トップ研究者を探す試験管内タンパク質合成の分子基盤と細胞機能模倣に向けたその応用

試験管内タンパク質合成の分子基盤と細胞機能模倣に向けたその応用

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-17076011
研究種目 特定領域研究
研究分野
研究機関 独立行政法人産業技術総合研究所
徳島大学
代表研究者 片岡 正俊
研究分担者 篠原 康雄
研究分担者 石田 竜弘
研究分担者 森 秀治
研究期間 開始年月日 2005/4/1
研究期間 終了年度 2009
研究ステータス 完了 (2009/4/1)
配分額(合計) 82,100,000 (直接経費 :82,100,000)
配分額(履歴) 2009年度:16,100,000 (直接経費 :16,100,000)
2008年度:16,100,000 (直接経費 :16,100,000)
2007年度:16,100,000 (直接経費 :16,100,000)
2006年度:16,900,000 (直接経費 :16,900,000)
2005年度:16,900,000 (直接経費 :16,900,000)
キーワード 膜タンパク質
リポソーム
試験管内タンパク質合成
コートペプチド
細胞機能模倣
再構成
高次構造
フォールディング
抗体
プロテインA
細胞機能の模倣
マイクロチップ電気泳動
ナノバイオデバイス
合成ペプチド
マイクロファブリケーション

研究成果

[雑誌論文] Replacement of C305 in heart/muscle-type isozyme of human carnitine palmitoyltransferase I with aspartic acid and other amino acids

Matsuo T, Yamamoto A, Yamamoto T, Otsuki K, Yamazaki N, Kataoka M, Terada H, Shinohara Y. 2010

[雑誌論文] Modulation of calprotectin in human keratinocytes by keratinocyte growth factor and interleukin-1alpha

Bando M, Hiroshima Y, Kataoka M, Herzberg MC, Ross KF, Shinohara Y, Yamamoto T, Nagata T, Kido J. 2010

[雑誌論文] Analysis of DNA ligation by microchip electrophoresis

Umemoto Y, Kataoka M, Yatsushiro S, Yamamura S, Ooie T, Kido J, Yamamoto T, Shinohara Y, Baba Y. 2010

[雑誌論文] Ca2+-induced permeability transition can be observed even in yeast mitochondria under optimized experimental conditions

Yamada A, Yamamoto T, Yoshimura Y, Gouda S, Kawashima S, Yamazaki N, Yamashita K, Kataoka M, Nagata T, Terada H, Pfeiffer DR, Shinohara Y 2009

[雑誌論文] Sequential analysis of RNA synthesis by microchip electrophoresis

Umemoto Y, Kataoka M, Yatsushiro S, Watanabe M, Kido J, Kakuhata R, Yamamoto T, Shinohara Y, Baba Y. 2009

[雑誌論文] Direct endonuclease digestion and multi-analysis of restriction fragment length polymorphisms by microchip electrophoresis

Akamine R, Yatsushiro S, Yamamura S, Kido J, Shinohara Y, Baba Y, Kataoka M. 2009

[雑誌論文] Differential permeabilization effects of Ca2+ and valinomycin on the inner and outer mitochondrial membranes as revealed by proteomics analysis of proteins released from mitochondria

Yamada A, Yamamoto T, Yamazaki N, Yamashita K, Kataoka M, Nagata T, Terada H, Shinohara Y. 2009

[雑誌論文] Classification of FABP isoforms and tissues based on quantitative evaluation of transcript levels of these isoforms in various rat tissues

Yamamoto T, Yamamoto A, Watanabe M, Matsuo T, Yamazaki N, Kataoka M, Terada H, Shinohara Y. 2009

[雑誌論文] Substitution of certain amino acids in a short peptide causes a significant difference in their immunoreactivities with antibodies against different epitopes: evidence for possible folding of the peptide on a nitrocellulose or PVDF membrane

Matsuo T, Yamamoto T, Katsuda C, Niiyama K, Yamamoto A, Yamazaki N, Ohkura K, Kataoka M, Shinohara Y. 2009

[雑誌論文] Construction of plasmids suitable for in vitro synthesis of full-length mRNAs having a 3'-poly (A)+tail

Matsuo T, Obana E, Yamamoto T, Kakuhata R, Yamazaki N, Kataoka M, Baba Y, Hori T, Shinohara Y. 2009

[雑誌論文] Distinct behaviors of adenylate kinase and cytochrome c observed following induction of mitochondrial permeability transition by Ca(2+) in the absence of respiratory substrate

Yamamoto T, Yoshimura Y, Yamada A, Gouda S, Yamashita K, Yamazaki N, Kataoka M, Nagata T, Terada H, Shinohara Y. 2008

