トップ研究者を探す尿路病原性大腸菌が産生するUSPの作用解析と粘膜ワクチンの開発

尿路病原性大腸菌が産生するUSPの作用解析と粘膜ワクチンの開発

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-20590436
研究種目 基盤研究(C)
研究分野 生物系
医歯薬学
基礎医学
細菌学(含真菌学)
研究機関 帯広畜産大学
代表研究者 倉園 久生
研究分担者 山本 新吾
研究分担者 山崎 栄樹
研究期間 開始年月日 2008/4/1
研究期間 終了年度 2010
研究ステータス 完了 (2010/4/1)
配分額(合計) 4,680,000 (直接経費 :3,600,000、間接経費 :1,080,000)
配分額(履歴) 2010年度:910,000 (直接経費 :700,000、間接経費 :210,000)
2009年度:1,820,000 (直接経費 :1,400,000、間接経費 :420,000)
2008年度:1,950,000 (直接経費 :1,500,000、間接経費 :450,000)
キーワード 病原性
粘膜ワクチン
細菌
尿路感染症
蛋白質
免疫学
粘膜
感染症

研究成果

[雑誌論文] Hybrid & EI Tor variant biotypes of Vibrio cholerae O1 in Thailand

Na-Ubol, M, Srimanote P, Chongsa-nguan M, Indrawattana N, Sookrung N, Tapchaisri P, Yamazaki S, Bodhidatta L, Eampokalap B, Kurazono H, Hayashi H, Nair GB, Takeda Y, Chaicumpa W 2011

[雑誌論文] Helicobacter pylori VacA reduces cellular expression of STAT3 and pro-survival Bcl-2 family proteins, Bcl-2 and Bcl-X_L, leading to apoptosis in gastric epithelial cells

Matsumoto A, Isomoto H, Nakayama M, Hisatsune J, Nishi Y, Nakashima Y, Matsushima K, Kurazono H, Nakao K, Hirayama T, Kohno S 2011

[雑誌論文] Hybrid & El Tor variant biotypes of Vibrio cholerae Ol in Thailand

Na-Ubol M, Kurazono H, Chaicumpa W 2011

[雑誌論文] Helicobacter pylori VacA reduces cellular expression of STAT3 and pro-survival Bcl-2 family proteins, Bcl-2 and Bcl-X_L, leading to apoptosis in gastric epithelial cells.

Matsumoto A, Kurazono H, Kohno S 2011

[雑誌論文] A V_HH that neutralizes the zinc-metalloproteinase activity of botulinum neurotoxin type A.

Thanongsaksrikul J, Kurazono H, Chaicumpa W 2010

[学会発表] 尿路病原性大腸菌の産生するUSPに対する特異的同定法の開発

山崎栄樹、高橋彰、山本新吾、星英之、牧野壮一、倉園久生 2010

[学会発表] Analysis of Pthogenicity island of Uropathogenic Escherichia coli which encodes the USP.

倉園久生倉園久生 2010

[学会発表] Analysis of Pthogenicity island of Uropathogenic Escherichia coli which encodes the USP

Kurazono H 2010

[学会発表] 尿路病原性大腸菌の産生するUSPに対する特異的同定法の開発

山崎栄樹, 高橋彰, 山本新吾, 星英之, 牧野壮一, 倉園久生 2010

[雑誌論文] A V_HH that neutralizes the zinc-metalloproteinase activity of botulinum neurotoxin type A.

Thanongsaksrikul J, Srimanote P, Maneewatch S, Choowongkomom K, Tapchaisri P, Makino S-I, Kurazono H, Chaicumpa W. 2010

[雑誌論文] Helicobacter pylori CagA Inhibits Endocytosis of Cytotoxin VacA in Host Cells

Akada KJ, Aoki H, Torigoe Y, Kitagawa T, Kurazono H, Hoshida H, Nishikawa J, Terai S, Matsuzaki M, Hirayama T, Nakazawa T, Akada R, Nakamura K 2010

[雑誌論文] A V_HH that neutralizes the zinc-metalloproteinase activity of botulinum neurotoxin type A

Thanongsaksrikul J, Srimanote P, Maneewatch S, Choowongkomon K, Tapchaisri P, Makino S-I, Kurazono H, Chaicumpa W 2010

[雑誌論文] Helicobacter pylori CagA Inhibits Endocytosis of Cytotoxin VacA in Host Cells.

Akada KJ, Kurazono H, Nakamura K 2010

[学会発表] Education for Sustainable Development (ESD) on Infectious Diseases and Global Environmental Issues

Smith M, Kurazono H 2010

[学会発表] Diversity in the expression of Salmonella enterotoxin that has a cytotoxic effect on cultured cells by various Salmonella species.

Yamasaki E, Nakano M, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, Nair G B, Makino S-I, Hirayama T, Kurazono H 2009

[雑誌論文] Genetic profiles of fluoroquinolone-resistant Escherichia coli isolated from cystitis : phylogeny, virulence factors, PAIusp-subtypes, and mutation patterns.

Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima, H, Kurazono H, Yamamoto S 2009

[雑誌論文] Genetic profiles of fluoroquinolone-resistant Escherichia coli isolated from cystitis : phylogeny, virulence factors, PAlusp-subtypes, and mutation patterns.

Takahashi A, Kurazono H, Yamamoto S 2009

[学会発表] The phylogenetic and virulence profiles of quinolone-resistant Escherichia coli isolated from cystitis are different from quinolone-susceptible counterparts.

