研究成果

[雑誌論文] Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device

Nguyen Suong T.、Edo Ayane、Nagahara Megumi、Otoi Takeshige、Taniguchi Masayasu、Takagi Mitsuhiro 2024

[学会発表] Development of porcine embryos cultured in media irradiated with ultraviolet at 228 and 260 nm wavelength.

Torigoe Nanaka, Aihara, M. Lin Qingyi, Takebayashi Koki, Liu Bin, Nagahara Megumi and Otoi Takeshige. 2024

[学会発表] Genome editing of porcine zygotes through the lipofection of a CRISPR/Cas9 system with two guide RNAs.

Lin Qingyi, Takebayashi Koki, Torigoe Nanaka, Liu Bin, Hirata, M., Tanihara, F., Nagahara Megumi and Otoi Takeshige. 2024

[雑誌論文] Effects of ergothioneine supplementation on the quality of liquid-preserved and frozen-thawed boar semen

Kaewma Supitcha、Namula Zhao、Nguyen Suong Thi、Lin Qingyi、Torigoe Nanaka、Liu Bin、Nagahara Megumi、Nii Masahiro、Taniguchi Masayasu、Otoi Takeshige 2024

[雑誌論文] Porcine embryo development and inactivation of microorganisms after ultraviolet-C irradiation at 228?nm

Lin Qingyi、Aihara Mutsumi、Shirai Akihiro、Tanaka Ami、Takebayashi Koki、Yoshimura Naoaki、Torigoe Nanaka、Nagahara Megumi、Minamikawa Takeo、Otoi Takeshige 2023

[学会発表] Culture method and transfection reagent combinations in genome editing by lipofection in pig zygotes.

Lin Qingyi, Takebayashi Koki, Torigoe Nanaka, Liu Bin, Hirata Maki, Nagahara Megumi, Otoi Takeshige. 2023

[雑誌論文] Effects of curcumin supplementation on quality of porcine spermatozoa irradiated with ultraviolet‐C at 228 nm during liquid preservation

Nagahara Megumi、Namula Zhao、Lin Qingyi、Takebayashi Koki、Torigoe Nanaka、Liu Bin、Aihara Mutsumi、Nii Masahiro、Otoi Takeshige 2023

[雑誌論文] Comparison of chemically mediated CRISPR/Cas9 gene editing systems using different nonviral vectors in porcine embryos

Lin Qingyi、Takebayashi Koki、Torigoe Nanaka、Liu Bin、Namula Zhao、Hirata Maki、Tanihara Fuminori、Nagahara Megumi、Otoi Takeshige 2023

[雑誌論文] Efficiency of genetic modification in gene‐knockout sperm‐derived zygotes followed by electroporation of guide RNA targeting the same gene

Takebayashi Koki、Wittayarat Manita、Lin Qingyi、Torigoe Nanaka、Liu Bin、Hirata Maki、Nagahara Megumi、Tanihara Fuminori、Otoi Takeshige 2023

[雑誌論文] Short‐term preservation of porcine zygotes at ambient temperature using a chemically defined medium

Lin Qingyi、Le Quynh Anh、Takebayashi Koki、Hirata Maki、Tanihara Fuminori、Sawamoto Osamu、Kikuchi Takeshi、Otoi Takeshige 2022

[学会発表] Triple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods.

Lin Q, Le Q.A., Wittayarat M, Namula Z, Takebayashi K, Hirata M, Tanihara F, Do LTK, Otoi T. 2022

[雑誌論文] Gene editing in porcine embryos using a combination of electroporation and transfection methods

Takebayashi Koki、Wittayarat Manita、Lin Qingyi、Hirata Maki、Yoshimura Naoaki、Torigoe Nanaka、Nagahara Megumi、Do Lanh Thi Kim、Tanihara Fuminori、Otoi Takeshige 2022

[雑誌論文] Effects of the timing of electroporation during in vitro maturation on triple gene editing in porcine embryos using CRISPR/Cas9 system

Namula Zhao、Wittayarat Manita、Do Lanh Thi Kim、Van Nguyen Thanh、Lin Qingyi、Takebayashi Koki、Hirata Maki、Tanihara Fuminori、Otoi Takeshige 2022

[雑誌論文] Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods

Le Quynh Anh、Wittayarat Manita、Namula Zhao、Lin Qingyi、Takebayashi Koki、Hirata Maki、Tanihara Fuminori、Do Lanh Thi Kim、Otoi Takeshige 2022

[学会発表] Comparison of chemically mediated CRISPR/Cas9 gene-editing systems using different transfection mechanisms on the mutation of porcine embryos.

Lin Q, Hirata M, Takebayashi K, Torigoe, N., Nagahara, M., Otoi T. 2022