耐凍剤チャンネル発現の誘導による卵子・胚の万能凍結保存法の開発

トップ›研究者を探す›耐凍剤チャンネル発現の誘導による卵子・胚の万能凍結保存法の開発

耐凍剤チャンネル発現の誘導による卵子・胚の万能凍結保存法の開発

代表研究者	枝重圭祐
研究課題番号	KAKENHI-PROJECT-20380155
研究種目	基盤研究(B)
研究分野	生物系農学畜産学・獣医学応用動物科学
研究機関	高知大学
研究分担者	葛西孫三郎
研究分担者	越本知大
研究期間開始年月日	2008/4/1
研究期間終了年度	2010
研究ステータス	完了 (2010/4/1)
配分額（合計）	18,720,000 (直接経費 :14,400,000、間接経費 :4,320,000)
配分額（履歴）	2010年度：3,770,000 (直接経費 :2,900,000、間接経費 :870,000) 2009年度：3,900,000 (直接経費 :3,000,000、間接経費 :900,000) 2008年度：11,050,000 (直接経費 :8,500,000、間接経費 :2,550,000)
キーワード	凍結保存卵子胚水透過性耐凍剤透過性アクアポリン受精卵促進拡散

研究成果

[雑誌論文] Cryobiological properties of immature zebrafish oocytes assessed by their ability to be fertilized and develop into hatching embryos

Seki S, Kouya T, Tsuchiya R, Valdez DM Jr, Jin B, Koshimoto C, Kasai M, Edashige K. 2011

[雑誌論文] Developmental ability of vitrified mouse oocytes expressing water channels.

Yohei Yamaji, Shinsuke Seki, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige. 2011

[雑誌論文] Developmental ability of vitrified mouse oocytes expressing water channels

Yamaji Y, Seki S, Matsukawa K, Koshimoto C, Kasai M, Edashige K. 2011

[雑誌論文] Cryobiological properties of immature zebrafish oocytes assessed by their ability to be fertilized and develop into hatching embryos.

Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M.Valdez Jr., Bo Jin, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige. 2011

[図書] 生殖系列細胞の保存.生命の誕生に向けて(第二版)生殖補助医療(ART)培養の理論と実際(日本哺乳動物卵子学会編)

葛西孫三郎, 枝重圭祐 2011

[学会発表] LASセミナー3:胚・精子の凍結保存

中潟直己, 枝重圭祐 2010

[学会発表] Aquaporin 9 facilitates the movement of DMSO and acetamide through the plasma membrane in mouse morulae.

Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Kazutsugu Matsukawa, Chihiro Koshimoto, Magosaburo Kasai 2010

[学会発表] 凍結乾燥したウシ体細胞による核移植胚作出の試み

長尾さや子, 長瀬祐樹, 枝重圭祐, 葛西孫三郎, 保地眞一, 松川和嗣 2010

[学会発表] The role of aquaporin 9 in the movement of Me_2SO and acetamide in mouse morulae

Edashige K, Seki S, Arimura H, Matsuo K, Nakata Y, Jin B, Koshimoto C, Kasai M. 2010

[学会発表] 耐凍剤チャンネル発現の誘導によるマウス卵子の耐凍性向上の試み

和田紗希子, 中田裕一, 金波, 越本知大, 葛西孫三郎, 枝重圭祐 2010

[雑誌論文] Equilibrium vitrification of mouse embryos

Jin B, Mochida K, Ogura A, Hotta E, Kobayashi Y, Ito K, Egawa G, Seki S, Honda H, Edashige K, Kasai M. 2010

[図書] Movement of water and cryoprotectants in mouse oocytes and embryos at different stages : relevance to cryopreservation, In "Fertility Cryopreservation"(Ri-Cheng Chian & Patrick Quinn (Eds.))

Kasai M, Edashige K. 2010

[学会発表] Handy transportation of vitrified mouse embryos with dry ice.

