トップ研究者を探す水チャンネル発現の制御による卵子の凍結保存-哺乳動物から無脊椎動物まで-

水チャンネル発現の制御による卵子の凍結保存-哺乳動物から無脊椎動物まで-

KAKEN 科学研究費助成事業データベース で見る
研究課題番号 KAKENHI-PROJECT-16380190
研究種目 基盤研究(B)
研究分野 生物系
農学
畜産学・獣医学
応用動物科学
研究機関 高知大学
代表研究者 葛西 孫三郎
研究分担者 枝重 圭祐
研究分担者 越本 知大
研究期間 開始年月日 2004/4/1
研究期間 終了年度 2006
研究ステータス 完了 (2006/4/1)
配分額(合計) 15,000,000 (直接経費 :15,000,000)
配分額(履歴) 2006年度:3,100,000 (直接経費 :3,100,000)
2005年度:2,900,000 (直接経費 :2,900,000)
2004年度:9,000,000 (直接経費 :9,000,000)
キーワード アクアポリン
水透過性
耐凍剤透過性
凍結保存
卵子
マウス
魚類
昆虫
細胞膜透過性
ガラス化
卵子の凍結保存
アフリカツメガエル
メダカ
キョウソヤドリコバチ
aquaporin
water permeability
cryoprotectant permeability
cryopreservation
oocyte
mouse
fish
insect

研究成果

[雑誌論文] The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio).

S.Seki, T.Kouya, D.M.Valdez Jr., B.Jin, T.Hara, N.Saida, M.Kasai, K.Edashige. 2007

[雑誌論文] The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish(Danio rerio)

S.Seki, T.Kouya, D.M.Valdez Jr., B.Jin, T.Hara, N.Saida, M.Kasai, K.Edashige 2007

[雑誌論文] Mazur Extra-and intra-cellular ice formation in stage I and II Xenopus laevis oocytes.

J.F.Guenther, S.Seki, F.W.Kleinhnas, K.Edashige, D.M.Roberts, P. 2006

[雑誌論文] Japanese flounder (Paralichthys olivaceus) embryos are difficult to be cryopreserved by vitrification

K.Edashige et al. 2006

[雑誌論文] Expression of aquaporin-3 improves the permeability to water and cryoprotectants of immature oocytes in the medaka (Oryzias latipes).

D.M.Valdez Jr., T.Hara, A.Miyamoto, S.Seki, B.Jin, M.Kasai, K.Edashige 2006

[雑誌論文] Channel-dependent permeation of water and glycerol in mouse morulae

K.Edashige et al. 2006

[雑誌論文] Japanese flounder (Paralichthys olivaceus) embryos are difficult to be cryopreserved by vitrification.

K.Edashige, D.M.Valdez Jr., T.Hara, N.Saida, S.Seki, M.Kasai. 2006

[雑誌論文] Channel-dependent permeation of water and glycerol in mouse morulae

Keisuke Edashige, Mitsunobu Tanaka, Natsuko Ichimaru, Satoshi Ota, Ken-ich, Yazawa, Yuko Higashino, Megumi Sakamoto, Yohei Yamaji, TATSUNAGA Kuwano, Delgado M.Valdez Jr., F.W.Kleinhans, Magosaburo Kasai 2006

[雑誌論文] Channel-dependent permeation of water and glycerol in mouse morulae.

K.Edashige, M.Tanaka, N.Ichimaru, S.Ota, K.Yazawa, Y.Higashino, M.Sakamoto, Y.Yamaji, T.Kuwano, D.M Valdez Jr., F.W.Kleinhans, M.Kasai. 2006

[雑誌論文] Extra-and intra-cellular ice formation in stage IandIIXenopus laevis oocytes

J.F.Guenther, S.Seki, F.W.Kleinhnas, K.Edashige, D.M.Roberts, P.Mazur 2006

[雑誌論文] Channel-dependent permeation of water and glycerol in mouse morulae

K.Edashige, M.Tanaka, N.Ichimaru, S.Ota, K.Yazawa, Y.Higashino, M.Sakamoto, Y.Yamaji, T.Kuwano, D.M.Valdez Jr., F.W.Kleinhans, M.Kasai 2006

[雑誌論文] Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes.

