniotic twin reversed arterial perfusion sequence after infertility treatment: a case report.,
IFFS/JSRM International Meeting 2015, Yokohama, Apr. 2015. HICHIJO Atsuko, TAKAHASHI Yohei, NAKAYAMA Soichiro, Takashi Kaji, NAKAOKU Daichi, MURAKAMI Masahiro, KONDO Akane, MORINE Mikio, Kazuhisa Maeda, Issei Imoto and Minoru Irahara :
Case of placental mesenchymal dysplasia associated with cytogenetic discrepancy between amniotic fluid cells and fetal blood.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. NAKAYAMA Soichiro, Takashi Kaji, Toshiyuki Yasui, HICHIJO Atsuko, TAKAHASHI Yohei and Minoru Irahara :
Serum 25(OH)D3 concentrations and season-specific correlates in Japanese pregnancy women.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Takashi Kaji, Toshiyuki Yasui, HICHIJO Atsuko, TAKAHASHI Yohei, NAKAYAMA Soichiro, Hirokazu Uemura, Kazuhisa Maeda and Minoru Irahara :
Effect of bed rest during pregnancy on bone turnover markers in pregnant and postpartum women.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Takeshi Iwasa, Toshiya Matsuzaki, KAWAMI Takako, MUNKHZAYA Mukhsaikhan, TUNGALAGSUVD Altankhuu and Minoru Irahara :
Hypothalamic Kiss1 and RFRP gene expressions are changed by a high dose of lipopolysaccharide in female rats.,
IFFS/JSRM International Meeting 2015, Yokohama, Apr. 2015. TUNGALAGSUVD Altankhuu, Toshiya Matsuzaki, Takeshi Iwasa, MUNKHZAYA Munkhsaikhan, KAWAMI Takako and Minoru Irahara :
Senktide does not recover suppression of HPG axis induced by acute fasting in male rats.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. MUNKHZAYA Munkhsaikhan, Toshiya Matsuzaki, Takeshi Iwasa, TUNGALAGSUVD Altankhuu, KAWAMI Takako and Minoru Irahara :
Suppressive effect of immune stress on LH secretion is absent in early neonatal period in rats.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Sumika Matsui, Toshiyuki Yasui, Kaoru Keyama, Takeshi Katou, Hirokazu Uemura and Minoru Irahara :
Ultra-low-dose estradiol improves arterial stiffness in postmenopausal women.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Takeshi Katou, KASAI Kana, Kaoru Keyama, Sumika Matsui, TANI Anna and Minoru Irahara :
Accidental findings of minimal endometriosis during laparoscopic surgery : a retrospective study.,
IFFS/JSRM International Meeting 2015, Yokohama, Apr. 2015. MAEGAWA Masahiko, TANI Anna, YAMAMOTO Satoshi, KUNIMI Kotaro, Takeshi Katou, Sumika Matsui, Kaoru Keyama, Hirokazu Uemura, Toshiyuki Yasui, KAMADA Masaharu, MITANI Ryuji, MIYATANI Yuka and Minoru Irahara :
Arterial stiffness is increased in young women with endometriosis.,
IFFS/JSRM International Meeting 2015, Yokohama, Apr. 2015. Kaoru Keyama, TANI Anna, Sumika Matsui, Takeshi Katou and Minoru Irahara :
LPS progress the development of endometriosis in murine model.,
IFFS/JSRM International Meeting 2015, Yokohama, Apr. 2015. Akiko Abe, Masato Nishimura, Eri Takiguchi, Takako Kawakita, TANIGUCHI Yuka, Yuri Yamamoto, YAMASAKI Mikio, Takeshi Iwasa, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Survey on oncologist and gynecologist concerned with fertility for cancer survivors in reproductive age.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Masato Nishimura, Eri Takiguchi, Takako Kawakita, Akiko Abe, TANIGUCHI Yuka, Yuri Yamamoto, YAMASAKI Mikio, Takeshi Iwasa, Akira Kuwahara, Toshiya Matsuzaki and Minoru Irahara :
Efficacy and pregnant outcomes of fertility-sparing treatment with medroxyprogesterone acetate for endometrial carcinoma and atypical endometrial hyperplasia in young women.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. SATO Kenji, SUEOKA Kou, SENBA Hitoshi, IINO Kotaro, SUZUKI Mariko, MIZUGUCHI Yuki, IZUMI Yoko, Minoru Irahara, YOSHIMURA Yasunori and TANAKA Mamoru :
Current status of preimplantation genetic dagonosis in Japan.,
IFFS/JSRM International Meeting,2015, Yokohama, Apr. 2015. Akira Kuwahara, Mikio Yamasaki, Fukushima Chiemi and Minoru Irahara :
Effect of elective cryopreservation of all embryo, Recent analysis of Japan ART registry.,
FFS Regional Meeting, Recent Progress and New Trends of ART in Japan, Boston, Oct. 2013. Nakazawa Hiroshi, Toshiya Matsuzaki, Takeshi Iwasa, Kinouchi Riyo, Gereltsetseg Ganbat and Minoru Irahara :
Early Postnatal Lipopolysaccharide Exposure : Effects on Adult Male Rat LH Response to Homotypic Stress.,
The Endocrine Society's 94th Annual Meeting & Expo, Houston, Jun. 2012. Gereltsetseg Ganbat, Toshiya Matsuzaki, Takeshi Iwasa, Kinouchi Riyo, Nakazawa Hiroshi and Minoru Irahara :
Delay in the Onset of Puberty of Intrauterine Growth Retarded Female Rats Cannot Be Rescued with Hypernutrition after Birth.,
The Endocrine Society's 94th Annual Meeting & Expo, Houston, Jun. 2012. Toshiya Matsuzaki, Gereltsetseg Ganbat, Takeshi Iwasa, Kinouchi Riyo, Nakazawa Hiroshi and Minoru Irahara :
Delay in the onset of puberty of intrauterine growth retarded female rats cannot be rescued by hypernutrition after birth.,
15th International & 14th European Congress of Endocrinology(ICE/ECE 2012), Florence, May 2012. Kazuhisa Maeda, Suto Masanori, Sato Miki, Takashi Kaji and Minoru Irahara :
What is the predictive factor of onset of labor pain after long term tocolysis treated with intravenous ritodorine for preterm labor?,
ISUOG, Los Angels, Sep. 2011. Takashi Kaji, Kazuhisa Maeda, Masanori Sutoh, Miki Sato and Minoru Irahara :
Assessment of fetal atrioventricular time intervals by dual Doppler in hepatic vein and descending aorta.,
21th World Congress on Ultrasound in Obstetrics and Gynecology, Los Angeles , USA, Aug. 2011. Toshiya Matsuzaki, Iwasa Takeshi, Kinouchi Riyo, Gereltsetseg Ganbat and Minoru Irahara :
Sensitivities of messenger ribonucleic acid expression levels of Kiss1 and its receptor, Kiss1r, to nutritional status are changed during the developmental period in female rats,
The Endocrine Society's 93rd Annual Meeting, Boston, Jun. 2011. Takashi Kaji, Kenji Mori, Souichirou Nakayama, Ryuuji Mitani, Kazuhisa Maeda, Shoji Kagami and Minoru Irahara :
Prenatal diagnosis of total anomalous pulmonary venous connection to the portal vein with absence of ductus venosus : A case report,
20th World Congress on Ultrasound in Obstetrics and Gynecology, Prague, Czech, Oct. 2010. Kazuhisa Maeda, Masanori Sutoh, Miki Satou, Takashi Kaji and Minoru Irahara :
Ultrasonographic and magnetic resonance imaging (MRI) detection of placenta accrete.,
20th World Congress on Ultrasound in Obstetrics and Gynecology, Prague, Czech, Oct. 2010. Takeshi Iwasa, Toshiya Matsuzaki, Shinobu Fujisawa, Riyo Kinouchi, Gereltsetseg Ganbat and Minoru Irahara :
Effects of Intra-Uterine Undernutrition on Hypothalamic Kiss1 Expression and the Timing of Puberty in Female Rats.,
The Endocrine Society's 92nd Annual Meeting, San Diego,CA,USA., Jun. 2010. K Tsutsui, VS Chowdhury, T Osugi, T Ubuka, Toshiya Matsuzaki, Takeshi Iwasa, Minoru Irahara, LJ Kriegsfeld and G Bentley :
Discovery of GnIH: first in birds and then in mammals Symposium: Gonadotropin inhibitory hormone (GnIH) functions in mammals.,
The Endocrine Society's 92nd Annual Meeting, San Diego,CA,USA., Jun. 2010. Toshiya Matsuzaki, Takeshi Iwasa, Shinobu Fujisawa, Riyo Kinouchi and Minoru Irahara :
Neonatal LPS Injection Alters the Body Weight Regulation Systems of Rats under Non-Stress and Immune Stress Conditions.,
The Endocrine Society's 92nd Annual Meeting, San Diego, CA ,USA., Jun. 2010. Toshiyuki Yasui, Hirokazu Uemura, Y Miyatani, M Yamada, N Tsuchiya, M Yuzurihara, S Takeda and Minoru Irahara :
Interleukin-8 and hot flushes in pre-, peri- and postmenopausal women and bilateral oophorectomized women.,
The 3rd Scientific Meeting of the Asian Pacific Menopause Federation (APMF), Taipei, Mar. 2007. Hirokazu Uemura, Toshiyuki Yasui, Y Miyatani, M Yamada, Mineyoshi Hiyoshi, Hideo Takeda, Kokichi Arisawa and Minoru Irahara :
Relationships between circulating osteoprotegerin and the risk factors of vascular disorders in postmenopausal women.,
