プラズマ遺伝子導入の機序解明と作用要因のバランス制御による高効率非侵襲化

トップ›研究者を探す›プラズマ遺伝子導入の機序解明と作用要因のバランス制御による高効率非侵襲化

プラズマ遺伝子導入の機序解明と作用要因のバランス制御による高効率非侵襲化

代表研究者	神野雅文
研究課題番号	KAKENHI-PUBLICLY-15H00896
研究種目	新学術領域研究(研究領域提案型)
研究分野
研究機関	愛媛大学
研究期間開始年月日	2015/4/1
研究期間終了年度	2016
研究ステータス	完了 (2016/4/1)
配分額（合計）	11,700,000 (直接経費 :9,000,000、間接経費 :2,700,000)
配分額（履歴）	2016年度：5,850,000 (直接経費 :4,500,000、間接経費 :1,350,000) 2015年度：5,850,000 (直接経費 :4,500,000、間接経費 :1,350,000)
キーワード	プラズマ医療遺伝子導入マイクロプラズマラジカルシナジー効果エンドサイトーシス遺伝子医療育種プラズマプラズマ遺伝子導入法遺伝子形質転換マイクロキャピラリー

研究成果

[学会発表] プラズマ照射による脂質二重膜の変形と分子透過過程の検討

山本健太、永岩秀憲、木戸祐吾、本村英樹、佐藤晋、神野雅文 2017

[学会発表] プラズマ分子導入における活性酸素種の役割の導入分子量依存性

池田善久、磯崎勇希、木戸祐吾、佐藤晋、神野雅文 2017

[雑誌論文] Synergistic effect of electrical and chemical factors on endocytosis in micro‐discharge plasma gene transfection

Masafumi Jinno, Yoshihisa Ikeda, Hideki Motomura, Yuki Isozaki, Yugo Kido, Susumu Satoh 2017

[学会発表] プラズマによるC3H/HeJ マウス皮膚へのin vivo 遺伝子導入

江渕拓也、森貴之、木戸祐吾、池田善久、佐藤晋、神野雅文 2017

[学会発表] プラズマ照射によるタマネギ表皮細胞への蛍光色素導入

西雅明、大野雄貴、木戸祐吾、池田善久、佐藤晋、神野雅文 2017

[学会発表] プラズマ照射によるスマ魚卵への蛍光色素導入

大野雄貴、船越健太、木戸祐吾、池田善久、佐藤晋、神野雅文 2017

[雑誌論文] Reactive oxygen species and intracellular Ca2+ contribution to micro-discharge plasma gene transfection

Yuki Isozaki, Yoshihisa Ikeda, Yugo Kido, Susumu Satoh, Masafumi Jinno 2017

[雑誌論文] Improvement of efficiency and viability in plasma gene transfection by plasma minimization and optimizing electrodes configuration

Masafumi Jinno, Kunihide Tachibana, Hideki Motomura, Noboru Saeki, Susumu Satoh 2016

[雑誌論文] Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection

Masafumi Jinno, Yoshihisa Ikeda, Hideki Motomura,Yugo Kido,Susumu Satoh 2016

[雑誌論文] Effects of molecular size and chemical factor on plasma gene transfection

Yoshihisa Ikeda, Hideki Motomura, Yugo Kido, Susumu Satoh, and Masafumi Jinno 2016

[学会発表] Investigation of cell membrane permeation process in plasma molecule transfection using artificial cell

Hideki Motomura, Daijiro Aibara, Hidenori Aibara, Yugo Kido, Yoshihisa Ikeda, Susumu Satoh, Masafumi Jinno 2016

[学会発表] The synergistic eect between electrical and chemical factors in plasma gene/molecule-transfection

Masafumi Jinno 2016

[雑誌論文] Monte Carlo poration model of cell membranes for application to plasma gene transfection

Amel Zerrouki, Mohammed Yousfi, Ahmed Rhallabi, Hideki Motomura and Masafumi Jinno 2016

[学会発表] プラズマ照射によるメダカ魚卵への蛍光色素導入

池田善久、大野雄貴、西雅明、船越健太、木戸祐吾、斎藤大樹、後藤理恵、松原孝博、本村英樹、佐藤晋、神野雅文 2016

[雑誌論文] Optical emission spectroscopy characterizations of micro-air plasma used for simulation of cell membrane poration

Amel Zerrouki, Hideki Motomura, Yoshihisa Ikeda, Masafumi Jinno, Mohammed Yousfi 2016

[学会発表] Membrane permeabilization induced by microplasma irradiation

Hideki Motomura, Hidenori Nagaiwa, Daijiro Aibara, Yugo Kido, Yoshihisa Ikeda, Susumu Satoh, Masafumi Jinno 2016

[雑誌論文] Effects of molecular size and chemical factor on plasma gene transfection

Yoshihisa Ikeda, Hideki Motomura, Yugo Kido, Susumu Satoh, Masafumi Jinno 2016

[雑誌論文] Improvement of efficiency and viability in plasma gene transfection by plasma minimization and optimization electrode configuration

Masafumi Jinno, Kunihide Tachibana, Hideki Motomura, Noboru Saeki, Susumu Satoh 2016