Y.Yamaji, D.M.Valdez Jr., S.Seki, K.Yazawa, C.Urakawa, B.Jin, M.Kasai, F.W.Kleinhans, K.Edashige. 2006

[雑誌論文] Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes

Y.Yamaji, D.M.Valdez Jr., S.Seki, K.Yazawa, C.Urakawa, B.Jin, M.Kasai, F.W.Kleinhans, K.Edashige 2006

[雑誌論文] Expression of aquaporin-3 improves the permeability to water and cryoprotectants of immature oocytes in the medaka(Oryzias latipes)

D.M.Valdez Jr., T.Hara, A.Miyamoto, S.Seki, B.Jin, M.Kasai, K.Edashige 2006

[雑誌論文] Japanese flounder (Paralichthys olivaceus) embryos are difficult to be cryopreserved by vitrification

K.Edashige, D.M.Valdez Jr., T.Hara, N.Saida, S.Seki, M.Kasai 2006

[雑誌論文] Sensitivity to chilling of medaka (Oryzias latipes) embryos at various developmental stages.

Valdez DM Jr, Miyamoto A, Hara T, Edashige K, Kasai M 2005

[雑誌論文] Sensitivity to chilling of medaka (Oryzias latipes) embryos at various developmental stages

Delgado M.Valdez Jr., Akira Miyamoto, Takao Hara, Keisuke Edashige, Magosaburo Kasai 2005

[雑誌論文] Extra- and intracellular ice formation in mouse oocytes

Peter Mazur, Shinsuke Seki, Irina L.Pinn, F.W.Kleinhans, Keisuke Edashige 2005

[雑誌論文] Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants

Prudencio B.Pedro, Eiji Yokoyama, Shi En Zhu, Naoko Yoshida, Delgado M.Valdez Jr., Mitsunobu Tanaka, Keisuke Edashige, Magosaburo Kasai 2005

[雑誌論文] Effect of hold time after extracellular ice formation on intracellular freezing of mouse oocytes.

P.Mazur, I.L.Pinn, S.Seki, F.W.Kleinhans, K.Edashige. 2005

[雑誌論文] Water- and cryoprotectant-permeability of mature and immature oocytes in the medaka (Oryzias latipes).

Valdez, DM Jr, Miyamoto A, Hara T, Seki S, Kasai M, Edashige K 2005

[雑誌論文] Extra-and intracellular ice formation in mouse oocytes.

P.Mazur, S.Seki, I.L.Pinn, F.W.Kleinhans, K.Edashige 2005

[雑誌論文] Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants.

P.B.Pedro, E.Yokoyama, S.E.Zhu, N.Yoshida, D.M.Valdez Jr., M.Tanaka, K.Edashige, M.Kasai. 2005

[雑誌論文] Water-and cryoprotectant-permeability of mature and immature oocytes in the medaka (Oryzias latipes).

D.M.Valdez Jr., A.Miyamoto, T.Hara, S.Seki, M.Kasai, K.Edashige. 2005

[雑誌論文] Water- and cryoprotectant-permeability of mature and immature oocytes in the medaka (Oryzias latipes)

Delgado M.Valdez Jr., Akira Miyamoto, Takao Hara, Shinsuke Seki, Magosaburo Kasai, Keisuke Edashige 2005

[雑誌論文] Sensitivity to chilling of medaka (Oryzias latipes) embryos at various developmental stages

D.M.Valdez Jr., A.Miyamoto, T.Hara, K.Edashige, M.Kasai. 2005

[雑誌論文] Effect of hold time after extracellular ice formation on intracellular freezing of mouse oocytes

Peter Mazur, Irina L.Pinn, Shinsuke Seki, F.W.Kleinhans, Keisuke Edashige 2005

[雑誌論文] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae

K.Edashige et al.

[雑誌論文] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio).

S.Seki, T.Kouya, T.Hara, D.M.Valdez Jr., B.Jin, M.Kasai, K.Edashige.

[雑誌論文] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish(Danio rerio)

S.Seki, T.Kouya, T.Hara, D.M.Valdez Jr., B.Jin, M.Kasai, K.Edashige

[雑誌論文] Extra- and intra-cellular ice formation in stage I and II Xenopus laevis oocytes

James F.Guenther, Shinsuke Seki, F.W.Kleinhnas, Keisuke Edashige, Daniel M.Roberts, Peter Mazur

[雑誌論文] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae.

K.Edashige, S.Ohta, M Tanaka, T.Kuwano, D.M.Valdez Jr., T.Hara, B.Jin, S.Takahashi, S.Seki, C.Koshimoto, M.Kasai.