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寺井健太

徳島大学

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リサーチマップ・
Jグローバル
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2024年11月22日更新

職名: 教授
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電子メール: terai.kenta.5m@tokushima-u.ac.jp
学歴: 2002/3: 北海道大学, 医学部, 医学科卒
学位: 博士(医学) (大阪大学) (2006年3月)
職歴・経歴: 2023/12: 徳島大学教授, 大学院医歯薬学研究部

専門分野・研究分野: ライフサイエンス

研究者総覧で最新データを確認する

2024年11月22日更新

専門分野・研究分野: ライフサイエンス
担当経験のある授業科目: 解剖学Ⅰ (学部)
解剖学Ⅰ・解剖学Ⅰ実習 (学部)
解剖学Ⅰ実習 (学部)
指導経験: 研究者総覧に該当データはありませんでした。

研究者総覧で最新データを確認する

2024年11月22日更新

専門分野・研究分野: ライフサイエンス

研究テーマ: 実験病理学, 医化学

著書

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論文

Kimiya Matsuda, Daiki Hirayama, Naoya Hino, Sota Kuno, Asako Sakaue-Sawano, Atsushi Miyawaki, Michiyuki Matsuda and Kenta Terai :
Knockout of all ErbB-family genes delineates their roles in proliferation, survival and migration.,
Journal of Cell Science, Vol.136, No.16, jcs261199, 2023.

(要約): The ErbB-family receptors play pivotal roles in the proliferation, migration and survival of epithelial cells. Because our knowledge on the ErbB-family receptors has been largely obtained by the exogenous application of their ligands, it remains unknown to what extent each of the ErbB members contributes to these outputs. We here knocked out each ErbB gene, various combinations of ErbB genes or all ErbB genes in Madin-Darby canine kidney cells to delineate the contribution of each gene. ERK1 and ERK2 (ERK1/2, also known as MAPK3 and MAPK1, respectively) activation waves during collective cell migration were mediated primarily by ErbB1 and secondarily by the ErbB2 and ErbB3 heterodimer. Either ErbB1 or the ErbB2 and ErbB3 complex was sufficient for the G1/S progression. The saturation cell density was markedly reduced in cells deficient in all ErbB proteins, but not in cells retaining only ErbB2, which cannot bind to ligands. Thus, a ligand-independent ErbB2 activity is sufficient for preventing apoptosis at high cell density. In short, systematic knockout of ErbB-family genes has delineated the roles of each ErbB receptor.
(キーワード): Animals / Dogs / Ligands / シグナル伝達 (signal transduction) / Receptor, ErbB-2 / リン酸化 (phosphorylation) / Genes, erbB / Cell Proliferation / Receptor, ErbB-3
(出版サイトへのリンク): ● Publication site (DOI): 10.1242/jcs.261199
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37519219; ● Summary page in Scopus @ Elsevier: 2-s2.0-85168428420

(DOI: 10.1242/jcs.261199, PubMed: 37519219, Elsevier: Scopus) Yoshinobu Konishi, Hiroshi Ichise, Tetsuya Watabe, Choji Oki, Shinya Tsukiji, Yoko Hamazaki, Yasuhiro Murakawa, Akifumi Takaori-Kondo, Kenta Terai and Michiyuki Matsuda :
Intravital Imaging Identifies the VEGF-TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion.,
Cancer Research, Vol.81, No.15, 4124-4132, 2021.

(要約): Prostaglandin E (PGE) promotes tumor progression through evasion of antitumor immunity. In stark contrast to cyclooxygenase-dependent production of PGE, little is known whether PGE secretion is regulated within tumor tissues. Here, we show that VEGF-dependent release of thromboxane A (TXA) triggers Ca transients in tumor cells, culminating in PGE secretion and subsequent immune evasion in the early stages of tumorigenesis. Ca transients caused cPLA2 activation and triggered the arachidonic acid cascade. Ca transients were monitored as the surrogate marker of PGE secretion. Intravital imaging of Braf mouse melanoma cells revealed that the proportion of cells exhibiting Ca transients is markedly higher than . The TXA receptor was indispensable for the Ca transients , high intratumoral PGE concentration, and evasion of antitumor immunity. Notably, treatment with a VEGF receptor antagonist and an anti-VEGF antibody rapidly suppressed Ca transients and reduced TXA and PGE concentrations in tumor tissues. These results identify the VEGF-TXA axis as a critical promoter of PGE-dependent tumor immune evasion, providing a molecular basis underlying the immunomodulatory effect of anti-VEGF therapies. SIGNIFICANCE: This study identifies the VEGF-TXA axis as a potentially targetable regulator of PGE secretion, which provides novel strategies for prevention and treatment of multiple types of malignancies.
(キーワード): Animals / Dinoprostone / Humans / Immune Evasion / Intravital Microscopy / Mice / Mice, Nude / Vascular Endothelial Growth Factor A
(出版サイトへのリンク): ● Publication site (DOI): 10.1158/0008-5472.CAN-20-4245
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34035084; ● Summary page in Scopus @ Elsevier: 2-s2.0-85111690712

(DOI: 10.1158/0008-5472.CAN-20-4245, PubMed: 34035084, Elsevier: Scopus) Ayako Imanishi, Hiroshi Ichise, Chuyun Fan, Yasuaki Nakagawa, Koichiro Kuwahara, Kenta Sumiyama, Michiyuki Matsuda and Kenta Terai :
Visualization of Spatially-Controlled Vasospasm by Sympathetic Nerve-Mediated ROCK Activation.,
The American Journal of Pathology, Vol.191, No.1, 194-203, 2021.

(要約): Contraction of vascular smooth muscle is regulated primarily by calcium concentration and secondarily by ROCK activity within the cells. In contrast to the wealth of information regarding regulation of calcium concentration, little is known about the spatiotemporal regulation of ROCK activity in live blood vessels. Here, we report ROCK activation in subcutaneous arterioles in a transgenic mouse line that expresses a genetically encoded ROCK biosensor based on the principle of F rster resonance energy transfer by two-photon excitation in vivo imaging. Rapid vasospasm was induced upon laser ablation of arterioles, concomitant with a transient increase in calcium concentration in arteriolar smooth muscles. Unlike the increase in calcium concentration, vasoconstriction and ROCK activation continued for several minutes after irradiation. Both the ROCK inhibitor, fasudil, and the ganglionic nicotinic acetylcholine receptor blocker, hexamethonium, inhibited laser-induced ROCK activation and reduced the duration of vasospasm at the segments distant from the irradiated point. These observations suggest that vasoconstriction is initially triggered by a rapid surge of cytoplasmic calcium and then maintained by sympathetic nerve-mediated ROCK activation.
(キーワード): Animals / Autonomic Nervous System / Calcium Signaling / Fluorescence Resonance Energy Transfer / Mice / Mice, Inbred C57BL / Mice, Transgenic / Muscle, Smooth, Vascular / Vasoconstriction / rho-Associated Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ajpath.2020.09.012
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33069718; ● Summary page in Scopus @ Elsevier: 2-s2.0-85097782185

(DOI: 10.1016/j.ajpath.2020.09.012, PubMed: 33069718, Elsevier: Scopus) Tomoaki Kinjo, Tetsuya Watabe, Kenju Kobachi, Kenta Terai and Michiyuki Matsuda :
Single-Cell Activation of the cAMP-Signaling Pathway in 3D Tissues with FRET-Assisted Two-Photon Activation of bPAC.,
ACS Chemical Biology, Vol.15, No.11, 2848-2853, 2020.

(要約): Bacterial photoactivated adenylyl cyclase (bPAC) has been widely used in signal transduction research. However, due to its low two-photon absorption, bPAC cannot be efficiently activated by two-photon (2P) excitation. Taking advantage of the high two-photon absorption of monomeric teal fluorescent protein 1 (mTFP1), we herein developed 2P-activatable bPAC (2pabPAC), a fusion protein consisting of bPAC and mTFP1. In 2pabPAC, the energy absorbed by mTFP1 excites bPAC by Fürster resonance energy transfer (FRET) at . 43% efficiency. The light-induced increase in cAMP was monitored by a red-shifted FRET biosensor for PKA. In 3D MDCK cells and mouse liver, PKA was activated at single-cell resolution under a 2P microscope. We found that PKA activation in a single hepatocyte caused PKA activation in neighboring cells, indicating the propagation of PKA activation. Thus, 2pabPAC will provide a versatile platform for controlling the cAMP signaling pathway and investigating cell-to-cell communication .
(キーワード): Adenylyl Cyclases / Animals / Bacteria / Biosensing Techniques / Cell Communication / Cyclic AMP / Cyclic AMP-Dependent Protein Kinases / Dogs / Enzyme Activation / Fluorescence Resonance Energy Transfer / Fluorescent Dyes / Green Fluorescent Proteins / HeLa Cells / Humans / Liver / Madin Darby Canine Kidney Cells / Mice / シグナル伝達 (signal transduction) / Single-Cell Analysis
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/acschembio.0c00333
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33074647; ● Summary page in Scopus @ Elsevier: 2-s2.0-85096048266

(DOI: 10.1021/acschembio.0c00333, PubMed: 33074647, Elsevier: Scopus) Yumi Konagaya, Kanako Takakura, Maina Sogabe, Anjali Bisaria, Chad Liu, Tobias Meyer, Atsuko Sehara-Fujisawa, Michiyuki Matsuda and Kenta Terai :
Intravital imaging reveals cell cycle-dependent myogenic cell migration during muscle regeneration.,
Cell Cycle, Vol.19, No.22, 3167-3181, 2020.

(要約): During muscle regeneration, extracellular signal-regulated kinase (ERK) promotes both proliferation and migration. However, the relationship between proliferation and migration is poorly understood in this context. To elucidate this complex relationship on a physiological level, we established an intravital imaging system for measuring ERK activity, migration speed, and cell-cycle phases in mouse muscle satellite cell-derived myogenic cells. We found that , ERK is maximally activated in myogenic cells two days after injury, and this is then followed by increases in cell number and motility. With limited effects of ERK activity on migration on an acute timescale, we hypothesized that ERK increases migration speed in the later phase by promoting cell-cycle progression. Our cell-cycle analysis further revealed that in myogenic cells, ERK activity is critical for G1/S transition, and cells migrate more rapidly in S/G2 phase 3 days after injury. Finally, migration speed of myogenic cells was suppressed after CDK1/2-but not CDK1-inhibitor treatment, demonstrating a critical role of CDK2 in myogenic cell migration. Overall, our study demonstrates that in myogenic cells, the ERK-CDK2 axis promotes not only G1/S transition but also migration, thus providing a novel mechanism for efficient muscle regeneration.
(キーワード): Animals / Cardiotoxins / 細胞周期 (cell cycle) / Cell Line / Cell Movement / Cell Proliferation / Cyclin-Dependent Kinase 2 / Epithelial Cells / Extracellular Signal-Regulated MAP Kinases / 女性 (female) / Humans / Intravital Microscopy / MAP Kinase Signaling System / Male / Mice / Mice, Transgenic / Muscle Development / Muscle Fibers, Skeletal / Muscle, Skeletal / 再生 (regeneration) / Satellite Cells, Skeletal Muscle / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/15384101.2020.1838779
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33131406; ● Summary page in Scopus @ Elsevier: 2-s2.0-85094872452

(DOI: 10.1080/15384101.2020.1838779, PubMed: 33131406, Elsevier: Scopus) Chad Liu, Yumi Konagaya, Mingyu Chung, Leighton H. Daigh, Yilin Fan, Hee Won Yang, Kenta Terai, Michiyuki Matsuda and Tobias Meyer :
Altered G1 signaling order and commitment point in cells proliferating without CDK4/6 activity.,
Nature Communications, Vol.11, No.1, 5305, 2020.