Bo Jin, Keiji Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige 2010

[学会発表] マウスの卵子および胚への耐凍剤透過におけるAquaporin 9の役割

有村隼, 中田裕一, 関信輔, 松尾完, 金波, 松川和嗣, 葛西孫三郎, 枝重圭祐 2010

[学会発表] Handy transportation of vitrified mouse embryos with dry ice

Jin B, Mochida K, Ogura A, Kasai M, Edashige, K. 2010

[学会発表] Aquaporin 9 facilitates the movement of DMSO and acetamide through the plasma membrane in mouse morulae.

Edashige K, Seki S, Arimura H, Matsuo K, Nakata Y, Jin B, Matsukawa K, Koshimoto C, Kasai M. 2010

[図書] 生殖系列細胞の保存(生命の誕生に向けて(第二版)生殖補助医療(ART)培養の理論と実際(日本哺乳動物卵子学会編)

葛西孫三郎, 枝重圭祐 2010

[学会発表] マウスの卵子および胚への耐凍剤透過におけるAquaporin 9の役割

有村隼, 中田裕一, 関信輔, 松尾完, 金波, 松川和嗣, 葛西孫三郎, 枝重圭祐 2010

[図書] Fertility Cryopreservation(Movement of water and cryoprotectants in mouse oocytes and embryos at different stages : relevance to cryopreservation)(Ri-Cheng Chian, Patrick Quinn (Eds.))

葛西孫三郎, 枝重圭祐 2010

[学会発表] 凍結乾燥したウシ体細胞による核移植胚作出の試み

長尾さや子, 長瀬祐樹, 枝重圭祐, 葛西孫三郎, 保地眞一, 松川和嗣 2010

[学会発表] The role of aquaporin 9 in the movement of Me_2SO and acetamide in mouse morulae.

Keisuke Edashige, Shinsuke Seki, Hayato Arimura, Kan Matsuo, Yu-ichi Nakata, Bo Jin, Chihiro Koshimoto, Magosaburo Kasai 2010

[雑誌論文] Equilibrium vitrification of mouse embryos.

Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai. 2010

[学会発表] ブタの卵子と拡大胚盤胞における水と耐凍剤の透過経路

金波, 東山龍一, 米澤潤一, 高橋誠一, 三宅正史, 葛西孫三郎, 枝重圭祐 2009

[学会発表] ドライシッパーを用いないマウス胚の冷蔵及びドライアイス温度下での輸送の検討

持田慶司, 鬼頭靖司, 金波, 枝重圭祐, 葛西孫三郎, 小倉淳郎 2009

[学会発表] Survival of oocytes in antral follicles of vitrified mouse ovaries

Keisuke Edashige 2009

[学会発表] ブクの卵子と拡大胚盤胞における水と耐凍剤の透過経路

金波, 東山龍一, 米澤潤一, 高橋誠一, 三宅正史, 葛西孫三郎, 枝重圭祐 2009

[学会発表] Effect of the expression of aquaporins 1 and 3 in mouse MII oocytes on the nucleation temperature for intracellular ice formation.

Shinsuke Seki, Keisuke Edashige, Peter Mazur 2009

[学会発表] マウス卵巣のガラス化凍結保存

金波, 本田宙, 西門優, 葛西孫三郎, 枝重圭祐 2009

[学会発表] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes -its relevance to their tolerance to cryopreservation-.

Keisuke Edashige, Bo Jin, Ryu-ichi Higashiyama, Jun-ichi Yonezawa, Masashi Miyake, Sei-ichi Takahashi, Ken-ichi Yazawa, Magosaburo Kasai 2009

[学会発表] 様々な発育ステージのガラス化凍結マウス胚の-80℃での短期保持-ドライアイスを用いた簡便な輸送を目指して-

金波, 堀田英里, 小林由希子, 伊藤香, 関信輔, 本田宙, 持田慶司, 小倉淳郎, 葛西孫三郎, 枝重圭祐 2009

[学会発表] Equilibrium vitrification of mouse embryos.

Bo Jin, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Keisuke Edashige, Magosaburo Kasai 2009

[学会発表] Survival of oocytes in antral follicles of vitrified mouse ovaries.