The 3rd Scientific Meeting of the Asian Pacific Menopause Federation (APMF), Taipei, Mar. 2007. Kumiko Sakamoto, Yuki Hibino, Shuji Yamano, Kazuhisa Maeda, Minoru Irahara and Aiko Yamauchi :
Prescription drug use during pregnancy in Japan,
34th Annual Conference of the European Teratology Society, Abano Terme, Sep. 2006. Masahiko Maegawa, A Nakajima, Satoshi Yamamoto, Y Kasai, A Tani, D Kinoshita and Minoru Irahara :
Effects of ovarian hormones on adhesion and early development of autotransplanted endometrium in an immunocompetent murine model,
IX World Congress on Endometriosis, Maastricht(The Netherlands), Sep. 2005. A Nakajima, Masahiko Maegawa, Satoshi Yamamoto, A Tani, D Kinoshita, Y Kasai and Minoru Irahara :
The process of blood coagulation enhances early endometrial-peritrial interaction in a murine model of endometriosis,
IX World Congress on Endometriosis, Maastricht(The Netherlands), Sep. 2005. Masahiko Maegawa, Anna Tani, Ayako Nakajima, Dan Kinoshita, Yuka Sakai, Satoshi Yamamoto, Kazuhisa Maeda, Masahiko Maegawa, Masaharu Kamada and Minoru Irahara :
Changes on Pro-Inflammatory Cytokine Responses in Peripheral Blood During Normal Human Pregnancy,
IX International Congress of Reproductive Immunology, Vol.52, No.Supplement 1, 26, Kanagawa, Oct. 2004. Satoshi Yamamoto, Anna Tani, Ayako Nakajima, Dan Kinoshita, Yuka Kasai, Masahiko Maegawa, Masaharu Kamada, Yuji Okumura, Hiroshi Kido and Minoru Irahara :
Development of an Expression Vector Encoding Sperm Protein,Designated RSMP-B,as an Immunocontrraceptive Vaccine,
IX International Congress of Reproductive Immunology, Vol.52, No.Supplement 1, 55, Kanagawa, Oct. 2004. Kotaro Kunimi, Ayako Nakajima, Dan Kinoshita, Satoshi Yamamoto, Masahiko Maegawa, Masaharu Kamada and Minoru Irahara :
The Expression of Myeloid-Related Protein-8(MRP8: S100A8) and MRP14(S100A9) in the Human Uterine Cervix,
IX International Congress of Reproductive Immunology, Vol.52, No.Supplement 1, 58, Kanagawa, Oct. 2004. Ayako Nakajima, Anna Tani, Dan Kinoshita, Yuka Kasai, Satoshi Yamamoto, Masahiko Maegawa and Minoru Irahara :
A Repertoire of Cytokines in Human Cervical Mucus of Infertile Women,
IX International Congress of Reproductive Immunology, Vol.52, No.Supplement 1, 58, Kanagawa, Oct. 2004. Yuka Kasai, Anna Tani, Ayako Nakajima, Dan Kinoshita, hirokazu Uemura, Masahiko Maegawa, Toshiyuki Yasui, Masaharu Kamada and Minoru Irahara :
Pro-Inflammatory Cytokine Changes in Postmenopausal Women Treated with Hormone Replacement Therapy,
IX International Congress of Reproductive Immunology, Vol.52, No.Supplement 1, 57, Kanagawa, Oct. 2004. Junko Komatsu, Akira Kuwahara, Shuji Yamano and Minoru Irahara :
Lysophosphatidic acid promotes oocyte maturation in mice via MAP kinase of cumulus oocyte complex,
The 4th Conference of the Pacific Rim Society for Fertility and Sterility, Okinawa, Mar. 2004. Akira Kuwahara, Yu Tanaka, Yukari Tsutsumi, Kenji Hinokio, Toshiya Matsuzaki, Masahiko Maegawa, Toshiyuki Yasui, Shuji Yamano and Minoru Irahara :
Clinical outcomes of "true" unexplainedinfertility after laparoscopy,
The 4th Conference of the Pacific Rim Society for Fertility and Sterility, Okinawa, Mar. 2004. Naoko Moride, Yu Tanaka, Yukari Tsutsumi, Kenji Hinokio, Akira Kuwahara, Shuji Yamano and Minoru Irahara :
Cdc25C is related pronuclear formation fertilization of mouuse eggs,
The 4th Conference of the Pacific Rim Society for Fertility and Sterility, okinawa, Mar. 2004. Dan Kinoshita, Ayako Nakajima, Toshiko Kinoshita, Satoshi Yamamoto, Kazuhisa Maeda, Masahiko Maegawa, Minoru Irahara, Toshifumi Daitoh and Masaharu Kamada :
Changes in type 1 and type 2 cytokine production profile stimulated with mitogen during normal pregnancy,
The 4th Conference of the Pacific Rim Society for Fertility and Sterility, Okinawa, Mar. 2004. Masahiko Maegawa, Satoshi Yamamoto, Ayako Nakajima, Dan Kinoshita, Masayo Kanayama, Minoru Irahara, Masaharu Kamada, Yuji Okumura and Hiroshi Kido :
Development of an Expression Vector Encoding Sperm Protein,Designated rSMP-B,as an Immunocontrraceptive,
The 4th Conference of the Pacific Rim Society for Fertility and Sterility, Okinawa, Mar. 2004. R Ogata, Toshiyuki Yasui, Hirokazu Uemura, Machiko Kiyokawa, Masakazu Miura, Toshihiro Aono and Minoru Irahara :
Serum estradiol level that is required to achieve a sufficient increase in bone mineral density during hormone replacement therapy,
9th International Menopause Society World Congress on the Menopause, Berlin, Jun. 2002. Minoru Irahara, Masaharu Kamada, Hirokazu Uemura, Toshiyuki Yasui, Machiko Kiyokawa, Akira Kuwahara, Hiroshi Ikawa, Toshiya Matsuzaki and Toshihiro Aono :
Postmenopausal changes in production of type 1 and type 2 cytokines and the effects of hormone replacement therapy,
9th International Menopause Society World Congress on the Menopause, Berlin, Jun. 2002. Hirokazu Uemura, Toshiyuki Yasui, Machiko Kiyokawa, Akira Kuwahara, Hiroshi Ikawa, Toshiya Matsuzaki, Toshihiro Aono and Minoru Irahara :
Changes in the levels of circulating osteoprotegerin in case of estrogen deficiency,
9th International Menopause Society World Congress on the Menopause, Berlin, Jun. 2002. Minoru Irahara, Seiichiro Miyamoto, Kenjiro Ushigoe, Akira Kuwahara and Hiromu Sugino :
The effect of activin on hormone secretion from female rat pituitary cells: Analysis by cell immunoblot assay,
16th World Congress on Fertility and Sterility, San Francisco, Oct. 1998. Kenjiro Ushigoe, Akira Kuwahara, Seiichiro Miyamoto, Minoru Irahara, Hiromu Sugino and Toshihiro Aono :
Expression of activin receptors in rat pituitary cells,
16th World Congress on Fertiligy and Sterility, San Francisco, Oct. 1998. Minoru Irahara, Noriko Tamura, Akira Kuwahara, Kenjiro Ushigoe and Hiromu Sugino :
Effect of activin on production and secretion of prolactin in cultured rat GH3 cells,
15th World Congress of Gynecology and Obstetrics, Copenhagen, Aug. 1997. Seiichiro Miyamoto, Minoru Irahara, Kenjiro Ushigoe, Akira Kuwahara, Hiromu Sugino and Toshihiro Aono :
The effect of activin on hormone secretion from female rat pituitary cells: Analysis by cell immunoblot assay,
15th World Congress of Gynecology and Obstetrics, Copenhagen, Aug. 1997. Noriko Tamura, Kenjiro Ushigoe, Seiichiro Miyamoto, Akira Kuwahara, Keiji Shitsukawa, Keijiro Azuma, Minoru Irahara, Toshihiro Aono and Hiromu Sugino :
Effects of activin on production and secretion of prolactin in GH3 cells. The interaction of activin, activin receptors and gonadal steroids in rat pituitary,
3rd Ares-Serono Symposium on Inhibin, Activin, Follistatin - Recent Advances and Future Views, Tokushima, Nov. 1996. Akira Kuwahara, Noriko Tamura, Kenjiro Ushigoe, Seiichiro Miyamoto, Keiji Shitsukawa, Keijiro Azuma, Minoru Irahara, Toshihiro Aono and Hiromu Sugino :
Expression of activin receptors in rat pituitary cells. The Interaction of activin, activin receptors and gonadal steroids in rat pituitary,
3rd Ares-Serono Symposium on Inhibin, Activin, Follistatin - Recent Advances and Future Views, Tokushima, Nov. 1996. Toshihiro Aono, Akira Kuwahara, Noriko Tamura, Kenjiro Ushigoe, Seiichiro Miyamoto, Keiji Shitsukawa, M. Miyake, Keijiro Azuma, Minoru Irahara and Hiromu Sugino :
The interaction of activin, activin receptors and gonadal steroids in rat pituitary,
3rd Ares-Serono Symposium on Inhibin, Activin, Follistatin - Recent Advances and Future Views, Tokushima, Nov. 1996. Hiromu Sugino, Yuji Yokoyama, Takanori Nakamura, Kishiko Sugino, Rikiya Nakamura, Minoru Irahara and Toshihiro Aono :
Identification of activins and follistatin in human follicular fluid and placenta,
76th Annual Meeting of the American Endocrine Society, Anaheim, Jun. 1994. Yuji Yokoyama, Takanori Nakamura, Kishiko Sugino, Minoru Irahara, Hiromu Sugino and Toshihiro Aono :
Isolation of three isoforms of activins (A, AB and B) and follistatin in human follicular fluid,
8th Worlid Congress on in Vitro Fertilization and Alternate Assisted Reproduction, Kyoto, Sep. 1993. 藤川理恵, 高開登茂子, 久米博子, 三木幸代, 原田路可, 加根千賀子, 赤池雅史, 楊河宏章, 苛原稔 :
モンゴル国 A 病院のモンゴル人看護師のリスク感性の分析,