(要約): Cell-cycle entry relies on an orderly progression of signaling events. To start, cells first activate the kinase cyclin D-CDK4/6, which leads to eventual inactivation of the retinoblastoma protein Rb. Hours later, cells inactivate APC/C and cross the final commitment point. However, many cells with genetically deleted cyclin Ds, which activate and confer specificity to CDK4/6, can compensate and proliferate. Despite its importance in cancer, how this entry mechanism operates remains poorly characterized, and whether cells use this path under normal conditions remains unknown. Here, using single-cell microscopy, we demonstrate that cells with acutely inhibited CDK4/6 enter the cell cycle with a slowed and fluctuating cyclin E-CDK2 activity increase. Surprisingly, with low CDK4/6 activity, the order of APC/C and Rb inactivation is reversed in both cell lines and wild-type mice. Finally, we show that as a consequence of this signaling inversion, Rb inactivation replaces APC/C inactivation as the point of no return. Together, we elucidate the molecular steps that enable cell-cycle entry without CDK4/6 activity. Our findings not only have implications in cancer resistance, but also reveal temporal plasticity underlying the G1 regulatory circuit.
(キーワード): Animals / Cell Line / Cell Proliferation / Cyclin-Dependent Kinase 4 / Cyclin-Dependent Kinase 6 / 女性 (female) / G1 Phase / Humans / 男性 (male) / Mice / Retinoblastoma Protein / シグナル伝達 (signal transduction)
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41467-020-18966-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33082317; ● Summary page in Scopus @ Elsevier: 2-s2.0-85092927440

(DOI: 10.1038/s41467-020-18966-9, PubMed: 33082317, Elsevier: Scopus) Kenju Kobachi, Sota Kuno, Shinya Sato, Kenta Sumiyama, Michiyuki Matsuda and Kenta Terai :
Biliverdin Reductase-A Deficiency Brighten and Sensitize Biliverdin-binding Chromoproteins.,
Cell Structure and Function, Vol.45, No.2, 131-141, 2020.

(要約): Tissue absorbance, light scattering, and autofluorescence are significantly lower in the near-infrared (NIR) range than in the visible range. Because of these advantages, NIR fluorescent proteins (FPs) are in high demand for in vivo imaging. Nevertheless, application of NIR FPs such as iRFP is still limited due to their dimness in mammalian cells. In contrast to GFP and its variants, iRFP requires biliverdin (BV) as a chromophore. The dimness of iRFP is at least partly due to rapid reduction of BV by biliverdin reductase-A (BLVRA). Here, we established biliverdin reductase-a knockout (Blvra) mice to increase the intracellular BV concentration and, thereby, to enhance iRFP fluorescence intensity. As anticipated, iRFP fluorescence intensity was significantly increased in all examined tissues of Blvra mice. Similarly, the genetically encoded calcium indicator NIR-GECO1, which is engineered based on another NIR FP, mIFP, exhibited a marked increase in fluorescence intensity in mouse embryonic fibroblasts derived from Blvra mice. We expanded this approach to an NIR light-sensing optogenetic tool, the BphP1-PpsR2 system, which also requires BV as a chromophore. Again, deletion of the Blvra gene markedly enhanced the light response in HeLa cells. These results indicate that the Blvra mouse is a versatile tool for the in vivo application of NIR FPs and NIR light-sensing optogenetic tools.Key words: in vivo imaging, near-infrared fluorescent protein, biliverdin, biliverdin reductase, optogenetic tool.
(キーワード): Animals / Biliverdine / Embryo, Mammalian / Fibroblasts / Green Fluorescent Proteins / HeLa Cells / Humans / Mice / ノックアウトマウス (knockout mice) / Oxidoreductases Acting on CH-CH Group Donors
(出版サイトへのリンク): ● Publication site (DOI): 10.1247/csf.20010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32581154; ● Summary page in Scopus @ Elsevier: 2-s2.0-85089796493

(DOI: 10.1247/csf.20010, PubMed: 32581154, Elsevier: Scopus) Tetsuya Watabe, Kenta Terai, Kenta Sumiyama and Michiyuki Matsuda :
Booster, a Red-Shifted Genetically Encoded Förster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools.,
ACS Sensors, Vol.5, No.3, 719-730, 2020.

(要約): Genetically encoded Förster resonance energy transfer (FRET)-based biosensors have been developed for the visualization of signaling molecule activities. Currently, most of them are comprised of cyan and yellow fluorescent proteins (CFP and YFP), precluding the use of multiple FRET biosensors within a single cell. Moreover, the FRET biosensors based on CFP and YFP are incompatible with the optogenetic tools that operate at blue light. To overcome these problems, here, we have developed FRET biosensors with red-shifted excitation and emission wavelengths. We chose mKOκ and mKate2 as the favorable donor and acceptor pair by calculating the Förster distance. By optimizing the order of fluorescent proteins and modulatory domains of the FRET biosensors, we developed a FRET biosensor backbone named "Booster". The performance of the protein kinase A (PKA) biosensor based on the Booster backbone (Booster-PKA) was comparable to that of AKAR3EV, a previously developed FRET biosensor comprising CFP and YFP. For the proof of concept, we first showed simultaneous monitoring of activities of two protein kinases with Booster-PKA and ERK FRET biosensors based on CFP and YFP. Second, we showed monitoring of PKA activation by photoactivated adenylyl cyclase, an optogenetic generator of cyclic AMP. Finally, we presented PKA activity in living tissues of transgenic mice expressing Booster-PKA. Collectively, the results demonstrate the effectiveness and versatility of Booster biosensors as an imaging tool in vitro and in vivo.
(キーワード): Adenylyl Cyclases / Animals / Bacterial Proteins / Biosensing Techniques / Cyclic AMP-Dependent Protein Kinases / Dogs / 女性 (female) / Fluorescence Resonance Energy Transfer / Green Fluorescent Proteins / HEK293 Cells / HeLa Cells / Humans / Light / Luminescent Proteins / Madin Darby Canine Kidney Cells / 男性 (male) / Mice, Transgenic / Optogenetics / Spectrometry, Fluorescence
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/acssensors.9b01941
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32101394; ● Summary page in Scopus @ Elsevier: 2-s2.0-85082560839

(DOI: 10.1021/acssensors.9b01941, PubMed: 32101394, Elsevier: Scopus) Tomoaki Kinjo, Kenta Terai, Shoichiro Horita, Norimichi Nomura, Kenta Sumiyama, Kaori Togashi, So Iwata and Michiyuki Matsuda :
FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics.,
Nature Methods, Vol.16, No.10, 1029-1036, 2019.

(要約): Optical dimerizers have been developed to untangle signaling pathways, but they are of limited use in vivo, partly due to their inefficient activation under two-photon (2P) excitation. To overcome this problem, we developed Förster resonance energy transfer (FRET)-assisted photoactivation, or FRAPA. On 2P excitation, mTagBFP2 efficiently absorbs and transfers the energy to the chromophore of CRY2. Based on structure-guided engineering, a chimeric protein with 40% FRET efficiency was developed and named 2P-activatable CRY2, or 2paCRY2. 2paCRY2 was employed to develop a RAF1 activation system named 2paRAF. In three-dimensionally cultured cells expressing 2paRAF, extracellular signal-regulated kinase (ERK) was efficiently activated by 2P excitation at single-cell resolution. Photoactivation of ERK was also accomplished in the epidermal cells of 2paRAF-expressing mice. We further developed an mTFP1-fused LOV domain that exhibits efficient response to 2P excitation. Collectively, FRAPA will pave the way to single-cell optical control of signaling pathways in vivo.
(キーワード): Animals / Enzyme Activation / Extracellular Signal-Regulated MAP Kinases / Flavoproteins / Fluorescence Resonance Energy Transfer / Mice / Optogenetics / Photons
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41592-019-0541-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31501546; ● Summary page in Scopus @ Elsevier: 2-s2.0-85072017202

(DOI: 10.1038/s41592-019-0541-5, PubMed: 31501546, Elsevier: Scopus) Yoshinobu Konishi, Kenta Terai, Yasuhide Furuta, Hiroshi Kiyonari, Takaya Abe, Yoshihiro Ueda, Tatsuo Kinashi, Yoko Hamazaki, Akifumi Takaori-Kondo and Michiyuki Matsuda :
Live-Cell FRET Imaging Reveals a Role of Extracellular Signal-Regulated Kinase Activity Dynamics in Thymocyte Motility.,
iScience, Vol.10, 98-113, 2018.

(要約): Extracellular signal-regulated kinase (ERK) plays critical roles in T cell development in the thymus. Nevertheless, the dynamics of ERK activity and the role of ERK in regulating thymocyte motility remain largely unknown due to technical limitations. To visualize ERK activity in thymocytes, we here developed knockin reporter mice expressing a Förster/fluorescence resonance energy transfer (FRET)-based biosensor for ERK from the ROSA26 locus. Live imaging of thymocytes isolated from the reporter mice revealed that ERK regulates thymocyte motility in a subtype-specific manner. Negative correlation between ERK activity and motility was observed in CD4/CD8 double-positive thymocytes and CD8 single-positive thymocytes, but not in CD4 single-positive thymocytes. Interestingly, however, the temporal deviations of ERK activity from the average correlate with the motility of CD4 single-positive thymocytes. Thus, live-cell FRET imaging will open a window to understanding the dynamic nature and the diverse functions of ERK signaling in T cell biology.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.isci.2018.11.025
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30508722; ● Summary page in Scopus @ Elsevier: 2-s2.0-85065418713

(DOI: 10.1016/j.isci.2018.11.025, PubMed: 30508722, Elsevier: Scopus) Ayako Imanishi, Tomokazu Murata, Masaya Sato, Kazuhiro Hotta, Itaru Imayoshi, Michiyuki Matsuda and Kenta Terai :
A Novel Morphological Marker for the Analysis of Molecular Activities at the Single-cell Level.,
Cell Structure and Function, Vol.43, No.2, 129-140, 2018.

(要約): For more than a century, hematoxylin and eosin (H&E) staining has been the de facto standard for histological studies. Consequently, the legacy of histological knowledge is largely based on H&E staining. Due to the recent advent of multi-photon excitation microscopy, the observation of live tissue is increasingly being used in many research fields. Adoption of this technique has been further accelerated by the development of genetically encoded biosensors for ions and signaling molecules. However, H&E-based histology has not yet begun to fully utilize in vivo imaging due to the lack of proper morphological markers. Here, we report a genetically encoded fluorescent marker, NuCyM (Nucleus, Cytosol, and Membrane), which is designed to recapitulate H&E staining patterns in vivo. We generated a transgenic mouse line ubiquitously expressing NuCyM by using a ROSA26 bacterial artificial chromosome (BAC) clone. NuCyM evenly marked the plasma membrane, cytoplasm and nucleus in most tissues, yielding H&E staining-like images. In the NuCyM-expressing cells, cell division of a single cell was clearly observed as five basic phases during M phase by three-dimensional imaging. We next crossed NuCyM mice with transgenic mice expressing an ERK biosensor based on the principle of Förster resonance energy transfer (FRET). Using NuCyM, ERK activity in each cell could be extracted from the FRET images. To further accelerate the image analysis, we employed machine learning-based segmentation methods, and thereby automatically quantitated ERK activity in each cell. In conclusion, NuCyM is a versatile cell morphological marker that enables us to grasp histological information as with H&E staining.Key words: in vivo imaging, histology, machine learning, molecular activity.
(キーワード): Animals / Biosensing Techniques / Dogs / Fluorescence Resonance Energy Transfer / Imaging, Three-Dimensional / MAP Kinase Signaling System / 機械学習 (machine learning) / Madin Darby Canine Kidney Cells / Mice, Inbred C57BL / Mice, Transgenic / Microscopy, Fluorescence / Single-Cell Analysis
(出版サイトへのリンク): ● Publication site (DOI): 10.1247/csf.18013
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29962383; ● Summary page in Scopus @ Elsevier: 2-s2.0-85051623038

(DOI: 10.1247/csf.18013, PubMed: 29962383, Elsevier: Scopus) Naoki Komatsu, Kenta Terai, Ayako Imanishi, Yuji Kamioka, Kenta Sumiyama, Takashi Jin, Yasushi Okada, Takeharu Nagai and Michiyuki Matsuda :
A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging.,
Scientific Reports, Vol.8, No.1, 8984, 2018.

(要約): Genetically encoded biosensors based on the principle of Förster resonance energy transfer comprise two major classes: biosensors based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET). The FRET biosensors visualize signaling-molecule activity in cells or tissues with high resolution. Meanwhile, due to the low background signal, the BRET biosensors are primarily used in drug screening. Here, we report a protocol to transform intramolecular FRET biosensors to BRET-FRET hybrid biosensors called hyBRET biosensors. The hyBRET biosensors retain all properties of the prototype FRET biosensors and also work as BRET biosensors with dynamic ranges comparable to the prototype FRET biosensors. The hyBRET biosensors are compatible with optogenetics, luminescence microplate reader assays, and non-invasive whole-body imaging of xenograft and transgenic mice. This simple protocol will expand the use of FRET biosensors and enable visualization of the multiscale dynamics of cell signaling in live animals.
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-018-27174-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29895862; ● Summary page in Scopus @ Elsevier: 2-s2.0-85048748254

(DOI: 10.1038/s41598-018-27174-x, PubMed: 29895862, Elsevier: Scopus) Yumi Konagaya, Kenta Terai, Yusuke Hirao, Kanako Takakura, Masamichi Imajo, Yuji Kamioka, Norio Sasaoka, Akira Kakizuka, Kenta Sumiyama, Tomoichiro Asano and Michiyuki Matsuda :
A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs.,
Cell Reports, Vol.21, No.9, 2628-2638, 2017.

