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松尾敬志

徳島大学

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2024年4月26日更新

職名: 名誉教授 (2019.4)・教授
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学歴: 1980/3: 大阪大学歯学部歯学科卒業
1984/3: 大阪大学大学院歯学研究科博士課程修了
学位: 歯学博士 (大阪大学) (1984年3月)
職歴・経歴: 1984/4: 大阪大学歯学部助手
1990/4: 徳島大学歯学部附属病院講師
1993/11: 徳島大学歯学部助教授
1996/6: 徳島大学歯学部教授
2004/4: 徳島大学大学院ヘルスバイオサイエンス研究部発達予防医歯学部門健康長寿歯科学講座歯科保存学分野教授

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)

研究者総覧で最新データを確認する

2024年4月26日更新

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)
担当経験のある授業科目: 研究者総覧に該当データはありませんでした。
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2024年4月26日更新

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)

研究テーマ: 齲蝕に関する研究:齲蝕細菌封じ込め法を応用した新しい治療法の開発 (齲蝕)
歯髄疾患に関する研究:歯髄炎の不可逆性化に関する研究 (歯髄炎)
根尖性歯周炎に関する研究:感染根管における細菌侵襲の実態 (感染根管)

著書

中西正, 松尾敬志 :
2. 感染根管における細菌感染の実態,
株式会社ヒョーロン・パブリッシャーズ, 2019年6月. 松尾敬志, 中西正 :
第17章高齢者・有病者の歯内治療,
医歯薬出版株式会社, 2018年9月. 松尾敬志, 中西正 :
第1章歯と歯根周囲の組織学,
永末書店, 2018年1月. 松尾敬志 :
エンドドンティクス,
永末書店, 京都, 2015年1月. 松尾敬志 :
保存修復学21,
永末書店, 京都, 2011年4月. 松尾敬志 :
保存修復学21,
永末書店, 2011年4月. Tadashi Nakanishi, Kanako Takahashi, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Proceedings of the International Conference on Dentin/Pulp Complex 2001, --- An Immunohistologic Study on the Localization of Selected Bacteria and Chemokines in Human Deep-Carious Teeth ---,
Quintessence Publishing CO.,Ltd., Tokyo, Jun. 2002. 松尾敬志, 吉山昌宏, 尾崎和美, 中江英明 :
第2章有髄歯への対応根面齲蝕への対応:シールドストレーションの応用,
ヒョーロン·パブリッシャーズ, 東京, 2000年8月.

(キーワード): 象牙質齲蝕 / 先駆細菌 / シールドトリートメント / STOC / シールドストレーション

論文

Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Nobiletin Inhibits Inflammatory Reaction in Interleukin-1β-Stimulated Human Periodontal Ligament Cells.,
Pharmaceutics, Vol.13, No.5, 667, 2021.

(要約): The immune response in periodontal lesions is involved in the progression of periodontal disease. Therefore, it is important to find a bioactive substance that has anti-inflammatory effects in periodontal lesions. This study aimed to examine if nobiletin, which is found in the peel of citrus fruits, could inhibit inflammatory responses in interleukin (IL)-1β-stimulated human periodontal ligament cells (HPDLCs). The release of cytokines (IL-6, IL-8, CXCL10, CCL20, and CCL2) and matrix metalloproteinases (MMP-1 and MMP-3) was assessed by ELISA. The expression of cell adhesion molecules (ICAM-1and VCAM-1) and the activation of signal transduction pathways (nuclear factor (NF)-κB, mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt)) in HPDLCs were detected by Western blot analysis. Our experiments revealed that nobiletin decreased the expression of inflammatory cytokines, cell adhesion molecules, and MMPs in IL-1β-stimulated HPDLCs. Moreover, we revealed that nobiletin treatment could suppress the activation of the NF-κB, MAPKs, and Akt pathways. These findings indicate that nobiletin could inhibit inflammatory reactions in IL-1β-stimulated HPDLCs by inhibiting multiple signal transduction pathways, including NF-κB, MAPKs, and Akt.
(徳島大学機関リポジトリ): ● Metadata: 116619
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/pharmaceutics13050667
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34066937; ● Search Scopus @ Elsevier (PMID): 34066937; ● Search Scopus @ Elsevier (DOI): 10.3390/pharmaceutics13050667

(徳島大学機関リポジトリ: 116619, DOI: 10.3390/pharmaceutics13050667, PubMed: 34066937) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
The Polymethoxy Flavonoid Sudachitin Inhibits Interleukin-1 β-Induced Inflammatory Mediator Production in Human Periodontal Ligament Cells,
BioMed Research International, Vol.2021, No.Article ID 8826586, 2021.

(徳島大学機関リポジトリ): ● Metadata: 116275
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2021/8826586
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33575345; ● Search Scopus @ Elsevier (PMID): 33575345; ● Search Scopus @ Elsevier (DOI): 10.1155/2021/8826586

(徳島大学機関リポジトリ: 116275, DOI: 10.1155/2021/8826586, PubMed: 33575345) Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Carnosic acid inhibits inflammatory cytokines production in human periodontal ligament cells.,
Immunopharmacology and Immunotoxicology, Vol.42, No.4, 373-378, 2020.

(要約): The results of this study suggest that CA has anti-inflammatory effects in human periodontal ligament cells by inhibiting JNK, NF-κB and STAT3 pathways.
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/08923973.2020.1782427
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32538208; ● Summary page in Scopus @ Elsevier: 2-s2.0-85087515653

(DOI: 10.1080/08923973.2020.1782427, PubMed: 32538208, Elsevier: Scopus) Hitomi Kuramoto, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa, Tadashi Nakanishi, Daisuke Takegawa, Ayako Washio, Chiaki Kitamura and Takashi Matsuo :
Caffeic acid phenethyl ester (CAPE) induces VEGF expression and production in rat odontoblastic cells,
BioMed Research International, Vol.Article ID 5390720, 2019.

(要約): Caffeic acid phenethyl ester (CAPE), the main component of propolis, has various biological activities including anti-inflammatory effect and wound healing promotion. Odontoblasts located in the outermost layer of dental pulp play crucial roles such as production of growth factors and formation of hard tissue termed reparative dentin in host defense against dental caries. In this study, we investigated the effects of CAPE on the upregulation of vascular endothelial growth factor (VEGF) and calcification activities of odontoblasts, leading to development of novel therapy for dental pulp inflammation caused by dental caries. CAPE significantly induced mRNA expression and production of VEGF in rat clonal odontoblast-like KN-3 cells cultured in normal medium or osteogenic induction medium. CAPE treatment enhanced nuclear factor-kappa B (NF-B) transcription factor activation, and furthermore, the specific inhibitor of NF-B significantly reduced VEGF production. The expression of VEGF receptor- (VEGFR-) 2, not VEGFR-1, was up regulated in KN-3 cells treated with CAPE. In addition, VEGF significantly increased mineralization activity in KN-3 cells. These findings suggest that CAPE might be useful as a novel biological material for the dental pulp conservative therapy.
(徳島大学機関リポジトリ): ● Metadata: 114566
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2019/5390720
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31930126; ● Summary page in Scopus @ Elsevier: 2-s2.0-85077441042

(徳島大学機関リポジトリ: 114566, DOI: 10.1155/2019/5390720, PubMed: 31930126, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Sudachitin Inhibits Matrix Metalloproteinase-1 and -3 Production in Tumor Necrosis Factor-α-Stimulated Human Periodontal Ligament Cells.,
Inflammation, Vol.42, No.4, 1456-1462, 2019.

(要約): Sudachitin, a polymethoxylated flavonoid found in the skin of Citrus sudachi, is a biologically active substance. The aim of this study was to examine whether sudachitin could be used to inhibit the expression of matrix metalloproteinase (MMP)-1 and MMP-3, which are involved in the destruction of periodontal tissues in periodontal lesions, in tumor necrosis factor (TNF)-α-stimulated human periodontal ligament cells (HPDLC). Sudachitin suppressed TNF-α-induced MMP-1 and MMP-3 production in HPDLC. On the other hand, it enhanced tissue inhibitor of metalloproteinase (TIMP)-1 expression. The level of Akt phosphorylation in the TNF-α-stimulated HPDLC was decreased by sudachitin treatment. Moreover, an Akt inhibitor reduced MMP-1 and MMP-3 production and increased TIMP-1 production. These findings indicate that sudachitin reduces MMP-1 and MMP-3 production in TNF-α-stimulated HPDLC by inhibiting the Akt pathway.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-019-01007-z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30997585; ● Search Scopus @ Elsevier (PMID): 30997585; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-019-01007-z

(DOI: 10.1007/s10753-019-01007-z, PubMed: 30997585) Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Carnosic Acid Inhibits CXCR3 Ligands Production in IL-27-Stimulated Human Oral Epithelial Cells.,
Inflammation, Vol.42, No.4, 1311-1316, 2019.

(要約): Carnosic acid, which is a bioactive compound isolated from rosemary, has various pharmacological effects. However, the anti-inflammatory effect of carnosic acid on periodontitis is still unknown. The aim of this study was to investigate the effect of carnosic acid on CXC chemokine receptor 3 (CXCR3) ligands, which are involved in Th1 cells migration and accumulation, production in interleukin (IL)-27-stimulated human oral epithelial cells (TR146 cells). Carnosic acid decreased CXC chemokine ligand (CXCL)9, CXCL10, and CXCL11 production in IL-27-stimulated TR146 cells in a dose-dependent fashion. Moreover, we disclosed that carnosic acid could suppress signal transducer and activator of transcription (STAT)1, STAT3, and protein kinase B (Akt) phosphorylation in IL-27-stimulated TR146 cells. Furthermore, STAT1, STAT3, and Akt inhibitors could suppress CXCR3 ligands production in IL-27-treated TR146 cells. In summary, carnosic acid could reduce CXCR3 ligands production in human oral epithelial cell by inhibiting STAT1, STAT3, and Akt activation.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-019-00991-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30820808; ● Search Scopus @ Elsevier (PMID): 30820808; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-019-00991-6

(DOI: 10.1007/s10753-019-00991-6, PubMed: 30820808) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Honokiol and Magnolol Inhibit CXCL10 and CXCL11 Production in IL-27-Stimulated Human Oral Epithelial Cells.,
Inflammation, Vol.41, No.6, 2110-2115, 2018.

(要約): Honokiol and magnolol, which are lignans isolated from Magnolia quinquepeta, have some pharmacological effects. However, the anti-inflammatory effects of honokiol and magnolol on periodontal disease are still uncertain. The aim of this study was to examine the effect of honokiol and magnolol on CXC chemokine receptor 3 (CXCR3) ligands, which are related with Th1 cell migration, production in interleukin (IL)-27-stimulated human oral epithelial cells (TR146 cells). Honokiol and magnolol inhibited CXC chemokine ligand (CXCL)10 and CXCL11 production in IL-27-stimulated TR146 cells in a dose-dependent manner. Moreover, we revealed that honokiol and magnolol could suppress signal transducer and activator of transcription (STAT)3 and protein kinase B (Akt) phosphorylation in IL-27-stimulated TR146 cells though STAT1 phosphorylation was not suppressed by honokiol and magnolol treatment. Furthermore, STAT3 and Akt inhibitors could suppress CXCR3 ligand production in TR146 cells. In summary, honokiol and magnolol could reduce CXCR3 ligand production in oral epithelial cell by inhibiting STAT3 and Akt activation.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-018-0854-z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30039429; ● Search Scopus @ Elsevier (PMID): 30039429; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-018-0854-z

(DOI: 10.1007/s10753-018-0854-z, PubMed: 30039429) Yoshihito Naitou, Hiromichi Yumoto, K Hs Kumar, Takashi Matsuo, Katsuhiko Hirota, Yoichiro Miyake, Kan Nagao, Yoritoki Tomotake, R Jimbo and Tetsuo Ichikawa :
Antifungal and Mechanical Properties of Tissue Conditioner Containing Plant-Derived Component, --- An In Vitro Study ---,
Journal of Prosthodontics, Vol.27, No.7, 665-669, 2018.

(要約): To evaluate the antifungal activity and mechanical properties of a novel antifungal tissue conditioner containing Juncus powder. Juncus powder was mixed with GC tissue conditioner at concentrations of 2.5%, 5.0%, and 10.0% by mass. The cylindrical specimens of Juncus-mixed tissue conditioner (dimensions: 10 mm in diameter and 2 and 6 mm in height for antimicrobial and mechanical tests, respectively) were prepared. The specimens placed on the bottom of the 24-well tissue culture plate were cultured with Candida albicans CAD1 for 2 and 4 days. The proliferation of the C. albicans in the wells was determined by measuring the optical density of fungal culture, and the surface of the specimens were also observed by scanning electron microscopy (SEM). To assess the mechanical properties of the specimens, the fluidity and hardness of Juncus-mixed tissue conditioner were measured using the methods certified according to ISO 10139-1. Juncus-mixed tissue conditioner significantly exhibited growth inhibitory effect in a Juncus concentration-dependent manner after both 2- and 4- day cultures. SEM observation showed that the amount of C. albicans on Juncus-mixed specimens drastically decreased, and biofilm formation was markedly inhibited. Moreover, both mechanical properties were found to be within the ranges regulated and specified by ISO. These findings demonstrated that the tissue conditioner including Juncus powder has a significant growth inhibitory effect against C. albicans, and it is suggested that the application of Juncus-mixed tissue conditioner may prevent denture stomatitis and oral candidiasis in denture wearers.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jopr.12546
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28901663; ● Search Scopus @ Elsevier (PMID): 28901663; ● Search Scopus @ Elsevier (DOI): 10.1111/jopr.12546

(DOI: 10.1111/jopr.12546, PubMed: 28901663) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Transforming growth factor-β1 increases C-C chemokine ligand 11 production in interleukin 4-stimulated human periodontal ligament cells.,
Cell Biology International, Vol.42, No.10, 1395-1400, 2018.

(要約): Transforming growth factor (TGF)-β1 is a multifunctional cytokine, which can control certain functions of various kinds of cells. However, it is unclear whether TGF-β1 affects T-cell migration in periodontal lesions. The aim of this study was to examine the effects of TGF-β1 on the production of C-C chemokine ligand (CCL)11, which is a T-helper 2-type chemokine, in human periodontal ligament cells (HPDLC). Interleukin (IL)-4 induced CCL11 production, but TGF-β1 did not, in HPDLC. However, TGF-β1 enhanced CCL11 production in IL-4-stimulated HPDLC. Western blot analysis showed that the signal transducer and activator of transcription 6 (STAT6) pathway was highly activated in HPDLC that had been stimulated with both IL-4 and TGF-β1. Mitogen-activated protein kinase activation did not differ between the HPDLC treated with a combination of IL-4 and TGF-β1 and those treated with IL-4 or TGF-β1 alone. Moreover, a STAT6 inhibitor significantly inhibited CCL11 production in HPDLC that had been stimulated with IL-4 and TGF-β1. The current study clearly demonstrated that TGF-β1 enhanced IL-4-induced CCL11 production in HPDLC. The STAT6 pathway is important for CCL11 production in IL-4- and TGF-β1-treated HPDLC.
(キーワード): Cells, Cultured / Chemokine CCL11 / Chemokines, CC / 細胞質分裂 (cytokinesis) / Humans / Interleukin-4 / Periodontal Ligament / リン酸化 (phosphorylation) / STAT6 Transcription Factor / シグナル伝達 (signal transduction) / Transforming Growth Factor beta1
(徳島大学機関リポジトリ): ● Metadata: 112813
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/cbin.11030
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29993161; ● Search Scopus @ Elsevier (PMID): 29993161; ● Search Scopus @ Elsevier (DOI): 10.1002/cbin.11030

(徳島大学機関リポジトリ: 112813, DOI: 10.1002/cbin.11030, PubMed: 29993161) 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
テアフラビンが口腔上皮細胞のケモカイン産生に与える影響の解析,
日本歯科保存学雑誌, Vol.61, No.1, 10-16, 2018年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.61.10
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680499546240; ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.61.10

(DOI: 10.11471/shikahozon.61.10, CiNii: 1390282680499546240) 平尾功治, 湯本浩通, 細川由樹, 蔵本瞳, 鷲尾絢子, 中西正, 武川大輔, 北村知昭, 松尾敬志 :
ラット象牙芽細胞様細胞 (KN-3) におけるカテキンの抗炎症作用,
日本歯科保存学雑誌, Vol.60, No.5, 235-244, 2017年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.60.235
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680498132224; ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.60.235

(DOI: 10.11471/shikahozon.60.235, CiNii: 1390282680498132224) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
IL-27 Modulates Chemokine Production in TNF- -Stimulated Human Oral Epithelial Cells.,
Cellular Physiology and Biochemistry, Vol.43, No.3, 1198-1206, 2017.

(要約): IL-27 might control leukocyte migration in periodontal lesion by modulating chemokine production from epithelial cells.
(徳島大学機関リポジトリ): ● Metadata: 112954
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000481760
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28977795; ● Search Scopus @ Elsevier (PMID): 28977795; ● Search Scopus @ Elsevier (DOI): 10.1159/000481760

(徳島大学機関リポジトリ: 112954, DOI: 10.1159/000481760, PubMed: 28977795) Susilowati Heni, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Kouji Hirao, Katsuhiko Hirota, Takashi Matsuo and Yoichiro Miyake :
Royal jelly inhibits Pseudomonas aeruginosa adherence and reduces excessive inflammatory responses in human epithelial cells,
BioMed Research International, Vol.2017, 3191752, 2017.

(要約): is a Gram-negative bacterium and causes respiratory infection especially in elderly patients. Royal jelly has been used worldwide as a traditional remedy and as a nutrient; however, the effect against is unclear. The aim of this study was to analyze antibacterial, antiadherent, and anti-inflammatory effects of royal jelly against . Wild-type strain PAO1 and clinical isolates of were used for antibacterial assay and antiadherent assay to abiotic surface and epithelial cells, which are pharynx (Detroit 562) and lung (NCI-H292) epithelial cells. In anti-inflammatory assay, epithelial cells were pretreated with royal jelly before bacterial exposure to investigate its inhibitory effect on interleukin (IL-8) and macrophage inflammatory protein-3/CCL20 overproduction. Although royal jelly did not have antibacterial activity at concentration of 50% w/v, antiadherent activity was confirmed on the abiotic surface and epithelial cells under concentration of 25%. Pretreatment with royal jelly significantly inhibited overproduction of IL-8 and CCL20 from both cells. These results demonstrated that royal jelly inhibits adherence and protects epithelial cells from excessive inflammatory responses against infection. Our findings suggested that royal jelly may be a useful supplement as complementary and alternative medicine for preventing respiratory infection caused by .
(キーワード): Cell Line / Chemokine CCL20 / Epithelial Cells / Fatty Acids / Gene Expression Regulation / Humans / 炎症 (inflammation) / Interleukin-8 / Pseudomonas Infections / 緑膿菌 (Pseudomonas aeruginosa)
(徳島大学機関リポジトリ): ● Metadata: 112385
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2017/3191752
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29075644; ● Summary page in Scopus @ Elsevier: 2-s2.0-85030761989

(徳島大学機関リポジトリ: 112385, DOI: 10.1155/2017/3191752, PubMed: 29075644, Elsevier: Scopus) Sekita Yasuko, Keiji Murakami, Hiromichi Yumoto, Kouji Hirao, Takashi Amoh, Natsumi Fujiwara, Katsuhiko Hirota, Hideki Fujii, Takashi Matsuo, Yoichiro Miyake and Yoshiki Kashiwada :
Antibiofilm and Anti-Inflammatory Activities of+ Houttuynia cordata Decoction for Oral Care,
Evidence-Based Complementary and Alternative Medicine : eCAM, Vol.2017, 2850947, 2017.

(要約): Dental biofilms that form in the oral cavity play a critical role in the pathogenesis of several infectious oral diseases, including dental caries, periodontal disease, and oral candidiasis. (HC, Saururaceae) is a widely used traditional medicine, for both internal and external application. A decoction of dried HC leaves (dHC) has long been consumed as a health-promoting herbal tea in Japan. We have recently reported that a water solution of HC poultice ethanol extract (wHCP) exerts antimicrobial and antibiofilm effects against several important oral pathogens. It also exhibits anti-inflammatory effects on human keratinocytes. In our current study, we examined the effects of dHC on infectious oral pathogens and inflammation. Our results demonstrated that dHC exerts moderate antimicrobial effects against methicillin-resistant (MRSA) and other oral microorganisms. dHC also exhibited antibiofilm effects against MRSA, (involved in dental plaque formation), and and inhibitory effects on interleukin-8, CCL20, IP-10, and GRO productions by human oral keratinocytes stimulated by lipopolysaccharide (a cause of periodontal disease), without cytotoxic effects. This suggests that dHC exhibits multiple activities in microorganisms and host cells. dHC can be easily prepared and may be effective in preventing infectious oral diseases.
(徳島大学機関リポジトリ): ● Metadata: 112956
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2017/2850947
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29234378; ● Summary page in Scopus @ Elsevier: 2-s2.0-85042323475

(徳島大学機関リポジトリ: 112956, DOI: 10.1155/2017/2850947, PubMed: 29234378, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
IL-29 Enhances CXCL10 Production in TNF--stimulated Human Oral Epithelial Cells.,
Immunological Investigations, Vol.46, No.6, 615-624, 2017.

(要約): Interleukin-29 (IL-29) is a cytokine belonging to the Type III interferon family. It was recently detected in the gingival crevicular fluid of periodontitis patients. However, the role of IL-29 in the pathogenesis of periodontal disease remains unknown. The aim of this study was to examine the effects of IL-29 on C-X-C motif chemokine ligand 10 (CXCL10) production in human oral epithelial cells. We measured CXCL10 production in TR146 cells, which is a human oral epithelial cell line, using an enzyme-linked immunosorbent assay. We used a Western blot analysis to detect IL-29 receptor expression and the phosphorylation levels of signal transduction molecules, including p38 mitogen-activated protein kinases (MAPK), signal transducer and activator of transcription 3 (STAT3), and nuclear factor (NF)- B p65, in the TR146 cells. The TR146 cells expressed the IL-29 receptor. IL-29 induced CXCL10 production in the TR146 cells. IL-29 significantly enhanced CXCL10 production in tumor necrosis factor (TNF)--stimulated TR146 cells. The p38 MAPK, STAT3, and NF-B pathways were found to be related to the IL-29-induced enhancement of CXCL10 production in TNF--stimulated TR146 cells. IL-29 promotes T helper 1-cell accumulation in periodontal lesions by inducing CXCL10 production in oral epithelial cells.
(徳島大学機関リポジトリ): ● Metadata: 112817
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/08820139.2017.1336176
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28753407; ● Search Scopus @ Elsevier (PMID): 28753407; ● Search Scopus @ Elsevier (DOI): 10.1080/08820139.2017.1336176

(徳島大学機関リポジトリ: 112817, DOI: 10.1080/08820139.2017.1336176, PubMed: 28753407) Natsumi Fujiwara, Keiji Murakami, Michiyasu Nakao, Munehisa Toguchi, Hiromichi Yumoto, Katsuhiko Hirota, Takashi Matsuo, Shigeki Sano, Kazumi Ozaki and Yoichiro Miyake :
Antibacterial and antibiofilm effects of Reuterin-Related Compounds to periodontopathic bacteria,
Journal of Oral Health and Biosciences, Vol.30, No.1, 8-17, 2017.

(徳島大学機関リポジトリ): ● Metadata: 111063
(出版サイトへのリンク): ● Publication site (DOI): 10.20738/johb.30.1_8
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.20738/johb.30.1_8

(徳島大学機関リポジトリ: 111063, DOI: 10.20738/johb.30.1_8) Natsumi Fujiwara, Keiji Murakami, Michiyasu Nakao, Munehisa Toguchi, Hiromichi Yumoto, Takashi Amoh, Katsuhiko Hirota, Takashi Matsuo, Shigeki Sano, Kazumi Ozaki and Yoichiro Miyake :
Novel reuterin-related compounds suppress odour by periodontopathic bacteria,
Oral Diseases, Vol.23, No.4, 492-497, 2017.

(要約): Halitosis is caused by volatile sulphur compounds including methyl mercaptan (CH3 SH) in the oral cavity and is a serious problem that limits interpersonal social communication. The aim of study was to evaluate the effects of reuterin-related compounds (RRCs) on halitosis-related periodontopathic bacteria in vitro. RRC-01, RRC-02 and RRC-03 (32 and 64 g ml(-1) ) in culture media containing Fusobacterium nucleatum JCM8523 and Porphyromonas gingivalis ATCC33277 were used. The effects of RRCs on CH3 SH production and detectable odour by F. nucleatum and P. gingivalis were examined by CH3 SH production assay and organoleptic test, respectively. The number of bacterial cells was also measured using an ATP assay. In P. gingivalis treated with RRCs, the expression of mgl gene, which is responsible for CH3 SH production, was examined by qRT-PCR. CH3 SH production and the score of detectable odour from F. nucleatum and P. gingivalis culture media containing RRCs were significantly lower than that without RRCs (P < 0.05). The expression of mgl gene in P. gingivalis was significantly downregulated by RRC-01 (P < 0.01), but not by RRC-02 or RRC-03. RRCs are potent oral care products for preventing halitosis via reducing CH3 SH production.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/odi.12638
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28083982; ● Summary page in Scopus @ Elsevier: 2-s2.0-85015084211

(DOI: 10.1111/odi.12638, PubMed: 28083982, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
Gomisin N Decreases Inflammatory Cytokine Production in Human Periodontal Ligament Cells.,
Inflammation, Vol.40, No.2, 360-365, 2017.

(要約): Gomisin N, which is a lignan isolated from Schisandra chinensis, has some pharmacological effects. However, the anti-inflammatory effects of gomisin N on periodontal disease are uncertain. The aim of this study was to examine the effect of gomisin N on inflammatory mediator production in tumor necrosis factor (TNF)--stimulated human periodontal ligament cells (HPDLC). Gomisin N inhibited interleukin (IL)-6, IL-8, CC chemokine ligand (CCL) 2, and CCL20 production in TNF--stimulated HPDLC in a dose-dependent manner. Moreover, we revealed that gomisin N could suppress extracellular signal-regulated kinase (ERK) and c-Jun N terminal kinase (JNK) phosphorylation in TNF--stimulated HPDLC though protein kinase B (Akt) phosphorylation was not suppressed by gomisin N treatment. In summary, gomisin N might exert anti-inflammatory effects by attenuating cytokine production in periodontal ligament cells via inhibiting the TNF--stimulated ERK and JNK pathways activation.
(キーワード): Anti-Inflammatory Agents / Cells, Cultured / Cyclooctanes / Cytokines / Dose-Response Relationship, Drug / Humans / Inflammation Mediators / Lignans / MAP Kinase Signaling System / Periodontal Ligament / Phosphorylation / Polycyclic Compounds / Tumor Necrosis Factor-alpha
(徳島大学機関リポジトリ): ● Metadata: 112816
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-016-0482-4
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27896541; ● Search Scopus @ Elsevier (PMID): 27896541; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-016-0482-4

(徳島大学機関リポジトリ: 112816, DOI: 10.1007/s10753-016-0482-4, PubMed: 27896541) Keiji Murakami, Hiromichi Yumoto, Ayu Murakami, Takashi Amoh, Darija Viducic, Katsuhiko Hirota, Atsushi Tabata, Hideaki Nagamune, Hiroki Kourai, Takashi Matsuo and Yoichiro Miyake :
Evaluation of the effectiveness of the potent bis-quaternary ammonium compound, 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) on Pseudomonas aeruginosa.,
Journal of Applied Microbiology, Vol.122, No.4, 893-899, 2017.

(要約): Quaternary ammonium compounds (QACs), including benzalkonium chloride (BAC) and cetylpyridinium chloride (CPC) are cationic surfactants and have been used widely as general disinfectants in the medical field due to their strong antibacterial effects and low cytotoxicity to human cells. 4,4'-( , -hexametylenedithio) bis (1-octylpyridinium bromide) (4DTBP-6,8) is one of the potent bis-QACs synthesized to improve the antimicrobial activities of mono-QACs such as BAC. This study aimed to assess the effectiveness of 4DTBP-6,8 against Pseudomonas aeruginosa, a prevalent hospital pathogen. The minimum inhibitory concentrations of 4DTBP-6,8, CPC and BAC against P. aeruginosa were measured. 4DTBP-6,8 exhibited strong antibacterial activity. We assessed the bactericidal effects of QACs against P. aeruginosa under certain conditions and their cytotoxicities in human epithelial cells using lactate dehydrogenase (LDH) release. 4DTBP-6,8 exerted excellent bactericidal effects against high concentrations of bacteria, biofilm cells and even in the presence of contaminated proteins. Cellular LDH was not released by the treatment with 4DTBP-6,8. 4DTBP-6,8 exhibited the strongest bactericidal activity against P. aeruginosa among the three QACs tested without any cytotoxicity. The potent bis-QAC, 4DTBP-6,8 has the potential to be an effective disinfectant in preventing hospital infections caused by P. aeruginosa.
(キーワード): Anti-Bacterial Agents / Biofilms / Cell Line / Disinfectants / Humans / Niacinamide / 緑膿菌 (Pseudomonas aeruginosa) / Pyridinium Compounds
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.13392
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28035713; ● Summary page in Scopus @ Elsevier: 2-s2.0-85013236835

(DOI: 10.1111/jam.13392, PubMed: 28035713, Elsevier: Scopus) Katsuhiko Hirota, Hiromichi Yumoto, B Sapaar, Takashi Matsuo, Tetsuo Ichikawa and Yoichiro Miyake :
Pathogenic factors in Candida biofilm-related infectious diseases.,
Journal of Applied Microbiology, Vol.122, No.2, 321-330, 2016.

(要約): Candida albicans is a commonly found member of the human microflora and is a major human opportunistic fungal pathogen. A perturbation of the microbiome can lead to infectious diseases caused by various micro-organisms, including C. albicans. Moreover, the interactions between C. albicans and bacteria are considered to play critical roles in human health. The major biological feature of C. albicans, which impacts human health, resides in its ability to form biofilms. In particular, the extracellular matrix (ECM) of Candida biofilm plays a multifaceted role and therefore may be considered as a highly attractive target to combat biofilm-related infectious diseases. In addition, extracellular DNA (eDNA) also plays a crucial role in Candida biofilm formation and its structural integrity and induces the morphological transition from yeast to the hyphal growth form during C. albicans biofilm development. This review focuses on pathogenic factors such as eDNA in Candida biofilm formation and its ECM production and provides meaningful information for future studies to develop a novel strategy to battle infectious diseases elicited by Candida-formed biofilm.
(キーワード): Biofilms / Candida / Candida albicans / Candidiasis / DNA (DNA) / Humans / Hyphae / クォーラムセンシング (quorum sensing) / Respiratory Tract Infections / Virulence Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.13330
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27770500; ● Summary page in Scopus @ Elsevier: 2-s2.0-85005943779

(DOI: 10.1111/jam.13330, PubMed: 27770500, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Yoshihiro Ohta, Kazumi Ozaki and Takashi Matsuo :
Alkannin inhibits CCL3 and CCL5 production in human periodontal ligament cells.,
Cell Biology International, Vol.40, No.12, 1380-1385, 2016.

(要約): Alkannin, which is found in Alkanna tinctoria, a member of the borage family, is used as a food coloring. Alkannin has recently been reported to have certain biological functions, such as anti-microbial and anti-oxidant effects. It is known that CC chemokine receptor (CCR) 5-positive leukocytes contribute to alveolar bone resorption in periodontal lesions. The aim of this study was to examine whether alkannin inhibits the production of CC chemokine ligand (CCL) 3 and CCL5, which are CCR5 ligands, in human periodontal ligament cells (HPDLC). Interleukin (IL)-1 induced CCL3 and CCL5 production in HPDLC. Alkannin inhibited IL-1-mediated CCL3 and CCL5 production in HPDLC in a dose-dependent manner. Moreover, we revealed that alkannin suppressed inhibitor of kappa B- degradation in IL-1-stimulated HPDLC. In addition, a nuclear factor (NF)-B inhibitor significantly inhibited CCL3 and CCL5 production in IL-1-stimulated HPDLC. These results demonstrate that alkannin inhibits CCR5 ligand production in IL-1-stimulated HPDLC by attenuating the NF-B signaling pathway.
(キーワード): Cells, Cultured / Chemokine CCL3 / Chemokine CCL5 / Humans / I-kappa B Proteins / Interleukin-1beta / Ligands / Naphthoquinones / Periodontal Ligament / リン酸化 (phosphorylation) / Proteolysis / Transcription Factor RelA
(徳島大学機関リポジトリ): ● Metadata: 112812
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/cbin.10692
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27743458; ● Search Scopus @ Elsevier (PMID): 27743458; ● Search Scopus @ Elsevier (DOI): 10.1002/cbin.10692

(徳島大学機関リポジトリ: 112812, DOI: 10.1002/cbin.10692, PubMed: 27743458) Yuki Hosokawa, Kouji Hirao, Hiromichi Yumoto, Ayako Washio, Tadashi Nakanishi, Daisuke Takegawa, Chiaki Kitamura and Takashi Matsuo :
Functional roles of NOD1 in odontoblasts on dental pulp innate immunity,
BioMed Research International, Vol.2016, 9325436, 2016.

(要約): Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA. The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay. In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand. CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors. Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells. These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.
(キーワード): Animals / Cell Line / 細胞質分裂 (cytokinesis) / Dental Pulp / 自然免疫 (innate immunity) / Inflammation Mediators / Nod1 Signaling Adaptor Protein / Odontoblasts / Rats
(徳島大学機関リポジトリ): ● Metadata: 112386
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2016/9325436
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27747243; ● Summary page in Scopus @ Elsevier: 2-s2.0-84991669747

(徳島大学機関リポジトリ: 112386, DOI: 10.1155/2016/9325436, PubMed: 27747243, Elsevier: Scopus) Ikuko Hosokawa, Yoshitaka Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
Melatonin Inhibits CXCL10 and MMP-1 Production in IL-1-Stimulated Human Periodontal Ligament Cells.,
Inflammation, Vol.39, No.4, 1520-1526, 2016.

(要約): Melatonin is a hormone that is mainly secreted by the pineal gland and exhibits a wide spectrum of activities, including antioxidant functions. Melatonin has been detected in gingival crevicular fluid. However, the role of melatonin in periodontal tissue is still uncertain. The aim of this study was to examine the effects of melatonin on inflammatory mediator expression in human periodontal ligament cells (HPDLC). Interleukin (IL)-1 induced CXC chemokine ligand (CXCL)10, matrix metalloproteinase (MMP)-1, and tissue inhibitors of metalloproteinase (TIMP)-1 production in HPDLC. Melatonin decreased CXCL10 and MMP-1 production and increased TIMP-1 production in IL-1-stimulated HPDLC. Western blot analysis showed that melatonin inhibited p38 mitogen-activated protein kinase (MAPK) and c-jun N-terminal kinase (JNK) phosphorylation, and IkB- degradation and phosphorylation in IL-1-stimulated HPDLC. These results suggest that melatonin might inhibit Th1 cell migration by reducing CXCL10 production. Moreover, melatonin might inhibit soft tissue destruction by decreasing MMP-1 production in periodontal lesions.
(徳島大学機関リポジトリ): ● Metadata: 112815
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-016-0386-3
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27271323; ● Search Scopus @ Elsevier (PMID): 27271323; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-016-0386-3

(徳島大学機関リポジトリ: 112815, DOI: 10.1007/s10753-016-0386-3, PubMed: 27271323) Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Takashi Amoh, Natsumi Fujiwara, Shohei Ogata, Takashi Matsuo, Yoichiro Miyake and Yoshiki Kashiwada :
Preventive Effects of Houttuynia cordata Extract for Oral Infectious Diseases.,
BioMed Research International, Vol.2016, 2581876, 2016.

(要約): Houttuynia cordata (HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey showed that HC poultice (HCP) prepared from smothering fresh leaves of HC had been frequently used for the treatment of purulent skin diseases with high effectiveness. Our experimental study also demonstrated that ethanol extract of HCP (eHCP) has antibacterial, antibiofilm, and anti-inflammatory effects against S. aureus which caused purulent skin diseases. In this study, we focused on novel effects of HCP against oral infectious diseases, such as periodontal disease and dental caries. We determined the antimicrobial and antibiofilm effects of water solution of HCP ethanol extract (wHCP) against important oral pathogens and investigated its cytotoxicity and anti-inflammatory effects on human oral epithelial cells. wHCP had moderate antimicrobial effects against some oral microorganisms and profound antibiofilm effects against Fusobacterium nucleatum, Streptococcus mutans, and Candida albicans. In addition, wHCP had no cytotoxic effects and could inhibit interleukin-8 and CCL20 productions by Porphyromonas gingivalis lipopolysaccharide-stimulated human oral keratinocytes. Our findings suggested that wHCP may be clinically useful for preventing oral infectious diseases as a mouthwash for oral care.
(キーワード): Anti-Bacterial Agents / 細菌 (bacteria) / Biofilms / Cell Line / Cell Survival / Dental Caries / Houttuynia / Humans / Periodontal Diseases / Plant Extracts
(徳島大学機関リポジトリ): ● Metadata: 112384
(出版サイトへのリンク): ● Publication site (DOI): 10.1155/2016/2581876
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27413739; ● Summary page in Scopus @ Elsevier: 2-s2.0-84978765486

(徳島大学機関リポジトリ: 112384, DOI: 10.1155/2016/2581876, PubMed: 27413739, Elsevier: Scopus) Satoru Shindo, Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Shikonin Inhibits Inflammatory Cytokine Production in Human Periodontal Ligament Cells.,
Inflammation, Vol.39, No.3, 1124-1129, 2016.

(要約): Shikonin, which is derived from Lithospermum erythrorhizon, a herb used in traditional medicine, has long been considered to be a useful treatment for various diseases in traditional oriental medicine. Shikonin has recently been reported to have several pharmacological properties, e.g., it has anti-microbial, anti-tumor, and anti-inflammatory effects. The aim of this study was to examine whether shikonin is able to influence the production of interleukin (IL)-6, IL-8, and/or chemokine C-C motif ligand (CCL)20, which contribute to the pathogenesis of periodontal disease, in human periodontal ligament cells (HPDLC). The production levels of IL-6, IL-8, and CCL20 in HPDLC were determined using an ELISA. Western blot analysis was used to detect nuclear factor kappa B (NF-B) pathway activation in HPDLC. Shikonin prevented IL-1- or tumor necrosis factor (TNF)--mediated IL-6, IL-8, and CCL20 production in HPDLC. Moreover, we found that shikonin suppressed the phosphorylation and degradation of inhibitor of kappa B-alpha (IB-) in IL-1- or TNF--stimulated HPDLC. These findings suggest that shikonin could have direct beneficial effects against periodontal disease by reducing IL-6, IL-8, and CCL20 production in periodontal lesions.
(徳島大学機関リポジトリ): ● Metadata: 112814
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-016-0344-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27072015; ● Search Scopus @ Elsevier (PMID): 27072015; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-016-0344-0

(徳島大学機関リポジトリ: 112814, DOI: 10.1007/s10753-016-0344-0, PubMed: 27072015) Yasuko Sekita, Keiji Murakami, Hiromichi Yumoto, Hiroyuki Mizuguchi, Takashi Amoh, Satoshi Ogino, Takashi Matsuo, Yoichiro Miyake, Hiroyuki Fukui and Yoshiki Kashiwada :
Anti-bacterial and anti-inflammatory effects of ethanol extract from Houttuynia cordata poultice.,
Bioscience, Biotechnology, and Biochemistry, 2016.

