ヌクレアーゼ抵抗性修飾核酸を搭載した多機能性ナノ構造体による新規核酸医薬の創製

トップ›研究者を探す›ヌクレアーゼ抵抗性修飾核酸を搭載した多機能性ナノ構造体による新規核酸医薬の創製

ヌクレアーゼ抵抗性修飾核酸を搭載した多機能性ナノ構造体による新規核酸医薬の創製

代表研究者	松田彰
研究課題番号	KAKENHI-PROJECT-18109001
研究種目	基盤研究(S)
研究分野	生物系医歯薬学薬学創薬化学
研究機関	北海道大学
研究分担者	原島秀吉
研究分担者	南川典昭
研究期間開始年月日	2006/4/1
研究期間終了年度	2010
研究ステータス	完了 (2010/4/1)
配分額（合計）	108,160,000 (直接経費 :83,200,000、間接経費 :24,960,000)
配分額（履歴）	2010年度：18,850,000 (直接経費 :14,500,000、間接経費 :4,350,000) 2009年度：18,850,000 (直接経費 :14,500,000、間接経費 :4,350,000) 2008年度：18,850,000 (直接経費 :14,500,000、間接経費 :4,350,000) 2007年度：18,850,000 (直接経費 :14,500,000、間接経費 :4,350,000) 2006年度：32,760,000 (直接経費 :25,200,000、間接経費 :7,560,000)
キーワード	ヌクレアーゼ抵抗性 4'-チオ核酸 siRNA ベクター薬物送達システムヌクレアーゼ抵抗性核酸 2'-O-メチル-4'-チオリポヌクレオシド SNALP MEND miRNA コレステロール Apo-B 2'-O-メチル-4'-チオリボヌクレオシド 4'-チオDNA ホタルルシフェラーゼ Dicer リポソームゲノム創薬 RNA干渉ヌクレアーゼ祇抗性核酸 NMR si RNA antisense RNA 2'-0-メチル-4'-チオリボヌクレオシドアプタマー RNAi 膜透過性ペプチドマクロピノサイトーシス 2'-0-メチル-4'-チオリポヌクレオシドマクロビノサイトーシス

研究成果

[雑誌論文] Convenient RNA synthesis using a phosphoramidite possessing a biotinylated photocleavable group.

Tomaya K.; Takahashi M.; Minakawa N.; Matsuda A. 2010

[雑誌論文] Selective recognition of unnatural imidazopyridopyrimidine : naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment.

Minakawa N.; Ogata S.; Takahashi M.; Matsuda A. 2009

[雑誌論文] Synthesis and characterization of 2'-modified-4'-thioRNA : a comprehensive comparison of nuclease stability

Takahashi, M. ; Minakawa, N. ; Matsuda, A. 2009

[雑誌論文] Synthesis and characterization of 2'-modified-4'-thioRNA : a comprehensive comparison of nuclease stability.

Takahashi M.; Minakawa N.; Matsuda A. 2009

[雑誌論文] Selective recognition of unnatural imidazopyridopyrimidine : naphthyridine base pairs consisting of four hydrogen bonds by the Klenow fragment.

N.Minakawa, et al. 2009

[雑誌論文] Unnatural imidazopyridopyrimidine : naphthyridine base pairs : selective incorporation and extension reaction by Deep Vent (exo-) DNA polymerase.

Ogata S.; Takahashi M.; Minakawa N.; Matsuda A. 2009

[雑誌論文] Oligonucleotides containing 3-bromo-3-deazaadenine and 7-bromo-7-deazaadenine 2'-deoxynucleosides as chemical probes to investigate DNA-protein interactions.