Keisuke Edashige, Delgado M. Valdez Jr., Hiroshi Honda, Yu Nishikado, Bo Jin, Magosaburo Kasai 2009

[学会発表] LASセミナー3:胚・精子の凍結保存

中潟直己, 枝重圭祐 2009

[学会発表] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes -its relevance to their tolerance to cryopreservation-

Keisuke Edashige 2009

[学会発表] The pathway for the movement of water and cryoprotectantsin bovine oocytes and embryos.

Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M. Valdez, Jr., Magosaburo Kasai 2008

[学会発表] 動物細胞と凍結-哺乳動物の卵子と受精卵の耐凍性に関わるタンパク質-

枝重圭祐 2008

[学会発表] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III.

Delgado M. Valdez, Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] The pathway for the movement of water and cryoprotectants in bovine oocytes and embryos.

Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M.Valdez, Jr., Magosaburo Kasai 2008

[雑誌論文] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival.

Bo Jin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai. 2008

[学会発表] Effects of raffinose concentration and methyl-beta-cyclodextrin on IVF with cryopreserved C57BL/6 sperm.

Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura 2008

[学会発表] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio).

Shinsuke Seki, Bo Jin, Tsuchiya Ryouma, Toshimitsu Kouya, Takao Hara, Delgado M.Valdez Jr., Magosaburo Kasai, Keisuke Edashige. 2008

[学会発表] マウス精子が凍結保存によって受ける傷害のメカニズム

好村正博, 金波, 山崎千寛, 山田奈央子, 関信輔, Valdez M.Delgado Jr., 葛西孫三郎, 枝重圭祐 2008

[学会発表] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3 の役割

河合泰典, 原隆夫, 松尾完, Valdez M. Delgado Jr., 金波, 好村正博, 東山龍一, 市川恭子, 恒石望太郎, 枝重圭祐, 葛西孫三郎 2008

[学会発表] Effects of raffinose concentration and methyl-beta cyclodextrin on IVF with cryopreserved C57BL/6 sperm.

Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura 2008

[学会発表] Short-term storage of vitrified two一cell mouse embryos at-80°C for handy transportation with dry ice.

Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji, Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] マウス桑実胚の耐凍剤透過性におけるaquaporin 9の役割

松尾完, 関信輔, 金波, Valdez M. Delgado Jr., 太田悟史, 好村正博, 葛西孫三郎, 枝重圭祐 2008

[学会発表] Short-term storage of vitrified two-cell mouse embryos at -80oC for handy transportation with dry ice.

Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio).

Shinsuke Seki, Bo Jin, Tsuchiya Ryouma, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] マウス桑実胚の耐凍剤透過性におけるaquaporin 9の役割

松尾完, 関信輔, 金波, Valdez M.Delgado Jr., 太田悟史, 好村正博, 葛西孫三郎, 枝重圭祐 2008

[学会発表] 動物細胞と凍結-哺乳動物の卵子と受精卵の耐凍性に関わるタンパク質-

枝重圭祐 2008

[雑誌論文] Development of a reliable in vitro maturation system for zebrafish oocytes.

Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M.Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige. 2008

[学会発表] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III.

Delgado M.Valdez Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige 2008

[雑誌論文] Development of a reliable in vitro maturation system forzebrafi sh oocytes.

Shinsuke Seki, Toshimitsu Kouya, Ryoraa Tsuchiya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] マウス精子が凍結保存によって受ける傷害のメカニズム.

好村正博, 金波, 山崎千寛, 山田奈央子, 関信輔, Valdez M. Delgado Jr., 葛西孫三郎, 枝重圭祐 2008

[学会発表] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3の役割

河合泰典, 原隆夫, 松尾完, Valdez M.Delgado Jr., 金波, 好村正博, 東山龍一, 市川恭子, 恒石望太郎, 枝重圭祐, 葛西孫三郎 2008

[雑誌論文] The mechanism by which mouse spermatozoa are injured during freezing.

Bo Jin, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige. 2008

[雑誌論文] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival.

Bo Tin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai 2008