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山村正臣

徳島大学

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リサーチマップ・
Jグローバル
KAKEN
注目研究

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2024年12月23日更新

職名: 准教授
電話: 研究者総覧に該当データはありませんでした。
電子メール: yamamura.masaomi@tokushima-u.ac.jp
学歴: 2004/3: 近畿大学農学部農芸化学科卒業
2006/3: 京都大学大学院農学研究科(修士課程)応用生命科学専攻修了
2011/5: 京都大学大学院農学研究科(博士後期過程)応用生命科学専攻修了
学位: 博士(農学) (京都大学) (2011年5月)
職歴・経歴: 2010/4: 京都大学生存圏研究所特定研究員
2015/4: 京都大学生存圏研究所特任助教
2021/11: 徳島大学准教授, 大学院社会産業理工学研究部

専門分野・研究分野: 森林植物代謝学

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2024年12月23日更新

専門分野・研究分野: 森林植物代謝学
担当経験のある授業科目: 森林代謝学 (学部)
森林代謝科学特論 (大学院)
森林生物学特論 (大学院)
森林科学 (学部)
生物生産システム実習A (学部)
生物生産システム実習B (学部)
生物生産システム実習Ⅰ (学部)
生物生産システム概論 (学部)
生物生産フィールド実習 (学部)
生物生産科学概論 (学部)
生物生産科学特別演習 (大学院)
生物生産科学特別研究 (大学院)
生物資源学研究 (大学院)
生物資源産業学C (学部)
指導経験: 3人 (学士)

研究者総覧で最新データを確認する

2024年12月23日更新

専門分野・研究分野: 森林植物代謝学

研究テーマ: (心材形成機構解明に向けた基礎研究, 針葉樹心材ノルリグナン生合成機構の解明, 植物成分を利用した木材の耐腐朽性の向上, リグナン生合成機構の解明)

著書

Toshiaki Umezawa, Kenji Umemura, Masaru Kobayashi, Takuji Miyamoto, Rie Takata, Yuri Takeda-Kimura and Masaomi Yamamura :
Sustainable Production and Utilization of Grass Biomass in Deteriorated Grasslands in Indonesia,
Dec. 2023.

(出版サイトへのリンク): ● Publication site (DOI): 10.4324/9781003325154-7
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85178623338

(DOI: 10.4324/9781003325154-7, Elsevier: Scopus) 山村正臣, 梅澤俊明 :
リグニンの迅速評価システム,
株式会社シーエムシー出版, 東京, 2020年3月. 山村正臣 :
3.5.6 チオアシドリシス法のミクロ化(分担執筆),
弘前大学出版会, 弘前, 2016年2月. 山村正臣 :
3.5.2 ニトロベンゼン酸化分解法のミクロ化(分担執筆),
弘前大学出版会, 2016年2月.

(キーワード): 植物細胞壁実験法

論文

Keisuke Kobayashi, Masaomi Yamamura, Bunzo Mikami, Akira Shiraishi, Masato Kumatani, Honoo Satake, Ono Eiichiro and Toshiaki Umezawa :
Anthriscus sylvestris Deoxypodophyllotoxin Synthase Involved in the Podophyllotoxin Biosynthesis,
Plant & Cell Physiology, Vol.64, No.12, 1436-1448, 2023.

(要約): Tetrahydrofuran ring formation from dibenzylbutyrolactone lignans is a key step in the biosynthesis of aryltetralin lignans including deoxypodophyllotoxin and podophyllotoxin. Previously, Fe(II)- and 2-oxoglutarate-dependent dioxygenase (2-ODD) from Podophyllum hexandrum (Himalayan mayapple, Berberidaceae) was found to catalyze the cyclization of a dibenzylbutyrolactone lignan, yatein, to give deoxypodophyllotoxin and designated as deoxypodophyllotoxin synthase (DPS). Recently, we reported that the biosynthesis of deoxypodophyllotoxin and podophyllotoxin evolved in a lineage-specific manner in phylogenetically unrelated plant species such as P. hexandrum and Anthriscus sylvestris (cow parsley, Apiaceae). Therefore, a comprehensive understanding of the characteristics of DPSs that catalyze the cyclization of yatein to deoxypodophyllotoxin in various plant species is important. However, for plant species other than P. hexandrum, the isolation of the DPS enzyme gene and the type of the enzyme, e.g. whether it is 2-ODD or another type of enzyme such as cytochrome P-450, have not been reported. In this study, we report the identification and characterization of A. sylvestris DPS (AsDPS). Phylogenetic analysis showed that AsDPS belonged to the 2-ODD superfamily and shared moderate amino acid sequence identity (40.8%) with P. hexandrum deoxypodophyllotoxin synthase (PhDPS). Recombinant protein assay indicated that AsDPS and PhDPS differ in terms of the selectivity of substrate enantiomers. Protein modeling using AlphaFold2 and site-directed mutagenesis indicated that the Tyr305 residue of AsDPS probably contributes to substrate recognition. This study advances our understanding of the podophyllotoxin biosynthetic pathway in A. sylvestris and provides new insight into 2-ODD involved in plant secondary (specialized) metabolism.
(キーワード): Podophyllotoxin / Phylogeny / Lignans / Apiaceae
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/pcp/pcad103
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 37948767; ● Search Scopus @ Elsevier (PMID): 37948767; ● Search Scopus @ Elsevier (DOI): 10.1093/pcp/pcad103

(DOI: 10.1093/pcp/pcad103, PubMed: 37948767) Masaomi Yamamura, Kumatani Masato, Shiraishi Akira, Matsuura Yu, Kobayashi Keisuke, Suzuki Ayano, Kawamura Atsushi, Satake Honoo, Ragamustari Komara Safendrri, Suzuki Shiro, Suzuki Hideyuki, Shibata Daisuke, Kawai Shingo, Ono Eiichiro and Umezawa Toshiaki :
Two O-methyltransferases from Phylogenetically Unrelated Cow Parsley (Anthriscus sylvestris) and Hinoki-asunaro (Thujopsis dolabrata var. hondae) as a Signature of Lineage-specific Evolution in Lignan Biosynthesis,
Plant & Cell Physiology, Vol.64, No.1, 124-147, 2022.

(出版サイトへのリンク): ● Publication site (DOI): 10.1093/pcp/pcac164
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1093/pcp/pcac164

(DOI: 10.1093/pcp/pcac164) Andri Fadillah Martin, Yuki Tobimatsu, Pui Ying Lam, Naoyuki Matsumoto, Takuto Tanaka, Shiro Suzuki, Ryosuke Kusumi, Takuji Miyamoto, Yuri Takeda-Kimura, Masaomi Yamamura, Taichi Koshiba, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto and Toshiaki Umezawa :
Lignocellulose molecular assembly and deconstruction properties of lignin-altered rice mutants,
Plant Physiology, Vol.191, No.1, 70-86, 2022.