(要約): AMP-activated protein kinase (AMPK), a master regulator of cellular metabolism, is a potential target for type 2 diabetes. Although extensive in vitro studies have revealed the complex regulation of AMPK, much remains unknown about the regulation in vivo. We therefore developed transgenic mice expressing a highly sensitive fluorescence resonance energy transfer (FRET)-based biosensor for AMPK, called AMPKAR-EV. AMPKAR-EV allowed us to readily examine the role of LKB1, a canonical stimulator of AMPK, in drug-induced activation and inactivation of AMPK in vitro. In transgenic mice expressing AMPKAR-EV, the AMP analog AICAR activated AMPK in muscle. In contrast, the antidiabetic drug metformin activated AMPK in liver, highlighting the organ-specific action of AMPK stimulators. Moreover, we found that AMPK was activated primarily in fast-twitch muscle fibers after tetanic contraction and exercise. These observations suggest that the AMPKAR-EV mouse will pave a way to understanding the heterogeneous responses of AMPK among cell types in vivo.
(キーワード): AMP-Activated Protein Kinases / Animals / Biosensing Techniques / 女性 (female) / Fluorescence Resonance Energy Transfer / Liver / 男性 (male) / Mice / Muscle, Skeletal / シグナル伝達 (signal transduction)
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.celrep.2017.10.113
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29186696; ● Summary page in Scopus @ Elsevier: 2-s2.0-85035765777

(DOI: 10.1016/j.celrep.2017.10.113, PubMed: 29186696, Elsevier: Scopus) Ayumi Nagasawa-Masuda and Kenta Terai :
Yap/Taz transcriptional activity is essential for vascular regression via Ctgf expression and actin polymerization.,
PLoS ONE, Vol.12, No.4, e0174633, 2017.

(要約): Vascular regression is essential to remove redundant vessels during the formation of an efficient vascular network that can transport oxygen and nutrient to every corner of the body. However, no mechanism is known to explain how major blood vessels regress during development. Here we use the dorsal part of the caudal vein plexus (dCVP) in Zebrafish to investigate the mechanism of regression and discover a new role of Yap/Taz in vascular regression. During regression, Yap/Taz is activated by blood circulation in the endothelial cells. This leads to induction of Ctgf and actin polymerization. Interference with Yap/Taz activation decreased Ctgf production, which decreased actin polymerization and vascular regression. These results implicate a novel role of Yap/Taz in vascular regression.
(キーワード): Actins / Acyltransferases / Animals / Animals, Genetically Modified / Cell Line / Connective Tissue Growth Factor / HEK293 Cells / Human Umbilical Vein Endothelial Cells / Humans / In Situ Hybridization / Morpholinos / Neovascularization, Physiologic / Trans-Activators / Transcription Factors / Transcriptional Activation / YAP-Signaling Proteins / Zebrafish / Zebrafish Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0174633
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28369143; ● Summary page in Scopus @ Elsevier: 2-s2.0-85016644452

(DOI: 10.1371/journal.pone.0174633, PubMed: 28369143, Elsevier: Scopus) Hiroyuki Nakajima, Kimiko Yamamoto, Sobhika Agarwala, Kenta Terai, Hajime Fukui, Shigetomo Fukuhara, Koji Ando, Takahiro Miyazaki, Yasuhiro Yokota, Etienne Schmelzer, Heinz-Georg Belting, Markus Affolter, Virginie Lecaudey and Naoki Mochizuki :
Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.,
Developmental Cell, Vol.40, No.6, 523-536.e6, 2017.

(要約): Endothelial cells (ECs) line the inside of blood vessels and respond to mechanical cues generated by blood flow. Mechanical stimuli regulate the localization of YAP by reorganizing the actin cytoskeleton. Here we demonstrate blood-flow-mediated regulation of endothelial YAP in vivo. We indirectly monitored transcriptional activity of Yap1 (zebrafish YAP) and its spatiotemporal localization in living zebrafish and found that Yap1 entered the nucleus and promoted transcription in response to blood flow. In cultured human ECs, laminar shear stress induced nuclear import of YAP and its transcriptional activity in a manner independent of Hippo signaling. We uncovered a molecular mechanism by which flow induced the nuclear translocation of YAP through the regulation of filamentous actin and angiomotin. Yap1 mutant zebrafish showed a defect in vascular stability, indicating an essential role for Yap1 in blood vessels. Our data imply that endothelial Yap1 functions in response to flow to maintain blood vessels.
(キーワード): Actins / Adaptor Proteins, Signal Transducing / Animals / Blood Vessels / Cell Nucleus / Endothelial Cells / Hemorheology / Human Umbilical Vein Endothelial Cells / Humans / Intercellular Signaling Peptides and Proteins / Membrane Proteins / Perfusion / Phosphoproteins / Protein Serine-Threonine Kinases / Protein Transport / Serine-Threonine Kinase 3 / Shear Strength / シグナル伝達 (signal transduction) / Stress, Mechanical / Trans-Activators / Transcription Factors / Transcription, Genetic / Transcriptional Activation / YAP-Signaling Proteins / Zebrafish / Zebrafish Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.devcel.2017.02.019
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28350986; ● Summary page in Scopus @ Elsevier: 2-s2.0-85016450607

(DOI: 10.1016/j.devcel.2017.02.019, PubMed: 28350986, Elsevier: Scopus) Ayano Chiba, Haruko Watanabe-Takano, Kenta Terai, Hajime Fukui, Takahiro Miyazaki, Mami Uemura, Hisashi Hashimoto, Masahiko Hibi, Shigetomo Fukuhara and Naoki Mochizuki :
Osteocrin, a peptide secreted from the heart and other tissues, contributes to cranial osteogenesis and chondrogenesis in zebrafish.,
Development, Vol.144, No.2, 334-344, 2017.

(要約): The heart is an endocrine organ, as cardiomyocytes (CMs) secrete natriuretic peptide (NP) hormones. Since the discovery of NPs, no other peptide hormones that affect remote organs have been identified from the heart. We identified osteocrin (Ostn) as an osteogenesis/chondrogenesis regulatory hormone secreted from CMs in zebrafish. ostn mutant larvae exhibit impaired membranous and chondral bone formation. The impaired bones were recovered by CM-specific overexpression of OSTN. We analyzed the parasphenoid (ps) as a representative of membranous bones. In the shortened ps of ostn morphants, nuclear Yap1/Wwtr1-dependent transcription was increased, suggesting that Ostn might induce the nuclear export of Yap1/Wwtr1 in osteoblasts. Although OSTN is proposed to bind to NPR3 (clearance receptor for NPs) to enhance the binding of NPs to NPR1 or NPR2, OSTN enhanced C-type NP (CNP)-dependent nuclear export of YAP1/WWTR1 of cultured mouse osteoblasts stimulated with saturable CNP. OSTN might therefore activate unidentified receptors that augment protein kinase G signaling mediated by a CNP-NPR2 signaling axis. These data demonstrate that Ostn secreted from the heart contributes to bone formation as an endocrine hormone.
(キーワード): Animal Structures / Animals / Animals, Genetically Modified / Cells, Cultured / Chondrogenesis / Embryo, Nonmammalian / HEK293 Cells / 心臓 (heart) / Humans / Mice / Myocytes, Cardiac / Organogenesis / Osteogenesis / Peptide Hormones / Skull / Transcription Factors / Zebrafish / Zebrafish Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1242/dev.143354
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27993976; ● Summary page in Scopus @ Elsevier: 2-s2.0-85010040214

(DOI: 10.1242/dev.143354, PubMed: 27993976, Elsevier: Scopus) Chunjie Li, Ayako Imanishi, Naoki Komatsu, Kenta Terai, Mutsuki Amano, Kozo Kaibuchi and Michiyuki Matsuda :
A FRET Biosensor for ROCK Based on a Consensus Substrate Sequence Identified by KISS Technology.,
Cell Structure and Function, Vol.42, No.1, 1-13, 2017.

(要約): Genetically-encoded biosensors based on Förster/fluorescence resonance energy transfer (FRET) are versatile tools for studying the spatio-temporal regulation of signaling molecules within not only the cells but also tissues. Perhaps the hardest task in the development of a FRET biosensor for protein kinases is to identify the kinase-specific substrate peptide to be used in the FRET biosensor. To solve this problem, we took advantage of kinase-interacting substrate screening (KISS) technology, which deduces a consensus substrate sequence for the protein kinase of interest. Here, we show that a consensus substrate sequence for ROCK identified by KISS yielded a FRET biosensor for ROCK, named Eevee-ROCK, with high sensitivity and specificity. By treating HeLa cells with inhibitors or siRNAs against ROCK, we show that a substantial part of the basal FRET signal of Eevee-ROCK was derived from the activities of ROCK1 and ROCK2. Eevee-ROCK readily detected ROCK activation by epidermal growth factor, lysophosphatidic acid, and serum. When cells stably-expressing Eevee-ROCK were time-lapse imaged for three days, ROCK activity was found to increase after the completion of cytokinesis, concomitant with the spreading of cells. Eevee-ROCK also revealed a gradual increase in ROCK activity during apoptosis. Thus, Eevee-ROCK, which was developed from a substrate sequence predicted by the KISS technology, will pave the way to a better understanding of the function of ROCK in a physiological context.
(キーワード): Amino Acid Sequence / Biosensing Techniques / Blotting, Western / Fluorescence Resonance Energy Transfer / 遺伝子発現 (gene expression) / HeLa Cells / Humans / Microscopy, Fluorescence / リン酸化 (phosphorylation) / Plasmids / Protein Kinase Inhibitors / RNA Interference / RNA, Small Interfering / Substrate Specificity / Time-Lapse Imaging / rho-Associated Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1247/csf.16016
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27885213; ● Summary page in Scopus @ Elsevier: 2-s2.0-85011579520

(DOI: 10.1247/csf.16016, PubMed: 27885213, Elsevier: Scopus) Ayumi Nagasawa-Masuda and Kenta Terai :
ERK activation in endothelial cells is a novel marker during neovasculogenesis.,
Genes to Cells, Vol.21, No.11, 1164-1175, 2016.

(要約): Vasculogenesis is essential during early development to construct networks transporting oxygen, blood and nutrients. Tip and stalk cells are specialized endothelial cells involved in novel vessel formation because of their behavior such as sprouting as a leading cell and following tip cell. However, the spatiotemporal details determining the emergence of these cells are unknown. Here, we first show that the ERK activity in endothelial cells represents the precursor of tip and stalk cells for vasculogenesis in zebrafish. We identified that tip and stalk cells for intersegmental vessel (ISV) formation were already specialized in the dorsal aorta (DA) before sprouting. Furthermore, similar specialization was observed in tip cells during parachordal vessel (PAV) formation in lymphangiogenesis. We also identified that the ERK activity was required for specialized cells to emerge from existing blood vessels. Our data show that the ERK activity is a novel marker for determining the emergence of cells in both angiogenesis and lymphangiogenesis.
(キーワード): Animals / Aorta / Endothelial Cells / Enzyme Activation / Extracellular Signal-Regulated MAP Kinases / Neovascularization, Physiologic / Veins / Zebrafish / Zebrafish Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/gtc.12438
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27696620; ● Summary page in Scopus @ Elsevier: 2-s2.0-84990228909

(DOI: 10.1111/gtc.12438, PubMed: 27696620, Elsevier: Scopus) Mami Uemura, Ayumi Nagasawa and Kenta Terai :
Yap/Taz transcriptional activity in endothelial cells promotes intramembranous ossification via the BMP pathway.,
Scientific Reports, Vol.6, 27473, 2016.

