レーザーを駆使した単一細胞内反応場の構築と計測

トップ›研究者を探す›レーザーを駆使した単一細胞内反応場の構築と計測

代表研究者	増原宏
研究課題番号	KAKENHI-PROJECT-16072211
研究種目	特定領域研究
研究分野
研究機関	大阪大学
研究分担者	朝日剛
研究分担者	開祐司
研究分担者	今元泰
研究期間開始年月日	2004/4/1
研究期間終了年度	2006
研究ステータス	完了 (2006/4/1)
配分額（合計）	135,200,000 (直接経費 :135,200,000)
配分額（履歴）	2006年度：36,000,000 (直接経費 :36,000,000) 2005年度：45,100,000 (直接経費 :45,100,000) 2004年度：54,100,000 (直接経費 :54,100,000)
キーワード	顕微光散乱分光単一生細胞フェムト秒レーザーマウスNIH3T3細胞デキストラン蛍光イメージングアクチンストレスファイバー EGFP修飾単一細胞アクチンフィラメント緑色蛍光蛋白質衝撃波形態変化アクチィンフィラメント Light Scattering Microspectroscopy Single Bio Cell Femtosecond Laser Mousr NIH3T3 Cell Dextran Fluorescence Imaging Actin Stress Fiber EGFP Modification

研究成果

[雑誌論文] Single Particle Spectroscopic Investigation on the Interaction between Exciton Transition of Cyanine Dye J-Aggregates and Localized Surface Plasmon Polarization of Gold Nanoparticles

Takayuki Uwada, Ryo Toyota, Hiroshi Masuhara, Tsuyoshi Asahi 2007

[雑誌論文] Multipole Resonance Modes in Localized Surface Plasmon of Single Hexagonal/Triangular Gold Nanoplate

Takayuki Uwada, Tsuyoshi Asahi, Hiroshi Masuhara, Daisuke Ibano, Manabu Fujishiro, Toshihiro Tominaga 2007

[雑誌論文] Multipole Resonance Modes in Localized Surface Plasmon of Single Hexagonal /Triangular Gold Nanoplates

Takayuki Uwada, Tsuyoshi Asahi, Hiroshi Masuhara, Daisuke Ibano, Manabu Fujishiro, Toshihiro Tominaga 2007

[雑誌論文] Single Particle Spectroscopic Investigation on the Interaction between Exciton Transition of Cyanine Dye J-Aggregates and Localized Surface Plasmon Polarization of Gold Nanoparticles

Takayuki Uwada, Ryo Toyota, Hiroshi Masuhara, Tsuyoshi Asahi 2007

[雑誌論文] Realignment Process of Actin Stress Fibers in Single Living Cells Studied by Focused Femtosecond Laser Irradiation

Ryohei Yasukuni, Jean-Alexis Spitz, Rachel Meallet-Renault, Takayuki Negishi, Takuji Tada, Yoichiro Hosokawa, Tsuyoshi Asahi, Chisa Shukunami, Yuji Hiraki, Hiroshi Masuhara 2007

[図書] Nano Biophotonics : Science and Technology (第15,16,22,24章)

Hiroshi Masuhara, Tsuyoshi Asahi, Yuuji Hiraki, et al. 2007

[雑誌論文] Multipole Resonance Modes in Localized Surface Plasmon of Single Hexagonal / Triangular Gold Nanoplates

Takayuki Uwada, Tsuyoshi Asahi, Hiroshi Masuhara, Daisuke Ibano, Manabu Fujishiro, Toshihiro Tominaga 2007

[雑誌論文] Polarization and Wavelength Dependent Nonlinear Optical Properties of a Photo-Switchable Organic Crystal

Michel Sliwa, Keitaro Nakatani, Tsuyoshi Asahi, Pascal G. Lacroix, Robert B. Pansu, Hiroshi Masuhara 2007

[雑誌論文] Size and Phase Control in Quinacridone Nanoparticle Formation by Laser Ablation in Water

Teruki Sugiyama, Tsuyoshi Asahi, Hiroki Takeuchi, Hiroshi Masuhara 2006

[学会発表] Photocurrent Measurement of Quinacridone Films Consisting of Size-Controlled Nanoparticles Prepared by Laser Ablation in Water