(要約): Bioengineering approaches to modify lignin content and structure in plant cell walls have shown promise for facilitating biochemical conversions of lignocellulosic biomass into valuable chemicals. Despite numerous research efforts, however, the effect of altered lignin chemistry on the supramolecular assembly of lignocellulose and consequently its deconstruction in lignin-modified transgenic and mutant plants is not fully understood. In this study, we aimed to close this gap by analyzing lignin-modified rice (Oryza sativa L.) mutants deficient in 5-HYDROXYCONIFERALDEHYDE O-METHYLTRANSFERASE (CAldOMT) and CINNAMYL ALCOHOL DEHYDROGENASE (CAD). A set of rice mutants harboring knockout mutations in either or both OsCAldOMT1 and OsCAD2 was generated in part by genome editing and subjected to comparative cell wall chemical and supramolecular structure analyses. In line with the proposed functions of CAldOMT and CAD in grass lignin biosynthesis, OsCAldOMT1-deficient mutant lines produced altered lignins depleted of syringyl and tricin units and incorporating noncanonical 5-hydroxyguaiacyl units, whereas OsCAD2-deficient mutant lines produced lignins incorporating noncanonical hydroxycinnamaldehyde-derived units. All tested OsCAldOMT1- and OsCAD2-deficient mutants, especially OsCAldOMT1-deficient lines, displayed enhanced cell wall saccharification efficiency. Solid-state nuclear magnetic resonance (NMR) and X-ray diffraction analyses of rice cell walls revealed that both OsCAldOMT1- and OsCAD2 deficiencies contributed to the disruptions of the cellulose crystalline network. Further, OsCAldOMT1 deficiency contributed to the increase of the cellulose molecular mobility more prominently than OsCAD2 deficiency, resulting in apparently more loosened lignocellulose molecular assembly. Such alterations in cell wall chemical and supramolecular structures may in part account for the variations of saccharification performance of the OsCAldOMT1- and OsCAD2-deficient rice mutants.
(キーワード): Lignin / Oryza / Plant Proteins / Mutation / Cell Wall
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/plphys/kiac432
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36124989; ● Summary page in Scopus @ Elsevier: 2-s2.0-85145424880

(DOI: 10.1093/plphys/kiac432, PubMed: 36124989, Elsevier: Scopus) Masaomi Yamamura, Takuji Miyamoto, Rie Takada, Dwi Widyajayantie, Vincentia Esti Windiastri, Satya Nugroho and Toshiaki Umezawa :
A microscale protocol for alkaline nitrobenzene oxidation of lignins using a readily available reactor,
Lignin, Vol.2, 19-24, 2021.

(出版サイトへのリンク): ● Publication site (DOI): 10.62840/lignin.2.0_19
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.62840/lignin.2.0_19

(DOI: 10.62840/lignin.2.0_19) Nge Thi Thi, Yamada Tatsuhiko, Tobimatsu Yuki, Masaomi Yamamura, Ishii Ryo, Tanaike Osamu and Ebina Takeo :
Fractionation and Characterization of Glycol Lignins by Stepwise-pH Precipitation of Japanese Cedar/Poly(ethylene glycol) Solvolysis Liquor,
ACS Sustainable Chemistry & Engineering, Vol.9, 756-764, 2021.

(出版サイトへのリンク): ● Publication site (DOI): 10.1021/acssuschemeng.0c06892
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85099706600

(DOI: 10.1021/acssuschemeng.0c06892, Elsevier: Scopus) Yonekura-Sakakibara Keiko, Masaomi Yamamura, Matsuda Fumio, Ono Eiichiro, Nakabayashi Ryo, Sugawara Satoko, Mori Tetsuya, Tobimatsu Yuki, Umezawa Toshiaki and Saito Kazuki :
Seed-coat protective neolignans are produced by the dirigent protein AtDP1 and the laccase AtLAC5 in Arabidopsis,
The Plant Cell, Vol.33, 129-152, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1093/plcell/koaa014
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85103433139

(DOI: 10.1093/plcell/koaa014, Elsevier: Scopus) Yoko Okahisa, Keisuke Kojiro, Hatsuki Ashiya, Takeru Tomita, Yuzo Furuta, Masaomi Yamamura and Toshiaki Umezawa :
Age-dependent and radial sectional differences in the dynamic viscoelastic properties of bamboo culms and their possible relationship with the lignin structures,
Journal of Wood Science, Vol.66, No.66, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s10086-020-01914-y
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1186/s10086-020-01914-y

(DOI: 10.1186/s10086-020-01914-y) Ramachandran Vasagi, Tobimatsu Yuki, Masaomi Yamamura, Sano Ryosuke, Umezawa Toshiaki, Demura Taku and Ohtani Misato :
Plant-specific Dof transcription factors VASCULAR-RELATED DOF1 and VASCULAR-RELATED DOF2 regulate vascular cell differentiation and lignin biosynthesis in Arabidopsis,
Plant Molecular Biology, Vol.104, 263-281, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11103-020-01040-9
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85088806821

(DOI: 10.1007/s11103-020-01040-9, Elsevier: Scopus) Nge Thi Thi, Tobimatsu Yuki, Masaomi Yamamura, Takahashi Shiho, Takata Eri, Umezawa Toshiaki and Yamada Tatsuhiko :
Effect of heat treatment on the chemical structure and thermal properties of softwood-derived glycol lignin.,
Molecules, Vol.25, 1167, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.3390/molecules25051167
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.3390/molecules25051167

(DOI: 10.3390/molecules25051167) Miyamoto Takuji, Takada Rie, Tobimatsu Yuki, Suzuki Shiro, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Sakamoto Masahiro and Umezawa Toshiaki :
Double knockout of OsWRKY36 and OsWRKY102 boosts lignification with altering culm morphology of rice.,
Plant Science, Vol.296, 110466, 2020.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.plantsci.2020.110466
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.plantsci.2020.110466

(DOI: 10.1016/j.plantsci.2020.110466) Martin Fadillah Andri, Tobimatsu Yuki, Kusumi Ryosuke, Matsumoto Naoyuki, Miyamoto Takuji, Lam Ying Pui, Masaomi Yamamura, Koshiba Taichi, Sakamoto Masahiro and Umezawa Toshiaki :
Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice.,
Scientific Reports, Vol.9, 17153, 2019.

(徳島大学機関リポジトリ): ● Metadata: 117772
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-019-53156-8
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-019-53156-8

(徳島大学機関リポジトリ: 117772, DOI: 10.1038/s41598-019-53156-8) Pui Ying Lam, Yuki Tobimatsu, Naoyuki Matsumoto, Shiro Suzuki, Wu Lan, Yuri Takeda, Masaomi Yamamura, Masahiro Sakamoto, John Ralph, Clive Lo and Toshiaki Umezawa :
OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls,
Scientific Reports, Vol.9, 1-13, 2019.

(徳島大学機関リポジトリ): ● Metadata: 117771
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-019-47957-0
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-019-47957-0

(徳島大学機関リポジトリ: 117771, DOI: 10.1038/s41598-019-47957-0) Shiro Suzuki, Hideyuki Suzuki, Koji Tanaka, Masaomi Yamamura, Daisuke Shibata and Toshiaki Umezawa :
De novo transcriptome analysis of needles of Thujopsis dolabrata var. hondae,
Plant Biotechnology, Vol.36, No.2, 113-118, 2019.

