ＲＮＡ成熟マシナリーの構造的基盤と機能進化 - MATCI 産学連携支援マッチング情報システム

研究課題番号	KAKENHI-PROJECT-23350081
研究種目	基盤研究(B)
研究分野	理工系化学複合化学生体関連化学
研究機関	愛媛大学
代表研究者	堀弘幸
研究分担者	鈴木勉
研究分担者	平田章
研究期間開始年月日	2011/4/1
研究期間終了年度	2013
研究ステータス	完了 (2013/4/1)
配分額（合計）	19,370,000 (直接経費 :14,900,000、間接経費 :4,470,000)
配分額（履歴）	2013年度：4,160,000 (直接経費 :3,200,000、間接経費 :960,000) 2012年度：4,290,000 (直接経費 :3,300,000、間接経費 :990,000) 2011年度：10,920,000 (直接経費 :8,400,000、間接経費 :2,520,000)
キーワード	核酸酵素タンパク質ＲＮＡ RNA RNA修飾 RNAメチル化 tRNA メチル化酵素 RNA分解制御ゲノム構造解析

[雑誌論文] Methylated nucleosides in tRNA and tRNA methyltransferase

H. Hori 2014

[雑誌論文] Methylated nucleosides in tRNA and tRNA methyltransferases

H. Hori 2014

[学会発表] Eukaryotic tRNA (m2G10) methyltransferase (Trm11-Trm112) recognizes the whole structure of tRNA

Shiho Ohmori, Yu Nishida, Kazuki Okada, Akira Hirata, and Hiroyuki Hori 2013

[学会発表] 葉酸依存性tRNA(m5U54)メチル化酵素TrmFOはどうやってtRNA中のU54を認識しているのか?

山上龍太, 山下光輝, 西増弘志, 冨川千恵, 越智杏奈, 岩下智香子, 平田章, 石谷隆一郎, 濡木理, 堀弘幸 2013

[学会発表] X-ray crystal structure of the fourth type of tRNA splicing endonuclease from an uncultivated archaeon Candidatus Micrarchaeum acidiphilum (ARMAN-2)

Akira Hirata, Kosuke Fujishima, Ryota Yamagami, Takuya Kawamura, Jillian F. Banfiled, Akio Kanai and Hiroyuki Hori 2013

[雑誌論文] The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process

A. Ochi, K. Makabe, R. Yamagami, A. Hirata, R. Sakaguchi, Y.M. Hou, K. Watanabe, O. Nureki, K. Kuwajima, and H. Hori 2013

[学会発表] Thermococcus kodakarensis 由来tRNAメチル化酵素(Trm-m22G10)の構造機能解析

西山聖示, 平田章, 田村俊浩, 堀弘幸 2013

[学会発表] 極小アーキア(古細菌)における4番目のタイプtRNAスプライシングエンドヌクレアーゼのX線結晶構造

平田章, 藤島皓介, 山上龍太, 河村卓哉, Jillina F. Banfield, 金井昭夫, 堀弘幸 2013

[学会発表] Does D20 formation by eubacterial dihydrouridine synthase require the other modified nucleotides for its modification in tRNA?

楠葉浩晃, 吉田剛士, 岩崎絵梨, 粟井貴子, 平田章, 冨川千恵, 山上龍太, 堀弘幸 2013

[学会発表] 真正細菌ジヒドロウリジン合成酵素はD20形成にtRNA上の他の修飾ヌクレオチドを必要とする

楠葉浩晃, 吉田剛士, 岩崎絵梨, 粟井貴子, 平田章, 冨川千恵, 山上龍太, 堀弘幸 2013

[学会発表] 超好熱性アーキアThermococcus kodakarensis 2´-5´RNAリガーゼの機能解析

平田章, 長野倫子, 石丸雄基, 金井保, 鈴木健夫, 佐藤朝子, 金井昭夫, 鈴木勉, 堀弘幸 2013

[学会発表] Yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明

大森志穂, 西田裕, 岡田和樹, 平田章, 堀弘幸 2013

[雑誌論文] The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process.

