トップ研究者を探す実験用小型淡水魚類卵子の凍結保存:耐凍剤チャンネルの人為的発現によるアプローチ

実験用小型淡水魚類卵子の凍結保存:耐凍剤チャンネルの人為的発現によるアプローチ

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-19300147
研究種目 基盤研究(B)
研究分野 総合・新領域系
総合領域
実験動物学
実験動物学
研究機関 高知大学
代表研究者 葛西 孫三郎
研究分担者 枝重 圭祐
研究分担者 越本 知大
研究期間 開始年月日 2007/4/1
研究期間 終了年度 2009
研究ステータス 完了 (2009/4/1)
配分額(合計) 18,460,000 (直接経費 :14,200,000、間接経費 :4,260,000)
配分額(履歴) 2009年度:4,550,000 (直接経費 :3,500,000、間接経費 :1,050,000)
2008年度:4,290,000 (直接経費 :3,300,000、間接経費 :990,000)
2007年度:9,620,000 (直接経費 :7,400,000、間接経費 :2,220,000)
キーワード 凍結保存
魚類卵子
水チャンネル
ゼブラフィッシュ
細胞膜透過性
魚類
卵子
水透過性
耐凍剤透過性
膜透過性
尿素輸送体

研究成果

[雑誌論文] Equilibrium vitrification of mouse embryos

Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai 2010

[図書] Movement of water and cryoprotectants in mouse oocytes and embryos at different stages: relevance to cryopreservation

Magosaburo Kasai, Keisuke Edashige 2010

[学会発表] Equilibrium vitrification of mouse embryos

Bo Jin, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Keisuke Edashige, Magosaburo Kasai 2009

[学会発表] ブタの卵子と拡大胚盤胞における水と耐凍剤の透過経路

金 波, 東山 龍一, 米澤 潤一, 高橋 誠一, 三宅 正史, 葛西 孫三郎, 枝重 圭祐 2009

[学会発表] Survival of oocytes in antral follicles of vitrified mouse ovaries

Keisuke Edashige 2009

[学会発表] Effect of the expression of aquaporins 1 and 3 in mouse MII oocytes on the nucleation temperature for intracellular ice formation

Shinsuke Seki, Keisuke Edashige, Peter Mazur 2009

[学会発表] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes-its relevance to their tolerance to cryopreservation-

Keisuke Edashige, Bo Jin, Ryu-ichi Higashiyama, Jun-ichi Yonezawa, Masashi Miyake, Sei-ichi Takahashi, Ken-ichi Yazawa, Magosaburo Kasai 2009

[学会発表] Survival of oocytes in antral follicles of vitrified mouse ovaries

Keisuke Edashige, Delgado M. Valdez Jr., Hiroshi Honda, Yu Nishikado, Bo Jin, Magosaburo Kasai 2009

[学会発表] Rapid movement of water and cryoprotectants in pig expanded blastocystsvia channel processes -its relevance to their tolerance to cryopreservation-

Keisuke Edashige 2009

[学会発表] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III

Delgado M. Valdez. Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] Effects of raffinose concentration and methyl-beta-cyclodextrin on IVF with cryopreserved C57BL/6 sperm

Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura 2008

[雑誌論文] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival

Bo Jin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai 2008

[雑誌論文] Development of a reliable in vitro maturation system for zebrafish oocytes

Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] Effects of raffinose concentration and methyl-beta-cyclodextrin on IVF with cryopreserved C57BL/6 sperm.

Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura 2008

[学会発表] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio).

Shinsuke Seki, Bo Jin, Tsuchiya Ryouma, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] マウス桑実胚の耐凍剤透過性におけるaquaporin 9の役割

松尾 完, 関 信輔, 金 波, Valdez M. Delgado Jr., 太田悟史, 好村 正博, 葛西 孫三郎, 枝重 圭祐 2008

[学会発表] The pathway for the movement of water and cryoprotectants in bovine oocytes and embryos

Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M. Valdez, Jr., Magosaburo Kasai 2008

[雑誌論文] Development of a reliable in vitro maturation system for zebrafish oocytes.

Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2008

[雑誌論文] The mechanism by which mouse spermatozoa are injured during freezing.

Bo Jin, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III.

Delgado M. Valdez, Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] Short-term storage of vitrified two-cell mouse embryos at-80℃ for handy transportation with dry ice.

Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji, Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige 2008

[学会発表] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3の役割

河合 泰典, 原 隆夫, 松尾完, Valdez M. Delgado Jr., 金 波, 好村 正博, 東山 龍一, 市川 恭子, 恒石 望太郎, 枝重 圭祐, 葛西 孫三郎 2008

[学会発表] 動物細胞と凍結-哺乳動物の卵子と受精卵の耐凍性に関わるタンパク質-

枝重圭祐 2008

[学会発表] マウス精子が凍結保存によって受ける傷害のメカニズム.