Hyeon-Gu Jeon, Tsuyoshi Asahi, Teruki Sugiyama, Hiroshi Masuhara 2006

[学会発表] Light-Driven Proton Pump in Halobacterial Cells

Kazuo Tsujimoto, Taku Mizukami, Takayuki Negishi, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[学会発表] Ultrafast Laser Spectroscopy of Photochromic Compounds in Microcrystalline Phase (Invited)

Tsuyoshi Asahi 2006

[雑誌論文] Higher-order Multiphoton Imaging by Femtosecond Near-Infrared Laser Microscope System

Hirohisa Matsuda, Syoji Ito, Yutaka Nagasawa, Tsuyoshi Asahi, Hiroshi Masuhara, Seiya Kobatake, Masahiro Irie, Hiroshi Miyasaka 2006

[雑誌論文] Time-resolved thermodynamics : Heat capacity change of transient species during photoreaction of PYP

Javaid Shahbaz Khan, Yasushi Imamoto, Mikio Kataoka, Fumio Tokunaga, Masahide Terazima 2006

[雑誌論文] Size and Phase Control in Quinacridone Nanoparticle Formation by Laser Ablation in Water

Teruki Sugiyama, Tsuyoshi Asahi, Hiroki Takeuchi, Hiroshi Masuhara 2006

[学会発表] Development Femtosecond Near-Infrared Microscope and Higher-Order Multiphoton Imaging

Hirohisa Matsuda, Syoji Ito, Yutaka Nagasawa, Hiroshi Miyasaka, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[学会発表] Polarization and Wavelength Dependence of Second Harmonic Generation in a Photo-Switchable Crystal

Michel Sliwa, Keitaro Nakatani, Robert Pansu, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[雑誌論文] Laser Nano Chemistry

Hiroshi Masuhara, Tsuyoshi Asahi, Yoichiroh Hosokawa 2006

[雑誌論文] Laser Nano Chemistry

Hiroshi Masuhara, Tsuyoshi Asahi, Yoichiroh Hosokawa 2006

[雑誌論文] Conformational Changes of PYP Monitored by Diffusion Coefficient : Effect of N-terminal □-Helices

Javaid Shahbaz Khan, Yasushi Imamoto, Miki Harigai, Mikio Kataoka, Masahide Terazima 2006

[雑誌論文] Spectroscopy and Photochemistry of Single Organic Nanocrystals Investigated by Using a Far-Eield Optical Microscope Coupled with an AFM System

Tsuyoshi Asahi, Victor V.Volkov, Hideki Matsune, Hidetsugu Kawai, Hiroshi Masuhara 2006

[雑誌論文] Spectroscopy and Photochemistry of Single Organic Nanocrystals Investigated by Using a Far-Eield optical Microscope Coupled with an AFM System

Tsuyoshi Asahi, Victor V. Volkov, Hideki Matsune, Hidetsugu Kawai, Hiroshi Masuhara 2006

[雑誌論文] Trans-cis Photoisomerization of a Photoactive Yellow Protein Model Chromophore in Crystalline Phase

Anwar Usman, Hiroshi Masuhara, Tsuyoshi Asahi 2006

[雑誌論文] Size and Phase Control in Quinacridone Nanoparticle Formation by Laser Ablation in Water

Teruki Sugiyama, Tsuyoshi Asahi, Hiroki Takeuchi, Hiroshi Masuhara 2006

[雑誌論文] Conformational Changes of PYP Monitored by Diffusion Coefficient : Effect of N-terminal α-Helices

Javaid Shahbaz Khan, Yasushi Imamoto, Miki Harigai, Mikio Kataoka, Masahide Terazima 2006

[雑誌論文] Laser Nano Chemistry

Hiroshi Masuhara, Tsuyoshi Asahi, Yoichiroh Hosokawa 2006

[学会発表] Excited State Isomerization of a PYP Model Chromophore in Crystalline Solid Phase

Anwar Usman, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[雑誌論文] Higher-order Multiphoton Imaging by Femtosecond Near-Infrared Laser Microscope System

Hirohisa Matsuda, Syoji Ito, Yutaka Nagasawa, Tsuyoshi Asahi, Hiroshi Masuhara, Seiya Kobatake, Masahiro Irie, Hiroshi Miyasaka 2006

[学会発表] Spectroscopy and Photochemistry of Single Organic Nanoparticles (Invited)