A. Ochi, K. Makabe, R. Yamagami, A. Hirata, R. Sakaguchi, Y.M. Hou, K. Watanabe, O. Nureki, K. Kuwajima, and H. Hori 2013

[学会発表] 超好熱性アーキアThermococcus kodakarensis由来tRNATrpの修飾ヌクレオシド分析

前田雄太, 平田章, 長野倫子, 土手智裕, 鈴木健夫, 金井保, 鈴木勉, 堀弘幸 2013

[学会発表] 極小アーキア(古細菌）における4番目のタイプtRNAスプライシングエンドヌクレアーゼのX線結晶構造

平田　章、藤島皓介、山上龍太、河村卓哉、Jillina F. Banfield、金井昭夫、堀　弘幸 2013

[学会発表] Novel tRNA modifications in the thermophilic-acidophilic archaeon, Thermoplasma acidophilum

Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Takuya Kawamura, Akihiko Yamagishi, Tsutomu Suzuki, and Hiroyuki Hori 2013

[学会発表] The synthesis mechanism of a tRNA modification in thermophilic bacteria

Takuya Kawamura, Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Nobukazu Nameki, Natsuhisa Oka, Hiroki Taniguchi, Kaori Ando, Satoshi Ohno, Akihiko Yamagishi, Tsutom Suzuki, Takashi Yokogawa, and Hiroyuki Hori 2013

[学会発表] m7G46およびΨ55を中心としたThermus thermophilustRNA修飾ネットワーク

冨川千恵, 石田一雄, 国林貴史, 金井保, 横川隆志, 堀弘幸 2013

[図書] 実験医学増刊号生命分子を統合するRNA-その秘められた役割と制御機構1-3 tRNA修飾と機能

堀弘幸 2013

[学会発表] The synthesis mechanism of a tRNA modification in thermophilic bacteria

Takuya Kawamura, Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Nobukazu Nameki, Natsuhisa Oka, Hiroki Taniguchi, Kaori Ando, Satoshi Ohno, Akihiko Yamagishi, Tsutom Suzuki, Takashi Yokogawa, and Hiroyuki Hori 2013

[雑誌論文] Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells

K. Watanabe, R. Miyagawa, C. Tomikawa, R. Mizuno, A. Takahashi, H. Hori, and K. Ijiri 2013

[学会発表] 固相化DNAプローブ法を用いた好熱菌tRNAの大量精製法の確立

Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori 2013

[学会発表] 熱ストレスによる開始tRNAMetの細胞内動態

渡邉和則, 宮川隆, 冨川千恵, 水野利恵, 高橋昭久, 大槻高史, 堀弘幸, 井尻憲一 2013

[学会発表] Thermoplasma acidophilum 由来tRNALeuにおいてArchaeosine が2カ所に形成されるメカニズムの解析

河村卓哉, 冨川千恵, 大平高之 , 井上仁, 行木信一, 岡夏央, 谷口浩輝, 安藤香織, 大野敏, 山岸明彦, 鈴木勉, 横川隆志, 堀弘幸 2013

[学会発表] Prediction of genes for tRNA modifications in the thermoacidophilic archaeon, Thermoplasma acidophilum

Ryo Anraku, Miyu Higashiguchi, Takuya Kawamura, and Hiroyuki Hori 2013

[図書] 「生命分子を統合するRNA-その秘められた役割と制御機構」1-3「tRNA修飾の構造と機能」実験医学増刊号

堀弘幸 2013

[学会発表] Alteration of solid-phase DNA probe method for large-scale tRNA purification

Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori 2013

[雑誌論文] Distinct tRNA modifications in the thermo-acidophilic archaeon, Thermoplasma acidophilum

C. Tomikawa, T. Ohira, Y. Inoue, T. Kawamura, A. Yamagishi, T. Suzuki, and H. Hori 2013

[学会発表] X-ray crystal structure of the fourth type of tRNA splicing endonuclease from an uncultivated archaeon Candidatus Micrarchaeum acidiphilum (ARMAN-2)

Akira Hirata, Kosuke Fujishima, Ryota Yamagami, Takuya Kawamura, Jillian F. Banfiled, Akio Kanai and Hiroyuki Hori 2013

[学会発表] Novel tRNA modifications in the thermophilic-acidophilic archaeon, Thermoplasma acidophilum

