トップ›研究者を探す›DNA内メチルシトシン塩基の光酸化反応機構の解明と新規DNAメチル化検出系の探索

DNA内メチルシトシン塩基の光酸化反応機構の解明と新規DNAメチル化検出系の探索

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-21710228
研究種目 若手研究(B)
研究分野 総合・新領域系
複合新領域
生体分子科学
生物分子科学
研究機関 京都大学
代表研究者 山田 久嗣
研究期間 開始年月日 2009/4/1
研究期間 終了年度 2010
研究ステータス 完了 (2010/4/1)
配分額（合計） 4,420,000 (直接経費 :3,400,000、間接経費 :1,020,000)
配分額（履歴） 2010年度：1,430,000 (直接経費 :1,100,000、間接経費 :330,000)
2009年度：2,990,000 (直接経費 :2,300,000、間接経費 :690,000)
キーワード 機能性核酸
光増感反応
DNA内電荷移動反応
5-メチルシトシン
同位体効果
DNA内電荷移動
DNAメチル化
修飾シトシン塩基
重水素同位体効果

研究成果

[雑誌論文] Preparation of Functionalized Oligodeoxynucleotides and Photochemical One-Electron Oxidation of 5-Methylcytosine in DNA

Tanabe, K., Yamada, H., Nishimoto S. 2011

[雑誌論文] Anthraquinone-Sensitized Photooxidation of 5-Methylcytosine in DNA Leading to Piperidine-Induced Efficient StrandCleavage

Yamada, H., Kitauchi, Y., Tanabe, K., Ito, T., Nishimoto, S. 2011

[雑誌論文] Photoinduced Electron Injection into DNA by N-Cyclopropyl-1-aminonaphthalene

Ito, T.; Uchida, T.; Tanabe, K.; Yamada, H.; Nishimoto, S. 2011

[雑誌論文] Anthraquinone-Sensitized Photooxidation of 5-Methylcytosine in DNA Leading to Piperidine-Induced Efficient Strand Cleavage

Yamada, H.; Kitauchi, Y.; Tanabe, K.; Ito, T.; Nishimoto, S. 2011

[学会発表] メチル化DNAの光増感酸化反応に及ぼす重水素同位体効果

山田久嗣、北内佑哉、田邉一仁、伊藤健雄、西本清一 2010

[学会発表] 光化学反応を利用したDNAメチル化検出法の開発

山田久嗣 2010

[学会発表] Efficient Hole Trapping at C(5)-Substituted Cytosine in the Photosensitizer-Injected Positive Charge Transfer through DNA Duplex

Kurata, M., Yamada, H., Tanabe, K., Ito, T., Nishimoto, S. 2010

[学会発表] Efficient Hole Trapping at C(5)-Substituted Cytosine in the Photosensitizer-Injected Positive Charge Transfer through DNA Duplex

Kurata, M.; Yamada, H.; Tanabe, K.; Ito, T.; Nishimoto, S. 2010

[学会発表] One-Electron Oxidation at 5-Methylcytosine Induced by the Photosensitizer-Injected Positive Charge Transfer through DNA

Kitauchi, Y.; Yamada.H.; Tanabe, K.; Ito, T.; Nishimoto, S. 2010

[学会発表] Efficient one-electron photooxidation and site-selective strand cleavage at 5-methylcytosine in DNA by oligonucleotide bearing 9,10-anthraquinone sensitizer

Kitauchi, Y., Yamada, H., Tanabe, K., Ito, T., Nishimoto, S. 2010

[学会発表] Selective trapping at cytosine derivatives of photosensitizerinjected and migrated hole in DNA

Kurata, M.; Yamada, H.; Tanabe, K.; Ito, T.; Nishimoto, S. 2010

[学会発表] Selective trapping at cytosine derivatives of photosensitizer-injected and migrated hole in DNA

Kurata, M., Yamada, H., Tanabe, K., Ito, T., Nishimoto, S. 2010

[学会発表] Efficient one-electron photooxidation and site-selective strand cleavage at 5-methylcytosine in DNA by oligonucleotide bearing 9, 10-anthraquinone sensitizer

Kitauchi, Y.; Yamada, H.; Tanabe, K.; Ito, T.; Nishimoto, S. 2010

[学会発表] メチル化DNAの光増感酸化反応に及ぼす重水素同位体効果

山田久嗣、北内佑哉、田邉一仁、伊藤健雄、西本清一 2010

[学会発表] Photoelectrochemical Identification of 5-Methylcytosine Modification in DNA : Combination of Photosensitization and Enzymatic Cleavage

Tanabe, K., Yamada, H., Ito, T., Nishimoto,S. 2009

[学会発表] Photochemical Approach to Discrimination of Methylated Cytosine in DNA : pH Effect on theNaphthoquinone-Sensitized One-Electron Photooxidation of 5-Methylcytosine

Yamada, H., Kitauchi, Y., Tanabe, K., Ito, T., Nishimoto, S. 2009

[雑誌論文] Efficient Photooxidative Strand Cleavage at 5-Methylcytosine in DNA by Sensitization with 9,10-Anthraquinone-Tethered Oligonucleotides

Yamada, H, ; Kitauchi, Y.; Tanabe, K.; Ito, T.; Nishimoto, S. 2009

[学会発表] Efficient Photooxidative Strand Cleavage at 5-Methylcytosine in DNA by Sensitization with 9,10-Anthraquinone-Tethered Oligonucleotides

山田久嗣、北内佑哉、田邉一仁、伊藤健雄、西本清一 2009

[学会発表] アントラキノン光増感剤を導入したオリゴDNA : 5-メチルシトシン塩基部位での選択的DNA切断

北内佑哉、山田久嗣、田邉一仁、伊藤健雄、西本清一 2009

[学会発表] Efficient Photooxidative Strand Cleavage at 5-Methylcytosine in DNA by Sensitization with9,10-Anthraquinone-Tethered Oligonucleotides

Yamada, H., Kitauchi, Y., Tanabe, K., Ito, T., Nishimoto, S. 2009

[雑誌論文] Photo-Induced Electron Transfer Reaction in Diaminostilbene-Tethered DNA Duplexes

Ito, T., Hayashi, A., Uchida, T., Tanabe, K., Yamada, H., Nishimoto, S. 2009

[学会発表] C(5)位に置換基を導入した修飾シトシン含有DNAオリゴマーの合成と四重鎖形成特性

藏田真之、山田久嗣、田邉一仁、伊藤健雄、山田久嗣、西本清一 2009

[雑誌論文] Efficient Photooxidative Strand Cleavage at 5-Methylcytosine in DNA by Sensitization with 9,10-Anthraquinone-Tethered Oligonucleotides

Yamada, H., Kitauchi, Y., Tanabe, K., Ito, T., Nishimoto, S. 2009

[雑誌論文] Photoelectrochemical Identification of 5-Methylcytosine Modification in DNA : Combination of Photosensitization and Enzymatic Cleavage

Tanabe, K., Yamada, H., Ito, T.; Nishimoto, S. 2009

[雑誌論文] Photoelectrochemical Identification of 5-Methylcytosine Modification in DNA : Combination of Photosensitization and Enzymatic Cleavage

Tanabe, K.; Yamada, H.; Ito, T.; Nishimoto, S. 2009

[雑誌論文] Preparation of Functionalized Oligodeoxynucleotides and Photochemical One-Electron Oxidation of 5-Methylcytosine in DNA

Tanabe, K.; Yamada, H.; Nishimoto S.