Hiroshi Masuhara, Tsuyoshi Asahi 2006

[図書] Nanoplasmonics : From Fundamentals to Applications

Hiroshi Masuhara, Tsuyoshi Asahi, Hiroyuki Yoshikawa, et al. 2006

[雑誌論文] Spectroscopy and Photochemistry of Single Organic Nanocrystals Investigated by Using a Far-Eield Optical Microscope Coupled with an AFM System

Tsuyoshi Asahi, Victor V. Volkov, Hideki Matsune, Hidetsugu Kawai, Hiroshi Masuhara 2006

[学会発表] Development of a Confocal Rayleigh Scattering Spectroscopic Imaging System and Its Application to Single Gold Nanoparticles

Takayuki Uwada, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[雑誌論文] Temperature Dependence of Ultrafast Photoinduced Ring-opening and -closure Reactions of Spironaphthooxazine in Crystalline Phase

Mototsugu Suzuki, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[学会発表] Femtosecond Laser-induced Ablation and Contraction Dynamics of Actin Filaments in Mouse NIH3T3 Fibroblast Cells

Ryohei Yasukuni, Yoichiroh Hosokawa, Tsuyoshi Asahi, Yuji Hiraki, Hiroshi Masuhara 2006

[学会発表] "Spectroscopy and Photochemistry of Single Organic Nanoparticles" (Invited)

Hiroshi Masuhara, Tsuyoshi Asahi 2006

[学会発表] Realignment Process of Actin Stress Fiber Dissected by Focused Femtosecond Laser

Ryohei Yasukuni, Jean-Alexis Spitz, Rachel Meallet-Renault, Yoichiroh Hosokawa, Tsuyoshi Asahi, Chisa Shukunami, Yuji Hiraki, Hiroshi Masuhara 2006

[雑誌論文] Trans-cis Photoisomerization of a Photoactive Yellow Protein Model Chromophore in Crystalline Phase

Anwar Usman, Hiroshi Masuhara, Tsuyoshi Asahi 2006

[雑誌論文] Higher-order Multiphoton Imaging by Femtosecond Near-Infrared Laser Microscope System

Hirohisa Matsuda, Syoji Ito, Yutaka Nagasawa, Tsuyoshi Asahi, Hiroshi Masuhara, Seiya Kobatake, Masahiro Irie, Hiroshi Miyasaka 2006

[雑誌論文] Time-resolved thermodynamics : Heat capacity change of transient species during photoreaction of PYP

Javaid Shahbaz Khan, Yasushi Imamoto, Mikio Kataoka, Fumio Tokunaga, Masahide Terazima 2006

[学会発表] "Ultrafast Laser Spectroscopy of Photochromic Compounds in Microcrystalline Phase" (Invited)

Tsuyoshi Asahi 2006

[学会発表] Size-dependent Optical and Photophysical Properties of Polydiacetylene Nanocrystal

Tsuyoshi Asahi, Victor V. Volkov, Hiroshi Masuhara 2006

[雑誌論文] Conformational Changes of PYP Monitored by Diffusion Coefficient: Effect of N-terminal α-Helices

Javaid Shahbaz Khan, Yasushi Imamoto, Miki Harigai, Mikio Kataoka, Masahide Terazima 2006

[学会発表] Fluorescence Microspectroscopic Measurement on Intracellular pH in Single Living Halobacteria

Takayuki Negishi, Go Tanaka, Yoichiro Hosokawa, Tsuyoshi Asahi, Hirosh Masuhara, Taku Mizukami, Kazuo Tsujimoto 2006

[雑誌論文] Temperature Dependence of Ultrafast Photoinduced Ring-opening and -closure Reactions of Spironaphthooxazine in Crystalline Phase

Mototsugu Suzuki, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[学会発表] Preparation of Fullerene C60 Dispersion by Laser Ablation in Poor Solvents

Teruki Sugiyama, Sen-ichi Ryou, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[学会発表] fs-Time-Resolved Diffuse Reflectance Spectroscopic Studies on Dynamics of Surface Charge-Carriers of Trans-tetratoluyl Porphyrin Tin Hydroxide (SnTTP)-Incorated Titania Nanotubes

Minjoong Yoon, Ki Seok Jeon, Joon Hee Jang, Tsuyoshi Asahi, Hiroshi Masuhara 2006

[雑誌論文] Trans-cis Photoisomerization of a Photoactive Yellow Protein Model Chromophore in Crystalline Phase

