水圏生物の核内受容体CARを利用した化学物質のハイスループットリスク評価

トップ›研究者を探す›水圏生物の核内受容体CARを利用した化学物質のハイスループットリスク評価

水圏生物の核内受容体CARを利用した化学物質のハイスループットリスク評価

代表研究者	岩田久人
研究課題番号	KAKENHI-PROJECT-17208030
研究種目	基盤研究(A)
研究分野	生物系農学境界農学環境農学
研究機関	愛媛大学
研究分担者	金恩英
研究分担者	田辺信介
研究期間開始年月日	2005/4/1
研究期間終了年度	2008
研究ステータス	完了 (2008/4/1)
配分額（合計）	43,940,000 (直接経費 :33,800,000、間接経費 :10,140,000)
配分額（履歴）	2008年度：8,840,000 (直接経費 :6,800,000、間接経費 :2,040,000) 2007年度：9,880,000 (直接経費 :7,600,000、間接経費 :2,280,000) 2006年度：9,750,000 (直接経費 :7,500,000、間接経費 :2,250,000) 2005年度：15,470,000 (直接経費 :11,900,000、間接経費 :3,570,000)
キーワード	constitutive androstane receptor PCB DDT POPs アザラシ核内レセプター CAR 難分解性有機汚染物質水圏生物シグナル伝達リスク評価

研究成果

[雑誌論文] Effects of co-exposure to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin and perfluorooctane sulfonate or perfluorooctanoic acid on expression of cytochrome P450 isoforms in chicken(Gallus gallus)embryo hepatocyte cultures

Watanabe, M. X., Iwata, H., et al. 2009

[雑誌論文] Accumulation features and temporal trends of PCDDs, PCDFs and PCBs in Baikal seals(Pusa sibirica)

Imaeda, D., Iwata, H., et al. 2009

[雑誌論文] Alkoxyresorufin(methoxy-, ethoxy-, pentoxy- and benzyloxyresorufin)O-dealkylase activities by in vitro expressed cytochrome P450 1A4 and 1A5 from common cormorant(Phalacrocorax carbo)

Kubota, A., Kim, E. Y., Iwata, H. 2009

[図書] 第5章生物反応を利用して化学物質汚染の危険性を検知する分子で読む環境汚染, 鈴木聡編

岩田久人, 金恩英 2009

[雑誌論文] Dioxin activation of CYP1A5 promoter/enhancer regions from two avian species, common cormorant ( Phalacrocorax carbo) and chicken ( Gallus gallus) : association with aryl hydrocarbon receptor 1 and 2 isoforms

Lee, J.S., Kim, E.Y., Iwata, H. 2009

[学会発表] Contamination status of brominated flame retardants (BFRs)in Baikal seals(Pusas sibirica).

Isobe, T., Iwata, H., et al. 2008

[雑誌論文] Contamination and effects of perfluorochemicals in Baikal seal (Pusa sibirica). 1. Residue level, tissue distribution, and temporal trend.

Ishibashi, H., Iwata, H., et al. 2008

[雑誌論文] Contamination and effects of perfluorochemicals in Baikal seal. 2. molecular characterization, expression level, and transcriptional activation of peroxisome proliferator-activated receptor α

Ishibashi, H., Iwata, H., Kim, E.Y., 他5名 2008

[学会発表] バイカルアザラシCYP2BおよびCYP3Aプロモーター/エンハンサー領域の分子特性とPOPsによる転写活性化能

酒井大樹・岩田久人・金恩英・Petrov, E. A.・田辺信介 2008

[学会発表] Low Induction Potencies of Cytochrome P450 2B and 3A by Persistent Organic Pollutants in Baikal Seal ( Pusa sibirica)

Sakai, H., Iwata, H., Kim, E.Y., Petrov, E.A. and Tanabe, S. 2008

[学会発表] Relative Potencies of Individual Dioxin-Like Compounds for In Vitro Cytrochrome P450 1A1 Transactivation of Aryl Hydrocarbon Receptor from Baikal Seal

Iwata, H., Kim, E.Y., Suda, T., Tanabe, S. and Petrov, E.A. 2008

[学会発表] Effects of dioxins in wild Baikal seals : an implication from a toxicogenomic approach.

Hirakawa, S., Iwata, H., et al. 2008

[学会発表] ニワトリ新規AHR1アイソフォーム、2nd AHR1, in chicken (Gallusgallus) : mRNA expression profile andtranscriptional potency bydioxins.