(要約): Osteogenesis is categorized into two groups based on developmental histology, intramembranous and endochondral ossification. The role of blood vessels during endochondral ossification is well known, while their role in intramembranous ossification, especially the intertissue pathway, is poorly understood. Here, we demonstrate endothelial Yap/Taz is a novel regulator of intramembranous ossification in zebrafish. Appropriate blood flow is required for Yap/Taz transcriptional activation in endothelial cells and intramembranous ossification. Additionally, Yap/Taz transcriptional activity in endothelial cells specifically promotes intramembranous ossification. BMP expression by Yap/Taz transactivation in endothelial cells is also identified as a bridging factor between blood vessels and intramembranous ossification. Furthermore, the expression of Runx2 in pre-osteoblast cells is a downstream target of Yap/Taz transcriptional activity in endothelial cells. Our results provide novel insight into the relationship between blood flow and ossification by demonstrating intertissue regulation.
(キーワード): Animals / Bone Morphogenetic Proteins / Core Binding Factor Alpha 1 Subunit / Osteogenesis / Transcription Factors / Transcription, Genetic / Transcriptional Activation / Zebrafish
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/srep27473
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27273480; ● Summary page in Scopus @ Elsevier: 2-s2.0-84976350990

(DOI: 10.1038/srep27473, PubMed: 27273480, Elsevier: Scopus) Takeru Kashiwada, Shigetomo Fukuhara, Kenta Terai, Toru Tanaka, Yuki Wakayama, Koji Ando, Hiroyuki Nakajima, Hajime Fukui, Shinya Yuge, Yoshinobu Saito, Akihiko Gemma and Naoki Mochizuki :
β-Catenin-dependent transcription is central to Bmp-mediated formation of venous vessels.,
Development, Vol.142, No.3, 497-509, 2015.

(要約): β-catenin regulates the transcription of genes involved in diverse biological processes, including embryogenesis, tissue homeostasis and regeneration. Endothelial cell (EC)-specific gene-targeting analyses in mice have revealed that β-catenin is required for vascular development. However, the precise function of β-catenin-mediated gene regulation in vascular development is not well understood, since β-catenin regulates not only gene expression but also the formation of cell-cell junctions. To address this question, we have developed a novel transgenic zebrafish line that allows the visualization of β-catenin transcriptional activity specifically in ECs and discovered that β-catenin-dependent transcription is central to the bone morphogenetic protein (Bmp)-mediated formation of venous vessels. During caudal vein (CV) formation, Bmp induces the expression of aggf1, a putative causative gene for Klippel-Trenaunay syndrome, which is characterized by venous malformation and hypertrophy of bones and soft tissues. Subsequently, Aggf1 potentiates β-catenin transcriptional activity by acting as a transcriptional co-factor, suggesting that Bmp evokes β-catenin-mediated gene expression through Aggf1 expression. Bmp-mediated activation of β-catenin induces the expression of Nr2f2 (also known as Coup-TFII), a member of the nuclear receptor superfamily, to promote the differentiation of venous ECs, thereby contributing to CV formation. Furthermore, β-catenin stimulated by Bmp promotes the survival of venous ECs, but not that of arterial ECs. Collectively, these results indicate that Bmp-induced activation of β-catenin through Aggf1 regulates CV development by promoting the Nr2f2-dependent differentiation of venous ECs and their survival. This study demonstrates, for the first time, a crucial role of β-catenin-mediated gene expression in the development of venous vessels.
(キーワード): Angiogenic Proteins / Animals / Animals, Genetically Modified / Bone Morphogenetic Proteins / COUP Transcription Factor II / DNA, Complementary / Endothelial Cells / Gene Expression Regulation, Developmental / HEK293 Cells / Humans / In Situ Nick-End Labeling / Luciferases / Luminescent Proteins / Microscopy, Fluorescence / Morpholinos / Real-Time Polymerase Chain Reaction / Reverse Transcriptase Polymerase Chain Reaction / Sequence Analysis, RNA / Veins / Zebrafish / Zebrafish Proteins / beta Catenin / Red Fluorescent Protein
(出版サイトへのリンク): ● Publication site (DOI): 10.1242/dev.115576
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25564648; ● Summary page in Scopus @ Elsevier: 2-s2.0-84921522118

(DOI: 10.1242/dev.115576, PubMed: 25564648, Elsevier: Scopus) Hajime Fukui, Kenta Terai, Hiroyuki Nakajima, Ayano Chiba, Shigetomo Fukuhara and Naoki Mochizuki :
S1P-Yap1 signaling regulates endoderm formation required for cardiac precursor cell migration in zebrafish.,
Developmental Cell, Vol.31, No.1, 128-136, 2014.

(要約): To form the primary heart tube in zebrafish, bilateral cardiac precursor cells (CPCs) migrate toward the midline beneath the endoderm. Mutants lacking endoderm and fish with defective sphingosine 1-phosphate (S1P) signaling exhibit cardia bifida. Endoderm defects lead to the lack of foothold for the CPCs, whereas the cause of cardia bifida in S1P signaling mutants remains unclear. Here we show that S1P signaling regulates CPC migration through Yes-associated protein 1 (Yap1)-dependent endoderm survival. Cardia bifida seen in spns2 (S1P transporter) morphants and s1pr2 (S1P receptor-2) morphants could be rescued by endodermal expression of nuclear localized form of yap1. yap1 morphants had decreased expression of the Yap1/Tead target connective tissue growth factor a (Ctgfa) and consequently increased endodermal cell apoptosis. Consistently, ctgfa morphants showed defects of the endodermal sheet and cardia bifida. Collectively, we show that S1pr2/Yap1-regulated ctgfa expression is essential for the proper endoderm formation required for CPC migration.
(キーワード): Animals / アポトーシス (apoptosis) / Carrier Proteins / Cell Movement / Connective Tissue Growth Factor / Endoderm / Lysophospholipids / Membrane Proteins / Muscle Development / Myoblasts, Cardiac / Receptors, Lysosphingolipid / シグナル伝達 (signal transduction) / Sphingosine / Trans-Activators / YAP-Signaling Proteins / Zebrafish / Zebrafish Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.devcel.2014.08.014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25313964; ● Summary page in Scopus @ Elsevier: 2-s2.0-84907963306

(DOI: 10.1016/j.devcel.2014.08.014, PubMed: 25313964, Elsevier: Scopus) Kanae Echizen, Mitsutoshi Nakada, Tomoatsu Hayashi, Hemragul Sabit, Takuya Furuta, Miyuki Nakai, Ryo Koyama-Nasu, Yukiko Nishimura, Kenzui Taniue, Yasuyuki Morishita, Shinji Hirano, Kenta Terai, Tomoki Todo, Yasushi Ino, Akitake Mukasa, Shunsaku Takayanagi, Ryohei Ohtani, Nobuhito Saito and Tetsu Akiyama :
PCDH10 is required for the tumorigenicity of glioblastoma cells.,
Biochemical and Biophysical Research Communications, Vol.444, No.1, 13-18, 2014.

(要約): Protocadherin10 (PCDH10)/OL-protocadherin is a cadherin-related transmembrane protein that has multiple roles in the brain, including facilitating specific cell-cell connections, cell migration and axon guidance. It has recently been reported that PCDH10 functions as a tumor suppressor and that its overexpression inhibits proliferation or invasion of multiple tumor cells. However, the function of PCDH10 in glioblastoma cells has not been elucidated. In contrast to previous reports on other tumors, we show here that suppression of the expression of PCDH10 by RNA interference (RNAi) induces the growth arrest and apoptosis of glioblastoma cells in vitro. Furthermore, we demonstrate that knockdown of PCDH10 inhibits the growth of glioblastoma cells xenografted into immunocompromised mice. These results suggest that PCDH10 is required for the proliferation and tumorigenicity of glioblastoma cells. We speculate that PCDH10 may be a promising target for the therapy of glioblastoma.
(キーワード): Animals / アポトーシス (apoptosis) / Brain Neoplasms / Cadherins / Cell Line, Tumor / Cell Proliferation / Gene Knockdown Techniques / Glioblastoma / Heterografts / Humans / Mice / Mice, Nude / Neoplasm Invasiveness / Oncogene Proteins / Protocadherins / RNA Interference
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2013.12.138
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24406169; ● Summary page in Scopus @ Elsevier: 2-s2.0-84893831765

(DOI: 10.1016/j.bbrc.2013.12.138, PubMed: 24406169, Elsevier: Scopus) Kenta Terai, Etsuko Shibata, Tarek Abbas and Anindya Dutta :
Degradation of p12 subunit by CRL4Cdt2 E3 ligase inhibits fork progression after DNA damage.,
The Journal of Biological Chemistry, Vol.288, No.42, 30509-30514, 2013.

(要約): After acute DNA damage, the cell arrests S-phase progression by inhibiting origin initiation and fork progression to repair damaged DNA. The intra-S-phase checkpoint kinase Chk1 phosphorylates Cdc25A to target the latter for degradation by CRL1(β-TrCP) and so inhibit origin firing. The mechanism for inhibiting fork progression, however, has not been identified. Here, we show that degradation of p12, the fourth subunit of DNA polymerase δ, is critical for inhibiting fork progression. CRL4(Cdt2) is an E3 ligase that ubiquitinates and degrades p12 after UV treatment. Cells expressing a stable form of p12 exhibit UV-resistant DNA synthesis. DNA fiber assay and alkaline-sucrose gradient assay demonstrate that the impairment of fork progression after DNA damage requires p12 degradation. These results suggest that ubiquitination of p12 through CRL4(Cdt2) and subsequent degradation form one mechanism by which a cell responds to DNA damage to inhibit fork progression.
(キーワード): DNA (DNA) / DNA Damage / DNA Polymerase III / Enzyme Stability / HeLa Cells / Humans / Nuclear Proteins / Protein Subunits / Proteolysis / Ubiquitin-Protein Ligases / Ubiquitination / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.C113.505586
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24022480; ● Summary page in Scopus @ Elsevier: 2-s2.0-84886919244

(DOI: 10.1074/jbc.C113.505586, PubMed: 24022480, Elsevier: Scopus) Kenta Terai and Naoki Mochizuki :
Heat shock proteins regulates cardiomyocytes via Toll-like receptors.,
Circulation Journal, Vol.75, No.10, 2322-2323, 2011.

(キーワード): Animals / HSP70 Heat-Shock Proteins / Myocardial Contraction / Myocytes, Cardiac / Toll-Like Receptor 2
(出版サイトへのリンク): ● Publication site (DOI): 10.1253/circj.cj-11-0647
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21817812; ● Summary page in Scopus @ Elsevier: 2-s2.0-80053061770

(DOI: 10.1253/circj.cj-11-0647, PubMed: 21817812, Elsevier: Scopus) Rie Matsunaga-Udagawa, Yoshihisa Fujita, Sayaka Yoshiki, Kenta Terai, Yuji Kamioka, Etsuko Kiyokawa, Katsuyuki Yugi, Kazuhiro Aoki and Michiyuki Matsuda :
The scaffold protein Shoc2/SUR-8 accelerates the interaction of Ras and Raf.,
The Journal of Biological Chemistry, Vol.285, No.10, 7818-7826, 2010.

(要約): Shoc2/SUR-8 positively regulates Ras/ERK MAP kinase signaling by serving as a scaffold for Ras and Raf. Here, we examined the role of Shoc2 in the spatio-temporal regulation of Ras by using a fluorescence resonance energy transfer (FRET)-based biosensor, together with computational modeling. In epidermal growth factor-stimulated HeLa cells, RNA-mediated Shoc2 knockdown reduced the phosphorylation of MEK and ERK with half-maximal inhibition, but not the activation of Ras. For the live monitoring of Ras binding to Raf, we utilized a FRET biosensor wherein Ras and the Ras-binding domain of Raf were connected tandemly and sandwiched with acceptor and donor fluorescent proteins for the FRET measurement. With this biosensor, we found that Shoc2 was required for the rapid interaction of Ras with Raf upon epidermal growth factor stimulation. To decipher the molecular mechanisms underlying the kinetics, we developed two computational models that might account for the action of Shoc2 in the Ras-ERK signaling. One of these models, the Shoc2 accelerator model, provided a reasonable explanation of the experimental observations. In this Shoc2 accelerator model, Shoc2 accelerated both the association and dissociation of Ras-Raf interaction. We propose that Shoc2 regulates the spatio-temporal patterns of the Ras-ERK signaling pathway primarily by accelerating the Ras-Raf interaction.
(キーワード): Animals / Biosensing Techniques / Computer Simulation / Enzyme Activation / Epidermal Growth Factor / Extracellular Signal-Regulated MAP Kinases / Fluorescence Resonance Energy Transfer / HeLa Cells / Humans / Intracellular Signaling Peptides and Proteins / Mitogen-Activated Protein Kinase Kinases / リン酸化 (phosphorylation) / Protein Binding / RNA Interference / シグナル伝達 (signal transduction) / raf Kinases / ras Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M109.053975
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20051520; ● Summary page in Scopus @ Elsevier: 2-s2.0-77951219799

(DOI: 10.1074/jbc.M109.053975, PubMed: 20051520, Elsevier: Scopus) Kenta Terai, Tarek Abbas, Amir A. Jazaeri and Anindya Dutta :
CRL4(Cdt2) E3 ubiquitin ligase monoubiquitinates PCNA to promote translesion DNA synthesis.,
Molecular Cell, Vol.37, No.1, 143-149, 2010.