第53回日本看護学会学術集会, 2022年11月. 吉田加奈子, 門田友里, 笠井可菜, 峯田あゆか, 河北貴子, 加藤剛志, 西村正人, 苛原稔, 岩佐武 :
腹腔鏡下仙骨腟固定術(LSC)における後腹膜閉鎖の工夫,
第60回日本産婦人科内視鏡学会学術講演会, 2020年12月. 加地剛, 祖川英至, 吉田あつ子, 米谷直人, 早渕康信, 苛原稔 :
Dual Gate Dopplerを用いた肝静脈-下行大動脈血流波形による胎児不整脈評価の有用性と限界:当院での10年間の経験から,
第29回学術集会日本小児心筋疾患学会学術集会, 2020年11月. 加地剛, 吉本夏実, 祖川英至, 吉田あつ子, 米谷直人, 苛原稔 :
徳島県における胎児超音波スクリーニング向上のための取り組み:施設の集約化の面から,
日本超音波医学会第30回四国地方会学術集会, 2020年10月. 吉本夏実, 加地剛, 祖川英至, 吉田あつ子, 米谷直人, 苛原稔 :
徳島県における胎児超音波スクリーニング向上のための取り組み,
第93回日本超音波医学会, 2020年5月. 加地剛, 吉本夏実, 祖川英至, 吉田あつ子, 米谷直人, 苛原稔 :
リニアプローブを用いた胎児肛門矢状断像の描出率についての検討,
第93回日本超音波医学会, 2020年5月. 前田崇彰, 加地剛, 早渕康信, 中川竜二, 祖川英至, 吉田あつ子, 米谷直人, 須賀健一, 稲村昇, 苛原稔 :
胎児診断により動脈管閉鎖に伴う血行動態変化を観察できた左腕頭動脈孤立症の1例,
第93回日本超音波医学会, 2020年5月. 山本由理, 桑原章, 阿部彰子, 岩佐武, 倉橋浩樹, 苛原稔 :
徳島大学病院における初めての着床前診断(PGT-M)の経験,
第72回日本産科婦人科学会学術集会, 2020年4月. 吉田加奈子, 加藤剛志, 門田友里, 笠井可菜, 河北貴子, 桑原章, 苛原稔 :
当科における腹腔鏡下仙骨腟固定術(LSC)導入の現状,
第72回日本産科婦人科学会学術講演会, 2020年4月. Takashi Kaji, Eishi Sogawa, Atsuko Yoshida, Naoto Yonetani and Minoru Irahara :
Visualization of the sagittal view of the anal canal and sphincters using the high-frequency linear transducer in normal fetuses: a feasibility study.,
第72回日本産科婦人科学会学術集会, Apr. 2020. 圓藤幸子, 河北貴子, 祖川英至, 門田友里, 笠井可菜, 岩佐武, 吉田加奈子, 加藤剛志, 加地剛, 苛原稔 :
コンセプションケアからみた不妊治療後の子宮腺筋症合併妊娠の妊娠予後の検討,
第72回日本産科婦人科学会学術集会, 2020年4月. 武田明日香, 加地剛, 祖川英至, 吉田あつ子, 米谷直人, 苛原稔 :
胎児診断により動脈管閉鎖に伴う血行動態変化を観察できた左腕頭動脈孤立症(左腕頭動脈肺動脈起始),
第72回日本産科婦人科学会学術集会, 2020年4月. 前田崇彰, 西村正人, 祖川英至, 吉田あつ子, 米谷直人, 河北貴子, 香川智洋, 阿部彰子, 加地剛, 苛原稔 :
胎盤病理検査を契機に診断・治療に至った子宮体癌の1例,
第72回日本産科婦人科学会学術集会, 2020年4月. 本間友佳子, 早渕康信, 香美祥二, 加地剛, 苛原稔 :
胎児期に心室瘤の所見を呈した3例,
第30回徳島小児循環器談話会, 2019年9月. 岩佐武, 松崎利也, 阿部彰子, 柳原理江, 山本由理, 香川智洋, 峯田あゆか, 西村正人, 桑原章, 苛原稔 :
子宮内膜異型増殖症・初期子宮体癌患者に対する黄体ホルモン療法の治療成績，妊娠成績および周産期予後,
第72回中国四国日本産科婦人科学会学術講演会, 2019年9月. 本間友佳子, 早渕康信, 香美祥二, 加地剛, 苛原稔 :
胎児期に心室瘤を疑わせる所見を呈した3例,
第37回中国四国地区小児循環器談話会, 2019年9月. 阿部彰子, 井本逸勢, 香川智洋, 峯田あゆか, 西村正人, 苛原稔 :
上皮性卵巣癌に対する遺伝学的検査への意識調査とHRD遺伝子変異の検討,
第61回日本婦人科腫瘍学会, 2019年7月. 加地剛, 早渕康信, 中川竜二, 稲村昇, 苛原稔 :
胎児診断により出生直後の左腕頭動脈肺動脈起始から左腕頭動脈孤立症への経過を観察できた1例,
第55回日本小児循環器学会総会・学術集会, 2019年6月. 西村正人, 峯田あゆか, 阿部彰子, 吉田加奈子, 加藤剛志, 苛原稔 :
若年におけるHPVワクチン接種は子宮頸癌検診における細胞診異常の頻度を低下させる,
第60回日本臨床細胞学会学術講演会, 2019年6月. 加地剛, 早渕康信, 中川竜二, 祖川英至, 七條あつ子, 米谷直人, 須賀健一, 稲村昇, 苛原稔 :
胎児診断により出生直後の左腕頭動脈肺動脈起始から左腕頭動脈孤立症への経過を観察できた1例,
第30回日本心エコー図学会・学術集会, 2019年5月. 岩佐武, 山本由理, 阿部彰子, 桑原章, 西村正人, 松崎敏也, 苛原稔 :
子宮内膜異型増殖症・初期子宮体癌患者に対する黄体ホルモン療法の治療成績，妊娠成績および周産期予後,
第10回日本がん・生殖医療学会, 2019年2月. 松井寿美佳, 安井敏之, 上村浩一, 苛原稔 :
周閉経期におけるコルチゾールの変化と脂質代謝との関連,
第33回日本女性医学学会学術集会, 2018年11月. 松井寿美佳, 安井敏之, 上村浩一, 苛原稔 :
周閉経期のFSHのばらつきとBMIとの関連,
第33回日本女性医学学会学術集会, 2018年11月. 吉田加奈子, 門田友里, 笠井可菜, 河北貴子, 加藤剛志, 苛原稔 :
妊娠中の腹腔鏡下卵巣腫瘍手術におけるアプローチ法の工夫,
日本女性医学学会雑誌, Vol.26, 2018年10月. 森本雅美, 宮本容子, 吉田友紀子, 大津雅信, 池内真由美, 中川美砂子, 阿部彰子, 鳥羽博明, 武知浩和, 桑原章, 井本逸勢, 苛原稔, 丹黒章 :
当院における遺伝性腫瘍に対する取り組み∼Li-Fraumeni症候群を経験して∼,
第15回日本乳癌学会中国四国地方会, 2018年9月. 加地剛, 早渕康信, 須賀健一, 中川竜二, 苛原稔 :
出生後に高度気管狭窄症状を呈した左肺動脈右肺動脈起始(PA sling)の一例,
第54回日本小児循環器学会総会・学術集会, 2018年7月. 吉本夏実, 加地剛, 早渕康信, 今泉絢貴, 七條あつ子, 米谷直人, 須賀健一, 中川竜二, 苛原稔 :
出生後に高度気管狭窄症状を呈した左肺動脈右肺動脈起始(PA sling)の一例,
第91回日本超音波医学会学術集会, 2018年6月. 松井寿美佳, 安井敏之, 上村浩一, 苛原稔 :
副腎アンドロゲンはホルモン補充療法中，わずかなエストロゲンの増加でも低下する,
第70回日本産科婦人科学会学術講演会, 2018年5月. 松井寿美佳, 安井敏之, 上村浩一, 苛原稔 :
周閉経期女性におけるコルチゾールの変化と脂質代謝との関連,
第91回日本内分泌学会学術総会, 2018年4月. Kanako Yoshida, Kadota Yuri, Kasai Kana, Kawakita Takako, Takeshi Katou and Minoru Irahara :
Healthcare for young gynecologic malignant tumor patients at our hospital,
Acta Obstetrica et Gynaecologica Japonica, Vol.70, No.2, 637, Feb. 2018. 吉田加奈子, 門田友里, 笠井可菜, 河北貴子, 加藤剛志, 苛原稔 :
妊娠中の腹腔鏡下卵巣腫瘍手術におけるアプローチ法の工夫,
日本産科婦人科内視鏡学会雑誌, Vol.34, 2018年. 苛原稔 :
教育講演: 遺伝診療に必要な研究倫理,
第3回日本産科婦人科遺伝診療学会学術講演会, 2017年12月. 苛原稔 :
特別講演: 産婦人科医が知っておきたい生殖医療と乳癌検診の話題,
尼崎市産婦人科医会学術講演会, 2017年11月. 田山貴広, 市原裕子, 渡辺典子, 須賀健一, 中川竜二, 香美祥二, 加地剛, 苛原稔 :
肺動脈スリングを合併した先天性気管狭窄症の1例,
第69回中国四国小児科学会, 2017年11月. 苛原稔 :
特別講演:産婦人科領域における乳がん検診,
第94回明石市・加古川市・高砂市産婦人科医会学術講演会, 2017年11月. 七條あつ子, 加地剛, 米谷直人, 苛原稔, 中川竜二 :
当院における精神疾患合併妊娠の検討,
第112回徳島周産期研究会, 2017年11月. 苛原稔 :
特別講演: これからの生殖医療の展望と課題,
平成29年度第4回福岡県産婦人科医会福岡ブロック会学術講演会, 2017年11月. 苛原稔 :
会長講演: 女性ヘルスケアからみた乳がん検診の意義,
第27回日本乳癌検診学会学術集会, 2017年11月. 松井寿美佳, 安井敏之, 笠井可菜, 吉田加奈子, 加藤剛志, 上村浩一, 苛原稔 :
ホルモン補充療法中，デヒドロエピアンドロステロンサルフェートは，わずかなエストロゲンの増加でも低下する,
第32回日本女性医学学会学術集会, 2017年11月. 苛原稔 :
ランチョンセミナー: これからの生殖内分泌学を考える,
第22回日本生殖内分泌学会学術集会, 2017年9月. 竹内麻由美, 松崎健司, 西村正人, 原田雅史, 苛原稔 :
付属器腫瘤の鑑別における''preserved follicle sign''の臨床的有用性について,
JSAWI (The Japanese Society for the Advancement of Women's Imaging) 第15回シンポジウム, 2017年9月. 苛原稔 :
招聘講演: 「日本産科婦人科学会からの提言」これからの生殖医療に求められる期待と責務,
第35回日本受精着床学会総会・学術講演会, 2017年7月. 市原裕子, 須賀健一, 中川竜二, 米谷直人, 苛原稔 :
早産と正期産の妊婦における血清ビオチン濃度の検討,
第53回日本周産期・新生児学会学術集会, 2017年7月. 加地剛, 早渕康信, 北川哲也, 苛原稔 :
診断時には左心低形成症候群への進行の予測が困難であった胎児大動脈弁狭窄の1例,
第53回日本小児循環器学会総会・学術集会, 2017年7月. 加地剛, 七條あつ子, 米谷直人, 早渕康信, 前田和寿, 苛原稔 :
胎児心室中隔欠損における欠損部位の違いによる短絡血流の差異,
第90回日本超音波医学会・学術集会, 2017年5月. 苛原稔 :
特別講演:日常診療に役立つ産婦人科の知識,
第1回徳島市医師会学術講演会, 2017年5月. 吉田守美子, 黒田暁生, 苛原稔, 倉橋清衛, 近藤剛史, 遠藤逸朗, 粟飯原賢一, 松久宗英 :
加重型妊娠高血圧腎症を来し集学的治療で分娩に至った糖尿病腎症の2症例,
第6回臨床高血圧フォーラム, 2017年5月. 苛原稔 :
特別講演:生殖医療の現状とこれからの展望,
平成29年度島根産科婦人科学会・島根県産婦人科医会集会, 2017年4月. 苛原稔 :
特別講演: 生殖医療の現状と課題, --- 性ホルモン製剤のトッピックスを含めて ---,
奈良県産婦人科医会学術講演会, 2017年4月. 苛原稔 :
特別講演: 産婦人科領域における性ホルモン製剤の最近の話題,
徳島県病院薬剤師会学術講演会, 2017年4月. 苛原稔 :
特別講演:生殖医療の現状と今後の在り方,
第10回「生殖と女性医学」講演会, 2017年4月. 山本清成, 轟貴史, 大櫛祐一郎, 上野理恵, 伊勢孝之, 瀬野弘光, 西條良仁, 高木恵理, 原知也, 川端豊, 斎藤友子, 伊藤浩敬, 松浦朋美, 飛梅威, 楠瀬賢也, 山口浩司, 八木秀介, 山田博胤, 添木武, 若槻哲三, 赤池雅史, 佐田政隆, 門田友里, 西村正人, 苛原稔 :
PET/CTにて診断できた心サルコイドーシスの1例,
第254回徳島医学会学術集会, 2017年2月. 苛原稔 :
シンポジウム:HBOCへの対応のために産婦人科で必要なこと,
第5回日本HBOCコンソーシアム学術総会, 2017年1月. 大隅敬太, 早渕康信, 小野朱美, 市原裕子, 木下ゆき子, 東田好広, 漆原真樹, 香美祥二, 須賀健一, 中川竜二, 菅野幹雄, 北川哲也, 加地剛, 苛原稔, 井本逸勢 :
心臓腫瘍の診断を契機に胎児期から管理し，多臓器における系統的診療をすすめた結節性硬化症の一例,
第147回日本小児科学会徳島地方会, 2016年12月. 苛原稔 :
教育セミナー:生殖医療が直面する倫理問題を考える,
第31回日本生殖免疫学会総会・学術集会, 2016年12月. 加地剛, 早渕康信, 北川哲也, 苛原稔 :
胎児完全大血管転位症におけるカラードプラ超音波を用いた冠動脈走行評価,
第52回日本小児循環器学会総会・学術集会, 2016年7月. 苛原稔 :
特別講演:これからの日本の生殖医療の役割とその展望,
第15回日本不妊カウンセリング学会学術集会, 2016年6月. 苛原稔 :
教育講演:NIPTの課題と今後の検査体制について,
第1回日本産科婦人遺伝診療学会学術講演会, 2015年12月. 苛原稔 :
講義:不妊症・不育症とは,
平成27年度不妊・不育相談支援研修, 2015年11月. 毛山薫, 安井敏之, 笠井可菜, 松井寿美佳, 加藤剛志, 上村浩一, 苛原稔 :
閉経期女性における脂質代謝と男性ホルモン,
第30回日本女性医学学会学術集会, 2015年11月. 松井寿美佳, 安井敏之, 笠井可菜, 毛山薫, 加藤剛志, 上村浩一, 苛原稔 :
エストロゲンの投与ルートによる男性ホルモンの変化の違いについての検討,
第30回日本女性医学学会学術集会, 2015年11月. 林亜紀, 吉田守美子, 黒田暁生, 七篠あつ子, 苛原稔, 鶴尾美穂, 森本佳奈, 倉橋清衛, 近藤剛史, 田蒔基行, 明比祐子, 遠藤逸朗, 船木真理, 松久宗英, 福本誠二, 粟飯原賢一, 安倍正博 :
分娩前に10g/日以上の蛋白尿を合併し，出産に至った糖尿病腎症の1例,
日本糖尿病学会中国四国地方会第53回総会, 2015年10月. 苛原稔 :
倫理シンポジウム: 医学研究・臨床試験の不正防止について,
第53回日本癌治療学会学術集会, 2015年10月. 毛山薫, 松井寿美佳, 安井敏之, 笠井可菜, 加藤剛志, 上村浩一, 苛原稔 :
周閉経期女性における脂質代謝と男性ホルモン,
第68中国四国産科婦人科学会総会ならびに学術講演会, 2015年9月. 乾宏彰, 加地剛, 七條あつ子, 髙橋洋平, 中山聡一朗, 早渕康信, 香美祥二, 苛原稔, 遠藤聡子 :
妊娠19週から経過を見ることができた胎児大動脈弁狭窄から左心低形成症候群への進行例,
第68回中国四国産科婦人科学会総会ならびに学術講演, 2015年9月. 竹内麻由美, 松崎健司, 原田雅史, 苛原稔 :
婦人科領域におけるreduced FOV DWIの有用性について,
JSAWI (The Japanese Society for the Advancement of Women's Imaging) 第16回シンポジウム, 2015年9月. 苛原稔 :
特別講演:女性ヘルスケアの観点からみた少子晩産化時代の乳がん検診,
第23回日本がん検診・診断学会総会，第24回日本婦人科がん検診学会総会・学術講演会, 2015年8月. 乾宏彰, 加地剛, 七條あつ子, 髙橋洋平, 中山聡一朗, 苛原稔, 早渕康信, 香美祥二, 遠藤聡子 :
胎児期に大動脈弁狭窄から左心低形成症候群に進行した1例,
第107回徳島周産期研究会, 2015年5月. 加地剛, 七條あつ子, 髙橋洋平, 中山聡一朗, 前田和寿, 苛原稔, 早渕康信, 香美祥二 :
完全大血管転位における冠動脈走行の出生前評価,
第21回日本胎児心臓病学会学術集会, 2015年2月. 松井寿美佳, 安井敏之, 毛山薫, 加藤剛志, 上村浩一, 苛原稔 :
動脈硬化度に対する超低用量ホルモン補充療法の効果についての検討,
第29回日本女性医学学会学術集会, 2014年11月. 苛原稔 :
ランチョンセミナー:女性ヘルスケアのための知っておきたいホルモン療法の最新知識,
第41回日本産婦人科医会学術集会, 2014年10月. 松井寿美佳, 安井敏之, 毛山薫, 加藤剛志, 上村浩一, 苛原稔 :
超低用量ホルモン補充療法は動脈硬化度を改善する,
第67回中国四国産科婦人科学会総会ならびに学術講演会, 2014年9月. 竹内麻由美, 松崎健司, 原田雅史, 苛原稔 :
女性骨盤部領域における磁化率強調像の有用性について,
JSAWI (The Japanese Society for the Advancement of Women's Imaging) 第15回シンポジウム, 2014年9月. 苛原稔 :
特別講演: 子宮内膜症の疼痛管理と不妊治療, --- 内分泌療法の有用性 ---,
第6回新潟子宮内膜症研究会, 2014年5月. 加地剛, 前田和寿, 髙橋洋平, 中山聡一朗, 苛原稔 :
ミニワークショップ:Dual Dopplerを使った胎児右心室の等容拡張時間および等容収縮時間の測定,
第66回日本産科婦人科学会学術講演会, 2014年4月. 苛原稔 :
基調講演: 産婦人科診療における乳癌検診の重要性,
第2回関西生殖医学集談会・第46回関西アンドロロジーカンファレンス, 2014年3月. 菅野幹雄, 菅澤典子, 黒部裕嗣, 中山泰介, 吉田誉, 北市隆, 木下肇, 神原保, 藤本鋭貴, 苛原稔, 北川哲也 :
臍帯血細胞移植は肺高血圧を改善させるか,
第44回日本心臓血管外科学会学術総会, 2014年2月. 谷杏奈, 安井敏之, 毛山薫, 松井寿美佳, 加藤剛志, 上村浩一, 苛原稔 :
P2-31-1 周閉経期において血中monocyte chemoattractant prtein-1とインターロイキン-8は異なる動態を示す(Group 69 更年期・老年期・閉経1,一般演題,第66回学術講演会),
日本産科婦人科学会雑誌, Vol.66, No.2, 663, 2014年2月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1540854195319047168