(要約): Podophyllotoxin is a starting material of the semisynthetic anticancer medicines etoposide, teniposide, and etopophos. The major plant source of podophyllotoxin is rhizomes of , which is a Himalayan endangered species; therefore, alternative sources of podophyllotoxin or bioproduction systems have been pursued to avoid exploiting this limited natural resource. In this paper, we report de novo transcriptome analysis of var. , which accumulates the podophyllotoxin derivatives (deoxypodophyllotoxin and β-peltatin A methyl ether) in its needles. We analyzed transcriptomes of the var. young needles to obtain the sequences that putatively encode -methyltransferases, cytochrome P450s, and a 2-oxoglutarate dependent dioxygenase because these protein families are responsible for podophyllotoxin-related compound formation in . The resulting transcriptomes contained considerable numbers of coding sequences classified into the three protein families. Our results are a genetic basis for identifying genes involved in the biosynthesis of podophyllotoxin and related compounds and also for future metabolic engineering of podophyllotoxin in heterologous hosts.
(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.19.0220a
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31768112; ● Summary page in Scopus @ Elsevier: 2-s2.0-85070918373

(DOI: 10.5511/plantbiotechnology.19.0220a, PubMed: 31768112, Elsevier: Scopus) Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Yuri Takeda, Suzuki Shiro, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto and Toshiaki Umezawa :
OsMYB108 loss-of-function enriches p-coumaroylated and tricin lignin units in rice cell walls.,
The Plant Journal : for Cell and Molecular Biology, Vol.98, No.6, 975-987, 2019.

(出版サイトへのリンク): ● Publication site (DOI): 10.1111/tpj.14290
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85063980966

(DOI: 10.1111/tpj.14290, Elsevier: Scopus) Pui Ying Lam, Andy C.W. Lui, Masaomi Yamamura, Lanxiang Wang, Yuri Takeda, Shiro Suzuki, Hongjia Liu, Fu-Yuan Zhu, Mo-Xian Chen, Jianhua Zhang, Umezawa Toshiaki, Yuki Tobimatsu and Clive Lo :
Recruitment of specific flavonoid B-ring hydroxylases for two independent biosynthesis pathways of flavone-derived metabolites in grasses,
The New Phytologist, Vol.223, No.1, 204-219, 2019.

(要約): In rice (Oryza sativa), OsF2H and OsFNSII direct flavanones to independent pathways that form soluble flavone C-glycosides and tricin-type metabolites (both soluble and lignin-bound), respectively. Production of soluble tricin metabolites requires CYP75B4 as a chrysoeriol 5'-hydroxylase. Meanwhile, the close homologue CYP75B3 is a canonical flavonoid 3'-hydroxylase (F3'H). However, their precise roles in the biosynthesis of soluble flavone C-glycosides and tricin-lignins in cell walls remain unknown. We examined CYP75B3 and CYP75B4 expression in vegetative tissues, analyzed extractable flavonoid profiles, cell wall structure and digestibility of their mutants, and investigated catalytic activities of CYP75B4 orthologues in grasses. CYP75B3 and CYP75B4 showed co-expression patterns with OsF2H and OsFNSII, respectively. CYP75B3 is the sole F3'H in flavone C-glycosides biosynthesis, whereas CYP75B4 alone provides sufficient 3',5'-hydroxylation for tricin-lignin deposition. CYP75B4 mutation results in production of apigenin-incorporated lignin and enhancement of cell wall digestibility. Moreover, tricin pathway-specific 3',5'-hydroxylation activities are conserved in sorghum CYP75B97 and switchgrass CYP75B11. CYP75B3 and CYP75B4 represent two different pathway-specific enzymes recruited together with OsF2H and OsFNSII, respectively. Interestingly, the OsF2H-CYP75B3 and OsFNSII-CYP75B4 pairs appear to be conserved in grasses. Finally, manipulation of tricin biosynthesis through CYP75B4 orthologues can be a promising strategy to improve digestibility of grass biomass for biofuel and biomaterial production.
(キーワード): Biosynthetic Pathways / Carbohydrate Metabolism / Cell Wall / Cytochrome P-450 Enzyme System / Flavones / Flavonoids / Gene Expression Regulation, Plant / Glycosides / Lignin / Magnetic Resonance Spectroscopy / Metabolome / Mixed Function Oxygenases / Mutation / Oryza / Panicum / Poaceae / Solubility / Sorghum
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/nph.15795
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30883799; ● Search Scopus @ Elsevier (PMID): 30883799; ● Search Scopus @ Elsevier (DOI): 10.1111/nph.15795

(DOI: 10.1111/nph.15795, PubMed: 30883799) Yuri Takeda, Yuki Tobimatsu, Masaomi Yamamura, Toshiyuki Takano, Masahiro Sakamoto and Toshiaki Umezawa :
Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition,
Journal of Wood Science, Vol.65, No.6, 1-11, 2019.

(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s10086-019-1784-6
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85061570581

(DOI: 10.1186/s10086-019-1784-6, Elsevier: Scopus) Takuji Miyamoto, Asako Mihashi, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Rie Takada, Yoshinori Kobayashi and Toshiaki Umezawa :
Comparative analysis of lignin chemical structures of sugarcane bagasse pretreated by alkaline, hydrothermal, and dilute sulfuric acid methods,
Industrial Crops and Products, Vol.121, 124-131, 2018.

(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.indcrop.2018.04.077
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85046808090

(DOI: 10.1016/j.indcrop.2018.04.077, Elsevier: Scopus) Takuji Miyamoto, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Miho Kojima, Keiji Takabe, Yoshifumi Terajima, Asako Mihashi, Yoshinori Kobayashi and Toshiaki Umezawa :
A comparative study of the biomass properties of Erianthus and sugarcane: lignocellulose structure, alkaline delignification rate, and enzymatic saccharification efficiency,
Bioscience, Biotechnology, and Biochemistry, Vol.82, No.7, 1143-1152, 2018.

(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09168451.2018.1447358
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85046716635

(DOI: 10.1080/09168451.2018.1447358, Elsevier: Scopus) Takeda Yuri, Tobimatsu Yuki, Karlen D. Steven, Koshiba Taichi, Suzuki Shiro, Masaomi Yamamura, Murakami Shinya, Mukai Mai, Takefumi Hattori, Keishi Osakabe, Ralph John, Sakamoto Masahiro and Umezawa Toshiaki :
Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification.,
The Plant Journal : for Cell and Molecular Biology, Vol.95, No.5, 796-811, 2018.

(出版サイトへのリンク): ● Publication site (DOI): 10.1111/tpj.13988
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85050919102

(DOI: 10.1111/tpj.13988, Elsevier: Scopus) Thi Thi Nge, Yuki Tobimatsu, Shiho Takahashi, Eri Takata, Masaomi Yamamura, Yasuyuki Miyagawa, Tsutomu Ikeda, Toshiaki Umezawa and Tatsuhiko Yamada :
Isolation and characterization of polyethylene glycol (PEG)-modified glycol lignin via PEG solvolysis of softwood biomass in a large-scale batch reactor,
ACS Sustainable Chemistry & Engineering, Vol.6, No.6, 7841-7848, 2018.