岩渕康平, 金恩英, 安井知子, 岩田久人 2008

[雑誌論文] Dioxin activation of CYP1A5 promoter/enhancer regions from two avian species, common cormorant(Phalacrocorax carbo)and chicken (Gallus gallus) : Association with aryl hydrocarbon receptor 1 and 2 isoforms.

Lee, J. S., Kim, E. Y., Iwata, H. 2008

[学会発表] Contamination status of brominated flame retardants (BFRs) in Baikal seals (Pusa sibirica).

Isobe, T., Iwata, H., et al. 2008

[学会発表] Molecular characterization and functional analysis of aryl hydrocarbon receptor 1 and 2 (AHR1 and AHR2) isoforms from jungle crow (Corvus macrorhynchos).

Inoue, N., Kim, E. Y., Iwata, H. 2008

[学会発表] A novel aryl hydrocarbon receptpr isoform, 2^<nd> AHRl, in chicken(Gallus gallus) ; isolation and functional characterization.

Iwabuchi, K., Iwata, H., et al. 2008

[学会発表] Functional characterization of in vitro - expressed Baikal Seal cytochrome P450 1A1.

Yamaguchi, K., Kim, E. Y., Iwata, H. 2008

[学会発表] Effects of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD)on morphogenesis in developing red seabream(Pagrus major)embryo.

Iida, M., Iwata, H., et al. 2008

[学会発表] Accumulation characteristics of hydroxylated polychlorinated biphenyl congeners in the blood of marine mammals and human.

Nomiyama, K., Iwata, H., et al. 2008

[学会発表] Effects of in ovo exposure of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin on AHR-CYPIA signaling pathway in common cormorant.

Nagahama, N., Iwata, H., et al. 2008

[学会発表] Effects of dioxins in wild Baikal seals : an implication from a toxicogenomic approach

Hirakawa, S., Imaeda, D., Nakayama, K., Kim, E. Y., Kunisue, T., Tanabe, S., Petrov, E. A., Batoev, V. B. and Iwata, H. 2008

[学会発表] ダイオキシン類を暴露させたカワウ肝由来培養細胞における遺伝子発現変動の比較

仲山慶, 酒井大樹, 北村真一, 須藤明子, 金恩英, 岩田久人, 田辺信介 2008

[学会発表] Molecular characterization of Baikal seal CYP2B/3A promoter/enhancer regions and their transactivation potencies by POPs

Sakai, H., Iwata, H., Kim, E. Y., Petrov, E. A. and Tanabe, S. 2008

[学会発表] Molecular characterization of the aryl hydrocarbon receptor repressor(AHRR)from chicken(Gallus gallus).

Lee, J. S., Kim, E. Y., Iwata, H. 2008

[学会発表] Proposal of species-specific 2, 3, 7, 8-TCDD-TEF for wildlife toxicology : relative potencies for AHR-mediated responses of dioxin-like chemicals in Baikal seal and jungle crow.

Kim, E. Y., Iwata, H., et al. 2008

[雑誌論文] Contamination and effects of perfluorochemicals in Baikal seal (Pusa sibirica). 2. Molecular characterization, expression level, and transcriptional activation of peroxisome proliferator-activated receptor a.

Ishibashi, H., Iwata, H., et al. 2008

[雑誌論文] Catalytic function of avian cytochrome P450 1A4 and 1A5 (CYP1A4 and CYP1A5) enzymes heterologously expressed using in vitro yeast system

Kubota, A., Iwata, H. and Kim, E.Y. 2008

[学会発表] TCDD曝露したマダイ胚の形態学的異常 : アクアポリンおよびCYP3Aの発現量変化と神経系の撹乱.

飯田緑, 金恩英, 村上安則, 島康洋, 岩田久人 2008

[学会発表] バイカルアザラシCYP2BおよびCYP3Aプロモーター/エンハンサー領域の分子特性とPOPsによる転写活性化能.

酒井大樹, 岩田久人, 金恩英, Petrov, E. A., 田辺信介 2008

[学会発表] AHR-CYP1A signaling pathway in birds : inter-species and -isoform comparisons.

Kim, E. Y., Iwata, H., et al. 2008

[雑誌論文] Toxicogenomic analysis of immune system-related genes in Japanese flounder(Paralichthya olivaceus)exposed to heavy oil.