(CiNii: 1540854195319047168) 松井寿美佳, 安井敏之, 毛山薫, 谷杏奈, 加藤剛志, 上村浩一, 苛原稔 :
P2-31-3 閉経後早期の血中スクレロスチンは閉経前よりも低い(Group 69 更年期・老年期・閉経1,一般演題,第66回学術講演会),
日本産科婦人科学会雑誌, Vol.66, No.2, 663, 2014年2月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1544231895039575168

(CiNii: 1544231895039575168) 加藤剛志, 毛山薫, 河北貴子, 谷杏奈, 松井寿美佳, 吉田加奈子, 西村正人, 上村浩一, 安井敏之, 苛原稔 :
P2-31-8 当院におけるがん患者に対するHRT施行の現状(Group 69 更年期・老年期・閉経1,一般演題,第66回学術講演会),
日本産科婦人科学会雑誌, Vol.66, No.2, 665, 2014年2月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1541698620249180800

(CiNii: 1541698620249180800) 苛原稔 :
イブニングセミナー:子宮内膜症とホルモン療法∼最近の動向∼,
第7回日本性差医学・医療学会学術集会, 2014年1月. 苛原稔 :
ランチョンセミナー:視床下部・下垂体からのゴナドトロピン分泌調節の最近の話題,
第28回日本生殖免疫学会総会・学術集会, 2013年11月. 山本藍美, 田中優, 山川祥悟, 桑原章, 田中保, 苛原稔, 德村彰 :
ラット卵胞発育および排卵調節機構におけるリゾホスファチジン酸受容体と代謝酵素の関与,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, プログラム, 2013年10月. 中川竜二, 西條隆彦, 加地剛, 中山聡一朗, 苛原稔 :
完全気管輪を伴った右肺欠損の低出生体重児の管理,
第104回徳島周産期研究会, 2013年10月. 苛原稔 :
特別講演:生殖補助医療30年の発展とこれからの課題,
平成25年度喜和会総会・学術講演会, 2013年9月. 竹内麻由美, 松崎健司, 原田雅史, 西村正人, 苛原稔 :
子宮膿瘍のMR Spectroscopy,
JSAWI (The Japanese Society for the Advancement of Women's Imaging) 第14回シンポジウム, 2013年9月. 苛原稔 :
特別講演:子宮内膜症における疼痛対策と不妊治療,
第24回近畿エンドメトリオーシス研究会, 2013年8月. 苛原稔 :
指定講演:日本生殖医学会の現状と未来・専門医制度のあり方について,
第49回中国四国生殖医学会学術講演会, 2013年8月. 苛原稔 :
ランチョンセミナー:ART難治症例における卵巣刺激法を含めた総合戦略,
第31回日本受精着床学会総会, 2013年8月. 苛原稔 :
特別講演: 我が国の生殖補助医療の発展とこれからの課題,
札幌東豊セミナー, 2013年8月. 苛原稔 :
特別講演:子宮内膜症に対するホルモン療法の留意点,
札幌市産婦人科医会学術講演会, 2013年7月. 苛原稔 :
ランチョンセミナー: 産婦人科領域における女性ヘルスケア管理の重要性,
第125回関東連合産科婦人科学会総会・学術集会, 2013年6月. 苛原稔 :
シンポジウム:助産実践の創造に向けて助産師にもとめるもの, --- – 医師の立場から - ---,
第69回日本助産師学会, 2013年5月. 苛原稔 :
教育講演:産婦人科診療における乳癌検診の重要性,
産婦人科診療における乳癌検診の重要性, 2013年5月. 苛原稔 :
ランチョンセミナー: 我が国におけるARTの現状 – 使用薬剤・管理方法・成績の解析,
第65回日本産科婦人科学会学術集会, 2013年5月. 苛原稔 :
産婦人科における乳がん検診と画像診断,
第10回愛知県産婦人科医会学術研修会並びに第9回産婦人科MEセミナー(東海), 2013年3月. 苛原稔 :
子宮内膜症に対する内分泌療法の特徴と選択指針,
一土会講演会, 2013年2月. E Takagi, R Ota, 仁木敏之, M Kadota, Y Kawabata, T Hara, A Takashima, 坂東美佳, S Bando, T Matsuura, 伊勢孝之, J Hotchi, 上田由佳, 山口浩司, 八木秀介, 岩瀬俊, 竹谷善雄, N Tomita, 山田博胤, 添木武, 若槻哲三, 佐田政隆, 中山聡一朗, 前田和寿, 苛原稔 :
妊娠を契機にATⅢ欠損症と診断され，血栓管理の後に出産に至った一例,
第246回徳島医学会学術集会(平成24年度冬期), 2013年2月. 苛原稔 :
多嚢胞性卵巣症候群と生活習慣病,
下関産婦人科医会学術講演会, 2013年1月. 苛原稔 :
ライフステージから見たウイメンズヘルスケア, --- ホルモン治療と女性癌予防の視点から ---,
Women's Seminar 2013, 2013年1月. 苛原稔 :
男の更年期，女の更年期,
新春講演会, 2013年1月. 谷杏奈, 山本哲史, 松井寿美佳, 加藤剛志, 國見幸太郎, 上村浩一, 安井敏之, 前川正彦, 鎌田正晴, 苛原稔 :
若年子宮内膜症患者では動脈硬化が進んでいる,
第27回日本生殖免疫学会会員・学術集会, 2012年12月. 宮本健志, 溝渕佳史, 影治照喜, 永廣信治, 小谷裕美子, 香美祥二, 加地剛, 苛原稔 :
胎児期に発見された頭蓋咽頭腫の一例,
第74回㈳日本脳神経外科学会中国四国支部学術集会, 2012年12月. 苛原稔 :
更年期以降の女性のヘルスケア,
香川三知会, 2012年11月. 苛原稔 :
ランチョンセミナー:不妊治療による多胎妊娠の最近の動向と予防法,
第57回日本生殖医学学術講演会, 2012年11月. 谷杏奈, 山本哲史, 松井寿美佳, 加藤剛志, 國見幸太郎, 上村浩一, 安井敏之, 苛原稔 :
若年子宮内膜症患者では動脈硬化が進んでいる,
第27回日本女性医学学会学術集会, 2012年10月. 松井寿美佳, 安井敏之, 谷杏奈, 加藤剛志, 國見幸太郎, 上村浩一, 苛原稔 :
周閉経期におけるアディポネクチンの動態にテストステロンが関与する,
第27回日本女性医学学会学術集会, 2012年10月. 苛原稔 :
シンポジウム: 生涯にわたる女性の血圧管理, --- 月経異常とメタボリック症候群 ---,
第27回日本女性医学学会学術集会, 2012年10月. 苛原稔 :
ホルモン補充療法の乳癌リスクの最近の動向,
徳島県病院薬剤師学術講演会, 2012年10月. 苛原稔 :
内分泌療法の選択と進め方,
子宮内膜症セミナー, 2012年10月. 中山聡一朗, 安井敏之, 加地剛, 上村浩一, 松井寿美佳, 前田和寿, 苛原稔 :
双胎妊娠と単胎妊娠の母体骨代謝の比較,
第65回中国四国産科婦人科学会総会ならびに学術講演会, 2012年9月. 谷杏奈, 山本哲史, 松井寿美佳, 加藤剛志, 國見幸太郎, 上村浩一, 安井敏之, 苛原稔 :
若年子宮内膜症患者は動脈硬化が進んでいる,
第65回中国四国産科婦人科学会総会ならびに学術講演会, 2012年9月. 竹内麻由美, 松崎健司, 原田雅史, 古本博孝, 苛原稔 :
卵巣腺線維腫・嚢胞腺線維腫のMRI診断,
JSAWI (The Japanese Society for the Advancement of Women's Imaging) 第13回シンポジウム, 2012年9月. 苛原稔 :
講義: 治療総論・検査・診断,
第1回日本生殖医学会生殖医療従事者講習会, 2012年9月. 苛原稔 :
子宮内膜症の内分泌療法の選択と進め方,
第63回広島産科婦人科学会総会, 2012年9月. 苛原稔 :
「ART 基礎シリーズ講義」生殖と内分泌環境,
第30回日本受精着床学会総会•学術講演会, 2012年8月. 田中優, 松崎利也, 苛原稔 :
シンポジウム「PSOSとインスリン抵抗性」, --- PCOSにおけるテストステロンとインスリン抵抗性の評価に関する検討 ---,
第36回日本産科婦人科栄養代謝研究会, 2012年8月. 苛原稔 :
HRTと乳癌リスク, --- HRTガイドラインの改定を受けて ---,
東豊セミナー, 2012年8月. 吉田加奈子, 加藤剛志, 西村正人, 古本博孝, 苛原稔 :
シンポジウム:子宮頸癌における腺系異常, --- 各領域における腺系腫瘍診断の工夫と最新技術 ---,
第27回日本臨床細胞学会中国四国連合会総会・学術集会, 2012年7月. 苛原稔 :
子宮内膜症内分泌療法としてのジェノゲストの有用性,
全国ディナゲスト研究会, 2012年7月. 苛原稔 :
中高年女性の健康管理のための最新知識, --- 更年期障害治療から疾病予防まで ---,
JA高知病院公開カンファレンス, 2012年7月. 竹下茂樹, 上村浩一, 寺本勝寛, 菊谷真理子, 竹村秀雄, 赤川元, 森田哲夫, 瀬戸裕, 井上裕子, 土橋一慶, 苛原稔, 日本産婦人科乳癌学会研修委員会 :
妊婦乳がんスクリーニング prospective studyへの検討,
第20回日本乳癌学会学術総会, 2012年6月. 西村正人, 古本博孝, 吉田加奈子, 苛原稔 :
支部長推薦演題:幽門腺化生∼悪性腺腫の頸管粘液中の胃型ムチンと細胞像,
第53回日本臨床細胞学会(春期大会), 2012年6月. 苛原稔 :
産婦人科医療と漢方,
第6回四国漢方セミナー, 2012年5月. 加地剛, 前田和寿, 七條あつ子, 河見貴子, 中山聡一朗, 佐藤美紀, 苛原稔 :
ワークショップ:皆で共有したい印象に残る産婦人科症例, --- 胎児超音波検査にて特異な大腿骨像を呈した1例 ---,
第85回日本超音波医学会, 2012年5月. 苛原稔 :
女性のライフステージからみたPCOSの管理,
千葉県産科婦人科学会平成24年度定例総会•学術講演会, 2012年5月. 岩佐武, 松崎利也, 苛原稔 :
シンポジウム: GnIH，キスペプチン/メタスチンによる性腺機能調節, --- 胎生期の低栄養がキスペプチン/メタスチンに及ぼす影響 ---,
第85回日本内分泌学会学術総会, 2012年4月. 中山聡一朗, 安井敏之, 加地剛, 松井寿美佳, 佐藤美紀, 上村浩一, 前田和寿, 苛原稔 :
多胎妊娠と単胎妊娠における妊娠産褥期の骨代謝の比較,
第64回日本産科婦人科学会学術講演会, 2012年4月. 松井寿美佳, 安井敏之, 谷杏奈, 國見幸太郎, 上村浩一, 山本哲史, 苛原稔 :
Gonadotropin-releasing hormone agonist役与によるインスリン抵抗性の変化,
第64回日本産科婦人科学会学術講演会, 2012年4月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1543950420061985408