Anwar Usman, Hiroshi Masuhara, Tsuyoshi Asahi 2006

[学会発表] Dissection of Biological Filaments by Femtosecond Laser Irradiation

Ryohei Yasukuni, Yoichiroh Hosokawa, Tsuyoshi Asahi, Yuji Hiraki, Hiroshi Masuhara 2006

[学会発表] Realignment Process of Actin Stress Fiber Dissected by Focused Femtosecond Laser

Ryohei Yasukuni, Jean-Alexis Spitz, Rachel Meallet-Renault, Yoichiroh Hosokawa, Tsuyoshi Asahi, Chisa Shukunami, Yuji Hiraki, Hiroshi Masuhara 2006

[雑誌論文] 光の散乱を捉え粒子の形状を見るレイリー散乱分光法

朝日剛 2005

[学会発表] Femtosecond Time-Resolved Diflectance Spectroscopy of Titania and SnTTP-Incorporated Titania Nanotubes: Dynamics of Surface Charge-Carriers

Ki Seok Jeon, Joon Hee Jang, Tsuyoshi Asahi, Hiroshi Masuhara, MingJoon Yoon 2005

[学会発表] Femtosecond laser-induced melting of anthracene crystal studied by ultrafast microscopy (Invited)

Tsuyoshi Asahi, Hiroshi Masuhara 2005

[学会発表] Coherent Coupling of J-aggregate Exciton to Surface Plasmon Electronic Oscillations of Single Au Nanoparticles (Invited)

Tsuyoshi Asahi, Ryo Toyota, Takayuki Uwada, Hiroshi Masuhara 2005

[学会発表] Single Particle Spectroscopic Investigation on Gold Nanoparticles Coverd with Cyanine Dye J-aggregate

Takayuki Uwada, Ryo Toyota, Tsuyoshi Asahi, Hiroshi Masuhara 2005

[雑誌論文] A biosensor in the time domain based on the diffusion coefficient measurement : intermolecular interaction of an intermediate of photoactive yellow protein

Javaid Shahbaz Khan, Yasushi Imamoto, Yoichi Yamazaki, Mikio Kataoka, Fumio Tokunaga, Masahide Terazima 2005

[雑誌論文] Development of Near-Infrared 35 fs Laser Microscope and Its Application to the Detection of Three- and Four-Photon Fluorescence of Organic Microcrystals

Hirohisa Matsuda, Yousuke Fujimoto, Syoji Ito, Yutaka Nagasawa, Hiroshi Miyasaka, Tsuyoshi Asahi, Hiroshi Masuhara 2005

[学会発表] Spectroscopic and Photochemical Studies on Single Organic Nanoparticles (Invited)

Hiroshi Masuhara, Tsuyoshi Asahi 2005

[雑誌論文] Single Molecule Spectroscopy of Organic Dye Nanoparticles

Andre J. Gesquiere, Takayuki Uwada, Tsuyoshi Asahi, Hiroshi Masuhara, Paul F. Barbara 2005

[雑誌論文] Design, Synthesis, Structural and Nonlinear optical Properties of Photochromic Crystals: Towards Reversible Molecular Switches

Michel Sliwa, Sylvie Letard, Isabelle Malfant, Martine Nierlich, Pascal G. Lacroix, Tsuyoshi Asahi, Hiroshi Masuhara, Pei Yu, Keitaro Nakatani 2005

[雑誌論文] Photochromic Dynamics of Salicylidene Aniline in Solid State by Using Femtosecond Transient Absorption Spectroscopy

T. Asahi, H. Masuhara, K. Nakatani, M. Sliwa 2005

[学会発表] Femtosecond Time-Resolved Diflectance Spectroscopy of Titania and SnTTP-Incorporated Titania Nanotubes : Dynamics of Surface Charge-Carriers

Ki Seok Jeon, Joon Hee Jang, Tsuyoshi Asahi, Hiroshi Masuhara, MingJoon Yoon 2005

[学会発表] Femtosecond Laser-induced Shockwave study on morphological dynamics of single living cells

Hiroshi Masuhara, Yoishiroh Hosokawa, Takuji Tada, Tsuyoshi Asahi 2005

[雑誌論文] Cooperative Photochemical Reaction Mechanism of Femtosecond Laser-Induced Photocoloration in Spirooxazines Microcrystals Examined by Femtosecond Double Pulse Experiments