(要約): Monoubiquitination of proliferating cell nuclear antigen (PCNA) is a critical posttranslational modification essential for DNA repair by translesion DNA synthesis (TLS). The Rad18 E3 ubiquitin ligase cooperates with the E2 Rad6 to monoubiquitinate PCNA in response to DNA damage. How PCNA is monoubiquitinated in unperturbed cells and whether this plays a role in the repair of DNA associated with replication is not known. We show that the CRL4(Cdt2) E3 ubiquitin ligase complex promotes PCNA monoubiqutination in proliferating cells in the absence of external DNA damage independent of Rad18. PCNA monoubiquitination via CRL4(Cdt2) is constitutively antagonized by the action of the ubiquitin-specific protease 1 (USP1). In vitro, CRL4(Cdt2) monoubiquitinates PCNA at Lys164, the same residue that is monoubiquitinated by Rad18. Significantly, CRL4(Cdt2) is required for TLS in nondamaged cells via a mechanism that is dependent on PCNA monoubiquitination. We propose that CRL4(Cdt2) regulates PCNA-dependent TLS associated with stresses accompanying DNA replication.
(キーワード): Cell Line / DNA Damage / DNA Replication / DNA-Binding Proteins / HeLa Cells / Humans / Nuclear Proteins / Proliferating Cell Nuclear Antigen / Ubiquitin-Protein Ligases / Ubiquitination
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.molcel.2009.12.018
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20129063; ● Summary page in Scopus @ Elsevier: 2-s2.0-73649125005

(DOI: 10.1016/j.molcel.2009.12.018, PubMed: 20129063, Elsevier: Scopus) Tarek Abbas, Uma Sivaprasad, Kenta Terai, Virginia Amador, Michele Pagano and Anindya Dutta :
PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex.,
Genes & Development, Vol.22, No.18, 2496-2506, 2008.

(要約): The DNA polymerase delta processivity factor Proliferating Cell Nuclear Antigen (PCNA) promotes the DNA damage-induced degradation of the replication initiation factor Cdt1 via the CRL4(Cdt2) E3 ubiquitin ligase complex. Here we demonstrate that PCNA promotes the ubiquitylation and degradation of the CDK inhibitor p21 in cells irradiated with low dose of ultraviolet (UV) by a similar mechanism. Human cells that are depleted of Cul4, DDB1 (damage-specific DNA-binding protein-1), or the DCAF Cdt2, are deficient in the UV-induced ubiquitylation and degradation of p21. Depletion of mammalian cells of PCNA by siRNA, or mutations in p21 that abrogate PCNA binding, prevent UV-induced p21 ubiquitylation and degradation, indicating that physical binding with PCNA is necessary for the efficient ubiquitylation of p21 via the CRL4(Cdt2) ubiquitin ligase. Cdt2 functions as the substrate recruiting factor for p21 to the rest of the CRL4 ubiquitin ligase complex. The CRL4(Cdt2) E3 ubiquitin ligase ubiquitylates p21 both in vivo and in vitro, and its activity is dependent on the interaction of p21 with PCNA. Finally, we show that the CRL4(Cdt2) and the SCF(Skp2) ubiquitin ligases are redundant with each other in promoting the degradation of p21 during an unperturbed S phase of the cell cycle.
(キーワード): Base Sequence / Cyclin-Dependent Kinase Inhibitor p21 / Humans / Hydrolysis / Proliferating Cell Nuclear Antigen / Proteasome Endopeptidase Complex / RNA Interference / RNA, Small Interfering / S Phase / S-Phase Kinase-Associated Proteins / Ubiquitin-Protein Ligases / Ubiquitination / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.1101/gad.1676108
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18794347; ● Search Scopus @ Elsevier (PMID): 18794347; ● Search Scopus @ Elsevier (DOI): 10.1101/gad.1676108

(DOI: 10.1101/gad.1676108, PubMed: 18794347) Kenta Terai and Michiyuki Matsuda :
The amino-terminal B-Raf-specific region mediates calcium-dependent homo- and hetero-dimerization of Raf.,
The EMBO Journal, Vol.25, No.15, 3556-3564, 2006.

(要約): B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK). B-Raf differs from the other Raf isoforms in that it has a long amino-terminal region. By the use of probes based on the principle of fluorescence resonance energy transfer, we found that this amino-terminal B-Raf-specific region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. HeLa cells expressing B-Raf, but not c-Raf, or a B-Raf mutant lacking the B-Raf-specific region, showed enhanced MEK phosphorylation upon stimulation with a calcium agonist. Furthermore, increases in the intracellular calcium concentration were found to be necessary for dimerization and sufficient for the plasma membrane translocation of B-Raf. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Thus, we propose that the hitherto unidentified function of the B-Raf amino-terminal region is to mediate calcium-dependent activation of B-Raf and the following MEK activation, which may occur in the absence of Ras activation.
(キーワード): カルシウム (calcium) / Calcium Signaling / Cell Membrane / Dimerization / Fluorescence Resonance Energy Transfer / Green Fluorescent Proteins / HeLa Cells / Humans / Mitogen-Activated Protein Kinase Kinases / Models, Biological / リン酸化 (phosphorylation) / Proto-Oncogene Proteins B-raf / Proto-Oncogene Proteins c-raf / Threonine / ras Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/sj.emboj.7601241
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16858395; ● Summary page in Scopus @ Elsevier: 2-s2.0-33747615527

(DOI: 10.1038/sj.emboj.7601241, PubMed: 16858395, Elsevier: Scopus) Noriko Takegahara, Hyota Takamatsu, Toshihiko Toyofuku, Tohru Tsujimura, Tatsusada Okuno, Kazunori Yukawa, Masayuki Mizui, Midori Yamamoto, Durbaka V. R. Prasad, Kazuhiro Suzuki, Masaru Ishii, Kenta Terai, Masayuki Moriya, Yuji Nakatsuji, Saburo Sakoda, Shintaro Sato, Shizuo Akira, Kiyoshi Takeda, Masanori Inui, Toshiyuki Takai, Masahito Ikawa, Masaru Okabe, Atsushi Kumanogoh and Hitoshi Kikutani :
Plexin-A1 and its interaction with DAP12 in immune responses and bone homeostasis.,
Nature Cell Biology, Vol.8, No.6, 615-622, 2006.

(要約): Semaphorins and their receptors have diverse functions in axon guidance, organogenesis, vascularization and/or angiogenesis, oncogenesis and regulation of immune responses. The primary receptors for semaphorins are members of the plexin family. In particular, plexin-A1, together with ligand-binding neuropilins, transduces repulsive axon guidance signals for soluble class III semaphorins, whereas plexin-A1 has multiple functions in chick cardiogenesis as a receptor for the transmembrane semaphorin, Sema6D, independent of neuropilins. Additionally, plexin-A1 has been implicated in dendritic cell function in the immune system. However, the role of plexin-A1 in vivo, and the mechanisms underlying its pleiotropic functions, remain unclear. Here, we generated plexin-A1-deficient (plexin-A1(-/-)) mice and identified its important roles, not only in immune responses, but also in bone homeostasis. Furthermore, we show that plexin-A1 associates with the triggering receptor expressed on myeloid cells-2 (Trem-2), linking semaphorin-signalling to the immuno-receptor tyrosine-based activation motif (ITAM)-bearing adaptor protein, DAP12. These findings reveal an unexpected role for plexin-A1 and present a novel signalling mechanism for exerting the pleiotropic functions of semaphorins.
(キーワード): Adaptor Proteins, Signal Transducing / Animals / Bone and Bones / 生体恒常性 (homeostasis) / 免疫 (immunity) / Membrane Glycoproteins / Mice / ノックアウトマウス (knockout mice) / Nerve Tissue Proteins / Receptors, Cell Surface / Receptors, Immunologic / シグナル伝達 (signal transduction)
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/ncb1416
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16715077; ● Summary page in Scopus @ Elsevier: 2-s2.0-33744815942

(DOI: 10.1038/ncb1416, PubMed: 16715077, Elsevier: Scopus) Aki Fujioka, Kenta Terai, Reina E. Itoh, Kazuhiro Aoki, Takeshi Nakamura, Shinya Kuroda, Eisuke Nishida and Michiyuki Matsuda :
Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes.,
The Journal of Biological Chemistry, Vol.281, No.13, 8917-8926, 2006.

(要約): To comprehend the Ras/ERK MAPK cascade, which comprises Ras, Raf, MEK, and ERK, several kinetic simulation models have been developed. However, a large number of parameters that are essential for the development of these models are still missing and need to be set arbitrarily. Here, we aimed at collecting these missing parameters using fluorescent probes. First, the levels of the signaling molecules were quantitated. Second, to monitor both the activation and nuclear translocation of ERK, we developed probes based on the principle of fluorescence resonance energy transfer. Third, the dissociation constants of Ras.Raf, Raf.MEK, and MEK.ERK complexes were estimated using a fluorescent tag that can be highlighted very rapidly. Finally, the same fluorescent tag was used to measure the nucleocytoplasmic shuttling rates of ERK and MEK. Using these parameters, we developed a kinetic simulation model consisting of the minimum essential members of the Ras/ERK MAPK cascade. This simple model reproduced essential features of the observed activation and nuclear translocation of ERK. In this model, the concentration of Raf significantly affected the levels of phospho-MEK and phospho-ERK upon stimulation. This prediction was confirmed experimentally by decreasing the level of Raf using the small interfering RNA technique. This observation verified the usefulness of the parameters collected in this study.
(キーワード): Animals / Biological Transport, Active / COS Cells / Cell Culture Techniques / Cell Nucleus / Chlorocebus aethiops / Clone Cells / Computer Simulation / Cytoplasm / Enzyme Activation / Extracellular Signal-Regulated MAP Kinases / Flow Cytometry / Fluorescence Resonance Energy Transfer / Fluorescent Dyes / HeLa Cells / Humans / Kinetics / Mitogen-Activated Protein Kinase Kinases / Mitogen-Activated Protein Kinases / Models, Biological / Molecular Probe Techniques / Molecular Probes / リン酸化 (phosphorylation) / Proto-Oncogene Proteins c-raf / RNA, Small Interfering / ras Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M509344200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16418172; ● Summary page in Scopus @ Elsevier: 2-s2.0-33646852421

(DOI: 10.1074/jbc.M509344200, PubMed: 16418172, Elsevier: Scopus) Kenta Terai and Michiyuki Matsuda :
Ras binding opens c-Raf to expose the docking site for mitogen-activated protein kinase kinase.,
EMBO Reports, Vol.6, No.3, 251-255, 2005.

(要約): A key signalling molecule, c-Raf, is situated downstream from Ras and upstream from the mitogen-activated protein kinase kinase (MEK). We studied the mechanism underlying the signal transduction from Ras to MEK by using probes based on the principle of fluorescence resonance energy transfer. In agreement with previous models, it was found that c-Raf adopted two conformations: open active and closed inactive. Ras binding induced the c-Raf transition from closed to open conformation, which enabled c-Raf to bind to MEK. In the presence of a cytosolic Ras mutant, c-Raf bound to, but failed to phosphorylate, MEK in the cytoplasm. In contrast, the cytosolic Ras mutant significantly enhanced MEK phosphorylation by a membrane-targeted c-Raf. These results demonstrated the essential role of Ras-induced conformational change in MEK activation by c-Raf.
(キーワード): Amino Acid Substitution / Animals / Binding Sites / Cell Line / Chlorocebus aethiops / Humans / Mitogen-Activated Protein Kinase Kinases / Mutation / Protein Binding / Protein Conformation / Proto-Oncogene Proteins c-raf / ras Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/sj.embor.7400349
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15711535; ● Summary page in Scopus @ Elsevier: 2-s2.0-16844385887

(DOI: 10.1038/sj.embor.7400349, PubMed: 15711535, Elsevier: Scopus) Taro Kawai, Shintaro Sato, Ken J. Ishii, Cevayir Coban, Hiroaki Hemmi, Masahiro Yamamoto, Kenta Terai, Michiyuki Matsuda, Jun-ichiro Inoue, Satoshi Uematsu, Osamu Takeuchi and Shizuo Akira :
Interferon-alpha induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6.,
Nature Immunology, Vol.5, No.10, 1061-1068, 2004.