(出版サイトへのリンク): ● Publication site (DOI): 10.1021/acssuschemeng.8b00965
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85047075964

(DOI: 10.1021/acssuschemeng.8b00965, Elsevier: Scopus) Didi Tarmadi, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yasuyuki Miyagawa, Toshiaki Umezawa and Tsuyoshi Yoshimura :
NMR studies on lignocellulose deconstructions in the digestive system of the lower termite Coptotermes formosanus Shiraki.,
Scientific Reports, Vol.8, No.1, 1290, 2018.

(要約): Termites represent one of the most efficient lignocellulose decomposers on earth. The mechanism by which termites overcome the recalcitrant lignin barrier to gain access to embedded polysaccharides for assimilation and energy remains largely unknown. In the present study, softwood, hardwood, and grass lignocellulose diets were fed to Coptotermes formosanus workers, and structural differences between the original lignocellulose diets and the resulting feces were examined by solution-state multidimensional nuclear magnetic resonance (NMR) techniques as well as by complementary wet-chemical methods. Overall, our data support the view that lignin polymers are partially decomposed during their passage through the termite gut digestive system, although polysaccharide decomposition clearly dominates the overall lignocellulose deconstruction process and the majority of lignin polymers remain intact in the digestive residues. High-resolution NMR structural data suggested preferential removal of syringyl aromatic units in hardwood lignins, but non-acylated guaiacyl units as well as tricin end-units in grass lignins. In addition, our data suggest that termites and/or their gut symbionts may favor degradation of C-C-bonded β-5 and resinol-type β-β lignin inter-monomeric units over degradation of ether-bonded β-O-4 units, which is in contrast to what has been observed in typical lignin biodegradation undertaken by wood-decaying fungi.
(キーワード): Animals / Carbohydrate Sequence / Feces / Gastrointestinal Tract / Hydrolysis / Isoptera / Japan / Lignin / Magnetic Resonance Spectroscopy / Molecular Structure / Polysaccharides / Wood
(徳島大学機関リポジトリ): ● Metadata: 117770
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-018-19562-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29358744; ● Summary page in Scopus @ Elsevier: 2-s2.0-85040906500

(徳島大学機関リポジトリ: 117770, DOI: 10.1038/s41598-018-19562-0, PubMed: 29358744, Elsevier: Scopus) Didi Tarmadi, Tsuyoshi Yoshimura, Yuki Tobimatsu, Masaomi Yamamura and Toshiaki Umezawa :
Effects of lignins as diet components on the physiological activities of a lower termite, Coptotermes formosanus Shiraki,
Journal of Insect Physiology, Vol.103, 57-63, 2017.

(要約): We investigated the effects of lignins as diet components on the physiological activities of a lower termite, Coptotermes formosanus Shiraki. Artificial diets composed of polysaccharides with and without purified lignins (milled-wood lignins) from Japanese cedar (softwood), Japanese beech (hardwood), and rice (grass), were fed to C. formosanus workers. The survival and body mass of the workers as well as the presence of three symbiotic protists in the hindguts of the workers were then periodically examined. The survival rates of workers fed on diets containing lignins were, regardless of the lignocellulose diet sources, significantly higher than those of workers fed on only polysaccharides. In addition, it was clearly observed that all the tested lignins have positive effects on the maintenance of two major protists in the hindguts of C. formosanus workers, i.e., Pseudotrichonympha grassii and Holomastigotoides hartmanni. Overall, our data suggest that the presence of lignin is crucial to maintaining the physiological activities of C. formosanus workers during their lignocellulose decomposition. Our data also suggested that some components, possibly minerals and/or non-structural carbohydrates, in grass lignocellulose negatively affect the survival of C. formosanus workers as well as the present rate of the symbiotic protists in their hindguts.
(キーワード): Animals / Diet / Isoptera / Lignin / Parabasalidea / Symbiosis
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jinsphys.2017.10.006
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29038014; ● Summary page in Scopus @ Elsevier: 2-s2.0-85031815185

(DOI: 10.1016/j.jinsphys.2017.10.006, PubMed: 29038014, Elsevier: Scopus) Yuri Takeda, Taichi Koshiba, Yuki Tobimatsu, Shiro Suzuki, Shinya Murakami, Masaomi Yamamura, Md. Mahabubur Rahman, Toshiyuki Takano, Takefumi Hattori, Masahiro Sakamoto and Toshiaki Umezawa :
Regulation of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall lignin structure in rice,
Planta, Vol.246, No.2, 337-349, 2017.

(要約): Regulation of a gene encoding coniferaldehyde 5-hydroxylase leads to substantial alterations in lignin structure in rice cell walls, identifying a promising genetic engineering target for improving grass biomass utilization. The aromatic composition of lignin greatly affects utilization characteristics of lignocellulosic biomass and, therefore, has been one of the primary targets of cell wall engineering studies. Limited information is, however, available regarding lignin modifications in monocotyledonous grasses, despite the fact that grass lignocelluloses have a great potential for feedstocks of biofuel production and various biorefinery applications. Here, we report that manipulation of a gene encoding coniferaldehyde 5-hydroxylase (CAld5H, or ferulate 5-hydroxylase, F5H) leads to substantial alterations in syringyl (S)/guaiacyl (G) lignin aromatic composition in rice (Oryza sativa), a major model grass and commercially important crop. Among three CAld5H genes identified in rice, OsCAld5H1 (CYP84A5) appeared to be predominantly expressed in lignin-producing rice vegetative tissues. Down-regulation of OsCAld5H1 produced altered lignins largely enriched in G units, whereas up-regulation of OsCAld5H1 resulted in lignins enriched in S units, as revealed by a series of wet-chemical and NMR structural analyses. Our data collectively demonstrate that OsCAld5H1 expression is a major factor controlling S/G lignin composition in rice cell walls. Given that S/G lignin composition affects various biomass properties, we contemplate that manipulation of CAld5H gene expression represents a promising strategy to upgrade grass biomass for biorefinery applications.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00425-017-2692-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28421330; ● Summary page in Scopus @ Elsevier: 2-s2.0-85017652767

(DOI: 10.1007/s00425-017-2692-x, PubMed: 28421330, Elsevier: Scopus) Didi Tarmadi, Tsuyoshi Yoshimura, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yasuyuki Miyagawa and Toshiaki Umezawa :
The effects of various lignocelluloses and lignins on physiological responses of a lower termite, Coptotermes formosanus,
Journal of Wood Science, Vol.63, 464-472, 2017.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10086-017-1638-z
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85020509352

(DOI: 10.1007/s10086-017-1638-z, Elsevier: Scopus) Pui Ying Lam, Yuki Tobimatsu, Yuri Takeda, Masaomi Yamamura, Toshiaki Umezawa and Clive Lo :
Disrupting Flavone Synthase II alters lignin and improves biomass digestibility.,
Plant Physiology, Vol.174, No.2, 972-985, 2017.

