触媒的タンパク質機能欠失法の開拓

トップ›研究者を探す›触媒的タンパク質機能欠失法の開拓

代表研究者	大高章
研究課題番号	KAKENHI-PROJECT-21390031
研究種目	基盤研究(B)
研究分野	生物系医歯薬学薬学創薬化学
研究機関	徳島大学
研究分担者	重永章
研究期間開始年月日	2009/4/1
研究期間終了年度	2011
研究ステータス	完了 (2011/4/1)
配分額（合計）	18,460,000 (直接経費 :14,200,000、間接経費 :4,260,000)
配分額（履歴）	2011年度：5,070,000 (直接経費 :3,900,000、間接経費 :1,170,000) 2010年度：5,980,000 (直接経費 :4,600,000、間接経費 :1,380,000) 2009年度：7,410,000 (直接経費 :5,700,000、間接経費 :1,710,000)
キーワード	ペプチドペプチドミメティック外部刺激応答型アミノ酸ペプチド結合切断標的タンパク質ホスファターゼ抵抗性リン酸化アミノ酸タンパク質ペプチド合成チオエステルタンパク濃縮刺激応答型アミノ酸ペプチド等価体デグロン

研究成果

[雑誌論文] Caged ceramide which releases parent ceramide after UV-induced amide bond cleavage followed by intramolecular O-N acyl transfer

Shigenaga, A.; Hirakawa, H.; Yamamoto, J.; Ogura, K.; Denda, M.; Yamaguchi, K.; Otaka, A 2012

[雑誌論文] N-Sulfanyl-ethylanilide peptide : a peptide thioester equivalent which can directly participate in native chemical ligation

Sato, K.; Tsuda, S.; Sakamoto, K.; Shigenaga, A.; Otaka, A 2012

[図書] 刺激応答型アミノ酸の開発と生命科学分野への応用

重永章、山本純、大高章 2012

[学会発表] N-Sulfanylethylanilides as a Versatile Chemical Device for the Protein Chemistry

Otaka, A 2012

[雑誌論文] Double-kinetically controlled proline ligation

Ding, H.; Sato, K.; Morishita, K.; Shigenaga, A.; Otaka, A 2012

[雑誌論文] Caged ceramide which releases parent ceramide after UV-induced amide bond cleavage followed by intramolecular O-N acyl transfer

Shigenaga, A. 2012

[図書] 遺伝子医学MOOK21号最新ペプチド合成技術とその創薬研究への応用「刺激応答型アミノ酸の開発と生命科学分野への応用」

重永章 2012

[雑誌論文] Fmoc-based solid phase synthesis of adenylylated peptides using diester-type adenylylated amino acid derivatives

Ogura, K.; Shigenaga, A.; Ebisuno, K.; Hirakawa, H.; Otaka, A 2012

[雑誌論文] N-Sulfanylethyl-anilide derivative as a peptide thioester equivalent

Sato, K.; Tsuda, S.; Tsuji, K.; Sakamoto, K.; Shigenaga, A.; Otaka, A 2011

[雑誌論文] Development of amide bond cleavage device with application to chemical biology use

Otaka, A.; Shigenaga, A 2011

[雑誌論文] Enantioselective synthesis of stimulus-responsive amino acid via pyrrolidinyl tetrazole catalyzed asymmetricα-amination of aldehyde

Shigenaga, A.; Yamamoto, J.; Nishioka, N.; Denda, M.; Otaka, A 2011

[雑誌論文] Synthesis of nonhydrolyzable AMPylated amino acid analogues for uncovering the physiological role of AMPylation

Ogura, K.; Hirakawa, H.; Shigenaga, A.; Otaka, A 2011

[雑誌論文] Development of UV-responsive catch-and-release system of a cysteine protease model peptide

Shigenaga, A.; Morishita, K.; Yamaguchi, K.; Ding, H.; Ebisuno, K.; Sato, K.; Yamamoto, J.; Akaji, K.; Otaka, A 2011

[雑誌論文] Design and synthesis of caged ceramide : UV-responsive ceramide releasing system based on UV-induced amide bond cleavage followed by O-N acyl transfer

Shigenaga, A.; Hirakawa, H.; Yamamoto, J.; Ogura, K.; Denda, M.; Yamaguchi, K.; Tsuji, D.; Itoh, K.; Otaka, A 2011