[学会発表] High-efficient and high-survivability plasma gene transfection by minimizing plasma and optimizing electrodes configuration

Hideki Motomura, Yoshihisa Ikeda, Kunihide Tachibana, Noboru Saeki, Susumu Satoh, Masafumi Jinno 2016

[学会発表] The role of electrical and chemical factors in the molecular/gene transfection by micro-plasma irradiation

Masafumi Jinno, Yoshihisa Ikeda, Hideki Motomura, Yugo Kido, Susumu Satoh 2016

[学会発表] Investigation of discharge propagation on cell and plasmid suspension in plasma gene transfection

Yugo Kido, Hideki Motomura, Yoshihisa Ikeda, Susumu Satoh, Masafumi Jinno 2016

[学会発表] プラズマ照射による脂質二重膜の変形と分子透過過程の検討

永岩秀憲、山本健太、木戸祐吾、池田善久、本村英樹、佐藤晋、神野雅文 2016

[学会発表] 複数の針電極放電によるプラズマ遺伝子導入法

磯崎勇希、大西章仁、木戸祐吾、池田善久、本村英樹、佐藤晋、神野雅文 2016

[学会発表] Cytotoxicity of plasma gene transfection

Yoshihisa Ikeda, Yuki Isozaki, Yugo Kido, Hideki Motomura, Susumu Satoh, Masafumi Jinno 2016

[雑誌論文] Investigation of plasma induced electrical and chemical factors and their contribution processes to plasma gene transfection

Masafumi Jinno, Yoshihisa Ikeda, Hideki Motomura, Yugo Kido, Susumu Satoh 2016

[学会発表] Membrane deformation and permeabilization caused by microplasma irradiation

Hideki Motomura, Hidenori Nagaiwa, Kenta Yamamoto, Yugo Kido, Yoshihisa Ikeda, Susumu Satoh, Masafumi Jinno 2016

[学会発表] 大気圧非平衡プラズマのアプリケーションとしてのプラズマ遺伝子導入

神野雅文 2016

[学会発表] プラズマ遺伝子導入; 何がすごいのか!

神野雅文 2016

[学会発表] Micro-air plasma characterizations from optical emission spectroscopy for gene transfection application

Amel Zerrouki, Hideki Motomura, Yoshihisa Ikeda, Masafumi Jinno, Mohammed Yousfi 2016

[学会発表] Evaluation of importance of H2O2 in gene transfection

Yoshihisa Ikeda, Masanori Kimura, Yuki Isozaki, Yugo Kido, Hideki Motomura, Susumu Satoh, Masafumi Jinno 2016

[学会発表] プラズマ遺伝子導入法～導入機序と必要とされるプラズマ特性の検討

神野雅文 2016

[学会発表] 多種細胞におけるプラズマ遺伝子導入法の導入傾向

中野孝輝、宮本展寛、木戸祐吾、池田善久、本村英樹、佐藤晋、神野雅文 2016

[学会発表] Clathrin-mediated endocytosis contribution to plasma gene transfection

Masafumi Jinno, Yuki Isozaki, Yugo Kido, Yoshihisa Ikeda, Hideki Motomura, Susumu Satoh 2016

[学会発表] Synergistic effect between electrical and chemical factors in plasma gene transfection

Masafumi Jinno, Yuki Isozaki, Ken Miyake, Masanori Kimura, Yugo Kido, Yoshihisa Ikeda, Hideki Motomura, Susumu Satoh 2016

[雑誌論文] プラズマによる遺伝子導入

神野雅文、佐藤晋 2015

[学会発表] Effect of the radical species for gene transfection by discharge plasma irradiation

Y. Ikeda, H. Motomura, Y. Kido, S. Satoh, K. Tachibana and M. Jinno 2015

[学会発表] Contribution of the Reactive Species to the Plasma Gene Transfection

Y. Ikeda, H. Motomura, Y. Kido, S. Satoh, K. Tachibana and M. Jinno 2015

[学会発表] Quantitative Investigation of Surface Charge of Liposome for Analysis of Plasma Gene Transfection Mechanism

H. Motomura, Y. Ikeda, Y. Kido, K. Tachibana, S. Satoh and M. Jinno 2015

[学会発表] Influence of the H2O2 in the plasma gene transfection method

H. Nagaiwa, D. Aibara, Y. Ikeda, H. Motomura, Y. Kido, S. Satoh, K. Tachibana, M. Jinno 2015

[学会発表] Mechanism of Plasma Gene Transfection

M. Jinno, K. Manabe, Y. Isozaki, Y. Ikeda, H. Motomura, Y. Kido, S. Satoh and K. Tachibana 2015

[学会発表] The effect of the suspension cells in plasma gene transfection method

Y. Isozaki, K. Nakano, Y. Ikeda, H. Motomura, Y. Kido, S. Satoh, K. Tachibana and M. Jinno 2015

[学会発表] Effect of the cell temperature in the plasma gene transfection

Y. Ikeda, K. Manabe, Y. Isozaki, H. Motomura, Y. Kido, S. Satoh, K. Tachibana and M. Jinno 2015