(要約): Houttuynia cordata (HC) has been commonly used as many traditional remedies in local areas of Japan. Although many pharmacological activities of HC have been reported, the mechanism underlying the effect of HC remains unknown. We conducted the interview survey in Japan to verify how HC was actually used. The interview survey revealed that HC poultice (HCP) prepared from smothering fresh leaves of HC was most frequently used for the treatment of purulent skin diseases including furuncle and carbuncle with high effectiveness. Ethanol extract of HCP (eHCP) showed anti-bacterial effects against methicillin-resistant Staphylococcus aureus (MRSA), and showed an anti-biofilm activity against MRSA. eHCP showed dose-dependent inhibition of S. aureus lipoteichoic acid (LTA)-induced interleukin-8 and CCL20 production in human keratinocyte without any cytotoxicity. These results suggest that HCP is effective for skin abscess and its underlying mechanism might be the complicated multiple activities for both bacteria and host cells.
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09168451.2016.1151339
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27023331; ● Search Scopus @ Elsevier (PMID): 27023331; ● Search Scopus @ Elsevier (DOI): 10.1080/09168451.2016.1151339

(DOI: 10.1080/09168451.2016.1151339, PubMed: 27023331) 山村佳子, 滝沢宏光, 松本文博, 桃田幸弘, 青田桂子, 武川大輔, 近藤智香, 山ノ井朋子, 高野栄之, 可児耕一, 十川悠香, 河野文昭, 松尾敬志, 先山正二, 東雅之 :
胸腔鏡下肺葉切除術における周術期口腔機能管理の効果に関する検討―後ろ向き研究―,
日本口腔ケア学会雑誌, Vol.10, No.1, 106-110, 2016年.

(キーワード): 胸腔鏡下肺葉切除術 / 周術期口腔機能管理 / 術後合併症 / thoracoscopic lung lobectomy / Perioperative oral management / postoperative complications
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1522262181026542720

(CiNii: 1522262181026542720) Tsuyoshi Miyagawa, Tsuyoshi Fujita, Hiromichi Yumoto, Tetsuya Yoshimoto, Mikihito Kajiya, Kazuhisa Ouhara, Shinji Matsuda, Hideki Shiba, Takashi Matsuo and Hidemi Kurihara :
Azithromycin recovers reductions in barrier function in human gingival epithelial cells stimulated with tumor necrosis factor-α,
Archives of Oral Biology, Vol.62, 64-69, 2016.

(要約): The gingival epithelium plays an important role in protecting against the invasion of periodontal pathogens, and the permeability of gingival epithelial cells has been implicated in the initiation of periodontitis. Azithromycin (AZM) has been used in the treatment of chronic inflammatory airway diseases because it regulates cell-cell contact in airway epithelial cells. Therefore, AZM may also regulate barrier function in gingival epithelial cells. In the present study, we examined the effects of AZM on the permeability of human gingival epithelial cells (HGEC) under inflammatory conditions in vitro. HGEC were stimulated by tumor necrosis factor-α (TNF-α) in the presence of AZM or p38 MAP kinase and ERK inhibitors. Permeability was assessed based on transepithelial electrical resistance (TER). The expression of E-cadherin, phosphorylated p38 MAP kinase, and ERK was analyzed by Western blotting. TNF-α decreased TER in HGEC, and AZM and the p38 MAP kinase and ERK inhibitors recovered this decrease. AZM inhibited the phosphorylation of ERK and p38 MAP kinase in TNF-α-stimulated HGEC. Furthermore, AZM recovered the decrease in E-cadherin expression in HGEC stimulated with TNF-α. These results suggested that AZM regulated gingival epithelial permeability through p38 MAP kinase and ERK signaling, and may contribute to suppress the inflammation in gingival tissue.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.archoralbio.2015.11.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26655749; ● Summary page in Scopus @ Elsevier: 2-s2.0-84949193967

(DOI: 10.1016/j.archoralbio.2015.11.015, PubMed: 26655749, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
IL-4 Modulates CCL11 and CCL20 Productions from IL-1-Stimulated Human Periodontal Ligament Cells.,
Cellular Physiology and Biochemistry, Vol.38, No.1, 153-159, 2016.

(要約): These results mean that IL-4 enhanced Th2 cells migration in periodontal lesion to induce CCL11 production from HPDLCs. On the other hand, IL-4 inhibits Th17 cells accumulation in periodontally diseased tissues to inhibit CCL20 production. Therefore, IL-4 is positively related with the pathogenesis of periodontal disease to control chemokine productions in periodontal lesions.
(徳島大学機関リポジトリ): ● Metadata: 112953
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000438617
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26765337; ● Search Scopus @ Elsevier (PMID): 26765337; ● Search Scopus @ Elsevier (DOI): 10.1159/000438617

(徳島大学機関リポジトリ: 112953, DOI: 10.1159/000438617, PubMed: 26765337) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
Calcitriol Suppressed Inflammatory Reactions in IL-1-Stimulated Human Periodontal Ligament Cells.,
Inflammation, Vol.38, No.6, 2252-2258, 2015.

(要約): Vitamin D has important roles on control of calcium and phosphate levels in the body. However, the role of vitamin D on the pathogenesis of periodontal disease is still uncertain. Therefore, we examined the effect of the hormonal form of vitamin D, calcitriol, on inflammatory responses of human periodontal ligament cells (HPDLC). We detected vitamin D receptor expression in non-stimulated HPDLC. Calcitriol inhibited interleukin (IL)-6, IL-8, CC chemokine ligand (CCL) 20, CXC chemokine ligand (CXCL) 10, and matrix metalloproteinase (MMP)-3 release from IL-1-stimulated HPDLC. Tissue inhibitor of metalloproteinase (TIMP)-1 production did not change by calcitriol. Moreover, we found c-jun N-terminal kinase (JNK) phosphorylation and IB- degradation in IL-1-stimulated HPDLC were inhibited by calcitriol, and JNK and nuclear factor (NF)-B inhibitors could decrease IL-6, IL-8, CCL20, CXCL10, and MMP-3 productions in IL-1-treated HPDLC. These findings suggest that vitamin D could modulate inflammatory response in periodontal tissues.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-015-0209-y
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 26156812; ● Summary page in Scopus @ Elsevier: 2-s2.0-84946497259

(DOI: 10.1007/s10753-015-0209-y, PubMed: 26156812, Elsevier: Scopus) Tadashi Nakanishi, Kayo Mukai, Yoshitaka Hosokawa, Daisuke Takegawa and Takashi Matsuo :
Catechins inhibit vascular endothelial growth factor production and cyclooxygenase-2 expression in human dental pulp cells,
International Endodontic Journal, Vol.48, No.3, 277-282, 2015.

(要約): To investigate the effect of catechins on vascular endothelial growth factor (VEGF) production and cyclooxygenase-2 (COX-2) expression in human dental pulp cells (HDPC) stimulated with bacteria-derived factors or pro-inflammatory cytokines. Morphologically fibroblastic cells established from explant cultures of healthy human dental pulp tissues were used as HDPC. HDPC pre-treated with catechins, epigallocatechin-3-gallate (EGCG) or epicatechin gallate (ECG), were exposed to lipopolysaccharide (LPS), peptidoglycan (PG), interlukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α). VEGF production was examined by enzyme-linked immunosorbent assay, and COX-2 expression was assessed by immunoblot. EGCG and ECG significantly reduced LPS- or PG-mediated VEGF production in the HDPC in a dose-dependent manner. EGCG also prevented IL-1β-mediated VEGF production. Although TNF-α did not enhance VEGF production in the dental pulp cells, treatment of 20 μg mL(-1) of EGCG decreased the level of VEGF. In addition, the catechins attenuated COX-2 expression induced by LPS and IL-1β. The up-regulated VEGF and COX-2 expressions in the HDPC stimulated with these bacteria-derived factors or IL-1β were diminished by the treatment of EGCG and ECG. These findings suggest that the catechins may be beneficial as an anti-inflammatory tool of the treatment for pulpal inflammation.
(キーワード): catechin / cyclooxygenase-2 / dental pulp / vascular endothelial growth factor
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/iej.12312
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24847951; ● Search Scopus @ Elsevier (PMID): 24847951; ● Search Scopus @ Elsevier (DOI): 10.1111/iej.12312

(DOI: 10.1111/iej.12312, PubMed: 24847951) 吉岡昌美, 永井浩美, 石川久子, 東雅之, 河野文昭, 松尾敬志 :
徳島県下の地域歯科診療所における病院・施設への往診/訪問歯科診療の現状,
四国公衆衛生学会雑誌, Vol.60, No.1, 103-107, 2015年. Hiromichi Yumoto, Kouji Hirao, Toshihiko Tominaga, Naoki Bando, Kanako Takahashi and Takashi Matsuo :
Electromagnetic wave irradiation promotes osteoblastic cell proliferation and up-regulates growth factors via activation of the ERK1/2 and p38 MAPK pathways,
Cellular Physiology and Biochemistry, Vol.35, No.2, 601-615, 2015.

(要約): Periodontitis with bone resorption is caused by inflammatory reactions to bacterial infection. We recently reported that electromagnetic wave irradiation (EMWI) has bactericidal effects. However, the effects of EMWI on periodontal tissues remain unclear. This study was aimed to investigate the effects of EMWI on osteoblasts. Osteoblastic cells MC3T3-E1 were treated with EMWI (500-1,000 kHz, 5 times, 1 sec/time). Cell growth and cytotoxicity were determined by cell proliferation assays and measurement of lactate dehydrogenase release, respectively. Gene expression and protein production of growth factors were analyzed using real-time PCR and ELISA, respectively. EMWI-activated cellular signal transduction pathways were investigated by immunoblotting and blocking assay with specific inhibitors. Osteoblasts proliferation was significantly enhanced 3 days after EMWI and no cytotoxicity was observed. EMWI up-regulated various growth factors, such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). EMWI induced ERK1/2, p38 MAPK and SAPK/JNK phosphorylation within 5 min, and the production of PDGF-ΑΑ and VEGF was partially reduced by MAPK-specific inhibitor. These findings demonstrated that EMWI increases osteoblastic cell activity and the expression of growth factors via ERK1/2 and p38 MAPK pathways and suggested that EMWI may be beneficial to bone tissue repair such as periodontitis.
(キーワード): Animals / アポトーシス (apoptosis) / Cell Line / Cell Proliferation / Electromagnetic Radiation / MAP Kinase Signaling System / Mice / Mice, Inbred C57BL / Osteoblasts / リン酸化 (phosphorylation) / Platelet-Derived Growth Factor / Up-Regulation / Vascular Endothelial Growth Factor A
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000369722
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25612851; ● Summary page in Scopus @ Elsevier: 2-s2.0-84921876910

(DOI: 10.1159/000369722, PubMed: 25612851, Elsevier: Scopus) 青田桂子, 山村佳子, 山ノ井朋子, 武川大輔, 可児耕一, 高野栄之, 桃田幸弘, 松本文博, 菅原千恵子, 吉岡昌美, 河野文昭, 松尾敬志, 東雅之 :
徳島大学病院における周術期口腔機能管理の現状と課題,
Journal of Oral Health and Biosciences, Vol.28, No.1, 29-36, 2015年.

(要約): It has been shown that oral hygiene affects the onset of perioperative complications. The usefulness of perioperative oral function management aiming at the outbreak decrease in treatment complications and an early discharge was recognized. As a result, perioperative oral function management fee was founded at revision of medical service fees in Fiscal year 2012. In this clinical study, we evaluated the implementation of perioperative oral function management in Tokushima University Hospital. We examined 781 patients, including 563 patients for surgery and 218 patients for chemotherapy and radiotherapy. The mean age of patients was 58.8 ± 12.4 years old. The implementation rate of perioperative oral function management was 9.7% in the patients of surgery, and 17.4% in those of chemotherapy and radiotherapy. The highly required medical department was neurosurgery in the patients of surgery, and hematology in those of chemotherapy and radiotherapy. The mean number of tooth present was 21.3 ± 7.1 in the patients of surgery, and 19.8 ± 7.2 in those chemotherapy and radiotherapy. The rate of dental treatment was required in 40.5% of total patients who received surgery, and in 51.4% of patients who received chemotherapy and radiotherapy.The rate of patients who received denture treatment attained to 11.9% of the whole patients receiving surgery, and 13.3% of patients receiving chemotherapy and radiotherapy. It was revealed that there were many patients required potential demands in perioperative oral function management, and that there were many patients who need dental or denture treatment. We would like to develop perioperative oral function management by the interprofessional collaboration in health and social care.7月発行だが，6月号である．
(キーワード): 徳島大学病院 / 周術期口腔機能管理 / チーム医療
(徳島大学機関リポジトリ): ● Metadata: 109866
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050564287418444544

(徳島大学機関リポジトリ: 109866, CiNii: 1050564287418444544) 山村佳子, 青田桂子, 武川大輔, 十川悠香, 近藤智香, 山ノ井朋子, 高野栄之, 可児耕一, 松本文博, 桃田幸弘, 里見淳一郎, 河野文昭, 松尾敬志, 永廣信治, 東雅之 :
徳島大学病院脳卒中センターにおける口腔管理の意義について,
Journal of Oral Health and Biosciences, Vol.9, No.1, 37-41, 2015年. Satoru Shindo, Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Genipin inhibits MMP-1 and MMP-3 release from TNF--stimulated human periodontal ligament cells.,
Biochimie, Vol.107PB, 391-395, 2014.

(要約): Genipin, the aglycon of geniposide found in gardenia fruit has long been considered for treatment of inflammatory diseases in traditional oriental medicine. Genipin has recently been reported to have some pharmacological functions, such as antimicrobial, antitumor, and anti-inflammatory effects. The aim of this study was to examine whether genipin could modify matrix metalloproteinase (MMP)-1 and MMP-3, which are related to the destruction of periodontal tissues in periodontal lesion, expression in tumor necrosis factor (TNF)--stimulated human periodontal ligament cells (HPDLCs). Genipin prevented TNF--mediated MMP-1 and MMP-3 productions in HPDLCs. Moreover, genipin could suppress not only extracellular signal-regulated kinase (ERK) and Jun-N-terminal kinase (JNK) phosphorylations but also AMP-activated protein kinase (AMPK) phosphorylation in TNF--stimulated HPDLCs. Inhibitors of ERK and AMPK could inhibit both MMP-1 and MMP-3 productions. Moreover, we revealed the ERK inhibitor suppressed AMPK phosphorylation in TNF--stimulated HPDLCs. These data provide a new mechanism through which genipin could be used for the treatment of periodontal disease to prevent MMPs expression in periodontal lesion.
(徳島大学機関リポジトリ): ● Metadata: 110103
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.biochi.2014.10.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25457105; ● Summary page in Scopus @ Elsevier: 2-s2.0-84915753375

(徳島大学機関リポジトリ: 110103, DOI: 10.1016/j.biochi.2014.10.008, PubMed: 25457105, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
IL-22 Enhances CCL20 Production in IL-1-Stimulated Human Gingival Fibroblasts.,
Inflammation, Vol.37, No.6, 2062-2066, 2014.

(要約): CC chemokine ligand 20 (CCL20) is involved in the recruitment of Th17 cells and thus in the exacerbation of periodontal disease, but the effect of simultaneous interleukin (IL)-22 and IL-1 stimulation on CCL20 production in human gingival fibroblasts (HGFs) is uncertain. In this study, we investigated the mechanisms of IL-1- and/or IL-22-induced CCL20 production in HGFs. A single stimulation of IL-22 could not induce CCL20 production. On the other hand, IL-22 could increase CCL20 production from IL-1-stimulated HGFs in a dose-dependent manner. C-Jun N terminal kinase (JNK) and inhibitor of nuclear factor B (IB)- phosphorylation were increased in IL-1- and IL-22-stimulated HGFs. An inhibitor of nuclear factor (NF)-B decreased IL-1- and IL-22-induced CCL20 production, though an inhibitor of JNK did not modulate CCL20 production. These data suggest that IL-1 in cooperation with IL-22 could increase Th17 cell accumulation in periodontally diseased tissues to enhance CCL20 production in HGFs.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-014-9939-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24902798; ● Summary page in Scopus @ Elsevier: 2-s2.0-84937557635

(DOI: 10.1007/s10753-014-9939-5, PubMed: 24902798, Elsevier: Scopus) Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Modulatory roles of interferon-γ through indoleamine 2, 3-dioxygenase induction in innate immune response of dental pulp cells.,
Journal of Endodontics, Vol.40, No.9, 1382-1387, 2014.

(要約): Marked infiltration of inflammatory cells such as activated T cells producing interferon-γ (IFN-γ) is observed in severe pulpitis. However, the roles of IFN-γ in the innate immune response of dental pulp have not been reported. Indoleamine 2, 3-dioxygenase (IDO) is a regulator of immune responses, and the IDO expression is induced by IFN-γ in many cells whose expression in dental pulp is unknown. The purpose of this study was to determine the role of IFN-γ in the immune response through microbial pattern recognition receptors (PRRs) such as Toll-like receptors or nucleotide-binding oligomerization domain-like receptors on the production of proinflammatory cytokines such as CXCL10 and interleukin (IL)-6 and the expression of IDO in cultured human dental pulp cells (HDPCs). HDPCs were established from explant cultures of healthy pulp tissues. CXCL10 and IL-6 production was determined using enzyme-linked immunosorbent assay. Confirmation of IDO localization in dental pulp tissues was examined using immunohistochemistry. IDO expression in HDPCs was analyzed by immunoblot. IFN-γ significantly up-regulated CXCL10 and IL-6 production in the HDPCs stimulated with ligands for PRRs in a concentration-dependent manner. The expression of IDO was detected in inflamed pulp tissue. In addition, IFN-γ in combination with the PRR ligands enhanced IDO expression in HDPCs compared with IFN-γ alone. Moreover, CXCL10 production in IFN-γ-stimulated HDPCs was inhibited by an IDO inhibitor. This study showed the synergistic effects by IFN-γ on cytokine production and IDO expression in HDPCs, suggesting that IFN-γ may modulate the innate immune response of dental pulp.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.joen.2014.03.018
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25146019; ● Summary page in Scopus @ Elsevier: 2-s2.0-85027943562

(DOI: 10.1016/j.joen.2014.03.018, PubMed: 25146019, Elsevier: Scopus) Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Tsuyoshi Miyazaki, Nobuyuki Yamamoto, Koji Miyamoto, Keiji Murakami, Natsumi Fujiwara, Takashi Matsuo and Yoichiro Miyake :
Anti-inflammatory and protective effects of 2-methacryloyloxyethyl phosphorylcholine polymer on oral epithelial cells.,
Journal of Biomedical Materials Research. Part A, Vol.103, No.2, 555-563, 2014.

(要約): Periodontitis is a chronic inflammatory disease initiated by a microbial biofilm formed in the periodontal pocket. Gingival epithelium plays important roles as the first physical barrier to bacterial invasion and in orchestrating the innate immune reaction via toll-like receptors (TLRs), which recognize various bacterial products, and maintaining its function. Newly developed oral care products to inhibit bacterial adherence, subsequent inflammatory reaction and protect the gingival epithelium are expected. We previously reported that 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer coating decreased bacterial adhesion to human oral keratinocytes, RT-7, and mouth-rinsing with MPC-polymer inhibited the increase of oral bacteria. In this study, regarding the possibility of MPC-polymer application for preventing the adherence of periodontal pathogen, subsequent inflammatory reaction and protection of gingival epithelium, we examined the effects of MPC-polymer on the adherence of Porphyromonas gingivalis, major periodontitis-related pathogen, and TLR2 ligand to RT-7 and subsequent interleukin (IL)-8 production. MPC-polymer treatment significantly reduced P. gingivalis adherence by 44% and TLR2-mediated IL-8 production by blocking the binding of its specific-ligand in a concentration-dependent manner. Furthermore, MPC-polymer pretreatment protected RT-7 from injury by chemical irritants, cetylpyridinium chloride. These findings suggest that MPC-polymer is potentially useful for oral care to prevent oral infection and to maintain oral epithelial function. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2014.
(キーワード): Anti-Inflammatory Agents / Bacteroidaceae Infections / Cell Line, Transformed / Epithelial Cells / Humans / Mouth / 歯周病 (periodontitis) / Phosphorylcholine / Polymethacrylic Acids / Porphyromonas gingivalis
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.35201
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24753309; ● Summary page in Scopus @ Elsevier: 2-s2.0-84924401882

(DOI: 10.1002/jbm.a.35201, PubMed: 24753309, Elsevier: Scopus) Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa,
Bacterial Adherence & Biofilm, Vol.27, 45-47, 2014. Yoshitaka Hosokawa, Satoru Shindo, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
IL-6 trans-signaling enhances CCL20 production from IL-1-stimulated human periodontal ligament cells.,
Inflammation, Vol.37, No.2, 381-386, 2014.

(要約): CC chemokine ligand 20 (CCL20) plays a central role in the recruitment of CCR6-expressing cells, including Th17 cells which are related to bone resorption in periodontal lesions and thus in the development of periodontal disease. IL-6 is an important cytokine that is associated with the pathogenesis of periodontitis. However, the effect of IL-6 on CCL20 production is uncertain. The aim of this study was to examine whether IL-6 could modify CCL20 expression in human periodontal ligament cells (HPDLCs). HPDLCs expressed gp130 but did not express IL-6R on the surface of HPDLCs. So, IL-6 trans-signaling is important to recognize IL-6 by HPDLCs. IL-6/sIL-6R stimulation enhanced CCL20 production in IL-1-stimulated HPDLCs. IL-6 produced from IL-1-stimulated HPDLCs with sIL-6R could increase CCL20 production in HPDLCs with sIL-6R. Signal transducer and activator of transcription (STAT)3 activation was related to CCL20 production in IL-1 and IL-6/sIL-6R-stimulated HPDLCs. Our data suggests that HPDLCs, in response to IL-6, sIL-6R, and IL-1, may shift chemokine production to that favoring CCR6-expressing cells recruitment in periodontal lesions.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-013-9750-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24081898; ● Summary page in Scopus @ Elsevier: 2-s2.0-84896326562

(DOI: 10.1007/s10753-013-9750-8, PubMed: 24081898, Elsevier: Scopus) B Sapaar, A Nur, Katsuhiko Hirota, Hiromichi Yumoto, Keiji Murakami, T Amoh, Takashi Matsuo, Tetsuo Ichikawa and Yoichiro Miyake :
Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation.,
Journal of Applied Microbiology, Vol.116, No.6, 1531-1542, 2014.

(要約): The aim of this study was to investigate the effects of genomic DNA purified from Candida albicans and pneumonia-related pathogens, Pseudomonas aeruginosa and Staphylococcus aureus, on in vitro biofilm formation and morphological change of 3 Candida species (C. albicans, C. glabrata, and C. tropicalis). Biofilm formation was evaluated by the crystal violet assay and colony-forming unit counts. Morphological characteristics of biofilms were evaluated by scanning electron microscopy and fluorescence microscopy. Addition of DNA at a low concentration (<1·0 μg ml(-1)) significantly increased biofilm mass of all three Candida species. In contrast, the addition of DNA at a high concentration (10 μg ml(-1)) decreased the biofilm mass. Interestingly, the formation of hyphae in a dense network of yeast cells was observed in C. albicans biofilms exposed to a low concentration of DNA (<1·0 μg ml(-1)). These findings demonstrated that extracellular DNA (eDNA) plays a crucial role in Candida biofilm formation and suggested that eDNA may induce the morphological transition from yeast to hyphal growth form during C. albicans biofilm development. A novel therapy targeting eDNA may be applicable for Candida infection to decrease biofilm formation and hyphal formation.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.12483
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24661775; ● Summary page in Scopus @ Elsevier: 2-s2.0-84900806168

(DOI: 10.1111/jam.12483, PubMed: 24661775, Elsevier: Scopus) Satoru Shindo, Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
Genipin inhibits IL-1-induced CCL20 and IL-6 production from human periodontal ligament cells.,
Cellular Physiology and Biochemistry, Vol.33, No.2, 357-364, 2014.

(要約): These data provide a novel mechanism through which genipin could be used to provide direct benefits in periodontal disease to inhibit IL-6 and CCL20 productions in periodontal lesions.
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000356675
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24557363; ● Summary page in Scopus @ Elsevier: 2-s2.0-84895641086

(DOI: 10.1159/000356675, PubMed: 24557363, Elsevier: Scopus) Wang Yiwei, 湯本浩通, Liu Dali, 松尾敬志, Shu Rong :
The comparison of the ability of polysaccharide from different Porphyromonas gingivalis in inducing THP-1 cells to produce cytokines,
Chinese Journal of Stomatology, Vol.49, No.2, 78-83, 2014年.

(要約): To compare the ability of the polysaccharide from various Porphyromonas gingivalis (Pg) type and clinical strains in inducing THP-1 cells to produce cytokines interleukin(IL)-1β, IL-8, and tumor necrosis factor(TNF)-α, in order to analyze the immunogenicity of Pg polysaccharide components and the virulence-associated factors of this periodontal pathogen. The bacterial polysaccharide was extracted from high virulent Pg strains, W83, SJD2, SJD12 and low virulent Pg, ATCC33277, SJD4, SJD5, and SJD11 by phenol-water extraction. The extracted polysaccharide was used to stimulate the THP-1 cells with different simulation periods and doses. The level of the cytokines, including IL-1β,IL-8 and TNF-α in the cell culture suspension was measured by enzyme-linked immunosorbent assay(ELISA). The polysaccharide extraction of Pg strains was composed of lipopolysaccharide(LPS) and capsular polysaccharide. The secretion of IL-1β, IL-8 and TNF-α, produced by the THP-1 cells showed in a time- and dose-dependent manner in the medium containing 10% fetal bovine serum. The level of these cytokines of the high virulent strains was higher than that of the low virulent strains in medium containing 1% fetal bovine serum.Four hours after stimulation with polysaccharide extracted from high virulent strains, the levels of IL-1β,IL-8, and TNF-α in the cell suspension were (1 639 ± 497), (1 648 ± 513) and (140 ± 48) µg/L, respectively, whereas for low virulent strains, the levels of IL-1β, IL-8, and TNF-α were (773 ± 382), (892 ± 400) and (67 ± 33) µg/L, respectively. Polysaccharide extracted from Pg could induced the THP-1 cells to secrete the cytokines of IL-1β, IL-8 and TNF-α. The level of the cytokines produced by the THP-1 cells associates with the bacterial virulent properties.
(キーワード): Cytokines / Interleukin-1beta / Interleukin-8 / Lipopolysaccharides / Porphyromonas gingivalis / Tumor Necrosis Factor-alpha
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24731418; ● Summary page in Scopus @ Elsevier: 2-s2.0-84929938924

(PubMed: 24731418, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
(-)-Epigallocatechin-3-Gallate Inhibits CC Chemokine Ligand 11 Production in Human Gingival Fibroblasts.,
Cellular Physiology and Biochemistry, Vol.31, No.6, 960-967, 2013.

(要約): Background: CC chemokine ligand 11 (CCL11) is related to Th2 cells migration via CC chemokine receptor 3 (CCR3). Th2 cells are involved in the etiology of periodontal disease. However, how the infiltration of Th2 cells is controlled in periodontally diseased tissues is unknown. (-)-Epigallocatechin gallate (EGCG), the major catechin in green tea, has multiple beneficial effects, but the effects of EGCG on CCL11 production are uncertain. In this study, we investigated whether cytokines could induce CCL11 production in human gingival fibroblasts (HGFs). Moreover, we examined the effects of EGCG on CCL11 production in HGFs. Methods and Results: ELISA analysis disclosed that interleukin (IL)-4 synergistically enhanced CCL11 production in IL-1 or tumor necrosis factor (TNF)--stimulated HGFs. EGCG prevented IL-1/ IL-4 or TNF-/IL-4-mediated CCL11 production in a concentration dependent manner. CCL11 production in HGFs was positively regulated by p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK), and c-Jun N terminal kinase (JNK). Western blot analysis revealed that EGCG treatment prevented IL-1/IL-4 or TNF-/IL-4-induced ERK and JNK activation in HGFs. Conclusions: These data provide that CCL11 production in HGFs could be associated with Th2 cells infiltration in periodontal lesions. Moreover, EGCG is useful for periodontitis treatment to inhibit CCL11 production.
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000350114
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23839108; ● Search Scopus @ Elsevier (PMID): 23839108; ● Search Scopus @ Elsevier (DOI): 10.1159/000350114

(DOI: 10.1159/000350114, PubMed: 23839108) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
TLR3 agonist enhances CC chemokine ligand 20 production in IL-1-stimulated human gingival fibroblasts.,
Cellular Immunology, Vol.283, No.1-2, 8-11, 2013.

(要約): Viruses are related to the etiology of periodontitis. However, the role of viruses on Th17 cells infiltration in periodontitis lesions is unknown. Therefore, we examined the effects of TLR3 ligand on CCL20, which is related to Th17 cells migration, production in human gingival fibroblasts (HGFs). Polyinosinic-polycytidylic acid (Poly I:C), which is a TLR3 agonist, stimulation could moderately induce CCL20 production in HGFs. Poly I:C synergistically enhanced CCL20 expression from IL-1-stimulated HGFs. Inhibitors of p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N terminal kinase (JNK), and NF-B significantly inhibited CCL20 production in Poly I:C/IL-1-stimulated HGFs. Western blot analysis disclosed phosphorylation of p38 MAPK, JNK, and IB- were enhanced in Poly I:C/IL-1-treated HGFs. These data suggested that virus infection is related to Th17 cells migration in periodontitis lesion to induce CCL20 production in HGFs via TLR3. Therefore, our results indicated that virus might be important pathogen in periodontal disease.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cellimm.2013.05.005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23850670; ● Search Scopus @ Elsevier (PMID): 23850670; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cellimm.2013.05.005

(DOI: 10.1016/j.cellimm.2013.05.005, PubMed: 23850670) Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Role of Extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermeius,
Bacterial Adherence & Biofilm, Vol.26, 25-29, 2013. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, D Liu, Kanako Takahashi, Keiji Murakami, Takashi Matsuo, R Shu and Yoichiro Miyake :
Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm.,
Journal of Applied Microbiology, Vol.115, No.1, 260-270, 2013.

(要約): The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA-binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity. Formed biofilm mass was measured with 0·1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml(-1) ) markedly decreased biofilm formation and cell density in biofilms. Colocalization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml(-1) ) purified from Strep. intermedius, other Gram-positive bacteria, Gram-negative bacteria, or human KB cells into the Strep. intermedius culture increased the biofilm mass of all tested strains of Strep. intermedius, wild-type, HLP-downregulated strain and control strains. In contrast, the addition of eDNA (>1 μg ml(-1) ) decreased the biofilm mass of all Strep. intermedius strains. These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity. eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
(キーワード): Biofilms / Cell Line, Tumor / DNA (DNA) / DNA-Binding Proteins / Deoxyribonuclease I / Humans / Streptococcus intermedius
(徳島大学機関リポジトリ): ● Metadata: 106279
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/jam.12202
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23551549; ● Summary page in Scopus @ Elsevier: 2-s2.0-84879085342

(徳島大学機関リポジトリ: 106279, DOI: 10.1111/jam.12202, PubMed: 23551549, Elsevier: Scopus) Hiromichi Yumoto, Toshihiko Tominaga, Kouji Hirao, Tomoko Kimura, Kanako Takahashi, Takashi Sumitomo, Naoki Bando and Takashi Matsuo :
Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation,
Journal of Applied Microbiology, Vol.113, No.1, 181-191, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2672.2012.05307.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22507081; ● Search Scopus @ Elsevier (PMID): 22507081; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2672.2012.05307.x

(DOI: 10.1111/j.1365-2672.2012.05307.x, PubMed: 22507081) Toshiyuki Suge, Ishikawa Kunio and Takashi Matsuo :
Changes in the crystallinity of hydroxyapatite powder and structure of enamel treated with several concentrations of ammonium hexafluorosilicate,
American Journal of Dentistry, Vol.25, No.5, 299-302, 2012.

(要約): To evaluate the effects of changing concentrations of ammonium hexafluorosilicate [SiF: (NH4)2SiF6] solution on the crystallinity of hydroxyapatite powder (HAP) and structure of human enamel in order to overcome the tooth discoloration caused by diamine silver fluoride [AgF: (NH3)2AgF] application. HAP was treated with several concentrations of SiF solution (from 10 to 19,400 ppm) for 5 minutes. The crystallinity of the HAP before and after SiF treatment was then measured by powder X-ray diffraction (XRD) analysis. The angular width (beta) of the 002 diffraction peak was measured at 1/2 the height of the maximum intensity. Also, enamel specimens were prepared from a human extracted tooth. Several concentrations of SiF solution were applied to polished or phosphoric acid etched enamel specimens. The enamel surface was then observed using scanning electron microscopy (SEM). XRD peaks became sharper after SiF treatment indicating that the crystallinity of apatite powder was increased. The 1/beta value was increased from 2.8 +/- 0.1 to 4.3 +/- 0.1 after treatment with 1,000 ppm SiF solution. The amount of CaF2 formed in HAP was gradually increased with increasing concentrations of SiF solution. TheXRD pattern was consistent with CaF2 in case of over 9,000 ppm SiF solution. SEM photographs demonstrated that exposed enamel rods with acid etching were filled with CaF2-like precipitate after SiF treatment regardless of the concentration of SiF solution.
(キーワード): Adult / Crystallization / Dental Enamel / Durapatite / Fluorides / Humans / Powders / Quaternary Ammonium Compounds / Silicic Acid
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23243979; ● Search Scopus @ Elsevier (PMID): 23243979

(PubMed: 23243979) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Tumor necrosis factor-like weak inducer of apoptosis increases CC chemokine ligand 20 production in interleukin 1-stimulated human gingival fibroblasts.,
Human Immunology, Vol.73, No.5, 470-473, 2012.

(要約): CC chemokine ligand 20 (CCL20) is related to T-helper (Th)-17 cell migration, and Th17 cells play important roles in exacerbation in periodontal disease. However, the effect of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) on CCL20 production is unknown. In this study, we examined the mechanisms of TWEAK in combination with interleukin (IL)-1-induced CCL20 production in human gingival fibroblasts (HGFs). TWEAK alone did not induce CCL20 production in HGFs. However, TWEAK enhanced CCL20 expression from IL-1-stimulated HGFs in a dose-dependent manner. Inhibitors of p38 mitogen-activated protein kinase, extracellular signal-regulated kinase (ERK), protein kinase B (Akt), and nuclear factor B (NF-B) significantly inhibited CCL20 production in TWEAK and IL-1-stimulated HGFs. Western blot analysis revealed that phosphorylations of ERK, Akt, and inhibitor of NF-B were enhanced in TWEAK and IL-1-treated HGFs. These data suggest that TWEAK is positively related to Th17 cell migration in periodontally diseased tissues to enhance CCL20 production in IL-1-stimulated HGFs.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.humimm.2012.02.021
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22425737; ● Search Scopus @ Elsevier (PMID): 22425737; ● Search Scopus @ Elsevier (DOI): 10.1016/j.humimm.2012.02.021

(DOI: 10.1016/j.humimm.2012.02.021, PubMed: 22425737) Tsuyoshi Fujita, Hiromichi Yumoto, Hideki Shiba, Kazuhisa Ouhara, Tsuyoshi Miyagawa, Takayoshi Nagahara, Shinji Matsuda, Hiroyuki Kawaguchi, Takashi Matsuo, Shinya Murakami and Hidemi Kurihara :
Irsogladine maleate regulates epithelial barrier function in TNF--stimulated human gingival epithelial cells,
Journal of Periodontal Research, Vol.47, No.1, 55-61, 2012.

(要約): As epithelial cells function as a mechanical barrier, the permeability of the gingival epithelial cell layer indicates a defensive capability against invasion by periodontal pathogens. We have reported the expression of claudin-1 and E-cadherin, key regulators of permeability, in the gingival junctional epithelium. Irsogladine maleate (IM) is a medication for gastric ulcers and also regulates Aggregatibacter actinomycetemcomitans-stimuated chemokine secretion and E-cadherin expression in gingival epithelium. In this study, we have further investigated the effects of IM on the barrier functions of gingival epithelial cells under inflammatory conditions. We examined the permeability, and the expression of claudin-1 and E-cadherin, in human gingival epithelial cells (HGECs) stimulated with tumor necrosis factor (TNF)-α, with or without IM. TNF-α increased the permeability of HGECs, and IM abolished the increase. TNF-α reduced the expression of E-cadherin in HGECs, and IM reversed the reduction. In addition, immunofluorescence staining showed that TNF-α disrupted claudin-1 expression in HGECs, and IM reversed this effect. The results suggest that IM reverses the TNF-α-induced disruption of the gingival epithelial barrier by regulating E-cadherin and claudin-1.
(キーワード): Adult / Blotting, Western / Cadherins / Cell Culture Techniques / Cell Line / Cell Membrane Permeability / Claudin-1 / Electric Impedance / Epithelial Attachment / Epithelial Cells / Female / Fluorescein / Fluorescent Antibody Technique / Fluorescent Dyes / Gingiva / Humans / Male / Membrane Proteins / Real-Time Polymerase Chain Reaction / Tight Junctions / Triazines / Tumor Necrosis Factor-alpha / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0765.2011.01404.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21895660; ● Search Scopus @ Elsevier (PMID): 21895660; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2011.01404.x

(DOI: 10.1111/j.1600-0765.2011.01404.x, PubMed: 21895660) Shibata Shingo, Toshiyuki Suge, Tomoko Kimura, Ishikawa Kunio and Takashi Matsuo :
Antibacterial activity of ammonium hexafluorosilicate solution with antimicrobial agents for the prevention of dentin caries,
American Journal of Dentistry, Vol.25, No.1, 31-34, 2012.