[雑誌論文] Synthesis of CXCL14 and its derivatives utilizing C to N or N to C directive sequential NCL protocol

Tsuji, K.; Sumikawa, Y.; Tanegashima, K.; Shigenaga, A.; Hara, T.; Otaka, A 2011

[雑誌論文] N-Sulfanylethylanilide peptide as a crypto-thioester peptide

Sato, K.; Shigenaga, A.; Tsuji, K.; Tsuda, S.; Sumikawa, Y.; Sakamoto, K.; Otaka, A 2011

[雑誌論文] Synthesis and biological evaluation of CXCL14 and its derivatives

Tsuji, K.; Sumikawa, Y.; Tanegashima, K.; Shigenaga, A.; Hara, T.; Otaka, A 2011

[雑誌論文] Application of N-C-or C-N-directed sequential native chemical ligation to the preparation of CXCL14 analogs and their biological evaluation

Tsuji, K.; Shigenaga, A.; Sumikawa, Y.; Tanegashima, K.; Sato, K.; Aihara, K.; Hara, T.; Otaka, A 2011

[雑誌論文] Development of recapturable cleavable linker for efficient enrichment and specific labeling of target proteins

Yamamoto, J.; Maeda, N.; Denda, M.; Shigenaga, A.; Otaka, A 2011

[雑誌論文] Native chemical ligation using N-peptidyl anilide as crypto-thioester

Sato, K.; Tsuda, S.; Maeda, N.; Denda, M.; Shigenaga, A.; Otaka, A 2011

[雑誌論文] Dual kinetically-controlled native chemical ligation using a combi-nation of sulfanylproline and sulfanyl-ethylanilide peptide

Ding, H.; Shigenaga, A.; Sato, K.; Morishita, K.; Otaka, A 2011

[雑誌論文] Development of stimulus responsive thiol releasing system for controlling activity of cysteine protease

Morishita, K.; Yamaguchi, K.; Ding, H.; Shigenaga, A.; Akaji, K.; Otaka, A 2011

[学会発表] Development of nucleocytoplasmic shuttle peptide using stimulus-responsive processing device

大高章 2011

[学会発表] Caged ceramide which releases parent ceramide after UV-induced amide bond cleavage followed by intramolecular O-N acyl transfer

重永章 2011

[雑誌論文] Enantioselective synthesis of stimulus-responsive amino acid via pyrrolidinyl tetrazole catalyzed asymmetric α-amination of aldehyde

Shigenaga, A. 2011

[学会発表] プロリン型触媒を用いた刺激応答型アミノ酸の不斉合成

重永章 2011

[学会発表] Development of amide bond cleavage device with application to chemical biology use

大高章 2010

[学会発表] Development of peptide bond cleavage device and its application for chemical biology use

大高章 2010

[雑誌論文] Development of thiol-responsive amide bond cleavage device and its application for peptide nucleic acid-based DNA releasing system

Shigenaga, A.; Yamamoto, J.; Hirakawa, H.; Ogura, K.; Maeda, N.; Morishita, K.; Otaka, A 2010

[雑誌論文] Development of amide bond cleavage device with application to chemical biology use

Otaka, A. 2010

[雑誌論文] Sequential native chemical ligation utilizing peptide thioacids derived from newly developed Fmoc-based synthetic method

Shigenaga, A.; Sumikawa, Y.; Tsuda, S.; Sato, K.; Otaka, A 2010

[雑誌論文] Proline cis/ trans-Isomerase Pin1 Regulates Peroxisome Proliferator-activated Receptor gamma Activity through the Direct Binding to the Activation Function-1 Domain

Fujimoto, Y.; Shiraki, T.; Horiuchi, Y.; Waku, T.; Shigenaga, A.; Otaka, A.; Ikura, T.; Igarashi, K.; Aimoto, S.; Tate, S.; Morikawa, K 2010

[図書] 第10章最近のペプチド・タンパク質の化学合成について

大高章、重永章 2010

[雑誌論文] Enantioselective synthesis of stimulus-responsive amino acid via asymmetricα-amination of aldehyde

Shigenaga, A.; Yamamoto, J.; Nishioka, N.; Otaka, A 2010

[雑誌論文] A structure-activity relationship study elucidating the mechanism of sequence-specific collagen recognition by the chaperone HSP47

