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富山芳信

徳島大学

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2026年4月21日更新

職名: 准教授, 助教授, 講師
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学歴: 1986/3: 徳島大学医学部医学科卒業
学位: 博士 / 博士(医学) (徳島大学) (1994年3月)
職歴・経歴: 1986/5: 徳島大学医員医学部附属病院
1986/7: 高知市立市民病院研修医
1988/4: 徳島市民病院医師
1989/7: 高知市立市民病院医師(麻酔科)
1991/7: 徳島大学医学部附属病院医員(麻酔科)
1992/7: 徳島大学医学部附属病院助手(麻酔科)
1997/3: 徳島大学医学部附属病院講師(麻酔科)
1997/5: University of Iowa, Department of Anesthesiology, Associate Visiting Scholar
1999/4: 徳島大学医学部附属病院講師(麻酔科)
2001/10: 徳島大学医学部講師(麻酔学講座)
2002/3: 徳島大学医学部講師(侵襲病態制御医学分野)
2004/4: 徳島大学大学院講師(侵襲病態制御医学分野)

専門分野・研究分野: 麻酔科学 (Anesthesiology)

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2026年4月21日更新

専門分野・研究分野: 麻酔科学 (Anesthesiology)
担当経験のある授業科目: 周産期・小児・女性生殖器コース (学部)
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2026年4月21日更新

専門分野・研究分野: 麻酔科学 (Anesthesiology)

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著書

富山芳信, 田中克哉 :
抜管の基準，手術室退出の基準,
診断と治療社, 2018年12月. 富山芳信, 大下修造 :
麻酔中の循環系の変化,
真興交易(株)医書出版部, 東京, 2002年.

論文

Yako Matsumoto, Michiko Kinoshita, Yoshinobu Tomiyama and Katsuya Tanaka :
Earthquake Preparedness for Operating Room Staff: Lessons Learned From Experiences and the Literature,
Anesthesia & Analgesia, 2024.

(要約): Earthquakes pose hazards to health care workers and patients in operating rooms. Proactive planning based on insights gained from past experiences is crucial for enhancing safety. Through a comprehensive literature review, we summarize challenges and lessons learned from real earthquake events to inform the development of effective safety measures in operating rooms. Additionally, we discuss the anesthesiologist's role in crisis management.
(徳島大学機関リポジトリ): ● Metadata: 2012318
(出版サイトへのリンク): ● Publication site (DOI): 10.1213/ANE.0000000000007250
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 39504302; ● Search Scopus @ Elsevier (PMID): 39504302; ● Search Scopus @ Elsevier (DOI): 10.1213/ANE.0000000000007250

(徳島大学機関リポジトリ: 2012318, DOI: 10.1213/ANE.0000000000007250, PubMed: 39504302) 山田将之, 四宮加容, 大串陽子, 富山芳信, 湯浅真由, 三田村佳典 :
全身麻酔手術中にラテックスアレルギーによるアナフィラキシーショックをきたした一例,
臨床眼科, 73, 4, 523-527, 2019年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11477/mf.1410213121
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1523106603346768384; ● Search Scopus @ Elsevier (DOI): 10.11477/mf.1410213121

(DOI: 10.11477/mf.1410213121, CiNii: 1523106603346768384) 富山芳信 :
スガマデクスナトリウム(MK-8616, Org25969)の筋弛緩拮抗作用ー深い筋弛緩状態からの回復ー,
麻酔, 63, 10, 1083-1088, 2014年.

(要約): Efficacy and safety of sugammadex in reversing neuromuscular block induced by rocuronium or vecuronium were investgated in Japanese patients. We studied 99 Japanese patients undergoing surgery requiring general anesthesia. Patients were allocated randomly to receive intubation dose of rocuronium or vecuronium. During surgery, patients received additional dose of rocuronium or vecuronium for maintenance of deep block. At 1-2 PTC, 0.5-8.0 mg . kg-1 of sugammadex was administered. The neuromuscular block was monitored with acceleromyography using TOF stimuli. Sevoflurane was administered to all treatment groups after intubation. For the rocuronium-induced neuromuscular block, the mean recovery time of the T4/T1 ratio to 0.9 decreased from 66.9 min in the sugammadex 0.5 mg kg-1 group to 1.3 min in the sugammadex 8.0 mg kg-1 group. For the vecuronium-induced neuromuscular block it decreased from 79.5 min in the sugammadex 0.5 mg . kg-1 group to 2.9 min in the sugammadex 8.0 mg . kg-1 group. No clinical evidence of recurarization or residual curarization was observed. The efficacy and safety of sugammadex were confirmed in Japanese surgical patients for reversal from deep block.
(キーワード): スガマデクス / 非脱分極性筋弛緩薬 / ロクロニウム / ベクロニウム / 筋弛緩回復薬 / sugammadex / non-depolarizing muscle relaxant / rocuronium / vecuronium / selective relaxant binding agent
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25693333; ● CiNii @ 国立情報学研究所 (CRID): 1520572359069035776; ● Search Scopus @ Elsevier (PMID): 25693333

(PubMed: 25693333, CiNii: 1520572359069035776) Yoshinobu Tomiyama, Sachiyo Higashijima, Katsuyoshi Kume and Naohiro Oshita :
Trends in electrocardiographic R-wave amplitude during intraoperative pneumothorax,
The Journal of Medical Investigation : JMI, 61, 3,4, 442-445, 2014.

(要約): Tension pneumothorax is a rare but potentially life-threating complication of laparoscopic fundoplication. Electrocardiogram (ECG) changes may be used in the diagnosis of intraoperative tension pneumothorax. This case study examines a pediatric patient who underwent laparoscopic fundoplication. Sudden decreases in oxygen saturation were observed during dissection, although the patient's decrease in blood pressure was less marked. Manual ventilation with high inspiratory pressure and inspiratory pause improved oxygenation. The amplitude of the R-wave decreased from 0.8 mV to 0.3 mV in 5 seconds. Twenty minutes later, oxygen saturation decreased again, the R-wave amplitude decreased from 0.3 mV to 0.1 mV in 1 second, and the decrease in blood pressure was marked. Manual ventilation with high inspiratory pressure improved oxygenation, blood pressure, and R-wave amplitude within two minutes. After conversion to open surgery, the cardiorespiratory condition gradually improved, but the R-wave amplitude did not fully recover, even at the end of surgery. Right-side pneumothorax was subsequently confirmed by postoperative chest X-ray. Chest drains were inserted after surgery. This case suggests that trends in R-wave amplitude are potential indicators of intraoperative tension pneumothorax.
(徳島大学機関リポジトリ): ● Metadata: 2002846
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.61.442
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25264070; ● Search Scopus @ Elsevier (PMID): 25264070; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.61.442

(徳島大学機関リポジトリ: 2002846, DOI: 10.2152/jmi.61.442, PubMed: 25264070) 大下修弘, 髙田香, 河原富也, 富山芳信, 川人伸次 :
術中冠攣縮が疑われた糖尿病患者の1症例,
臨床麻酔, 38, 4, 615-618, 2014年.

(キーワード): 冠攣縮 / 糖尿病

Kaori Takata, Yoshinobu Tomiyama, Katsuya Tanaka and Shuzo Oshita :
Cardioprotective effects of hyperkalemia during smulated ischemia/reperfusion in neonatal rat cardiomyocytes - Preservation of Na+/K+-ATPase activity -,
The Journal of Medical Investigation : JMI, 60, 1,2,, 66-76, 2013.