(要約): This study evaluated the antibacterial activity of the SiF solution with the addition of antibacterial agents on a Streptococcus mutans biofilm. Various antibacterial SiF solutions were prepared by adding chlorhexidine, cetylpyridinium chloride, isopropyl methylphenol, or epigallocatechin gallate. Hydroxyapatite pellets treated with several SiF solutions were immersed in BHI inoculated with S. mutans standardized suspension. The number of S. mutans cells adhered to each pellet was evaluated. SiF with the addition of CPC was the most effective for reducing the adherence of bacteria and inhibiting the formation ofbiofilm, showing the same level as AgF, In contrast, the addition of other antibacterial agents to SiF reduced the original antibacterial activity of SiF solution.
(キーワード): Ammonium hexafluorosilicate / Fluoride / Dentin caries
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22558689; ● Search Scopus @ Elsevier (PMID): 22558689

(PubMed: 22558689) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Interleukin (IL)-17A synergistically enhances CC chemokine ligand 20 production in IL-1-stimulated human gingival fibroblasts.,
Human Immunology, Vol.73, No.1, 26-30, 2012.

(要約): CC chemokine ligand 20 (CCL20) plays a pivotal role in the recruitment of T-helper (Th)-17 cells and thus in the development of periodontal disease, but the effect of simultaneous interleukin (IL)-17A and IL-1 stimulation on CCL20 production in human gingival fibroblasts (HGFs) are not known. In this study, we investigated the mechanisms of IL-1- and IL-17A-induced CCL20 production in HGFs. IL-17A synergistically enhanced CCL20 production from IL-1-stimulated HGFs in a concentration-dependent manner. Extracellular signal-regulated kinase (ERK) and inhibitor of nuclear factor (NF)-B- phosphorylation were increased in IL-1- and IL-17A-stimulated HGFs. Inhibitors of or ERK and NF-B decreased IL-1- and IL-17A-induced CCL20 production. IL-1 stimulation elevated IL-17 receptor C expression on HGFs. These data suggest that IL-1 is actively related to Th17 cell migration into peripheral tissues to induce production of the Th17 chemokine, CCL20. Therefore, IL-1 might be a therapeutic target for Th17-related diseases, such as periodontal disease and arthritis.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.humimm.2011.10.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22019504; ● Search Scopus @ Elsevier (PMID): 22019504; ● Search Scopus @ Elsevier (DOI): 10.1016/j.humimm.2011.10.004

(DOI: 10.1016/j.humimm.2011.10.004, PubMed: 22019504) 坂東直樹, 富永敏彦, 湯本浩通, 住友孝史, 平尾早希, 平尾功治, 松尾敬志 :
電磁波照射の歯内療法への応用ーEMAT (Electro-Magnetic Apical Treatment)ー,
日本歯内療法学会雑誌, Vol.32, No.3, 184-200, 2011年.

(キーワード): electro-magnetic wave / periapical lesion / non-surgical treatment / refractory periapical periodontitis
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680166084096

(CiNii: 1390282680166084096) Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Kanako Takahashi, Hiromichi Yumoto and Takashi Matsuo :
Roles of dental pulp fibroblasts in the recognition of bacterium-related factors and subsequent development of pulpitis,
Japanese Dental Science Review, Vol.47, No.2, 161-166, 2011.

(キーワード): Toll-like receptor (TLR) / Nucleotide-binding oligomerization domain (NOD) / Dental pulp fibroblast / Cytokine / Pulpitis
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jdsr.2011.02.001
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1360565165959499264; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jdsr.2011.02.001

(DOI: 10.1016/j.jdsr.2011.02.001, CiNii: 1360565165959499264) Shinya Horiuchi, Shingo Kuroda, Masahiro Hiasa, Toshiyuki Suge, Seitaro Saku, Kenichi Hamada, Takashi Matsuo, Kenzo Asaoka and Eiji Tanaka :
Reinforcement of bond strength of self-etching orthodontic adhesive.,
The Angle Orthodontist, Vol.82, No.1, 30-35, 2011.

(要約): Abstract Objective: To determine the reinforcement of bond strength of a self-etching system by applying a pretreatment agent. Materials and Methods: Sixty extracted human premolars were used in this study. The enamel surfaces were treated with four pretreatment agents?phosphoric acid, polyacrylic acid, citric acid, and ammonium hexafluorosilicate (SiF)?and were examined under a scanning electron microscope. Afterward, orthodontic brackets were bonded with a self-etching adhesive system (n ?=? 10 for each agent), and shear bond strength was measured through a debonding process. The adhesive remnant index (ARI) was also assessed. Results: Enamel surfaces treated with polyacrylic acid seemed almost the same as intact enamel. Treatment with SiF induced slight shallow depressions compared with the intact enamel. On the other hand, enamel surfaces treated with citric acid and phosphoric acid showed severe etching patterns. All pretreatments increased the bond strength, but SiF-treated specimens revealed the greatest strength (12.201 ? 1.048?MPa), followed by polyacrylic acid (12.030 ? 2.103?MPa). The control group with no pretreatment showed the least strength (9.078 ? 1.678?MPa). All pretreatments increased ARI score compared with the control group. Conclusions: Surface conditioning before bracket adhesion could reinforce the bond strength of the self-etching adhesive system, resulting in a more reliable bonding system.
(出版サイトへのリンク): ● Publication site (DOI): 10.2319/012011-39.1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21751834; ● Search Scopus @ Elsevier (PMID): 21751834; ● Search Scopus @ Elsevier (DOI): 10.2319/012011-39.1

(DOI: 10.2319/012011-39.1, PubMed: 21751834) Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Tadashi Nakanishi, Hideaki Nakae and Takashi Matsuo :
Black tea polyphenol inhibits CXCL10 production in oncostatin M-stimulated human gingival fibroblasts.,
International Immunopharmacology, Vol.11, No.6, 670-674, 2011.

(要約): CXC chemokine ligand 10 (CXCL10) plays an important role in the infiltration of Th1 cells and thus in the exacerbation of periodontal disease. Theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, has some beneficial effects but the effect of TFDG on CXCL10 production from human gingival fibroblasts (HGFs) is uncertain. In this study, we investigated the mechanisms by which TFDG may inhibit oncostatin M (OSM)-induced CXCL10 production in human gingival fibroblasts. TFDG prevented OSM-mediated CXCL10 production by HGFs in a dose dependent manner. TFDG significantly inhibited OSM-induced phosphorylation of c-Jun N terminal kinase (JNK), protein kinase B (Akt) (Ser473) that are related to CXCL10 production from OSM-stimulated HGFs. In addition, TFDG suppressed OSM receptor (OSMR) β expression on HGFs. These data provide a novel mechanism where the black tea flavonoid, theaflavin, could provide direct benefits in periodontal disease.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.intimp.2011.01.009
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21255696; ● Search Scopus @ Elsevier (PMID): 21255696; ● Search Scopus @ Elsevier (DOI): 10.1016/j.intimp.2011.01.009

(DOI: 10.1016/j.intimp.2011.01.009, PubMed: 21255696) Katsuhiko Hirota, Hiromichi Yumoto, K Miyamoto, N Yamamoto, Keiji Murakami, Yumi Hoshimo, Takashi Matsuo and Yoichiro Miyake :
MPC-Polymer Reduces Adherence and Biofilm Formation by Oral Bacteria.,
Journal of Dental Research, Vol.90, No.7, 900-905, 2011.

(要約): Oral biofilms such as dental plaque cause dental caries and periodontitis, as well as aspiration pneumonia and infectious endocarditis by translocation. Hence, the suppression of oral biofilm formation is an issue of considerable importance. Mechanical removal, disinfectants, inhibition of polysaccharide formation, and artificial sugar have been used for the reduction of oral biofilm. From the viewpoint of the inhibition of bacterial adherence, we investigated whether aqueous biocompatible 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer can reduce streptococcal colonization and biofilm formation. We examined the effects of MPC-polymer on streptococcal adherence to saliva-coated hydroxyapatite and oral epithelial cells, and the adherence of Fusobacterium nucleatum to streptococcal biofilm. MPC-polymer application markedly inhibited both the adherence and biofilm formation of Streptococcus mutans on saliva-coated hydroxyapatite and streptococcal adherence to oral epithelial cells, and reduced the adherence of F. nucleatum to streptococcal biofilms. A small-scale clinical trial revealed that mouthrinsing with MPC-polymer inhibited the increase of oral bacterial numbers, especially of S. mutans. These findings suggest that MPC-polymer is a potent inhibitor of bacterial adherence and biofilm development, and may be useful to prevent dental-plaque-related diseases. (UMIN Clinical Trial Registry UMIN000003471). Abbreviations: QOL, quality of life; MPC, 2-methacryloyloxyethyl phosphorylcholine; BHI, brain-heart infusion broth; PBS, phosphate-buffered saline; SEM, scanning electron microscopy; CFU, colony-forming unit.
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/0022034511402996
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21447697; ● Summary page in Scopus @ Elsevier: 2-s2.0-84884211413

(DOI: 10.1177/0022034511402996, PubMed: 21447697, Elsevier: Scopus) J Li, Katsuhiko Hirota, Hiromichi Yumoto, Takashi Matsuo, Yoichiro Miyake and Tetsuo Ichikawa :
Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms.,
Journal of Applied Microbiology, Vol.109, No.6, 2183-2190, 2010.

(要約): The germicidal effects of UVA-LED irradiation (365 nm, 0·28 mW cm(-2) , in pulsed or continuous mode) on Candida albicans or Escherichia coli biofilms were evaluated by determining colony-forming units. The morphological change of microbial cells in biofilms was observed using scanning electron microscopy. After 5-min irradiation, over 90% of viable micro-organisms in biofilms had been killed, and pulsed irradiation (1-1000 Hz) had significantly greater germicidal ability than continuous irradiation. Pulsed irradiation (100 Hz, 60 min) almost completely killed micro-organisms in biofilm (>99·9%), and 20-min irradiation greatly damaged both microbial species. Interestingly, few hyphae were found in irradiated Candida biofilms. Moreover, mannitol treatment, a scavenger of hydroxyl radicals (OH(•) ), significantly protected viable micro-organisms in biofilms from UVA-LED irradiation.
(キーワード): Biofilms / Candida albicans / Colony Count, Microbial / Disinfection / 大腸菌 (Escherichia coli) / Free Radical Scavengers / Hydroxyl Radical / Mannitol / Microbial Viability / Ultraviolet Rays
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2672.2010.04850.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20854456; ● Summary page in Scopus @ Elsevier: 2-s2.0-78449262889

(DOI: 10.1111/j.1365-2672.2010.04850.x, PubMed: 20854456, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Oncostatin M synergistically induces CXCL10 and ICAM-1 expression in IL-1beta-stimulated-human gingival fibroblasts.,
Journal of Cellular Biochemistry, Vol.111, No.1, 40-48, 2010.

(要約): Periodontitis is a chronic bacterial infection of tooth-supporting structures. T-helper type 1 (Th1) cells are related to the exacerbation of periodontal disease. Human gingival fibroblasts (HGFs), the major cell type in periodontal connective tissues, are involved in immunological response in periodontal tissues. However, it is uncertain whether HGFs are related to Th1 response. Chemokine (C-X-C motif) ligand 10 (CXCL10) is a cytokine, that is related to Th1 cells migration. Intercellular adhesion molecule (ICAM)-1 is involved in Th1 cells retention and activation in inflamed tissue. The aim of this study is to examine the effect of oncostatin M (OSM) on CXCL10 and ICAM-1 expression in HGFs. OSM stimulation induced CXCL10 and ICAM-1 expression in HGFs. Moreover, the synergistic effects of CXCL10 release and ICAM-1 expression in HGFs were observed with combined stimulation of interleukin (IL)-1beta and OSM. OSM increased type 1 IL-1 receptor (IL-1R1) expression, and IL-1beta enhanced OSMRbeta expression on HGFs. IL-1beta + OSM stimulation enhanced the phosphorylation of inhibitor of nuclear factor kappaB (IkappaB)-alpha, signal transducer and activator of transcription (STAT)3, c-Jun N terminal kinase (JNK), and protein kinase B (Akt) compared to OSM or IL-1beta stimulation. CXCL10 production from OSM + IL-1beta stimulated HGFs was suppressed by nuclear factor (NF)-kappaB, STAT3, JNK, and phosphoinositide-3-kinase (PI3K) inhibitors. On the other hand, only NF-kappaB and STAT3 inhibitors suppressed ICAM-1 expression enhanced by OSM + IL-1beta treatment. These effects of OSM and IL-1beta may promote Th1 cells infiltration and retention in periodontally diseased tissues and be related to exacerbation of periodontal disease. J. Cell. Biochem. 111: 40-48, 2010. (c) 2010 Wiley-Liss, Inc.
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jcb.22648
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20680966; ● Search Scopus @ Elsevier (PMID): 20680966; ● Search Scopus @ Elsevier (DOI): 10.1002/jcb.22648

(DOI: 10.1002/jcb.22648, PubMed: 20680966) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Tadashi Nakanishi, Hideaki Nakae and Takashi Matsuo :
Tea polyphenols inhibit IL-6 production in tumor necrosis factor superfamily 14-stimulated human gingival fibroblasts.,
Molecular Nutrition & Food Research, Vol.54 Suppl 2, S151-8, 2010.

(要約): IL-6 is well recognized to be a potent bone resorptive agent and thus in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins in green tea, and theaflavin-3,3'-digallate (TFDG), polyphenol in black tea, have multiple beneficial effects, but the effects of catechins and theaflavins on IL-6 production in human gingival fibroblasts (HGFs) are not known. In this study, we investigated the mechanisms by which EGCG, ECG, and TFDG inhibit tumor necrosis factor superfamily 14 (TNFSF14)-induced IL-6 production in HGFs. We detected TNFSF14 mRNA expression in human diseased periodontal tissues. TNFSF14 increased IL-6 production in HGFs in a concentration-dependent manner. EGCG, ECG, and TFDG prevented TNFSF14-mediated IL-6 production in HGFs. EGCG, ECG, and TFDG prevented TNFSF14-induced extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and nuclear factor-kappaB activation in HGFs. Inhibitors of ERK, JNK, and nuclear factor-kappaB decreased TNFSF14-induced IL-6 production. In addition, EGCG, ECG, and TFDG attenuated TNFSF14 receptor expression on HGFs. These data provide a novel mechanism through which the green tea and black tea polyphenols could be used to provide direct benefits in periodontal disease.
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/mnfr.200900549
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20461739; ● Search Scopus @ Elsevier (PMID): 20461739; ● Search Scopus @ Elsevier (DOI): 10.1002/mnfr.200900549

(DOI: 10.1002/mnfr.200900549, PubMed: 20461739) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Tadashi Nakanishi, Hideaki Nakae and Takashi Matsuo :
Catechins inhibit CXCL10 production from oncostatin M-stimulated human gingival fibroblasts,
The Journal of Nutritional Biochemistry, Vol.21, No.7, 659-664, 2010.

(要約): CXC chemokine ligand 10 (CXCL10) plays a pivotal role in the recruitment of Th1 cells and, thus, in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins derived from green tea, have multiple beneficial effects, but the effects of catechins on CXCL10 production from human gingival fibroblasts (HGFs) is not known. In this study, we investigated the mechanisms by which EGCG and ECG inhibit oncostatin M (OSM)-induced CXCL10 production in HGFs. HGFs constitutively expressed glycoprotein 130 and OSM receptor beta (OSMR beta), which are OSM receptors. OSM increased CXCL10 production in a concentration-dependent manner. EGCG and ECG prevented OSM-mediated CXCL10 production by HGFs. Inhibitors of p38 mitogen-activated protein kinase, c-Jun N-terminal kinase (JNK), phosphatidylinositol-3-OH kinase and signal transducer and activator of transcription (STAT)3 decreased OSM-induced CXCL10 production. EGCG significantly prevented OSM-induced phosphorylation of JNK, Akt (Ser473) and STAT3 (Tyr705 and Ser727). ECG prevented phosphorylation of JNK and Akt (Ser473). In addition, EGCG and ECG attenuated OSMR beta expression on HGFs. These data provide a novel mechanism through which the green tea flavonoids, catechins, can provide direct benefits in periodontal disease.
(キーワード): Anti-Inflammatory Agents, Non-Steroidal / Antioxidants / Catechin / Cells, Cultured / Chemokine CXCL10 / Cytokine Receptor gp130 / Enzyme Inhibitors / Fibroblasts / Gene Expression Regulation / Gingiva / Humans / Oncostatin M / Oncostatin M Receptor beta Subunit / Osmolar Concentration / Periodontal Diseases / Phosphorylation / Signal Transduction
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jnutbio.2009.04.005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19616927; ● Search Scopus @ Elsevier (PMID): 19616927; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jnutbio.2009.04.005

(DOI: 10.1016/j.jnutbio.2009.04.005, PubMed: 19616927) Kouji Hirao, Hiromichi Yumoto, Tadashi Nakanishi, Kayo Mukai, Kanako Takahashi, Daisuke Takegawa and Takashi Matsuo :
Tea catechins reduce inflammatory reactions via mitogen-activated protein kinase pathways in toll-like receptor 2 ligand-stimulated dental pulp cells.,
Life Sciences, Vol.86, No.17-18, 654-660, 2010.

(要約): These findings suggest that catechins might be useful therapeutically as an anti-inflammatory modulator of dental pulpal inflammation.
(キーワード): 抗炎症剤 / カテキン (catechin) / Cells, Cultured / 歯髄細胞 / Enzyme-Linked Immunosorbent Assay / Fibroblasts / Humans / Inflammation / Inflammation Mediators / Ligands / Mitogen-Activated Protein Kinases / Phosphorylation / Receptors, Pattern Recognition / Reverse Transcriptase Polymerase Chain Reaction / Signal Transduction / Streptococcus / Tea / Toll-Like Receptor 2 / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2010.02.017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20176036; ● Search Scopus @ Elsevier (PMID): 20176036; ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2010.02.017

(DOI: 10.1016/j.lfs.2010.02.017, PubMed: 20176036) Tadashi Nakanishi, Kayo Mukai, Hiromichi Yumoto, Kouji Hirao, Yoshitaka Hosokawa and Takashi Matsuo :
Anti-inflammatory effect of catechin on cultured dental pulp cells affected by bacteria-derived factors,
European Journal of Oral Sciences, Vol.118, No.2, 145-150, 2010.

(要約): Catechins (bioactive polyphenols in green tea) are known to exhibit potent anti-inflammatory properties. However, the anti-inflammatory effects of catechins on inflamed dental pulp tissue are not known. In this study, we investigated the effect of epigallocatechin-3-gallate (EGCG) and epicatechin gallate (ECG), the major components of green tea catechins, on the expression of pro-inflammatory cytokines and adhesion molecules in human dental pulp cells stimulated with bacteria-derived factors such as lipopolysaccharide (LPS) and peptidoglycan (PG). The expression of interleukin (IL)-6 and of IL-8 was examined using the reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assays. The expression of intercellular adhesion molecule-1 (ICAM-1) and of vascular cell adhesion molecule-1 (VCAM-1) on dental pulp cells was analyzed using flow cytometry. The presence of EGCG and ECG significantly reduced, in a concentration-dependent manner, the expression of IL-6 and IL-8 in dental pulp cells exposed to LPS or PG. Increased expression of ICAM-1 and VCAM-1 on the dental pulp cells in response to bacterial components was also decreased by treatment with EGCG and ECG. These findings suggest that green tea catechins may prevent the exacerbation of pulpitis.
(キーワード): Anti-Inflammatory Agents / Antioxidants / Catechin / Cells, Cultured / Dental Pulp / Escherichia coli / Flow Cytometry / Humans / Intercellular Adhesion Molecule-1 / Interleukin-6 / Interleukin-8 / Lipopolysaccharides / Peptidoglycan / Reverse Transcriptase Polymerase Chain Reaction / Staphylococcus aureus / Time Factors / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0722.2010.00714.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20487003; ● Search Scopus @ Elsevier (PMID): 20487003; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0722.2010.00714.x

(DOI: 10.1111/j.1600-0722.2010.00714.x, PubMed: 20487003) Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Proinflammatroy effects of muramyldipeptide on human gingival fibroblasts,
Journal of Periodontal Research, Vol.45, No.2, 193-199, 2010.

(要約): Because human gingival fibroblasts (HGFs) are the predominant cells in periodontal tissues, we hypothesized that HGFs are contributed to receptors for components of bacteria. In this study, we focused on expression and function of nucleotide binding oligomerization domain 2 (NOD2) in HGFs, which is a mammalian cytosolic pathogen recognition molecule. Expression of NOD2 in HGFs was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry. Production of interleukin (IL)-6, IL-8, cc chemokine ligand2, cxc chemokine ligand10 (CXCL10) and CXCL11 from HGFs was examined by enzyme-linked immunosorbent assay (ELISA). We used RT-PCR and immunohistochemistry to detect the NOD2 expression in human gingival tissues. We found clear NOD2 expression in HGFs. Upon stimulation with NOD2 agonist, muramyldipeptide (MDP), production of proinflammatory cytokines was enhanced. Moreover, MDP-induced production of proinflammatory cytokines was inhibited in a different manner by mitogen-activated protein kinase inhibitors and phosphatidylinositol 3-kinase inhibitor. Furthermore, MDP enhanced CXCL10 and CXCL11 productions by tumor necrosis factor-alpha (TNF-alpha)- or interferon-gamma (IFN-gamma)-stimulated HGFs, although MDP alone did not induce these chemokines. TNF-alpha and IFN-gamma increased NOD2 expression in HGFs. In addition, we detected NOD2 expression in mononuclear cells and HGFs in periodontally diseased tissues. These findings indicate that MDP which induces production of cytokines and chemokines from HGFs is related to the pathogenesis of periodontal disease.
(キーワード): Acetylmuramyl-Alanyl-Isoglutamine / Adult / Anthracenes / Calcium-Calmodulin-Dependent Protein Kinases / Cells, Cultured / Chemokine CCL2 / Chemokine CXCL10 / Chemokine CXCL11 / Chromones / Chronic Periodontitis / Enzyme Inhibitors / Female / Fibroblasts / Flavonoids / Gingiva / Humans / Imidazoles / Inflammation Mediators / Interferon-gamma / Interleukin-6 / Interleukin-8 / JNK Mitogen-Activated Protein Kinases / Male / Middle Aged / Morpholines / Nod2 Signaling Adaptor Protein / Periodontal Attachment Loss / Periodontal Pocket / Phosphatidylinositol 3-Kinases / Pyridines / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0765.2009.01217.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20470259; ● Search Scopus @ Elsevier (PMID): 20470259; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2009.01217.x

(DOI: 10.1111/j.1600-0765.2009.01217.x, PubMed: 20470259) Toshiyuki Suge, Akiko Kawasaki, Ishikawa Kunio, Takashi Matsuo and Ebisu Shigeyuki :
Effects of ammonium hexafluorosilicate concentration on dentin tubule occlusion and composition of the precipitate,
Dental Materials, Vol.26, No.1, 29-34, 2010.

(要約): Ammonium hexafluorosilicate [SiF: (NH(4))(2)SiF(6)] was prepared in order to overcome the tooth discoloration caused by diamine silver fluoride [AgF: (NH(3))(2)AgF] application. We employed a single concentration of SiF solution in our previous study; therefore, it is still unclear how the concentration of SiF solution affects the occlusion of dentin tubules and composition of the precipitate. The aim of this study was to evaluate the effects of changing the concentration of SiF on its clinical use as a dentin hypersensitivity treatment. To simulate dentin tubules subject to dentin hypersensitivity, dentin disks were treated with EDTA for 2 min. Then, the disks were treated with several concentrations of SiF solution (from 100 to 19,400 ppm) for 3 min. The occlusion of dentin tubules was evaluated using scanning electron microscopy (SEM), and the composition of the precipitate formed in the tubules after SiF treatment was assessed using energy dispersive X-ray analysis (EDXA). SEM photographs demonstrated that dentin tubules after treatment with SiF were occluded homogeneously and fully regardless of the concentration of SiF solution. The Ca/P molar ratio of the precipitate formed in dentin tubules after SiF treatment was increased with the concentration of SiF solution. It was concluded that the capacity to occlude dentin tubules was the same regardless of the concentration of SiF solution. However, the composition of the precipitate formed in the tubules was dependent on the concentration of SiF solution.
(キーワード): Calcium / Chemical Precipitation / Dentin / Dentin Desensitizing Agents / Dentin Permeability / Dentin Sensitivity / Electron Probe Microanalysis / Fluorides / Humans / Hydrogen-Ion Concentration / Materials Testing / Microscopy, Electron, Scanning / Phosphorus / Quaternary Ammonium Compounds / Silicic Acid / Silicon Compounds / Time Factors / Tooth Discoloration
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.dental.2009.08.011
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19748664; ● Search Scopus @ Elsevier (PMID): 19748664; ● Search Scopus @ Elsevier (DOI): 10.1016/j.dental.2009.08.011

(DOI: 10.1016/j.dental.2009.08.011, PubMed: 19748664) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
TNFSF14 coordinately enhances CXCL10 and CXCL11 production from IFN-gamma-stimulated human gingival fibroblasts,
Molecular Immunology, Vol.47, No.4, 666-670, 2010.

(要約): TNFSF14 is involved in the pathogenesis of some inflammatory diseases such as arthritis. CXCL10 and CXCL11 recruit Th1 cells, and the productions of these chemokines are related to the exacerbation of some inflammatory diseases including arthritis and periodontal disease. We examined in vitro effects of TNFSF14 on IFN-gamma-induced CXCL10 and CXCL11 production in human gingival fibroblasts (HGFs). HGFs constitutively expressed TNFSF14 receptors, LTbetaR and HVEM. TNFSF14 enhanced IFN-gamma-induced secretion of CXCL10 and CXCL11 from HGFs. IFN-gamma treatment increased HVEM expression on HGFs. TNFSF14 in combination with IFN-gamma resulted in increased activation of p38 MAPK, ERK and IkappaB-alpha compared with TNFSF14 or IFN-gamma alone. Moreover, inhibitors of p38 MAPK, ERK and NF-kappaB abolished the CXCL10 and CXCL11 productions from TNFSF14 with IFN-gamma-stimulated HGFs. These effects of TNFSF14 may promote the infiltration of Th1 cells into lesions with inflammatory diseases. TNFSF14 might act as a proinflammatory cytokine in some inflammatory diseases thus is a candidate therapeutic target.
(キーワード): Chemokine CXCL10 / Chemokine CXCL11 / Extracellular Signal-Regulated MAP Kinases / Fibroblasts / Gingiva / Humans / I-kappa B Proteins / Interferon-gamma / Lymphotoxin beta Receptor / MAP Kinase Kinase Kinases / Phosphorylation / Protein Kinase Inhibitors / Proto-Oncogene Proteins / RNA, Messenger / Receptors, Tumor Necrosis Factor, Member 14 / Signal Transduction / Tumor Necrosis Factor Ligand Superfamily Member 14 / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.molimm.2009.10.018
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19939453; ● Search Scopus @ Elsevier (PMID): 19939453; ● Search Scopus @ Elsevier (DOI): 10.1016/j.molimm.2009.10.018

(DOI: 10.1016/j.molimm.2009.10.018, PubMed: 19939453) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Tadashi Nakanishi, Hideaki Nakae and Takashi Matsuo :
Catechins Inhibit CCL20 Production in IL-17A-stimulated Human Gingival Fibroblasts,
Cellular Physiology and Biochemistry, Vol.24, No.5-6, 391-396, 2009.

(要約): CC chemokine ligand 20 (CCL20) plays a pivotal role in the recruitment of Th17 cells and thus in the development of periodontal disease. Epigallocatechin gallate (EGCG) and epicatechin gallate (ECG), the major catechins in green tea, have multiple beneficial effects, but the effects of catechins on CCL20 production in human gingival fibroblasts (HGFs) are not known. In this study, we investigated the mechanisms by which EGCG and ECG inhibit interleukin (IL)-17A-induced CCL20 production in human gingival fibroblasts. IL-17A increased CCL20 production in HGFs in a concentration-dependent manner. EGCG and ECG prevented IL-17A-mediated CCL20 production in HGFs. Inhibitors of p38 mitogen-activated protein kinase (MAPK) or extracellular signal-regulated kinase (ERK) decreased IL-17A-induced CCL20 production. EGCG and ECG prevented IL-17A-induced phosphorylation of p38 MAPK and ERK in HGFs. In addition, EGCG and ECG attenuated IL-17 receptor expression on HGFs. These data provide a novel mechanism through which the green tea flavonoids catechins could be used to provide direct benefits in periodontal disease.
(キーワード): Antioxidants / Catechin / Cells, Cultured / Chemokine CCL20 / Extracellular Signal-Regulated MAP Kinases / Fibroblasts / Gingiva / Humans / Interleukin-17 / Phosphorylation / p38 Mitogen-Activated Protein Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1159/000257431
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19910679; ● Search Scopus @ Elsevier (PMID): 19910679; ● Search Scopus @ Elsevier (DOI): 10.1159/000257431

(DOI: 10.1159/000257431, PubMed: 19910679) Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi, Kayo Mukai, Tadashi Nakanishi and Takashi Matsuo :
Roles of TLR2, TLR4, NOD2, and NOD1 in pulp fibroblasts.,
Journal of Dental Research, Vol.88, No.8, 762-767, 2009.

(要約): Pulp fibroblasts express various pro-inflammatory mediators leading to marked infiltration of inflammatory cells in the progression of pulpitis. We hypothesized that pulp fibroblasts play roles in the recognition of invaded caries-related bacteria and the subsequent innate immune responses. We found clear expressions of TLR2, NOD1, and NOD2 and a faint expression of TLR4 in human dental pulp fibroblasts (HDPF) by RT-PCR and flow cytometry. We also observed that various pro-inflammatory mediators, including cytokines, chemokines, adhesion molecules, prostaglandin E(2) and its key enzyme COX-2, not iNOS or caspase-1, were markedly up-regulated by stimulation with these TLR and NOD agonists. More over, the NOD2 agonist acted synergistically with the TLR2, not the TLR4, agonist to stimulate the production of pro-inflammatory mediators in HDPF. These findings indicate that TLR2, TLR4, NOD2, and NOD1 in HDPF are functional receptors, and NOD2 is a modulator of signals transmitted through TLR2 in pulpal immune responses, leading to progressive pulpitis.
(キーワード): Acetylmuramyl-Alanyl-Isoglutamine / Apoptosis / Cells, Cultured / Chemokine CCL2 / Chemokine CXCL10 / Cyclooxygenase 2 / Dental Pulp / Diaminopimelic Acid / Dinoprostone / Escherichia coli / Fibroblasts / Humans / Inflammation Mediators / Interleukin-6 / Interleukin-8 / Lipopeptides / Lipopolysaccharides / Nod1 Signaling Adaptor Protein / Nod2 Signaling Adaptor Protein / Pulpitis / Signal Transduction / Streptococcus mutans / Toll-Like Receptor 2 / Toll-Like Receptor 4 / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/0022034509341779
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19734466; ● Search Scopus @ Elsevier (PMID): 19734466; ● Search Scopus @ Elsevier (DOI): 10.1177/0022034509341779

(DOI: 10.1177/0022034509341779, PubMed: 19734466) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Human gingival fibroblasts express functional chemokine receptor CXCR6.,
Clinical and Experimental Immunology, Vol.156, No.3, 413-418, 2009.

(要約): We have reported that CXCL16, a recently discovered transmembrane chemokine, is expressed in human gingival fibroblasts (HGF). However, it is not known whether HGF express CXCR6, the receptor for CXCL16, or CXCL16 affects HGF biology. We have shown that HGF expressed CXCR6 by reverse transcription-polymerase chain reaction and flow cytometric analysis. Moreover, we elucidated that tumour necrosis factor (TNF)-alpha and cytosine-guanine dinucleotide (CpG) DNA (Toll-like receptor-9 ligand) treatment enhanced CXCR6 expression by HGF. Interleukin (IL)-4, IL-13 and CpG DNA up-regulated CXCR6 expression by TNF-alpha-stimulated HGF. On the other hand, IL-1beta and interferon-gamma inhibited CXCR6 expression on TNF-alpha-treated HGF. CXCL16 treatment induced HGF proliferation and phosphorylation of extracellular regulated kinase (ERK) and protein kinase B (AKT) in HGF. In conclusion, HGF expressed CXCR6 functionally, because CXCL16 induced HGF proliferation and ERK and AKT phosphorylation in HGF. These results indicate that CXCL16 may play an important role in the pathogenesis and remodelling in periodontally diseased tissues.
(キーワード): Cell Proliferation / Cells, Cultured / Chemokines, CXC / CpG Islands / Cytokines / Extracellular Signal-Regulated MAP Kinases / Fibroblasts / Gingiva / Humans / Ligands / Proto-Oncogene Proteins c-akt / Receptors, Chemokine / Receptors, Scavenger / Receptors, Virus / Reverse Transcriptase Polymerase Chain Reaction / Toll-Like Receptors
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2009.03915.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19438592; ● Search Scopus @ Elsevier (PMID): 19438592; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2009.03915.x

(DOI: 10.1111/j.1365-2249.2009.03915.x, PubMed: 19438592) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Cytokines differentially regulate CXCL10 production by interferon-gamma-stimulated or tumor necrosis factor-alpha-stimulated human gingival fibroblasts.,
Journal of Periodontal Research, Vol.44, No.2, 225-231, 2009.

(要約): CXC chemokine 10 (CXCL10) activates CXC chemokine receptor 3 (CXCR3) and attracts activated T-helper 1 cells. In this study we examined the effects of cytokines on CXCL10 production by human gingival fibroblasts. Human gingival fibroblasts were exposed to pro-inflammatory cytokines (interleukin-1beta, tumor necrosis factor-alpha), a T-helper 1 cytokine (interferon-gamma), T-helper 2 cytokines (interleukin-4, interleukin-13), T-helper 17 cytokines (interleukin-17A, interleukin-22) and regulatory T-cell cytokines (interleukin-10, transforming growth factor-beta1) for 24 h. CXCL10 production by human gingival fibroblasts was examined by enzyme-linked immunosorbent assay. Human gingival fibroblasts produced CXCL10 protein upon stimulation with interleukin-1beta, tumor necrosis factor-alpha and interferon-gamma. Treatment of human gingival fibroblasts with interferon-gamma in combination with tumor necrosis factor-alpha or interleukin-1beta resulted in a synergistic production of CXCL10. However, interleukin-4 and interleukin-13 inhibited CXCL10 production by interferon-gamma-stimulated or tumor necrosis factor-alpha-stimulated-human gingival fibroblasts. On the other hand, interleukin-17A and interleukin-22 enhanced CXCL10 production by human gingival fibroblasts treated with interferon-gamma and inhibited CXCL10 production by tumor necrosis factor-alpha-stimulated human gingival fibroblasts. Furthermore, the anti-inflammatory cytokine, interleukin-10, inhibited CXCL10 production by both interferon-gamma- and tumor necrosis factor-alpha-stimulated human gingival fibroblasts, but transforming growth factor-beta1 enhanced interferon-gamma-mediated CXCL10 production by human gingival fibroblasts. These results mean that the balance of cytokines in periodontally diseased tissue may be essential for the control of CXCL10 production by human gingival fibroblasts, and the production of CXCL10 might be important for the regulation of T-helper 1 cell infiltration in periodontally diseased tissue.
(キーワード): Adult / Cells, Cultured / Chemokine CXCL10 / Cytokines / Female / Fibroblasts / Gingiva / Humans / Inflammation Mediators / Interferon-gamma / Interleukins / Periodontitis / Th1 Cells / Transforming Growth Factor beta1 / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0765.2008.01124.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18973545; ● Search Scopus @ Elsevier (PMID): 18973545; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2008.01124.x

(DOI: 10.1111/j.1600-0765.2008.01124.x, PubMed: 18973545) Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Taniguchi Tomonori, Yamamoto Akitake, Tsuneko Ono, Takashi Matsuo and Yoichiro Miyake :
Heterologous expression of a histone-like protein from Streptococcus intermedius in Escherichia coli alters the nucleoid structure and inhibits the growth of E. coli,
FEMS Microbiology Letters, Vol.288, No.1, 68-75, 2008.

(要約): Escherichia coli failed to survive after transformation with a Streptococcus intermedius histone-like protein gene (Si-hlp) and its promoter-harbored plasmid. The promoter function of Si-hlp in E. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. The inhibitory effect of Si-HLP on E. coli viability was verified by a tetracycline-inducible gene expression system. Further study suggested that Si-HLP may alter the bacterial nucleoid structure, leading to the growth inhibition of E. coli.
(キーワード): Bacterial Proteins / Cell Nucleolus / Cloning, Molecular / DNA, Bacterial / DNA, Superhelical / 大腸菌 (Escherichia coli) / 遺伝子発現 (gene expression) / Histones / Streptococcus intermedius
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1574-6968.2008.01327.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18761672; ● Summary page in Scopus @ Elsevier: 2-s2.0-52649125775

(DOI: 10.1111/j.1574-6968.2008.01327.x, PubMed: 18761672, Elsevier: Scopus) Kanako Takahashi, Tadashi Nakanishi, Hiromichi Yumoto, Tomohiko Adachi and Takashi Matsuo :
CCL20 production is induced in human dental pulp upon stimulation by Streptococcus mutans and pro-inflammatory cytokines,
Oral Microbiology and Immunology, Vol.23, No.4, 320-327, 2008.

(要約): Pulpitis is characterized by the marked infiltration of inflammatory cells in response to an invasion of caries-related bacteria. It is well known that chemokines regulate the trafficking of lymphocytes, and CC chemokine ligand 20 (CCL20) has been recently shown to play a crucial role in the recruitment of memory T cells and immature dendritic cells into inflammatory lesions. We previously reported that CCL20 was mainly expressed in microvascular endothelial cells and macrophages that accumulated in inflamed pulp tissues and that its specific receptor, CCR6, was expressed on infiltrated lymphocytes. However, the mechanism of CCL20 expression remains unclear. In this study, we investigated the expression of CCL20 in monocytes/macrophages, endothelial cells, and pulpal fibroblasts after stimulation with Streptococcus mutans, a representative of caries-related bacteria, or proinflammatory cytokines. CCL20 messenger RNA was detected by reverse transcription-polymerase chain reaction in inflamed pulp, but not in clinically normal pulp. By enzyme-linked immunosorbent assay, S. mutans induced a human monocytic cell line, differentiated macrophage-like THP-1 cells, and human umbilical vein endothelial cells (HUVEC) to produce an increased amount of CCL20. Lipoteichoic acid from S. mutans also elicited CCL20 production by HUVEC. Moreover, CCL20 production from pulpal fibroblasts was increased by stimulation with inetrleukin-1beta and tumor necrosis factor-alpha. Our results indicate that CCL20 expression is induced by stimulation with caries-related bacteria that have invaded deeply into the dentinal tubules as well as by proinflammatory cytokines in the inflamed pulpal lesions. It may be involved in the progression of pulpitis via accumulation of inflammatory cells.
(キーワード): Adult / Aged / Cell Differentiation / Cell Line / Cells, Cultured / Chemokine CCL20 / Cytokines / Dental Pulp / Endothelial Cells / Endothelium, Vascular / Female / Fibroblasts / Humans / Inflammation Mediators / Intercellular Adhesion Molecule-1 / Interleukin-1beta / Interleukin-8 / Lipopolysaccharides / Macrophages / Male / Middle Aged / Monocytes / Pulpitis / Streptococcus mutans / Teichoic Acids / Tumor Necrosis Factor-alpha / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1399-302X.2008.00431.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18582332; ● Search Scopus @ Elsevier (PMID): 18582332; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1399-302X.2008.00431.x

(DOI: 10.1111/j.1399-302X.2008.00431.x, PubMed: 18582332) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
CC Chemokine ligand 17 in periodontal diseases: expression in diseased tissues and production by human gingival fibroblasts,
Journal of Periodontal Research, Vol.43, No.4, 471-477, 2008.