(要約): Lignin, a ubiquitous phenylpropanoid polymer in vascular plant cell walls, is derived primarily from oxidative couplings of monolignols (-hydroxycinnamyl alcohols). It was discovered recently that a wide range of grasses, including cereals, utilize a member of the flavonoids, tricin (3',5'-dimethoxyflavone), as a natural comonomer with monolignols for cell wall lignification. Previously, we established that cytochrome P450 93G1 is a flavone synthase II (OsFNSII) indispensable for the biosynthesis of soluble tricin-derived metabolites in rice (). Here, our tricin-deficient mutant was analyzed further with an emphasis on its cell wall structure and properties. The mutant is similar in growth to wild-type control plants with normal vascular morphology. Chemical and nuclear magnetic resonance structural analyses demonstrated that the mutant lignin is completely devoid of tricin, indicating that FNSII activity is essential for the deposition of tricin-bound lignin in rice cell walls. The mutant also showed substantially reduced lignin content with decreased syringyl/guaiacyl lignin unit composition. Interestingly, the loss of tricin in the mutant lignin appears to be partially compensated by incorporating naringenin, which is a preferred substrate of OsFNSII. The mutant was further revealed to have enhanced enzymatic saccharification efficiency, suggesting that the cell wall recalcitrance of grass biomass may be reduced through the manipulation of the flavonoid monomer supply for lignification.
(キーワード): Biomass / Biosynthetic Pathways / Cell Wall / Fertility / Gene Expression Regulation, Plant / Gene Knockout Techniques / Genes, Plant / Lignin / Magnetic Resonance Spectroscopy / Mixed Function Oxygenases / Mutation / Oryza / Phenotype
(出版サイトへのリンク): ● Publication site (DOI): 10.1104/pp.16.01973
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28385728; ● Summary page in Scopus @ Elsevier: 2-s2.0-85020405754

(DOI: 10.1104/pp.16.01973, PubMed: 28385728, Elsevier: Scopus) Taichi Koshiba, Naoki Yamamoto, Yuki Tobimatsu, Masaomi Yamamura, Shiro Suzuki, Takefumi Hattori, Mai Mukai, Soichiro Noda, Daisuke Shibata, Masahiro Sakamoto and Toshiaki Umezawa :
MYB-mediated upregulation of lignin biosynthesis in Oryza Sativa towards biomass refinery,
Plant Biotechnology, Vol.34, No.1, 2017.

(要約): Lignin encrusts lignocellulose polysaccharides, and has long been considered an obstacle for the efficient use of polysaccharides during processes such as pulping and bioethanol fermentation. However, lignin is also a potential feedstock for aromatic products and is an important by-product of polysaccharide utilization. Therefore, producing biomass plant species exhibiting enhanced lignin production is an important breeding objective. Herein, we describe the development of transgenic rice plants with increased lignin content. Five (Arabidopsis) and one (rice) MYB transcription factor genes that were implicated to be involved in lignin biosynthesis were transformed into rice ( L. ssp. cv. Nipponbare). Among them, three Arabidopsis MYBs (, , and ) in transgenic rice T lines resulted in culms with lignin content about 1.5-fold higher than that of control plants. Furthermore, lignin structures in -overexpressing rice plants were investigated by wet-chemistry and two-dimensional nuclear magnetic resonance spectroscopy approaches. Our data suggested that heterologous expression of in rice increased lignin content mainly by enriching syringyl units as well as -coumarate and tricin moieties in the lignin polymers. We contemplate that this strategy is also applicable to lignin upregulation in large-sized grass biomass plants, such as , switchgrass, and .
(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.16.1201a
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31275003; ● Summary page in Scopus @ Elsevier: 2-s2.0-85016638801

(DOI: 10.5511/plantbiotechnology.16.1201a, PubMed: 31275003, Elsevier: Scopus) Tomoyuki Nakatsubo, Safendrri Komara Ragamustari, Takefumi Hattori, Eiichiro Ono, Masaomi Yamamura, Laigeng Li, Vincent L. Chiang and Toshiaki Umezawa :
A new O-methyltransferase for monolignol synthesis in Carthamus tinctorius.,
Plant Biotechnology, Vol.31, 545-553, 2014.

(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.14.0903a
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84923419892

(DOI: 10.5511/plantbiotechnology.14.0903a, Elsevier: Scopus) Safendrri komara Ragamustari, Masaomi Yamamura, Eiichiro Ono, Takefumi Hattori, Shiro Suzuki, Hideyuki Suzuki, Daisuke Shibata and Toshiaki Umezawa :
Substrate-enantiomer selectivity of matairesinol O-methyltransferases,
Plant Biotechnology, Vol.31, No.3, 257-267, 2014.

(要約): Previously we reported a cDNA encoding an O-methyltransferase (OMT) responsible for the O-methylation of matairesinol to afford arctigenin in Carthamus tinctorius. However, the regioselectivity and stereoselectivity of its reaction are not yet well understood. In this paper, we report the characterization and comparison of three matairesinol OMTs (MROMTs) encoded by cDNAs isolated from C. tinctorius (CtMROMT), Anthriscus sylvestris (AsMROMT), and Forsythia koreana (FkMROMT). Although they shared matairesinol as a substrate, each recombinant MROMT showed different catalytic behavior. AsMROMT and CtMROMT methylated matairesinol's hydroxyl group at the C4' position giving rise to arctigenin, while FkMROMT methylated the C4 position hydroxyl group giving rise to isoarctigenin. Analysis of the enantiomeric composition of products from racemic matairesinol showed all OMT reactions to be highly selective in terms of the substrate enantiomers and only use the (-)-enantiomer as the substrate.
(キーワード): lignan / O-methyltransferase (OMT) / Carthamus tinctorius / Anthriscus sylvestris / Forsythia koreana
(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.14.0722a
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204327186944; ● Summary page in Scopus @ Elsevier: 2-s2.0-84923387910

(DOI: 10.5511/plantbiotechnology.14.0722a, CiNii: 1390001204327186944, Elsevier: Scopus) Safendrri Komara Ragamustari, Tomoyuki Nakatsubo, Takefumi Hattori, Eiichiro Ono, Yu Kitamura, Shiro Suzuki, Masaomi Yamamura and Toshiaki Umezawa :
A novel O-methyltransferase involved in the first methylation step of yatein biosynthesis from matairesinol in Anthriscus sylvestris.,
Plant Biotechnology, Vol.30, 375-384, 2013.

(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.13.0527b
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84887871877

(DOI: 10.5511/plantbiotechnology.13.0527b, Elsevier: Scopus) Noda Soichiro, Takahashi Yoshinori, Tsurumaki Yuta, Masaomi Yamamura, Nishikubo Nobuyuki, Yamaguchi Masatoshi, Sakurai Nozomu, Suzuki Hideyuki, Demura Taku, Shibata Daisuke and Umezawa Toshiaki :
ATL54, a RING-H2 domain protein selected by a gene co-expression network analysis, is associated with secondary cell wall formation in Arabidopsis.,
Plant Biotechnology, Vol.30, 169-177, 2013. Taichi Koshiba, Norie Hirose, Mai Mukai, Masaomi Yamamura, Takefumi Hattori, Shiro Suzuki, Masahiro Sakamoto and Toshiaki Umezawa :
Characterization of 5-hydroxyconiferaldehyde O-methyltransferase in Oryza sativa,
Plant Biotechnology, Vol.30, 157-167, 2013.