(CiNii: 1543950420061985408) 苛原稔 :
子宮頸がん予防とHPVワクチンの新しい展開,
第1回徳島市医師会学術講演会, 2012年4月. 苛原稔 :
女性のライフステージからみた乳がん検診,
第18回日本産婦人科乳癌学会, 2012年3月. 苛原稔 :
シンポジウム:大学卒前教育から卒直後教育までの一貫性ある漢方医学教育を目指して,
KAMPO MEDICAL SYMPOSIUM 2012, 2012年2月. 大西達也, 早渕康信, 阪田美穂, 苛原誠, 香美祥二, 加地剛, 前田和寿, 苛原稔 :
無脾症候群・下心臓型肺静脈還流異常に対する静脈管ステント留置部病変の経胸壁・血管内エコーによる経時的観察,
第23回日本Pediatric Interventional Cardiology学会学術集会, 2012年1月. 田中優, 松崎利也, 苛原稔 :
シンポジウム: 病来生理に基づいたPCOS治療戦略, --- PCOSの病態生理と診断基準 ---,
第56回日本生殖医学会学術講演会, 2011年12月. 笠井由佳, 前川正彦, 谷杏奈, 松井寿美佳, 國見幸太郎, 山本哲史, 上村浩一, 安井敏之, 鎌田正晴, 苛原稔 :
ラロキシフェンが末梢血全血のサイトカイン産生に及ぼす効果．,
第26回日本女性医学学会学術集会, 2011年11月. 國見幸太郎, 松井寿美佳, 谷杏奈, 上村浩一, 山本哲史, 安井敏之, 苛原稔 :
骨盤臓器脱治療におけるQOL評価．,
第26回日本女性医学学会学術集会, 2011年11月. 谷杏奈, 安井敏之, 松井寿美佳, 國見幸太郎, 上村浩一, 山本哲史, 苛原稔 :
GnRH agonist投与によって血中monocyte chemoattractant protein-1は増加する,
第26回日本女性医学学会学術集会, 2011年11月. 中山総一朗, 安井敏之, 上村浩一, 松井寿美佳, 谷杏奈, 佐藤美紀, 加地剛, 國見幸太郎, 山本哲史, 前田和寿, 苛原稔 :
双胎妊娠と単胎妊娠における妊娠産褥期の骨代謝の比較．,
第26回日本女性医学学会学術集会, 2011年11月. 松井寿美佳, 安井敏之, 谷杏奈, 國見幸太郎, 上村浩一, 山本哲史, 苛原稔 :
Gonadotropin-releasing hormone agonist投与がインスリン抵抗性に与える影響,
第26回日本女性医学学会学術集会, 2011年11月. 苛原稔 :
女性医学と乳腺疾患管理,
第26回日本女性医学学会学術集会, 2011年11月. 苛原稔 :
ワークショップ: 産婦人科医と乳癌検診の関わり, --- 不妊症と乳癌検診 ---,
第21回日本乳癌検診学会学術集会, 2011年10月. 苛原稔 :
PCOS:最近の新知見とARTのための排卵誘発法,
第29回日本受精着床学会総会•学術集会, 2011年9月. 吉田加奈子, 東元あゆか, 阿部彰子, 海老沢桂子, 加藤剛志, 西村正人, 古本博孝, 苛原稔 :
ワークショップ: 子宮体癌新FIGO分類pTla期に対するリンパ節郭清の必要性について,
第50回日本婦人科腫瘍学会学術講演会, 2011年7月. 大西達也, 早渕康信, 阪田美穂, 苛原誠, 香美祥二, 渡辺典子, 中川竜二, 西條隆彦, 加地剛, 前田和寿, 苛原稔, 菅野幹雄, 北市隆, 北川哲也 :
静脈管ステント留置術を行った無脾症に伴う下心臓型総肺静脈還流異常症の新生児例,
第136回日本小児科学会徳島地方会, 2011年6月. 中川竜二, 西條隆彦, 須賀健一, 渡辺典子, 大西達也, 阪田美穂, 早渕康信, 香美祥二, 加地剛, 前田和寿, 苛原稔 :
生後早期に静脈管ステント留置術を行った無脾症に伴う下心臓型総肺静脈環流異常症の一例,
第28回四国新生児医療研究会, 2011年5月. 大西達也, 早渕康信, 阪田美穂, 苛原誠, 香美祥二, 加地剛, 前田和寿, 苛原稔, 菅野幹雄, 北市隆, 北川哲也 :
下心臓型総肺静脈還流異常に対して静脈管にステント留置を施行した1新生児例,
第98回日本循環器学会中国四国合同地方会, 2011年5月. 松崎利也, 岩佐武, 苛原稔 :
シンポジウム:生殖機能の中枢性調節:最近の知見, --- 出生前後の栄養状態およびストレスが成長後の性機能に及ぼす影響 ---,
第84回日本内分泌学会学術総会, 2011年4月. 松井寿美佳, 安井敏之, 上村浩一, 山本哲史, 苛原稔 :
GnRH agonistの投与によって血中monocyte chemoattractant protein-1は増加する,
第63回日本産科婦人科学会学術講演会, 2011年4月. 加地剛, 前田和寿, 須藤真功, 佐藤美紀, 中川竜二, 西條隆彦, 苛原稔, 早渕康信, 香美祥二 :
当院における胎児心エコー検査 -保険収載後1年を経て-,
第99回徳島周産期研究会, 2011年4月. 苛原稔 :
子宮内膜症に対する内分泌療法, --- ∼性ステロイドホルモンの有用性∼ ---,
周南産婦人科フォーラム, 2011年2月. 苛原稔 :
婦人科でできること,
第90回指導者のための避妊と性感染症予防セミナー, 2011年2月. 苛原稔 :
女性のライフサイクルマネージメントにおける婦人科医の役割,
香川県産婦人科医会・日本産科婦人科学会香川地方部会合同研修会学術講演会, 2010年12月. 中川竜二, 西條隆彦, 香美祥二, 前田和寿, 苛原稔 :
本県における新生児蘇生法普及事業の現状,
第135回日本小児科学会徳島地方会, 2010年12月. 苛原稔 :
婦人科的視点から見た乳がん薬物療法の管理,
第8回日本乳癌学会近畿地方会, 2010年12月. 笠原善郎, 辻一郎, 大内憲明, 東野英利子, 横江隆夫, 苛原稔, 丹黒章 :
乳癌検診の不利益:マンモグラフィ検診偽陽性例の実態調査,
第20回日本乳癌検診学会総会, 2010年11月. 苛原稔 :
子宮頸がんにならないために, --- –検診とHPVワクチン- ---,
女性のためのがんセミナー in安城2010, 2010年10月. 苛原稔 :
機能性出血の病態に基づく対応,
第37回日本産婦人科医会学術集会創立60周年記念大会, 2010年10月. 上村浩一, 松井寿美佳, 山本哲史, 安井敏之, 苛原稔 :
閉経周辺期女性の血管脈派伝播速度に影響を及ぼす因子の検討,
第25回日本更年期医学会学術集会, 2010年10月. 苛原稔 :
乳がん診療における産婦人科医の役割,
第5回中四国乳房超音波研究会, 2010年9月. 前田和寿, 須藤真功, 佐藤美紀, 加地剛, 苛原稔 :
糖尿病治療の最前線, --- 妊娠糖尿病の診断と管理 ---,
第10回日本内分泌学会四国支部学術集会, 2010年9月. 苛原稔 :
女性医学とその将来と展望,
東豊セミナー, 2010年8月. 苛原稔 :
産婦人科疾患における漢方治療の有用性,
第33回日本東洋医学会徳島県部会平成22年度前期学術集会, 2010年7月. 苛原稔 :
HRTの最近の話題, --- ∼ガイドラインを中心に∼ ---,
宮崎県北地区産婦人科医会(二八会), 2010年7月. 苛原稔 :
子宮内膜症に対する内分泌療法, --- ∼性ステロイドホルモンの有用性∼ ---,
福岡ディナゲスト研究会, 2010年7月. 苛原稔 :
多嚢胞性卵巣症候群の診断と治療 Update,
第122回近畿産婦人科学会総会ならびに学術集会, 2010年6月. 苛原稔 :
子宮内膜症の薬物治療 Update, --- ∼子宮内膜症取扱い規約の改定を受けて∼ ---,
北九州産婦人科医会学術講演会, 2010年6月. 苛原稔 :
ライフステージに応じたホルモン製剤の適正使用,
平成22年度女性のQQL向上を支援する医師とコメディカルのための女性医療セミナー, 2010年6月. 加地剛, 早渕康信, 須藤真功, 佐藤美紀, 中川竜二, 西條隆彦, 前田和寿, 苛原稔, 香美祥二 :
右上大静脈欠損を伴った左上大上静脈左房還流の胎児診断例,
第83回日本超音波医学会学術集会, 2010年5月. 苛原稔 :
生殖医療の新しい展開, --- 日本産科婦人科学会子宮内膜症取り扱い規約改訂を受けて ---,
市川市産婦人科医会研修会, 2010年5月. 苛原稔 :
子宮内膜症の内分泌療法の新しい展開, --- -日産婦学会子宮内膜症取扱い規約の改定をうけて- ---,
子宮内膜セミナー, 2010年5月. 松井寿美佳, 安井敏之, 西條礼子, 上村浩一, 山本哲史, 苛原稔 :
周閉経期においてNT-proBNPはSHBGと相関する,
第62回日本産科婦人科学会学術講演会, 2010年4月. 苛原稔 :
子宮頸がんとHPVワクチン,
東四国医療セミナー, 2010年4月. 松井寿美佳, 安井敏之, 西條礼子, 上村浩一, 苛原稔 :
周閉経期女性においてNT-proBNPは性ホルモン結合グロブリンと相関する,
第24回日本更年期医学会学術集会, 2009年10月. 上村浩一, 安井敏之, 松井寿美佳, 西條礼子, 苛原稔 :
日本の中高年婦人において，血清クレアチニン低値と軽度耐糖能障害との関連についての検討,
第24回日本更年期医学会学術集会, 2009年10月. 安井敏之, 上村浩一, 苛原稔, 新井信, 岡部竜吾, 小島原典子, 田代眞一, 佐藤弘 :
加齢とともに変化する内因性性ホルモンは脂質・糖代謝に影響するか,
第9回日本抗加齢医学会総会, 2009年5月. 西條礼子, 安井敏之, 上村浩一, 苛原稔 :
ラロキシフェン投与による血中monocyte chemoattractant protein-1およびインターロイキン7の変化,
第82回日本内分泌学会学術総会, 2009年4月. 安井敏之, 西條礼子, 上村浩一, 苛原稔 :
ラロキシフェンの投与によって血中monocyte chemoattractant protein-1およびインターロイキン7は減少する,
第61回日本産科婦人科学会総会・学術講演会, 2009年4月. 上村浩一, 安井敏之, 西條礼子, 苛原稔 :
閉経後婦人のメタボリック症候群の有病状況の予測因子としての血中γGTP濃度の有用性の検討,
第23回日本更年期医学会, 2008年11月. 西條礼子, 安井敏之, 宮谷友香, 上村浩一, 苛原稔 :
閉経期女性におけるアディポネクチンとサイトカインの検討,
第23回日本更年期医学会, 2008年11月. 安井敏之, 宮谷友香, 山田正代, 上村浩一, 苛原稔 :
閉経周辺期女性におけるアデイポネクチンとサイトカインの検討,
第60回日本産科婦人科学会学術講演会および総会, 2008年4月. 上村浩一, 安井敏之, 宮谷友香, 山田正代, 日吉峰麗, 有澤孝吉, 苛原稔 :
閉経後女性に対するビスフォスフォネート投与時の反応性と骨代謝関連因子の検討,
第7回徳島Bone Forum, 2007年11月. 山田正代, 安井敏之, 上村浩一, 譲原光利, 野口将道, 土屋直子, 加藤義夫, 苛原稔 :
精神神経症状を有する更年期女性における選択的セロトニン再取込み阻害剤および加味逍遙散によるサイトカインの変化,
第22回日本更年期医学会学術集会, 2007年11月. 安井敏之, 宮谷友香, 山田正代, 上村浩一, 苛原稔, 新井信, 小島原典子, 岡部竜吾, 石井康智, 田代眞一, 佐藤弘 :
高齢男女においてアデイポネクチンと性ステロイドホルモン結合グロブリンは相関する,
第22回日本更年期医学会学術集会, 2007年11月. 上村浩一, 安井敏之, 山田正代, 苛原稔 :
閉経後の骨量減少症および骨粗鬆症に対するビスフォスフォネート単剤治療における反応不良および反応鈍化の要因の検討,
第22回日本更年期医学会学術集会, 2007年11月. 川添和義, 水口和生, 苛原稔 :
健康食品，漢方薬，そして薬, --- 「補完代替医療・おくすり相談室」の事例から学ぶこと ---,
第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 31, 2007年11月. 坂根亜由子, 三好淳, 田中滋康, 松崎利也, 苛原稔, 石崎宏好, 岡本三紀, 高井義美, 佐々木卓也 :
Rab3低分子量G蛋白質ファミリーによる視床下部-下垂体-性腺系の制御,
第80回日本内分泌学会学術総会, 2007年6月. 宮谷友香, 安井敏之, 冨田純子, 山田正代, 上村浩一, 苛原稔 :
血中アデイポネクチンは高齢男女とも性ステロイドホルモン結合グロブリンと相関する,
第59回日本産科婦人科学会総会・学術講演会, 2007年4月. 安井敏之, 冨田純子, 宮谷友香, 山田正代, 上村浩一, 前川正彦, 苛原稔 :
閉経周辺期および両側卵巣摘出後女性におけるホットフラッシュとサイトカインに関する検討,
第59回日本産科婦人科学会総会・学術講演会, 2007年4月. 加地剛, 安井敏之, 須藤真功, 三谷龍史, 上村浩一, 前田和寿, 苛原稔 :
切迫早産における安静加療は骨代謝に影響を与える,
第59回日本産科婦人科学会総会・学術講演会, 2007年4月. 加地剛, 森一博, 鈴木光二郎, 須藤真功, 中山聡一郎, 三谷龍史, 中川竜二, 早渕康信, 前田和寿, 西條隆彦, 苛原稔, 香美祥二 :
総肺静脈還流異常(下心臓型)に静脈管無形成を伴った内臓錯位症候群の一例,
第18回日本心エコー図学会学術集会, 2007年4月. 上村浩一, 安井敏之, 加地剛, 冨田純子, 宮谷友香, 山田正代, 須藤真功, 三谷龍史, 前田和寿, 苛原稔 :
妊娠中の切迫早産によるベッド上安静が母体の骨代謝に与える影響,
第13回徳島骨代謝研究会, 2006年11月. 冨田純子, 安井敏之, 宮谷友香, 山田正代, 上村浩一, 前川正彦, 土屋直子, 譲原光利, 竹田秀一, 苛原稔 :