(要約): Hyperkalemia has multimodal effects on myocardial protection during ischemia/reperfusion. The preservation of Na(+)/K(+)-ATPase activity induced by hyperkalemia may have critical impact on myocardial protection. To elucidate the roles of hyperkalemia (16 mM) and Na(+)/K(+)-ATPase inhibition (100 µM ouabain) in myocardial protection during simulated ischemia (5 mM NaCN and 5.5 mM 2-deoxyglucose)/reperfusion, we measured loss of membrane integrity and bleb formation using a vital dye calcein AM in cultured neonatal rat cardiomyocytes. The control perfusate was switched to treatment solution for 15 min, followed by reperfusion for 30 min. In a second set of experiments, myocardial excitability and diastolic intracellular calcium ion concentration ([Ca(2+)]i) were measured during a 45-min treatment using a calcium-sensitive fluorescent dye fluo-4 AM. Simulated ischemia/reperfusion under ouabain treatment induced loss of membrane integrity, which was suppressed by hyperkalemia. Simulated ischemia/reperfusion induced bleb formation, which was accelerated by ouabain. Hyperkalemia delayed and inhibited the increase in diastolic [Ca(2+)]i induced by simulated ischemia. Furthermore, hyperkalemia almost completely inhibited the effects of ouabain on the diastolic [Ca(2+)]i during ischemia. These results suggest that hyperkalemia during ischemia is cardioprotective against ischemia/reperfusion insults and that hyperkalemia inhibits the effects of ouabain during ischemia.
(キーワード): Animals / Animals, Newborn / Calcium / Cells, Cultured / Hyperkalemia / Myocardial Ischemia / Myocardial Reperfusion / Myocytes, Cardiac / Ouabain / Rats / Rats, Sprague-Dawley / Sodium-Potassium-Exchanging ATPase
(徳島大学機関リポジトリ): ● Metadata: 2004487
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.60.66
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23614914; ● Search Scopus @ Elsevier (PMID): 23614914; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.60.66

(徳島大学機関リポジトリ: 2004487, DOI: 10.2152/jmi.60.66, PubMed: 23614914) Naohiro Ohshita, Katsuya Tanaka, Yoko Sakai, Toshiko Katayama, Yoshinobu Tomiyama, Shuzo Ohsita and Yasuo M. Tsutsumi :
Anesthesia for deep brain stimulation in a patient with X-linked dystonia-parkinsonism/Lubag disease.,
The Journal of Medical Investigation : JMI, 60, 1-2, 146-148, 2013.

(要約): Lubag disease is a genetic X-linked dystonia-parkinsonism syndrome afflicting Filipino men. This disease is characterized by dystonia dominating the first 10-15 years of the disorder, which is associated with or replaced by parkinsonian features in later years of life. A 49-year-old man with Lubag disease underwent general anesthesia for deep brain stimulation (DBS) surgery. Anesthesia was maintained mainly with propofol, remifentanil, rocuronium bromide, and sevoflurane. During magnetic resonance imaging, the patient was anesthetized with midazolam, fentanyl, and rocuronium bromide. The surgery was completed safely using these anesthetic agents. After DBS, some symptoms including involuntary movement improved within 10 days.
(徳島大学機関リポジトリ): ● Metadata: 2002254
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.60.146
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23614923; ● Search Scopus @ Elsevier (PMID): 23614923; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.60.146

(徳島大学機関リポジトリ: 2002254, DOI: 10.2152/jmi.60.146, PubMed: 23614923) 大下修弘, 堤保夫, 笠井飛鳥, 曽我朋宏, 金村朋美, 片山俊子, 井関明生, 富山芳信, 田中克哉 :
スガマデクス投与後にアナフィラキシー様反応を呈した2症例,
麻酔, 61, 11, 1261-1264, 2012年.

(要約): Anaphylaxis during anesthesia is a rare but life-threatening event. Sugammadex is a recently introduced drug that was specifically designed for the reversal of rocuroium and vecuronium-induced neuromuscular block. We describe the cases of a 74-year-old man and a 29-year-old man who developed an anaphylactoid reaction to sugammadex, presenting with cardiovascular collapse. Initial management consisted of fluid administration and intermittent i.v. ephedrine, epinephrine, and hydrocortisone. The patients made uncomplicated recovery and were discharged.
(キーワード): スガマデクス / アナフィラキシー様反応 / sugammadex / anaphylactoid reaction
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23236935; ● CiNii @ 国立情報学研究所 (CRID): 1520290883431817856; ● Summary page in Scopus @ Elsevier: 2-s2.0-84870043010

(PubMed: 23236935, CiNii: 1520290883431817856, Elsevier: Scopus) 大下修弘, 堤保夫, 大下修造, 髙田香, 富山芳信, 田中克哉 :
筋萎縮性側索硬化症患者の麻酔経験,
麻酔, 61, 9, 1006-1008, 2012年.

(要約): A 31-year-old woman with amyotrophic lateral sclerosis (ALS) with respiratory muscle paralysis was scheduled for tracheotomy. After applying standard neuromuscular monitoring devices, general anesthesia was induced and maintained with propofol, remifentanil, rocuronium, and sevoflurane. Sugammadex is a potent agent for reversal of neuromuscular blockade by rocuronium. The patient emerged from general anesthesia smoothly using sugammadex; however, assisted respiration was continued for possible prolongation of the effect of muscle relaxant. The postoperative course was uneventful, and she was discharged without any discomfort.
(キーワード): 筋萎縮性側索硬化症 / ロクロニウム / スガマデクス / amyotrophic lateral sclerosis / rocuronium / sugammadex
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23012840; ● CiNii @ 国立情報学研究所 (CRID): 1520290883032602496; ● Summary page in Scopus @ Elsevier: 2-s2.0-84866311715

(PubMed: 23012840, CiNii: 1520290883032602496, Elsevier: Scopus) 河野裕明, 真鍋佐和, 髙田香, 曽我朋宏, 富山芳信, 大下修造 :
胸部大動脈瘤により高度気管狭窄を呈した症例の麻酔経験,
麻酔, 60, 10, 1195-1198, 2011年.

(要約): Tracheobronchial compression is a well-recognized complication of thoracic aortic aneurysm. We describe the anesthetic management of a patient with severe tracheal stenosis due to thoracic aortic aneurysm. An 81-year-old woman was scheduled for endovascular aortic stent graft placement. Computed tomographic (CT) scans showed that the narrowest diameter of the trachea was 3 x 18 mm. Awake fiberoptic intubation was selected for anesthesia induction, and percutaneous cardiopulmonary support (PCPS) was ready to be established prior to induction of anesthesia. We successfully inserted ID 6.0 mm spiral tube beyond the tracheal compression using bronchoscope and induced hypotension. The operation was completed successfully without any adverse events. We conclude that, in patients with thoracic aortic aneurysm, careful attention should be paid not only to circulation but to respiration.
(キーワード): 胸部大動脈瘤 / 気管狭窄 / 意識下挿管 / 気管支鏡 / 人為的低血圧 / thoracic aortic aneurysm / tracheal stenosis / awake intubation / bronchoscope / induced hypotension
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22111364; ● CiNii @ 国立情報学研究所 (CRID): 1520290883004924032; ● Summary page in Scopus @ Elsevier: 2-s2.0-80054842284

(PubMed: 22111364, CiNii: 1520290883004924032, Elsevier: Scopus) 富山芳信, 野村佳代, 角田奈美 :
重症筋無力症患者にロクロニウムとスガマデクスを使用した2症例,
日本臨床麻酔学会誌, 31, 5, 791-797, 2011年.

(キーワード): Myasthenia gravis / Sugammadex / Rocuronium / Thymectomy / Muscle relaxant
(出版サイトへのリンク): ● Publication site (DOI): 10.2199/jjsca.31.791
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1572824499837955968; ● Search Scopus @ Elsevier (DOI): 10.2199/jjsca.31.791

(DOI: 10.2199/jjsca.31.791, CiNii: 1572824499837955968) Yasuo M. Tsutsumi, Yoshinobu Tomiyama, Yousuke T. Horikawa, Yoko Sakai, Naohiro Ohshita, Katsuya Tanaka and Shuzo Oshita :
General anesthesia for electroconvulsive therapy with Brugada electrocardiograph pattern.,
The Journal of Medical Investigation : JMI, 58, 3-4, 273-276, 2011.

(要約): Brugada syndrome is characterized by an electrocardiograph pattern of right bundle-branch block and has an increased risk for cardiac arrest due to malignant arrhythmia. We describe the successful anesthetic management for electroconvulsive therapy in a patient with Brugada electrocardiograph pattern. Patients with Brugada ECG pattern are not recommended to use neostigmine which augments ST elevation. Sugammadex was administered as a neuromuscular reversal agent in this case. Sugammadex provides rapid reversal of profound rocuronium-induced neuromuscular blockade under propofol anesthesia.
(キーワード): Anesthesia, General / Brugada Syndrome / Electrocardiography / Electroconvulsive Therapy / Humans / Male / Middle Aged / gamma-Cyclodextrins
(徳島大学機関リポジトリ): ● Metadata: 2001577
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.58.273
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21921431; ● Search Scopus @ Elsevier (PMID): 21921431; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.58.273

(徳島大学機関リポジトリ: 2001577, DOI: 10.2152/jmi.58.273, PubMed: 21921431) 大下修弘, 富山芳信, 堤保夫, 角田奈美, 曽我朋宏, 井関明生, 大下修造, 田中克哉 :
ベッカー型筋ジストロフィ症患者の麻酔経験,
麻酔, 60, 8, 950-952, 2011年.