(要約): Toll-like receptors (TLRs) are involved in the recognition of microbial pathogens. A subset of TLRs, TLR7, TLR8 and TLR9, induces antiviral responses by producing interferon-alpha (IFN-alpha). Production of IFN-alpha is dependent on the Toll-interleukin-1 receptor domain-containing adaptor MyD88. Here we show that MyD88 formed a complex with the transcription factor IRF7 but not with IRF3. The death domain of MyD88 interacted with an inhibitory domain of IRF7, and this interaction resulted in activation of the IFN-alpha-dependent promoters. Furthermore, the adaptor molecule TRAF6 also bound and activated IRF7. Ubiquitin ligase activity of TRAF6 was required for IRF7 activation. These results indicate that TLR-mediated IFN-alpha induction requires the formation of a complex consisting of MyD88, TRAF6 and IRF7 as well as TRAF6-dependent ubiquitination.
(キーワード): Adaptor Proteins, Signal Transducing / Animals / Antigens, Differentiation / DNA-Binding Proteins / Humans / Interferon Regulatory Factor-7 / Interferon-alpha / Membrane Glycoproteins / Mice / Mice, Inbred C57BL / Myeloid Differentiation Factor 88 / Proteins / Receptors, Cell Surface / Receptors, Immunologic / TNF Receptor-Associated Factor 6 / Toll-Like Receptor 7 / Toll-Like Receptor 8 / Toll-Like Receptor 9 / Toll-Like Receptors / Ubiquitin
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/ni1118
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15361868; ● Summary page in Scopus @ Elsevier: 2-s2.0-5444274514

(DOI: 10.1038/ni1118, PubMed: 15361868, Elsevier: Scopus) Kazuo Kurokawa, Akiyuki Takaya, Kenta Terai, Aki Fujioka and Michiyuki Matsuda :
Visualizing the Signal Transduction Pathways in Living Cells with GFP-Based FRET Probes,
Acta Histochemica et Cytochemica, Vol.37, No.6, 347-355, 2004.

(キーワード): fluorescence resonance energy transfer / green fluorescence protein / 生物工学 (biotechnology) / fluorescence microscopy
(出版サイトへのリンク): ● Publication site (DOI): 10.1267/ahc.37.347
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679839383552; ● Summary page in Scopus @ Elsevier: 2-s2.0-15744365890

(DOI: 10.1267/ahc.37.347, CiNii: 1390282679839383552, Elsevier: Scopus)

MISC

Tetsuya Watabe, Shinya Yamahira, Kanako Takakura, Dean Thumkeo, Shuh Narumiya, Michiyuki Matsuda and Kenta Terai :
Calcium transients trigger switch-like discharge of prostaglandin E₂ in an extracellular signal-regulated kinase-dependent manner,
eLife, 2024.

(出版サイトへのリンク): ● Publication site (DOI): 10.7554/eLife.86727.2
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.7554/eLife.86727.2

(DOI: 10.7554/eLife.86727.2) Tetsuya Watabe, Shinya Yamahira, Michiyuki Matsuda and Kenta Terai :
Visual quantification of prostaglandin E₂ discharge from a single cell.,
Cell Structure and Function, Vol.48, No.2, 241-249, 2023.

(要約): Calcium transients drive cells to discharge prostaglandin E (PGE). We visualized PGE-induced protein kinase A (PKA) activation and quantitated PGE secreted from a single cell by combining fluorescence microscopy and a simulation model. For this purpose, we first prepared PGE-producer cells that express either an optogenetic or a chemogenetic calcium channel stimulator: OptoSTIM1 or Gq-DREADD, respectively. Second, we prepared reporter cells expressing the Gs-coupled PGE reporter EP2 and the PKA biosensor Booster-PKA, which is based on the principle of Förster resonance energy transfer (FRET). Upon the stimulation-induced triggering of calcium transients, a single producer cell discharges PGE to stimulate PKA in the surrounding reporter cells. Due to the flow of the medium, the PKA-activated area exhibited a comet-like smear when HeLa cells were used. In contrast, radial PKA activation was observed when confluent MDCK cells were used, indicating that PGE diffusion was restricted to the basolateral space. By fitting the radius of the PKA-activated area to a simulation model based on simple diffusion, we estimated that a single HeLa cell secretes 0.25 fmol PGE upon a single calcium transient to activate PKA in more than 1000 neighboring cells. This model also predicts that the PGE discharge rate is comparable to the diffusion rate. Thus, our method quantitatively envisions that a single calcium transient affects more than 1000 neighboring cells via PGE.Key words: prostaglandin E, imaging, intercellular communication, biosensor, quantification.
(キーワード): Animals / Dogs / Humans / HeLa Cells / Dinoprostone / Madin Darby Canine Kidney Cells / Fluorescence Resonance Energy Transfer
(出版サイトへのリンク): ● Publication site (DOI): 10.1247/csf.23047
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37813623; ● Summary page in Scopus @ Elsevier: 2-s2.0-85178951335

(DOI: 10.1247/csf.23047, PubMed: 37813623, Elsevier: Scopus) Yoshinobu Konishi and Kenta Terai :
In vivo imaging of inflammatory response in cancer research.,
Inflammation and Regeneration, Vol.43, No.1, 10, 2023.

(要約): Inflammation can contribute to the development and progression of cancer. The inflammatory responses in the tumor microenvironment are shaped by complex sequences of dynamic intercellular cross-talks among diverse types of cells, and recapitulation of these dynamic events in vitro has yet to be achieved. Today, intravital microscopy with two-photon excitation microscopes (2P-IVM) is the mainstay technique for observing intercellular cross-talks in situ, unraveling cellular and molecular mechanisms in the context of their spatiotemporal dynamics. In this review, we summarize the current state of 2P-IVM with fluorescent indicators of signal transduction to reveal the cross-talks between cancer cells and surrounding cells including both immune and non-immune cells. We also discuss the potential application of red-shifted indicators along with optogenetic tools to 2P-IVM. In an era of single-cell transcriptomics and data-driven research, 2P-IVM will remain a key advantage in delivering the missing spatiotemporal context in the field of cancer research.
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s41232-023-00261-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36750856; ● Search Scopus @ Elsevier (PMID): 36750856; ● Search Scopus @ Elsevier (DOI): 10.1186/s41232-023-00261-x

(DOI: 10.1186/s41232-023-00261-x, PubMed: 36750856) Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki, Kenta Terai, Sachiko Komazawa-Sakon, Takao Seki, Yoshifumi Yamaguchi, Tetuo Mikami, Kimi Araki, Masaki Ohmuraya, Michiyuki Matsuda and Hiroyasu Nakano :
Generation of transgenic mice expressing a FRET biosensor, SMART, that responds to necroptosis.,
Communications Biology, Vol.5, No.1, 1331, 2022.

(要約): Necroptosis is a regulated form of cell death involved in various pathological conditions, including ischemic reperfusion injuries, virus infections, and drug-induced tissue injuries. However, it is not fully understood when and where necroptosis occurs in vivo. We previously generated a Forster resonance energy transfer (FRET) biosensor, termed SMART (the sensor for MLKL activation by RIPK3 based on FRET), which monitors conformational changes of MLKL along with progression of necroptosis in human and murine cell lines in vitro. Here, we generate transgenic (Tg) mice that express the SMART biosensor in various tissues. The FRET ratio is increased in necroptosis, but not apoptosis or pyroptosis, in primary cells. Moreover, the FRET signals are elevated in renal tubular cells of cisplatin-treated SMART Tg mice compared to untreated SMART Tg mice. Together, SMART Tg mice may provide a valuable tool for monitoring necroptosis in different types of cells in vitro and in vivo.
(キーワード): Humans / Mice / Animals / Necroptosis / Fluorescence Resonance Energy Transfer / Mice, Transgenic / Protein Kinases / Biosensing Techniques
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s42003-022-04300-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36471162; ● Summary page in Scopus @ Elsevier: 2-s2.0-85143415101

(DOI: 10.1038/s42003-022-04300-0, PubMed: 36471162, Elsevier: Scopus) Naoya Hino, Kimiya Matsuda, Yuya Jikko, Gembu Maryu, Katsuya Sakai, Ryu Imamura, Shinya Tsukiji, Kazuhiro Aoki, Kenta Terai, Tsuyoshi Hirashima, Xavier Trepat and Michiyuki Matsuda :
A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration.,
Developmental Cell, Vol.57, No.19, 2290-2304.e7, 2022.

(要約): Upon the initiation of collective cell migration, the cells at the free edge are specified as leader cells; however, the mechanism underlying the leader cell specification remains elusive. Here, we show that lamellipodial extension after the release from mechanical confinement causes sustained extracellular signal-regulated kinase (ERK) activation and underlies the leader cell specification. Live-imaging of Madin-Darby canine kidney (MDCK) cells and mouse epidermis through the use of Förster resonance energy transfer (FRET)-based biosensors showed that leader cells exhibit sustained ERK activation in a hepatocyte growth factor (HGF)-dependent manner. Meanwhile, follower cells exhibit oscillatory ERK activation waves in an epidermal growth factor (EGF) signaling-dependent manner. Lamellipodial extension at the free edge increases the cellular sensitivity to HGF. The HGF-dependent ERK activation, in turn, promotes lamellipodial extension, thereby forming a positive feedback loop between cell extension and ERK activation and specifying the cells at the free edge as the leader cells. Our findings show that the integration of physical and biochemical cues underlies the leader cell specification during collective cell migration.
(キーワード): Animals / Cell Movement / Dogs / Epidermal Growth Factor / Extracellular Signal-Regulated MAP Kinases / フィードバック (feedback) / Hepatocyte Growth Factor / Mice
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.devcel.2022.09.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36174555; ● Summary page in Scopus @ Elsevier: 2-s2.0-85139295167

(DOI: 10.1016/j.devcel.2022.09.003, PubMed: 36174555, Elsevier: Scopus) Shin Murai, Kanako Takakura, Kenta Sumiyama, Kenta Moriwaki, Kenta Terai, Sachiko Komazawa-Sakon, Yoshifumi Yamaguchi, Tetuo Mikami, Kimi Araki, Masaki Ohmuraya, Michiyuki Matsuda and Hiroyasu Nakano :
A FRET biosensor, SMART, monitors necroptosis in renal tubular epithelial cells in a cisplatin-induced kidney injury model,
Bio Rxiv, 2022.

(出版サイトへのリンク): ● Publication site (DOI): 10.1101/2022.06.18.496655
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1101/2022.06.18.496655

(DOI: 10.1101/2022.06.18.496655) Hiroshi Ichise, Shoko Tsukamoto, Tsuyoshi Hirashima, Yoshinobu Konishi, Choji Oki, Shinya Tsukiji, Satoshi Iwano, Atsushi Miyawaki, Kenta Sumiyama, Kenta Terai and Michiyuki Matsuda :
Functional visualization of NK cell-mediated killing of metastatic single tumor cells,
eLife, Vol.11, No.e76269, 2022.

(要約): Natural killer (NK) cells lyse invading tumor cells to limit metastatic growth in the lung, but how some cancers evade this host protective mechanism to establish a growing lesion is unknown. Here, we have combined ultra-sensitive bioluminescence imaging with intravital two-photon microscopy involving genetically encoded biosensors to examine this question. NK cells eliminated disseminated tumor cells from the lung within 24 hr of arrival, but not thereafter. Intravital dynamic imaging revealed that 50% of NK-tumor cell encounters lead to tumor cell death in the first 4 hr after tumor cell arrival, but after 24 hr of arrival, nearly 100% of the interactions result in the survival of the tumor cell. During this 24-hr period, the probability of ERK activation in NK cells upon encountering the tumor cells was decreased from 68% to 8%, which correlated with the loss of the activating ligand CD155/PVR/Necl5 from the tumor cell surface. Thus, by quantitatively visualizing, the NK-tumor cell interaction at the early stage of metastasis, we have revealed the crucial parameters of NK cell immune surveillance in the lung.
(キーワード): Animals / Biosensing Techniques / Cell Communication / Cell Line, Tumor / 女性 (female) / Immunologic Surveillance / Intravital Microscopy / Killer Cells, Natural / Luminescent Proteins / 男性 (male) / Mice / Mice, Inbred BALB C / Mice, Inbred C57BL / Neoplasm Metastasis / Neoplastic Cells, Circulating
(出版サイトへのリンク): ● Publication site (DOI): 10.7554/eLife.76269
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35113018; ● Summary page in Scopus @ Elsevier: 2-s2.0-85124635936

(DOI: 10.7554/eLife.76269, PubMed: 35113018, Elsevier: Scopus) Shuhao Lin, Daiki Hirayama, Gembu Maryu, Kimiya Matsuda, Naoya Hino, Eriko Deguchi, Kazuhiro Aoki, Ryo Iwamoto, Kenta Terai and Michiyuki Matsuda :
Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.,
Life Science Alliance, Vol.5, No.1, e202101206, 2022.