(要約): It has been reported that T helper 2 (Th2) cells are related to exacerbation of periodontal disease. However, it is uncertain how the migration of Th2 cells is controlled. In this study, we examined the expression of CC chemokine ligand 17 (CCL17), which is a Th2 chemokine, in periodontal tissues. Moreover, we investigated the effects of cytokines and toll-like receptor (TLR) ligands on the production of CCL17 by human gingival fibroblasts (HGFs). We used immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) to detect CCL17 in periodontal tissues. HGFs were exposed to cytokines and TLR ligands. Expression of CCL17 was examined by RT-PCR and enzyme-linked immunosorbent assay. We used signal transduction inhibitors in some experiments. Both CCL17 and its receptor, CC chemokine receptor 4 (CCR4), were expressed in diseased periodontal tissues. A combination of tumour necrosis factor alpha (TNF-alpha) and interleukin (IL)-4/IL-13 increased CCL17 expression. Moreover, treatment of HGFs with a low dose of interferon-gamma (IFN-gamma) in combination with TNF-alpha and IL-4 or IL-13 had synergistic effects on the production of CCL17, whereas a high dose of IFN-gamma inhibited CCL17 production. Furthermore, Escherichia coli (E. coli) lipopolysaccharide (TLR4 ligand) and Pam3CSK4 (TLR2 ligand) inhibited CCL17 production by TNF-alpha + IL-4-stimulated HGFs, while CpG DNA (TLR9 ligand) enhanced TNF-alpha + IL-4 induced-CCL17 production by HGFs. Furthermore, a c-Jun NH2 terminal kinase (JNK) inhibitor, a phosphatidylinositol-3-kinase (PI3K) inhibitor and a nuclear factor kappa B (NF-kappa B) inhibitor inhibited CCL17 production by HGFs. These results suggest that the CCL17 produced by HGFs may be involved in the migration of Th2 cells into inflamed tissues, and provide evidence that CCL17 production is controlled by cytokines and TLR ligands in periodontal disease.
(キーワード): Chemokine CCL17 / Cytokines / Escherichia coli / Fibroblasts / Gingiva / Humans / Interferon-gamma / Interleukin-13 / Interleukin-4 / JNK Mitogen-Activated Protein Kinases / Ligands / Lipopeptides / Lipopolysaccharides / NF-kappa B / Peptides / Periodontal Diseases / Phosphatidylinositol 3-Kinases / Receptors, CCR4 / Reverse Transcriptase Polymerase Chain Reaction / Th2 Cells / Toll-Like Receptor 2 / Toll-Like Receptor 4 / Toll-Like Receptor 9 / Toll-Like Receptors / Tumor Necrosis Factor-alpha
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0765.2007.01080.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18557811; ● Search Scopus @ Elsevier (PMID): 18557811; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0765.2007.01080.x

(DOI: 10.1111/j.1600-0765.2007.01080.x, PubMed: 18557811) Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Adrenomedullin suppresses tumour necrosis factor alpha-induced CXC chemokine ligand 10 production by human gingival fibroblasts.,
Clinical and Experimental Immunology, Vol.152, No.3, 568-575, 2008.

(要約): Periodontal disease is an inflammatory disorder characterized by the involvement of chemokines that are important for the recruitment of leucocytes. Several cytokines, including tumour necrosis factor alpha (TNF-alpha), are involved in regulating levels of chemokines in periodontal disease. CXC chemokine ligand 10 (CXCL10) is a chemokine related to the migration of T helper 1 cells. In this study, we examined CXCL10 expression in human gingival fibroblasts (HGFs). Moreover, we investigated the effects of adrenomedullin (AM), which is a multi-functional regulatory peptide, on the production of CXCL10 by HGFs. We revealed that TNF-alpha stimulation induced CXCL10 production by HGFs. HGFs expressed AM and AM receptors, calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein (RAMP) 2, mRNAs constitutively. AM treatment supressed CXCL10 production by TNF-alpha-stimulated HGFs. Moreover, we elucidated that AM produced by HGFs inhibited CXCL10 production by HGFs, because AM antagonist enhanced CXCL10 production by HGFs. TNF-alpha treatment enhanced CRLR and RAMP2 mRNA expression in HGFs. Furthermore, AM is expressed in human periodontal tissues, including both inflamed and clinically healthy tissues. These results suggest that the CXCL10 produced by HGFs may be involved in the migration of leucocytes into inflamed tissues and related to exacerbation of periodontal disease. AM might be a therapeutic target of periodontal disease, because AM can inhibit CXCL10 production by HGFs.
(キーワード): 歯周炎 (periodontal disease)
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2008.03647.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18435806; ● Search Scopus @ Elsevier (PMID): 18435806; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2008.03647.x

(DOI: 10.1111/j.1365-2249.2008.03647.x, PubMed: 18435806) Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Tsuneko Ono, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
The essentiality and involvement of Streptococcus intermedius histone-like DNA-binding protein in bacterial viability and normal growth,
Molecular Microbiology, Vol.68, No.5, 1268-1282, 2008.

(要約): Streptococcus intermedius histone-like DNA-binding protein (Si-HLP) is a homodimeric protein and, conserved with Escherichia coli HU, a well-documented nucleoid-associated protein (NAP). In E. coli, HU plays important roles as both structural and regulatory factors, but it is not essential for E. coli viability. Streptococcal HLP has been found to bind host cells and induce cytokine production, but its physiological role remains poorly defined. In the present study, using gene insertion knockout and tetracycline-regulated antisense RNA expression techniques, we determined whether Si-HLP is essential for bacterial viability and normal growth in S. intermedius. The Si-HLP-downregulated S. intermedius strain showed alterations in its morphology and surface properties. Downregulation of Si-HLP led to an expanded nucleoid to fill the intracellular space. Transcription levels of several genes, including virulence-associated factors, were found to be activated or repressed in the antisense Si-hlp RNA-expressing strain by real-time PCR and reverse-transcription PCR. Collectively, these data suggest that Si-HLP serves as an essential NAP governing the nucleoid architecture and controlling the gene transcription profile in S. intermedius.
(キーワード): Bacterial Proteins / DNA-Binding Proteins / Escherichia coli Proteins / Gene Expression Regulation, Bacterial / Histones / Microbial Viability / Streptococcus intermedius / Transcription, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2958.2008.06232.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18410499; ● Summary page in Scopus @ Elsevier: 2-s2.0-43749117518

(DOI: 10.1111/j.1365-2958.2008.06232.x, PubMed: 18410499, Elsevier: Scopus) 大石美佳, 松尾敬志, 野間隆文 :
高年次学生合宿研修(正課外授業)によるキャリア形成支援教育の効果と可能性についての実践的研究,
大学教育研究ジャーナル, Vol.5, 46-47, 2008年.

(要約): Two days training camp for the fourth year students of dental school was executed in 2006 and 2007.The purpose of this program is to develop good practical dental doctors through the promotion ofcommunication skills. The training camp was held at the YMCA oceanic center in Anan city of TokushimaPrefecture. It began with an oceanic program under relaxed atmosphere, and then, the programscontaining games, lectures, and workshops related to communication skills were carried out. The theme "support for career formation as a medical profession" was added to "promotion of communication skills" in 2007,and then the skills to learn the definition and the practice of the medical team treatment were installed andexecuted.The usefulness of methodology different from the regular course class was verified based on the results,and the possibility of the application in the future curriculum for the career formation was discussed in thepaper.平成18 年度と19 年度に，歯学部4年生に対して1泊2日の合宿研修を正課外授業として実施した．本事業は，コミュニケーション能力の開発を通じてより良き医療人を育成することが目的である．合宿研修場所は徳島県内の海洋センターであった．リラックスした雰囲気で海洋プログラムからスタートし，次いで，コミュニケーションに対する導入的なゲームや講演，そしてワークショップにより自分たち自身で問題点を抽出し，さらに解決するための討論を行い，その結果をプレゼンテーションをするという内容であった．平成19 年度の2回目には，医学部と薬学部の協力を得て，実施テーマとして「コミュニケーション能力の育成」に「医療専門職としてのキャリア形成支援」という新たなテーマを加え，チーム医療の定義や実践を学ぶ機会を設けて実施した．従来の正課授業と異なった教育方法の可能性について成果を基に検証し，今後のキャリア形成支援教育への応用の可能性について考察した．
(キーワード): 大学教育 / 合宿研修 / コミュニケーション能力 (communication skill) / キャリア形成支援
(徳島大学機関リポジトリ): ● Metadata: 82674
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050001337463779072

(徳島大学機関リポジトリ: 82674, CiNii: 1050001337463779072) Dali Liu, Hiromichi Yumoto, Katsuhiko Hirota, Keiji Murakami, Kanako Takahashi, Kouji Hirao, Takashi Matsuo, Kazuto Ohkura, Hideaki Nagamune and Yoichiro Miyake :
Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via action of ERK1/2 and JNK pathways,
Cellular Microbiology, Vol.10, No.1, 262-276, 2008.

(要約): Streptococcus intermedius is a commensal associated with serious, deep-seated purulent infections in major organs, such as the brain and liver. Histone-like DNA binding protein (HLP) is an accessory architectural protein in a variety of bacterial cellular processes. In this study, we investigated the mechanisms of pro-inflammatory cytokine inductions in THP-1 cells by stimulation with recombinant HLP of S. intermedius (rSi-HLP). rSi-HLP stimulation-induced production of pro-inflammatory cytokines (IL-8, IL-1 beta and TNF-alpha) occurred in a time- and dose-dependent manner. In contrast with the heat-stable activity of DNA binding, the induction activity of rSi-HLP was heat-unstable. In subsequent studies, rSi-HLP acted cooperatively with lipoteichoic acid, the synthetic Toll-like receptor 2 agonist, Pam3CSK4, and the cytosolic nucleotide binding oligomerization domain 2 receptor agonist, muramyldipeptide. Furthermore, Western blot and blocking assays with specific inhibitors showed that rSi-HLP stimulation induced the activation of cell signal transduction pathways, extracellular signal-regulated kinase 1/2 (ERK1/2) and c-Jun N-terminal kinase (JNK). In addition to its physiological role in bacterial growth through DNA binding, these results indicate that Si-HLP can trigger a cascade of events that induce pro-inflammatory responses via ERK1/2 and JNK signal pathways, and suggest that bacterial HLP may contribute to the activation of host innate immunity during bacterial infection.
(キーワード): Acetylmuramyl-Alanyl-Isoglutamine / Bacterial Proteins / Cell Line / Cytokines / DNA, Bacterial / DNA-Binding Proteins / Hot Temperature / Humans / JNK Mitogen-Activated Protein Kinases / Lipopolysaccharides / Mitogen-Activated Protein Kinase 3 / Molecular Sequence Data / Monocytes / Recombinant Proteins / Sequence Analysis, DNA / Signal Transduction / Streptococcus intermedius / Teichoic Acids / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1462-5822.2007.01040.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17883418; ● Search Scopus @ Elsevier (PMID): 17883418; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1462-5822.2007.01040.x

(DOI: 10.1111/j.1462-5822.2007.01040.x, PubMed: 17883418) Tomohiko Adachi, Tadashi Nakanishi, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Kayo Mukai, Hideaki Nakae and Takashi Matsuo :
Caries-related bacteria and cytokines induce CXCL10 in dental pulp.,
Journal of Dental Research, Vol.86, No.12, 1217-1222, 2007.

(要約): Marked infiltration of inflammatory cells, such as activated T-cells, is observed in the progression of pulpitis; however, little is known about the mechanism of their recruitment into pulpal lesions. It has been recently demonstrated that CXC chemokine ligand 10 (CXCL10) chemoattracts CXC chemokine receptor 3 (CXCR3)-positive activated T-cells. We therefore examined whether CXCL10 is involved in the pathogenesis of pulpitis. CXCL10 mRNA expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Immunostaining results revealed that CXCL10 was detected in macrophages, endothelial cells, and fibroblasts in inflamed dental pulp, and that CXCR3 expression was observed mainly on T-cells. Moreover, cultured dental pulp fibroblasts produced CXCL10 after stimulation with live caries-related bacteria, peptidoglycans, and pro-inflammatory cytokines. In contrast, heat-killed bacteria did not induce CXCL10 secretion. These findings suggest that CXCL10-CXCR3 may play an important role in the pulpal immune response to caries-related bacterial invasion. Abbreviations: CXCL10, CXC chemokine ligand 10; CXCR3, CXC chemokine receptor 3; IFN, interferon; FBS, fetal bovine serum; LTA, lipoteichoic acid; PGN, peptidoglycan; IL, interleukin; TNF, tumor necrosis factor; PBS, phosphate-buffered saline; ELISA, enzyme-linked immunosorbent assay; CCL, C-C chemokine ligand; TLR, Toll-like receptor; NOD, nucleotide oligomerization domain; HDPF, human dental pulp fibroblasts.
(キーワード): Adult / Bacteroides / Chemokine CXCL10 / Dental Caries / Dental Pulp / Fibroblasts / Humans / Immunohistochemistry / Lymphocyte Subsets / Middle Aged / RNA, Messenger / Receptors, CXCR3
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/154405910708601215
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18037659; ● Search Scopus @ Elsevier (PMID): 18037659; ● Search Scopus @ Elsevier (DOI): 10.1177/154405910708601215

(DOI: 10.1177/154405910708601215, PubMed: 18037659) Chihiro Shinohara, Kikuji Yamashita, Takashi Matsuo, Seiichiro Kitamura and Fumiaki Kawano :
Effects of Carbonic AnhydraseInhibitor Acetazolamide(AZ)on Osteoclasts and Bone Structure,
Journal of Hard Tissue Biology, Vol.16, No.3, 115-123, 2007.

(要約): We examined the changes of osteoclasts and bone structure in vivo and in vitro under the condition of low activity of bone resorption induced by acetazolamide (AZ). AZ is a specific inhibitor of carbonic anhydrase II (CA II), which is the enzyme indispensable for substantial proton generation by osteoclasts. In vivo study, we investigated histologically and histomorphometrically the changes of trabecular bone and osteoclasts at the cartilage-bone junction of tibiae in rats injected subcutaneously with AZ for 21 days. The area of trabecular bone of AZ treated group increased compared with control group. Moreover, AZ treatment decreased about 20% the number of osteoclasts at the cartilage-bone junction. In vitro study, we examined the effects of AZ on osteoclasts using culture system of rat bone marrow. The number of osteoclasts on the bone slice was suppressed by AZ of a concentration from 10^<-5> to 10^<-4> M in a doze-dependent manner. The number of osteoclasts cultured with 10^<-4>M AZ for 24 hours and with or without parathyroid hormone (PTH) on the bone slice and on the glass showed a significant decrease compared with the control group. Some osteoclasts treated with AZ showed morphological changes of apoptosis-like cell death, including shrinking of cytoplasm and fragmentation of nuclei. These results show that high-doze of AZ decreases bone resorption not only by inhibiting CA II but also by decreasing the number of osteoclasts via induction of cell death. This suggests that CA II plays an important role not only in proton formation but also in osteoclast survival.
(キーワード): Acetazolamide / Bone / Carbonic anhydrase II / Osteoclast
(出版サイトへのリンク): ● Publication site (DOI): 10.2485/jhtb.16.115
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204458174848; ● Search Scopus @ Elsevier (DOI): 10.2485/jhtb.16.115

(DOI: 10.2485/jhtb.16.115, CiNii: 1390001204458174848) Taro Muto, Keiko Miyoshi, Seiichi Munesue, Hiroshi Nakada, Minoru Okayama, Takashi Matsuo and Takafumi Noma :
Differential expression of syndecan isoforms during mouse incisor amelogenesis.,
The Journal of Medical Investigation : JMI, Vol.54, No.3-4, 331-339, 2007.

(要約): Syndecans are transmembranous heparan sulfate proteoglycans (HSPGs) with covalently attached glycosaminoglycan side-chains located on the cell surface. The mammalian syndecan family is composed of four types of syndecans (syndecan-1 to -4). Syndecans interact with the intracellular cytoskeleton through the cytoplasmic domains of their core proteins and membrane proteins, extracellular enzymes, growth factors, and matrix components, through their heparan-sulfate chains, to regulate developmental processes.Here, as a first step to assess the possible roles of syndecan proteins in amelogenesis, we examined the expression patterns of all syndecan isoforms in continuously growing mouse incisors, in which we can overview major differentiation stages of amelogenesis at a glance. Understanding the expression domain of each syndecan isoform during specific developmental stages seems useful for investigating their physiological roles in amelogenesis. Immunohistochemical analysis of syndecan core proteins in the lower incisors from postnatal day 1 mice revealed spatially and temporally specific expression patterns, with syndecan-1 expressed in undifferentiated epithelial and mesenchymal cells, and syndecan-2, -3, and -4 in more differentiated cells. These findings suggest that each syndecan isoform functions distinctly during the amelogenesis of the incisors of mice.
(キーワード): Amelogenesis / Animals / Animals, Newborn / Base Sequence / DNA Primers / Gene Expression Regulation, Developmental / 免疫組織化学 (immunohistochemistry) / Incisor / ノックアウトマウス (knockout mice) / Mice, Inbred ICR / Protein Isoforms / Syndecans
(徳島大学機関リポジトリ): ● Metadata: 111537
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.54.331
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17878683; ● Summary page in Scopus @ Elsevier: 2-s2.0-35848960794

(徳島大学機関リポジトリ: 111537, DOI: 10.2152/jmi.54.331, PubMed: 17878683, Elsevier: Scopus) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
CXC chemokine ligand 16 in periodontal diseases: expression in diseased tissues and production by cytokine-stimulated human gingival fibroblasts.,
Clinical and Experimental Immunology, Vol.149, No.1, 146-154, 2007.

(要約): Periodontal disease is an inflammatory disorder characterized by the involvement of chemokines that are important for the recruitment of leucocytes. Several cytokines are involved in regulating levels of chemokines in periodontal disease. CXCL16 is a chemokine related to the migration of T helper 1 (Th1) cells and natural killer (NK) cells. In this study, we examined its expression in periodontal tissues. Moreover, we investigated the effects of cytokines on the production of CXCL16 by human gingival fibroblast (HGF). Reverse transcription-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry revealed that CXCL16 and its receptor, CXCR6, were expressed at the mRNA and protein levels in diseased tissues. Proinflammatory cytokines [interleukin (IL)-1beta, tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma] increased the mRNA expression and release of CXCL16 in a dose-dependent manner. Moreover, treatment of HGFs with IFN-gamma in combination with IL-1beta had a synergistic effect on the production of CXCL16. On the other hand, IL-4 and IL-13 inhibited the IL-1beta-induced CXCL16 production by HGFs. Inhibitors of A disintegrin and metalloprotease (ADAM)10 and ADAM17, a recently identified protease of CXCL16, reduced the amount of CXCL16 released from HGFs. These results suggest that the CXCL16 produced by HGFs may be involved in the migration of leucocytes into inflamed tissues, and provide evidence that CXCL16 production is controlled by cytokines in periodontal disease.
(キーワード): Aged / Cells, Cultured / Chemokines, CXC / Chronic Disease / Cytokines / Female / Fibroblasts / Gene Expression / Gingiva / Humans / Interferon-gamma / Interleukin-13 / Interleukin-1beta / Interleukin-4 / Male / Metalloproteases / Middle Aged / Mitogen-Activated Protein Kinases / Periodontitis / RNA, Messenger / Receptors, Chemokine / Receptors, Scavenger / Receptors, Virus / Reverse Transcriptase Polymerase Chain Reaction
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2007.03398.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17459077; ● Search Scopus @ Elsevier (PMID): 17459077; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2007.03398.x

(DOI: 10.1111/j.1365-2249.2007.03398.x, PubMed: 17459077) 菅俊行, 石川邦夫, 松尾敬志, 恵比須繁之 :
フッ化ジアミンシリケートの象牙質知覚過敏症治療剤への応用―抜去歯を用いた象牙細管封鎖能の検討―,
日本歯科保存学雑誌, Vol.50, No.3, 313-320, 2007年.

(要約): フッ化ジアンミン銀(サホライド^[○!R])は,齲蝕進行抑制剤および象牙質知覚過敏症治療剤として臨床で使用されている.しかしながら,フッ化ジアンミン銀は塗布後に銀の沈着による歯の黒変が起こる.したがって,フッ化ジアンミン銀は主に乳歯に使用されている.この欠点を改良するために,フッ化ジアミンシリケートを調製した.銀の代わりにシリカを導入した理由は,シリカは歯質の変色を起こさないこと,そして擬似体液からアパタイトの生成を誘導するからである.本研究の目的はヒト口腔内を模倣した環境下において,フッ化ジアミンシリケート処理後の象牙細管封鎖効果と持続性を評価することである.フッ化ジアミンシリケートの象牙細管封鎖効果は,ヒト抜去歯を用いて評価を行った.フッ化ジアミンシリケート処理直後および人工唾液浸漬7日後の象牙質プレートの表面を,走査電子顕微鏡(SEM)にて観察を行った.SEM観察の結果,開口象牙細管はフッ化ジアミンシリケート処理直後にはシリカ-リン酸カルシウム結晶により完全に封鎖されていた.さらに,人工唾液浸漬7日後には象牙質表面は新たに生成した結晶により覆われていた.EDXA分析によると,フッ化ジアミンシリケート処理直後に象牙細管内に析出した結晶はシリカ,カルシウム,リンを含有しており,シリカ-リン酸カルシウム結晶であることが明らかとなった.その結晶のカルシウムとリンのモル比はフッ化ジアミンシリケート処理直後には2.02であったが,人工唾液浸漬後には徐々に減少した.一方,象牙質表面に新たに生成した結晶は実験期間を通して,ほぼ一定の値(Ca/P=1.16∼1.28)であった.周囲の管間象牙質と比べて有意に低い値であることから,おそらくカルシウム欠損アパタイトが析出していると推察された.フッ化ジアミンシリケート処理は人工唾液中からリン酸カルシウムの析出を誘導し,したがって,ヒト口腔内を模倣した環境下において,持続的な象牙細管封鎖能を有することが明らかとなった.
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205520321280

(CiNii: 1390001205520321280) Hiromichi Yumoto, Masayoshi Yamada, Chihiro Shinohara, Hideaki Nakae, Kanako Takahashi, Hiroyuki Azakami, Ebisu Shigeyuki and Takashi Matsuo :
Soluble products from Eikenella corrodens induce cell proliferation and expression of interleukin-8 and adhesion molecules in endothelial cells via mitogen-activated protein kinase pathways,
Oral Microbiology and Immunology, Vol.22, No.1, 36-45, 2007.

(要約): The periodontal vasculature is profoundly affected during the progression of periodontitis, and several specific bacteria are believed to be involved in this inflammatory disease. Eikenella corrodens is one of the common bacteria detected in periodontitis diseased lesions; however, the function of this organism in periodontitis is not well understood. In this study, we investigated the E. corrodens-induced endothelial cell alteration and inflammation process that leads to leukocyte infiltration in inflamed regions. Soluble products from E. corrodens (EcSP) induced the gene expression and protein production of vascular endothelial growth factor in oral epithelial cells and human umbilical vein endothelial cells (HUVEC). Direct stimulation by EcSP also activated endothelial cell proliferation. Moreover, EcSP induced ERK1/2 (p44/42) and p38 mitogen-activated protein kinase (MAPK) phosphorylation within 10-30 min in HUVEC, as demonstrated by Western blot analysis and up-regulated intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), E-selectin and interleukin-8 (IL-8) production demonstrated by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. The specific p38 MAPK inhibitor SB203580 reduced the expression of ICAM-1, VCAM-1 and IL-8, whereas the blockade of p44/42 by MAPK kinase (MEK1) inhibitor, PD98059, inhibited only IL-8 expression. Our results indicate that E. corrodens can trigger a cascade of events that induce inflammatory responses in periodontal tissue via the MAPK cascade and may promote chronic periodontitis without bacteria-cell contact.
(キーワード): Cell Adhesion Molecules / Cell Line / Cell Proliferation / Cells, Cultured / Culture Media, Conditioned / E-Selectin / Eikenella corrodens / Endothelial Cells / Endothelium, Vascular / Enzyme Inhibitors / Epithelial Cells / Flavonoids / Humans / Imidazoles / Intercellular Adhesion Molecule-1 / Interleukin-8 / KB Cells / Mitogen-Activated Protein Kinase 1 / Mitogen-Activated Protein Kinase 3 / Mitogen-Activated Protein Kinases / Periodontitis / Phosphorylation / Pyridines / Vascular Cell Adhesion Molecule-1 / Vascular Endothelial Growth Factor A / p38 Mitogen-Activated Protein Kinases
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1399-302X.2007.00320.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17241169; ● Search Scopus @ Elsevier (PMID): 17241169; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1399-302X.2007.00320.x

(DOI: 10.1111/j.1399-302X.2007.00320.x, PubMed: 17241169) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Proinflammatory effects of tumour necrosis factor-like weak inducer of apoptosis (TWEAK) on human gingival fibroblasts.,
Clinical and Experimental Immunology, Vol.146, No.3, 540-549, 2006.

(要約): Tumour necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK), a member of the TNF family, is a multi-functional cytokine that regulates cellular proliferation, angiogenesis, inflammation and apoptosis. In this study, we investigated TWEAK expression in periodontally diseased tissues and the effect of TWEAK on human gingival fibroblasts (HGF). Reverse transcription-polymerase chain reaction (RT-PCR) analysis and immunohistochemistry revealed that TWEAK and the TWEAK receptor, fibroblast growth factor-inducible 14 (Fn14), mRNA and protein were expressed in periodontally diseased tissues. HGF expressed Fn14 and produced interleukin (IL)-8 and vascular endothelial growth factor (VEGF) production upon TWEAK stimulation in a dose-dependent manner. The IL-8 and VEGF production induced by TWEAK was augmented synergistically by simultaneous stimulation with transforming growth factor (TGF)-beta1 or IL-1beta. IL-1beta and TGF-beta1 enhanced Fn14 expression in a dose-dependent manner. Moreover, TWEAK induced intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression on HGF in a dose-dependent manner. The ICAM-1 expression induced by TWEAK was augmented by TGF-beta1. On the other hand, the TWEAK-induced VCAM-1 expression was inhibited by TGF-beta1. Phosphatidylinositol 3-kinase (PI3K) and nuclear factor-kappaB (NF-kappaB) inhibitor inhibit both ICAM-1 and VCAM-1 expression induced by TWEAK. However, mitogen-activated protein kinase (MEK) and c-Jun NH2-terminal kinase (JNK) inhibitor enhanced only VCAM-1 expression on HGF. These results suggest that TWEAK may be involved in the pathophysiology of periodontal disease. Moreover, in combination with IL-1beta or TGF-beta1, TWEAK may be related to the exacerbation of periodontal disease to induce proinflammatory cytokines and adherent molecules by HGF.
(キーワード): Adult / Aged / Aged, 80 and over / Apoptosis / Cells, Cultured / Dose-Response Relationship, Immunologic / Female / Fibroblasts / Gene Expression / Gingiva / Humans / Intercellular Adhesion Molecule-1 / Interleukin-1beta / Interleukin-8 / Male / Middle Aged / Periodontitis / RNA, Messenger / Receptors, Tumor Necrosis Factor / Reverse Transcriptase Polymerase Chain Reaction / Signal Transduction / Transforming Growth Factor beta1 / Tumor Necrosis Factors / Vascular Cell Adhesion Molecule-1 / Vascular Endothelial Growth Factor A
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2006.03233.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17100776; ● Search Scopus @ Elsevier (PMID): 17100776; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2006.03233.x

(DOI: 10.1111/j.1365-2249.2006.03233.x, PubMed: 17100776) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Cytokines differentially regulate ICAM-1 and VCAM-1 expression on human gingival fibroblasts.,
Clinical and Experimental Immunology, Vol.144, No.3, 494-502, 2006.

(要約): The expression of intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) on human gingival fibroblasts (HGF) may be important for migration and retention of inflammatory cells in periodontally diseased tissue. This study aimed to assess which cytokines regulate ICAM-1 and VCAM-1 expression on HGF. Tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma enhanced both ICAM-1 and VCAM-1 expression on HGF. Interleukin (IL)-1beta mainly up-regulated ICAM-1 expression. On the other hand, IL-4 and IL-13 enhanced only VCAM-1 expression on HGF. IL-10 did not modulate both ICAM-1 and VCAM-1 expression. Transforming growth factor (TGF)-beta1 enhanced ICAM-1 expression. However, TGF-beta1 inhibited the VCAM-1 expression induced by TNF-alpha or IL-4. Both ICAM-1 and VCAM-1 expression by HGF was inhibited by nuclear factor-kappaB (NF-kappaB) activation inhibitor (MG-132). Mitogen-activated protein kinases (MAPK) inhibitors did not influence ICAM-1 expression induced by TNF-alpha. Interestingly, VCAM-1 expression was enhanced by MEK inhibitor (PD98059) and c-Jun NH2-terminal kinase (JNK) inhibitor (SP600125). These results mean that the balance of cytokines in periodontally diseased tissue may be essential for control of ICAM-1 and VCAM-1 expression on HGF, and the balance of ICAM-1 and VCAM-1 expression might be important for regulation of leucocytes infiltration and retention in periodontally diseased tissue.
(キーワード): Cells, Cultured / Cytokines / Drug Synergism / Enzyme Inhibitors / Fibroblasts / Flow Cytometry / Gene Expression Regulation / Gingiva / Humans / Imidazoles / Intercellular Adhesion Molecule-1 / Leupeptins / Mitogen-Activated Protein Kinase Kinases / NF-kappa B / Pyridines / RNA, Messenger / Recombinant Proteins / Reverse Transcriptase Polymerase Chain Reaction / Vascular Cell Adhesion Molecule-1
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2006.03064.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16734619; ● Search Scopus @ Elsevier (PMID): 16734619; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2006.03064.x

(DOI: 10.1111/j.1365-2249.2006.03064.x, PubMed: 16734619) Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and Shigeyuki Ebisu :
Effect of ammonium hexafluorosilicate on dentin tubule occlusion for the treatment of dentin hypersensitivity,
American Journal of Dentistry, Vol.19, No.4, 248-252, 2006.

(要約): To evaluate the occluding ability of ammonium hexafluorosilicate (SiF). Dentin disks prepared from human extracted teeth were grouped as follows to prepare different situations of dentin hypersensitivity: (1) those sonicated for 20 minutes; (2) those treated with 0.5 mol/L EDTA for 2 minutes; (3) those treated with 6% citric acid for 2 minutes; (4) those treated with 50% citric acid for 2 minutes. Then, SiF or diamine silver fluoride (AgF) was applied to the dentin disks and the dentin tubule occlusion was observed with scanning electron microscopy (SEM). The percent of open tubules before and after SiF or AgF treatment were measured by NIH image using SEM photographs. Also, the dentin permeability was measured. SEM micrographs demonstrated that the dentin tubules were completely occluded by the precipitate after SiF treatment. Also, the dentin permeability was reduced to 10.3%. In contrast, most of the dentin tubules remained open after AgF treatment. EDXA analysis showed that the precipitate in the dentin tubules that forms after SiF treatment contains Si, Ca and P, indicating a silica-calcium phosphate complex.
(キーワード): Acids / Adult / Analysis of Variance / Citric Acid / Dentin / Dentin Permeability / Dentin Sensitivity / Edetic Acid / Fluorides / Humans / Silicic Acid
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16939033; ● Search Scopus @ Elsevier (PMID): 16939033

(PubMed: 16939033) Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and Shigeyuki Ebisu :
Effects of plaque control on the patency of dentinal tubules-an in vivo study in beagle dogs,
Journal of Periodontology, Vol.77, No.3, 454-459, 2006.

(要約): The aim of this in vivo study was to evaluate the effects of plaque control on the patency of dentinal tubules using vital teeth of beagle dogs. Class V cavities were prepared on the cervical areas of the mandibular and maxillary molars in each dog with a diamond point. To simulate the state of dentinal hypersensitivity, the teeth were etched with 50% citric acid for 2 minutes to obtain patent dentinal tubules. Plaque control was achieved by brushing the left-side teeth every day, whereas no plaque control was performed for the right-side teeth. A dentin biopsy was performed after 1, 2, and 3 weeks using the cylindrical diamond point to obtain dentin specimens. In the plaque control group, some of the dentinal tubules were occluded with precipitate (Ca/P=1.49), and the diameter of the dentinal tubules decreased from 2.42+/-0.33 microm (mean+/-SD) to 1.11+/-0.51 microm after 7 days, although most of the dentinal tubules remained open. In contrast, no precipitate was observed in the dentinal tubules of the non-plaque control group. Also, the diameter of the dentinal tubules increased from 2.42+/-0.33 to 2.9+/-0.49 microm, due to the demineralization of the peritubular and intertubular dentin. Plaque control plays a key role in reducing the patency of dentinal tubules and, therefore, might promote the natural repair of dentinal hypersensitivity.
(キーワード): Analysis of Variance / Animals / Calcium Phosphates / Dental Plaque / Dentin / Dentin Permeability / Dentin Sensitivity / Dogs / Electron Probe Microanalysis / Microscopy, Electron, Scanning / Toothbrushing
(出版サイトへのリンク): ● Publication site (DOI): 10.1902/jop.2006.050159
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16512760; ● Summary page in Scopus @ Elsevier: 2-s2.0-33646346136

(DOI: 10.1902/jop.2006.050159, PubMed: 16512760, Elsevier: Scopus) Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Takashi Matsuo and SHigeyuki Ebisu :
Comparison of the occluding ability of dentinal tubules with different morphology between calcium phosphate precipitation method and potassium oxalate treatment,
Dental Materials Journal, Vol.24, No.4, 522-529, 2005.

(要約): The aim of this study was to evaluate the occluding ability of calcium phosphate precipitation (CPP) method and potassium oxalate treatment when each method was applied to dentin disks with different surface morphology. Occluding ability was observed by scanning electron microscopy. Irrespective of the diameter of the dentinal tubules, the CPP method showed a consistent occluding ability for dentinal tubules at the dentin surface, and that the depths of the precipitate formed in the dentinal tubules by CPP method were not significantly different. In contrast, the occluding ability of potassium oxalate treatment was reduced with increasing diameter of the dentinal tubules. However, the reduction of the occluding ability of potassium oxalate treatment was more markedly affected by the demineralization of dentin surface. Since the CPP method showed a consistent occluding ability irrespective of the diameter of the dentinal tubules, it is suggested that the CPP method would be a useful means for treating dentin hypersensitivity.
(キーワード): Adult / Calcium Phosphates / Dentin / Dentin Permeability / Dentin Sensitivity / Humans / Microscopy, Electron, Scanning / Oxalates / Surface Properties
(出版サイトへのリンク): ● Publication site (DOI): 10.4012/dmj.24.522
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16445013; ● Search Scopus @ Elsevier (PMID): 16445013; ● Search Scopus @ Elsevier (DOI): 10.4012/dmj.24.522

(DOI: 10.4012/dmj.24.522, PubMed: 16445013) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Increase of CCL20 expression by human gingival fibroblasts upon stimulation with cytokines and bacterial endotoxin,
Clinical and Experimental Immunology, Vol.142, No.2, 285-291, 2005.

(要約): We have demonstrated recently that CCL20 was expressed in periodontal diseased tissues and abundant CCR6 positive T cells infiltrated in periodontally diseased tissue. However, it is uncertain which cells can elicit CCL20 production. In the present study, we examined the properties of CCL20 production by human gingival fibroblasts (HGF) culture. Here, we report that interleukin-1 beta (IL-1beta), tumour necrosis factor-alpha (TNF-alpha) and Escherichia coli lipopolysaccharide (LPS) can significantly induce the production of CCL20 by HGF. We found that TNF-alpha and E. coli LPS enhanced the production of CCL20 by HGF treated with IL-1beta. In contrast, interferon-gamma (IFN-gamma) dramatically diminished CCL20 production induced by IL-1beta. Moreover, we demonstrated that nuclear factor-kappaB (NF-kappaB), p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinases (ERK) play an important role in mediating the production of CCL20 induced by IL-1beta and TNF-alpha. On the other hand, we found that not only NF-kappaB, p38 MAPK and ERK but also c-Jun NH2-terminal kinase (JNK) are involved in CCL20 production induced by E. coli LPS. Finally, we found that HGF express CCR6, CCL20 receptor, and CCL20 induced vascular endothelial growth factor (VEGF) by HGF. Taken together, these findings that HGF will be a source of CCL20 in periodontal tissue, and the CCL20 production will be controlled by proinflammatory cytokine and bacterial LPS in periodontally diseased tissue. Thus, CCL20 by HGF might be involved in inflammatory cells infiltration, and promote the progression of periodontal disease.
(キーワード): CCL20 / fibroblast / 歯周炎 (periodontal disease)
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2005.02912.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16232215; ● Search Scopus @ Elsevier (PMID): 16232215; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2005.02912.x

(DOI: 10.1111/j.1365-2249.2005.02912.x, PubMed: 16232215) Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae, Keiji Murakami, Yoichiro Miyake and Takashi Matsuo :
CXCL12 and CXCR4 expression by human gingival fibroblasts in periodontal disease,
Clinical and Experimental Immunology, Vol.141, No.3, 467-474, 2005.