Yamamoto S, Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima H, Kurazono H 2009

[学会発表] The phylogenetic and virulence profiles of quinolone-resistant Escherichia coli isolated from cystitis are different from quinolone-susceptible counterparts.

Yamamoto S, Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima H, Kurazono H 2009

[学会発表] Diversity in the expression of Salmonella enterotoxin that has a cytotoxic effect on cultured cells by various Salmonella species.

Yamasaki E, Nakano M, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, Nair G B, Makino S-I, Hirayama T, Kurazono H 2009

[雑誌論文] Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin.

Kulkeaw K, Sakolvaree Y, Srimanote P, Tongtawe P, Maneewatch S, Sookrung N, Tungtrongchitr A, Tapchaisri P, Kurazono H, Chaicumpa W. 2009

[雑誌論文] Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin

Kulkeaw K, SaKolvaree Y, Srimanote P, Tongtawe P, Maneewatch S, Sookrung N, Tungtrongchitr A, Tapchaisri P, Kurazono H, Chaicumpa W 2009

[雑誌論文] Helicobacter pylori VacA-induced inhibition of GSK3 through the PI3K/Akt signaling pathway

Nakayama M, Hisatsune J, Yamasaki E, Isomoto H, Kurazono H, Hatakeyama M, Azuma T, Yamaoka Y, Yahiro K. Moss J, Hirayama T. 2009

[雑誌論文] Genetic profiles of fluoroquinolone-resistant Escherichia colt isolated from cystitis : phylogeny, virulence factors, PAlusp-subtypes, and mutation patterns

Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima, H, Kurazono H. Yamamoto S 2009

[雑誌論文] Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin.

Kulkeaw K, Kurazono H, Chaicumpa W 2009

[学会発表] Salmonella enterotoxin (Stn)精製法の確立及び細胞毒性解析

山崎栄樹、星英之、牧野壮一、平山壽哉、倉園久生 2009

[学会発表] Helicobacter pylori CagA inhibits VacA endocytosis during infection

赤田純子、倉園久生、平山壽哉、中澤晶子、中村和行 2009

[雑誌論文] Helicobacter pylori VacA-induced inhibition of GSK3 through the PI3K/Akt signaling pathway.

Nakayama M, Yamasaki E, Kurazono H, Hirayama T 2009

[学会発表] H. pylori VacAの細胞毒性に及ぼすSphingomyelinaseの影響

久恒順三、小田真隆、櫻井純、由崎栄樹、倉園久生、平山壽哉 2009

[学会発表] Diversity in the expression of Salmonella enterotoxin that has a cytotoxic effect on cultured cells by various Salmonella species.

Yamasaki E, Nakano M, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, Nair G B, Makino S-I, Hirayama T, Kurazono H 2009

[学会発表] Proteomic analysis of growth promoting factor in Shigella flexneri

川瀬摂、門田修子、倉園久生、牧野壮一、川本恵子 2009

[学会発表] Biological activities of Salmonella enterotoxin

Yamasaki E, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, GB Nair, Makino S-I, Hirayama T, Kurazono H 2008

[学会発表] Helicobacter pylori VacA-induced IL-8 production in U937 cells is mediated by an increase in intracellular Ca2+, followed by activation of ATF-2, CREB and NF-kB

Toshiya Hirayama, Jyunzo Hisatsune, Eiki Yamasaki, Hisao Kurazono, Kinnosuke Yahiro, and Joel Moss 2008

[学会発表] Biological activities of Salmonella enterotoxin

Yamasaki E, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, GB Nair, Makino S-I, Hirayama T, Kurazono H 2008

[学会発表] Biological activities of Salmonella enterotoxin.

Yamasaki E, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, G B Nair, Makino S-I, Hirayama T, Kurazono H 2008

[雑誌論文] Molecular typing of Vibrio cholerae O1 isolates from Thailand by pulsed-field gel electrophoresis.

Tapchaisri P, Kurazono H, Chaicumpa W 2008

[雑誌論文] Molecular typing of Vibrio cholerae O1 isolates from Thailand by pulsed-field gel electrophoresis

Tapchaisri, P., Na-Ubol, M., Tiyasuttipan, W., Chaiyaroj, S. C., Yamasaki, S., Wougsaroj, T., Hayashi, H., Nair, G. B., Chougsa-nguan, M., Kurazono, H, Chaimmma, W. 2008

[学会発表] Helicobacter pylori VacA-induced IL-8 production in U937 cells is mediated by an increase in intracellular Ca2+, followed by activation of ATF-2, CREB and NF-kB

Toshiya Hirayama, Jyunzo Hisatsune, Eiki Yamasaki, Hisao Kurazono, Kinnosuke Yahiro, and Joel Moss 2008

[雑誌論文] Molecular characterization of Helicobacter pylori VacA-induction of IL-8 in U937 cells reveals a prominent role for p38MAP kinase in ATF-2, CREB and NF-κB activation

Hisatsune, J., Nakayama, M., Isomoto. H., Kurazono, H., Mukaida, N., Mukhopadhyay, A. K., Sap, J, Yamasaki, E., Yahiro, Y., Moss, J., and Hirayama, T. 2008

[雑誌論文] Molecular characterization of Helicobacter pylori VacA-induction of IL-8 in U937 cells reveals a prominent role for p38MAP kinase in ATF-2, CREB and NF K B activation.

Hisatsune J, Kurazono H, Yamasaki E 2008