好村 正博, 金 波, 山崎 千寛, 山田 奈央子, 関 信輔, Valdez M. Delgado Jr., 葛西 孫三郎, 枝重 圭祐 2008

[雑誌論文] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival.

Bo Jin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai 2008

[学会発表] The pathway for the movement of water and cryoprotectants in bovine oocytes and embryos.

Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M. Valdez, Jr., Magosaburo Kasai 2008

[雑誌論文] The mechanism by which mouse spermatozoa are injured during freezing

Bo Jin, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige 2008

[図書] Vitrification in animal reproduction:vitrification of embryos using conventional straws with an ethylene glycol-based solutions.Vitrification in Assisted Reproduction:A User's Manual and Trouble-Shooting Guide.(Michael J.Tucker and Juergen Liebermann, eds.),75-85

Magosaburo Kasai, Keisuke Edashige 2007

[学会発表] The role of urea transporters in the movement of cryoprotectants across the plasma membrane in mouse morulae

Keisuke Edashige, Satoshi Ohta, Tomomi Kajino, Tatsunaga Kuwano, Bo Jin, Delgado M. Valdez Jr., Shinsuke Seki, Magosaburo Kasai 2007

[学会発表] Simple technique to improve motility and fertility in frozen C57BL/6J mouse sperm

Chihiro Koshimoto, Yuuki Schichi, Keisuke Edashige, Magosaburo Kasai, Keiji Mochida, Akio Shinohara 2007

[雑誌論文] The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish(Danio rerio)

Shinsuke Seki, Toshimitsu Kouya, Delgado M.Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2007

[学会発表] マウス精子の凍結保存による傷害

金 波, 山崎 千寛, 山田 奈央子, 関 信輔, Valdez M.Delgado Jr., 葛西 孫三郎, 枝重 圭祐 2007

[雑誌論文] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae

Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai 2007

[雑誌論文] The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio)

Shinsuke Seki, Toshimitsu Kouya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2007

[学会発表] Simple technique to improve motility and fertility in frozen C57BL/6J mouse sperm

Chihiro Koshimoto, Yuuki Schichi, Keisuke Edashige, Magosaburo Kasai, Keiji Mochida, Akio Shinohara 2007

[学会発表] The role of urea transporters in the movement of cryoprotectants across th eplasma membrane in mouse morulae

Keisuke Edashige, Satishi Ohta, Tomomi Kajino, Tatsunaga Kuwano, Bo Jin, Delgado M.Valdez Jr., Shinsuke Seki, Magosaburo Kasai 2007

[学会発表] Assessment of the cryoprotectant permeability and cryoprotectant toxicity of aquaporin 3-expressing immature medaka (Oryzias latipes) oocytes

Delgado M. Valdez Jr., Seki Shinsuke, Takao Hara, Naoya Saida, Yu Nishikado, Kasai Magosaburo, Edashige Keisuke 2007

[雑誌論文] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish(Danio rerio)

Shinsuke Seki, Toshimitsu Kouya, Takao Hara, Delgado M.Valdez Jr., Bo Jin, Magosaburo Kasai, Keisuke Edashige 2007

[雑誌論文] The movement of water and cryoprotectants in mammalian oocytes and embryos and its relevance to cryopreservation

Keisuke Edashige, Magosaburo Kasai 2007

[学会発表] The role of aquaporin-3 in the movement of water and cryoprotectants in mouse morulae

Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo. Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai 2007

[図書] Vitrification in animal reproduction: vitrification of embryos using conventional straws with an ethylene glycol-based solutions

Magosaburo Kasai, Keisuke Edashige 2007

[学会発表] Assessment of the cryoprotectant permeability and cryoprotectant toxicity of aquaporin 3-expressing immature medaka(Oryzias latipes)oocytes

Delgado M.Valdez Jr., Shinsuke Seki, Takao Hara, Naoya Saida, Yu Nishikado, Magosaburo Kasai, Keisuke Edashige 2007

[学会発表] ryobiological properties of immature oocytes assessed by the ability to be fertilized and to develop to term

Shinsuke Seki, Kouya Toshimitsu, Ryoma Tsuchiya, Delgado M Valdez Jr., Bo Jin, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2007

[雑誌論文] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae

Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M.Valdez Jr., Takao Hara, Bo Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magsaburo Kasai. 2007

[学会発表] Intracellular ice formation in vitrified mouse morulae during warming

Bo Jin, Kenji Kusanagi, Makiko Ueda, Keisuke Edashige, Magosaburo Kasai 2007

[学会発表] Cryobiological properties of immature oocytes assessed by the ability to be fertilized and to develop to term

Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M.Valdez Jr., Bo Jin, Naoya Saida, Magosaburo Kasai, Keisuke Edashige 2007

[雑誌論文] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio)

Shinsuke Seki, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr., Bo Jin, Magosaburo Kasai, Keisuke Edashige 2007