(要約): We experienced anesthetic management of a patient with Becker muscular dystrophy. He had advanced dilated cardiomyopathy and high serum CK in the preoperative examinations. Anesthesia was planned to avoid triggering malignant hyperthermia or rhabdomyolysis and hemodynamic changes. Propofol, remifentanil and a minimum dose of rocuronium bromide were used for anesthetic induction and maintainance. Arterial pressure, cardiac output and stroke volume variation were monitored by Flotrac sensor. There were no adverse events observed during the anesthetic management. In conclusion, total intravenous anesthesia with the administration of rocuronium and circulatory monitoring by Flotrac sensor could be safe and efficient for anesthetic management of patients with Becker muscular dystrophy.
(キーワード): ベッカー型筋ジストロフィ / 拡張型心筋症 / 悪性高熱症 / フロートラックセンサー / ロクロニウム / Becker muscular dystrophy / dilated cardiomyopathy / malignant hyperthermia / Flotrac sensor / rocuronium
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21861423; ● CiNii @ 国立情報学研究所 (CRID): 1520290883351512832; ● Summary page in Scopus @ Elsevier: 2-s2.0-80052046221

(PubMed: 21861423, CiNii: 1520290883351512832, Elsevier: Scopus) 大下修弘, 富山芳信, 井関明生, 河野裕明, 角田奈美, 堤保夫, 大下修造 :
Angelman症候群の麻酔経験,
麻酔, 59, 4, 484-486, 2010年.

(要約): Angelman syndrome is a hereditary disease described by Angelman. The clinical features of Angelman syndrome are characterized by mental retardation, puppet-like ataxia, easily excitable personality, seizures, paroxysmal laughter, strabismus and macroglossia. A 4-year-old girl with Angelman syndrome underwent strabismus repair under general anesthesia. Anesthesia was slowly induced with sevoflurane in oxygen and maintained with air, oxygen, propofol and remifentanil. Tracheal intubation was performed after administration of rocuronium. During and after anesthesia, no adverse events regarding circulatory and respiratory systems occurred. However, this case demonstrates that it is necessary to pay attention to airway troubles including the difficulty of tracheal intubation, management of body temperature and chronotropic action or respiratory depression by anesthetic agents.
(キーワード): *Angelman症候群(合併症)
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20420140; ● CiNii @ 国立情報学研究所 (CRID): 1520290883021246848; ● Summary page in Scopus @ Elsevier: 2-s2.0-77951582562

(PubMed: 20420140, CiNii: 1520290883021246848, Elsevier: Scopus) Mikiyo Yamaguchi, Yoshinobu Tomiyama, Tosiko Katayama, Hiroshi Kitahata and Shuzo Oshita :
Involvement of adenosine triphosphate-sensitive potassium channels in the response of membrane potential to hyperosmolality in cultured human aorta endothelial cells.,
Anesthesia & Analgesia, 100, 2, 419-426, 2005.

(要約): The membrane potential of endothelial cells is an important determinant of endothelial functions, including regulation of vascular tone. We investigated whether adenosine triphosphate-sensitive potassium (K(ATP)) channels were involved in the response of membrane potential to hyperosmolality in cultured human aorta endothelial cells. The voltage-sensitive fluorescent dye, bis-(1,3-diethylthiobarbiturate)trimethine oxonol, was used to assess relative changes in membrane potential semiquantitatively. To investigate the effect of mannitol-, sucrose-, and NaCl-induced hyperosmolality on membrane potential, cells were continuously perfused with Earle's balanced salt solution (285 mOsm/kg H(2)O) containing 200 nM bis-(1,3-diethylthiobarbiturate)trimethine oxonol and exposed to 315 and 345 mOsm/kg H(2)O hyperosmotic medium sequentially in the presence and absence of 1 muM glibenclamide, a well-known K(ATP) channel blocker. Hyperosmotic mannitol significantly induced hyperpolarization of the endothelial cells, which was prevented by 1 microM glibenclamide (n = 6). Estimated changes of membrane potential at 315 and 345 mOsm/kg H(2)O were 13 +/- 8 and 21 +/- 8 mV, respectively. Hypertonic sucrose induced similar changes. However, although hypertonic saline also significantly induced hyperpolarization of the endothelial cells (n = 6), the hyperpolarization was not prevented by 1 muM glibenclamide. In conclusion, K(ATP) channels may participate in hyperosmotic mannitol- and sucrose-induced hyperpolarization, but not in hypertonic saline-induced hyperpolarization in cultured human aorta endothelial cells.
(キーワード): ATP-Binding Cassette Transporters / Aorta / 校正 (calibration) / Cells, Cultured / Cromakalim / Diuretics, Osmotic / Endothelial Cells / Fluorescent Dyes / Glyburide / Humans / Hypoglycemic Agents / Indicators and Reagents / KATP Channels / Mannitol / Membrane Potentials / Osmolar Concentration / Perfusion / Potassium Channels / Potassium Channels, Inwardly Rectifying / Sodium Chloride / Sucrose / Thiobarbiturates / Vasodilator Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1213/01.ANE.0000143350.82645.5B
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15673869; ● Search Scopus @ Elsevier (PMID): 15673869; ● Search Scopus @ Elsevier (DOI): 10.1213/01.ANE.0000143350.82645.5B

(DOI: 10.1213/01.ANE.0000143350.82645.5B, PubMed: 15673869) Takashi Kawano, Shuzo Oshita, Akira Takahashi, Y Tsutsumi, Katsuya Tanaka, Yoshinobu Tomiyama, Hiroshi Kitahata and Yutaka Nakaya :
Molecular mechanisms underlying ketamine-mediated inhibition of sarcolemmal adenosine triphosphate-sensitive potassium channels.,
Anesthesiology, 102, 1, 93-101, 2005.

(要約): Ketamine inhibits adenosine triphosphate-sensitive potassium (KATP) channels, which results in the blocking of ischemic preconditioning in the heart and inhibition of vasorelaxation induced by KATP channel openers. In the current study, the authors investigated the molecular mechanisms of ketamine's actions on sarcolemmal KATP channels that are reassociated by expressed subunits, inwardly rectifying potassium channels (Kir6.1 or Kir6.2) and sulfonylurea receptors (SUR1, SUR2A, or SUR2B). The authors used inside-out patch clamp configurations to investigate the effects of ketamine on the activities of reassociated Kir6.0/SUR channels containing wild-type, mutant, or chimeric SURs expressed in COS-7 cells. Ketamine racemate inhibited the activities of the reassociated KATP channels in a SUR subtype-dependent manner: SUR2A/Kir6.2 (IC50 = 83 microM), SUR2B/Kir6.1 (IC50 = 77 microM), SUR2B/Kir6.2 (IC50 = 89 microM), and SUR1/Kir6.2 (IC50 = 1487 microM). S-(+)-ketamine was significantly less potent than ketamine racemate in blocking all types of reassociated KATP channels. The ketamine racemate and S-(+)-ketamine both inhibited channel currents of the truncated isoform of Kir6.2 (Kir6.2DeltaC36) with very low affinity. Application of 100 mum magnesium adenosine diphosphate significantly enhanced the inhibitory potency of ketamine racemate. The last transmembrane domain of SUR2 was essential for the full inhibitory effect of ketamine racemate. These results suggest that ketamine-induced inhibition of sarcolemmal KATP channels is mediated by the SUR subunit. These inhibitory effects of ketamine exhibit specificity for cardiovascular KATP channels, at least some degree of stereoselectivity, and interaction with intracellular magnesium adenosine diphosphate.
(キーワード): ATP-Binding Cassette Transporters / Adenosine Diphosphate / Animals / COS Cells / Cercopithecus aethiops / DNA, Complementary / Excitatory Amino Acid Antagonists / KATP Channels / Ketamine / Mutation / Patch-Clamp Techniques / Potassium Channel Blockers / Potassium Channels / Potassium Channels, Inwardly Rectifying / Rats / Recombinant Fusion Proteins / Sarcolemma / Stereoisomerism / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200501000-00017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15618792; ● Search Scopus @ Elsevier (PMID): 15618792; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200501000-00017

(DOI: 10.1097/00000542-200501000-00017, PubMed: 15618792) Takashi Kawano, Shuzo Oshita, Akira Takahashi, Yasuo Tsutsumi, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda and Yutaka Nakaya :
Molecular Mechanisms of the Inhibitory Effects of Bupivacaine, Levobupivacaine, and Ropivacaine on Sarcolemmal Adenosine Triphosphate-sensitive Potassium Channels in the Cardiovascular System.,
Anesthesiology, 101, 2, 390-398, 2004.