第21回日本更年期学会学術集会, 2006年10月. 山田正代, 安井敏之, 冨田純子, 宮谷友香, 上村浩一, 前川正彦, 土屋直子, 譲原光利, 竹田秀一, 苛原稔 :
閉経周辺期および両側卵巣摘出後女性におけるホットフラッシュとサイトカインに関する検討,
第21回日本更年期学会学術集会, 2006年10月. 上村浩一, 安井敏之, 冨田純子, 宮谷友香, 山田正代, 苛原稔 :
閉経後婦人の血中osteoprotegerin濃度と心血管病変の危険因子との関係の検討,
第21回日本更年期学会学術集会, 2006年10月. 上村浩一, 山田正代, 安井敏之, 森本忠興, 苛原稔 :
当科における乳腺外来の現状と課題,
第9回日本産婦人科乳癌学会, 2006年9月. 笠井由佳, 前川正彦, 小林文子, 海野友香, 山田正代, 上村浩一, 安井敏之, 鎌田正晴, 苛原稔 :
ラロキシフェンが単球由来のサイトカイン産生に及ぼす影響について -エストラジオールとの比較検討,
第2回SERM学術研究会学術集会, 2006年6月. 冨田純子, 安井敏之, 海野友香, 山田正代, 上村浩一, 苛原稔, 三浦雅一 :
結合型エストロゲンを用いたホルモン補充療法による非カルボキシル化オステオカルシンと中性脂肪の検討,
第59回日本産科婦人科学会総会・学術講演会, 2006年4月. 海野友香, 安井敏之, 冨田純子, 山田正代, 上村浩一, 苛原稔 :
高齢者における血中テストステロン濃度とbody mass indexとの関係には性差がある,
第59回日本産科婦人科学会総会・学術講演会, 2006年4月. 安井敏之, 山田正代, 海野友香, 冨田純子, 上村浩一, 前川正彦, 苛原稔 :
閉経周辺期女性におけるサイトカインの動態,
第59回日本産科婦人科学会総会・学術講演会, 2006年4月. 笠井由佳, 前川正彦, 小林文子, 海野友香, 山田正代, 上村浩一, 安井敏之, 鎌田正晴, 苛原稔 :
ラロキシフェンが単球由来のサイトカイン産生に及ぼす影響についてエストラジオールとの比較検討,
第59回日本産科婦人科学会総会・学術講演会, 2006年4月. 加地剛, 森一博, 阪田美穂, 森根幹生, 中川竜二, 早渕康信, 前田和寿, 西條隆彦, 苛原稔, 香美祥二 :
妊娠14週から胎内経過を追うことができたEbstein奇形の1例,
第12回日本胎児心臓病研究会, 2006年2月. 坂本久美子, 日比野有紀, 木原勝, 山野修司, 前田和寿, 苛原稔, 山内あい子 :
徳島大学病院における妊婦に対する処方医薬品とその胎児危険度評価,
第15回日本医療薬学会年会, 2005年10月. 日比野有紀, 坂本久美子, 木原勝, 山内あい子, 山野修司, 前田和寿, 苛原稔 :
徳島大学病院における妊婦に対する医薬品使用状況調査,
日本薬学会第125年会, 2005年3月. 桑原章, 森川富昭, 苛原稔, 森口博基 :
生殖補助医療(ART)に対する多施設間情報共有システムの開発,
医療情報学24回連合大会論文集, 598-599, 2004年11月. 上村浩一, 安井敏之, 海野友香, 山田正代, 苛原稔 :
閉経後婦人の可溶型破骨細胞分化因子(soluble RANKL)と破骨細胞分化抑制因子(osteoprotegerin)の血中動態に関する検討,
第15回婦人科骨粗鬆症研究会, 2004年11月. 上村浩一, 安井敏之, 海野友香, 山田正代, 苛原稔 :
閉経後婦人のosteoprotegerin(OPG)とフリーおよびOPG結合型可溶性RANKLの血中動態に関する検討,
第19回日本更年期医学会学術集会, 2004年10月. 上村浩一, 安井敏之, 海野友香, 滝川雅也, 苛原稔 :
HRTおよびビスフォスフォネート投与による血中osteoprotegerinおよびsoluble RANKL濃度の変化の検討,
第56回日本産科婦人科学会総会ならびに学術講演会, 2004年4月. 上村浩一, 安井敏之, 海野友香, 滝川雅也, 苛原稔 :
骨量減少·骨粗鬆症患者に対するHRTやビスフォスフォネート投与による血中OPG, soluble RANKL濃度の変化の検討,
第18回日本更年期医学会学術集会, 2003年11月. 上村浩一, 安井敏之, 海野友香, 滝川稚也, 苛原稔 :
血中エストロゲン濃度の変動にともなう血中osteoprotegerin濃度の推移の検討,
第76回日本内分泌学会学術総会, 2003年5月. 上村浩一, 安井敏之, 滝川雅也, 苛原稔 :
血中エストロゲン濃度の変動による血中osteoprotegerin(OPG)濃度の推移の検討,
第13回婦人科骨粗鬆症研究会, 2002年11月. 上村浩一, 安井敏之, 海野友香, 滝川雅也, 苛原稔 :
血中エストロゲン濃度の短期·長期的な変動にともなう血中osteoprotegerin濃度の推移の検討,
第17回日本更年期医学会学術集会, 2002年10月. 上村浩一, 安井敏之, 古本博孝, 苛原稔 :
当科における乳房外来の現状,
第55回日本産科婦人科学会中国四国合同地方部会総会·学術集会, 2002年9月. 上村浩一, 田中優, 三浦尚子, 尾形理江, 清川麻知子, 桑原章, 井川洋, 松崎利也, 安井敏之, 苛原稔 :
血中エストロゲン濃度の変動による血中osteoprotegerin(OPG)濃度の推移および骨代謝との関係の検討,
第54回日本産科婦人科学会総会ならびに学術講演会, 2002年4月. 上村浩一, 清川麻知子, 安井敏之, 苛原稔, 青野敏博 :
結合型エストロゲン投与による血中osteoprotegerin(OPG)濃度の推移および骨密度，骨代謝との関係の検討,
第16回日本更年期医学会学術集会, 2001年11月. 上村浩一, 清川麻知子, 安井敏之, 苛原稔 :
血中エストロゲン濃度の変動による血中osteoprotegerin(OPG)濃度の推移および骨代謝との関係の検討,
第12回婦人科骨粗鬆症研究会, 2001年10月. 上村浩一, 安井敏之, 古本博孝, 苛原稔 :
当科における乳房外来の現状,
第54回日本産科婦人科学会中国四国合同地方部会総会ならびに学術講演会, 2001年9月. 上村浩一, 手束典子, 山田正代, 安井敏之, 苛原稔, 青野敏博 :
妊娠·産褥期の血中osteoprotegerin (OPG)/osteoclastgenesis inhibitory factor(OCIF)濃度の推移および骨代謝との関係の検討,
第53回日本産科婦人科学会総会ならびに学術講演会, 2001年5月. 上村浩一, 手束典子, 山田正代, 安井敏之, 苛原稔, 青野敏博 :
妊娠·産褥期の血中 osteoprotegerin (OPG)/osteoclastgenesis inhibitory factor (OCIF) 濃度の推移および骨代謝との関係の検討,
第11回婦人科骨粗鬆症研究会, 2000年11月.