[雑誌論文] Cold-induced changes in gene expression in brown adipose tissue: implications for the activation of thermogenesis

Watanabe M, Yamamoto T, Mori C, Okada N, Yamazaki N, Kajimoto K, Kataoka M, Shinohara Y. 2008

[雑誌論文] Functionally important conserved length of C-terminal regions of yeast and bovine ADP/ATP carriers, identified by deletion mutants studies, and water accessibility of the amino acids at the C-terminal region of the yeast carrier

Iwahashi A, Ishii A, Yamazaki N, Hashimoto M, Ohkura K, Kataoka M, Majima E, Terada H, Shinohara Y. 2008

[雑誌論文] Importance of probe location for quantitative comparison of signal intensities among genes in microarray analysis

Kakuhata R, Watanabe M, Yamamoto T, Obana E, Yamazaki N, Kataoka M, Ooie T, Baba Y, Hori T, Shinohara Y. 2008

[雑誌論文] Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli, wheat germ, and rabbit reticulocytes

Hino M, Kataoka M, Kajimoto K, Yamamoto T, Kido J, Shinohara Y, Baba Y. 2008

[雑誌論文] Accurate quantitation of salivary and pancreatic amylase activities in human plasama by microchip electrophoretic separation of the substrates and hydrolysates coupled with immunoinhibition

Maeda E, Kataoka M, Yatsushiro S, Kajimoto K, Hino M, Kaji N, Tokeshi M, Bando M, Kido J, Ishikawa M, Shinohara Y, Baba Y. 2008

[雑誌論文] Synchronized changes in transcript levels of genes activating cold exposure-induced thermogenesis in brown adipose tissue of experimental animals

Watanabe M, Yamamoto T, Kakuhata R, Okada N, Kajimoto K, Yamazaki N, Kataoka M, Baba Y, Tamaki T, Shinohara Y. 2008

[雑誌論文] Usefulness of the 5' region of the cDNA encoding acidic ribosomal phosphoprotein P0 conserved among rats, mice, and humans as a standard probe for gene expression analysis in different tissues and animal species

Akamine R, Yamamoto T, Watanabe M, Yamazaki N, Kataoka M, Ishikawa M, Ooie T, Baba Y, Shinohara Y. 2007

[雑誌論文] Regulation of calprotectin expression by interleukin-1alpha and transforming growth factor-beta in human gingival keratinocytes

Hayashi N, Kido J, Kido R, Wada C, Kataoka M, Shinohara Y, Nagata T. 2007

[図書] バイオセンサ・ケミカルセンサ辞典、第III編第2章第2節キャピラリー型DNA検知システム

片岡 正俊、篠原 康雄 2007

[雑誌論文] Interleukin-1alpha regulates antimicrobial peptide expression in human keratinocytes

Bando M, Hiroshima Y, Kataoka M, Shinohara Y, Herzberg MC, Ross KF, Nagata T, Kido J. 2007

[図書] キャピラリー型DNA検知システム

片岡正俊、篠原康雄 2007

[雑誌論文] Possible utilization of in vitro synthesized mRNAs specifically expressed in certain tissues as standards for quantitative evaluation of the results of microarray analysis

Kakuhata R, Watanabe M, Yamamoto T, Akamine R, Yamazaki N, Kataoka M, Fukuoka S, Ishikawa M, Ooie T, Baba Y, Hori T, Shinohara Y. 2007

[雑誌論文] Label-free detection of protein-protein interactions at the GaAs/water interface through surface infrared spectroscopy: discrimination between specific and nonspecific interactions by using secondary structure analysis

Onodera K, Hirano-Iwata A, Miyamoto K, Kimura Y, Kataoka M, Shinohara Y, Niwano M. 2007

[雑誌論文] Determination of human blood glucose levels using microchip electrophoresis

Maeda E, Kataoka M, Hino M, Kajimoto K, Kaji N, Tokeshi M, Kido J, Shinohara Y, Baba Y. 2007

[雑誌論文] VDAC1, having a shorter N-terminus than VDAC2 but showing the same migration in an SDS-polyacrylamide gel, is the predominant form expressed in mitochondria of various tissues

Yamamoto T, Yamada A, Watanabe M, Yoshimura Y, Yamazaki N, Yoshimura Y, Yamauchi T, Kataoka M, Nagata T, Terada H, Shinohara Y 2006

[雑誌論文] High-turnover osteoporosis is induced by cyclosporin A in rats

Wada C, Kataoka M, Seto H, Hayashi N, Kido J, Shinohara Y, Nagata T. 2006

リポソームに抗体結合能を付与するペプチド、および当該ペプチドで修飾されたリボソーム

リポソームに抗体結合能を付与するペプチド、および当該ペプチドで修飾されたリポソーム