(要約): Sarcolemmal adenosine triphosphate-sensitive potassium (KATP) channels in the cardiovascular system may be involved in bupivacaine-induced cardiovascular toxicity. The authors investigated the effects of local anesthetics on the activity of reconstituted KATP channels encoded by inwardly rectifying potassium channel (Kir6.0) and sulfonylurea receptor (SUR) subunits. The authors used an inside-out patch clamp configuration to investigate the effects of bupivacaine, levobupivacaine, and ropivacaine on the activity of reconstituted KATP channels expressed in COS-7 cells and containing wild-type, mutant, or chimeric SURs. Bupivacaine inhibited the activities of cardiac KATP channels (IC50 = 52 microm) stereoselectively (levobupivacaine, IC50 = 168 microm; ropivacaine, IC50 = 249 microm). Local anesthetics also inhibited the activities of channels formed by the truncated isoform of Kir6.2 (Kir6.2 delta C36) stereoselectively. Mutations in the cytosolic end of the second transmembrane domain of Kir6.2 markedly decreased both the local anesthetics' affinity and stereoselectivity. The local anesthetics blocked cardiac KATP channels with approximately eightfold higher potency than vascular KATP channels; the potency depended on the SUR subtype. The 42 amino acid residues at the C-terminal tail of SUR2A, but not SUR1 or SUR2B, enhanced the inhibitory effect of bupivacaine on the Kir6.0 subunit. Inhibitory effects of local anesthetics on KATP channels in the cardiovascular system are (1) stereoselective: bupivacaine was more potent than levobupivacaine and ropivacaine; and (2) tissue specific: local anesthetics blocked cardiac KATP channels more potently than vascular KATP channels, via the intracellular pore mouth of the Kir6.0 subunit and the 42 amino acids at the C-terminal tail of the SUR2A subunit, respectively.
(キーワード): ATP-Binding Cassette Transporters / Algorithms / Amides / Anesthetics, Local / Animals / Bupivacaine / COS Cells / Cercopithecus aethiops / Dose-Response Relationship, Drug / Membrane Potentials / Membrane Proteins / Patch-Clamp Techniques / Potassium Channel Blockers / Potassium Channels / Potassium Channels, Inwardly Rectifying / Receptors, Drug / Stereoisomerism / Sulfonylurea Compounds / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200408000-00020
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15277922; ● Search Scopus @ Elsevier (PMID): 15277922; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200408000-00020

(DOI: 10.1097/00000542-200408000-00020, PubMed: 15277922) Takako Masuda, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda and Shuzo Oshita :
Effect of propofol on hypotonic swelling-induced membrane depolarization in human coronary artery smooth muscle cells.,
Anesthesiology, 100, 3, 648-656, 2004.

(要約): Stretch (mechanical stress)-induced membrane depolarization of smooth muscle may contribute to basal vascular tone and myogenic control. Propofol induces vasodilation and inhibits myogenic control. Hypotonic swelling was used as a model of mechanical stress. The authors investigated the effects of propofol and 5-nitro-2-(3-phenylpropylamino)benzoic acid, a chloride channel and nonselective cation channel blocker, on hypotonicity-induced membrane depolarization in cultured human coronary artery smooth muscle cells. A voltage-sensitive fluorescent dye, bis-(1,3-diethylthiobarbiturate)trimethine oxonol, was used to assess relative changes in membrane potential semiquantitatively. The cells were continuously perfused with Earle's balanced salt solution containing 200 nM bis-(1,3-diethylthiobarbiturate)trimethine oxonol and exposed sequentially to isotonic and hypotonic medium. In a second series of experiments, the cells were exposed to hypotonic media in the presence and absence of 5-nitro-2-(3-phenylpropylamino)benzoic acid or propofol. The relative fluorescence values at 10, 20, and 30% hypotonicity were 147 +/- 29, 214 +/- 74, and 335 +/- 102% of baseline, respectively. The changes were all significantly different from the isotonic time control group. In the presence of 200 microM 5-nitro-2-(3-phenylpropylamino)benzoic acid or 0.1, 1, 10, or 100 microg/ml propofol, the relative fluorescence values at 30% hypotonicity were 87 +/- 17, 194 +/- 27, 160 +/- 18, 130 +/- 18, and 84 +/- 15%, respectively. These changes were significantly less than the 30% for the hypotonic control (246 +/- 23%). These results suggest that volume-sensitive chloride channels and nonselective cation channels may participate in hypotonicity-induced membrane depolarization and that propofol inhibits hypotonicity-induced membrane depolarization in coronary artery smooth muscle.
(キーワード): Anesthetics, Intravenous / Calibration / Cells, Cultured / Chloride Channels / Coronary Vessels / Fluorescent Dyes / Humans / Hydrogen-Ion Concentration / Hypotonic Solutions / Ion Channels / Membrane Potentials / Muscle, Smooth, Vascular / Nitrobenzoates / Perfusion / Potassium / Propofol / Sodium
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200403000-00027
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15108981; ● Search Scopus @ Elsevier (PMID): 15108981; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200403000-00027

(DOI: 10.1097/00000542-200403000-00027, PubMed: 15108981) Takashi Kawano, Shuzo Oshita, Akira Takahashi, Yasuo Tsutsumi, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda and Yutaka Nakaya :
Molecular mechanisms of the inhibitory effects of propofol and thiamylal on sarcolemmal adenosine triphosphate-sensitive potassium channels.,
Anesthesiology, 100, 2, 338-346, 2004.

(要約): Both propofol and thiamylal inhibit adenosine triphosphate-sensitive potassium (KATP) channels. In the current study, the authors investigated the effects of these anesthetics on the activity of recombinant sarcolemmal KATP channels encoded by inwardly rectifying potassium channel (Kir6.1 or Kir6.2) genes and sulfonylurea receptor (SUR1, SUR2A, or SUR2B) genes. The authors used inside-out patch clamp configurations to investigate the effects of propofol and thiamylal on the activity of recombinant KATP channels using COS-7 cells transfected with various types of KATP channel subunits. Propofol inhibited the activities of the SUR1/Kir6.2 (EC50 = 77 microm), SUR2A/Kir6.2 (EC50 = 72 microm), and SUR2B/Kir6.2 (EC50 = 71 microm) channels but had no significant effects on the SUR2B/Kir6.1 channels. Propofol inhibited the truncated isoform of Kir6.2 (Kir6.2DeltaC36) channels (EC50 = 78 microm) that can form functional KATP channels in the absence of SUR molecules. Furthermore, the authors identified two distinct mutations R31E (arginine residue at position 31 to glutamic acid) and K185Q (lysine residue at position 185 to glutamine) of the Kir6.2DeltaC36 channel that significantly reduce the inhibition of propofol. In contrast, thiamylal inhibited the SUR1/Kir6.2 (EC50 = 541 microm), SUR2A/Kir6.2 (EC50 = 248 microm), SUR2B/Kir6.2 (EC50 = 183 microm), SUR2B/Kir6.1 (EC50 = 170 microm), and Kir6.2DeltaC36 channels (EC50 = 719 microm). None of the mutants significantly affects the sensitivity of thiamylal. These results suggest that the major effects of both propofol and thiamylal on KATP channel activity are mediated via the Kir6.2 subunit. Site-directed mutagenesis study suggests that propofol and thiamylal may influence Kir6.2 activity by different molecular mechanisms; in thiamylal, the SUR subunit seems to modulate anesthetic sensitivity.
(キーワード): ATP-Binding Cassette Transporters / Anesthetics, Intravenous / Animals / Cells, Cultured / Diazoxide / Electrophysiology / Glyburide / Humans / Molecular Biology / Patch-Clamp Techniques / Pinacidil / Potassium Channels / Potassium Channels, Inwardly Rectifying / Propofol / Rats / Receptors, Drug / Thiamylal / Vasodilator Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200402000-00024
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14739809; ● Search Scopus @ Elsevier (PMID): 14739809; ● Search Scopus @ Elsevier (DOI): 10.1097/00000542-200402000-00024

(DOI: 10.1097/00000542-200402000-00024, PubMed: 14739809) Katsuya Tanaka, Hiroshi Kitahata, Shinji Kawahito, Junpei Nozaki, Yoshinobu Tomiyama and Shuzo Oshita :
Phenylephrine increases pulmonary blood flow in children with tetralogy of Fallot.,
Canadian Journal of Anaesthesia, 50, 9, 926-929, 2003.