研究会・報告書: 河北貴子, 加藤剛志, 門田友里, 笠井可菜, 吉田加奈子, 苛原稔 :
International Session Workshop : New mouse model of uterine adenomyosis.,
第72回日本産科婦人科学会学術講演会, 2021年4月. 吉田あつ子, 加地剛, 祖川英至, 米谷直人, 須賀健一, 中川竜二, 苛原稔 :
男性ホルモン投与中に自然妊娠し女児を出産したFemale to Maleの一例,
徳島産婦人科医報, No.52, 38, 2019年10月. 須賀健一, 中川竜二, 吉田あつ子, 米谷直人, 加地剛, 苛原稔, 庄野実希, 香美祥二 :
当院周産母子センターの過去5年間での在胎30週未満の早産児の臨床データ解析全国周産期医療母子医療センターネットワークデータとの比較,
徳島産婦人科医報, No.52, 39, 2019年10月. 加地剛, 早渕康信, 中川竜二, 稲村昇, 苛原稔 :
胎児診断により出生直後の左腕頭動脈肺動脈起始から左腕頭動脈孤立症への経過を観察できた1例,
日本小児循環器学会雑誌, Vol.35, No.Suppl.1, 394, 2019年6月. 吉本夏実, 加地剛, 吉田あつ子, 祖川英至, 米谷直人, 市原裕子, 須賀健一, 中川竜二, 石橋広樹, 苛原稔 :
胎児超音波にて直腸腟前庭瘻を描出できた鎖肛の一例,
超音波医学, Vol.46, No.Suppl., S695, 2019年4月. 須賀健一, 中川竜二, 吉田あつ子, 米谷直人, 加地剛, 苛原稔, 庄野実希, 香美祥二 :
院周産母子センターの過去5年間での在胎30週未満の早産児の臨床データ解析~全国周産期母子医療センターネットワークデータとの比較~,
第151会日本小児科学会徳島地方会, 2018年12月. 七條あつ子, 加地剛, 米谷直人, 中川竜二, 苛原稔 :
当院における精神疾患合併妊娠の検討,
徳島産婦人科医報, No.51, 34, 2018年8月. 吉田あつ子, 加地剛, 今泉絢貴, 米谷直人, 中川竜二, 苛原稔 :
男性ホルモン投与中に自然妊娠し，女児を出産したFemale to Maleの一例,
日本周産期・新生児医学会雑誌, Vol.54, No.2, 611, 2018年6月. 武田明日香, 加地剛, 今泉絢貴, 七條あつ子, 米谷直人, 市原裕子, 須賀健一, 中川竜二, 苛原稔 :
出生後に高度気管狭窄症状を呈した左肺動脈右肺動脈起始(PA sling)の一例,
日本周産期・新生児医学会雑誌, Vol.54, No.2, 697, 2018年6月. 吉本夏実, 加地剛, 早渕康信, 今泉絢貴, 吉田あつ子, 米谷直人, 須賀健一, 中川竜二, 苛原稔 :
出生後に高度気管狭窄症状を呈した左肺動脈右肺動脈起始(PA sling)の一例,
超音波医学, Vol.45, 845, 2018年4月. 苛原稔 :
特別講演: HRTガイドラインの改訂とこれからの展望,
北海道産婦人科医会学術研修会, 2018年3月. 苛原稔 :
特別講演: 生殖医療の現状とこれからの展望,
拡大一土会講演会, 2018年2月. 苛原稔 :
特別講演:産婦人科医療が直面する遺伝診療の諸問題,
札幌東豊セミナー, 2018年2月. 苛原稔 :
特別講演: 女性ヘルスケアからみた乳がん診療へのアドバイス,
平成29年度乳がん検診講習会, 2017年11月. 市原裕子, 渡辺典子, 須賀健一, 中川竜二, 香美祥二, 七條あつ子, 加地剛, 米谷直人, 苛原稔 :
Townes-Brocks症候群が疑われる多発奇形の1例,
第112回徳島周産期研究会, 2017年11月. 田山貴広, 市原裕子, 渡辺典子, 須賀健一, 中川竜二, 香美祥二, 加地剛, 苛原稔 :
栄養ドリンクが原因と考えられた胎児動脈管早期収縮の1例,
第112回徳島周産期研究会, 2017年11月. 苛原稔 :
特別講演: HBOCに関連する最新の医学的知見の普及,
Ovarian Cancer Science Exchange Meeting in Tokushima, 2017年10月. 苛原稔 :
講義: 治療総論・検査・診断/一般治療各論(排卵誘発),
2017(平成29)年度第1回生殖医療従事者講習会, 2017年8月. 苛原稔 :
特別講演:発展する生殖医療とこれからの課題,
第19回大阪不妊の集い勉強会, 2017年7月. 苛原稔 :
特別講演:専門医から見た生殖医療に関する必要な情報とは, --- 最新の知見を含めて ---,
「不妊治療」セミナー, 2017年6月. 加地剛, 七條あつ子, 米谷直人, 苛原稔, 須賀健一, 中川竜二, 石橋広樹 :
鎖肛の胎児超音波所見についての検討,
第111回徳島周産期研究会, 2017年5月. 苛原稔 :
特別講演: 臨床現場に必要な医学倫理指針と利益相反の最近の考え方,
札幌東豊セミナー, 2017年2月. 苛原稔 :
特別講演:生殖医療の現状とこれからの展望,
第6回愛知産婦人科研究会, 2016年12月. 苛原稔 :
セミナー:岐路に立つ生殖医療, --- その現状と課題 ---,
名古屋大学大学院医学系研究科, 2016年12月. 苛原稔 :
特別講演:女性ライフサイクルマネージメントのためのホルモン治療戦略,
第324回筑後ブロック産婦人科医会研修会, 2016年11月. 苛原稔 :
特別講演: 岐路に立つ生殖医療を考える,
平成28年度慶應義塾大学医学部産婦人科学教室同窓会学術講演会, 2016年11月. 苛原稔 :
特別講演: 生殖医療2016 up date,
平成28年度秋の日産婦医会・社保・運営合同委員会特別講演会, 2016年11月. 苛原稔 :
特別講演: 多嚢胞性卵巣症候群とメタボリックシンドローム,
第6回プライマリーケアセミナー, 2016年11月. 苛原稔 :
講演: 女性のライフステージから『産む』を考える,
日本助産師会中国・四国地区研修会, 2016年10月. 苛原稔 :
特別講演:女性医学におけるホルモン補充療法の役割を考える,
第135回信州産婦人科連合会学術講演会, 2016年9月. 苛原稔 :
講演: 生殖医療への遺伝子診断の応用と問題点,
札幌東豊セミナー, 2016年8月. 苛原稔 :
基調講演: 日本専門医機構基準に則した新たな専門医制度の構築と本会のあり方について,
第38回中部生殖医学会学術集会, 2016年6月. 苛原稔 :
イブニングセミナー:岐路に立つ生殖補助医療, --- その現状と展望 ---,
第52回北陸生殖医学会学術総会, 2016年6月. 苛原稔 :
ランチョンセミナー:女性ヘルスケアにおけるホルモン補充療法の有用性,
第64回北日本産科婦人科学会総会学術講演会, 2016年6月. 苛原稔 :
学術講演: 女性のライフステージに合わせたPCOSの管理,
西宮市・芦屋市産婦人科医会合同学術講演会, 2016年5月. 苛原稔 :
特別講演:女性医学におけるホルモン補充療法の役割と乳癌検診,
第325回岡山四水会, 2016年4月. 苛原稔 :
特別講演:不妊治療の現状と問題点,
第452回徳島県中部臨床研究会, 2016年1月. 苛原稔 :
特別講演:生殖補助医療の現状と将来への課題,
平成27年度富山県産科婦人科学会第5回例会・特別講演会, 2016年1月. 苛原稔 :
教育講演:子宮内膜症の治療について,
子宮内膜症フォーラムin 香川, 2015年12月. 苛原稔 :
特別講演:生殖内分泌学の最近の話題,
第8回三重県産婦人科内分泌研究会, 2015年10月. 苛原稔 :
特別講演:更年期以降女性の医療におけるホルモン補充療法の役割を考える,
札幌東豊セミナー, 2015年8月. 苛原稔 :
公開シンポジウム:産婦人科領域における遺伝診療の最近の話題,
第251回徳島医学会学術集会, 2015年8月. 苛原稔 :
記念講演:不妊症診療と生殖補助医療,
不育症研究センターキックオフ・セミナー, 2015年7月. 苛原稔 :
特別講演:女性ヘルスケアにおける乳腺疾患管理の重要性とこれからの展開,
北海道産婦人科乳腺医学会第6回学術集会, 2015年7月. 苛原稔 :
特別講演:ホルモン補充療法と乳癌リスクに関する最近の考え方,
平成27年度岐阜産科婦人科学会・岐阜県産婦人科医会合同学術研修会, 2015年6月. 苛原稔 :
特別講演:日本におけるARTの現状とこれからの課題,
第1回東海生殖周産期研究会, 2015年3月. 苛原稔 :
特別講演:ホルモン補充療法の意義と留意点に関する最近の考え方,
第9回大分女性医学フォーラム, 2015年2月. 苛原稔 :
セミナー: 代理懐胎を考える,
「女性の健康週間2015」プレスセミナー, 2015年1月. 苛原稔 :
基調講演: 内分泌領域の最新知識の普及,
第20回徳島内分泌研究会, 2014年12月. 苛原稔 :
特別講演:ホルモン補充療法の意義と留意点に関する最近の考え方,
平成26年山梨産婦人科学会・山梨県産婦人科医会並びに合同講演会, 2014年12月. 苛原稔 :
学術講演: 女性ヘルスケアのためのホルモン療法の最近の話題,
京都産婦人科医会11月学術研修会, 2014年11月. 苛原稔 :
特別講演:産婦人科領域からの乳癌管理へのアドバイス,
第27回宮崎乳腺疾患研究会, 2014年10月. 苛原稔 :
特別講演:高齢化少産時代の生殖医療の問題点,
第46回徳島母性衛生学会, 2014年9月. 苛原稔 :
特別講演: 子宮内膜症に対するホルモン療法の留意点,
東予産婦人科医会, 2014年9月. 苛原稔 :
講義: 「治療総論・検査・診断」，「一般治療各論Ⅰ(排卵誘発)」,
平成26年度第1回日本生殖医学会生殖医療従事者講習会, 2014年8月. 苛原稔 :
特別講演: 知っておきたい思春期教育の最新知識, --- 思春期から伝えておきたい健康課題とは ---,
教育関係者のための思秋期教育セミナー(徳島保健所主催), 2014年8月. 苛原稔 :
特別講演:分岐点に立つ生殖医療,
札幌東豊セミナー, 2014年8月. 苛原稔 :
HRTと乳がん検診,
第1回産婦人科医のための乳がん検診参画に向けての講習会, 2014年6月. 苛原稔 :
特別講演:HRTと乳がん検診,
第1回産婦人科医の為の乳がん検診参画に向けての講習会, 2014年6月. 苛原稔 :
特別講演: ホルモン補充療法の意義と留意点,
三重県産婦人科医会特別研修会, 2014年4月. 苛原稔 :
学術講演:ホルモン補充療法の意義と留意点,
愛媛県産婦人科医会学術講演会, 2014年3月. 苛原稔 :
特別講演:子宮内膜症と不妊治療, --- ∼内分泌療法からARTまで∼ ---,
第19回鳥取生殖医学セミナー, 2014年3月. 苛原稔 :
妊娠と不妊の最近の話題,
「不妊に悩む方への特定治療支援事業」の制度改正に係る説明会, 2014年3月. 苛原稔 :
特別講演: 生殖補助医療の現状とこれからの動向,
第13回秋田県生殖・内分泌研究会, 2014年3月. 苛原稔 :
医学研究COIマネージメントガイドラインについて, --- 全国医学部長病院長会議ガイドライン ---,
日本医学会COIマネージメント研修セミナー, 2014年2月. 苛原稔 :
特別講演:産婦人科医による乳がん検診のすすめ, --- ∼画像診断の最近の動向∼ ---,
第16回産婦人科MEセミナー(西日本), 2014年2月. 苛原稔 :
特別講演:これからの思春期教育, --- ∼今，伝えておきたい``からだ''のこと∼ ---,
平成25年度地域保健関係職員研修会, 2014年1月. 苛原稔 :
特別講演: ARTの現状とこれからの課題,
岡山ARTフォーラム2014・冬, 2014年1月. 苛原稔 :
スポンサードレクチャー:女性ヘルスケアからみたPCOSの管理,
第368回東京産科婦人科学会例会, 2013年12月. 寺田知正, 中川竜二, 渡辺典子, 西條隆彦, 近藤朝美, 香美祥二, 加地剛, 中山聡一朗, 苛原稔, 石橋広樹, 矢田圭吾 :
完全気管輪を伴った右肺欠損の低出生体重児の管理,
第141回日本小児科学会徳島地方会, 2013年12月. 苛原稔 :
特別講演:子宮内膜症に対するホルモン療法の留意点,
平成25年度香川県産婦人科医会臨時総会香川産科婦人科学会報告会並びに合同学術講演会, 2013年12月. 苛原稔 :
女性の80%が経験する月経痛の真実(月経困難症·子宮内膜症とは)∼・痛いのガマンしちゃダメ!月経痛は見えない病気のサイン-月経困難症，子宮内膜症とは?- ・一緒に治そう-治療(薬物療法と手術)- ・月経痛専用の治療薬って知ってる?-早期治療の重要性-」,
日本子宮内膜症啓発会議第2回メディアセミナー, 2013年11月. 苛原稔 :
特別講演:ホルモン補充療法の意義と留意点,
熊本更年期・加齢医学フォーラム, 2013年11月. 苛原稔 :
特別講演:子宮内膜症に対するホルモン療法の留意点,
第304回筑後ブロック産婦人科医会研修会, 2013年11月. 苛原稔 :
シンポジウム:医系大学・研究機関・病院における利益相反(COI)マネージメントガイドラインについて,
第4回日本医学会分科会利益相反会議, 2013年11月. 苛原稔 :
特別講演:子宮内膜症に対するホルモン療法の留意点,
平成25年度岐阜産科婦人科研究会, 2013年11月. 苛原稔 :
特別講演:子宮内膜症における疼痛対策と不妊治療,
香川県子宮内膜症フォーラム, 2013年11月. 苛原稔 :
特別講演:不妊症·不育症,
不妊症·不育症講演会, 2013年10月. 苛原稔 :
特別講演: 女性キャリアのためのライフプラニング講座, --- ∼知っておきたい``からだ''のこと∼ ---,
徳島保健所主催, 2013年10月. 苛原稔 :
市民公開講座: 中高年女性の性ホルモン補充を考える,
第9回日本抗加齢医学会北海道研究会, 2013年9月. 苛原稔 :
講義: 「治療総論・検査・診断」，「一般治療各論Ⅰ(排卵誘発)」,
平成25年度第1回日本生殖医学会生殖医療従事者講習会, 2013年8月. 苛原稔 :
私がいちばん好きな処方,
漢方と診療, Vol.3, No.1, 2012年3月. 苛原稔 :
妊娠から母乳育児まで,
健やかな赤ちゃんをむかえるために改訂版, 2012年3月. 苛原稔 :
座談会「これからの女性医学の国際的情勢を見据えて日本での第5回APMF学術集会開催を考える」,
日本女性医学学会雑誌, No.2, 2012年2月. 苛原稔 :
子宮内膜症の内分泌療法up-to-date,
OG SCOPE, No.1, 3-6, 2012年1月. 吉村泰典, 苛原稔, 森本義晴 :
「巻頭解説」In vitroにおける精子形成法の確立, --- 単一胚移植の現状と生産率向上への模索 ---,
ART WorldWide perspective, Vol.22, 3-6, 2012年1月. 鎌田正晴, 炬口友里, 山田正代, 岡田真澄, 横山裕司, 漆川敬治, 前川正彦, 苛原稔 :
〈エキスパートレクチャー3:女性医学と乳癌検診〉乳がん検診,
日本女性医学学会雑誌, No.1, 131-133, 2012年1月. 苛原稔 :
第26回日本女性医学学会学術集会を終えて,
日本女性医学学会誌, No.1, 231, 2012年1月. 苛原稔 :
〈会長講演〉女性医学と乳腺疾患管理,
日本女性医学学会雑誌, No.1, 120-123, 2012年1月. 苛原稔 :
ホルモン補充療法の最近の話題,
徳島県病院薬剤師会学術集会, 2011年12月. 苛原稔 :
子宮頸がんとHPVワクチン,
板野郡医師会学術講演会, 2011年12月. 前田和寿, 中山聡一朗, 佐藤美紀, 加地剛, 苛原稔 :
シンポジウム: 産科的出血と輸血療法,
第44回中国四国医学検査学会, 2011年11月. 苛原稔 :
不妊診療における検査のトピックスと胚培養士の役割,
第44回中国四国医学検査学会, 2011年11月. 苛原稔 :
子宮内膜症治療における内分泌療法の有用性,
福山ディナゲスト研究会, 2011年9月. 苛原稔 :
子宮内膜症治療における内分泌療法の有用性,
岡山子宮内膜症フォーラム, 2011年9月. 苛原稔 :
性ステロイドホルモン製剤とその使い方の最新の進歩,
東豊セミナー, 2011年8月. 西村正人, 古本博孝, 東元あゆか, 吉田加奈子, 苛原稔 :
シンポジウム: 幽門腺化生∼悪性腺腫の頸管粘液中の胃型ムチンと細胞像,
第26回日本臨床細胞学会中国四国連合会学術集会, 2011年7月. 苛原稔 :
女性ホルモン療法と乳癌,
長崎県医師会日医生涯教育協力講座セミナー, 2011年7月. 苛原稔 :
子宮頸癌とHPVワクチン,
東四国医療セミナー, 2011年5月. 苛原稔 :
産婦人科医への女性医学のすすめ,
三知会高知支部会, 2011年5月. 苛原稔 :
これからの地域医療のあり方について,
鳴門市医療フォーラム, 2011年3月. 苛原稔 :
女性における婦人科医の役割,
第282回青森県臨床産婦人科医会, 2011年1月. 苛原稔 :
子宮内膜症に対する内分泌療法, --- —性ステロイドホルモンの有用性- ---,
第6回島根不妊・内分泌研究会, 2010年11月. 苛原稔 :
女性のライフサイクルマネージメントにおける婦人科医の役割,
第288回筑後ブロック産婦人科医会研修会, 2010年11月. 吉村泰典, 苛原稔, 西井修, 百枝幹雄 :
ゴナドトロピンの低用量漸増法による排卵誘発,
産科と婦人科, Vol.77, No.11, 1355-1362, 2010年10月.

