トップ›研究者を探す›染色体工学技術とゲノム編集技術の融合による遺伝子改変ブタの作製

染色体工学技術とゲノム編集技術の融合による遺伝子改変ブタの作製

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-17H03938
研究種目 基盤研究(B)
研究分野 生物系
農学
動物生命科学
統合動物科学
研究機関 徳島大学
代表研究者 音井 威重
研究分担者 竹本 龍也
研究分担者 香月 康宏
研究分担者 谷原 史倫
研究期間 開始年月日 2017/4/1
研究期間 終了年度 2019
研究ステータス 完了 (2019/4/1)
配分額（合計） 16,250,000 (直接経費 :12,500,000、間接経費 :3,750,000)
配分額（履歴） 2019年度：5,070,000 (直接経費 :3,900,000、間接経費 :1,170,000)
2018年度：5,070,000 (直接経費 :3,900,000、間接経費 :1,170,000)
2017年度：6,110,000 (直接経費 :4,700,000、間接経費 :1,410,000)
キーワード ゲノム編集
人工染色体
ブタ
異種移植
GalT
HLA A24
染色体工学

研究成果

[雑誌論文] Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.

Namula Z, Tanihara F, Wittayarat M, Hirata M, Nguyen NT, Hirano T, Le QA, Nii M, Otoi T. 2019

[雑誌論文] Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.

Hirata M, Tanihara F, Wittayarat M, Hirano T, Nguyen NT, Le QA, Namula Z, Nii M, Otoi T. 2019

[雑誌論文] Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos

Tanihara F, Hirata M, Nguyen NT, LE QA, Hirano T, Otoi T. 2019

[雑誌論文] Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid.

Thi Nguyen N, Hirata M, Tanihara F, Hirano T, Le QA, Nii M, Otoi T. 2019

[雑誌論文] Relationship among ovarian follicular status, developmental competence of oocytes, and anti-Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.

Tanihara F, Hirata M, Iizuka S, Sairiki S, Nii M, Nguyen NT, Le QA, Hirano T, Otoi T. 2019

[学会発表] エレクトロポレーションを用いたCRISPR/Cas9システムのブタ体外受精卵への導入によるCD163遺伝子改変ブタの作製

谷原史倫、平田真樹、Nguyen Thi Nhien、Quynh Anh Le、平野隆之、音井威重 2019

[学会発表] Effect of Cas9 protein levels on genomic mutations using the gene editing by electroporation of Cas9 protein (GEEP) system in putative zygotes.

Le A.Q,, Hirata, M., Tanihara, F., Nguyen, T.N., Hirano, T. and Otoi T. 2019

[学会発表] Assessment of PDX-1-deficient pigs generated using the CRISPR/Cas9 system introduced into porcine zygotes via electroporation.

Tanihara, F., Hirata, M., Nguyen, T.N., Le A.Q,, Hirano, T. and Otoi T. 2019

[雑誌論文] Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes.

Zhao Namula, Manita Wittayarat, Maki Hirata, Takayuki Hirano, Nhien Thi Nguyen, Quynh Anh Le, Mokhamad Fahrudin, Fuminori Tanihara, Takeshige Otoi 2019

[学会発表] Effects of CRISPR/Cas9-mediated gene targeting of porcine endogenous retrovirus on the developmental competence of porcine embryos.

Hirata, M., Tanihara, F., Nguyen, T.N., Le A.Q,, Hirano, T. and Otoi T. 2019

[学会発表] Efficiency of gene editing by electroporation of Cas9 protein (GEEP) to generate GGTA1-modified pigs.

Nguyen, T.N., Hirata, M., Tanihara, F., Le A.Q,, Hirano, T. and Otoi T. 2019

[雑誌論文] Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.

Nguyen TV, Tanihara F, Hirata M, Hirano T, Nishio K, Kim Do LT, Nguyen TV, Nii M, Otoi T. 2018

[学会発表] Concentration of CRISPR/Cas9 components effects on genetic mosaicism of cytoplasmic microinjected porcine embryos

Le Anh Quynh, Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano and Takeshige Otoi 2018

[学会発表] ブタ内在性レトロウイルス遺伝子を標的としたゲノム編集が胚発育能に及ぼす影響

谷原史倫、平田真樹、Nguyen Thi Nhien、平野隆之、音井威重 2018

[学会発表] Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid

Nguyen Thi Nhien, Fuminori Tanihara, Maki Hirata, Takayuki Hirano, Le Anh Quynh, Masahiro Nii and Takeshige Otoi 2018

[雑誌論文] Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.

Nguyen TV, Wittayarat M, Do LTK, Nguyen TV, Nii M, Namula Z, Kunihara T, Tanihara F, Hirata M, Otoi T. 2018

[雑誌論文] Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm.

Namula Z, Hirata M, Wittayarat M, Tanihara F, Thi Nguyen N, Hirano T, Nii M, Otoi T. 2018

[雑誌論文] Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.

Hirata M, Tanihara F, Taniguchi M, Takagi M, Terazono T, Otoi T. 2018

[学会発表] ゲノム編集によるTP53遺伝子改変ブタの作製と表現型の解析

谷原 史倫, 平田 真樹, Nguyen Thi Nhien, Le Anh Quynh, 平野 隆之, 竹本 龍也, 中井 美智子, 淵本 大一郎, 音井 威重 2018

[学会発表] ブタにおける受精前後でのゲノム編集が胚盤胞の変異導入効率に及ぼす影響,

平田 真樹, 谷原 史倫, 平野 隆之, Nguyen Thi Nhien, Le Anh Quynh, 新居 雅宏, 音井 威重 2018

[雑誌論文] Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.

Nishio K, Tanihara F, Nguyen TV, Kunihara T, Nii M, Hirata M, Takemoto T, Otoi T. 2018

[学会発表] ブタ体外受精卵におけるCrispr/Cas9システムを使用したゲノム編集の効率.

平田 真樹、谷原 史倫、Nhien Thi Nguyen、Zhao Namula、音井威重 2018

[学会発表] Modification of SALL1 gene via CRISPR/Cas9-mediated gene editing introduced into porcine zygotes by electroporation

Tanihara, F., Takemoto, T., Hirata, M., Nguyen, T.V., Kunihara,T. Nishinakamura, R. and Otoi, T. 2018