Chie Tomikawa, Takayuki Ohira, Yasushi Inoue, Takuya Kawamura, Akihiko Yamagishi, Tsutomu Suzuki, and Hiroyuki Hori 2013

[学会発表] Prediction of genes for tRNA modifications in the thermoacidophilic archaeon, Thermoplasma acidophilum

Ryo Anraku, Miyu Higashiguchi, Takuya Kawamura, and Hiroyuki Hori 2013

[学会発表] Alteration of solid-phase DNA probe method for large-scale tRNA purification

Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori 2013

[雑誌論文] Degradation of initiator tRNAMet by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells.

K. Watanabe, R. Miyagawa, C. Tomikawa, R. Mizuno, A. Takahashi, H. Hori, and K. Ijiri 2013

[学会発表] Improvement of a protein purification method for nucleic acid-free in the case of recombinant tRNA (N2, N2-guanine)-dimethyltransferase (Trm1) from Aquifex aeolicus

S. Nishiyama, A. Hirata, T. Awai, and H. Hori 2012

[学会発表] Structural insight into the evolution and broad substrate specificity of the fourth type of tRNA splicing endonuclease

A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfiled, A. Kanai, and H. Hori 2012

[学会発表] 高度好熱菌tRNA中のシュードウリジン55修飾は, 温度変化への適応因子として機能する

冨川千恵, 石田一雄, 国林貴史, 越智杏奈, 金井保, 平田章, 岩下知香子, 堀弘幸 2012

[学会発表] Functional analysis of yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)

Y. Nishida, K. Okada, A. Hirata, and H. Hori 2012

[学会発表] Transfer RNA recognition mechanism of Thermus thermophilus folate/FAD-dependent tRNA methyltransferase (TrmFO)

R. Yamagami, K. Yamashita, H. Nishimasu, C. Tomikawa, A. Ochi, C. Iwashita, A. Hirata, R. Ishitani, O. Nureki, and H. Hori 2012

[学会発表] Expression and purification of yeast tRNA (Cm32/Gm34) methyltransferase (Trm7)

K. Yoshii, A. Hirata, and H. Hori 2012

[雑誌論文] The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO)

R. Yamagami, K. Yamashita, H. Nishimasu, C. Tomikawa, A. Ochi, C. Iwashita, A. Hirata, R. Ishitani, O. Nureki, and H. Hori 2012

[雑誌論文] X-ray structure of the fourth type of archaeal tRNA splicing endonuclease : insight into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity

A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfield, A. Kanai, and H. Hori 2012

[学会発表] 極小アーキアにおけるtRNAスプライシングエンドヌクレアーゼの構造機能解析

平田章, 藤島皓介, 山上龍太, 河村卓哉, J. F. Banfield, 堀弘幸 2012

[学会発表] サーマス・サーモフィラス由来tRNAメチル化酵素TrmFOの基質認識機構

山上龍太, 山下光輝, 西増弘志, 冨川千恵, 越智杏奈, 岩下智香子, 平田章, 石谷隆一郎, 濡木理, 堀弘幸 2012

[学会発表] Structural and functional analyses of archaeal 2'-5' RNA ligase

T. Nagano, A. Hirata, Y. Ishimaru, T. Kanai, and H. Hori 2012

[学会発表] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明

西田裕, 岡田和樹, 平田章, 堀弘幸 2012

[学会発表] Negative control of Gm18, m5s2U54 and m1A58 modifications by pseudouridine55 modification in Thermus thermophilus tRNAs : the tRNA modification network in extreme thermophile

C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori 2012

[学会発表] Research for substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)

H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori 2012

[学会発表] Substrate transfer RNA recognition mechanism of tRNA (m5U54) methyltransferase (TrmFO) based on the biochemical and structural analysis.