(要約): CXCL12 is a CXC chemokine that is related to lymphocyte infiltration and angiogenesis in inflammatory sites such as arthritis. However, the expression and roles of CXCL12 in periodontal disease are uncertain. The aim of this study was to assess the expression of CXCL12 and its receptor, CXCR4, in periodontal tissue and to investigate the properties of CXCL12 and CXCR4 expression by human gingival fibroblasts (HGF). RT-PCR analysis revealed that CXCL12 and CXCR4 mRNA were expressed in both normal gingival tissues and periodontal diseased tissues. Immunohistochemistry disclosed that CXCL12 was expressed and CXCR4 positive cells were found in both normal and periodontal diseased gingival tissues. Our in vitro experiments elucidated that HGF constitutively produced CXCL12, and the levels were enhanced by stimulation with tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), transforming growth factor-beta (TGF-beta), regulated upon activation normal T cell expressed and secreted (RANTES) and macrophage inflammatory protein 3(alpha) (MIP-3(alpha)). On the other hand, heat killed Porphyromonas gingivalis (P. gingivalis) and P. gingivalis LPS reduced the CXCL12 production by HGF. Flow cytometry analysis clarified that CXCR4 was highly expressed on HGF, and CXCR4 expression was abrogated by TNF-alpha, IFN-gamma and P. gingivalis LPS. Moreover, CXCL12 induced vascular endothelial growth factor (VEGF) production by HGF. Our results demonstrated that CXCL12 might be related to CXCR4+ cells infiltration and angiogenesis both in normal periodontal tissues and periodontal diseased tissue. P. gingivalis, a known periodontal pathogen, inhibits the production of CXCL12 and the expression of CXCR4 by HGF. This fact means that P. gingivalis may inhibit CXCR4+ cells infiltration and neovascularization in periodontal tissue and escape from the immune response.
(キーワード): CXCL12 / CXCR4 / fibroblast / 歯周炎 (periodontal disease) / Porphyromonas gingivalis
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2005.02852.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16045736; ● Search Scopus @ Elsevier (PMID): 16045736; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2005.02852.x

(DOI: 10.1111/j.1365-2249.2005.02852.x, PubMed: 16045736) Akiko Kawasaki, Toshiyuki Suge, Kunio Ishikawa, Kazumi Ozaki, Takashi Matsuo and Shigeyuki Ebisu :
Ammonium hexafluorosilicate increase acid resistance of bovine enamel and dentine,
Journal of Materials Science. Materials in Medicine, Vol.16, No.5, 461-466, 2005.

(要約): Although diamine silver fluoride (AgF: (NH3)2AgF) stains teeth black, it is known as a very effective agent to prevent the dental caries progress. In order to find another fluoride that has a similar anticariogenic effect without changing tooth color, we prepared ammonium hexafluorosilicate (SiF: (NH4)2SiF6), in which the silver of AgF is replaced with silicon. In this study, the anticariogenic effect of SiF was evaluated using bovine teeth. Fluoride solutions, SiF, AgF, acidulated phosphate fluoride (APF), and sodium fluoride (NaF), were applied to bovine enamel and dentine blocks, and the depth of demineralization was measured after exposure to a demineralizing solution for 24 h. Also, fluoride was applied to a simulated dentine caries specimen to evaluate the caries progress-preventing ability. For the dentine specimens, mineral loss (Delta Z) was also measured with microradiography. We found that SiF treated enamel showed better acid resistance than specimens treated with NaF or APF. AgF treated enamel also showed similar acid resistance, but was stained black. SiF and AgF treated caries-affected dentine showed reduced demineralization when exposed to a demineralization solution for 24 h. Mineral loss (Delta Z) was reduced to 85% and 75%, respectively. Although the acid resistance of the SiF treated teeth was inferior to that of the AgF treated teeth, we consider that SiF has good potential as anticariogenic agent, since it increased acid resistance without changing tooth color.
(キーワード): Acids / Animals / Biocompatible Materials / Calcification, Physiologic / Cattle / Dental Caries / Dental Enamel / Dentin / Drug Resistance / Fluorides / Hydrogen-Ion Concentration / Incisor / Silicic Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10856-005-6987-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15875257; ● Search Scopus @ Elsevier (PMID): 15875257; ● Search Scopus @ Elsevier (DOI): 10.1007/s10856-005-6987-2

(DOI: 10.1007/s10856-005-6987-2, PubMed: 15875257) Toshiyuki Suge, Ishikawa Kunio, Akiko Kawasaki, Takashi Matsuo and EBISU Shigeyuki :
Effects of pre- or post- application with calcium chloride on occluding ability of potassium oxalate for the treatment of dentin hypersensitivity,
American Journal of Dentistry, Vol.18, No.2, 121-125, 2005.

(要約): To evaluate whether calcium ion supply using 1-6 mol/L CaCl2 solution could enhance the occluding ability of dentin tubules with 30% potassium oxalate treatment. Calcium chloride solution (1-6 mol/L) was applied to dentin disks before or after application of 30% potassium oxalate solution. Occluding ability after potassium oxalate treatment was evaluated with scanning electron microscopic (SEM) observation and measurement of dentin permeability. In addition, the composition of the precipitate formed when mixing potassium oxalate and calcium chloride was analyzed with a powder x-ray diffractometer (XRD). SEM observations revealed that the dentin tubules were occluded homogeneously and completely with the precipitate when calcium chloride solution was applied before or after potassium oxalate treatment. However, the depth of the precipitate in dentin tubules from the dentin surface became shallower when pre-treated with calcium chloride before potassium oxalate. Although dentin permeability was greatly reduced in both groups, no significant difference could be observed between samples with and without calcium chloride application.
(キーワード): Adult / Calcium Chloride / Chemical Precipitation / Dentin / Dentin Permeability / Dentin Sensitivity / Drug Synergism / Humans / Microscopy, Electron, Scanning / Oxalates / X-Ray Diffraction
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15973832; ● Search Scopus @ Elsevier (PMID): 15973832

(PubMed: 15973832) Yoshitaka Hosokawa, Tadashi Nakanishi, Daisuke Yamaguchi, Hideaki Nakae and Takashi Matsuo :
Expression of fractalkine (CX3CL1) and its receptor, CX3CR1, in periodontal diseased tissue,
Clinical and Experimental Immunology, Vol.139, No.3, 506-512, 2005.

(要約): The regulatory role of chemokines and chemokine receptors on specific leucocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that leucocytes infiltrating inflamed gingival tissue expressed marked levels of CX3CR1. In periodontal diseased tissue, the expression of fractalkine and CX3CR1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) and further, fractalkine was distributed mainly on endothelial cells, as shown by immunohistochemistry. Moreover, we can detect CX3CR1-expressing cells infiltrated in periodontal diseased tissue by immunohistochemical staining. Furthermore, fractalkine production by human umbilical vein endothelial cells (HUVEC) was up-regulated by pathogen-associated molecular patterns (PAMPs), including Porphyromonas gingivalis lipopolysaccharide (LPS). Thus, these findings suggested that CX3CR1 and the corresponding chemokine, fractalkine may have an important regulatory role on specific leucocyte migration into inflamed periodontal tissue.
(キーワード): CX3CR1 / fractalkine / 歯周炎 (periodontal disease)
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2249.2005.02675.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15730397; ● Search Scopus @ Elsevier (PMID): 15730397; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2249.2005.02675.x

(DOI: 10.1111/j.1365-2249.2005.02675.x, PubMed: 15730397) Tadashi Nakanishi, Kanako Takahashi, Yoshitaka Hosokawa, Tomohiko Adachi, Hideaki Nakae and Takashi Matsuo :
Expression of macrophage inflammatory protein 3alpha in human inflamed dental pulp tissue,
Journal of Endodontics, Vol.31, No.2, 84-87, 2005.

(要約): Severe pulpitis resulting from dental caries is characterized by marked inflammatory infiltrate such as lymphocytes. Little is known about the recruitment of these cells into the dental pulp lesions of carious teeth. Macrophage inflammatory protein-3alpha (MIP-3alpha), a CC chemokine attracts CC chemokine receptor 6 (CCR6)-expressing T cells. We examined the distribution of MIP-3alpha-positive and/or CCR6-positive cells in human inflamed and normal dental pulp by immunohistochemistry. MIP-3alpha was observed in all inflamed pulp sections, and was mostly distributed in macrophages that had accumulated in the area adjacent to carious lesions. Furthermore, CCR6 expression was also observed in the infiltrating lymphocytes. In contrast, MIP-3alpha and CCR6 were rarely detected in normal pulp. These findings suggest that MIP-3alpha plays a role in the advancement of pulpal inflammation via the recruitment of CCR6-expressing lymphocytes.
(キーワード): Adult / Case-Control Studies / Chemokine CCL20 / Chemokines, CC / Chemotaxis, Leukocyte / Dental Caries / Humans / Immunohistochemistry / Macrophage Inflammatory Proteins / Middle Aged / Pulpitis / Receptors, CCR6 / Receptors, Chemokine / T-Lymphocytes
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/01.don.0000143414.22112.57
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15671814; ● Search Scopus @ Elsevier (PMID): 15671814; ● Search Scopus @ Elsevier (DOI): 10.1097/01.don.0000143414.22112.57

(DOI: 10.1097/01.don.0000143414.22112.57, PubMed: 15671814) Takashi Matsuo, Toshiyuki Shirakami, Kazumi Ozaki, Tadashi Nakanishi, Hiromichi Yumoto and Shigeyuki Ebisu :
An immunohistological study of the localization of bacteria invading root pulpal walls of teeth with periapical lesions,
Journal of Endodontics, Vol.29, No.3, 194-200, 2003.

(要約): We immunohistologically examined the prevalence and localization of bacteria invading dentinal tubules of the roots of teeth with infected canals. Forty extracted teeth with apical lesions were selected and divided into two groups: a group of untreated teeth and a group of canal-enlarged teeth. The bacteria in the specimens were detected by Brown-Brenn stain and the labeled-streptavidin-biotin method with specific antisera for 16-bacteria. Seventy percent of the examined teeth showed bacteria invading the dentinal tubules of the roots. Fusobacterium nucleatum, Eubacterium alactolyticum, E. nodatum, Lactobacillus casei, and Peptostreptococcus micros were abundant. Even in the canal-enlarged group, invasion of bacteria was observed in 65% of teeth. This study revealed the actual condition of bacteria in infected root dentin and suggested that the canal-enlargement procedure could not completely remove all the bacteria in the infected dentinal tubules of the root.
(キーワード): Adult / Aged / Bacteria / Coloring Agents / Dental Pulp / Dentin / Eubacterium / Fusobacterium nucleatum / Humans / Immune Sera / Immunoenzyme Techniques / Immunohistochemistry / Lactobacillus casei / Middle Aged / Peptostreptococcus / Periapical Diseases / Porphyromonas / Prevotella / Root Canal Preparation / Statistics, Nonparametric / Tooth Root
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00004770-200303000-00008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12669880; ● Search Scopus @ Elsevier (PMID): 12669880; ● Search Scopus @ Elsevier (DOI): 10.1097/00004770-200303000-00008

(DOI: 10.1097/00004770-200303000-00008, PubMed: 12669880) Masayoshi Yamada, Hideaki Nakae, Hiromichi Yumoto, Chihiro Shinohara, Shigeyuki Ebisu and Takashi Matsuo :
N-acetyl-D-galactosamine specific lectin of Eikenella corrodens induces intercellular adhesion molecule-1 (ICAM-1) production by human oral epithelial cells,
Journal of Medical Microbiology, Vol.51, No.12, 1080-1089, 2002.

(要約): During the acute inflammatory response in periodontitis, gingival epithelial cells are considered to play important roles in the recruitment of inflammatory cells to the site of infection through the secretion of chemokines. However, little is known about the expression of molecules that are involved in the interaction between the epithelium and neutrophils following bacterial attachment. Earlier work reported that periodontopathogenic Eikenella corrodens strain 1,073 up-regulated the expression and secretion of chemokines such as interleukin-8 (IL-8) from KB cells (a human oral epithelial cell line derived from a human oral epidermoid carcinoma). To elucidate the mechanism of the transmigration of neutrophils through the epithelium, the present study investigated the expression of adhesion molecules on KB cells in response to E. corrodens attachment. Adhesion molecule gene expression was assessed by RT-PCR and adhesion proteins expressed on KB cell surfaces were determined by cell-based ELISA and FACS. In RT-PCR, ICAM-1 mRNA levels were significantly increased within 1 h in response to exposure to E. corrodens and continued to increase over the 12-h period of study. In ELISA, increased surface ICAM-1 expression was paralleled by increased ICAM-1 mRNA levels. Furthermore, the increases in ICAM-1 expression on epithelial cells infected with E. corrodens were observed to be due to the N-acetyl-D-galactosamine (GalNAc) specific bacterial lectin-like substance of E. corrodens (EcLS), which was one of the adhesins of E. corrodens. This is the first study to report that a bacterial lectin-like substance increased the expression of ICAM-1 on gingival epithelial cells.
(キーワード): レクチン / ICAM-1 / Eikenella corrodens / 上皮細胞
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12466406; ● Search Scopus @ Elsevier (PMID): 12466406

(PubMed: 12466406) Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Kazuomi SUZUKI, Takashi Matsuo, Yuichiro NOIRI, Satoshi IMAZATO and Shigeyuki EBISU :
Calcium phosphate precipitation method for the treatment of dentin hypersensitivity,
American Journal of Dentistry, Vol.15, No.4, 220-226, 2002.

(要約): To evaluate the feasibility of the calcium phosphate precipitation (CPP) method as a treatment for dentin hypersensitivity using vital teeth of beagle dogs. Also, gingival tissue response to two types of CPP methods was examined histologically. Dentin tubules of the dogs' vital teeth were exposed by shallow cavity preparation followed by etching with 50% citric acid to simulate the condition of hypersensitive dentin. After CPP treatment was applied to the vital tooth, the dentin surface and longitudinal sections were observed by SEM to evaluate the occluding ability of the CPP method. The precipitate was also analyzed by energy-dispersive X-ray microanalysis to obtain compositional information. The gingival tissue before and after CPP method was examined histologically with light microscopy. Dentin tubules were occluded homogeneously and completely with an apatitic mineral after application of the CPP treatment in vital teeth. However, the depth of the precipitate in dentin tubules from the dentin surface was approximately half that seen in extracted teeth. No histological change was observed in gingival tissues when NaHCO3 was used as a post-treatment solution of the CPP method, whereas another CPP method using NaOH solution resulted in atrophy and degeneration of the epithelium of gingival tissue.
(キーワード): Analysis of Variance / Animals / Calcium Phosphates / Chemical Precipitation / Dentin / Dentin Permeability / Dentin Sensitivity / Dogs / Electron Probe Microanalysis / Feasibility Studies / Gingiva / Microscopy, Electron, Scanning
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12572638; ● Summary page in Scopus @ Elsevier: 2-s2.0-0037844949

(PubMed: 12572638, Elsevier: Scopus) Yoshitaka Hosokawa, Tadashi Nakanishi, Daisuke Yamaguchi, Kanako Takahashi, Hiromichi Yumoto, Kazumi Ozaki and Takashi Matsuo :
Macrophage Inflammatory Protein 3α-CC chemokine receptor 6 interactions play an important role in CD4⁺ T-cell accumulation in periodontal diseased tissue.,
Clinical and Experimental Immunology, Vol.128, No.3, 548-554, 2002.

(要約): The regulatory role of chemokines and chemokine receptors on specific lymphocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that lymphocytes infiltrating inflamed gingival tissue expressed marked levels of CCR6. In periodontal diseased tissue, the expression of MIP-3alpha mRNA was detected by RT-PCR and further, MIP-3alpha was distributed in the basal layer of gingival epithelial cells, microvascular endothelial cells and the areas of inflammatory cells as shown by immunohistochemistry. Moreover, CCR6-expressing cells infiltrated into periodontal diseased tissue, and the proportion of CCR6-positive CD4+ T cells was significantly elevated in periodontal diseased tissue compared with peripheral blood in the same patients. Furthermore, gingival lymphocytes isolated from patients showed migration toward MIP-3alpha in an in vitro chemotaxis assay in which migration was abrogated by specific antibody to CCR6. Thus, these findings suggested that CCR6 and the corresponding chemokine, MIP-3alpha may have an important regulatory role in specific lymphocyte migration into inflamed periodontal tissue.
(キーワード): CCR6 / MIP-3 alpha / periodontal disease / MONOCYTE CHEMOATTRACTANT PROTEIN-1 / INFLAMED HUMAN GINGIVA / IMMUNOHISTOLOGICAL ANALYSIS / SELECTIVE RECRUITMENT / INDUCIBLE EXPRESSION / BACTERIA / IMMUNITY / HEALTHY / LESIONS / SUBSETS
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.1365-2249.2002.01865.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12067311; ● Search Scopus @ Elsevier (PMID): 12067311; ● Search Scopus @ Elsevier (DOI): 10.1046/j.1365-2249.2002.01865.x

(DOI: 10.1046/j.1365-2249.2002.01865.x, PubMed: 12067311) Hiromichi Yumoto, Hideaki Nakae, Masayoshi Yamada, Keiko Fujinaka, Chihiro Shinohara, Shigeyuki Ebisu and Takashi Matsuo :
Soluble products from Eikenella corrodens stimulate oral epithelial cells to induce inflammatory mediators,
Oral Microbiology and Immunology, Vol.16, No.5, 296-305, 2001.

(要約): In the inflammatory response elicited by bacterial colonization in periodontal pockets, pocket epithelial cells not only serve as a barrier to isolate the pocket microenvironment from external stimuli but also regulate the functions of neighboring cells including fibroblasts and inflammatory cells. To elucidate this mechanism, we characterized the effects of periodontopathic bacterium Eikenella corrodens 1073 components on the production of some inflammatory mediators in a human oral epithelial cell line (KB). In enzyme-linked immunosorbent assay (ELISA), the E. corrodens supernatant induced interleukin-6 (IL-6), IL-8 and prostaglandin E2 but not interferon-gamma (IFN-gamma) production by KB cells. After incubation with E. corrodens supernatant, KB cells showed a marked increase in the levels of IL-6, IL-8 and PG G/H synthase (cyclooxygenase)-2, but not IFN-gamma, gene expression by reverse-transcriptase polymerase chain reaction. All these E. corrodens products responsible for production of these inflammatory mediators resisted freezing and boiling and were present in a 10-kDa filtrate. These results imply that these soluble small-molecular-mass products from E. corrodens stimulate various inflammatory mediator productions by human oral epithelial cells and may play a role in the initiation of periodontal inflammation and subsequently perpetuate the inflammatory response during chronic infection.
(キーワード): Eikenella corrodens / 口腔上皮細胞 / プロスタグランディンE2 / インターロイキン6 / インターロイキン8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11555307; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034840691

(PubMed: 11555307, Elsevier: Scopus) Tadashi Nakanishi, Hirotoshi Shimizu, Yoshitaka Hosokawa and Takashi Matsuo :
An immunohistological study on cyclooxygenase-2 in human dental pulp.,
Journal of Endodontics, Vol.27, No.6, 385-388, 2001.

(要約): Characteristics of cyclooxygenase-2 (COX-2) expressing cells in human dental pulp were immunohistologically studied. Extirpated pulpal tissues from extracted teeth were examined to elucidate the localization and distribution of COX-2. Pulpal tissues were examined by the labeled streptavidin biotin method using specific mouse monoclonal antibodies for COX-2. Cell types of the COX-2 expressing cells were also investigated by the double stain technique using both monoclonal antibodies for CD68/macrophage and anti-COX-2. COX-2 expressing cells could be found in all of the inflamed pulps, and these cells were mostly distributed close to the area of accumulation of inflammatory cells. COX-2 was mainly expressed in fibroblasts rather than macrophages. In contrast, COX-2 expressing cells were scarcely found in the normal pulps. These findings indicate that pulpal fibroblasts, as well as macrophages, may participate in the production of prostaglandin through COX-2 expression in pulpal inflammation, and might be involved in the pathogenesis of irreversible pulpitis.
(キーワード): Adult / Antibodies, Monoclonal / Antigens, CD / Antigens, Differentiation, Myelomonocytic / Coloring Agents / Cyclooxygenase 2 / Dental Pulp / Fibroblasts / Fluorescent Dyes / Humans / Immunoenzyme Techniques / Immunohistochemistry / Isoenzymes / Macrophages / Membrane Proteins / Middle Aged / Peroxidases / Prostaglandin-Endoperoxide Synthases / Prostaglandins / Pulpitis
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00004770-200106000-00003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11487130; ● Search Scopus @ Elsevier (PMID): 11487130; ● Search Scopus @ Elsevier (DOI): 10.1097/00004770-200106000-00003

(DOI: 10.1097/00004770-200106000-00003, PubMed: 11487130) Akiko Kawasaki, Kunio ISHIKAWA, Toshiyuki Suge, Hirotoshi SHIMIZU, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU :
Effects of plaque control on the patency and occlusion of dentine tubules in situ.,
Journal of Oral Rehabilitation, Vol.28, No.5, 439-449, 2001.

(要約): To elucidate the nature of dentine hypersensitivity, the effects of plaque control on the patency and occlusion of dentinal tubules were investigated systematically in situ using human dentine slabs embedded in partial dentures. The dentine slabs were divided into three groups. In group I, the dentine slabs were kept in an oral cavity without plaque control. In group II, plaque was removed mechanically by brushing. Plaque control was carried out chemically using chlorhexidine in group III. After being kept in the oral cavities for 1, 2 and 3 weeks, the slabs were removed from the partial denture, followed by SEM observation to determine the morphological changes of the dentinal tubules. When no attempt was made to remove plaque, the diameter of tubule orifices increased to 390% of the original values within 3 weeks. In contrast, dentinal tubules were found to be occluded, i.e. the tubule orifices became <20% of the original value within 1 week when plaque control efforts were made, using either method of plaque control. We conclude that plaque control plays one of the key roles in the patency versus occlusion of dentinal tubules, and thus in the aetiology and natural reparative process of dentine hypersensitivity.
(キーワード): Adult / Anti-Infective Agents, Local / Calcium / Chlorhexidine / Dental Plaque / Dentin / Dentin Sensitivity / Dentin, Secondary / Electron Probe Microanalysis / Follow-Up Studies / Humans / Hydrogen-Ion Concentration / Magnesium / Male / Microscopy, Electron / Microscopy, Electron, Scanning / Phosphates / Phosphorus / Saliva / Spectrophotometry, Ultraviolet / Statistics as Topic / Toothbrushing
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11380784; ● Search Scopus @ Elsevier (PMID): 11380784

(PubMed: 11380784) Akihisa Urayama, Satoshi Nakabo, Takeshi Kimochi, Yoshihiro Nishitani, Junichi Doi, Takashi Matsuo and Masahiro Yoshiyama :
In Vitro Resin Bonding to Artificial Carious Dentin,
Journal of Hard Tissue Biology, Vol.10, No.1, 17-23, 2001.

(キーワード): carious dentin / dentin bonding / resin / artificial caries

Masahiro Yoshiyama, Akihisa Urayama, Takeshi Kimochi, Takashi Matsuo and DH Pashley :
Comparison of conventional vs self-etching adhesive bonds to caries-affected dentin,
Operative dentistry, Vol.25, No.3, 163-169, 2000. 藤中恵子, 川﨑有希子, 吉田佳子, 松岡希実, 尾崎和美, 中江英明, 松尾敬志 :
ヒト感染根管における侵入細菌の局在性に関する免疫組織学的研究,
日本歯科保存学雑誌, Vol.43, No.2, 407-416, 2000年.

(キーワード): 根尖性歯周炎 / 細菌 / 免疫染色

Kinya Murakami, Katsuhiko Hirota, Takashi Matsuo and Yoichiro Miyake :
Effect of antibiotics on CD15s-related antigen expressed on the Streptococcus anginosus group,
Dentistry in Japan, Vol.36, 22-27, 2000.

(キーワード): S. Anginosus Group / CD15s-Related Antigen / 抗生物質 (antibiotics)
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1570291224404669952

(CiNii: 1570291224404669952) 吉山昌宏, 伊東孝介, 西谷佳浩, 仲保聡, 土居潤一, 山田登美子, 鳥井康弘, 松尾敬志, 浦山明久, 木持健 :
根面う蝕へのシールド·レストレーションの応用:根面う蝕象牙質へのレジン接着性,
接着歯学, Vol.18, No.3, 226-231, 2000年.

(キーワード): レジン接着 / 根面う蝕 / シールド·レストレーション

Takeshi Kimochi, Masahiro Yoshiyama, Akihisa Urayama and Takashi Matsuo :
Adhesion of a new commercial self-etching/self-priming bonding resin to human caries-infected dentin,
Dental Materials Journal, Vol.18, No.4, 437-443, 1999.

(キーワード): Resin / Adhesion / caries-infected dentin

Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU :
Evaluation of post-treatment solutions for clinical use with the calsium phosphate precipitation method,
Journal of Dentistry, Vol.27, No.7, 487-496, 1999.

(要約): We have proposed a calcium phosphate precipitation (CPP) method that occludes dentine tubules with apatitic minerals for the treatment of dentine hypersensitivity. The current CPP method uses 1 mol/l NaOH as the post-treatment solution. However, its high pH is not desirable for clinical use. The aim of this study was to evaluate several solutions, especially buffer solutions, as post-treatment solutions for the CPP method. The CPP solution was mixed with several post-treatment solutions at various mixing ratios in test tubes. The precipitates were collected, and freeze-dried in a vacuum. Their weights were measured to evaluate the feasibility of buffer solution for the post-treatment solutions. Among the solutions, we selected one buffer solution and have done further evaluation using human dentine disks. The degree of occlusion of dentine tubules was evaluated by scanning electron microscopic observation and measurement of dentine permeability. Also, the composition of the precipitate was analyzed using energy-dispersive X-ray microanalysis and powder X-ray diffraction. Among the solutions, 1 mol/l NaHCO3 gave sufficient amounts of precipitate at a relatively mild pH, and thus was considered to be a good candidate for a post-treatment solution. Scanning electron microscopic observation showed that dentine tubules were occluded with the precipitate to a depth of approximately 10 microns from the surface when the CPP treatment was done twice, and dentine permeability was reduced to 98.8%. The precipitate was dicalcium phosphate dihydrate (DCPD; CaHPO4-2H2O) when the post-treatment solution was free of NaF. In contrast, the precipitate was an apatitic mineral with CaF2 as a by-product when the post-treatment solution contained NaF. We concluded that 1 mol/l NaHCO3 containing 0.3 mol/l NaF would be suitable as the post-treatment solution for the CPP method as it has a relatively mild pH and occludes dentinal tubules well.
(キーワード): Adult / Apatites / Buffers / Calcium Phosphates / Chemical Precipitation / Dentin / Dentin Permeability / Dentin Sensitivity / Electron Probe Microanalysis / Evaluation Studies as Topic / Feasibility Studies / Freeze Drying / Humans / Hydrogen-Ion Concentration / Microscopy, Electron, Scanning / Sodium Bicarbonate / Sodium Fluoride / Sodium Hydroxide / X線回折 (X-ray diffraction)
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0300-5712(99)00033-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10507204; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033191462

(DOI: 10.1016/S0300-5712(99)00033-0, PubMed: 10507204, Elsevier: Scopus) 綿城香, 石川利栄子, 蓮井義則, 上田(山口) 公子, 西野瑞穗, 尾崎和美, 松尾敬志 :
エナメル質形成不全症の1症例,
小児歯科学雑誌, Vol.37, No.3, 620-624, 1999年. 浦山明久, 吉山昌宏, 木持健, 松岡希実, 尾崎和美, 松尾敬志 :
人工う蝕象牙質へのレジン浸透性,
日本歯科保存学雑誌, Vol.42, No.3, 529-535, 1999年.

(キーワード): レジン / 浸透性 / 人工う蝕象牙質

Fumiko Michishige, Sumiko Yoshinaga, Eriko Harada, Katsuhiko Hirota, Yoichiro Miyake, Takashi Matsuo and Susumu Yasuoka :
Relationships between activity of daily living, and oral cavity care and the number of oral cavity microorganisms in patients with cerebrovascular diseases,
The Journal of Medical Investigation : JMI, Vol.46, No.1-2, 79-85, 1999.

(要約): We examined the relationships among the activity of daily living (ADL), oral cavity care, and the number of oral cavity microorganisms in 40 patients with cerebrovascular diseases (CVD). The CVD patients were classified into 4 groups, I, II, III and IV based on their ADL and the method used for oral cavity care. The ADL was highest in group I and lowest in group III. Only the patients of only group III could not eat by themselves and were receiving naso-esophageal feeding. Oral cavity care was performed by the patients themselves in groups I and IV, but was performed by caregivers in groups II and III. The group IV patients had no teeth, but could eat by themselves using full dentures. The numbers of microorganisms in the pharyngeal swabs from the 4 groups were measured and expressed as colony-forming units (cfu). The numbers of both Staphylococci spp. and Candida spp. were significantly higher in group III than in the other groups. Moreover, Pseudomonas aeruginosa was isolated only from patients of group III (in about 66%). The oral cavity care by caregivers was almost the same in groups II and III, but the numbers of oral cavity microorganisms were significantly higher in group III than in group II. These results indicated that microorganisms grow more easily in the oral cavities of CVD patients with low ADL compared with CVD patients with higher ADL, and that eating is thought to be important for the prevention of an increase of microorganisms in the oral cavity.
(キーワード): Oral cavity care / Aspiration Pneumonia / 脳血管障害 (cerebrovascular disease) / Pharyngeal Swab / 細菌 (bacteria)
(徳島大学機関リポジトリ): ● Metadata: 22626
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10408162; ● Search Scopus @ Elsevier (PMID): 10408162

(徳島大学機関リポジトリ: 22626, PubMed: 10408162) Hiromichi Yumoto, Hideaki Nakae, Keiko Fujinaka, Shigeyuki Ebisu and Takashi Matsuo :
Interleukin-6 (IL-6) and IL-8 are induced in human oral epithelial cells in response to exposure to periodontopathic Eikenella corrodens,
Infection and Immunity, Vol.67, No.1, 384-394, 1999. 吉山昌宏, 松尾敬志, 浦山明久, 木持健 :
う蝕感染象牙質へのレジン接着性:セルフェッチングボンディングシステムの効果,
接着歯学, Vol.17, No.3, 186-191, 1999年.

(キーワード): う蝕感染象牙質 / セルフェッチングボンディングシステム / レジン接着性 / マイクロテンザイル法 / シールド·レストレーション

吉山昌弘, 浦山明久, 木持健, 松岡希実, 尾崎和美, 松尾敬志 :
う蝕感染象牙質のレジン浸透性,
日本歯科保存学雑誌, Vol.42, No.1, 86-95, 1999年.

(キーワード): う蝕症 / レジン / 象牙質

Masahiro Yoshiyama, Takashi Matsuo, Shigeyuki Ebisu and David Pashley :
Regional bond strengths of self-etching/self-priming adhesive systems,
Journal of Dentistry, Vol.26, No.7, 609-616, 1998.

(要約): The purpose of this study was to measure the regional tensile bond strengths (TBS) at various portions of human tooth enamel and dentin, and to observe the resin-dentin interfaces of two commercially available self-etching/self-priming adhesive systems by scanning electron microscopy (SEM). Twelve extracted human cuspid teeth were used to measure TBS and four additional teeth were used for scanning electron microscopy (SEM). Outer enamel and dentin were removed from the labial tooth surfaces to form a long cavity preparation into middle dentin extending from the mid-crown to the apex of the root within the same tooth. Either Clearfil Liner Bond 2 (LB 2, Kuraray) or Fluoro Bond (FB, Shofu) was bonded to the surfaces, and covered with resin-composite. The resin-bonded teeth were serially sliced at right angles to the long axis of the tooth, and the bonded surfaces were trimmed to give a bonded cross-sectional surface area of 1 mm2 for TBS tests. LB 2 and FB showed significantly higher TBS in coronal, cervical and middle root dentin than that in enamel and apical root dentin. SEM showed that the thickness of the hybrid layer of both systems was about 1.0 microm in coronal, cervical and middle root dentin, and less than 0.5 microm in apical root dentin. These results suggested that the self-etching/self-priming systems produce good adhesion in coronal, cervical and middle root dentin by creating thin hybrid and transitional layers, but bonding to enamel and apical root dentin should be improved.
(キーワード): Adhesive resin / Dentin / Enamel bond strength
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0300-5712(97)00046-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9754751; ● Summary page in Scopus @ Elsevier: 2-s2.0-0032160584

(DOI: 10.1016/S0300-5712(97)00046-8, PubMed: 9754751, Elsevier: Scopus) Katsuhiko Hirota, Kinya Murakami, Ken Nemoto, Tsuneko Ono, Takashi Matsuo, Hiromi Kumon and Yoichiro Miyake :
Fosfomycin reduces CD15s-related antigen expression of Streptococcus pyogenes,
Antimicrobial Agents and Chemotherapy, Vol.42, No.5, 1083-1987, 1998.

(要約): We have previously shown the immunological mimicry of human sialyl-Lewis(x) (CD15s) by a surface antigen of Streptococcus pyogenes. This mimicking surface antigen may act as a ligand to the selectin family and may induce antibody production against CD15s on host cells, suggesting a possible role in the pathogenesis of S. pyogenes. In this study, the effects of antibiotics on the CD15s-related antigen expression of S. pyogenes were examined at a concentration below the MIC (sub-MIC). The amounts of CD15s on the surfaces of S. pyogenes cells and on the surfaces of S. pyogenes biofilms were determined by a whole-cell enzyme-linked immunosorbent assay and by laser scanning fluorescence microscopy, respectively, by using an anti-CD15s monoclonal antibody. At the sub-MICs, fosfomycin (1R,2S-1,2-epoxypropyl phosphonic acid), its enantiomer (1S,2R-1,2-epoxypropyl phosphonic acid), and benzylpenicillin significantly inhibited the CD15s expression of all strains studied. The effects of fosfomycin and its enantiomer on biofilms were also observed by scanning electron microscopy. Incubation of S. pyogenes with the sub-MIC of fosfomycin or its enantiomer, which has no antibacterial activity, reduced the amount of CD15s on the biofilm surface and made it smooth. These results suggest that fosfomycin or its enantiomer might be useful for preventing S. pyogenes adherence to human CD15s receptors and the resulting immunological pathogenicity.
(キーワード): Anti-Bacterial Agents / Antigens, Bacterial / Biofilms / Fosfomycin / Humans / Lewis X Antigen / Microbial Sensitivity Tests / Microscopy, Electron, Scanning / Molecular Mimicry / Streptococcus pyogenes
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AAC.42.5.1083
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9593131; ● Summary page in Scopus @ Elsevier: 2-s2.0-0031970340

(DOI: 10.1128/AAC.42.5.1083, PubMed: 9593131, Elsevier: Scopus) 吉山昌宏, 浦山明久, 篠原千尋, 湯本浩通, 松尾敬志 :
X線微小分析法による象牙質接着界面の解析,
日本歯科保存学雑誌, Vol.41, No.2, 461-466, 1998年.

(キーワード): 象牙質 / レジン / XMA

Yuichiro Noiri, Kazumi Ozaki, Hideaki Nakae, Takashi Matsuo and Shigeyuki Ebisu :
An immunohistochemical study on the localization of Porphyromonas gingivalis, Campylobacter rectus and Actinomyces viscosus in human periodontal pockets,
Journal of Periodontal Research, Vol.32, No.7, 598-607, 1997. 浦山明久, 塙隆夫, 中江英明, 松尾敬志, 淺岡憲三 :
チタンおよびチタン合金の口腔内バクテリア S.mutans に対する細菌学的性質,
日本金属学会誌, Vol.61, No.6, 471-474, 1997年.

(キーワード): titanium / titanium alloy / oral bacteria / bacterial adhesion / antibacterial property

Hiromichi Yumoto, Hiroyuki Azakami, Hideaki Nakae, Takashi Matsuo and Shigeyuki Ebisu :
Cloning, sequencing and expression of an Eikenella corrodens gene encoding a component protein of the lectin-like adhesin complex,
Gene, Vol.183, No.1-2, 115-121, 1996. Hiroyuki Azakami, Hiromichi Yumoto, Hideaki Nakae, Takashi Matsuo and Shigeyuki Ebisu :
Molecular analysis of the gene encoding a protein component of the Eikenella corrodens adhesin complex that is close to the carbohydrate recognition domain,
Gene, Vol.180, No.1-2, 207-212, 1996. Takashi Matsuo, Tadashi Nakanishi, Hirotoshi Shimizu and Shigeyuki Ebisu :
A clinical study of direct pulp capping applied to carious-exposed pulps,
Journal of Endodontics, Vol.22, No.10, 551-556, 1996. Takashi Matsuo, Chisato Tagawa, Kazumi Ozaki, Yuichiro Noiri and Shigeyuki Ebisu :
A Histological Analysis of T and B Cells in Periodontal Tissue Destruction,
Oral Medicine & Pathology, Vol.1, No.2, 77-83, 1996.

(要約): We immunohistologically examined the cell densities and distribution of T and B cells in periodontitis and analyzed their relationship in terms of periodontal tissue destruction. Specimens were labeled with monoclonal antibodies for T cells (CD3) and B cells (CD20) and developed using the double staining technique. CD3^+ cells appeared in low density infiltrates and increased gradually with the number of total infiltrates; in contrast, CD20^+ cells did not appear in small infiltrates but increased rapidly with the number of infiltrates. The number of fibroblastic cells negatively correlated with that of the total infiltrates and CD20^+ cells, but it did not correlate with the number of CD3^+ cells. These findings suggest that the T cells infiltrated into the inflamed sites at the onset and increased gradually with the development of inflammation and that B cells infiltrated later than T cells and their number increased rapidly with inflammation.
(キーワード): Immunohistology / T細胞 (T cell) / B cell / tissue destruction / 歯周炎 (periodontal disease)
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1572824502001680896

(CiNii: 1572824502001680896) 野杁由一郎, 尾崎和美, 藤中恵子, 岡本妙, 松尾敬志, 恵比須繁之 :
ヒト歯肉縁下プラークの研究ープラークフリーゾーンにおける歯周病関連細菌の走査免疫電顕法による検索ー,
日本歯周病学会会誌, Vol.38, No.1, 60-68, 1996年.

(キーワード): Plaque-free zone / Periodontal disease-associated bacteria / Scanning immunoelectron microscopy method

Takashi Matsuo, Tadashi Nakanishi and Shigeyuki Ebisu :
Immunoglobulins in periapical exudates of infected root canals: correlations with the clinical findings of the involved teeth,
Endodontics & dental traumatology, Vol.11, No.2, 95-99, 1995. Tadashi Nakanishi, Takashi Matsuo and Shigeyuki Ebisu :
Quantitative analysis of immunoglobulins and inflammatory factors in human pulpal blood from exposed pulps,
Journal of Endodontics, Vol.21, No.3, 131-136, 1995. 野杁由一郎, 藤中恵子, 近藤妙, 尾崎和美, 松尾敬志, 恵比須繁之 :
ヒト歯肉縁下プラークの研究 -プラーク細菌の定着·増殖における細菌バイオフィルムの関与についてー,
日本歯周病学会会誌, Vol.37, No.2, 329-336, 1995年.

(キーワード): Subgingival plaque / Bacterial biofilm / Scanning electron microscope

Takashi Matsuo, Shigeyuki Ebisu, Tadashi Nakanishi, Kouki Yonemura, Yasushi Harada and Hiroshi Okada :
Interleukin-1 alpha and interleukin-1 beta periapical exudates of infected root canals: correlations with the clinical findings of the involved teeth,
Journal of Endodontics, Vol.20, No.9, 432-435, 1994. Kazumi Ozaki, Takashi Matsuo, Hideaki Nakae, Yuichiro NOIRI, Masahiro YOSHIYAMA and Shigeyuki EBISU :
A quantitative comparison of selected bacteria in human carious dentine by microscopic counts.,
Caries Research, Vol.28, No.3, 137-145, 1994.