Minakawa N.; Kawano Y.; Murata S.; Inoue N.; Matsuda A. 2008

[雑誌論文] Investigations toward the selection of fully-modified 4'-thioRNA aptamers : optimization of in vitro transcription step in the presence of 4'-thioNTPs

Minakawa, N. ; Sanji, M. ; Kato, Y. ; Matsuda, A. 2008

[雑誌論文] Unexpected A-form formation of 4'-thioDNA in solotion, as revealed by NMR, and the implication on the mechanism of nuclease-resistance

Matsugami, A. ; Ohyama, T. ; Inada, M. ; Inoue, N. ; Minakawa, N. ; Matsuda, A. ; Katahira, M. 2008

[学会発表] 核酸化学からアプタマーへ : ヌクレアーゼ抵抗性の化学的調節

松田彰、加藤優佳、三次未央子、高橋真由美、南川典昭 2008

[雑誌論文] Investigations toward the selection of fullymodified 4'-thioRNA aptamers : optimization of in vitro transcription step in the presence of 4'-thioNTPs.

Minakawa N.; Sanji M.; Kato Y.; Matsuda A. 2008

[雑誌論文] Synthesis ans crystal structure of 2'-deoxy-2'-fluoro-4'-thioribonucleosides : substrates for the synthesis of novel modified RNAs.

Takahashi M.; Daidouji S.; Shiro M.; Minakawa N.; Matsuda A. 2008

[雑誌論文] Investigations toward the selection of fully-modified 4'-thioRNA aptamers: optimization of in vitro transcription step in the presence of 4'-thio NTPs

N. Minakawa, et.al. 2008

[雑誌論文] Ohgonucleotides containing 3 bromo-3-deazaadenine and 7-bromo-7-deazaadenine 2'-deoxynucleosides as chemical probes to investigate DNA-protein interactions

N. Minakawa, et.al. 2008

[雑誌論文] Unexpected A-form formation of 4'-thioDNA in solotion, as revealed by NMR, and the implication on the mechanism of nucleaseresistance.

Matsugami A.; Ohyama T.; Inada M.; Inoue N.; Minakawa N.; Matsuda A.; Katahira M. 2008

[図書] スーパー核酸 : 4'-チオ核酸の合成から核酸医薬開発に向けた基礎研究の展開,化学フロンテイア「創薬をめざす有機合成戦略 : 進化する医薬品づくり」(宍戸, 宏造, 新藤, 充)

南川典昭, 松田彰 2007

[雑誌論文] Amplification of 4'-thioDNA in the presence of 4'-thio-Dttp and 4'-thio-dCTP, and 4'-thioDNA-directed transcription in vitro and in mammalian cells

Inoue, N. ; Shionoya, A. ; Minakawa, N. ; Ogawa, N. ; Matsuda, A. 2007

[雑誌論文] Study of modification pattern-RNAi activity relationships using siRNAs modified with 4'-thioribonucleosides, finding an effective modification irrespective of target sequence.

Hoshika S.; Minakawa N.; Shionoya A.; Imada K.; Ogawa N.; Matsuda A. 2007

[雑誌論文] Amplification of 4'-thioDNA in the presence of 4'-thio-dTTP and 4'-thio-dCTP, and 4'-thioDNA-directed transcription in vitro and in mammalian cells.

Inoue N.; Shionoya A.; Minakawa N.; Ogawa N.; Matsuda A. 2007

[学会発表] Amplification of 4'-thioDNA and its utility as a siRNA expression.

南川典昭 2007

[図書] 核酸医薬の創製をめざして : 「バイオとナノの融合II,新生命科学への応用」(北海道大学COE研究成果編集委員会)

松田彰, 南川典昭 2007

[雑誌論文] Oligonucleotides containing 3-bromo-3-deazaadenine and 7-bromo-7-deazaadenine 2'-deoxynucleosides as chemical probes to investigate DNA-protein interactions.

N. Minakawa, et al. 2007

[雑誌論文] Synthesis and properties of 4'-thioDNA: unexpected RNA-like behavior of 4'-thioDNA.

Inoue N.; Minakawa N.; Matsuda A. 2006

[雑誌論文] Synthesis and properties of 4'-thioDNA : unexpected RNA-like behavior of 4'-thioDNA

Inoue, N. ; Minakawa, N. ; Matsuda, A. 2006

[雑誌論文] Synthesis and characterization of oligodeoxynucleotides containing naphthyridine : imidazopyridopyrimidine base pairs at their sticky ends. Application as thermally stabilized decoy molecules.

Hikishima S.; Minakawa N.; Kuramoto K.; Ogata S.; Matsuda A. 2006