R. Yamagami, K. Yamashita, H. Nishimasu, C. Iwashita, A. Hirata, O. Nureki, and H. Hori 2012

[学会発表] Adaptation of Thermus thermophilus to environmental temperature via tRNA modification enzymes-modified nucleotides network in tRNA

C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori 2012

[学会発表] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)

H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori 2012

[学会発表] tRNA modifications in thermoacidophilic archaeon

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori 2012

[学会発表] Transfer RNA modifications in thermophilic bacteria

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori 2012

[雑誌論文] The 2'-O-methylation status of a single guanosine controls transfer RNA-mediated Toll-like receptor 7 activation or inhibition

S. Jöckel, G. Nees, R. Sommer, Y. Zhao, D. Cherkasov, H. Hori, G. Ehm, M. Schnare, M. Nain, A. Kaufmann, and S. Bauer 2012

[学会発表] RNA修飾成熟マシナリーの構造と機能

堀弘幸 2012

[学会発表] Visiting Yellowstone National Park, the place of discovery of thermophiles

H. Hori 2012

[学会発表] tRNA modifications in Thermoplasma acidophilum

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori 2012

[雑誌論文] X-ray structure of the fourth type of archaeal tRNA splicing endonuclease: insight into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity.

A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfield, A. Kanai, and H. Hori 2012

[学会発表] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)

Hiroyuki Takuma, Masayuki Minoji, Natsumi Ushio, Chie Tomikawa, Akira Hirata, Anna Ochi, Hiroyuki Hori 2011

[学会発表] Structural and functional analyses of archaeal 2'-5' RNA ligase

T. Nagano, A. Hirata, Y. Ishimaru, T. Kanai, and H. Hori 2011

[学会発表] Substrate tRNA recognition mechanism of FAD/folate-dependet tRNA m^5U54 methyltransferase TrmFO

R.Yamagami, K.Yamashita, H.Nishimasu, C.Iwashita, A.Hirata, O.Nureki, H.Hori 2011

[学会発表] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の機能解析

西田裕, 岡田和樹, 平田章, 堀弘幸 2011

[学会発表] Regulation of tRNA modification network by pseudouridine 55 in Thermus thermophiles

C.Tomikawa, K.Ishida, T.Kunibayashi, A.Ochi, T.Kanai, A.Hirata, C.Iwashita, H.Hori 2011

[学会発表] サーマス・サーモフィラス由来葉酸依存性tRNA (m5U54)メチル化酵素の基質認識機構

山上龍太、山下光輝、西増弘志、岩下知香子、平田章、濡木理、堀弘幸 2011

[学会発表] Thermus thermophilus tRNA (m1A58) methyltransferase [Trm1]の基質認識メカニズムの解明

詫間浩之, 美濃地真之, 牛尾なつみ, 冨川千恵, 平田章, 堀弘幸 2011

[学会発表] Transfer RNA recognition mechanism of multi-site specific tRNA methyltransferase (Trm1)from Aquifex aeolicus

T.Awai, A.Ochi, Ihsanawati, A.Hirata, Y.Bessho, S.Yokoyama, H.Hori 2011

[学会発表] Transfer RNA recognition mechanism of multi-site specific tRNA methyltransferase (Trm1) from Aquifex aeolicus

T. Awai, A. Ochi, Ihsanawati, A. Hirata, Y. Bessho, S. Yokoyama, and H. Hori 2011

[学会発表] Elucidation of substrate tRNA recognition mechanism by tRNA (m1A58) methyltransferase[TrmI] from Thermus thermophiles

H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, C. Iwashita, A. Ochi, and H. Hori 2011

[雑誌論文] Cleavage of intron from the standard or non-standard position of the precursor tRNA by the splicing endonuclease of Aeropyrum pernix, a hyper-thermophilic Crenarchaeon, involves a novel RNA recognition site in the Crenarchaea specific loop

A. Hirata, T. Kitajima, and H. Hori 2011

[学会発表] Multisite specific tRNA methyltransferase Trm1 from a hyper-thermophilic eubacterium, Aquifex aeolicus

T. Awai, A. Ochi, Ihsanawati, T. Sengoku, S. Kimura, C. Tomikawa, T. Yokogawa, A. Hirata, Y. Bessho, T. Suzuki, S. Yokoyama and H. Hori 2011

[学会発表] 古細菌における2'-5' RNA ligaseの構造機能解析

長野倫子, 平田章, 石丸雄基, 金井保, 堀弘幸 2011

[雑誌論文] Cleavage of intron from the standard or non-standard position of the precursor tRNA by the splicing endonuclease of Aeropyrum pernix, a hyper-thermophilic Crenarchaeon, involves a novel RNA recognition site in the Crenarchaea specific loop