(キーワード): Actinomyces / Immunohistological stain / Microscopic counts / Mutans streptococci

Hideaki Nakae, Hiromichi Yumoto, Takashi Matsuo and Shigeyuki Ebisu :
Mitogenic stimulation of murine B lymphocytes by the N-acetyl-D-galactosamine specific bacterial lectin-like substance from Eikenella corrodens,
FEMS microbiology letters, Vol.116, No.3, 349-353, 1994. 野杁由一郎, 松尾敬志, 中江英明, 尾崎和美, 恵比須繁之 :
ヒト歯肉縁下プラークの研究 -ヒト歯周ポケットにおける歯周病原性細菌の局在性-,
日本歯周病学会会誌, Vol.36, No.2, 429-436, 1994年.

(キーワード): Periodontal poket / Periodontopathic bacteria / Localization / Immunohistochemical study

野杁由一郎, 松尾敬志, 中江英明, 尾崎和美, 恵比須繁之 :
ヒト歯肉縁下プラークの研究歯根付着性プラークの免疫組織学的検索,
日本歯周病学会会誌, Vol.36, No.1, 223-232, 1994年.

(キーワード): Tooth-attached subgingival plaque / Periodontopathic bacteria / Immunohistochemical stain

中西正, 松尾敬志, 多川知里, 白神俊之, 恵比須繁之 :
歯髄血液を用いた客観的歯髄診断法の開発に関する研究, --- 微量歯髄血液の採取法について ---,
日本歯科保存学雑誌, Vol.36, No.5, 1404-1409, 1993年.

(キーワード): 歯髄炎 / 診断 / 歯髄血液

尾崎和美, 松尾敬志, 中江英明, 野杁由一郎, 大木元玲子, 吉山昌宏, 恵比須繁之 :
歯根部の象牙質齲蝕病巣における侵入細菌の局在性に関する免疫組織学的研究,
日本歯科保存学雑誌, Vol.36, No.5, 1284-1291, 1993年.

(キーワード): 歯根部象牙質齲蝕 / 齲蝕関連細菌 / 光学顕微鏡的計測

尾崎和美, 松尾敬志, 中江英明, 野杁由一郎, 大木元玲子, 吉山昌宏, 恵比須繁之 :
小窩裂溝部および平滑面部の象牙質齲蝕病巣における侵入細菌の局在性に関する免疫組織学的研究,
日本歯科保存学雑誌, Vol.36, No.4, 1059-1069, 1993年.

(キーワード): 齲蝕関連細菌 / 酵素抗体法 / 光学顕微鏡的計測

P C Quan, S Watanabe, F Vuillier, R Pires, Takashi Matsuo, M Stanislawski, J Pillot and P J Bouvet :
Purification and partial amino acid sequence of suppressive lymphokine from a CD8+ CD57+ human T hybridoma,
Immunology, Vol.78, No.2, 205-209, 1993. Takashi Matsuo, Shigeyuki Ebisu, Yoshio Shimabukuro, Tsuyoshi Ohtake and Hiroshi Okada :
Quantitative analysis of immunocompetent cells in human periapical lesions: correlations with clinical findings of the involved teeth,
Journal of Endodontics, Vol.18, No.10, 497-500, 1992. 吉山昌宏, 尾崎和美, 野杁由一郎, 片岡健哉, 中西正, 大木元玲子, 中江英明, 松尾敬志, 恵比須繁之 :
象牙質知覚過敏症の治療法ー光重合型レジンライナーによる象牙細管封鎖効果ー,
日本歯科保存学雑誌, Vol.34, No.1, 76-81, 1991年.

(キーワード): 象牙質知覚過敏症 / 象牙細管封鎖 / 光重合型レジンランナー

北村正博, 恵比須繁之, 神山章, 松尾敬志, 三木靖夫, 岡田宏 :
歯肉溝浸出液中の炎症反応のメディエーターによる歯周病原性細菌の探索,
日本歯科保存学雑誌, Vol.34, No.2, 647-656, 1991年.

(キーワード): 炎症反応のメディエーター / 歯周病原性細菌 / 歯肉縁下プラーク細菌

今西和子, 松尾敬志, 中橋洋子, 鮫島義明, 恵比須繁之, 岡田宏 :
直接覆髄における術前および術中所見と術後成績の関係について,
日本歯科保存学雑誌, Vol.32, No.6, 1745-1750, 1989年.

(キーワード): 直接覆髄法 / 術後成績 / 診断

佐藤ますみ, 恵比須繁之, 中村慶子, 池辺ゆか, 原田泰, 松尾敬志, 岡田宏 :
根管治療の予後を左右する因子について, --- 特に過剰根管充填の場合 ---,
日本歯科保存学雑誌, Vol.32, No.3, 843-848, 1989年.

(キーワード): 過剰根管充填 / 感染根管治療 / 術後成績

島袋善夫, 大竹毅, 池辺ゆか, 松尾敬志, 恵比須繁之, 岡田宏 :
歯根肉芽腫と歯根嚢胞における免疫担当細胞の検索, --- T細胞サブセットと免疫グロブリン保有細胞との関係 ---,
日本歯科保存学雑誌, Vol.32, No.3, 713-719, 1989年.

(キーワード): 根尖病巣 / 免疫組織学的検索 / T細胞サブセット

米村幸城, 原田泰, 松尾敬志, 恵比寿繁之, 岡田宏 :
感染根管における根管浸出液中のインターロイキンー1(IL-1α/β)および免疫グロブリンと臨床所見との関連性,
日本歯科保存学雑誌, Vol.32, No.1, 168-177, 1989年.

(キーワード): 根管浸出液 / ILー1 / 免疫グロブリン

H Okada, Y Shimabukuro, Y Kassai, Hiro-O Ito, Takashi Matsuo, S Ebisu and Y Harada :
The Function of Gingival Lymphocytes on the Establishment of Human Periodontitis,
Advances in Dental Research, Vol.2, No.2, 364-367, 1988. Takashi Matsuo, Shinichiro Watanabe, Bouvet Jean-Pierre, Kolb Jean-Pierre and P Canh Quan :
Suppressor factor secreted by T hybridoma established from peripheral blood lymphocytes of a bone marrow transplantation patient. I. Establishment of human T-cell hybridoma and partial characterization of suppressor factor,
Cellular Immunology, Vol.116, No.2, 450-466, 1988.

(要約): A stable human T-cell hybridoma was established by cell fusion between activated human peripheral blood lymphocytes from an allogeneic bone marrow transplantation patient and the JD1-17 cell line, a subclone of the human T leukemia Jurkat cell line. This hybrid clone 1-8, which bore the surface phenotype of suppressor cells (CD8+HNK1+), spontaneously secreted a factor which, at high dilutions, suppressed the responses of T and B cells induced by mitogens and alloantigens. This suppressor factor was found to be heat-resistant (56 degrees C, 30 min), stable at alkaline but not acid pH, unaffected by 2-mercaptoethanol, and sensitive to trypsin. Preparative isoelectric focusing revealed an isoelectric point of 5.35. The suppressor activity was selectively absorbed by blast T cells. By gel filtration on Sephacryl S-200 and HPLC, the suppressor activity was found in two peaks corresponding to 40-45 kDa (monomer) and 90-95 kDa (dimer).
(キーワード): Absorption / Biological Factors / Bone Marrow Transplantation / Carbohydrates / Cell Cycle / Chromatography, Gel / Chromatography, High Pressure Liquid / Hot Temperature / Humans / Hybridomas / Hydrogen-Ion Concentration / Immune Tolerance / Interferons / Interleukin-2 / Interleukin-4 / Interleukins / Lymphocyte Activation / Phenotype / T-Lymphocytes, Regulatory
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/0008-8749(88)90244-4
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 2460252; ● Search Scopus @ Elsevier (PMID): 2460252; ● Search Scopus @ Elsevier (DOI): 10.1016/0008-8749(88)90244-4

(DOI: 10.1016/0008-8749(88)90244-4, PubMed: 2460252) 見田美千代, 恵比須繁之, 池田慶子, 大竹毅, 松澤裕之, 中山靖子, 松尾敬志, 岡田宏 :
感染根管の病原因子の臨床的評価について, --- 歯内治療の予後との関連性 ---,
日本歯科保存学雑誌, Vol.31, No.3, 795-802, 1988年.

(キーワード): 感染根管治療 / 術後成績 / 病原因子

北村正博, 神山章, 松尾敬志, 恵比須繁之, 岡田宏 :
歯周病変部と健常部における歯肉縁下プラーク細菌と歯肉溝浸出液中の炎症反応のメディエーター,
日本歯科保存学雑誌, Vol.35, No.2, 572-580, 1988年.

(キーワード): 歯肉溝浸出液 / 炎症反応のメディエーター / 歯肉縁下プラーク細菌

Hiro-O Ito, Yasushi Harada, Takashi Matsuo, Shigeyuki Ebisu and Hiroshi Okada :
Possible role of T cells in the establishment of IgG plasma cell-rich periodontal lesion--Augmentation of IgG synthesis in the polyclonal B cell activation response by autoreactive T cells,
Journal of Periodontal Research, Vol.23, No.1, 39-45, 1988. 野首孝祠, 長島正, 松矢篤三, 森田学, 岩倉政城, 福島正義, 荒木孝二, 前田芳信, 皆木省吾, 小川哲次, 松尾敬志, 松家洋子, 原宜興, 鳥居光男, 河野正司, 大山喬史 :
国立大学歯学部附属病院卒後臨床研修共通カリキュラムに基づいた研修項目の集計ならびに評価システムの開発,
日本歯科医学教育学会雑誌, Vol.17, No.1, 151-161, 1988年. 松尾敬志 :
Wistar-Furth 系ラット自然発生大腸癌の免疫学的研究:腫瘍抗原の解析と抗腫瘍抗体の検索,
大阪大学歯学雑誌, Vol.29, No.1, 86-100, 1984年.

(キーワード): Animals / Antibodies, Neoplasm / Antibody Formation / Antigens, Neoplasm / Carcinoembryonic Antigen / Carcinoma / Colonic Neoplasms / Immunity, Cellular / Rats / Rats, Inbred Strains
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 6590824; ● Search Scopus @ Elsevier (PMID): 6590824

(PubMed: 6590824) 松尾敬志, 岡田宏, 宮本誠, 谷洋一, 渡辺信一郎 :
自然発生大腸癌宿主Wistar-Furth 系ラット血清に認められた抗腫瘍抗体,
医学のあゆみ, Vol.128, No.8, 505-506, 1984年. 渡辺信一郎, 伯田宏, 松尾敬志, 原寛, 原志兎太郎 :
電子灸の施灸後の免疫機能への影響(1),
全日本鍼灸学会雑誌, Vol.31, No.1, 42-50, 1981年.

MISC

松尾敬志, 中西正, 湯本浩通, 平尾功治, 篠原千尋, Toshihiko Tominaga :
感染根管治療の考え方,
Journal of Oral Health and Biosciences, Vol.28, No.2, 87-92, 2016年.

(要約): It is a well-established fact that bacterial infection of the dental pulp ultimately results in the formation of apical periodontitis. And apical periodontitis may develop periapical leisons consisting granulomas and cysts. In this article, we show the pathologic condition of infected root canal systems and periapical lesions immunohistologically. We also describe the possible causes of refractory apical periodontitis that conventional root canal therapy could not work effectively. Finally,we explain our newly developing treatment for refractory apical periodontitis named electro-magnetic apical treatment, and illustrate clinical efficacy of this treatment.
(キーワード): infected root canal treatment / refractory apical periodontitis / pathological condition
(徳島大学機関リポジトリ): ● Metadata: 109873
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050845762395157248

(徳島大学機関リポジトリ: 109873, CiNii: 1050845762395157248) Toshiyuki Suge and Takashi Matsuo :
Effects of ammonium hexafluorosilicate concentration on crystallinity of hydroxyapatite powder and enamel,
Key Engineering Materials, Vol.529-530, 526-530, 2013.

(出版サイトへのリンク): ● Publication site (DOI): 10.4028/www.scientific.net/KEM.529-530.526
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84871313515

(DOI: 10.4028/www.scientific.net/KEM.529-530.526, Elsevier: Scopus) 弘田克彦, 湯本浩通, 星野由美, 村上圭史, 宮本幸治, 松尾敬志, 三宅洋一郎 :
水溶性MPCポリマーの口腔細菌付着抑制効果,
Bacterial Adherence & Biofilm, Vol.24, 47-49, 2011年.

(要約): 口腔細菌によるbiofilm，すなわちdental plaqueの形成にはいくつかのkey stepがあり，dental plaque形成の抑制のためにはそれらのkey stepに効果的に働く方法を選択する必要が有る．我々は，超親水性表面を得ることができる2-methacryloloxethyl phosphorylcholine(MPC)co-polymerを用いてプラスチック表面をコートした界面は微生物の付着抑制に関与することを報告してきた．本実験では5%水溶性MPCポリマーで処理したHydroxyapatite(HY)プレートと培養歯肉上皮細胞(RT-7)へのStreptococcus mutansの付着状況を顕微鏡下にて観察した．その結果，5%水溶性MPCポリマー処理でHYプレート及び培養歯肉上皮細胞上へのS.mutansの付着は抑制されることが示された．以上よりHYプレートや培養歯肉上皮細胞に吸着した水溶性MPCポリマーは，プラスチックへのコーティングと同様にS.mutansの付着抑制効果を発揮することが示唆された．

石川清美, 渡邊久恵, 三木千津, 久保万友美, 山下美由紀, 大森幸子, 石川利栄子, 荒木哲也, 蓮井義則, 湯本浩通, 松尾敬志 :
歯周病患者に対する禁煙指導について, --- ニコチネルの応用 ---,
歯科衛生士, Vol.26, No.9, 77-81, 2002年. 道重文子, 表崎沙矢, 金子有花, 坪内裕子, 前中康次, 村上綾梨, 山本晃代, 吉永純子, 松尾敬志, 安岡劭 :
口腔内細菌数と自覚感による口腔ケアの至適時間の検討,
徳島大学医療技術短期大学部紀要, Vol.10, 113-121, 2000年.

(要約): The sbjects were classified 3 groups. Oral care was effectively carried out at 10o'clok in the morning group, 15o'clok in the midday group and 19o'clok in the night group. The number of Streptococci spp. and Staphlococci spp. in the samples was quantitatively measured. These results showed that effect of mouth care is not influenced by the time when it is done, as far as it is effectively done once in a day, and suggested that in the subjects who can not perform oral care by themselves, and can not receive oral care service several times in a day. It is important to perform effective mouth care which remove completely dental plaque, once in a day, by caregivers.
(キーワード): 口腔ケア

三木千津, 石川清美, 渡邊久恵, 市原真由美, 久保万友美, 北林裕枝, 岡村由華, 細道美喜子, 石川利栄子, 綿城香, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
臼歯部の動揺を伴う歯周病患者への対応, --- 特にメインテナンスにおける歯科衛生士の役割 ---,
歯科衛生士, Vol.24, No.11, 81-85, 2000年. 石川清美, 渡邊久恵, 三木千津, 市原真由美, 久保万友美, 北林裕枝, 岡村由華, 細道美喜子, 石川利栄子, 綿城香, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
更年期障害を有する歯周病患者の一症例,
歯科衛生士, Vol.24, No.2, 81-84, 2000年. 渡邊久恵, 三好千奈, 三木千津, 石川清美, 池内明子, 蓮井義則, 石川利栄子, 綿城香, 石丸毅, 湯本浩通, 松尾敬志 :
口腔がん患者における歯周治療および管理の重要性,
歯科衛生士, Vol.23, No.9, 65-69, 1999年. 多田智代, 三好千奈, 渡邊久恵, 石川清美, 池内明子, 塚原ひとみ, 石川利栄子, 三森康智, 綿城香, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
喫煙習慣を持つ歯周病患者への対応,
歯科衛生士, Vol.22, No.8, 67-71, 1998年. 道重文子, 吉永純子, 寺尾紀子, 原田江梨子, 安岡劭, 弘田克彦, 三宅洋一郎, 柏原稔也, 市川哲雄, 松尾敬志 :
含嗽の咽頭ぬぐい液細菌数に対する影響,
徳島大学医療技術短期大学部紀要, Vol.7, 35-42, 1997年.

(キーワード): 嚥下性肺炎 / 含嗽 / 咽頭ぬぐい液細菌数 / レンサ球菌

小田原千奈, 多田智代, 渡邊久恵, 石川清美, 池内明子, 塚原ひとみ, 石川利栄子, 三森康智, 蓮井義則, 糸田川徳則, 松尾敬志 :
歯科衛生士の立場から見た若年性歯周炎,
歯科衛生士, Vol.21, No.8, 67-71, 1997年. 細木眞紀, 松尾敬志, 永田俊彦, 市川哲雄, 松香芳三, 田中栄二 :
徳島大学病院,
歯科審美, Vol.25, No.2, 196, 2013年. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Biological Roles of Extracellular DNA in Bacterial Infection,
Asean Plus and Tokushima Joint International Conference, Program and Proceeding Book, 47-48, 2012. Katsuhiko Hirota, Takeyoshi Yoneyama, Mayumi Sakamoto, Hiroshi Miyamoto, Masanori Kurihara, Shizuo Kayama, Keiji Murakami, Hiromichi Yumoto, Takashi Matsuo and Yoichiro Miyake :
High prevalence of Pseudomonas aeruginosa from oropharyngeal biofilm in patients with cerebrovascular infarction and dysphagia.,
Chest, Vol.138, No.1, 237-238, 2010.

(キーワード): Aged / Biofilms / Cerebral Infarction / Deglutition Disorders / 女性 (female) / Humans / 男性 (male) / Oropharynx / Pneumonia, Aspiration / Prevalence / Pseudomonas Infections / 緑膿菌 (Pseudomonas aeruginosa)
(出版サイトへのリンク): ● Publication site (DOI): 10.1378/chest.10-0240
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20605833; ● Search Scopus @ Elsevier (PMID): 20605833; ● Search Scopus @ Elsevier (DOI): 10.1378/chest.10-0240

(DOI: 10.1378/chest.10-0240, PubMed: 20605833)

総説・解説

湯本浩通, 平尾功治, 富永敏彦, 松尾敬志 :
高周波電流の根尖性歯周炎への応用,
歯内療法学会雑誌, Vol.41, No.2, 82-90, 2020年5月.

(徳島大学機関リポジトリ): ● Metadata: 115856

(徳島大学機関リポジトリ: 115856) 松尾敬志, 中西正 :
深在性う蝕への対応と歯髄の免疫応答,
日本歯科保存学雑誌, Vol.62, No.1, 1-7, 2019年2月.

(キーワード): 深在性う蝕 / 歯髄炎 (pulpitis) / 免疫応答
(出版サイトへのリンク): ● Publication site (DOI): 10.11471/shikahozon.62.1
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.11471/shikahozon.62.1

(DOI: 10.11471/shikahozon.62.1) Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Daisuke Takegawa, Tadashi Nakanishi and Takashi Matsuo :
The Roles of Odontoblasts in Dental Pulp Innate Immunity,
Japanese Dental Science Review, Vol.54, No.3, 105-117, Aug. 2018.

(要約): Odontoblasts located in the outermost layer of dental pulp form a natural barrier between mineralized tissues, dentin, and soft tissues, dental pulp, of the vital tooth, and they first recognize caries-related pathogens and sense external irritations. Therefore, odontoblasts possess a specialized innate immune system to fight oral pathogens invading into dentin. Generally, the rapid initial sensing of microbial pathogens, especially pathogen-associated molecular patterns (PAMPs) shared by microorganisms, are mediated by pattern recognition receptors (PRRs), such as Toll-like receptor and the nucleotide-binding oligomerization domain (NOD). The innate immune responses in odontoblasts initiated by sensing oral pathogens provide host protective events, such as inflammatory reactions, to produce a variety of pro-inflammatory mediators, including chemokines and cytokines. These attract various inflammatory cells and cause antibacterial reactions, such as the production of defensins, to kill microorganisms in the proximal region of the odontoblast layer. This review focuses on innate immunity, especially cellular and molecular mechanisms regarding the sensing of PAMPs from oral pathogens by PRRs, in odontoblasts and provides information for future studies for the development of novel therapeutic strategies, including diagnosis and treatment, to prevent exceeding dental pulp inflammation and preserve the dental pulp tissues.
(徳島大学機関リポジトリ): ● Metadata: 112960
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jdsr.2018.03.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30128058; ● Summary page in Scopus @ Elsevier: 2-s2.0-85044379499

(徳島大学機関リポジトリ: 112960, DOI: 10.1016/j.jdsr.2018.03.001, PubMed: 30128058, Elsevier: Scopus) 中西正, 松尾敬志 :
感染根管における細菌感染の実態,
日本歯科評論, Vol.77, No.11, 45-58, 2017年11月. 松尾敬志, 中西正 :
歯髄炎の病態形成における細菌侵襲と歯髄の反応性,
日本歯科評論, Vol.73, No.6, 9-11, 2013年6月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1524232504494800512

(CiNii: 1524232504494800512) 松尾敬志, 尾崎和美, 中西正, 湯本浩通, 高橋加奈子, 平尾功治, 武川大輔 :
歯髄疾患の病態と診断,
日本歯内療法学会雑誌, Vol.34, No.2, 67-76, 2013年5月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680166022016

(CiNii: 1390282680166022016) 松尾敬志, 中西正 :
8 薬物による歯痛制御,
ザ·クインテッセンス, Vol.22, No.6, 147-161, 2003年6月.

(キーワード): 歯痛 / 薬剤 / 象牙質知覚過敏 / 歯髄炎

藤中恵子, 篠原千尋, 渋谷拓也, 松尾敬志 :
難治性根尖性歯周炎走査型電子顕微鏡による観察を中心に,
日本歯科評論, Vol.63, No.3, 61-66, 2003年. 細川義隆, 中西正, 松尾敬志 :
歯周炎とケモカイン,
臨床免疫, Vol.38, No.2, 151-156, 2002年2月. 中西正, 高橋加奈子, 細川義隆, 尾崎和美, 松尾敬志 :
歯髄炎の病態形成における細菌侵襲と歯髄反応,
四国歯学会雑誌, Vol.14, No.2, 255-263, 2002年.

(キーワード): 歯髄炎 / う蝕関連細菌 / 免疫担当細胞 / プロスタグランディン / サイトカイン (cytokine)

松尾敬志 :
感染症としての齲蝕新しい発想の治療を目指して,
CLinic Todar, 2-10, 2001年11月. 湯本浩通, 松尾敬志, 市川哲雄 :
義歯とカンジダ,
医薬ジャーナル, Vol.37, No.10, 211-217, 2001年10月. Takashi Matsuo, Kazumi Ozaki, Hiromichi Yumoto and Masahiro Yoshiyama :
Caries-A disease of bacteria infection,
Caries Preventive restoration, 1-8, 2001. 中江英明, 湯本浩通, 山田真義, 篠原千尋, 松尾敬志 :
歯周炎発症における歯肉上皮細胞の役割,
四国歯学会雑誌, Vol.13, No.2, 259-265, 2001年.

(キーワード): 歯周病関連細菌 / 歯肉上皮細胞 / ケモカイン / 接着分子

吉山昌宏, 木持健, 浦山明久, 松尾敬志 :
楔状欠損·根面齲蝕修復の新しい流れ,
Vol.25, No.7, 165-168, 2000年6月. 吉山昌宏, 木持健, 浦山明久, 松尾敬志 :
接着性レジンを知覚過敏の臨床に生かす,
ザ·クインテッセンス, Vol.19, No.5, 63-69, 2000年5月.

(キーワード): 接着性レジン / 象牙質知覚過敏症 / 歯髄象牙質複合体

松尾敬志, 中江英明 :
歯科領域での抗菌剤の投与,
医薬ジャーナル, Vol.35, No.7, 131-138, 1999年7月. 吉山昌宏, 松尾敬志, 尾崎和美 :
齲蝕象牙質へのシールド·レストレーションの可能性細菌を封じ込める治療とその現在,
ザ·クインテッセンス, Vol.18, No.1, 77-89, 1999年1月.

(キーワード): 象牙質齲蝕 / シールド·レストレーション / 接着性レジン

松尾敬志, 中西正, 藤中恵子 :
難治性根尖性歯周炎病理と病態,
現代の根管治療の診断科学, 91-97, 1999年. 松尾敬志 :
齲蝕露髄歯における覆髄の適否とその診断指標,
四国歯学会雑誌, Vol.10, No.2, 223-230, 1998年.

(キーワード): 齲蝕露髄 / 直接覆髄 / 診断指標 / 歯髄血 / 炎症メディエータ

吉山昌宏, 松尾敬志 :
象牙質による接着の違い,
歯界展望別冊/わかる·できる接着, 42-45, 1997年11月. 吉山昌宏, 松尾敬志 :
楔状欠損部への接着,
ザ·クインテッセンス, Vol.16, No.1, 6-19, 1997年.

(キーワード): 象牙質 / 楔状欠損 / 接着

松尾敬志, 恵比須繁之 :
保存領域(歯髄炎·根尖病変)の免疫,
歯科ジャーナル, Vol.31, No.6, 889-897, 1990年6月.

(キーワード): 歯周疾患 / 免疫学的病因論 / 自己反応性T細胞 / 多クローン性B細胞活性化 / CD4/CD8比

松尾敬志 :
歯周疾患の免疫学的病因論,
歯科ジャーナル, Vol.28, No.5, 505-512, 1988年11月.

(キーワード): 歯周疾患 / 免疫学的病因論 / 自己反応性T細胞 / 多クローン性B細胞活性化 / CD4/CD8比

松尾敬志 :
もう一度，齲蝕を考える象牙質齲蝕を中心に,
日本歯科保存学雑誌, Vol.48, No.3, 345-350, 1988年6月. 渡辺信一郎, 松尾敬志, 相良憲次, 福原毅 :
Carcinoembryonic Antigen(CEA)の最近の進歩とその限界性,
日本臨牀, Vol.38, No.12, 87(4567)-94(4574), 1980年12月.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520854805328712576

(CiNii: 1520854805328712576)