(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.13.0219a
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84881059975

(DOI: 10.5511/plantbiotechnology.13.0219a, Elsevier: Scopus) Toshiaki Umezawa, Safendrri Komara Ragamustari, Tomoyuki Nakatsubo, Shohei Wada, Laigeng Li, Masaomi Yamamura, Norikazu Sakakibara, Takefumi Hattori, Shiro Suzuki and Vincent L. Chiang :
A lignan O-methyltransferase catalyzing the regioselective methylation of matairesinol in carthamus tinctorius,
Plant Biotechnology, Vol.30, No.2, 97-109, 2013.

(要約): Lignans are a group of plant phenolic compounds with various biological activities, including antitumor and antioxidant properties. O-Methylation is a critical step in biosynthesis of these compounds. However, little is known about the O-methyltransferase (OMT) enzymes that catalyze lignan O-methylation. We discovered a highly regioselective OMT activity in safflower (Carthamus tinctorius) seeds that catalyzed the methylation of matairesinol, a dibenzylbutyrolactone lignan, into 4'-O-methylmatairesinol (arctigenin) but not 4-O-methylmatairesinol (isoarctigenin). By examining such OMT activity in correlation with OMT transcript abundances during seed development, we cloned a few putative OMT cDNAs and produced their recombinant proteins in Escherichia coli. Among them, one protein exhibited O-methylation activity for matairesinol with the regioselectivity identical to that of the plant protein, and was named C. tinctorius matairesinol OMT (CtMROMT). CtMROMT did not show any detectable OMT activities towards phenylpropanoid monomers under the reaction conditions tested, while it methylated flavonoid apigenin efficiently into 4'-O-methylapigenin (acacetin). However, quantitative real-time polymerase chain reaction analysis demonstrated that expression of the CtMROMT gene was synchronized with the CtMROMT activity profile and arctigenin accumulation in the plant. These results demonstrated that CtMROMT is a novel plant OMT for lignan methylation.
(キーワード): Carthamus tinctorius / lignan / matairesinol / O-methyltransferase / regioselective methylation.
(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.12.1230a
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679304253568; ● Summary page in Scopus @ Elsevier: 2-s2.0-84881033781

(DOI: 10.5511/plantbiotechnology.12.1230a, CiNii: 1390282679304253568, Elsevier: Scopus) Masaomi Yamamura, Soichiro Noda, Takefumi Hattori, Amiu Shino, Jun Kikuchi, Keiji Takebe, Shuichiro Tagane, Mitsuru Gau, Naohiro Uwatoko, Masahiro Mii, Shiro Suzuki, Daisuke Shibata and Toshiaki Umezawa :
Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency.,
Plant Biotechnology, Vol.30, 25-35, 2013.

(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.12.1127a
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84878101694

(DOI: 10.5511/plantbiotechnology.12.1127a, Elsevier: Scopus) Masaomi Yamamura, Takefumi Hattori, Shiro Suzuki, Daisuke Shibata and Toshiaki Umezawa :
Microscale thioacidolysis method for the rapid analysis of β-O-4 substructures in lignin,
Plant Biotechnology, Vol.29, 419-423, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.12.0627a
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84868370324

(DOI: 10.5511/plantbiotechnology.12.0627a, Elsevier: Scopus) Masaomi Yamamura, Kinya Akashi, Akiho Yokota, Takefumi Hattori, Shiro Suzuki, Daisuke Shibata and Toshiaki Umezawa :
Characterization of Jatropha curcas lignins,
Plant Biotechnology, Vol.29, 179-183, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.12.0515b
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204327616768; ● Summary page in Scopus @ Elsevier: 2-s2.0-84864840302

(DOI: 10.5511/plantbiotechnology.12.0515b, CiNii: 1390001204327616768, Elsevier: Scopus) Masaomi Yamamura, Shohei Wada, Norikazu Sakakibara, Tomoyuki Nakatsubo, Shiro Suzuki, Takefumi Hattori, Migiwa Takeda, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata and Toshiaki Umezawa :
Occurrence of guaiacyl/p-hydroxyphenyl lignin in Arabidopsis thaliana T87 cells,
Plant Biotechnology, Vol.28, 1-8, 2011.

(キーワード): Arabidopsis thaliana / lignin / T87 cells
(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.10.0823c
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679305024896; ● Summary page in Scopus @ Elsevier: 2-s2.0-79955159516

(DOI: 10.5511/plantbiotechnology.10.0823c, CiNii: 1390282679305024896, Elsevier: Scopus) Masaomi Yamamura, Takefumi Hattori, Shiro Suzuki, Daisuke Shibata and Toshiaki Umezawa :
Microscale alkaline nitrobenzene oxidation method for high-throughput determination of lignin aromatic components.,
Plant Biotechnology, Vol.27, 305-310, 2010.

(要約): The nitrobenzene oxidation method is widely used for structural analysis of lignin. However, the conventional nitrobenzene oxidation method has several drawbacks including the requirement of a sizeable amount of sample material and the rather slow completion of the reaction process. In this paper, we describe a microscale nitrobenzene oxidation method using deuterium-labeled vanillin and syringaldehyde as internal standards. Using this method, we show that the microscale nitrobenzene oxidation method realized high-throughput determination of lignin components using a small amount of sample, i.e. 10 mg per reaction, and high reproducibility. Due to the small sample sizes, the oxidation reaction and extraction steps of a number of samples can be completed in parallel, thus enabling us to process more than 40 samples per day.
(キーワード): High-throughput / lignin / nitrobenzene oxidation
(出版サイトへのリンク): ● Publication site (DOI): 10.5511/plantbiotechnology.27.305
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204327677696; ● Summary page in Scopus @ Elsevier: 2-s2.0-78049516735

(DOI: 10.5511/plantbiotechnology.27.305, CiNii: 1390001204327677696, Elsevier: Scopus) Masaomi Yamamura, Shiro Suzuki, Takefumi Hattori and Toshiaki Umezawa :
Subunit composition of hinokiresinol synthase controls enantiomeric selectivity in hinokiresinol formation,
Organic & Biomolecular Chemistry, Vol.8, No.5, 1106-1110, 2010.