(要約): Epidermal growth factor receptor (EGFR) plays a pivotal role in collective cell migration by mediating cell-to-cell propagation of extracellular signal-regulated kinase (ERK) activation. Here, we aimed to determine which EGFR ligands mediate the ERK activation waves. We found that epidermal growth factor ()-deficient cells exhibited lower basal ERK activity than the cells deficient in heparin-binding EGF (), transforming growth factor alpha () or epiregulin (), but all cell lines deficient in a single EGFR ligand retained the ERK activation waves. Surprisingly, ERK activation waves were markedly suppressed, albeit incompletely, only when all four EGFR ligands were knocked out. Re-expression of the EGFR ligands revealed that all but HBEGF could restore the ERK activation waves. Aiming at complete elimination of the ERK activation waves, we further attempted to knockout , a ligand for ErbB3 and ErbB4, and found that -deficiency induced growth arrest in the absence of all four EGFR ligand genes. Collectively, these results showed that EGFR ligands exhibit remarkable redundancy in the propagation of ERK activation waves during collective cell migration.
(キーワード): Animals / CDC2 Protein Kinase / Cell Line / Cell Movement / Epidermal Growth Factor / ErbB Receptors / Extracellular Signal-Regulated MAP Kinases / 遺伝子発現 (gene expression) / Gene Knockdown Techniques / Humans / Ligands / Mutation / Protein Binding / RNA, Messenger / Single-Cell Analysis
(出版サイトへのリンク): ● Publication site (DOI): 10.26508/lsa.202101206
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34667080; ● Summary page in Scopus @ Elsevier: 2-s2.0-85118676805

(DOI: 10.26508/lsa.202101206, PubMed: 34667080, Elsevier: Scopus) Taisei Tanaka, Yoshinobu Konishi, Hiroshi Ichise, Shinya Tsukiji, Michiyuki Matsuda and Kenta Terai :
A Dual Promoter System to Monitor IFN-γ Signaling in vivo at Single-cell Resolution.,
Cell Structure and Function, Vol.46, No.2, 103-111, 2021.

(要約): IFN-γ secreted from immune cells exerts pleiotropic effects on tumor cells, including induction of immune checkpoint and antigen presentation, growth inhibition, and apoptosis induction. We combined a dual promoter system with an IFN-γ signaling responsive promoter to generate a reporter named the interferon sensing probe (ISP), which quantitates the response to IFN-γ by means of fluorescence and bioluminescence. The integration site effect of the transgene is compensated for by the PGK promoter-driven expression of a fluorescent protein. Among five potential IFN-γ-responsive elements, we found that the interferon γ-activated sequence (GAS) exhibited the best performance. When ISP-GAS was introduced into four cell lines and subjected to IFN-γ stimulation, dose-dependency was observed with an EC ranging from 0.2 to 0.9 ng/mL, indicating that ISP-GAS can be generally used as a sensitive biosensor of IFN-γ response. In a syngeneic transplantation model, the ISP-GAS-expressing cancer cells exhibited bioluminescence and fluorescence signals in an IFN-γ receptor-dependent manner. Thus, ISP-GAS could be used to quantitatively monitor the IFN-γ response both in vitro and in vivo.Key words: in vivo imaging, tumor microenvironment, interferon-gamma, dual promoter system.
(キーワード): Interferon-gamma / Promoter Regions, Genetic / RNA, Messenger / シグナル伝達 (signal transduction) / Transcription, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1247/csf.21052
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34744115; ● Summary page in Scopus @ Elsevier: 2-s2.0-85122903713

(DOI: 10.1247/csf.21052, PubMed: 34744115, Elsevier: Scopus) Jiazhou He, Masamichi Yamamoto, Kenta Sumiyama, Yumi Konagaya, Kenta Terai, Michiyuki Matsuda and Shinya Sato :
Two-photon AMPK and ATP imaging reveals the bias between rods and cones in glycolysis utility.,
The FASEB journal, Vol.35, No.9, e21880, 2021.

(要約): In vertebrates, retinal rod and cone photoreceptor cells rely significantly on glycolysis. Lactate released from photoreceptor cells fuels neighboring retinal pigment epithelium cells and Müller glial cells through oxidative phosphorylation. To understand this highly heterogeneous metabolic environment around photoreceptor cells, single-cell analysis is needed. Here, we visualized cellular AMP-activated protein kinase (AMPK) activity and ATP levels in the retina by two-photon microscopy. Transgenic mice expressing a hyBRET-AMPK biosensor were used for measuring the AMPK activity. GO-ATeam2 transgenic mice were used for measuring the ATP level. Temporal metabolic responses were successfully detected in the live retinal explants upon drug perfusion. A glycolysis inhibitor, 2-deoxy-d-glucose (2-DG), activated AMPK and reduced ATP. These effects were clearly stronger in rods than in cones. Notably, rod AMPK and ATP started to recover at 30 min from the onset of 2-DG perfusion. Consistent with these findings, ex vivo electroretinogram recordings showed a transient slowdown in rod dim flash responses during a 60-min 2-DG perfusion, whereas cone responses were not affected. Based on these results, we propose that cones surrounded by highly glycolytic rods become less dependent on glycolysis, and rods also become less dependent on glycolysis within 60 min upon the glycolysis inhibition.
(キーワード): AMP-Activated Protein Kinases / アデノシン三リン酸 (adenosine triphosphate) / Animals / Ependymoglial Cells / Glycolysis / Light / Mice / Mice, Inbred C57BL / 酸化的リン酸化 (oxidative phosphorylation) / Photons / 網膜 (retina) / Retinal Cone Photoreceptor Cells / Retinal Rod Photoreceptor Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1096/fj.202101121R
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34449091; ● Summary page in Scopus @ Elsevier: 2-s2.0-85113475706

(DOI: 10.1096/fj.202101121R, PubMed: 34449091, Elsevier: Scopus) Ichise Hiroshi, Tsukamoto Shoko, Hirashima Tsuyoshi, Konishi Yoshinobu, Oki Choji, Tsukiji Shinya, Iwano Satoshi, Miyawaki Atsushi, Sumiyama Kenta, Kenta Terai and Matsuda Michiyuki :
Metastatic Single Tumor Cells Evade NK Cell-mediated Killing by Thrombin-mediated Loss of the Activating Ligand CD155/PVR/Necl-5,
bioRxiv, 2021.

(要約): <jats:title>ABSTRACT</jats:title><jats:p>Natural killer (NK) cells lyse invading tumor cells to limit metastatic growth in the lung, but how some cancers evade this host protective mechanism to establish a growing lesion is not known. Here we have combined ultra-sensitive bioluminescence whole body imaging with intravital two-photon microscopy involving genetically-encoded biosensors to examine this question. NK cells eliminated disseminated tumor cells from the lung within 24 hrs of arrival, but not thereafter. Intravital dynamic imaging revealed that a disseminated tumor cell in a pulmonary capillary interacts with an NK cell every 2 hrs on average. In the first 4 hrs after tumor cell arrival, 50% of such encounters lead to tumor cell death but after 24 hrs of arrival, nearly 100% of the interactions result in the survival of the tumor cell. This evasion of NK cell surveillance is mediated by thrombin-dependent loss of cell surface CD155/PVR/Necl-5, a ligand for the NK cell activating receptor DNAM-1. This loss prevents the NK cell signaling needed for effective killing of tumor targets. By quantitatively visualizing the evasion of NK cell surveillance, we have uncovered a molecular mechanism for cancer evasion and provided an explanation for the anti-metastatic effect of anticoagulants.</jats:p><jats:sec><jats:title>SUMMARY</jats:title><jats:p>Intravital functional two-photon microscopy reveals that metastatic tumor cells lodged in pulmonary capillaries acquire resistance to patrolling NK cells. Protease-mediated loss of the activating ligand CD155/PVR/Necl-5 on tumor cells prevents NK cells from ERK activation and tumor cell killing.</jats:p></jats:sec>
(出版サイトへのリンク): ● Publication site (DOI): 10.1101/2021.01.15.426784
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1360009142755795072; ● Search Scopus @ Elsevier (DOI): 10.1101/2021.01.15.426784

(DOI: 10.1101/2021.01.15.426784, CiNii: 1360009142755795072) Michiyuki Matsuda and Kenta Terai :
Experimental pathology by intravital microscopy and genetically encoded fluorescent biosensors.,
Pathology International, Vol.70, No.7, 379-390, 2020.

(要約): The invention of two-photon excitation microscopes widens the potential application of intravital microscopy (IVM) to the broad field of experimental pathology. Moreover, the recent development of fluorescent protein-based, genetically encoded biosensors provides an ideal tool to visualize the cell function in live animals. We start from a brief review of IVM with two-photon excitation microscopes and genetically encoded biosensors based on the principle of Förster resonance energy transfer (FRET). Then, we describe how IVM using biosensors has revealed the pathogenesis of several disease models.
(キーワード): Animals / Biosensing Techniques / Disease Models, Animal / Fluorescence Resonance Energy Transfer / Intravital Microscopy / Microscopy, Fluorescence / Pathology
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/pin.12925
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32270554; ● Summary page in Scopus @ Elsevier: 2-s2.0-85083065712

(DOI: 10.1111/pin.12925, PubMed: 32270554, Elsevier: Scopus) Kenta Terai, Ayako Imanishi, Chunjie Li and Michiyuki Matsuda :
Two Decades of Genetically Encoded Biosensors Based on Förster Resonance Energy Transfer.,
Cell Structure and Function, Vol.44, No.2, 153-169, 2019.

(要約): Two decades have passed since the development of the first calcium indicator based on the green fluorescent protein (GFP) and the principle of Förster resonance energy transfer (FRET). During this period, researchers have advanced many novel ideas for the improvement of such genetically encoded FRET biosensors, which have allowed them to expand their targets from small molecules to signaling proteins and physicochemical properties. Although the merits of "genetically encoded" FRET biosensors became clear once various cell lines were established and several transgenic organisms were generated, the road to these developments was not necessarily a smooth one. Moreover, even today the development of new FRET biosensors remains a very labor-intensive, trial-and-error process. Therefore, at this junction, it may be worthwhile to summarize the progress of the FRET biosensor and discuss the future direction of its development and application.Key words: FRET, biosensor, fluorescent protein.
(キーワード): Animals / Biosensing Techniques / Fluorescence Resonance Energy Transfer / Green Fluorescent Proteins / Humans / Organisms, Genetically Modified
(出版サイトへのリンク): ● Publication site (DOI): 10.1247/csf.18035
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30905922; ● Summary page in Scopus @ Elsevier: 2-s2.0-85074486284

(DOI: 10.1247/csf.18035, PubMed: 30905922, Elsevier: Scopus)

総説・解説

寺井健太, 渡部哲也 :
長波長蛍光タンパク質を用いたFRETバイオセンサーの開発=FRETバイオセンサーと光遺伝学ツールの併用を可能に=,
光アライアンス, Vol.33, No.8, 23-26, 2022年8月. 寺井健太, 松田道行 :
バイオイメージング極秘ファイルフェルスター共鳴エネルギー移動を利用した分子活性バイオセンサー,
Optronics : 光技術コーディネートジャーナル, Vol.480, 111-114, 2021年12月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1521136281241397760

(CiNii: 1521136281241397760) 寺井健太, 松田道行 :
次世代病理技術講座第11回生体イメージングに基づく実験病理学,
病理と臨床, Vol.38, No.2, 173-177, 2020年2月. 今西彩子, 寺井健太 :
病理形態学の新技術細胞の個性を紐解く蛍光生体イメージングの進展,
病理と臨床, Vol.37, No.7, 603-608, 2019年7月. 小長谷有美, 寺井健太 :
マウス体内におけるAMPキナーゼ活性の可視化,
医学のあゆみ, Vol.269, No.12, 897-900, 2019年6月. 寺井健太, 松田道行 :
タンパク質・核酸の分子修飾 II.細胞質/オルガネラでの分子修飾細胞内シグナルリン酸化(セリオ/スレオニン),
生体の科学, Vol.69, No.5, 426-427, 2018年10月. 小長谷有美, 寺井健太, 松田道行 :
FRETバイオセンサーを用いたin vitroおよびin vivo imaging,
臨床免疫·アレルギー科, Vol.69, No.4, 380-386, 2018年4月.