(要約): Although it has been reported that the increase in blood pressure improves arterial oxygen saturation (SaO(2)) in children with tetralogy of Fallot, no prospective study has demonstrated that an increase in blood pressure induces an increase in pulmonary blood flow in these patients. The purpose of this study was to see whether a phenylephrine-induced increase in systemic blood pressure increased pulmonary blood flow, resulting in improved arterial oxygenation in tetralogy of Fallot. In 14 consecutive children with tetralogy of Fallot (2-32 months old), transesophageal pulsed Doppler signals of left upper pulmonary venous flow (PVF) velocity were recorded before and four minutes after 10 micro g x kg(-1) of phenylephrine i.v. Simultaneously, arterial blood gas analysis and hemodynamic measurements were performed. The minute distance (MD) was calculated as the product of the heart rate and the sum of time-velocity integrals of PVF. Phenylephrine iv increased mean arterial blood pressure from 54 +/- 8 mmHg to 73 +/- 10 mmHg. This phenylephrine-induced hypertension significantly increased SaO(2) and MD (92.0 +/- 7.5 vs 95.0 +/- 5.0% and 1318 +/- 344 vs 1533 +/- 425 cm x min(-1), respectively). There was a significant correlation (r = 0.72) between the change in MD and the change in SaO(2). Our results suggest that the phenylephrine-induced increase in systemic blood pressure produces an increase in pulmonary blood flow in tetralogy of Fallot. Our results further suggest that this increase in pulmonary blood flow is involved in the mechanism of phenylephrine-induced improvement of arterial oxygenation in tetralogy of Fallot.
(キーワード): Blood Flow Velocity / Blood Gas Analysis / 子ども (children) / Child, Preschool / Echocardiography, Doppler, Pulsed / 経食道心エコー (transesophageal echocardiography) / Female / Hemodynamics / Humans / Hypertension / 小児 (infant) / Male / Oxygen / Phenylephrine / Pulmonary Circulation / Pulmonary Veins / Tetralogy of Fallot / Vasoconstrictor Agents
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/BF03018741
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14617591; ● Search Scopus @ Elsevier (PMID): 14617591; ● Search Scopus @ Elsevier (DOI): 10.1007/BF03018741

(DOI: 10.1007/BF03018741, PubMed: 14617591) Takako Masuda, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda and Shuzo Oshita :
Propofol inhibits volume-sensitive chloride channels in human coronary artery smooth muscle cells.,
Anesthesia & Analgesia, 97, 3, 657-662, 2003.

(要約): Volume-sensitive chloride channels (VSCC) play an important role in regulation of cell volume and electrical activity. Activation of vascular smooth muscle VSCC causes smooth muscle depolarization and contraction. We investigated the effects of propofol on VSCC in cultured human coronary artery smooth muscle cells by using the chloride-sensitive dye 6-methoxy-N-ethylquinolinium (MEQ). To activate VSCC, cells were superfused for 2 min with hypotonic gluconate solutions and then potassium thiocyanate solution. The percentage reduction in MEQ fluorescence during 60 s in the presence of potassium thiocyanate was measured and used as an index of VSCC activity. 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), a well characterized chloride channel blocker, and propofol were dissolved in hypotonic gluconate solution to test their effect on VSCC activity. The reduction in fluorescence was inversely related to osmolality, indicating that activation of VSCC is osmolality dependent. Hypotonic gluconate solution (210 mOsm/kg H(2)O) reduced fluorescence by 38.9% +/- 2.6% of the baseline value. The reduction in fluorescence was dose-dependently inhibited by NPPB. Propofol at 0.3, 1, 3, 10, 30, and 100 micro g/mL significantly inhibited the reduction in fluorescence to 23.6% +/- 4.8%, 19.7% +/- 7.4%, 18.2% +/- 3.5%, 17.6% +/- 5.0%, 15.8% +/- 3.1%, and 10.3% +/- 3.9% of the baseline value, respectively. Our results indicate that propofol inhibits VSCC in a dose-dependent manner in human coronary artery smooth muscle cells.
(キーワード): Anesthetics, Intravenous / Cells, Cultured / Chloride Channels / Coronary Vessels / Fluorescent Dyes / Humans / Microscopy, Fluorescence / Muscle, Smooth, Vascular / Nitrobenzoates / Osmolar Concentration / Propofol
(出版サイトへのリンク): ● Publication site (DOI): 10.1213/01.ANE.0000076060.48760.BD
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12933379; ● Search Scopus @ Elsevier (PMID): 12933379; ● Search Scopus @ Elsevier (DOI): 10.1213/01.ANE.0000076060.48760.BD

(DOI: 10.1213/01.ANE.0000076060.48760.BD, PubMed: 12933379) Takashi Kawano, Shuzo Oshita, Yasuo Tsutsumi, Yoshinobu Tomiyama, Hiroshi Kitahata, Yasuhiro Kuroda, Akira Takahashi and Yutaka Nakaya :
Clinically relevant concentrations of propofol have no effect on adenosine triphosphate-sensitive potassium channels in rat ventricular myocytes.,
Anesthesiology, 96, 6, 1472-1477, 2002.

(要約): Activation of adenosine triphosphate-sensitive potassium (K(ATP)) channels produces cardioprotective effects during ischemia. Because propofol is often used in patients who have coronary artery disease undergoing a wide variety of surgical procedures, it is important to evaluate the direct effects of propofol on K(ATP) channel activities in ventricular myocardium during ischemia. The effects of propofol (0.4-60.1 microg/ml) on both sarcolemmal and mitochondrial K(ATP) channel activities were investigated in single, quiescent rat ventricular myocytes. Membrane currents were recorded using cell-attached and inside-out patch clamp configurations. Flavoprotein fluorescence was measured to evaluate mitochondrial oxidation mediated by mitochondrial K(ATP) channels. In the cell-attached configuration, open probability of K(ATP) channels was reduced by propofol in a concentration-dependent manner (EC(50) = 14.2 microg/ml). In the inside-out configurations, propofol inhibited K(ATP) channel activities without changing the single-channel conductance (EC(50) = 11.4 microg/ml). Propofol reduced mitochondrial oxidation in a concentration-dependent manner with an EC(50) of 14.6 microg/ml. Propofol had no effect on the sarcolemmal K(ATP) channel activities in patch clamp configurations and the mitochondrial flavoprotein fluorescence induced by diazoxide at clinically relevant concentrations (< 2 microm), whereas it significantly inhibited both K(ATP) channel activities at very high, nonclinical concentrations (> 5.6 microg/ml; 31 microm).
(キーワード): アデノシン三リン酸 (adenosine triphosphate) / Anesthetics, Intravenous / Animals / Dose-Response Relationship, Drug / 心臓 (heart) / Heart Ventricles / Male / Mitochondria, Heart / Potassium Channels / Propofol / Rats / Rats, Wistar / Sarcolemma
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200206000-00029
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12170062; ● Summary page in Scopus @ Elsevier: 2-s2.0-0036260435

(DOI: 10.1097/00000542-200206000-00029, PubMed: 12170062, Elsevier: Scopus) Yasuo Tsutsumi, Shuzo Oshita, Takashi Kawano, Hiroshi Kitahata, Yoshinobu Tomiyama, Yasuhiro Kuroda and Yutaka Nakaya :
Lidocaine and mexiletine inhibit mitochondrial oxidation in rat ventricular myocytes.,
Anesthesiology, 95, 3, 766-770, 2001.