(要約): Asparagus officinalis hinokiresinol synthase (HRS) is composed of two subunits, HRSalpha and HRSbeta. Individually, each subunit forms (E)-hinokiresinol (EHR) from 4-coumaryl 4-coumarate, whereas a mixture of both subunits forms (Z)-hinokiresinol (ZHR) from the same substrate. In this study, we analyzed the enantiomeric compositions of ZHR and EHR formed after incubation of 4-coumaryl 4-coumarate with recombinant subunit proteins, recHRSalpha and/or recHRSbeta, and with naturally occurring A. officinalis ZHR. The enantiomeric composition of ZHR formed by the mixture of recHRSalpha and recHRSbeta was (+)-100% enantiomer excess (e.e.), identical to that of A. officinalis ZHR. In contrast, the enantiomeric compositions of EHR formed by recHRSalpha and recHRSbeta, individually, were (-)-20.6 and (-)-9.0% e.e., respectively. These results clearly demonstrate that the subunit composition of A. officinalis HRS controls not only cis/trans isomerism but also enantioselectivity in hinokiresinol formation.
(キーワード): Asparagus Plant / Isomerism / Ligases / Lignans / Phenols / Protein Subunits
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/B918656E
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20165801; ● Search Scopus @ Elsevier (PMID): 20165801; ● Search Scopus @ Elsevier (DOI): 10.1039/B918656E

(DOI: 10.1039/B918656E, PubMed: 20165801) Shiro Suzuki, Masaomi Yamamura, Takefumi Hattori, Tomoyuki Nakatsubo and Toshiaki Umezawa :
The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation.,
Proceedings of the National Academy of Sciences of the United States of America, Vol.104, No.52, 21008-21013, 2007.

(要約): The selective formation of E- or Z-isomers is an important process in natural product metabolism. We show that the subunit composition of an enzyme can alter the geometrical composition of the enzymatic products. Hinokiresinol synthase, purified from Asparagus officinalis cell cultures, is responsible for the conversion of (7E,7'E)-4-coumaryl 4-coumarate to (Z)-hinokiresinol, the first step in norlignan formation. The protein is most likely a heterodimer composed of two distinct subunits, which share identity with members of the phloem protein 2 gene superfamily. Interestingly, each recombinant subunit of hinokiresinol synthase expressed in Escherichia coli solely converted (7E,7'E)-4-coumaryl 4-coumarate to the unnatural (E)-hinokiresinol, the E-isomer of (Z)-hinokiresinol. By contrast, a mixture of recombinant subunits catalyzed the formation of (Z)-hinokiresinol from the same substrate.
(キーワード): Acyl Coenzyme A / Asparagus Plant / Cells, Cultured / Cloning, Molecular / DNA, Complementary / Dimerization / 大腸菌 (Escherichia coli) / Gas Chromatography-Mass Spectrometry / Lignans / 質量分析法 (mass spectrometry) / Models, Chemical / Molecular Sequence Data / Phenols / Phylogeny / Plant Lectins
(出版サイトへのリンク): ● Publication site (DOI): 10.1073/pnas.0710357105
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18093914; ● Summary page in Scopus @ Elsevier: 2-s2.0-38049100715

(DOI: 10.1073/pnas.0710357105, PubMed: 18093914, Elsevier: Scopus) Shiro Suzuki, Masaomi Yamamura, Mikio Shimada and Toshiaki Umezawa :
A heartwood norlignan, (E)-hinokiresinol, is formed from 4-coumaryl 4-coumarate by a Cryptomeria japonica enzyme preparation,
Chemical Communications, Vol.24, 2838-2839, 2004.

(要約): An enzyme preparation from the cultured cells of Cryptomeria japonica catalyses the formation of a heartwood norlignan, (E)-hinokiresinol, from two distinct phenylpropanoid monomers: 4-coumaroyl CoA and 4-coumaryl alcohol, and from a dimer: 4-coumaryl 4-coumarate.
(キーワード): Cells, Cultured / Coumaric Acids / Cryptomeria / Lignans / Mass Spectrometry / Molecular Structure / Phenols
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/b409686j
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15599432; ● Search Scopus @ Elsevier (PMID): 15599432; ● Search Scopus @ Elsevier (DOI): 10.1039/b409686j

(DOI: 10.1039/b409686j, PubMed: 15599432)

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総説・解説

Toshiaki Umezawa, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yuri Takeda, Taichi Koshiba, Rie Takada, Pui Ying Lam, Shiro Suzuki and Masahiro Sakamoto :
Lignin metabolic engineering in Grasses for primary lignin valorization,
Lignin, Vol.1, 30-41, Sep. 2020.

(キーワード): Grass / Lignin / Metabolic engineering / Structural simplification / Upregulation
(出版サイトへのリンク): ● Publication site (DOI): 10.62840/lignin.1.0_30
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.62840/lignin.1.0_30

(DOI: 10.62840/lignin.1.0_30) Toshiaki Umezawa, Masaomi Yamamura, Eiichiro Ono, Akira Shiraishi and Safendrri Komara Ragamustari :
O-Methyltransferases involved in lignan biosynthesis,
木材学会誌, Vol.65, No.1, 1-12, Jan. 2019.

(出版サイトへのリンク): ● Publication site (DOI): 10.2488/jwrs.65.1
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2488/jwrs.65.1

(DOI: 10.2488/jwrs.65.1) Umezawa Toshiaki, Ragamustari Komara Safendrri, Ono Eiichiro and Masaomi Yamamura :
O-Methyltransferases involved in lignan biosynthesis.,
Recent Advances in Phytochemistry, Vol.45, 99-114, 2015.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/978-3-319-20397-3_4
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1007/978-3-319-20397-3_4

(DOI: 10.1007/978-3-319-20397-3_4) Toshiaki Umezawa, Masaomi Yamamura, Tomoyuki Nakatsubo, Shiro Suzuki and Takefumi Hattori :
Stereoselectivity of the Biosynthesis of Norlignans and Related Compounds,
Recent Advances in Phytochemistry, Vol.41, 179-197, Nov. 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/978-1-4419-7299-6_12
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1007/978-1-4419-7299-6_12

(DOI: 10.1007/978-1-4419-7299-6_12) 鈴木史朗, 山村正臣, 梅澤俊明 :
ヒノキレジノール合成酵素,
化学と生物, Vol.46, No.9, 592-594, 2008年9月.