A.Hirata, T.Kitajima, H.Hori 2011

[学会発表] Thermus thermophilus tRNA (m1A58) methyltransferase[Trm1]の基質認識メカニズムの解明

詫間浩之、美濃地真之、牛尾なつみ、冨川千恵、平田章、堀弘幸 2011

[学会発表] アーキアにおける2'-5' RNA ligaseの構造機能解析

長野倫子, 石丸雄基, 金井保, 平田章, 堀弘幸 2011

[学会発表] Recognition and cleavage of intron in precursor tRNA by the splicing endonuclease from Aperopyrum pernix, an aerobic hyper-thermophilic Crenarchaeon, thorough a novel RNA recognition site in Crenarchaea specific loop

A. Hirata, T. Kitajima, and H. Hori 2011

[学会発表] Regulation of tRNA modification network by pseudouridine 55 in Thermus thermophiles

C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori 2011

[学会発表] アーキアにおける2'-5' RNA ligaseの構造機能解析

長野倫子、石丸雄基、金井保、平田章、堀弘幸 2011

[学会発表] The crystal structure analysis and establishment of activity mesurement system of Thermus thermophilus tRNA(Um34, Cm34) methyltransferase[YibK]

T.Matsugi, A.Hirata, H.Hori 2011

[学会発表] 超好熱性古細菌Thermococcus kodakaraensis由来tRNA(m2G10/m22G10) methyltransferase (TK0981)の酵素学的性質の決定

田村俊浩, 平田章, 堀弘幸 2011

[学会発表] アーキアRNAポリメラーゼのサブユニットDに見られる特異的ドメイン構造と機能の多様性と進化

平田章、堀弘幸、村上勝彦、Faith H.Lessner、Matthew Jenning、Eduardus, C.Duin、Daniel J.Lessner 2011

[学会発表] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の機能解析

西田裕, 岡田和樹, 平田章, 堀弘幸 2011

[学会発表] A conserved Lys residue in Crenarchaea specific loop determines a broad substratespecificity of Crenarchaeal RNA splicing endonuclease

A. Hirata, T. Kitajima, and H. Hori 2011

[学会発表] 古細菌における2' - 5' RNA ligaseの構造機能解析

長野倫子, 平田章, 石丸雄基, 金井保, 堀弘幸 2011

[雑誌論文] Substrate tRNA recognition mechanism of a multi-site-specific tRNA methyltransferase, Aquifex aeolicus Trm1, based on the X-ray crystal structure

T. Awai, A. Ochi, Ihsanawati, T. Sengoku, A. Hirata, Y. Bessho, S. Yokoyama, and H. Hori 2011

[学会発表] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)

Hiroyuki Takuma, Masayuki Minoji, Natsumi Ushio, Chie Tomikawa, Akira Hirata, Anna Ochi, and Hiroyuki Hori 2011

[学会発表] The crystal structure analysis and establishment of activity mesurement system of Thermus thermophilus tRNA (Um34, Cm34) methyltransferase [YibK]

T. Matsugi, A. Hirata, and H. Hori 2011

[学会発表] Substrate tRNA recognition mechanism of FAD/folate-dependet tRNA m5U54 methyltransferase TrmFO

R. Yamagami, K. Yamashita, H. Nishimasu, C. Iwashita, A. Hirata, O. Nureki, and H. Hori 2011

[学会発表] サーマス・サーモフィラス由来葉酸依存性tRNA(m5U54)メチル化酵素の基質認識機構

山上龍太, 山下光輝, 西増弘志, 岩下知香子, 平田章, 濡木理, 堀弘幸 2011

[学会発表] Elucidation of substrate tRNA recognition mechanism by tRNA (m^1A58)methyltransferase[TrmI]from Thermus thermophiles

H.Takuma, M.Minoji, N.Ushio, C.Tomikawa, A.Hirata, C.Iwashita, A.Ochi, H.Hori 2011

[学会発表] A conserved Lys residue in Crenarchaea specific loop determines a broad substratespecificity of Crenarchaeal RNA splicing endonuclease