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 多嚢胞性卵巣症候群の血中性腺ホルモンとAMHを用いた診断精度の向上に関する研究 (研究課題/領域番号: 18K09293 )
胎生期の低栄養と出生後の栄養環境が生殖機能に及ぼす長期的影響とその機序の解明 (研究課題/領域番号: 18K09292 )
出生後早期に受けた各種ストレスが成長後の性機能に及ぼす長期的影響に関する研究 (研究課題/領域番号: 18K09264 )
周閉経期における中枢・卵巣・副腎ネットワークの時間的調節機構の解明 (研究課題/領域番号: 16K11143 )
視床下部機能低下時の新規中枢神経ペプチドアナログによる新しい排卵誘発法の基礎検討 (研究課題/領域番号: 15K10673 )
多嚢胞性卵巣症候群の診断基準におけるAMHとインスリン抵抗性の意義に関する研究 (研究課題/領域番号: 15K10672 )
卵巣機能の低下が中枢・末梢のストレス反応に及ぼす影響とその機序の検討 (研究課題/領域番号: 26462526 )
周閉経期女性における男性ホルモンの果たす役割についての検討 (研究課題/領域番号: 25462596 )
中枢神経ペプチドを利用した新しい排卵誘発法の基礎検討 (研究課題/領域番号: 24592473 )
多嚢胞性卵巣症候群患者に対する治療法の個別化に関する内分泌学的検討 (研究課題/領域番号: 24592472 )
周閉経期の内分泌・免疫学的変化が閉経後の代謝に与える時間的影響についての検討 (研究課題/領域番号: 22591857 )
新規脳内ペプチドによるゴナドトロピン分泌調節機構と各種ストレスによる影響の検討 (研究課題/領域番号: 21592100 )
多嚢胞性卵巣症候群の診断精度の向上に関する検討 (研究課題/領域番号: 20591951 )
安全性と至便性を兼ね備えた新しい排卵誘発法の確立に関する研究 (研究課題/領域番号: 17591744 )
新しく発見された摂食調節因子の性機能へのクロストークの解明 (研究課題/領域番号: 14571566 )
排卵機構における卵巣局所調節因子に関する研究 (研究課題/領域番号: 13671722 )
精子抗原ペプチド発現微生物を用いた遺伝子経粘膜ワクチンの開発 (研究課題/領域番号: 12557137 )
卵胞閉鎖機構におけるアポトーシスの意義およびその調節に関する研究 (研究課題/領域番号: 10671548 )
ゴナドトロピンによる排卵誘発の適正治療法の確立に関する研究 (研究課題/領域番号: 10557148 )
副甲状腺ホルモン関連蛋白(PTHrP)の生理学的機構の解明 (研究課題/領域番号: 07671789 )
ヒトの単一卵胞発育の機序の解明と排卵誘発時の多胎妊娠予防に関する研究 (研究課題/領域番号: 07457389 )
多嚢胞卵巣症候群の診断技術の向上と治療法の改善に関する研究 (研究課題/領域番号: 04454421 )
内因性摂食調節物質の視床下部ー下垂体ー卵巣系に及ぼす影響に関する研究 (研究課題/領域番号: 02670743 )
体外受精・胚移植における妊婦率向上に関する研究 (研究課題/領域番号: 01480395 )
免疫性不妊症における糖鎖抗原の意義および治療法の開発に関する研究 (研究課題/領域番号: 62570759 )
ゴナドトロピン及びプロラクチン分泌に関する細胞内機構の解明 (研究課題/領域番号: 60480367 )
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所属学会・所属協会: 日本産科婦人科学会
日本不妊学会
日本更年期学会
社団法人日本内分泌学会
日本生殖内分泌学会
日本生殖免疫学会
委員歴・役員歴: 日本産科婦人科学会 (代議員)
日本不妊学会 (理事)
日本更年期学会 (理事)
社団法人日本内分泌学会 (代議員)
日本生殖内分泌学会 (理事)
日本生殖免疫学会 (評議員)
受賞: 2010年10月, The best imaging賞 (第20回日本超音波医学会四国地方学術集会)
2011年2月, 里見賞「研究部門」 (第17回日本胎児心臓病学会学術集会)
2015年11月, 学会賞 (日本女性医学学会)
活動: 徳島県健康対策審議会 (委員)
徳島県総合健診センター (理事)

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Jグローバル最終確認日: 2024/4/13 01:18
氏名（漢字）: 苛原稔
氏名（フリガナ）: イラハラミノル
氏名（英字）: Irahara Minoru
所属機関: 徳島大学

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researchmap最終確認日: 2024/4/14 01:22
氏名（漢字）: 苛原稔
氏名（フリガナ）: イラハラミノル
氏名（英字）: Irahara Minoru
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登録日時: 2002/8/19 00:00
更新日時: 2002/8/19 00:00
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所属: 徳島大学
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2024年4月13日更新

研究者番号: 20160070

所属（現在）: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 特命教授

所属（過去の研究課題 情報に基づく）*注記: 2019/4/1 – 2020/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 研究部長
2018/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 教授
2016/4/1 – 2017/4/1 : 徳島大学, 大学院医歯薬学研究部(医学系), 教授
2015/4/1 : 徳島大学, 大学院医歯薬学研究部, 教授
2014/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2012/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 教授
2008/4/1 – 2012/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2004/4/1 – 2005/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2001/4/1 – 2003/4/1 : 徳島大学, 医学部, 教授
1999/4/1 – 2000/4/1 : 徳島大学, 医学部・附属病院, 講師
1998/4/1 : 徳島大学, 医学部付属病院, 講師
1998/4/1 : 徳島大学, 医学部附属病院, 講師
1995/4/1 – 1997/4/1 : 徳島大学, 医学部・附属病院, 講師
1996/4/1 : 徳島大学, 医学部, 講師
1994/4/1 : 徳島大学, 医学部・付属病院, 講師
1994/4/1 : 徳島大学, 医学部附属病院, 講師
1992/4/1 – 1993/4/1 : 徳島大学, 医学部・附属病院, 講師
1991/4/1 : 徳島大学, 医学部・付属病院, 講師
1991/4/1 : 徳島大学, 医学部附属病院, 講師
1990/4/1 : 徳島大学, 医学部・付属病院, 助手
1988/4/1 – 1989/4/1 : 徳島大学, 医学部附属病院, 助手
1987/4/1 : 徳島大学, 医学部, 助手
1987/4/1 : 徳島大学, 医学部・付属病院, 助手

審査区分/研究分野

研究代表者

医学 / 外科 / 産婦人科学
生物系 / 医歯薬学 / 外科系臨床医学 / 産婦人科学
小区分56040:産婦人科学関連

研究代表者以外

医学 / 外科 / 産婦人科学
生物系 / 医歯薬学 / 外科系臨床医学 / 産婦人科学
小区分56040:産婦人科学関連

キーワード

研究代表者

内因性摂食調節物質 / 性機能 / 視床下部ー下垂体ー卵巣系 / ゴナドトロピン分泌 / LHパルス状分泌 / 多嚢胞性卵巣症候群 / PCOS / LH / FSH比 / 診断基準 / エクルーシス / テストステロン / アンドロゲン / 診断 / アーキテクト / ホルモン測定系 / 代謝異常 / 生活習慣病 / メトホルミン / インスリン抵抗性 / 排卵誘発 / Interleukin-1β / ICE / サイトカイン / 卵巣 / 卵胞発育 / 卵胞閉鎖 / TNFα / GnRH / Interlenkin-1β / アポトーシス / IL-1β変換酵素 / 排卵機構 / Fasシステム / Interleukin-1 beta / cytokin / ovary / folliclogenesis / follicle atresia / TNF-alph / GRO-CINC / ケモカイン / インターロイキン / 排卵 / ラット / 好中球 / PMSG-hCG / CHEMOKINE / INTERLEUKINE / OVULATION / FOLLICULARATRESIA / 多胎妊娠 / FSH / 自己注射 / ovulation induction / low-dose FSH therapy / FSH-pulsatile GnRH therapy / self injection / metformin / polycystic ovary syndrome / safety / convenience / 受精 / 精子不動化抗体 / 免疫性不妊 / 糖鎖構造 / 卵・精子相互作用 / 卵透明帯精子貫通試験 / レクチン / マンノース / 卵透明帯 / 精子認識機構 / アクロゾーム反応 / 受精阻害 / 卵透明帯貫通試験 / ヒト卵透明帯 / 精子 / 糖鎖 / Fertilization / Sperm immobilizing antibodies / Immunological infertility / Oligosaccharides / Sperm-egg interaction / Sperm penetration test using zona pellucida / Lectin / 抗ミュラー管ホルモン / AMH / 年齢 / 卵巣サイズ

研究代表者以外

体外受精胚移植 / 過排卵誘発法 / 血中ホルモン測定値 / GnRHーhMGーhCG療法 / 体外受精・胚移植 / 血中・ホルモン測定値 / 過排的刺激法 / GnRH analog / 生殖医学 / ゴナドトロピン分泌 / 視床下部 / 神経ペプチド / プログラミング / ストレス / キスペプチン / RFRP / GnIH / 絶食 / kisspeptin / 絶食ストレス / 性機能 / レプチン / ゴナドトロピン / 感染ストレス / 摂食 / 閉経 / 内分泌 / 免疫 / 代謝 / 周閉経期 / エストロゲン / テストステロン / インスリン抵抗性 / 性ステロイドホルモン結合グロブリン / サイトカイン / anti-Mullerian hormone / ペプチド / 排卵誘発 / 排卵障害 / GnRH / 多嚢胞性卵巣症候群 / レトロゾール / senktide / letrozol / 脂質代謝 / 糖代謝 / 男性ホルモン / 脂質・糖代謝 / 肝機能 / DHEA-S / スクレロスチン / アディポネクチン / ホルモン補充療法 / 卵巣 / 脂肪組織 / visfatin / 性腺ホルモン / 心理ストレス / 多嚢胞卵巣症候群 / 臨床症状 / 検査所見 / 診断基準 / 治療 / ゴナドトロピン療法 / GnRHアゴニスト / GuRH律動投与法 / GnRH律動投与法 / 卵巣過剰刺激症候群 / 多胎妊娠 / pureFSH療法 / LH-RHアゴニスト療法 / LHRH律動授与法 / PCOS / Clical complaints / Examination / Criteria for diagnosis / Treatment / GnRH agonist / Ovulation induction / 不妊症 / 排卵誘発法 / 単一卵胞発育 / コナドトロピン療法 / 卵胞発育 / 卵胞刺激ホルモン / 卵胞刺激ホルモン放出ホルモン / 性腺刺激ホルモン放出ホルモン / Sterility / Ovulation Induction / anovulation / Pulsatile gonadtropin-releasing hormone treatment / Gonadotropin treatment / Multiple pregnancy / Single folliculogenesis / Ovarian hyperstimulation syndrome / PTHrP / 乳腺上皮細胞 / プロゲステロン / デキサメサゾン / デキサメタゾン / 乳汁 / Ca / 乳汁分泌量 / 産褥ラット / 初代培養系 / 副甲状腺ホルモン関連蛋白 / 骨代謝 / milk / Progesterone / Dexamethasone / GnRHパルス療法 / FSH低用量維持療法 / 卵巣過剩刺激症候群 / FSH GnRH健動投与 / FSH-GnRH律動投与 / FSH-GnRH律動投与法 / 視床下部性排卵障害 / 避妊ワクチン / 遺伝子ワクチン / 精子抗原 / 抗精子抗体 / 粘膜ワクチン / immunocontraceptive vaccine / DNA vaccine / sperm antigen / peptide / 節食調節因子 / オレキシン / グレリン / LH / レプチン受容体 / 摂食調節因子 / βエンドルフィン / orexinergic peptides / orexin / ghrelin / leptin / reptin receptor / 下垂体前葉細胞 / CTH_3細胞 / プロクラチン / ホスファチジールイノシトール / アラキドン酸 / Cキナーゼ / TRH,LH-RH / プロラクチン / PMA / 5-HETE / Prolactin / Pituitary cells / Calcium / GH_3 cells / Protein kinase C / Phosphatidyl inositol / KNDyニューロン / NKB / ダイノルフィン / センクタイド / nor-BNI / nor-MNI / 副腎 / コルチゾール / DHEAS / 下垂体 / 性成熟 / 性行動 / プロゲステロン受容体 / 出生後早期のストレス / 長期的影響 / アンドロゲン / 肥満 / 栄養代謝 / 胎児期低栄養 / 生殖機能 / やせ / 生殖

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