講演・発表

Hiromichi Yumoto, Kouji Hirao, Yuki Hosokawa, Hitomi Kuramoto, Katsuhiko Hirota, Yoichiro Miyake and Takashi Matsuo :
Streptococcal histone-like DNA binding protein induces MINCLE expression on monocytes,
95th General Session & Exhibition of the International Association for Dental Research, San Francisco, Mar. 2017. Yuki Hosokawa, Hiromichi Yumoto, Kouji Hirao, Hitomi Kuramoto, Tadashi Nakanishi, Daisuke Takegawa and Takashi Matsuo :
Anti-inflammatory effects of polyphenols on rat odontoblastic cells,
95th General Session & Exhibition of the International Association for Dental Research (San Francisco), Mar. 2017. Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa and Takashi Matsuo :
Interferon-gamma modulates the innate immune response in odontoblast-like cells,
95th General session and exhibition of the IADR, Mar. 2017. Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Hiromichi Yumoto, Yuki Hosokawa and Takashi Matsuo :
Effect of interleukin-17A on CCL20 production from odontoblast-like cells,
95th General session and exhibition of the IADR, Mar. 2017. Susilowati Heni, Keiji Murakami, Takashi Amoh, Katsuhiko Hirota, Yoichiro Miyake, Hiromichi Yumoto and Takashi Matsuo :
Royal Jelly inhibits Pseudomonas aeruginosa adherence and reduces excessive inflammatory resoponses in human epithelial cells,
2016 IADR, Seoul, Jun. 2016. Toshihiko Tominaga, Kazuki Takahira, Hiromichi Yumoto, Takashi Matsuo, Naoki Bando and Kouji Hirao :
EMAT (Electromagnetic Apical Treatment): Clinical Application of Pulsed Electric Current Energization for Root Canal Treatment,
2015 Annual Session of the American Association of Endodontists, Seattle (USA), May 2015. Tadashi Nakanishi, Daisuke Takegawa, Hiromichi Yumoto, Kouji Hirao and Takashi Matsuo :
Interleukin-17 Regulates Inflammatory Mediator Expression in IL-1beta-stimulated Dental Pulp Cells,
93th General Session & Exhibition of the International Association for Dental Research, Boston (USA), Mar. 2015. Shindo Satoru, Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki and Takashi Matsuo :
The effect of genipin on dendritic cells activation,
93rd General Session & Exhibition of the IADR, Boston, Mar. 2015. Ikuko Hosokawa, Yoshitaka Hosokawa, Shindo Satoru, Kazumi Ozaki and Takashi Matsuo :
Melatonin inhibits inflammatory mediators productions in human periodontal ligament cells,
93rd General Session & Exhibition of the IADR, Boston, Mar. 2015. Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki and Takashi Matsuo :
IL-4 modulates chemokine productions from IL-1-stimulated human periodontal ligament cells,
93rd General Session & Exhibition of the IADR, Boston, Mar. 2015. Kouji Hirao, Hiromichi Yumoto, Chihiro Shinohara, Hosokawa Yuki and Takashi Matsuo :
Functional roles of NOD1 in odontoblasts on pulp innate immunity,
The 62nd annual meeting of japanese association for dental research, 99, Dec. 2014. Hiromichi Yumoto, Takashi Matsuo, Katsuhiko Hirota, Kouji Hirao, Miyazaki Tsuyoshi, Keiji Murakami, Natsumi Fujiwara and Yoichiro Miyake :
Anti-inflammatory and protective effects of 2-methacryloyloxyethyl-phosphorylcholine-polymer on oral epithelial cells,
92th General Session & Exhibition of the International Association for Dental Research (Cape Town, South Africa), Jun. 2014. Satoru Shindo, Yoshitaka Hosokawa, Ikuko Hosokawa and Takashi Matsuo :
The effect of genipin on MMP-1 and MMP-3 expression in IL-1beta-stimulated human periodontal ligament cells,
The 15th Joint Scientific Meeting of JSCD-KACD, Gyeongju, Korea, Nov. 2013. Hiromichi Yumoto, Katsuhiko Hirota, Kouji Hirao, Keiji Murakami, Yoichiro Miyake and Takashi Matsuo :
Pathogenic roles of Streptococcal histone-like protein in microbial infection,
The 15th Joint Scientific Meeting of JSCD-KACD (Gyeongju, Korea), Nov. 2013. Toshihiko Tominaga, Naoki Bando, Hiromichi Yumoto and Takashi Matsuo :
Trial of Electro-Magnetic Wave Irradiation Therapy for Vertical Root Fracture as Tooth Conservative Therapy: a Case report,
The 9th World Endodontic Congress, Tokyo, Japan, May 2013. Naoki Bando, Toshihiko Tominaga, Hiromichi Yumoto and Takashi Matsuo :
Clinical Application of Electro-Magnetic Wave Irradiation to the Treatment for Periapical Periodontitis; Case Report,
The 9th World Endodontic Congress, Tokyo, Japan, May 2013. Tadashi Nakanishi, Daisuke Takegawa, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Prostaglandin F2a Regulates Cytokine Expression in Dental Pulp Cells,
91th General Session & Exhibition of the IADR, Seattle, USA, Mar. 2013. Daisuke Takegawa, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Interferon-g modulates the innate immune response of dental pulp cells,
91th General Session & Exhibition of the IADR, Seattle, USA, Mar. 2013. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Biological Roles of Extracellular DNA in Bacterial Infection,
Asean Plus and Tokushima Joint International Conference, Yogyakarta, Dec. 2012. Hiromichi Yumoto, Toshihiko Tominaga, Kouji Hirao, Naoki Bando, Takashi Sumitomo, Kanako Takahashi and Takashi Matsuo :
Signaling Pathways and Gene Expression Activated by Electro-Magnetic-Wave in Osteoblastic-Cells,
90th General Session and Exhibition of the IADR, Iguazu Falls, Brazil, Jun. 2012. Kouji Hirao, Hiromichi Yumoto, Katsuhiko Hirota, Dali Liu, Nur Asikin, Kanako Takahashi, Takashi Matsuo and Yoichiro Miyake :
Global Gene Analysis in Monocytes Stimulated with Streptococcal Histone-Like Protein,
90th General Session and Exhibition of the IADR, Iguazu Falls, Brazil, Jun. 2012. Toshihiko Tominaga, Hiromichi Yumoto and Takashi Matsuo :
Clinical Application of Electro-Magnetic Wave Irradiation for Infected Root Canal Treatment -EMAT (Electro-Magnetic Apical Treatment) -,
2012 Annual Session of the American Association of Endodontists, Boston, USA, Apr. 2012. Hiromichi Yumoto, Toshihiko Tominaga and Takashi Matsuo :
Electro-Magnetic Wave Irradiation Stimulates Osteoblasts to Enhance Cell Growth and Gene Expression of Growth Factors,
2012 Annual Session of the American Association of Endodontists, Boston, USA, Apr. 2012. Katsuhiko Hirota, Hiromichi Yumoto, Nur Asikin, Kouji Hirao, Kanako Takahashi, Liu Dali, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Gene Expression Profiling of Human Monocytic THP-1 Cells Stimulated With Extracellular Streptococcus intermedius Histone-Like DNA Binding Protein,
International Union of Microbiological Societies 2011 Congress, Sep. 2011. Takashi Sumitomo, Toshihiko Sumitomo, Naoki Bando, Saki Hirao, Shinya Kako, Hiromichi Yumoto and Takashi Matsuo :
A Case Report; Electro-Magnetic Apical Treatment,
The 9th KAE-JEA Joint Meeting, Jul. 2011. Yoichiro Miyake, Katsuhiko Hirota, Hiromichi Yumoto, N Yamamoto, K Miyamoto and Takashi Matsuo :
Inhibition of the adherence of oral bacterial by MPC-polymer,
EUROBIOFILMS 2011 -Second European Congress on Microbial Biofilms-Basic and Dlinical Aspedts-, Copenhagen, Jul. 2011. Tsuyoshi Fujita, Hiromichi Yumoto, Kazuhisa Ouhara, Hideki Shiba, Tsuyoshi Miyagawa, S Matsuda, Hiroyuki Kawaguchi, Takashi Matsuo and Hidemi Kurihara :
Irsogladine Maleate Regulates Gingival Epithelial Barrier Functions In Inflammatory Condition,
89th General Session & Exhibition of the International Association for Dental Research, Mar. 2011. Tadashi Nakanishi, Kouji Hirao, Takegawa Daisuke, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Catechins suppress cyclooxygenase-2 expression in human dental pulp cells,
88th General Session and Exhibition of the IADR, Jul. 2010. Toshihiko Tominaga, Hiromichi Yumoto and Takashi Matsuo :
Novel Therapeutic Approach of Electro-Magnetic Wave for Infected Root Canal,
88th General Session and Exhibition of the IADR, Jul. 2010. Takegawa Daisuke, Tadashi Nakanishi, Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi and Takashi Matsuo :
Interferon-gamma enhances toll-like receptor ligand-induced cytokine production in pulpal cells,
88th General Session and Exhibition of the IADR, Jul. 2010. Hiromichi Yumoto, T. TOMINAGA, Kouji Hirao, Tomoko Kimura and Takashi Matsuo :
Inactivation and Bactericidal Activity against Oral Bacteria of Electro-Magnetic Wave,
88th General Session and Exhibition of the IADR, Jul. 2010. Toshihiko Tominaga, Takashi Sumitomo, Hiromichi Yumoto and Takashi Matsuo :
The effects of Electro-Magnetic-Wave on bacterial viability and osteoblastic cell proliferation,
American Association of Endodontics, 2009 Annual Session, Apr. 2009. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Pro-inflammatory Roles of NOD2 in Human Gingival Fibroblasts,
The 3rd International Symposium on "Oral Sciences to Improve the Quality of Life", Tokushima, Sep. 2008. Hiromichi Yumoto, Liu Dali, Keiji Murakami, Katsuhiko Hirota, Hideaki Nagamune, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
Gene-regulatory and Physiological Functions of Histone-like Protein from Streptococci,
86th General Session and Exhibition of the International Association for Dental Research, Toronto (Canada), Jul. 2008. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Pro-inflammatory Roles of NOD2 in Human Gingival Fibroblasts,
86th General Session and Exhibition of the International Association for Dental Research, Toronto, Jul. 2008. Kayo Mukai, Tadashi Nakanishi, Hiromichi Yumoto, Kanako Takahashi, Kouji Hirao and Takashi Matsuo :
Effects of Catechins on Cytokine Production in Human Odontoblast-like Cells,
86th General Session and Exhibition of the International Association for Dental Research, Jul. 2008. Kouji Hirao, Hiromichi Yumoto, Tadashi Nakanishi, Kanako Takahashi, Kayo Mukai, Miho Yoneda and Takashi Matsuo :
Functional Analysis of Pattern Recognition Receptor Expression in Pulpal Cells,
86th General Session and Exhibition of the International Association for Dental Research, Jul. 2008. Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Th17 cytokines enhance CCL20 production by human gingival fibroblasts.,
86th General Session and Exhibition of the International Association for Dental Research, Toronto, Jun. 2008. Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
The essentiality and involvements of streptocuccus intermedius histone-like protein in bacterial viability and normal growth,
The 2nd International Symposium on "The Future Direction of Oral Sciences in the 21st Century", Tokushima, Dec. 2007. Tomoko Kimura, Kazumi Ozaki, Keiji Murakami, Kikumi Fujisaka and Takashi Matsuo :
The Analysis of role of Phosphoenolpyruvate-Dependent Sucrose Phosphotransferase System in pathogenicity of Streptococcus mutans.,
第1回国際シンポジウム・FDワークショップ, Awaji, Mar. 2007. Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi, Kayo Mukai and Takashi Matsuo :
Expression and Functional Analyses of Pattern recognition Receptor (PRR) in Cultured Human Dental Pulp Cells,
The 1st international symposium and workshop "The Future Direction of Oral Sciences in the 21st century", Mar. 2007. Kouji Hirao, Hiromichi Yumoto, Kanako Takahashi, Kayo Mukai and Takashi Matsuo :
Expression and Functional Analyses of Pattern recognition Receptor (PRR) in Cultured Human Dental Pulp Cells,
The 8th Joint-Scientific Meeting between KACD & JSCD, Busan(Korea), Nov. 2006. Kayo Mukai, Tadashi Nakanishi, Yoshitaka Hosokawa, Kanako Takahashi and Takashi Matsuo :
Inhibitory effects of catechins on inflammatory reactions in pulp fibroblasts,
84th General Session & Exhibition of the IADR, Brisbane, Jun. 2006. Miho Yoneda, Hideaki Nakae, Hiromichi Yumoto, Chihiro Shinohara and Takashi Matsuo :
Expression pattern of claudin family in human gingival tissues,
84th General Session and Exhibition of the International Association for Dental Research, Jun. 2006. Akiko Kawasaki, Toshiyuki Suge, Kunio ISHIKAWA and Takashi Matsuo :
Effect of ammonium hexafluorosilicate on acid resistance of enamel and dentin,
Sydney, May 2004. Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa and Takashi Matsuo :
Occlusion of dentinal tubules with ammonium hexafluorosilicate for treatment of dentin hypersensitivity,
7th World Biomaterials Congress, Sydney, May 2004. Natsuko Ida, Kazumi Ozaki, Shinji Suenobu, Kanako Takahashi, Daisuke Yamaguchi and Takashi Matsuo :
Opsonized Streptococcus mutans influences maturation of human dendritic cells,
The 82nd General Session of IADR Meeting, Hawaii, Mar. 2004. Kazumi Ozaki and Takashi Matsuo :
Expression of adrenomedullin and its receptors in inflamed gingival tissue,
The 82nd General Session of IADR Meeting, Hawaii, Mar. 2004. Tadashi Nakanishi, Tomohiko Adachi, Kanako Takahashi and Takashi Matsuo :
Expression of interferon-gamma -inducible protein 10/CXCL10 in inflamed dental pulp,
82th General Session of the IADR(INternational Association of Dental Research), Honolulu, Mar. 2004. Kanako Takahashi, Tadashi Nakanishi, Tomohiko Adachi, Natsuko Ida, Hideaki Nakae and Takashi Matsuo :
Effects of Streptococcus mutans on MIP-3 α production by macrophages,
82th General Session of the IADR(INternational Association of Dental Research), Honolulu, Mar. 2004. Natsuko Ida, Kazumi Ozaki, Shinji Suenobu, Kanako Takahashi, Daisuke Yamaguchi and Takashi Matsuo :
Opsonized Streptococcus mutans influences maturation of human dendritic cells.,
Seoul, Nov. 2003. Takuya Shibutani, Kazumi Ozaki and Takashi Matsuo :
Comparison of root canal preparation by three Ni-Ti instruments,
Seoul, Nov. 2003. Daisuke Yamaguchi, Tadashi Nakanishi, Kanako Takahashi, Hideaki Nakae and Takashi Matsuo :
Expression of macrophage inflammatory protein-3alpha/CCL20 in human gingival epithelial cells,
81th General Session of the IADR(INternational Association of Dental Research), エルサレム, Jun. 2003. Kazumi Ozaki, Shinji Suenobu, Natsuko Ida and Takashi Matsuo :
Bacterial Viability Assay at an Electron Microscopic Leved,
The 81th General Session of IADR Meeting, Goteborg, Sweden, Jun. 2003. Shinji Suenobu, Kazumi Ozaki and Takashi Matsuo :
The Analysis of mRNA Expression of Glucosyltransferases and Dextranase A from Streptococcs mutans Adhered to the Dentin,
The 81th General Session of IADR Meeting, Goteborg, Sweden, Jun. 2003. Takuya Shibutani, Kazumi Ozaki and Takashi Matsuo :
Comparison of the Effects of Three Ni-Ti Preparation Techniques by Micro-computed Tomography,
The 81th General Session of IADR Meeting, Goteborg, Sweden, Jun. 2003. Akiko Kawasaki, Toshiyuki Suge, Kunio Ishikawa, Kazumi Ozaki, Takashi Matsuo and Shigeyuki Ebisu :
Ammonium hexafluorosilicate increase acid resistance of bovine enamel and dentin,
Journal of Dental Research, Vol.82, No.Special Issue B, B-71, Goteborg, Jun. 2003. Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Takashi Matsuo and Shigeyuki Ebisu :
Duraiton of ammonium hexafluorosilicate for dentinal tubules occlusion,
Journal of Dental Research, Vol.82, No.Special Issue B, B-47, Goteborg, Jun. 2003. Hiromichi Yumoto, Katsuhiko Hirota, Hideaki Nagamune, Kazuto Ohkura, Keiji Murakami, Masayoshi Yamada, Hideaki Nakae, Yoichiro Miyake and Takashi Matsuo :
Molecular analysis of histone-like protein from Streptococcus intermedius,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Masayoshi Yamada, Hiromichi Yumoto, Chihiro Shinohara, Hideaki Nakae, Shigeyuki Ebisu and Takashi Matsuo :
Eikenella corrodens Induces Endothelial Cells via MAPK Family pathways,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Yoshitaka Hosokawa, Tadashi Nakanishi, Daisuke Yamaguchi, Hiromichi Yumoto and Takashi Matsuo :
LARC-CCR6 Interactions play an important role in CD4+ T cell accumulation in periodontal diseased tissue,
80th General Session and Exhibition of the International Association for Dental Research, San Diego(USA), Mar. 2002. Tadashi Nakanishi, Kanako Takahashi, Yoshitaka Hosokawa, Daisuke Yamaguchi, Chihiro Shinohara, Hiromichi Yumoto and Takashi Matsuo :
Expression of macrophage inflammatory protein-3 α in human pulpitis,
80th General SEssion ofthe IADR(International Association of Dental Research), San Diego(USA), Mar. 2002. Akiko Kawasaki, Toshiyuki Suge, Kunio ISHIKAWA, Kazumi Ozaki and Takashi Matsuo :
Ammonium hexafluorosilicate increased acid resistance of bovine enamel and dentin,
Transactions of the fourth international congress on dental materials, Vol.16, 195, Honolulu, Jan. 2002. Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Takashi Matsuo and Shigeyuki EBISU :
Effects of ammonium hexafluorosilicate on dental tubules' occlusion,
Transactions of the fourth international congress on dental materials, Vol.16, 253, Honolulu, Jan. 2002. Takashi Matsuo, Keiko Fujinaka, Yoshiko Yoshida and Kazumi Ozaki :
Scanning electron microscope observations in the apices of roots with refractory apical periodontitis,
The 3rd Joint Scientific Meeting between KACD and JSCD, Seoul, Nov. 2001. Tadashi Nakanishi, Kanako Takahashi, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
An immunohistological study on the localization of selected bacteria and chemokines in human deep-carious teeth,
The International Conference on Dentin/Pulp complexs, Chiba, Jul. 2001. Akiko Kawasaki, Toshiyuki Suge, Kunio ISHIKAWA, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU :
Effects of ammonium hexafluorosilicate on occluion of dentinal tubules,
Journal of Dental Research, Vol.80, No.Special Issue, 750, Makuhari, Jun. 2001. Toshiyuki Suge, Kunio ISHIKAWA, Akiko Kawasaki, Kazuomi SUZUKI, Takashi Matsuo and Shigeyuki EBISU :
CaCl2 increase dentinal tubules occlusion ablity of potassium oxalate treatment,
Journal of Dental Research, Vol.80, No.Special Issue, 750, Makuhari, Jun. 2001. Hideaki Nakae, Masayoshi Yamada, Hiromichi Yumoto, Chihiro Shinohara and Takashi Matsuo :
Bacterial lectin of Eikenella corrodens induces cytokines and adhesion molecules,
Modern Periodontology New Directions in 21st Century-, Kanagawa(Japan), Jun. 2001. Hiromichi Yumoto, Daisuke Yamaguchi, Yoshitaka Hosokawa, Chihiro Shinohara, Tadashi Nakanishi, Hirotoshi Shimizu, Masayoshi Yamada, Hideaki Nakae and Takashi Matsuo :
Expression of IL-18 and IL-18 receptor in human periodontally diseased tissue,
Modern Periodontology New Directions in 21st Century-, Kanagawa(Japan), Jun. 2001. Chihiro Shinohara, Hiromichi Yumoto, Masayoshi Yamada, Hideaki Nakae, Shigeyuki Ebisu and Takashi Matsuo :
Secreted Products from Eikenella corrodens Activate Endothelial cells.,
The 86th Annual Meeting of the American Academy of Periodontology inconjunction with the Japanese Society of Periodontology, Hawaii(USA), Sep. 2000. Masayoshi Yamada, Hiromichi Yumoto, Hideaki Nakae, Chihiro Shinohara, Ebisu Shigeyuki and Takashi Matsuo :
Activation of mitogen-activated protein kinases in oral epithelial cells by Secreted Eikenella corrodens,
The 86th Annual Meeting of the American Academy of Periodontology inconjunction with the Japanese Society of Periodontology, Hawaii(USA), Sep. 2000. Hiromichi Yumoto, Hideaki Nakae, Masayoshi Yamada, Shigeyuki Ebisu and Takashi Matsuo :
Eikenella corrodens induces prostaglandin E2 production and cyclooxygenase-2 expression in oral epithelial cells,
11th International Conference on Periodontal Research, Goteborg(Sweden), Jun. 1999. Hideaki Nakae, Masayoshi Yamada, Hiromichi Yumoto, Shigeyuki Ebisu and Takashi Matsuo :
Infection of human oral epithelial cells with Eikenella corrodens upregulates ICAM-1 expression,
11th International Conference on Periodontal Research, Goteborg(Sweden), Jun. 1999. Kazumi Ozaki and Takashi Matsuo :
A three-dimensional examination of morphological changes in experimental cementum caries lesions,
77th General Session of IADR Meeting, Vancouver, Mar. 1999. Akiko Kawasaki, ISHIKAWA Kunio, Toshiyuki Suge, SUZUKI Kazuomi, Takashi Matsuo and EBISU Shigeyuki :
In vivo evaluation of plaque control on dentin hypersensitivity,
Journal of Dental Research, Vol.77, No.Special Issue, 571, Nice, Jun. 1998. Toshiyuki Suge, ISHIKAWA Kunio, Akiko Kawasaki, SUZUKI Kazuomi, Takashi Matsuo and EBISU Shigeyuki :
Comparison of dentinal tubules occlusion-CPP, Resin and Potassium Oxalate,
Journal of Dental Research, Vol.77, No.Special Issue, 651, Nice, Jun. 1998. Masahiro Yoshiyama and Takashi Matsuo :
Adhesion to Wedge-shaped Defects and Treatments of Dentin Hypersensitivity,
Modern Trends in Adhesive Dentistry Proceedings of the Adhesive Dentistry Forum `98 in Sapporo, 28-37, Sapporo, Feb. 1998. Akihisa Urayama, Takao Hanawa, Kenzo Asaoka and Takashi Matsuo :
Bacterial adhesion to saliva-coated titanium surface,
Transactions of the 3rd International Congress on Dental Materials, 244, Honolulu, Nov. 1997. Chihiro Shinohara, Kikuji Yamashita, Seiichiro Kitamura and Takashi Matsuo :
Effect of Acetazolamide on Osteoclasts and Osteoblasts in the rat,
The International Association for Dental Research, Orlando(USA), Mar. 1997. Shigeyuki Ebisu, T Yasunaga, Toshiyuki Suge, Akiko Kawasaki, Kunio Ishikawa, Kenzo Asaoka and Takashi Matsuo :
Acid resistance of bovine enamel after calcium phosphate precipitation method using dogs,
Journal of Dental Research, Vol.76, No.Special Issue, 180, Orlando, Mar. 1997. Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu :
Effects of plaque control on the occlusion of dentin tubules,
Journal of Dental Research, Vol.76, No.Special Issue, 180, Orlando, Mar. 1997. Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu :
Acid resistance of bovine enamel after calcium phosphate precipitation method,
Journal of Dental Research, Vol.76, No.Special Issue, 97, Orlando, Mar. 1997. Kazumi Ozaki, Yuichirou Noiri, Hideaki Nakae, Takashi Matsuo and Shigeyuki Ebisu :
A histopathological and microbiological study on experimental root-surface caries in humans,
74th General Session of IADR Meeting, San Francisco, Mar. 1996. Yuichirou Noiri, Kazumi Ozaki, Tae Okamoto, Keiko Fujinaka, Takashi Matsuo and Shigeyuki Ebisu :
Localization of periodontal disease-associated bacteria in human periodontal pockets,
74th General Session of IADR Meeting, San Francisco, Mar. 1996. H Okada, Y Shimabukuro, Y Kassai, Hiro-O Ito, Takashi Matsuo, S Ebisu and Y Harada :
The Function of Gingival Lymphocytes on the Establishment of Human Periodontitis,
Advances in Dental Research, Vol.2, No.2, 364-367, Kobe, Nov. 1988. 武川大輔, 中西正, 平尾功治, 湯本浩通, 細川由樹, 蔵本瞳, 松尾敬志 :
NODリガンド刺激したヒト象牙芽細胞様細胞におけるインターフェロンγの影響,
第150回日本歯科保存学会 2019年度春季学術大会, 2019年6月. 蔵本瞳, 湯本浩通, 平尾功治, 細川由樹, 松尾敬志 :
Caffeic Acid Phenetyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生誘導機序の解析,
第40回日本歯内療法学会学術大会 (東京), 2019年6月. 細川育子, 細川義隆, 尾崎和美, 松尾敬志 :
Carnosic acidはヒト口腔上皮細胞のIL-27誘導CXCR3リガンド産生を抑制する,
日本歯科保存学会2018年度秋季学術大会, 2018年11月. 平尾功治, 湯本浩通, 大塚邦紘, 石丸直澄, 松尾敬志 :
歯内由来病変と混同しやすい根尖病変様のエックス線透過像を有する症例,
第18回西日本歯内療法学会会研修会(2018年度), 2018年9月. 蔵本瞳, 湯本浩通, 平尾功治, 細川由樹, 中西正, 武川大輔, 松尾敬志 :
Caffeic Acid Phenetyl Ester (CAPE)の象牙芽細胞と骨芽細胞におけるVEGF産生誘導機序の解析,
第148回日本歯科保存学会春季学術大会, 2018年6月. 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
IL-27はヒト口腔上皮細胞のCXCR3リガンド産生を誘導する,
第61回日本歯周病学会春季学術大会, 2018年6月. 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
IL-29はTNF-αが誘導するヒト口腔上皮細胞のCXCL10産生を増強する,
第60回日本歯周病学会秋季学術大会, 2017年12月. 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
IL-29はヒト口腔上皮細胞のCXCL10産生を増強する,
第60回日本歯周病学会春季学術大会, 2017年10月. 平尾功治, 蔵本瞳, 湯本浩通, 細川由樹, 松尾敬志 :
プロポリス生理活性物質(CAPE; Caffeic Acid Phenetyl Ester)を用いた新規覆髄材の開発,
第17回西日本歯内療法学会研修会, 2017年9月. 平尾功治, 湯本浩通, 細川由樹, 蔵本瞳, 松尾敬志 :
ヒト歯髄組織ならびにラット象牙芽細胞(KN-3)におけるMincle発現と象牙芽細胞での機能解析,
第38回日本歯内療法学会学術大会 & 第14回日韓合同歯内療法学会学術大会, 2017年7月. 蔵本瞳, 湯本浩通, 平尾功治, 細川由樹, 中西正, 武川大輔, 松尾敬志 :
Caffeic Acid Phenethyl Ester (CAPE)のラット象牙芽細胞(KN-3)におけるVEGF産生に与える影響,
日本歯科保存学会2017年度春季学術大会(第146回), 2017年6月. 進藤智, 池田淳史, 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
シトルリン化ビメンチンは破骨細胞分化を促進する,
日本歯科保存学会2016年度秋季学術大会, 2016年10月. 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
Gomisin Nはヒト歯根膜由来細胞の炎症性サイトカイン産生を抑制する,
日本歯科保存学会2016年度秋季学術大会, 2016年10月. 湯本浩通, 平尾功治, 細川由樹, 蔵本瞳, 松尾敬志 :
40年以上前に外傷を受けた根未完成歯の根尖部に硬組織形成の誘導を試みた症例,
第16回西日本歯内療法学会研修会, 2016年9月. 細川由樹, 湯本浩通, 平尾功治, 松尾敬志 :
ラット象牙芽細胞様細胞(KN-3)に対するポリフェノールの炎症抑制効果,
第37回日本歯内療法学会学術大会, 2016年7月. 平尾功治, 湯本浩通, 細川由樹, 松尾敬志 :
Streptococci由来Histone-Like DNA binding protein (HLP)刺激の単球様細胞(THP-1)におけるMincle発現への影響,
日本歯科保存学会 2016年度春季学術大会(第144回), 2016年6月. 湯本浩通, 平尾功治, 細川由樹, 松尾敬志 :
Streptococci由来Histone-Like DNA binding proteinの分泌機構と病原因子としての呼吸器感染症における役割,
日本歯科保存学会 2016年度春季学術大会(第144回), 2016年6月. 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
Alkanninはヒト歯根膜由来細胞のIL-6およびCCL20産生を抑制する,
第59回春季日本歯周病学会学術大会, 2016年5月. 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
Alkanninはヒト歯根膜由来細胞のCCR5 ligand産生を誘導する,
日本歯科保存学会2015年度秋季学術大会, 2015年11月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 細川由樹, 松尾敬志 :
Interleukin-17がラット象牙芽細胞様細胞(KN-3)のCCL20産生に及ぼす影響,
日本歯科保存学会 2015年度秋季学術大会(第143回), 2015年11月. 細川由樹, 湯本浩通, 平尾功治, 中西正, 武川大輔, 松尾敬志 :
象牙芽細胞が有するMincleを介した視細胞認識機構とその歯髄炎症反応における役割,
日本歯科保存学会 2015年度秋季学術大会(第143回), 2015年11月. 湯本浩通, 平尾功治, 富永敏彦, 坂東直樹, 細川由樹, 松尾敬志 :
外科的歯内療法の依頼を受けた根側病変に対して高周波・電磁波照射を応用した症例,
第15回日本歯内療法学会西日本支部会研修会, 2015年9月. 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
IL-4がヒト歯根膜由来細胞のCCL11およびCCL20産生に及ぼす影響,
第58回秋季日本歯周病学会学術大会, 2015年9月. 富永敏彦, 高比良一輝, 北池光希, 坂東直樹, 湯本浩通, 平尾功治, 松尾敬志 :
EMAT (Electro-Magnetic Apical Treatment)∼根尖性歯周炎への応用に関する検討,
第36回日本歯内療法学会学術大会 & 第13回日韓合同歯内療法学会学術大会, 2015年7月. 平尾功治, 湯本浩通, 細川由樹, 中西正, 武川大輔, 松尾敬志 :
ラット象牙芽細胞(KN-3)の自然免疫反応におけるシグナル伝達経路の解析,
日本歯科保存学会2015年度春季学術大会(第142回), 2015年6月. 細川由樹, 湯本浩通, 平尾功治, 中西正, 武川大輔, 松尾敬志 :
ラット象牙芽細胞(KN-3)に対するカテキンとカフェイン酸の抗炎症作用,
日本歯科保存学会2015年度春季学術大会(第142回), 2015年6月. 松尾敬志, 中西正, 湯本浩通, 篠原千尋, 平尾功治 :
感染根管治療の考え方,
四国歯学会(第46回例会), 2015年3月. 進藤智, 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
Shikoninがヒト歯根膜由来細胞のIL-6およびIL-8産生に与える影響,
第141回日本歯科保存学会秋季学術大会, 2014年10月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 松尾敬志 :
Interleukin-17がヒト歯髄線維芽細胞の炎症メディエーター発現に及ぼす影響,
日本歯科保存学会2014年度秋季学術大会(第141回), 2014年10月. 細川由樹, 湯本浩通, 平尾功治, 高橋加奈子, 松尾敬志 :
イグサ抽出液のオーラルヘルスケア製品への応用に関する研究,
日本歯科保存学会2014年度秋季学術大会(第141回), 2014年10月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 天羽崇, 松尾敬志, 三宅洋一郎 :
Streptococcus intermedius Histone-Like DNA Binding Proteinの結合解析とExtracellular Vesicleとの関連,
第67回日本細菌学会中国・四国支部総会, 2014年10月. 太良尾浩生, 湯本浩通, 平尾功治, 富永敏彦, 坂東直樹, 高比良一輝, 榎本崇宏, 芥川正武, 池原敏孝, 松尾敬志, 木内陽介 :
電磁波照射による骨芽細胞培養培地内の電流密度分布とVEGF産生との関連,
第37回日本生体医工学会中国四国支部大会, 2014年10月. 湯本浩通, 平尾功治, 富永敏彦, 坂東直樹, 高比良一輝, 太良尾浩生, 榎本崇宏, 芥川正武, 池原敏孝, 松尾敬志, 木内陽介 :
電磁波照射による骨芽細胞の活性化と成長因子の発現・産生誘導,
第37回生体医工学会中国四国支部大会, 2014年10月. 石川久子, 永井浩美, 吉岡昌美, 伊賀弘起, 東雅之, 松尾敬志, 丹黒章 :
外来化学療法患者の口腔健康管理に対する意識調査,
第11回日本口腔ケア学会・旭川, 2014年6月. 永井浩美, 吉岡昌美, 石川久子, 東雅之, 河野文昭, 松尾敬志 :
地域歯科診療所における医科歯科連携の実態調査ー病院・施設への訪問診療の現状分析ー,
第11回日本口腔ケア学会, 2014年6月. 青田桂子, 山村佳子, 可児耕一, 高野栄之, 桃田幸弘, 松本文博, 河野文昭, 松尾敬志, 東雅之 :
徳島大学病院における周術期口腔機能管理の現状と課題,
第11回日本口腔ケア学会総会・学術大会, 2014年6月. 進藤智, 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
GenipinはE.coli LPSが誘導するヒト歯根膜細胞のIL-6およびIL-8産生を抑制する,
第140回日本歯科保存学会春季学術大会, 2014年6月. 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
活性型ビタミンDはIL-1β刺激ヒト歯根膜細胞の炎症性サイトカイン産生を抑制する,
第140回日本歯科保存学会春季学術大会, 2014年6月. 平尾功治, 湯本浩通, 中西正, 篠原千尋, 高橋加奈子, 武川大輔, 細川由樹, 松尾敬志 :
ラット象牙芽細胞様細胞(KN3)における細菌関連病原因子刺激に対する自然免疫反応の解析,
日本歯科保存学会2014年度春季学術大会(第140回), 2014年6月. 北中祐太郎, 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
genipinはTNF-αが誘導するヒト歯根膜由来細胞のIL-6産生を抑制する,
第57回春季歯周病学会学術大会, 2014年5月. 木村智子, 尾崎和美, 湯本浩通, 村上圭史, 菅原千恵子, 篠原千尋, 武川恵美, 三宅洋一郎, 松尾敬志, 河野文昭 :
Streptococcus mutans の病原性における scrA 遺伝子の役割,
第6回日本総合歯科学会総会・学術大会, 38, 2013年11月. 進藤智, 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
GenipinはIL-1betaが誘導するヒト歯根膜細胞のCCL20およびIL-6 産生を抑制する,
第139回日本歯科保存学会秋季学術大会, 2013年10月. 湯本浩通, 平尾功治, 松尾敬志 :
イグサ抽出液のう蝕および歯周病予防効果の評価,
第139回日本歯科保存学会秋季学術大会, 2013年10月. 進藤智, 細川義隆, 細川育子, 尾崎和美, 松尾敬志 :
GenipinはIL-1betaが誘導するヒト歯根膜細胞のCCL20およびIL-6産生を抑制する,
日本歯科保存学雑誌, 70, 2013年10月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Effect of extracellular DNA on pyocyanin production from Pseudomonas aeruginosa,
第27回Bacterial Agherence & Biofilm学術集会, Jul. 2013. 湯本浩通, 富永敏彦, 坂東直樹, 平尾功治, 松尾敬志 :
高周波・電磁波照射の歯内療法への応用ー基礎実験∼臨床症例ー,
第13回日本歯内療法学会西日本支部会研修会, 2013年7月. 弘田克彦, 湯本浩通, 村上圭史, 平尾功治, 高橋加奈子, 松尾敬志, 三宅洋一郎 :
Streptococcus intermediusヒストン様タンパク質のPAMPsとしての解析,
第22回Lancefieldレンサ球菌研究会&第45回レンサ球菌感染症研究会, 2013年6月. 富永敏彦, 湯本浩通, 坂東直樹, 平尾功治, 平尾早希, 加古晋也, 前田やよい, 山口由美子, 高比良一輝, 松尾敬志 :
Electro-Magnetic Apical Treatment ー嚢胞性疾患に対する対応ー,
第138回日本歯科保存学会春季学術大会, 2013年6月. 細川義隆, 細川育子, 進藤智, 尾崎和美, 松尾敬志 :
IL-6はIL-1βが誘導するヒト歯根膜由来細胞のCCL20産生を増強する,
日本歯周病学会会誌, 97, 2013年5月. 菅俊行, 野口直人, 松尾敬志, 吉本勝彦, 市川哲雄 :
日本大学松戸歯学部臨床実習視察報告,
第42回四国歯学会例会, 2013年3月. 大石慶二, 竹内久裕, 松尾敬志, 吉本勝彦, 市川哲雄 :
広島大学臨床実習視察報告,
第42回四国歯学会例会, 2013年3月. 細川育子, 細川義隆, 尾崎和美, 松尾敬志 :
Adrenomedullinが樹状細胞のTh17関連サイトカイン産生に関与するcell signalingに及ぼす影響,
日本歯科保存学雑誌, 2012年11月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
IL-17AはTNF-aが誘導するヒト歯肉線維芽細胞のCCL20産生を増強する,
日本歯科保存学雑誌, 2012年11月. 湯本浩通, 富永敏彦, 平尾功治, 松尾敬志 :
電磁波照射による骨芽細胞の活性化とGrowth Factorの発現誘導,
第10回日本再生歯科医学会学術大会, 2012年9月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Role of Extracellular DNA and DNA-binding protein in biofilm formation of Streptococcus intermeius,
第26回Bacterial Adherence & Biofilm学術集会, Jul. 2012. 細川義隆, 細川育子, 尾崎和美, 中西正, 中江英明, 松尾敬志 :
TNF-alphaとIL-4刺激が誘導するヒト歯肉線維芽細胞のCCL11産生に及ぼす緑茶カテキンの影響,
日本歯科保存学雑誌, 2012年6月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 高橋加奈子, 松尾敬志 :
prostaglandin F2aは歯髄細胞における炎症性メディエーター発現を調節する,
第136回日本歯科保存学会春季学術大会, 2012年6月. Nur Asikin, Katsuhiko Hirota, Hiromichi Yumoto, Kouji Hirao, Kanako Takahashi, Keiji Murakami, Takashi Matsuo and Yoichiro Miyake :
Roles of extracellular DNA and DNA-binding protein in cell aggregation, biofilm formation and antibiotic tolerance of Streptococcus intermedius,
第21回Lancefieldレンサ球菌研究会&第44回レンサ球菌感染症研究会合同開催, Jun. 2012. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
IL-22はIL-1betaが誘導するヒト歯肉線維芽細胞のCCL20産生を増強する,
日本歯周病学会会誌, 2012年5月. 細川義隆, 細川育子, 尾崎和美, 中西正, 松尾敬志 :
TWEAKがヒト歯肉線維芽細胞のIL-1beta誘導CCL20産生に与える影響,
日本歯科保存学雑誌, 2011年10月. 湯本浩通, 富永敏彦, 平尾功治, 高橋加奈子, 松尾敬志 :
高周波・電磁波照射による骨芽細胞のGrowth Factorの発現・産生誘導,
第135回日本歯科保存学会秋季学術大会, 2011年10月. 細川義隆, 細川育子, 尾崎和美, 中西正, 中江英明, 松尾敬志 :
TheaflavinがTNFSF14刺激ヒト歯肉線維芽細胞のIL-6産生に与える影響,
日本歯科保存学雑誌, 2011年6月. 武川大輔, 中西正, 平尾功治, 湯本浩通, 高橋加奈子, 松尾敬志 :
ヒト培養歯髄細胞の自然免疫応答に対するインターフェロンγの影響,
第134回日本歯科保存学会春季学術大会, 2011年6月. 湯本浩通, 富永敏彦, 柴田眞吾, 武川大輔, 高橋加奈子, 木村智子, 平尾功治, 松尾敬志 :
高周波・電磁波照射を歯内療法に応用した症例,
第134回日本歯科保存学会春季学術大会, 2011年6月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
TLR3 ligandがヒト歯肉線維芽細胞のIL-1beta誘導CCL20産生に与える影響,
日本歯周病学会会誌, 2011年5月. 進藤智, 細川義隆, 細川育子, 尾崎和美, 中西正, 中江英明, 松尾敬志 :
Oncostatin Mが誘導するヒト歯肉線維芽細胞のCXCL10産生に及ぼす紅茶ポリフェノールの影響,
日本歯周病学会会誌, 2011年5月. 近藤洋史, 小野恒子, 弘田克彦, 村上圭史, 三宅洋一郎, 湯本浩通, 高橋加奈子, 平尾功治, 松尾敬志 :
THP-1細胞のケモカイン産生に及ぼす口腔レンサ球菌ヒストン様DNA結合タンパク質の影響,
第34回徳島県医学検査学会, 2010年12月. 細川義隆, 細川育子, 尾崎和美, 中西正, 中江英明, 松尾敬志 :
CatechinがTNFSF14刺激ヒト歯肉線維芽細胞のIL-6産生に与える影響,
日本歯科保存学雑誌, 2010年10月. 湯本浩通, 高橋加奈子, 平尾功治, 松尾敬志 :
Streptococcus intermedius由来histon様DNA結合タンパク質がTHP-1細胞のサイトカインバランスに及ぼす影響,
第133回日本歯科保存学会秋季学術大会, 2010年10月. 中西正, 武川大輔, 平尾功治, 湯本浩通, 高橋加奈子, 松尾敬志 :
ヒト歯髄細胞におけるサイトカイン発現に対するProstaglandin F2alpha の影響,
日本歯科保存学会2010年度秋季学術大会(第133回), 2010年10月. 橋本玲奈, 細川義隆, 細川育子, 尾崎和美, 中西正, 中江英明, 松尾敬志 :
IL-1betaが誘導するヒト歯肉線維芽細胞のCXCL10産生に及ぼす緑茶カテキンの影響,
日本歯周病学会会誌, 2010年9月. 住友孝史, 富永敏彦, 坂東直樹, 平尾早希, 湯本浩通, 松尾敬志 :
Electro-Magnetic Apical Treatmentを行った1例,
第31回日本歯内療法学会学術大会, 2010年7月. 弘田克彦, 湯本浩通, 星野由美, 村上圭史, 宮本幸治, 松尾敬志, 三宅洋一郎 :
水溶性MPCポリマーの口腔細菌付着抑制効果,
Bacterial Adherence &Biofilm学術集会, 2010年7月. 坂東直樹, 富永敏彦, 住友孝史, 湯本浩通, 松尾敬志 :
難治性根尖性歯周炎に対する電磁波の応用-EMAT (Electro-Magnetic Apical Treatment)-,
第10回日本歯内療法学会西日本支部研修会, 2010年7月. 藤田剛, 應原一久, 湯本浩通, 林田浩一, 柴秀樹, 岩田倫幸, 内田雄士, 宮川剛史, 河口浩之, 松尾敬志, 栗原英見 :
イソグラジンマレイン酸による歯肉上皮細胞のバリアー機能制御について,
第132回日本歯科保存学会春季学術大会, 2010年6月. 藤田剛, 應原一久, 湯本浩通, 林田浩一, 永原隆吉, 宮川剛史, 岩田倫幸, 柴秀樹, 河口浩之, 松尾敬志, 栗原英見 :
Aggregatibacter actinomycetemcomitansが歯肉上皮細胞のclaudin-1発現および機能に及ぼす影響,
第53回春季日本歯周病学会学術大会, 2010年5月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
IL-17AはIL-1betaが誘導するヒト歯肉線維芽細胞のCCL20産生を増強する,
日本歯周病学会会誌, 2010年5月. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hideaki Nakae and Takashi Matsuo :
Adrenomedullin inhibits CXCL10 production by human gingival fibroblasts,
56th Annual Meeting of Japanese Association for Dental Research, Nov. 2008. 細川育子, 細川義隆, 尾崎和美, 中江英明, 松尾敬志 :
Toll-like Receptor Ligands刺激が誘導する単球のCCL20産生に及ぼすAdrenomedullinの影響,
第128回日本歯科保存学会秋季学術大会, 2008年11月. 細川育子, 細川義隆, 尾崎和美, 中江英明, 松尾敬志 :
ヒト歯肉線維芽細胞におけるTLR4 ligandが誘導するCXCL10産生に及ぼすAdrenomedullinの影響,
第51回日本歯周病学会秋季学術大会, 2008年10月. 細川育子, 細川義隆, 尾崎和美, 中江英明, 松尾敬志 :
Toll-like Receptor Ligands刺激が誘導する単球のIL-1beta産生に及ぼすAdrenomedullinの影響,
第128回日本歯科保存学会春季学術大会, 2008年6月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
Flagellinがヒト歯肉線維芽細胞のサイトカイン産生および接着分子発現に与える影響,
第128回日本歯科保存学会春季学術大会, 2008年6月. 平尾功治, 湯本浩通, 向井佳代, 松尾敬志 :
カテキンのヒト培養歯髄細胞に対する抗炎症効果,
第29回日本歯内療法学会, 2008年5月. 富永敏彦, 湯本浩通, 松尾敬志 :
Electro-Magnetic Apical Treatment,
第29回日本歯内療法学会, 2008年5月. 細川育子, 細川義隆, 尾崎和美, 中江英明, 松尾敬志 :
Adrenomedullinがヒト歯肉線維芽細胞のToll-like Receptor Ligands誘導サイトカイン産生に与える影響,
第51回日本歯周病学会春季学術大会, 2008年4月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
Toll-like receptor ligandsがヒト歯肉線維芽細胞のCXCL10産生に与える影響,
第51回日本歯周病学会春季学術大会, 2008年4月. 大石美佳, 松尾敬志, 野間隆文 :
歯学部学生委員会が取り組んだ高年次学生の合宿研修によるキャリア形成支援について,
2008年1月. Liu Dali, Hiromichi Yumoto, Keiji Murakami, Katsuhiko Hirota, Shizuo Kayama, Takashi Matsuo and Yoichiro Miyake :
The essentiality and involvements of Streptococcus intermedius histone-like protein in bacterial viability and normal growth,
The 2nd international symposium on "The future direction of oral sciences in the 21st century", Dec. 2007. 平尾功治, 湯本浩通, 向井佳代, 高橋加奈子, 中西正, 松尾敬志 :
カテキンは細菌やその構成成分に対する培養歯髄細胞の反応を調節する,
第127回日本歯科保存学会秋季学術大会, 2007年11月. 湯本浩通, 平尾功治, 高橋加奈子, 向井佳代, 中西正, 松尾敬志 :
培養歯髄細胞におけるPattern Recognition Receptor (PRR)を介した接着分子の発現,
第127回日本歯科保存学会秋季学術大会, 2007年11月. 細川育子, 細川義隆, 尾崎和美, 中江英明, 松尾敬志 :
Adrenomedulinがヒト歯肉線維芽細胞のサイトカイン産生に与える影響の解析,
第127回日本歯科保存学会秋季学術大会, 2007年11月. Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Hiromichi Yumoto, Hideaki Nakae and Takashi Matsuo :
Proinflammatory Roles of NOD2 in Human Gingival Fibroblasts,
第55回国際歯科研究学会日本部会(JADR)総会・学術大会, Nov. 2007. Dali LIU, 湯本浩通, 松尾敬志, 三宅洋一郎 :
Histone-like DNA binding protein of Streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via activation of ERK1/2 and JNK pathways.,
日本歯周病学会50周年記念大会, 2007年9月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
サイトカインがヒト歯肉線維芽細胞のCXCL10産生に与える影響,
日本歯周病学会50周年記念学術大会, 2007年9月. 篠原千尋, 山下菊治, 松尾敬志, 北村清一郎, 河野文昭 :
炭酸脱水酵素阻害剤アセタゾラミド(Az)が破骨細胞および骨組織に与える影響(優秀ポスター賞受賞),
第16回硬組織再生生物学会学術大会, 2007年9月. 木村智子, 尾崎和美, 藤坂菊美, 松尾敬志 :
Streptococcus mutans の病原性におけるスクロース輸送系遺伝子の役割について,
第126回日本歯科保存学会春季学術大会, 2007年6月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
ヒト歯肉線維芽細胞におけるCCL17産生の解析,
第50回春季日本歯周病学会学術大会, 2007年5月. 米田美穂, 中江英明, 湯本浩通, 篠原千尋, 松尾敬志 :
炎症性サイトカインがヒト歯肉上皮細胞間接着機構に及ぼす影響,
第50回日本歯周病学会春季学術大会, 2007年5月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
サイトカイン刺激によるヒト歯肉線維芽細胞におけるCXCL16産生の解析,
第36回日本免疫学会総会・学術集会, 2006年12月. 平尾功治, 湯本浩通, 高橋加奈子, 向井佳代, 松尾敬志 :
う蝕関連細菌が歯髄細胞における自然免疫応答に及ぼす影響,
第125回日本歯科保存学会秋季学術大会, 2006年11月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
サイトカインがヒト歯肉線維芽細胞上のICAM-1およびVCAM-1発現に与える影響,
第49回秋季日本歯周病学会学術大会, 2006年10月. 向井佳代, 中西正, 細川義隆, 高橋加奈子, 松尾敬志 :
カテキンが歯髄由来線維芽細胞の炎症性サイトカインおよび接着分子の発現誘導に与える影響,
第27回日本歯内療法学会学術大会, 2006年7月. 藤坂菊美, 尾崎和美, 松尾敬志 :
S. mutans com遺伝子の象牙質への侵入に及ぼす影響について,
第124回日本歯科保存学会春季学会, 2006年5月. 米田美穂, 中江英明, 湯本浩通, 篠原千尋, 松尾敬志 :
歯周病変局所における上皮細胞間接着機構の動態,
第124回日本歯科保存学会春季学会, 2006年5月. 向井佳代, 中西正, 細川義隆, 高橋加奈子, 足立智彦, 松尾敬志 :
カテキンが歯髄由来線維芽細胞の炎症性サイトカインおよび接着分子の発現誘導に与える影響,
第124回日本歯科保存学会春季学術大会, 2006年5月. 平尾功治, 湯本浩通, 高橋加奈子, 足立智彦, 向井佳代, 松尾敬志 :
培養歯髄細胞におけるPattern Recognition Receptor (PRR)の発現と機能解析,
第124回日本歯科保存学会春季学術大会, 2006年5月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
TWEAKがヒト歯肉線維芽細胞における炎症性サイトカイン産生ならびに接着分子発現に与える影響の解析,
第49回春季日本歯周病学会学術大会, 2006年4月. 菅悦子, 三木千津, 石川清美, 大森幸子, 蓮井義則, 尾崎和美, 松尾敬志 :
患者のコンプライアンスに配慮した歯周治療について,
第49回春季日本歯周病学会学術大会, 2006年4月. 足立智彦, 平尾功治, 湯本浩通, 高橋加奈子, 向井佳代, 中西正, 中江英明, 松尾敬志 :
歯髄組織および細菌刺激THP-1細胞におけるCXCR3 ligands(特にIP-10)の発現解析,
第123回秋季日本歯科保存会, 2005年11月. 細川義隆, 細川育子, 中江英明, 尾崎和美, 松尾敬志 :
炎症性サイトカインおよびLPSはヒト歯肉線維芽細胞のCCL20産生を誘導する,
第48回秋季日本歯周病学会学術大会, 2005年9月. 細川育子, 細川義隆, 尾崎和美, 松尾敬志 :
Langerhans細胞における抗菌ペプチドの発現について,
第48回秋季日本歯周病学会学術大会, 2005年9月. 大森幸子, 三木千津, 石川清美, 菅悦子, 蓮井義則, 尾崎和美, 松尾敬志 :
咬合に問題を有する歯周病患者の治療経過について,
第48回秋季日本歯周病学会学術大会, 2005年9月. 米田美穂, 中江英明, 篠原千尋, 松尾敬志 :
歯肉上皮細胞におけるClaudinの発現について,
第122回日本歯科保存学会春季学会, 2005年6月. 向井佳代, 中西正, 細川義隆, 高橋加奈子, 足立智彦, 松尾敬志 :
Toll-like receptor リガンドによる歯髄由来繊維芽細胞のIL-8, VEGF, 接着分子発現誘導,
第122回春季日本歯科保存学会, 2005年6月. 足立智彦, 中西正, 高橋加奈子, 松尾敬志 :
歯髄線維芽細胞における細菌由来因子によるIP-10発現,
第122回春季日本歯科保存学会, 2005年6月. 向井佳代, 中西正, 細川義隆, 高橋加奈子, 足立智彦, 松尾敬志 :
Toll-like receptor リガンドによる歯髄線維芽細胞のIL-8，VEGF，接着分子の発現誘導,
第122回日本歯科保存学会春季学術大会, 2005年6月. 菅悦子, 三木千津, 石川清美, 大森幸子, 蓮井義則, 尾崎和美, 松尾敬志 :
糖尿病を有する患者の歯周治療における管理の重要性,
第48回秋季日本歯周病学会学術大会, 2005年4月. 藤坂菊美, 末延慎司, 尾崎和美, 松尾敬志 :
培養条件変化によるStreptococcus mutansの動態に関する解析, --- 変異株を用いて培養した象牙質板の形態学的観察 ---,
第121回日本歯科保存学会秋季学会, 2004年11月. 末延慎司, 藤坂菊美, 尾崎和美, 松尾敬志 :
培養条件変化によるStreptococcus mutansの動態に関する解析, --- Glucosyltransferase関連遺伝子とグルカン合成量の関係 ---,
第121回日本歯科保存学会秋季学会, 2004年11月. 菅俊行, 川﨑有希子, 松尾敬志, 石川邦夫, 恵比須繁之 :
フッ化ジアミンシリケートの象牙細管封鎖効果の持続性およびアパタイト誘導能の評価,
日本歯科保存学雑誌, Vol.47, 100, 2004年11月. 三木千津, 石川清美, 菅悦子, 大森幸子, 蓮井義則, 尾崎和美, 松尾敬志 :
口腔癌患者における歯周治療および長期的管理の重要性,
第47回秋季日本歯周病学会学術大会, 2004年10月. 細川義隆, 細川育子, 尾崎和美, 中江英明, 松尾敬志 :
ヒト歯肉線維芽細胞におけるCXCL12産生ならびに機能的解析,
第47回秋季日本歯周病学会学術大会, 2004年10月. 菅悦子, 三木千津, 石川清美, 大森幸子, 蓮井義則, 尾崎和美, 松尾敬志 :
歯周治療中に発見した口腔粘膜疾患の一例,
第47回春季日本歯周病学会学術大会, 2004年5月. 足立智彦, 中西正, 高橋加奈子, 松尾敬志 :
歯髄炎の病態形成におけるIP-10の役割,
第33回日本免疫学会総会, 2003年12月. 高橋加奈子, 中西正, 足立智彦, 位田夏子, 中江英明, 松尾敬志 :
THP-1細胞からのサイトカイン産生におけるStrepptococcus mutansの影響について,
第33回日本免疫学会総会, 2003年12月. 足立智彦, 中西正, 高橋加奈子, 向井佳代, 松尾敬志 :
歯髄炎におけるCXCL10の発現に関する研究,
第119回秋季日本歯科保存学会, 2003年11月. 渋谷拓也, 尾崎和美, 松尾敬志, 石川邦夫, 恵比須繁之 :
マイクロCTによる3種Ni-Ti製ロータリーファイルの比較,
第119回日本歯科保存学会秋季大会, 2003年11月. 坂本治美, 尾崎和美, 大石慶二, 川﨑有希子, 横山希実, 松尾敬志 :
大規模ネットワークにおける歯周病検査・歯科保健指導の電子記録ならびにその運用について,
日本歯周病学会会誌, Vol.45, 197, 2003年10月. 大森幸子, 渡邊久恵, 三木千津, 石川清美, 山下美由紀, 蓮井義則, 尾崎和美, 松尾敬志 :
歯肉退縮にともなう発音及び審美障害に対する対応, --- ステントを用いた症例 ---,
第46回秋季日本歯周病学会学術大会, 2003年10月. 菅俊行, 川﨑有希子, 尾崎和美, 松尾敬志, 石川邦夫, 恵比須繁之 :
フッ化ジアミンシリケートによる歯質耐酸性の向上効果,
第118回日本歯科保存学会春季学会, 2003年6月. 位田夏子, 尾崎和美, 末延慎司, 高橋加奈子, 山口大輔, 松尾敬志 :
S.mutans刺激によるヒト樹状細胞の成熟化の異差について,
第118回日本歯科保存学会春季学会, 2003年6月. 渡邊久恵, 三木千津, 石川清美, 山下美由紀, 蓮井義則, 尾崎和美, 松尾敬志 :
歯周治療におけるリコールシステムの現状, --- アンケートによる患者の意識調査 ---,
第46回春季日本歯周病学会学術大会, 2003年4月. 高橋加奈子, 中西正, 山口大輔, 細川義隆, 湯本浩通, 尾崎和美, 中江英明, 松尾敬志 :
歯髄炎の病態形成におけるLARCおよびCCR6の役割,
第32回日本免疫学会, 2002年12月. 石川清美, 渡邊久恵, 三木千津, 久保万友美, 山下美由紀, 蓮井義則, 尾崎和美, 松尾敬志 :
小学校全児童を対象にした経年的な歯科検診・検査の解析, --- 歯科衛生士による口腔保健指導の効果について ---,
第45回秋季日本歯周病学会学術大会, Vol.44, 209, 2002年10月. 尾崎和美, 木持健, 末延慎司, 松尾敬志 :
象牙細管に侵入させた細菌の超微組織学的検索,
第116回日本歯科保存学会春季学会, Vol.45, 46, 2002年5月. 篠原千尋, 中西正, 末延慎司, 尾崎和美, 松尾敬志 :
抜歯に至った難治性根尖性歯周炎の1症例, --- マイクロCTならびに走査型電子顕微鏡による原因の考察 ---,
第116回日本歯科保存学会春季学会, Vol.45, 114, 2002年5月. 山口大輔, 細川義隆, 篠原千尋, 湯本浩通, 中西正, 中江英明, 松尾敬志 :
歯周炎病変局所におけるFractalkineおよびCX3CR1の発現,
第45回春季日本歯周病学会, 2002年4月. 山下美由紀, 渡邉久恵, 三木千津, 石川清美, 久保万友美, 蓮井義則, 湯本浩通, 松尾敬志 :
歯科衛生士の卒後研修の重要性とその意義,
第45回春季日本歯周病学会, 2002年4月. 山下美由紀, 渡邉久恵, 三木千津, 石川清美, 久保万友美, 蓮井義則, 湯本浩通, 松尾敬志 :
歯科衛生士の卒後研修の重要性とその意義,
香川県歯科医学大会, 2002年2月. 久保万友美, 山下美由紀, 渡邉久恵, 三木千津, 石川清美, 蓮井義則, 湯本浩通, 松尾敬志 :
在宅患者の口腔ケアにおける歯科衛生士の役割,
香川県歯科医学大会, 2002年2月. 細川義隆, 中西正, 山口大輔, 清水洋利, 尾崎和美, 中江英明, 松尾敬志 :
歯周炎病変局所におけるLARCおよびCCR6の発現,
第31回日本免疫学会, 2001年12月. 山口大輔, 細川義隆, 湯本浩通, 中西正, 中江英明, 松尾敬志 :
歯周炎病変局所におけるIL-18とその機能に関連する分子の発現について,
第31回日本免疫学会学術集会, 2001年12月. 末延慎司, 尾崎和美, 位田夏子, 川﨑有希子, 菅俊行, 松尾敬志 :
銀担持シリカを添加した合着用グラスアイオノマーセメントの諸性質,
第115回秋季日本歯科保存学会, 2001年11月. 高橋加奈子, 中西正, 細川義隆, 篠原千尋, 湯本浩通, 尾崎和美, 松尾敬志 :
炎症歯髄におけるLARCおよびCCR6の発現,
第115回秋季日本歯科保存学会, 2001年11月. 細川義隆, 中西正, 山口大輔, 清水洋利, 湯本浩通, 尾崎和美, 松尾敬志 :
歯周炎病変局所におけるLARCおよびCCR6の発現,
第44回秋季日本歯周病学会学術大会, 2001年10月. 湯本浩通, 弘田克彦, 山田真義, 中江英明, 村上圭史, 三宅洋一郎, 松尾敬志 :
Streptococcus intermedius histone-like proteinをコードする遺伝子のクローニングと発現,
第44回秋季日本歯周病学会学術大会, 2001年10月. 久保万友美, 渡邉久恵, 三木千津, 石川清美, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
在宅患者の口腔ケアにおける歯科衛生士の役割,
第44回秋季日本歯周病学会学術大会, 2001年10月. 山田眞義, 湯本浩通, 篠原千尋, 中江英明, 松尾敬志 :
歯周病関連細菌Eikenella corrodensが血管内皮細胞に及ぼす影響,
四国歯学会, 2001年6月. 藤中恵子, 川﨑有希子, 吉田佳子, 尾崎和美, 松尾敬志 :
難治性根尖性歯周における根尖部の走査型電子顕微鏡による検索,
第114回日本歯科保存学会, 2001年6月. 菅俊行, 川﨑有希子, 松尾敬志, 石川邦夫, 鈴木一臣, 恵比須繁之 :
フッ化ジアミンシリケートによる象牙質知覚過敏賞の治療法, --- 象牙細管封鎖効果の評価 ---,
日本歯科保存学雑誌, Vol.44, 114, 2001年6月. 松尾敬志, 中西正, 高橋加奈子, 尾崎和美, 中江英明 :
歯髄炎の不可逆性化に関する臨床的および病理学的考察,
第14回「歯内療法の集い」, 2001年5月. 三木千津, 渡邊久恵, 石川清美, 石川利栄子, 石丸毅, 蓮井義則, 湯本浩通, 松尾敬志 :
歯科衛生士の海外卒後研修に参加して -歯周組織・口腔粘膜疾患と国際協力についてー,
第44回春季日本歯周病学会学術大会, 2001年4月. 山口大輔, 細川義隆, 清水洋利, 篠原千尋, 湯本浩通, 中西正, 松尾敬志 :
歯周炎の病変局所におけるIL-18およびIL-18レセプターの発現,
第44回春季日本歯周病学会学術大会, 2001年4月. 中西正, 松岡希実, 高橋加奈子, 尾崎和美, 中江英明, 松尾敬志 :
深在う蝕における侵入細菌の局所と歯髄反応,
第113回秋季日本歯科保存学会, 2000年11月. 尾崎和美, 湯本浩通, 篠原千尋, 木持健, 松尾敬志 :
象牙質齲蝕に侵入した細菌種の同定, --- 電顕用樹脂包埋試料から抽出した16S rDNAを用いて ---,
第113回日本歯科保存学会秋季学会, 2000年11月. 細川義隆, 山口大輔, 清水洋利, 中西正, 尾崎和美, 中江英明, 松尾敬志 :
歯周炎の病巣局所におけるケモカインおよびケモカイン受容体の発現,
第30回日本免疫学会総会, 2000年11月. 湯本浩通, 篠原千尋, 山田真義, 中江英明, 恵比須繁之, 松尾敬志 :
Eikenella corrodens培養上清による血管内皮細胞の活性化機序の解析,
第43回秋季日本歯周病学会学術大会, 2000年10月. 石川清美, 渡邊久恵, 三木千津, 久保万友美, 石丸毅, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
歯周病患者に対する禁煙指導について -ニコチネルの応用―,
第43回秋季日本歯周病学会学術大会, 2000年10月. 山田眞義, 中江英明, 湯本浩通, 篠原千尋, 藤中恵子, 松尾敬志 :
上皮細胞のICAM-1発現誘導におけるEikenella corrodens付着因子の関与について,
第43回春季日本歯周病学会学術大会, 2000年5月. 渡邊久恵, 三木千津, 石川清美, 久保万友美, 石丸毅, 石川利栄子, 蓮井義則, 尾崎和美, 松尾敬志 :
口腔ケアで完治した難治性の口角ビランの一例,
第43回春季日本歯周病学会学術大会, 2000年5月. 久保万友美, 渡邊久恵, 三木千津, 石川清美, 石丸毅, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
瘢痕性類天疱瘡を有する患者の口腔管理,
第43回春季日本歯周病学会学術大会, 2000年5月. 菅俊行, 川﨑有希子, 松尾敬志, 石川邦夫, 鈴木一臣, 恵比須繁之 :
シュウ酸カリウム処理法への塩化カルシウム添加効果,
日本歯科保存学雑誌, Vol.43, 102, 2000年4月. 清水洋利, 細川義隆, 山口大輔, 中西正, 尾崎和美, 中江英明, 松尾敬志 :
歯周炎の病巣局所におけるリンパ球浸潤に対するケモカインの関与,
第29回日本免疫学会総会, 1999年12月. 藤中恵子, 吉田佳子, 松岡希実, 川﨑有希子, 菅俊行, 松尾敬志 :
難知性根尖性歯周炎における根尖部の細菌侵襲の実態,
日本歯科保存学雑誌, Vol.42, 57, 1999年11月. 三木千津, 石川清美, 渡邊久恵, 石丸毅, 石川利栄子, 蓮井義則, 湯本浩通, 松尾敬志 :
臼歯部の動揺を伴う歯周病患者への対応 -特にメインテナンスにおける衛生士の役割―,
第42回秋季日本歯周病学会学術大会, 1999年10月. 尾崎和美, 中西正, 藤中恵子, 松尾敬志 :
歯根部象牙質齲蝕誘発モデルの細菌学ならびに形態学的検索,
第110回日本歯科保存学会春季学会, 1999年5月. 浦山明久, 木持健, 松岡希実, 尾崎和美, 吉山昌宏, 松尾敬志 :
人工う蝕象牙質へのレジン浸透性, --- 培養期間が接着力に及ぼす影響 ---,
第110回日本歯科保存学会春季学会, 1999年5月. 木持健, 浦山明久, 尾崎和美, 吉山昌宏, 松尾敬志 :
齲蝕象牙質へのレジン浸透性, --- 試作カリエスコンディショナーの応用 ---,
第109回日本歯科保存学会春季学会, 1998年11月. 菅俊行, 石川邦夫, 川﨑有希子, 鈴木一臣, 松尾敬志, 恵比須繁之 :
アパタイト析出法による象牙質知覚過敏症の治療法,
四国歯学会雑誌, 1998年6月. 浦山明久, 尾崎和美, 吉山昌宏, 松尾敬志 :
人工齲蝕象牙質へのレジン浸透性,
第108回日本歯科保存学会春季学会, 1998年6月. 松岡希実, 吉田佳子, 川﨑有希子, 菅俊行, 野杁由一郎, 松尾敬志 :
ヒト感染根管の根尖部に認められた細菌の免疫組織学的解析,
日本歯科保存学雑誌, Vol.41, 71, 1998年6月. 尾崎和美, 藤中恵子, 糸田川徳則, 松尾敬志 :
歯根部齲蝕誘発モデルの細菌学ならびに形態学検索, --- 長期経過例について ---,
第107回日本歯科保存学会秋季学会, 1997年11月. 吉山昌宏, 糸田川徳則, 尾崎和美, 松尾敬志 :
齲蝕象牙質へのレジン浸透性, --- コラゲナーゼの応用 ---,
第107回日本歯科保存学会秋季学会, 1997年11月. 向井知里, 吉田佳子, 尾崎和美, 野杁由一郎, 松尾敬志 :
ヒト感染根管における細菌侵入に免疫組織学的解析,
第106回日本歯科保存学会春季学会, 1997年6月. 吉山昌宏, 尾崎和美, 松尾敬志 :
齲蝕象牙質へのレジン浸透性,
第106回日本歯科保存学会春季学会, 1997年6月. 川﨑有希子, 菅俊行, 松尾敬志, 石川邦夫, 恵比須繁之 :
アパタイト析出法がエナメル質の結晶性および耐酸性におよぼす影響について,
日本歯科保存学雑誌, Vol.39, 118, 1997年6月. Akiko Kawasaki, Kunio Ishikawa, Toshiyuki Suge, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu :
Effect of apatite forming method on the crystallinity of hydroxyapatite powder,
Transaction of the 12 th Symposium on apatite, 6-7, Dec. 1996. Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu :
Apatite forming method -duration of dentinal tubules occlusion-,
Transactions of the 12th Symposium on apatite, 4-5, Dec. 1996. Toshiyuki Suge, Kunio Ishikawa, Akiko Kawasaki, Kenzo Asaoka, Takashi Matsuo and Shigeyuki Ebisu :
A new treatment method for dentin hypersensitivity -apatite forming method-,
Transactions of the 12th Symposium on apatite, 2-3, Dec. 1996. 吉山昌宏, 篠原千尋, 湯本浩通, 松尾敬志 :
X線微小分析法による象牙質接着界面の解析,
第105回日本歯科保存学会秋季学会, 1996年11月. 中西正, 清水洋利, 尾崎和美, 中江英明, 松尾敬志 :
多形核白血球の集積化による歯髄組織破壊機構の解析, --- 歯髄細胞の剥離とフィブロネクチンの動態 ---,
第105回日本歯科保存学会春秋季学会, 1996年11月. 吉山昌宏, 篠原千尋, 松尾敬志, 稲井紀通, 佐野秀彦, 田上順次 :
セルフエッチングプライマーによる歯頸部摩耗象牙質の微細形態学的変化,
第104回日本歯科保存学会春季学会, 1996年5月.