A.Hirata, T.Kitajima, H.Hori 2011

[学会発表] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の機能解析

西田裕、岡田和樹、平田章、堀弘幸 2011

[学会発表] 酵母由来のtRNA (m^2G10) methyltransferase (Trm11-Trm112)の機能解析

芦田裕、岡田和樹、平田章、堀弘幸 2011

[雑誌論文] Pseudouridine at position 55 (Ψ55) in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus

K. Ishida, T. Kunibayashi, C. Tomikawa, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori 2011

[学会発表] RNA修飾酵素の触媒機構の多様性とそのネットワークの環境応答

堀弘幸 2011

[学会発表] Transfer RNA modifications in Thermoplasma acidophilum

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori 2011

[学会発表] Relationship between 5-methyluridine modification at position 54 in tRNA and tRNA modification network in Thermus thermophiles

R. Yamagami, N. Shigi, S.-I. Asai, K. Watanabe, and H. Hori 2011

[図書] Degradation of hypo-modified tRNA in Thermus thermophilus, an extreme-thermophilic eubacteria, Advanced in Genetic Research, Vol. 6 (K. V. Urbano eds)

C. Tomikawa and H. Hori 2011

[学会発表] Transfer RNA modification in Thermoplasma acidophilum

T.Kawamura, C.Tomikawa, T.Ohira, Y.Inoue, A.Yamagishi, T.Suzuki, H.Hori 2011

[学会発表] Substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)

H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori

[学会発表] Functional analysis of yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)

Y. Nishida, K. Okada, A. Hirata, and H. Hori

[学会発表] Eukaryotic tRNA (m2G10) methyltransferase (Trm11-Trm112) recognizes the whole structure of tRNA

Shiho Ohmori, Yu Nishida, Kazuki Okada, Akira Hirata, and Hiroyuki Hori

[学会発表] Expression and purification of yeast tRNA (Cm32/Gm34) methyltransferase (Trm7)

K. Yoshii, A. Hirata, and H. Hori

[学会発表] 極小アーキアにおけるtRNAスプライシングエンドヌクレアーゼの構造機能解析

平田章，藤島皓介，山上龍太，河村卓哉，J. F. Banfield，堀　弘幸

[学会発表] サーマス・サーモフィラス由来tRNA メチル化酵素TrmFOの基質認識機構

山上龍太，山下光輝，西増弘志，冨川千恵，越智杏奈，岩下智香子，平田　章，石谷隆一郎，濡木　理，堀　弘幸

[学会発表] Structural insight into the evolution and broad substrate specificity of the fourth type of tRNA splicing endonuclease

A. Hirata, K. Fujishima, R. Yamagami, T. Kawamura, J. F. Banfiled, A. Kanai, and H. Hori

[学会発表] Research for substrate tRNA recognition mechanism of eubacterial tRNA (m1A58) methyltransferase (TrmI)

H. Takuma, M. Minoji, N. Ushio, C. Tomikawa, A. Hirata, A. Ochi and H. Hori

[学会発表] Adaptation of Thermus thermophilus to environmental temperature via tRNA modification enzymes-modified nucleotides network in tRNA

C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori

[学会発表] Structural and functional analyses of archaeal 2’-5’ RNA ligase

T. Nagano, A. Hirata, Y. Ishimaru, T. Kanai, and H. Hori

[学会発表] Thermococcus kodakarensis 由来tRNA メチル化酵素(Trm- m22G10)の構造機能解析

西山聖示、平田　章、田村俊浩、堀　弘幸

[学会発表] Does D20 formation by eubacterial dihydrouridine synthase require the other modified nucleotides for its modification in tRNA?