Nakayama, K., Iwata, H., et al. 2008

[学会発表] Molecular characterization of Baikal seal CYP2B/3A promoter/enhancer regions and their transactivation potencies by POPs.

Sakai, H., Iwata, H., et al. 2008

[学会発表] Residue levels of OH-PCBs and PCBs in the blood of Baikal seals(Pusa sibirica).

Imaeda, D., Iwata, H., et al. 2008

[学会発表] Hydroxylated polychlorinated biphenyls in the blood of Baikal seals (Pusa sibirica).

Imaeda, D., Iwata, H., et al. 2008

[雑誌論文] Potential effects of perfluorinated compounds in common cormorants from Lake Biwa, Japan : an implication from the hepatic gene expression profiles by microarray.

Nakayama, K., Iwata, H., et al. 2008

[学会発表] in vitro発現させたバイカルアザラシシトクロムP4501A1の機能特性.

山口恵佑, 金恩英, 岩田久人 2008

[雑誌論文] Contamination and effects of perfluorochemicals in Baikal seal. 1. residue level, tissue distribution, and temporal trend

Ishibashi, H., Iwata, H., Kim, E.Y., 他7名 2008

[学会発表] Searching dioxin-responsive genes and mechanisms of dioxin effects in the liver of Baikal seals. Interdisciplinary Symposium on Environmental Sciences.

Hirakawa, S., Iwata, H., et al. 2008

[図書] 第5章生物反応を利用して化学物質汚染の危険性を検知する, 分子で読む環境汚染

岩田久人・金恩英 2008

[雑誌論文] Chemical contamination in aquatic ecosystems

Iwata, H., Kim, E.Y., 他4名 2007

[学会発表] Species diversity of AHR-CYP1A signaling pathways : Toward risk assessment of dioxin-like compounds in wildlife

Iwata, H. and Kim, E. Y. 2007

[雑誌論文] Identification and hepatic expression profiles of cytochrome P450 1-4 isozymes in common minke whales (Balaenoptera acutrostrata)

Niimi, S., Kim, E.Y., Iwata, H., et. al. 2007

[雑誌論文] Toxic effects of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) in developing red seabream (Pagrus major) embryo : an association of morphological deformities with AHR1, AHR2 and CYP1A expressions

Yamauchi, M., Kim, E.Y., Iwata, H., Shima, Y. and Tanabe, S. 2007

[雑誌論文] Molecular characterlzation of cytochrome P450 1A1,1A2, and 1B1,and effects of polychlorinated dibenzo-p-dioxin,dibenzofuran,and biphenyl congeners on their hepatic expression

Hirakawa, S., Iwata, H., Kim, E.Y., et. al. 2007

[学会発表] Xenobiotic ligand profiles of constitutive androstane receptor (CAR) and pregnane X receptor (PXR) in Baikal seal ( Pusa sibirica)

Iwata, H., Sakai, H., Kadota, M., Kim, E. Y., Tanabe, S., Batoev, V. B. and Petrov, E. A 2007

[雑誌論文] Functional characterization and evolutionary history of two aryl hydrocarbon receptor isoforms (AhR1 and AhR2) from Avian Species

Yasui, T., Kim, E.Y., Iwata, H., 他4名 2007

[雑誌論文] Functional characterization and evolutionary history of two aryl hydrocarbon receptor isoforms (AhR1 and AhR2) from avian species

Yasui, T., Kim, E.Y., Iwata, H., et. al. 2007

[雑誌論文] Molecular characterization of cytochrome P450 1A1, 1A2, and 1B1, and effects of polychlorinated dibenzo- p-dioxin, dibenzofuran, and biphenyl congeners on their hepatic expression in Baikal seal

Hirakawa, S., Iwata, H., Takeshita, Y., Kim, E.Y., 他6名 2007

[学会発表] Xenobiotic ligand profiles of constitutive androstane receptor(CAR)and pregnane X receptor(PXR)in Baikal seal(Pusa sibirica)

Iwata, H., et. al. 2007

[雑誌論文] Identification and hepatic expression profiles of cytochrome P450 1-4 isozymes in common minke whales

Niimi, S., Kim, E.Y., Iwata, H., Watanabe, M.X., 他3名 2007

[雑誌論文] Molecular characterization and tissue distribution of aryl hydrocarbon receptor nuclear translocator isoforms, ARNT1 and ARNT2, and identification of novel splice variants in common cormorant (Phalacrocorax carbo)

Lee, J.S., Kim, E.Y., Iwata, H., Tanabe, S. 2007

[学会発表] Inter-species and -isoform differences in signaling pathways of aryl hydrocarbon receptors : A casestudy of fish and bird

Iwata, H. and Kim, E. Y. 2007

[学会発表] Contamination by perfluorinated compounds and effects on peroxisome proliferators-activated receptor alpha-Cytochrome P450 4A signaling pathway in Baikal seal ( Pusa sibirica).