Nishikawa, Y.; Takahara, Y.; Asada, S.; Shigenaga, A.; Otaka, A.; Kitagawa, K.; Koide, T 2010

[学会発表] Development of amide bond cleavage device with application to chemical biology use

Otaka, A.; Shigenaga, A 2010

[図書] 機能性タンパク質・ペプチドの生体利用(第10章最近のペプチド・タンパク質の化学合成について)

大高章 2010

[雑誌論文] ペプチドチオエステル合成法の最近の進展

Shigenaga, A.; Sato, K.; Otaka, A 2010

[雑誌論文] Synthesis of cyclic peptides via on resin-intramolecular native chemical ligation followed by reductive release from resin

Shigenaga, A. 2010

[学会発表] Enantioselective synthesis of stimulus-responsive amino acid via pyrrolidinyl tetrazole catalyzed asymmetric α-amination of aldehyde

重永章 2010

[学会発表] 刺激応答型ペプチド結合切断反応を基盤としたペプチド機能制御

重永章 2010

[雑誌論文] Development and photo-responsive peptide bond cleavage reaction of two-photon near-infrared excitation responsive peptide

Shigenaga, A. 2010

[学会発表] Development of stimulus-responsive amino acid with peptide bond-cleavage ability and its application for controlling peptidyl function

重永章 2010

[雑誌論文] Enantioselective synthesis of stimulus-responsive amino acid via asymmetric α-amination of aldehyde

Shigenaga, A. 2010

[雑誌論文] Development of thiol-responsive amide bond cleavage device and its application for DNA releasing system based on a peptide nucleic acid

Shigenaga, A. 2010

[雑誌論文] Sequential native chemical ligation utilizing peptide thioacids derived from newly developed Fmoc-based synthetic method

Shigenaga, A. 2010

[雑誌論文] ペプチドチオエステル合成法の最近の進展

重永章 2010

[雑誌論文] 十字路:セーフティーキャッチリンカー

重永章 2010

[雑誌論文] N-> S Acyl-Transfer-Mediated Synthesis of Peptide Thioesters Using Anilide Derivatives

Tsuda, S.; Shigenaga, A.; Bando, K.; Otaka, A 2009

[雑誌論文] Structural and functional analyses of minimal phosphopeptides targeting the polo-box domain of polo-like kinase 1

Yun, S. M.; Moulaei, T.; Lim, D.; Bang, J. K.; Park, J. E.; Shenoy, S. R.; Liu, F.; Kang, Y. H.; Liao, C. Z.; Soung, N. K.; Lee, S.; Yoon, D. Y.; Lim, Y.; Lee, D. H.; Otaka, A.; Appella, E.; McMahon, J. B.; Nicklaus, M. C.; Burke, T. R.; Yaffe, M. B.; Wlodawer, A.; Lee, K. S. 2009

[雑誌論文] Synthesis of Amide-Type Fluoroalkene Dipeptide Isosteres by an Intramolecular Redox Reaction

Yamaki, Y.; Shigenaga, A.; Li, J. L.; Shimohigashi, Y.; Otaka, A 2009

[雑誌論文] Synthesis of Fluoroalkene Dipeptide Isosteres by an Intramolecular Redox Reaction Utilizing N-Heteorocyclic Carbenes(NHCs)

Yamaki, Y.; Shigenaga, A.; Tomita, K.; Narumi, T.; Fujii, N.; Otaka, A 2009

[雑誌論文] FRET-based assay of the processing reaction kinetics of stimulus-responsive peptides : influence of amino acid sequence on reaction kinetics

Shigenaga, A.; Yamamoto, J.; Hirakawa, H.; Yamaguchi, K.; Otaka, A 2009

[学会発表] ペプチド・タンパク質機能制御法について考える

重永章 2009

[学会発表] N-置換アニリン型補助基を用いたペプチドチオエステル合成法の開発

重永章 2009

[学会発表] Synthesis of cyclic peptides via on resin-intramolecular native chemical ligation followed by reductive release from resin

重永章 2009

[学会発表] 刺激応答型アミノ酸の開発とペプチド機能制御への展開

重永章 2009

[学会発表] "化学"でペプチド・タンパク質を操る

重永章 2009