楠葉浩晃、吉田剛士、岩崎絵梨、粟井貴子、平田　章、冨川千恵、山上龍太、堀　弘幸

[学会発表] 真正細菌ジヒドロウリジン合成酵素はD20形成にtRNA上の他の修飾ヌクレオチドを必要とする

楠葉浩晃、吉田剛士、岩崎絵梨、粟井貴子、平田　章、冨川千恵、

[学会発表] 酵母由来のtRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明

西田　裕、岡田和樹、平田　章、堀　弘幸

[学会発表] Substrate transfer RNA recognition mechanism of tRNA (m5U54) methyltransferase (TrmFO) based on the biochemical and structural analysis

R. Yamagami, K. Yamashita, H. Nishimasu, C. Iwashita, A. Hirata, O. Nureki, and H. Hori

[学会発表] Transfer RNA recognition mechanism of Thermus thermophilus folate/FAD-dependent tRNA methyltransferase (TrmFO)

R. Yamagami, K. Yamashita, H. Nishimasu, C. Tomikawa, A. Ochi, C. Iwashita, A. Hirata, R. Ishitani, O. Nureki, and H. Hori

[学会発表] Improvement of a protein purification method for nucleic acid-free in the case of recombinant tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) from Aquifex aeolicus

S. Nishiyama, A. Hirata, T. Awai, and H. Hori

[学会発表] 超好熱性アーキア Thermococcus kodakarensis 由来 tRNATrp の

前田雄太、平田　章、長野倫子、土手智裕、鈴木健夫、金井　保、鈴木　勉、堀　弘幸

[学会発表] 超好熱性アーキアThermococcus kodakarensis 2', 5'- RNA リガーゼの機能解析

平田　章、長野倫子、石丸雄基、金井　保、鈴木健夫、佐藤朝子、金井昭夫、鈴木　勉、堀　弘幸

[学会発表] Negative control of Gm18, m5s2U54 and m1A58 modifications by pseudouridine55 modification in Thermus thermophilus tRNAs: the tRNA modification network in extreme thermophile

C. Tomikawa, K. Ishida, T. Kunibayashi, A. Ochi, T. Kanai, A. Hirata, C. Iwashita, and H. Hori

[学会発表] Yeast tRNA (m2G10) methyltransferase (Trm11-Trm112)の基質認識機構の解明

大森志穂、西田　裕、岡田和樹、平田　章、堀　弘幸

[学会発表] 高度好熱菌tRNA中のシュードウリジン55修飾は、温度変化への適応因子として機能する

冨川千恵，石田一雄，国林貴史，越智杏奈，金井　保，平田　章，岩下知香子，堀　弘幸

[学会発表] 葉酸依存性tRNA(m5U54)メチル化酵素TrmFOはどうやってtRNA中のU54を認識しているのか?

山上龍太, 山下光輝, 西増弘志, 冨川千恵, 越智杏奈, 岩下智香子, 平田　章, 石谷隆一郎, 濡木　理, 堀　弘幸

[学会発表] 熱ストレスによる開始tRNAMetの細胞内動態

渡邉和則、宮川　隆、冨川千恵、水野利恵、高橋昭久、大槻高史、堀弘幸、井尻憲一

[学会発表] m7G46およびΨ55を中心としたThermus thermophilus tRNA修飾ネットワーク

冨川千恵、石田一雄、国林貴史、金井　保、横川隆志、堀　弘幸

[学会発表] RNAメチル化酵素の構造と反応機構の多様性

堀　弘幸

[学会発表] RNAメチル化酵素の構造と反応機構の多様性：トポロジカル・ノット型RNAメチル化酵素発見からの８年間

堀　弘幸

[学会発表] tRNA modifications in thermoacidophilic archaeon

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori

[学会発表] 固相化DNAプローブ法を用いた好熱菌tRNAの大量精製法の確立

Ai Kazayama, Ryota Yamagami, Takashi Yokogawa, and Hiroyuki Hori

[学会発表] tRNA modifications in Thermoplasma acidophilum

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori

[学会発表] Transfer RNA modifications in thermophilic bacteria

T. Kawamura, C. Tomikawa, T. Ohira, Y. Inoue, A. Yamagishi, T. Suzuki, and H. Hori

[学会発表] Thermoplasma acidophilum由来tRNALeuにおいてArchaeosineが2カ所に形成されるメカニズムの解析

河村卓哉, 冨川千恵, 大平高之, 井上　仁, 行木信一, 岡　夏央, 谷口浩輝, 安藤香織, 大野　敏,山岸明彦, 鈴木　勉, 横川隆志, 堀　弘幸

研究成果