Mototsugu Suzuki, Tsuyoshi Asahi, Hiroshi Masuhara 2005

[雑誌論文] Design, Synthesis, Structural and Nonlinear Optical Properties of Photochromic Crystals : Towards Reversible Molecular Switches

Michel Sliwa, Sylvie Letard, Isabelle Malfant, Martine Nierlich, Pascal G Lacroix, Tsuyoshi Asahi, Hiroshi Masuhara, Pei Yu, Keitaro Nakatani 2005

[学会発表] Local deformation and regeneration processes of single animal cell by focused femtosecond laser beam

Y. Hosokawa, T. Tada, T. Asahi, H. Masuhara, C. Shukunami, Y. Hiraki 2005

[雑誌論文] Development of Near-Infrared 35 fs Laser Microscope and Its Application to the Detection of Three- and Four-Photon Fluorescence of Organic Microcrystals

Hirohisa Matsuda, Yousuke Fujimoto, Syoji Ito, Yutaka Nagasawa, Hiroshi Miyasaka, Tsuyoshi Asahi, Hiroshi Masuhara 2005

[雑誌論文] Single Molecule Spectroscopy of Organic Dye Nanoparticles

Andre J. Gesquiere, Takayuki Uwada, Tsuyoshi Asahi, Hiroshi Masuhara, Paul F. Barbara 2005

[雑誌論文] Development of Near-Infrared 35 fs Laser Microscope and Its Application to the Detection of Three- and Four-Photon Fluorescence of Organic Microcrystals

Hirohisa Matsuda, Yousuke Fujimoto, Syoji Ito, Yutaka Nagasawa, Hiroshi Miyasaka, Tsuyoshi Asahi, Hiroshi Masuhara 2005

[学会発表] Development of the Near-infrared Femtosecond Laser Microscope System and its Application

Hirohisa Matsuda, Syoji Ito, Yutaka Nagasawa, Hiroshi Miyasaka, Tsuyoshi Asahi, Hiroshi Masuhara 2005

[学会発表] Film Formation of Size-controlled Quinnacridone Nanoparticles by a modifield electrophoretic Deposition Method

Hyeon-Gu Jeon, Tsuyoshi Asahi, Teruki Sugiyama, Hiroshi Masuhara 2005

[雑誌論文] A biosensor in the time domain based on the diffusion coefficient measurement: intermolecular interaction of an intermediate of photoactive yellow protein

Javaid Shahbaz Khan, Yasushi Imamoto, Yoichi Yamazaki, Mikio Kataoka, Fumio Tokunaga, Masahide Terazima 2005

[学会発表] Development of the Near-infrared Femtosecond Laser Microscope System and its Application to Multiphoton Imaging

Hirohisa Matsuda, Shoji Ito, Yutaka Nagasawa, Tsuyoshi Asahi, Hiroshi Masuhara, Hiroshi Miyasaka 2005

[学会発表] Solvent effect on quinacridone nanoparticle formation by laser ablation of its bulk crystal in poor solvents

Teruki Sugiyama, Tsuyoshi Asahi, Kazuya Kawahara, Hiroshi Masuhara 2005

[学会発表] "Coherent Coupling of J-aggregate Exciton to Surface Plasmon Electronic Oscillations of Single Au Nanoparticles" (Invited)

Tsuyoshi Asahi, Ryo Toyota, Takayuki Uwada, Hiroshi Masuhara 2005

[学会発表] Film Formation of Size-controlled Quinnacridone Nanoparticles by a modifield electrophoretic Deposition Method

Hyeon-Gu Jeon, Tsuyoshi Asahi, Teruki Sugiyama, Hiroshi Masuhara 2005

[学会発表] "Femtosecond laser-induced melting of anthracene crystal studied by ultrafast microscopy" (Invited)

Tsuyoshi Asahi, Hiroshi Masuhara 2005

[学会発表] "Spectroscopic and Photochemical Studies on Single Organic Nanoparticles" (Invited)

Hiroshi Masuhara, Tsuyoshi Asahi 2005

[学会発表] Single Particle Spectroscopic Investigation on Gold Nanoparticles Coverd with Cyanine Dye J-aggregate

Takayuki Uwada, Ryo Toyota, Tsuyoshi Asahi, Hiroshi Masuhara 2005