講演・発表

Takefumi Hattori, Katayama Megumi, Tsuzuki Hiromitsu, Okamoto Yumi, Ida Kyosuke, Yoshizumi Mariko, Abe Masanori, Ryushi Kawakami, Junji Hayashi and Masaomi Yamamura :
Phenylpropanoid metabolism in basidiomycete Tricholoma matsutake -cDNA cloning of Sadenosyl-L-methionine-dependent cinnamic acid carboxyl methyltransferase-,
2nd International Lignin Symposium (Kyoto), Sep. 2024. Kobayashi Keisuke, Suyama Rinano, Mikami Bunzo, Masaomi Yamamura and Umezawa Toshiaki :
Selective loss of function of lignan O-methylation activity in lignan/flavonoid O-methyltransferase,
Annual conference of the Phytochemical Society of North America 2024, Monterrey, Mexico, Jul. 2024. Hirota Mitsuki, Tutihashi Satoshi Fernando, Masaomi Yamamura, Kobayashi Keisuke, Tobimatsu Yuki, Hamberger Björn and Umezawa Toshiaki :
Pinoresinol/lariciresinol reductase and secoisolariciresinol dehydrogenase involved in specific production of the (+)-enantiomer of matairesinol in Daphne genkwa,
Annual Conference of the Phytochemical Society of North America 2023, Jul. 2023. Masaomi Yamamura, Kumatani Masato, Shiraishi Akira, Matsuura Yu, Kobayashi Keisuke, Suzuki Ayano, Kawamura Atsushi, Satake Honoo, Ragamustari Komara Safendrri, Suzuki Shiro, Suzuki Hideyuki, Shibata Daisuke, Kawai Shingo, Ono Eiichiro and Umezawa Toshiaki :
Two O-methyltransferases from phylogenetically unrelated Anthriscus sylvestris and Thujopsis dolabrata var. hondae as a signature of lineage-specific evolution in aryltetralin lignan biosynthesis,
61st Annual Meeting of the Phytochemical Society of North America (PSNA), Blacksburg, VA, US, July 24-28, 2022, Jul. 2022. Yonekura-Sakakibara Keiko, Masaomi Yamamura, Matsuda Fumio, Ono Eiichiro, Nakabayashi Ryo, Sugawara Satoko, Mori Tetsuya, Tobimatsu Yuki, Umezawa Toshiaki and Saito Kazuki :
Seed-coat protective neolignan biosynthesis in Arabidopsis,
Pacifichem 2021 (on-line), Dec16-21, 2021, Dec. 2021. Miyamoto Takuji, Takada Rie, Tobimatsu Yuki, Suzuki Shiro, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Sakamoto Masahiro and Umezawa Toshiaki :
Knockout of OsWRKY36 and OsWRKY102 boosts lignification with altering culm morphology of rice,
60th Annual Meeting of the Phytochemical Society of North America, July 25-30, 2021 (Kelowna, Canada,on-line), Jul. 2021. 小林慶亮, 陶山莉菜乃, 三上文三, 山村正臣, 梅澤俊明 :
リグナン/フラボノイドOMTにおけるリグナンメチル化活性の選択的機能破壊,
第41回日本植物バイオテクノロジー学会(仙台)大会, 2024年9月. 服部武文, 片山恵, 川上竜巳, 林順司, 山村正臣 :
マツタケ由来S-アデノシル-L-メチオニン依存ケイ皮酸カルボキシルメチルトランスフェラーゼ組換え酵素の特性解明,
第74回日本木材学会大会(京都大会), 2024年3月. 小林慶亮, 山村正臣, 三上文三, 白石慧, 佐竹炎, 小埜栄一郎, 梅澤俊明 :
シャクにおけるデオキシポドフィロトキシン合成酵素の機能解析,
第40回日本植物バイオテクノロジー学会(千葉)大会, 2023年9月. Tutihashi Satoshi Fernando, Hirota Mitsuki, Kobayashi Keisuke, Masaomi Yamamura, Tobimatsu Yuki, Hamberger Bjorn and Umezawa Toshiaki :
Mechanisms for the substrate enantiomer selectivity of pinoresinol/lariciresinol reductases from Daphne Genkwa.,
第40回日本植物バイオテクノロジー学会(千葉)大会, Sep. 2023. 山村正臣 :
シャクを用いたリグナン生合成研究,
芦生 Open Science Meeting 2023, 2023年3月. 服部武文, 片山恵, 井田京介, 岡本有未, 山村正臣, 吉住真理子, 阿部正範 :
マツタケ由来ケイ皮酸メチル化酵素の組換え酵素調製方法の改良 ―超音波破砕で得た不溶性画分からの酵素抽出―,
第73回日本木材学会(福岡大会), 2023年3月. Hirota Mitsuki, Tutihashi Satoshi Fernando, Masaomi Yamamura, Kobayashi Keisuke, Tobimatsu Yuki, Hamberger Bjorn, Buell Robin Carol and Umezawa Toshiaki :
Characterization of Pinoresinol/Lariciresinol Reductase and Secoisolariciresinol Dehydrogenase from Daphne genkwa,
第73回日本木材学会(福岡大会), Mar. 2023. 小林慶亮, 三上文三, 陶山莉菜乃, 山村正臣, 梅澤俊明 :
ベニバナ由来の matairesinol O-methyltransferase の機能解析,
第73回日本木材学会(福岡大会), 2023年3月. 吉住真理子, 阿部正範, 岩井健人, 在間航也, 片山恵, 山村正臣, 服部武文 :
スギオガコを用いたキクラゲ菌床栽培,
日本きのこ学会第25回大会, 2022年9月. 小林慶亮, 山村正臣, 小埜栄一郎, 白石慧, 佐竹炎, 梅澤俊明 :
シャクにおけるyateinの環化に関与する2-oxoglutarate-dependent dioxygenase (As2-ODD)の機能解析,
第39回日本植物バイオテクノロジー学会(堺)大会, 2022年9月. 榊原圭子, 山村正臣, 松田史生, 小埜栄一郎, 中林亮, 菅原聡子, 森哲哉, 飛松裕基, 梅澤俊明, 斎藤和季 :
シロイヌナズナの種子特異的ネオリグナンの生合成に関わるディリジェントプロテインとラッカーゼ,
第66回リグニン討論会(リグニン学会第3回年次大会) オンライン開催 2021年11月4日(木)∼5日(金), 2021年11月. 小林慶亮, 山村正臣, 熊谷真聡, 小埜栄一郎, 白石慧, 佐竹炎, 梅澤俊明 :
Yateinの環化を触媒するシャク由来の新規の2-oxoglutarate-dependent dioxygenaseの同定,
第38回日本植物バイオテクノロジー学会(つくば)大会オンライン開催 2021年9月9日(木)∼11日(土), 2021年9月. 小林慶亮, 山村正臣, 熊谷真聡, 小埜栄一郎, 白石慧, 佐竹炎, 梅澤俊明 :
シャクにおける yateinの環化に関与する新規の2-oxoglutarate-dependent dioxygenaseの同定,
第71回日本木材学会大会(東京・オンライン) 2021年3月19日-21日, 2021年3月.

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補助金・競争的資金: 担子菌特異的カルボキシルメチルトランスフェラーゼの反応機作の解明 (研究課題/領域番号: 23K05337 )
針葉樹心材ノルリグナン生合成遺伝子の同定 (研究課題/領域番号: 22K20593 )
統合ゲノム解析に基づく抗腫瘍性リグナン生合成機構の解明 (研究課題/領域番号: 23K23671 )
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専門分野・研究分野: 森林植物代謝学
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受賞: 2009年5月, 日本農芸化学会関西支部第459回講演会若手優秀発表賞 (社団法人日本農芸化学会)
活動: とくしま木づかい県民会議 (幹事 [2022年4月〜2025年3月])
徳島県立那賀高等学校学校運営協議会 (運営協議会委員 [2022年5月〜2025年3月])
徳島大学大学院社会産業理工学研究部理工学域及び生物資源産業学域研究倫理委員会 (2022年4月〜2024年3月)
高等教育研究センター・学修支援部門創新教育推進班兼教員・創新教育推進班イノベーション教育支援教員 (2023年4月〜2024年3月)

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Jグローバル最終確認日: 2024/12/21 01:07
氏名（漢字）: 山村正臣
氏名（フリガナ）: ヤマムラマサオミ
氏名（英字）: Yamamura Masaomi
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researchmap最終確認日: 2024/12/22 04:00
氏名（漢字）: 山村正臣
氏名（フリガナ）: ヤマムラマサオミ
氏名（英字）: Yamamura Masaomi
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