Iwata, H., Ishibashi, H., Kim, E. Y., Tao, L., Kannan, K., Tanabe, S., Batoev, V. B. and Petrov, E. A. 2007

[学会発表] Transactivation potentials of constitutive androstane/active receptor by persistent organic pollutants and brominated flameretardants : a comparative study of Baikal Seal ( Pusa sibirica) and mouse.

Sakai, H., Iwata, H., Kim, E.Y., Petrov, E. A. and Tanabe, S. 2007

[雑誌論文] Congener-specific toxicokinetics of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and coplanar polychlorinated biphenyls in black-eared kites : cytochrome P4501A-dependent hepatic sequestration

Kubota, A., Iwata, H., 他3名 2006

[雑誌論文] PCDDs, PCDFs, and coplanar PCBs in wild terrestrial mammals from Japan : congener specific accumulation and hepatic sequestration

Kunisue, T., Watanabe, M.X., Iwata, H., 他4名 2006

[雑誌論文] Constitutive androstane receptor (CAR) as a potential sensing biomarker of persistent organic pollutants (POPs) in aquatic mammal : molecular characterization, expression level and ligand profiling in Baikal seal

Sakai, H., Iwata, H., Kim, E.Y., 他5名 2006

[雑誌論文] Cytochrome P450 1A4 and 1A5 in common cormorant (Phalacrocorax carbo) : evolutionary relationships and functional implications associated with dioxin and related compounds

Kubota, A., Iwata, H., Goldstone, H. M. H., Kim, E. Y., Stegeman, J. J. and Tanabe, S. 2006

[図書] 化学物質の生態リスク評価と規制-農薬編-

岩田久人 2006

[図書] 日本環境毒性学会, 畠山成久編, アイピーシー

岩田久人 2006

[雑誌論文] Induction of cytochrome P450 1A5 mRNA, protein and enzymatic activities by dioxin-like compounds, and congener-specific metabolism and sequestration in the liver of wild jungle crow from Tokyo, Japan

Watanabe, M.X., Iwata, H., Kim, E.Y., 他3名 2005

[雑誌論文] Aryl hydrocarbon receptor (AHR) and AHR nuclear translocator expression in Baikal seal and association with 2,3,7,8-TCDD toxic equivalents and CYP1 expression levels

Kim, E.Y., Iwata, H., 他5名 2005

[雑誌論文] Molecular cloning and mRNA expression of cytochrome P4501A1 and 1A2 in the liver of common minke whales ( Balaenoptera acutorostrata)

Niimi, S., Watanabe, M.X., Kim, E.Y., Iwata, H., Yasunaga, G., Fujise, Y. and Tanabe, S. 2005

[雑誌論文] Hepatic CYP1A induction by dioxin-like compounds, and congener-specific metabolism and sequestration in wild common cormorants from Lake Biwa Japan.

Kubota, A., Iwata, H., Tanabe, S., Yoneda, K. and Tobata, S. 2005

[雑誌論文] Molecular characterization of the aryl hydrocarbon receptors (AHR1 and AHR2) from red seabream ( Pagrus major)

Yamauchi, M., Kim, E.Y., Iwata, H. and Tanabe, S. 2005

[雑誌論文] Bioaccumulation of organochlorines in crows from Indian open waste dumping site : evidence for direct transfer of dioxin-like congeners from the contaminated soil

Watanabe, M.X., Iwata, H., 他3名 2005

[雑誌論文] Bioaccumulation of organochlorines in crows from an indian open waste dumping site : evidence for direct transfer of dioxin-like congeners from the contaminated soil

Watanabe, M.X. 2005

[雑誌論文] Induction of cytochrome P450 1A5 mRNA, protein and enzymatic activities by dioxin-like compounds, and congener-specific metabolism and sequestration in the liver of wild jungle crow (Corvus macrorhynchos) from Tokyo, Japan.

Watanabe, M.X. 2005