(キーワード): Forster resonance energy transfer (FRET) / fluorescence resonance energy transfer / シグナル伝達 (signal transduction) / fluorescent protein
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520854805307413248

(CiNii: 1520854805307413248) 寺井健太 :
細胞多様性解明に資する光技術-見て,動かす II.見えなかったものを視るリン酸化酵素とGタンパク質のFRETバイオセンサー,
生体の科学, Vol.68, No.5, 450-451, 2017年10月.

(出版サイトへのリンク): ● Publication site (DOI): 10.11477/mf.2425200689
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282752360405248; ● Search Scopus @ Elsevier (DOI): 10.11477/mf.2425200689

(DOI: 10.11477/mf.2425200689, CiNii: 1390282752360405248) 寺井健太, 松田道行 :
FRETイメージングを用いたシグナル伝達の可視化 : 分子活性と細胞機能を顕微鏡で観る技術 (第5土曜特集生命現象を観る : 革新的な構造生命科学が観せてくれる世界) -- (FRET(蛍光共鳴エネルギー移動)),
医学のあゆみ, Vol.262, No.5, 559-565, 2017年7月.

(キーワード): フェルスター共鳴エネルギー移動 / 蛍光共鳴エネルギー移動 / シグナル伝達 (signal transduction) / 蛍光タンパク質
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520010379922635904

(CiNii: 1520010379922635904) 寺井健太, 松田道行 :
FRETを用いた蛋白間相互作用解析,
分子呼吸器病, Vol.9, No.1, 69-71, 2005年1月.

講演・発表

日野直也, 松田樹生也, 真流玄武, 酒井克也, 今村龍, 青木一洋, 寺井健太, 平島剛志, 松本邦夫, 松田道行 :
集団遊走における細胞の機械的拘束依存的な増殖因子シグナル活性動態変化による先導細胞の運命決定,
日本細胞生物学会大会(Web), 2021年. 石井衛, 寺井健太, 松田道行 :
Split蛍光タンパク質を用いた蛍光ラベル技術の創生,
日本細胞生物学会大会(Web), 2021年. 小鉢健樹, 隅山健太, 青木一洋, 松田道行, 寺井健太 :
赤色光/近赤外光による光遺伝学操作に向けたPhycocyanobilin生合成マウスの作出,
日本細胞生物学会大会(Web), 2021年. 出口英梨子, Shuhao LIN, 岩本亮, 寺井健太, 松田道行 :
MDCK細胞に発現するEGFRリガンドの分子特徴的な細胞外動態,
日本細胞生物学会大会(Web), 2021年. 塚本祥子, 一瀬大志, 寺井健太, 松田道行 :
肺転移がん細胞におけるNecl-5の欠損は免疫回避の一因である,
日本細胞生物学会大会(Web), 2021年. 塚本祥子, 一瀬大志, 寺井健太, 松田道行 :
イメージングを用いた肺転移防御における免疫逃避機構の解明,
日本癌学会学術総会抄録集(Web), 2021年. 村井晋, 隅山健太, 森脇健太, 高倉加奈子, 山口良文, 駒澤幸子, 寺井健太, 三浦正幸, 松田道行, 中野裕康 :
SMART Tgマウス由来細胞を用いたネクロプトーシスのライブセルイメージング,
日本Cell Death学会学術集会プログラム抄録集, 2021年. 松田道行, 寺井健太 :
受容体型チロシンキナーゼ/Ras/MAPキナーゼ経路の可視化,
日本癌学会学術総会抄録集(Web), 2020年. 寺井健太, 小西義延, 松田道行 :
癌細胞中のGq蛋白共役型受容体による免疫回避機構,
日本癌学会学術総会抄録集(Web), 2020年. Shuhao LIN, 平山大記, 日野直也, 真流玄武, 岩本亮, 青木一洋, 寺井健太, 松田道行 :
MDCK細胞におけるERK活性化ウエーブの伝播と集合的細胞移動におけるEGFリガンドの冗長な役割,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 日野直也, 真流玄武, 青木一洋, 寺井健太, 平島剛志, 松田道行 :
集団細胞遊走における細胞層の自由端依存的なHGF感受性上昇による先導細胞の運命決定,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 松田樹生也, 真流玄武, 平山大記, 日野直也, 青木一洋, 寺井健太, 松田道行 :
細胞の集団遊走におけるErbBファミリーの機能,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 松田道行, 一瀬大志, 小西義延, 寺井健太 :
腫瘍免疫のライブイメージング,
日本癌治療学会学術集会(Web), 2020年. 出口英梨子, 岩本亮, 寺井健太, 松田道行 :
HB-EGFの細胞間シグナル伝達における分布と生理的意義,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 范楚芸, 今西彩子, 松田道行, 寺井健太 :
ライブイメージングによるマウスクモ膜下出血時の分子活性,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 塚本祥子, 一瀬大志, 寺井健太, 松田道行 :
肺におけるがん細胞のNK細胞回避機構,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 渡部哲也, 寺井健太, 松田道行 :
プロスタグランジンE2はプロテインキナーゼAの同心円状活性化を引き起こす,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 小鉢健樹, 隅山健太, 松田道行, 寺井健太 :
ビリベルジン還元酵素欠損マウスによる近赤外イメージングおよび光遺伝学,
日本分子生物学会年会プログラム・要旨集(Web), 2020年. 金城智章, 寺井健太, 堀田彰一朗, 野村紀通, 隅山健太, 岩田想, 松田道行 :
フェルスター共鳴エネルギー移動の原理に基づく二光子励起用光遺伝学ツールの開発,
日本細胞生物学会大会(Web), 2019年. 一瀬大志, 寺井健太, 松田道行 :
肺毛細血管におけるNK細胞による転移腫瘍排除過程の可視化,
日本癌学会学術総会抄録集(Web), 2019年. 渡部哲也, 松田道行, 寺井健太 :
長波長蛍光タンパク質を用いたFRETバイオセンサーの開発,
日本分子生物学会年会プログラム・要旨集(Web), 2019年. 松月大輔, 堀田一弘, 平松侑樹, 松田道行, 寺井健太, 今西彩子 :
クラス別学習による細胞画像セグメンテーション,
日本医用画像工学会大会予稿集(CD-ROM), 2018年. 今西彩子, 佐藤雅也, 寺井健太, 隅山健太, 堀田一弘, 松田道行 :
ライブイメージングに資する4次元ヒストロジーの創生,
日本細胞生物学会大会(Web), 2017年. 千葉彩乃, 高野晴子, 宮崎敬大, 寺井健太, 望月直樹 :
心臓に発現する分泌性ペプチドOsteocrinはゼブラフィッシュの骨形成・軟骨形成に寄与する,
日本細胞生物学会大会(Web), 2017年. 千葉彩乃, 福井一, 寺井健太, 福原茂朋, 望月直樹 :
心臓再生を目指した心筋細胞分裂誘導因子の探索,
日本血管生物医学会学術集会プログラム・抄録集, 2013年. 柏田建, 寺井健太, 福原茂朋, 弦間昭彦, 望月直樹 :
血管内皮特異的なβ-catenin依存的転写調節の蛍光生体イメージング,
日本血管生物医学会学術集会プログラム・抄録集, 2013年. 福井一, 寺井健太, 福原茂朋, 望月直樹 :
S1P-Yapシグナルは内胚葉における生存シグナル発現調節を介して心筋前駆細胞移動を制御する,
日本分子生物学会年会プログラム・要旨集(Web), 2013年. 藤岡亜紀, 寺井健太, 伊藤玲奈, 青木一洋, 中村岳史, 黒田真也, 西田栄介, 松田道行 :
Ras/ERK MAPKカスケードのダイナミクス,
日本分子生物学会年会講演要旨集, 2005年. 寺井健太, 松田道行 :
FRETを用いたc-Raf-1の構造変化解析,
日本癌学会総会記事, 2004年. 寺井健太, 松田道行 :
FRETを利用したRafの制御機序の時空間的解析,
日本癌学会総会記事, 2003年.

研究会・報告書

寺井健太, 松田道行 :
【生体イメージングの最前線-絶え間ない技術革新と生命医科学の新展開】技術・機器の進歩マウス生体内イメージングに向けたツール開発,
医学のあゆみ, Vol.286, No.5, 324-328, 2023年7月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520859945345371776

(CiNii: 1520859945345371776) 寺井健太, 松田道行 :
【蛍光タンパク質センサー】赤色蛍光タンパクでのFRETプローブ開発,
細胞, Vol.55, No.5, 288-291, 2023年4月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520859370509031040

(CiNii: 1520859370509031040) 寺井健太 :
プロスタグランジンE2分泌動態の可視化,
日本病理学会会誌, Vol.112, No.1, 269, 2023年3月. 金城智章, 寺井健太 :
Foerster共鳴エネルギー移動の原理に基づく二光子励起光遺伝学操作法の開発,
生体の科学, Vol.71, No.4, 364-369, 2020年8月. 寺井健太, 松田道行 :
蛍光生体イメージングの最前線,
細胞, Vol.50, No.3, 144-145, 2018年3月. 福井一, 寺井健太, 福原茂朋, 望月直樹 :
S1P-Yapシグナルは内胚葉における生存シグナル発現調節を介して心筋前駆細胞移動を制御する,
血管, Vol.37, No.1, 44, 2014年1月. 寺井健太, 松田道行 :
FRETを用いたc-Raf-1の構造変化解析,
日本癌学会総会記事, Vol.63rd, 142, 2004年8月.

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2024年11月22日更新

専門分野・研究分野: ライフサイエンス
所属学会・所属協会: 社団法人日本解剖学会
日本細胞生物学会
社団法人日本病理学会
日本癌学会
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Ｊグローバル

Jグローバル最終確認日: 2024/11/16 01:09
氏名（漢字）: 寺井健太
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氏名（英字）: Terai Kenta
所属機関: 徳島大学

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researchmap最終確認日: 2024/11/17 01:20
氏名（漢字）: 寺井健太
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2024年11月16日更新

研究者番号: 20616073

所属（現在）: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 教授

所属（過去の研究課題 情報に基づく）*注記: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(医学域), 教授
2020/4/1 – 2023/4/1 : 京都大学, 医学研究科, 准教授
2016/4/1 – 2020/4/1 : 京都大学, 生命科学研究科, 准教授
2014/4/1 : 東京大学, 分子細胞生物学研究所, 特任助教
2012/4/1 – 2014/4/1 : 東京大学, 分子細胞生物学研究所, 助教

審査区分/研究分野

研究代表者

生物系 / 医歯薬学 / 基礎医学 / 医化学一般
生物系 / 医歯薬学 / 内科系臨床医学 / 循環器内科学
小区分49030:実験病理学関連

研究代表者以外

小区分49030:実験病理学関連
学術変革領域研究区分(Ⅱ)
小区分48020:生理学関連
学術変革領域研究区分(Ⅲ)

キーワード

研究代表者

ゼブラフィッシュ / 血管新生 / 次世代シークエンサー / ベータカテニン / 血管形成 / Hippo pathway / 膜性骨化 / fluid shear stress / 骨分化 / 動脈硬化 / 血管内皮 / イメージング / 循環器・高血圧 / AMPK / 光遺伝学 / 生体イメージング / 二光子顕微鏡 / Imaging / 代謝 / intravital imaging / 深部観察技術 / FRET / 腫瘍免疫排除 / プロスタグランジンE2 / 蛍光生体イメージング / 腫瘍微小環境 / 細胞間情報伝達

研究代表者以外

ネクロプトーシス / １分子イメージング / 全反射顕微鏡 / DAMPs / FRET / SMART / シスプラチン / in vivoイメージング / トランスジェニックマウス / HMGB1 / 1分子イメージング / イメージング / 一分子イメージング / 多光子顕微鏡 / アポトーシス / MLKL / cFLIPL / MIB2 / 人工細胞 / バイオセンサー / バイオマーカー / 情報伝達 / シグナル増幅 / 血管透過性 / 血流 / 急性呼吸窮迫症候群 / 低分子量Gタンパク質Rap1 / 腫瘍血管 / 上皮細胞増殖因子 / FRETバイオセンサー / ERKマップキナーゼ / 細胞集団運 / グリア / 近赤外蛍光タンパク質 / ERK MAPキナーゼ / 細胞間情報伝達 / ERK / マップキナーゼ / 蛍光タンパク質 / 蛍光イメージング / 細胞死 / 細胞膜障害 / １細胞イメージング / 1細胞イメージング

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寺井 健太

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研究代表者

研究代表者以外

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寺井健太