(キーワード): 象牙質 / 接着性レジン

尾崎和美, 川戸純奈, 野杁由一郎, 松尾敬志, 恵比須繁之 :
歯根部齲蝕誘発モデルにおける齲蝕関連細菌の免疫電顕による検索,
第104回日本歯科保存学会春季学会, 1996年5月. 平尾清司, 中江英明, 尾崎和美, 松尾敬志, 恵比須繁之 :
歯周病原細菌の付着を促進させるヒト顎舌下腺唾液糖蛋白に対するモノクロナール抗体の作製,
第39回春季日本歯周病学会学術大会, 1996年4月. 平尾清司, 中江英明, 松尾敬志, 恵比須繁之 :
歯周病原細菌の付着を促進させるヒト顎下下腺唾液糖蛋白に対するモノクロナール抗体の作製,
第39回春季日本歯周病学会学術大会, 1996年4月. 尾崎和美, 川戸純奈, 野杁由一郎, 松尾敬志, 恵比須繁之 :
ヒト口腔内における歯根部齲蝕誘発モデルの細菌学ならびに形態学的検索,
第103回日本歯科保存学会秋季学会, 1995年10月. 野杁由一郎, 藤中恵子, 岡本妙, 尾崎和美, 松尾敬志, 恵比須繁之 :
ヒト歯周ポケット底部における歯周関連細菌の免疫電顕法による検索,
第38回秋季日本歯周病学会学術大会, 1995年10月. 白神俊之, 尾崎和美, 松尾敬志, 恵比須繁之 :
感染根管処置に反応せず，抜歯に至った1症例,
第2回「歯内療法の集い」, 1995年6月. 白神俊之, 松尾敬志, 尾崎和美, 野杁由一郎, 恵比須繁之 :
根尖病巣を有する歯の歯根部象牙細管侵入細菌の免疫組織学的検索,
第101回日本歯科保存学会秋季学会, 1994年11月. 野杁由一郎, 近藤妙, 尾崎和美, 中江英明, 松尾敬志, 恵比須繁之 :
ヒト歯周ポケットにおける異種菌体間相互作用の形態学的検索,
第37回日本歯周病学会秋季学会, 1994年10月. 野杁由一郎, 松尾敬志, 中江英明, 尾崎和美, 恵比須繁之 :
ヒト歯肉縁下プラークの研究, --- ヒト歯周ポケットにおける歯周病原性細菌の局在性 ---,
第17回四国歯学会, 1994年7月. 野杁由一郎, 近藤妙, 尾崎和美, 中江英明, 松尾敬志, 恵比須繁之 :
ヒト歯周ポケットにおける歯周病関連細菌の免疫組織学的2重染色法による検索,
第36回秋季日本歯周病学会学術大会, 1993年10月. 白神俊之, 松尾敬志, 尾崎和美, 野杁由一郎, 恵比須繁之 :
歯根部象牙細管に侵入した細菌の免疫組織学的検索,
第98回日本歯科保存学会春季学会, 1993年6月. 白神俊之, 松尾敬志, 松尾敬志, 野杁由一郎, 恵比須繁之 :
歯根部象牙細管に侵入した細菌の免疫組織学的検索,
第98回日本歯科保存学会春季学会, 1993年6月. 野杁由一郎, 尾崎和美, 中江英明, 松尾敬志, 恵比須繁之 :
ヒト歯周ポケットにおける歯周病関連細菌の局在性の検索,
第36回春季日本歯周病学会学術大会, 1993年5月. 尾崎和美, 松尾敬志, 中江英明, 吉山昌宏, 恵比須繁之 :
象牙質齲蝕病巣における侵入細菌の免疫組織学的検索,
第15回四国歯学会, 1992年6月. 尾崎和美, 松尾敬志, 中江英明, 吉山昌宏, 恵比須繁之 :
象牙質齲蝕における侵入細菌の免疫組織学的検索,
第96年日本歯科保存学会春季学会, 1992年6月. 尾崎和美, 松尾敬志, 中江英明, 吉山昌宏, 恵比須繁之 :
齲蝕における象牙質侵入細菌の動態に関する免疫組織化学的検討,
第95回日本歯科保存学会秋季学会, 1991年11月. 松尾敬志, 中西正, 片岡健哉, 尾崎和美, 野杁由一郎, 大木元玲子, 中江英明, 吉山昌宏, 恵比須繁之 :
覆髄法の適応範囲の拡大に関する臨床的検討,
第14回四国歯学会, 1991年7月. 吉山昌宏, 尾崎和美, 野杁由一郎, 片岡健哉, 中西正, 大木元玲子, 中江英明, 松尾敬志, 恵比須繁之 :
象牙質知覚過敏症の治療法, --- 光重合型レジンライナーによる象牙細管封鎖効果 ---,
第93回日本歯科保存学会秋季学会, 1990年9月.

研究会・報告書

湯本浩通, 太良尾浩生, 平尾功治, 富永敏彦, 坂東直樹, 高比良一輝, 榎本崇宏, 芥川正武, 池原敏孝, 松尾敬志, 木内陽介 :
電磁波照射による骨芽細胞培養培地内の電流密度分布とVEGF産生との関連,
LED総合フォーラム2020 in 徳島, 2020年2月. 村上圭史, 小野恒子, 谷口和子, 松尾敬志, 三宅洋一郎 :
付着緑膿菌における抗菌薬抵抗性変異株の分離とその性状,
Bacterial Adherence & Biofilm, Vol.13, 23-25, 1999年1月. 村上欣也, 弘田克彦, 根本謙, 小野恒子, 松尾敬志, 三宅洋一郎 :
Streptococcus intermediusの抗sialyl-Lewis^x (CD15s)抗体誘導能,
Bacterial Adherence & Biofilm, Vol.11, 14-20, 1997年1月. 弘田克彦, 市川哲雄, 柏原稔也, 根本謙, 小野恒子, 村上欣也, 松尾敬志, 松本直之, 三宅洋一郎 :
Candida albicans biofilm modelの作製と電解水の抗biofilm効果,
Bacterial Adherence & Biofilm, Vol.10, 30-35, 1996年1月. 菅俊行, 野口直人, 松尾敬志, 吉本勝彦, 市川哲雄 :
日本大学松戸歯学部臨床実習視察報告,
四国歯学会雑誌, Vol.26, No.2, 81-89, 徳島, 2014年2月.

(徳島大学機関リポジトリ): ● Metadata: 109848

(徳島大学機関リポジトリ: 109848) 大石慶二, 竹内裕久, 松尾敬志, 吉本勝彦, 市川哲雄 :
広島大学歯学部臨床実習視察報告,
四国歯学会雑誌, Vol.26, No.2, 71-79, 2014年2月.

(徳島大学機関リポジトリ): ● Metadata: 109847

(徳島大学機関リポジトリ: 109847) 尾崎和美, 松尾敬志 :
Bacterial Viability Assay at an Electron Microscopic Leved,
カリオロージに基づく齲蝕の診断法および治療法の開発, 51-58, 2005年3月. 尾崎和美, 松尾敬志 :
PCR Amplificcation of Target DNA Extracted From Bacterial Cells Embedded in Plastic Material for Electron Microscopy,
カリオロージに基づく齲蝕の診断法および治療法の開発, 29-49, 2005年3月.

(キーワード): Bactrerial DNA / Semiquantitative PCR / Embedding-Material

尾崎和美, 松尾敬志 :
電顕用樹脂埋試料から抽出した細菌の16SrDNAを利用した象牙齲蝕病巣深部の細菌の同定,
カリオロジーに基づく齲蝕の診断法および治療法の開発, 15-27, 2005年3月.

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 訪問・在宅に向けた新しい概念の保存治療法の開発―高周波/電磁波などを応用して― (研究課題/領域番号: 17K11708 )
エピジェネティクス・転写後発現調節機構の解析による新規歯髄温存・石灰化療法の開発 (研究課題/領域番号: 15K11117 )
高周波/電磁波治療の深在性齲蝕治療への応用―訪問・在宅治療を目指して― (研究課題/領域番号: 26462885 )
菌体外ＤＮＡ・蛋白複合体を標的とした口腔バイオフィルム感染症の予防と治療法の開発 (研究課題/領域番号: 24592872 )
ミニマムインターベンションの概念と齲蝕病態に基づく新規の包括的齲蝕治療法の開発 (研究課題/領域番号: 23390435 )
難治性根尖性歯周炎と根尖孔外バイオフィルムに対する電磁・高周波の歯内療法への応用 (研究課題/領域番号: 21592423 )
根面齲蝕の病態・病因の解析に基づく新しい診断法と治療法の開発 (研究課題/領域番号: 20592229 )
遺伝子工学的手法を応用した難治性根尖性歯周炎症例の細菌侵襲の実態の解析 (研究課題/領域番号: 18592092 )
歯原性上皮細胞および歯髄細胞を用いた歯質再建のための細胞療法の開発 (研究課題/領域番号: 17659598 )
象牙細管侵入細菌の菌体内多糖合成に関する動的解析 (研究課題/領域番号: 17591993 )
齲蝕象牙質の細管侵入細菌におけるスクロース代謝の動的解析 (研究課題/領域番号: 15592021 )
カリオロジーに基づく齲蝕の診断法および治療法の開発 (研究課題/領域番号: 14207081 )
微小組織切片中の核酸を利用した露髄部の細菌種の同定および炎症歯髄の病態解析 (研究課題/領域番号: 13672000 )
要介護者における口腔ケアの効果,特に嚥下性肺炎予防に対する効果に関する研究 (研究課題/領域番号: 12672275 )
ヒト根尖性歯周炎における根尖孔外への細菌侵襲の実態と宿主の応答 (研究課題/領域番号: 12671853 )
象牙細管内の環境をシミュレーションした露出歯根象牙質齲蝕の誘発モデルの確立 (研究課題/領域番号: 11671896 )
ヒト感染根管の最近侵襲の実態と根尖部における宿主・細菌の相互関係の解析 (研究課題/領域番号: 10470406 )
歯内疾患の実態調査と治癒に関する研究 (研究課題/領域番号: 10307048 )
口腔内生体防御成分の分析による口腔疾患の予防法と看護に関する研究 (研究課題/領域番号: 09672401 )
ヒト歯周炎における炎症浸潤細胞の接着機構の研究 (研究課題/領域番号: 08672193 )
In vivoにおける歯根部齲蝕の誘発とその細菌学的ならびに形態学的解析 (研究課題/領域番号: 07672078 )
歯周組織破壊の進行過程における免疫担当細胞の動態 (研究課題/領域番号: 06671916 )
ヒトの歯周ポケット底部における宿主-細菌相互作用の解析 (研究課題/領域番号: 06454541 )
歯髄炎の非可逆性化に関与する炎症機構の解析 (研究課題/領域番号: 04671162 )
歯周ポケットにおける歯周病関連細菌の局在性の検索とその組織破壊との関連性 (研究課題/領域番号: 04454472 )
歯周炎における活動期病変の診断法 (研究課題/領域番号: 02454435 )
歯周疾患における線維芽細胞と免疫担当細胞間相互作用に関する研究 (研究課題/領域番号: 01571042 )
慢性根尖性歯周炎成立機序に関する免疫学的研究 (研究課題/領域番号: 62480385 )
研究者番号（30173800）による検索

その他: 研究者総覧に該当データはありませんでした。

研究者総覧で最新データを確認する

2024年4月26日更新

専門分野・研究分野: 歯科保存学 (Conservative Dentistry)
所属学会・所属協会: 日本歯科保存学会
日本歯周病学会
日本接着歯学会
日本臨床歯内療法学会
四国歯学会
国際歯科研究学会
米国歯内療法学会
委員歴・役員歴: 日本歯科保存学会 (理事 [2011年4月〜2013年3月])
日本歯周病学会 (評議員)
日本接着歯学会 (理事)
日本臨床歯内療法学会 (理事 [2010年1月〜2011年12月])
四国歯学会 (理事)
受賞: 1998年1月, 三木康楽賞 (財団法人三木康楽会)
活動: 研究者総覧に該当データはありませんでした。

リサーチマップで最新データを確認するＪグローバルで最新データを確認する

2024年4月28日更新

2024年4月27日更新

Ｊグローバル

Jグローバル最終確認日: 2024/4/27 01:28
氏名（漢字）: 松尾敬志
氏名（フリガナ）: マツオタカシ
氏名（英字）: Matsuo Takashi
所属機関: 徳島大学教授

リサーチマップ

researchmap最終確認日: 2024/4/28 01:58
氏名（漢字）: 松尾敬志
氏名（フリガナ）: マツオタカシ
氏名（英字）: Matsuo Takashi
プロフィール: リサーチマップAPIで取得できませんでした。
登録日時: 2006/1/7 00:00
更新日時: 2022/9/10 19:16
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所属ID: 0344020000
所属: 徳島大学
部署: 大学院ヘルスバイオサイエンス研究部歯科保存学分野
職名: 教授
学位: 歯学博士
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ＫＡＫＥＮで最新データを確認する

2024年4月27日更新

研究者番号: 30173800

所属（現在）: KAKEN APIで取得できませんでした。

所属（過去の研究課題 情報に基づく）*注記: 2019/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 非常勤講師
2018/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 教授
2016/4/1 – 2017/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学系), 教授
2015/4/1 : 徳島大学, 大学院医歯薬学研究部, 教授
2014/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2012/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 教授
2007/4/1 – 2011/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
2005/4/1 – 2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授
2004/4/1 – 2005/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授
1996/4/1 – 2003/4/1 : 徳島大学, 歯学部, 教授
1998/4/1 : 徳島大学, 歯学科, 教授
1993/4/1 – 1995/4/1 : 徳島大学, 歯学部, 助教授
1992/4/1 : 徳島大学, 歯学部・附属病院, 講師
1990/4/1 – 1992/4/1 : 徳島大学, 歯学部附属病院, 講師
1988/4/1 – 1989/4/1 : 大阪大学, 歯学部, 助手

審査区分/研究分野

研究代表者

医学 / 歯学 / 保存治療系歯学
生物系 / 医歯薬学 / 歯学 / 保存治療系歯学

研究代表者以外

医学 / 歯学 / 保存治療系歯学
生物系 / 医歯薬学 / 歯学 / 保存治療系歯学
医学 / 医学一般 / 看護学
医学 / 医学一般 / 基礎・地域看護学

キーワード

研究代表者

可逆性歯髄炎 / 非可逆性歯髄炎 / 歯髄血液 / 免疫グロブリン / プロスタグランジンE_2 / インターロイキン / 診断指標 / ELISA / 歯周組織破壊 / 免疫組織 / 多重染色 / 線維芽細胞 / 炎症浸潤細胞 / T細胞 / B細胞 / 歯根部齲蝕 / 齲蝕関連細菌 / 菌体内多糖 / 免疫電顕染色 / 歯周炎 / リンパ球 / 接着分子 / Woodruff assay / 再生医学 / 幹細胞 / クローン / 分化 / 細胞外基質 / 根面齲蝕 / ミュータンス菌 / 再石灰化 / 歯髄炎 / カテキン / 象牙細管侵入細菌 / 歯髄応答 / 自然免疫 / 病原体特異的分子パターン / パターン認識受容体 / 茶カテキン / 自然免疫機構 / 齲蝕 / ミニマムインターベンション / 高周波/電磁波 / アンモニウムヘクサフルオロシリケート / 齲蝕治療法 / ミニマルインターベンション / 象牙質再石灰化法 / 電磁波/高周波 / 自然免疫応答 / ミニマルインターベンション（MI） / フッ化ジアミンシリケート / ポリフェノール / 深在性齲蝕 / 歯髄細胞 / 象牙芽細胞 / 高周波/電磁波治療 / 電磁波・高周波 / 遺伝子発現 / Streptococcus mutans / 殺菌効果 / 骨芽細胞 / 成長因子 / 感染根管 / 免疫染色 / 象牙細管 / 口腔内細菌 / 根管壁 / 根尖病巣 / 根管拡大 / 根尖部 / バイオフィルム / 局在 / 難治性根尖性歯周炎 / 歯根部象牙細管 / 侵入 / Infected root canal / Immunohistology / Dentinal tubules / Bacteria / Pulpal wall / Periapical lesion / Canal enlargement / 根尖性歯周炎 / 根尖孔外 / 細菌 / 歯根部象牙質 / 走査型電子顕微鏡 / 根管治療 / 根尖孔 / apical periodontitis / extra-radical area / bacteria / root dentin / immunohistology / SEM / refractory apical periodontitis / infected root canal treatment / 診断 / 治療法 / rDNA / 色彩評価 / pH-イメージ / カリエスリスク / 診断法 / 齲蝕除去 / DIAGNOdent / 齲蝕溶解液 / リスク評価 / 疫学的解析 / caries / diagnosis / treatment / biofilm / color / pH-imaging / caries risk / 難治症例 / 遺伝子増幅 / 細菌感染 / 特異的遺伝子配列 / Periapical Periodontitis / Refractory case / Gene Amplification / Bacterial Infection / Specific Gene Sequence / う蝕治療 / 初期歯髄炎 / 在宅・訪問診療 / 深在性う蝕 / 訪問・在宅治療 / 在宅・訪問歯科治 / 齲蝕治療 / 健康寿命 / 在宅・訪問歯科治療

研究代表者以外

線維芽細胞 / HLAーDR抗原 / インタ-フェロンガンマ / 酵素抗体法 / 歯内療法学 / 根尖性歯周炎 / 電磁・高周波 / 細菌 / 骨芽細胞 / 遺伝子発現 / 成長因子 / シグナル伝達 / 歯内療法 / マイクロアレイ / オステオポンティン / バイオフィルム / 歯学 / 感染症 / 細胞・組織 / 遺伝子 / 糖鎖 / 診断方法 / 歯周炎 / 活動期病変 / 線形判別分析 / 歯周ポケット内細菌 / 歯肉溝浸出液 / 歯肉縁下プラ-ク / 歯肉溝滲出液 / Diagnostic method / Periodontitis / Periodontal disease activity / Linear discriminant analysis / Subgingival plaque / Gingival crevicular fluid / 歯周病関連細菌 / プラーク細菌 / ヒト歯周ポケット / 局在性 / 免疫組織学的検索 / 細菌レクチン / B細胞マイトジェン / 付着因子 / 免疫組織学 / 微細形態学 / Periodontal disease-associated bacteria / Plaque bacteria / Human periodontal pocket / Localization / Immunohistological observation / Bacterial lectin / B cell mitogen / 歯周ポケット / 歯肉縁下プラーク / スピロヘータ / 歯周組織破壊 / T細胞 / B細胞 / スペロヘ-タ / 歯周病原細菌 / アポトーシス / Periodontal pocket / Subgingival plque / Spirochetes / Periodontal tissue destruction / T cell / B cell / 口腔細菌 / 嚥下性肺炎 / 歯科的疾患 / 生体防御 / 口腔ケア / 含嗽 / microorganisms in the oral cavity / aspiration pneumonia / oral deseases / host defence / oral cavity care / mouth wash / 歯内疾患 / 歯内歯周疾患 / 適応症 / 根管内容物 / 根管充填の過不足 / 予後経過 / 遠隔成績 / 感染根管 / 難治性歯内疾患 / 再植歯 / 根管充填 / 根管滲出液 / 歯髄疾患 / 感染根管治療 / 根管治療薬 / 予後評価 / 診断システム / endodontic disease / periapical lesion / endodontic periodontic lesion / case selection / periapical exudate / failures by canal space / prognosis / follow-up study / 齲蝕関連細菌 / 歯髄内圧 / 菌体内多糖 / 菌体関連多糖 / Caries-associated bacteria / Pulpal pressure / Intracellular polysaccharide / ORAL CAVITY BACTERIA / ASPIRATION PNEUMONIA / DENTAL DISEASE / HOST DEFENSE / ORAL CARE / 象牙質齲蝕 / 16S rDNA / 電顕用樹脂 / TEM-in situ hybridization / RT-PCR / Toll-like receptor / Dentinal caries / Embedding material for TEM / Streptococcus mutans / glucosyltransferase / mRNA / グルカン産生能 / glucan synthesis / Quorum Sensing System / Quorum sensing system / 根尖病巣 / 免疫学的研究 / 免疫担当細胞 / インターロイキンー1 / 慢性根尖性歯周炎 / Immunological study / Periapical lesion / 象牙芽細胞 / 自然免疫 / ポリフェノール / ケモカイン / VEGF

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松尾 敬志

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松尾敬志