(要約): Accumulating evidence suggests that mitochondrial rather than sarcolemmal adenosine triphosphate-sensitive K+ (K(ATP)) channels may have an important role in the protection of myocardium during ischemia. Because both lidocaine and mexiletine are frequently used antiarrhythmic drugs during myocardial ischemia, it is important to investigate whether they affect mitochondrial K(ATP) channel activities. Male Wistar rats were anesthetized with ether. Single, quiescent ventricular myocytes were dispersed enzymatically. The authors measured flavoprotein fluorescence to evaluate mitochondrial redox state. Lidocaine or mexiletine was applied after administration of diazoxide (25 microM), a selective mitochondrial K(ATP) channel opener. The redox signal was normalized to the baseline flavoprotein fluorescence obtained during exposure to 2,4-dinitrophenol, a protonophore that uncouples respiration from ATP synthesis and collapses the mitochondrial potential. Diazoxide-induced oxidation of flavoproteins and the redox changes were inhibited by 5-hydroxydecanoic acid, a selective mitochondrial K(ATP) channel blocker, suggesting that flavoprotein fluorescence can be used as an index of mitochondrial oxidation mediated by mitochondrial K(ATP) channels. Lidocaine (10(-3) to 10 mM) and mexiletine (10(-3) to 10 mM) reduced oxidation of the mitochondrial matrix in a dose-dependent manner with an EC50 of 98+/-63 microM for lidocaine and 107+/-89 microM for mexiletine. Both lidocaine and mexiletine reduced flavoprotein fluorescence induced by diazoxide in rat ventricular myocytes, indicating that these antiarrhythmic drugs may produce impairment of mitochondrial oxidation mediated by mitochondrial K(ATP) channels.
(キーワード): Adenosine Triphosphate / Animals / Anti-Arrhythmia Agents / Calcium / Diazoxide / Heart Ventricles / Lidocaine / Male / Mexiletine / Mitochondria, Heart / Oxygen Consumption / Potassium Channels / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00000542-200109000-00032
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11575552; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034849904

(DOI: 10.1097/00000542-200109000-00032, PubMed: 11575552, Elsevier: Scopus) Yoshinobu Tomiyama, Jr. E. B rian Johnney and Todd M Michael :
Plasma viscosity and cerebral blood flow,
American Journal of Physiology, Heart and Circulatory Physiology, 279, 4, H1949-H1954, 2000.

(要約): We hypothesized that the response of cerebral blood flow (CBF) to changing viscosity would be dependent on "baseline" CBF, with a greater influence of viscosity during high-flow conditions. Plasma viscosity was adjusted to 1.0 or 3.0 cP in rats by exchange transfusion with red blood cells diluted in lactated Ringer solution or with dextran. Cortical CBF was measured by H(2) clearance. Two groups of animals remained normoxic and normocarbic and served as controls. Other groups were made anemic, hypercapnic, or hypoxic to increase CBF. Under baseline conditions before intervention, CBF did not differ between groups and averaged 49.4 +/- 10.2 ml. 100 g(-1). min(-1) (+/-SD). In control animals, changing plasma viscosity to 1. 0 or 3.0 cP resulted in CBF of 55.9 +/- 8.6 and 42.5 +/- 12.7 ml. 100 g(-1). min(-1), respectively (not significant). During hemodilution, hypercapnia, and hypoxia with a plasma viscosity of 1. 0 cP, CBF varied from 98 to 115 ml. 100 g(-1). min(-1). When plasma viscosity was 3.0 cP during hemodilution, hypercapnia, and hypoxia, CBF ranged from 56 to 58 ml. 100 g(-1). min(-1) and was significantly reduced in each case (P < 0.05). These results support the hypothesis that viscosity has a greater role in regulation of CBF when CBF is increased. In addition, because CBF more closely followed changes in plasma viscosity (rather than whole blood viscosity), we believe that plasma viscosity may be the more important factor in controlling CBF.
(キーワード): Anemia / Animals / Blood Flow Velocity / Blood Viscosity / Cerebrovascular Circulation / Hemodilution / Hypercapnia / Hypoxia / Male / Rats / Rats, Sprague-Dawley / Reference Values
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpheart.2000.279.4.H1949
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11009484; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033724821

(DOI: 10.1152/ajpheart.2000.279.4.H1949, PubMed: 11009484, Elsevier: Scopus) Yoshinobu Tomiyama, Jr. E. B rian Johnney and Todd M Michael :
Cerebral blood flow during hemodilution and hypoxia in rats: Role of ATP-sensitive potassium channels,
Stroke, 30, 9, 1942-1948, 1999.

(要約): Hypoxia and hemodilution both reduce arterial oxygen content (CaO(2)) and increase cerebral blood flow (CBF), but the mechanisms by which hemodilution increases CBF are largely unknown. ATP-sensitive potassium (K(ATP)) channels are activated by intravascular hypoxia, and contribute to hypoxia-mediated cerebrovasodilatation. Although CaO(2) can be reduced to equal levels by hypoxia or hemodilution, intravascular PO(2) is reduced only during hypoxia. We therefore tested the hypothesis that K(ATP) channels would be unlikely to contribute to cerebrovasodilatation during hemodilution. Glibenclamide (19.8 microg) or vehicle was injected into the cisterna magna of barbiturate-anesthetized rats. The dose of glibenclamide was chosen to yield an estimated CSF concentration of 10(-4) M. Thirty minutes later, some animals underwent either progressive isovolumic hemodilution or hypoxia (over 30 minutes) to achieve a CaO(2) of approximately 7.5 mL O(2)/dL. Other animals did not undergo hypoxia or hemodilution and served as controls. Six groups of animals were studied: control/vehicle (n=4), control/glibenclamide (n=4), hemodilution/vehicle (n=10), hemodilution/glibenclamide (n=10), hypoxia/vehicle (n=10), and hypoxia/glibenclamide (n=10). CBF was then measured with (3)H-nicotine in the forebrain, cerebellum, and brain stem. In control/vehicle rats, CBF ranged from 72 mL. 100 g(-1). min(-1) in forebrain to 88 mL. 100 g(-1) x min(-1) in the brain stem. Glibenclamide treatment of control animals did not influence CBF in any brain area. Hemodilution increased CBF in all brain areas, with flows ranging from 128 mL. 100 g(-1) x min(-1) in forebrain to 169 mL. 100 g(-1) x min(-1) in the brain stem. Glibenclamide treatment of hemodiluted animals did not affect CBF in any brain area. Hypoxia resulted in a greater CBF than did hemodilution, ranging from 172 mL. 100 g(-1) x min(-1) in forebrain to 259 mL. 100 g(-1) x min(-1) in the brain stem. Glibenclamide treatment of hypoxic animals significantly reduced CBF in all brain areas (P<0.05). Both hypoxia and hemodilution increased CBF. Glibenclamide treatment significantly attenuated the CBF increase during hypoxia but not after hemodilution. This finding supports our hypothesis that K(ATP) channels do not contribute to increasing CBF during hemodilution. Because intravascular PO(2) is normal during hemodilution, this finding supports the hypothesis that intravascular PO(2) is an important regulator of cerebral vascular tone and exerts its effect in part by activation of K(ATP) channels in the cerebral circulation.
(キーワード): アデノシン三リン酸 (adenosine triphosphate) / Animals / Anoxia / Cerebrovascular Circulation / Glyburide / Hemodilution / Male / Potassium Channels / Rats / Rats, Sprague-Dawley
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10471448; ● Search Scopus @ Elsevier (PMID): 10471448

(PubMed: 10471448) Yoshinobu Tomiyama, Kevin Jansen, Johnny Jr E Brian and Michael M Todd :
Hemodilution, cerebral O₂ delivery and cerebral blood flow: a study using hyperbaric oxygenation,
American Journal of Physiology, Heart and Circulatory Physiology, 276, 4, H1190-H1196, 1999.

(要約): Hemodilution reduces blood viscosity and O2 content (CaO2) and increases cerebral blood flow (CBF). Viscosity and CaO2 may contribute to increasing CBF after hemodilution. However, because hematocrit is the major contributor to blood viscosity and CaO2, it has been difficult to assess their relative importance. By varying blood viscosity without changing CaO2, prior investigation in hemodiluted animals has suggested that both factors play roughly equal roles. To further investigate the relationship of hemodilution, blood viscosity, CaO2, and CBF, we took the opposite approach in hemodiluted animals, i.e., we varied CaO2 without changing blood viscosity. Hyperbaric O2 was used to restore CaO2 to normal after hemodilution. Pentobarbital sodium-anesthetized rats underwent isovolumic hemodilution with 6% hetastarch, and forebrain CBF was measured with [3H]nicotine. One group of animals did not undergo hemodilution and served as controls (Con). In the three experimental groups, hematocrit was reduced from 44% to 17-19%. Con and hemodiluted (HDil) groups were ventilated with 40% O2 at 101 kPa (1 atmosphere absolute), which resulted in CaO2 values of 19.7 +/- 1.3 and 8.1 +/- 0.7 (SD) ml O2/dl, respectively. A second group of hemodiluted animals (HBar) was ventilated with 100% O2 at 506 kPa (5 atmospheres absolute) in a hyperbaric chamber, which restored CaO2 to an estimated 18.5 +/- 0.5 ml O2/dl by increasing dissolved O2. A fourth group of hemodiluted animals (HCon) served as hyperbaric controls and were ventilated with 10% O2 at 506 kPa, resulting in CaO2 of 9.1 +/- 0.6 ml O2/dl. CBF was 79 +/- 19 ml. 100 g-1. min-1 in the Con group and significantly increased to 123 +/- 9 ml. 100 g-1. min-1 in the HDil group. When CaO2 was restored to baseline with dissolved O2 in the HBar group, CBF decreased to 104 +/- 20 ml. 100 g-1. min-1. When normoxia was maintained during hyperbaric exposure in the HCon group, CBF was 125 +/- 18 ml. 100 g-1. min-1, a value indistinguishable from that in normobaric HDil animals. Our data demonstrate that the reduction in CaO2 after hemodilution is responsible for 40-60% of the increase in CBF.
(キーワード): Animals / Biological Availability / Blood Viscosity / Cerebrovascular Circulation / Hemodilution / Homeostasis / Hyperbaric Oxygenation / Male / Oxygen / Rats / Rats, Sprague-Dawley
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpheart.1999.276.4.H1190
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10199842; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033109933

(DOI: 10.1152/ajpheart.1999.276.4.H1190, PubMed: 10199842, Elsevier: Scopus)

MISC

研究者総覧に該当データはありませんでした。

総説・解説: 研究者総覧に該当データはありませんでした。
講演・発表: Yako Matsumoto, Michiko Kinoshita, Yoshinobu Tomiyama and Katsuya Tanaka :
Protecting Intraoperative Patients In Time Of Natural DisastersPast Experiences And Lessons Learned: A Scoping Review,
The Anesthesiology Annual Meeting 2023, San Francisco, Oct. 2023. Yoshinobu Tomiyama, Kaori Takata, Katsuya Tanaka and Shuzo Oshita :
The effects of hyperkalemia and ouabain during Ischemia on the membrane potential during ischemia/reperfusion,
The Annual Meeting of the American Society of Anesthesiologists, Oct. 2011. Yoshinobu Tomiyama :
Clinical usefulness of new drug. Sugammadex, for optimal reversal of neuromuscular block,
日中麻酔科学会議, 徳島, Nov. 2010. Yoshinobu Tomiyama, Kaori Takata, Katsuya Tanaka and Shuzo Oshita :
The Effects of Hyperkalemia and Ouabain on Membrane Integrity during Ischemia/Reperfusion.,
American Society of Anesthesiologists 2010 Annual Meeting, Oct. 2010. Kaori Takata, Yoshinobu Tomiyama, Katsuya Tanaka, Hiroshi Kitahata and Shuzo Oshita :
Role of hyperkalemia and Na+/K+ ATPase in myocardial protection during simulated ischemia.,
The Annual Meeting of the American Society of Anesthesiologists, New Orleans, Oct. 2009. Hiroshi Kitahata, Junpei Nozaki, Shinji Kawahito, Yoshinobu Tomiyama and Shuzo Oshita :
Interaction of the β1-blocker landiolol with early and late sevoflurane-induced preconditioning.,
The Annual Meeting of the American Society of Anesthesiologists, New Orleans, Oct. 2009. Nakamura Tomoka, Shinji Kawahito, Hiroshi Kitahata, Yoshinobu Tomiyama and Shuzo Oshita :
Intensive insulin therapy in patients undergoing living-related liver transplantation.,
The Annual Meeting of the American Society of Anesthesiologists, Orlando, Oct. 2008. Shinji Kawahito, Akio Iseki, Yoshinobu Tomiyama, Hiroshi Kitahata and Shuzo Oshita :
High-frequency jet ventilation during thoracoscopic sympathectomy for palmar hyperhidrosis.,
The Annual Meeting of the American Society of Anesthesiologists, San Francisco, Oct. 2007. Hiroshi Kitahata, Junpei Nozaki, Shinji Kawahito, Yoshinobu Tomiyama and Shuzo Oshita :
The involvement of the mitochondrial KATP channel on heat-shock protein-induced cardioprotection.,
The Annual Meeting of the American Society of Anesthesiologists, Chicago, Oct. 2006. Yoshinobu Tomiyama, Yamaguchi Mikiyo, Satoru Eguchi, Hiroshi Kitahata and Shuzo Oshita :
Effects of propofol on the changes in membrane potential induced by simulated ischemia/reperfusion by means of mitochondrial uncoupler in cultured human coronary artery endothelial cells.,
The Annual Meeting of the American Society of Anesthesiologists, Atlanta, Oct. 2005. Hiroshi Kitahata, Junpei Nozaki, Shinji Kawahito, Yoshinobu Tomiyama and Shuzo Oshita :
Influences of sevoflurane in myocardial protection of an ultra-short-acting β1-blocker, landiolol.,
The Annual Meeting of the American Society of Anesthesiologists, Atlanta, Oct. 2005. Yoshinobu Tomiyama, Tosiko Katayama, Yamaguchi Mikiyo, Hiroshi Kitahata and Shuzo Oshita :
Involvement of Na+/K+ ATPase in the response of membrane potential to mannitol-induced hyperosmolality in cultured human coronary endothelial cells.,
The Annual Meeting of the American Society of Anesthesiologists, Las Vegas, Oct. 2004. Takashi Kawano, Yasuo Tsutsumi, Shuzo Oshita, Yoshinobu Tomiyama and Hiroshi Kitahata :
Effects of propofol and fentanyl on ATP-sensitive potassium channels in cloned pancreatic β-cells.,
The Annual Meeting of the American Society of Anesthesiologists, A-51, Orlando, Oct. 2002. Takashi Kawano, Yasuo Tsutsumi, Shuzo Oshita, Yoshinobu Tomiyama and Hiroshi Kitahata :
Effects of propofol on cloned ATP-sensitive potassium channels in ventricular myocytes are different from those in vascular smooth muscle cells.,
The Annual Meeting of the American Society of Anesthesiologists, A-50, Orlando, Oct. 2002. Yoshinobu Tomiyama, Takako Masuda, Hiroshi Kitahata, Yasuhiro Kuroda and Shuzo Oshita :
Propofol inhibits volume-sensitive chloride channels in human coronary artery smooth muscle cells.,
The Annual Meeting of the American Society of Anesthesiologists, A-629, New Orleans, Oct. 2001. Yoshinobu Tomiyama, Takako Masuda, Hiroshi Kitahata, Yasuhiro Kuroda and Shuzo Oshita :
Volume-sensitive chloride channels in human coronary artery smooth muscle cells.,
The Annual Meeting of the American Society of Anesthesiologists, A-630, Orlando, Oct. 2001. Hideyuki Kimura, Yoshiaki Hirose, Katsuya Tanaka, Shinji Kawahito, Yoshinobu Tomiyama, Hiroshi Kitahata and Shuzo Oshita :
Nitric oxide dose not play a major role in regulation of systemic hemodynamic response to acute normovolemic hemodilution.,
The Annual Meeting of the American Society of Anesthesiologists, San Diego, Oct. 1997. Yoshinobu Tomiyama, Satoshi Yasumoto, Hiroshi Kitahata, Hideyuki Kimura and Shuzo Oshita :
Coagulation status of the whole blood preoperatively obtained in the operating room and preserved at room temperature: Thrombelastographic evaluation.,
The Annual Meeting of the American Society of Anesthesiologists, New Orleans, Oct. 1996. 富山芳信, 仁井内浩, 高松千洋, 松永明 :
体内遺残防止についてのアンケート調査2019,
日本手術医学会誌, 41, Supplement, 124, 2020年12月. 富山芳信, 川西良典, 笠井飛鳥 :
腹腔鏡胸腔鏡手術時間の年次推移,
日本手術医学会誌, 41, Supplement, 164, 2019年9月. 富山芳信, 川西良典, 笠井飛鳥, 石川雄樹, 板東悠太郎 :
手術終了ー退室時間に影響及ぼす諸因子の検討,
麻酔, 67, 2019年5月. 山田将之, 四宮加容, 仁木昌徳, 富山芳信, 湯浅真由, 三田村佳典 :
眼科手術中にラテックスアレルギーよるアナフィラキシーショックをきたした一例,
第72回日本臨床眼科学会(2018/10/10∼14), 2018年10月. 富山芳信, 川西良典, 箕田直治 :
予定手術時間と実施手術時間の差に及ぼす諸因子の検討,
日本手術医学会誌, 39, supplement, 116, 2018年9月. 富山芳信, 川西良典, 箕田直治 :
術後X線単純撮影とワイヤレスFDP搭載撮影装置が手術終了ー退室時間に及ぼす影響,
日本手術医学会誌, 40, Supplement, 116, 2018年9月. 箕田直治, 坂東悠太郎, 里美志帆, 曽我朋宏, 富山芳信, 木下浩之, 川人伸次, 田中克哉, 高石和美, 北畑洋 :
脱血管挿入部での縫着のため肺動脈カテーテル抜去困難となり開胸下抜去術が必要になった症例,
第39回日本循環制御医学会総会, 2018年6月. 高石和美, 川人伸次, 原田永勝, 富山芳信, 北畑洋 :
新生児ラット心筋細胞におけるβ3受容体刺激が一酸化窒素産生に及ぼす影響,
日本麻酔科学会第60回学術集会, 2013年5月. 角田奈美, 門田尚子, 田中克哉, 堤保夫, 曽我朋宏, 髙田香, 富山芳信, 大下修造 :
食道癌により食道気管廔を形成した患者に右用ダブルルーメンチューブを用いて麻酔管理をした一症例,
日本臨床麻酔学会第31回大会, 2011年11月. 富山芳信 :
手術室運用の改善と解決すべき課題,
日本麻酔科学会中国・四国支部学術集会, 2011年9月. 富山芳信, 野村佳世, 角田奈美, 大下修造 :
重症筋無力症に，ロクロニウムとスガマデクスを使用した症例,
日本臨床麻酔学会第30回大会, 2010年11月. 江口覚, 山下菊治, 石川友康, 富山芳信, 大下修弘, 細川浩良, 北村清一郎, 中條信義 :
遠赤外線照射は麻酔効果を抑制する,
第22回麻酔メカニズム研究会, 2004年7月.

研究会・報告書: 山田将之, 四宮加容, 大串陽子, 富山芳信, 鉄谷真由, 三田村佳典 :
全身麻酔手術中にラテックスアレルギーによるアナフィラキシーショックをきたした1例,
第79回徳島眼科集談会(2017/12/10), 2017年12月.

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 心筋虚血時に細胞外に蓄積するKイオンは、細胞内Caイオンの上昇を抑制するか (研究課題/領域番号: 19591801 )
生体吸収性ポリマーを用いた人工血管の開発 (研究課題/領域番号: 16592004 )
脳血流の自己調節機構(AR)を非侵襲的に評価できる数理モデルの開発 (研究課題/領域番号: 15659428 )
心保護効果における細胞膜とミトコンドリアのATP感受性Kチャネルの相互作用 (研究課題/領域番号: 15591636 )
容量感受性クロライドチャネル活性化に及ぼす麻酔薬の影響 (研究課題/領域番号: 12671476 )
希釈式自己血輸血における代用血漿投与が血液凝固線溶系および腎機能に及ぼす影響 (研究課題/領域番号: 08771196 )
虚血時および再灌流時の心筋細胞内カルシウム濃度変化に及ぼす灌流温低下の効果 (研究課題/領域番号: 07671665 )
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2026年4月21日更新

専門分野・研究分野: 麻酔科学 (Anesthesiology)
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Jグローバル最終確認日: 2026/4/18 01:35
氏名（漢字）: 富山芳信
氏名（フリガナ）: トミヤマヨシノブ
氏名（英字）: Tomiyama Yoshinobu
所属機関: 徳島大学准教授

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researchmap最終確認日: 2026/4/19 03:11
氏名（漢字）: 富山芳信
氏名（フリガナ）: トミヤマヨシノブ
氏名（英字）: Tomiyama Yoshinobu
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登録日時: 2011/2/1 00:00
更新日時: 2020/8/29 13:39
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所属ID: 0344003000
所属: 徳島大学
部署: 徳島大学病院手術部
職名: 准教授
学位: 博士(医学)
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研究者番号: 30243702

所属（現在）: 2026/4/1 : 徳島大学, 病院, 准教授

所属（過去の研究課題 情報に基づく）*注記: 2007/4/1 – 2008/4/1 : 徳島大学, 医学部・歯学部附属病院, 准教授
2005/4/1 : 徳島大学, 医学部・歯学部附属病院, 助教授
2004/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 講師
2003/4/1 – 2004/4/1 : 徳島大学, 医学部, 講師
2001/4/1 : 徳島大学, 医学部, 講師
2000/4/1 : 徳島大学, 医学部・附属病院, 講師
1995/4/1 – 1996/4/1 : 徳島大学, 医学部・附属病院, 助手

審査区分/研究分野

研究代表者

医学 / 外科 / 麻酔・蘇生学

研究代表者以外

医学 / 外科 / 麻酔・蘇生学
生物系 / 医歯薬学 / 外科系臨床医学 / 救急医学
生物系 / 医歯薬学 / 外科系臨床医学 / 麻酔・蘇生学
生物系 / 医歯薬学 / 歯学 / 外科系歯学

キーワード

研究代表者

希釈式自己血輸血 / トロンボエラストグラフィー / Dダイマー / クレアチニンクリアランス / N-Acethyl-beta-d-Glucosaminidase / ハイドロキシエナルスターチ / 容量感受性クロライドチャネル / プロポフォール / 冠状動脈平滑筋細胞 / 臓器保護 / 臓器毒性 / 麻酔薬 / 冠動脈平滑筋細胞 / Volume-sensitive chloride channel / propofol / coronary artery smooth muscle cell / organ protection / organ toxicity / anesthetics

研究代表者以外

心室筋 / 虚血 / カルシウム / フラ-2 / lysophosphatidylcholine / 低温 / 経頭蓋脳血流ドップラ / 脳血流の自己調節 / 重症脳障害 / 経頭蓋脳血流ドプラ / 脳血流の自己調整 / 心筋虚血 / 細胞外カリウムイオン濃度 / 細胞内カルシウムイオン濃度 / Na^+-K^+ATPase / 高カリウム血症 / 細胞内カルシウム / Na-Kポンプ活性 / 循環器・高血圧 / 電解質 / カリウム / 先行虚血 / 心保護効果 / パッチクランプ法 / カリウムチャネル / ミトコンドリア / 血管平滑筋細胞 / 麻酔薬 / イソフルラン / 心筋保護効果 / Kチャネル / Ischemic Preconditioning / Cardioprotection / Patchclamp configuration / Potassium channel / Mitochondria / Vascular smooth muscle cells / Anesthetics / Isoflurane / 細胞培養 / コラーゲン / 内皮細胞 / 血球成分 / cell culture / collagen / blood cells

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