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佐藤陽一

徳島大学

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2024年11月25日更新

職名: 教授
電話: 088-633-7253
電子メール: youichi.sato@tokushima-u.ac.jp
学歴: 1992/3: 徳島大学薬学部薬学科卒業
学位: 博士(薬学) (徳島大学) (2004年3月)
職歴・経歴: 2003/7: 徳島大学大学院ヘルスバイオサイエンス研究部助手
2010/5: 徳島大学大学院ヘルスバイオサイエンス研究部准教授
2020/10: 徳島大学大学院医歯薬学研究部教授

専門分野・研究分野: 人類遺伝学 (Human Genetics)

研究者総覧で最新データを確認する

2024年11月25日更新

専門分野・研究分野: 人類遺伝学 (Human Genetics)
担当経験のある授業科目: ゲノム創薬特論 (大学院)
先端医療薬学 (学部)
先端臨床研究入門 (学部)
医療薬学実践演習 (大学院)
医薬品安全性学特論 (大学院)
医薬品情報学 (学部)
医薬品情報学1 (学部)
医薬品情報学2 (学部)
医薬品情報解析学 (学部)
学術論文作成法 (学部)
実務実習事前学習 (学部)
実践医薬品情報学特論 (大学院)
専攻公開ゼミナール (大学院)
演習 (学部)
演習1 (学部)
演習3 (学部)
生命科学の研究手法 (大学院)
生物化学実習 (学部)
社会医学・疫学・医学統計概論 (大学院)
社会医学・疫学・医学統計概論 (Introduction to social medicine,epidemiology and biostatistics) (大学院)
育薬共通演習 (大学院)
薬剤動態制御学特論 (大学院)
薬学演習 (大学院)
薬学科学特論Ⅰ (大学院)
薬学課題研究 (大学院)
薬科学演習1 (大学院)
薬科学特別研究 (大学院)
衛生薬学2(疾病) (学部)
遺伝子生化学 (学部)
指導経験: 研究者総覧に該当データはありませんでした。

研究者総覧で最新データを確認する

2024年11月25日更新

専門分野・研究分野: 人類遺伝学 (Human Genetics)

研究テーマ: 疾患感受性及び薬剤応答性関連遺伝子の探索と機能解析, 医薬品適正使用に向けた疫学的研究 (Y染色体 (Y chromosome), 男性不妊症, ファーマコゲノミクス)

著書

佐藤陽一 :
図解医薬品情報学改訂4版 (折井孝男編集)「第4章2 製薬企業が市販後に提供・構築する情報」,
株式会社南山堂, 2019年10月. 桐野豊, 土屋浩一郎, 佐藤陽一, 阿部真治, 佐藤智恵美 :
世界薬学探訪記四国の全薬学部による海外薬学視察団最新報告: 日本の薬学，薬剤師はどう変わるべきか?,
2019年4月.

論文

Yuki Kumihashi, Yohei Kasai, Takuya Akagawa, Yasuhiro Yuasa, Hisashi Ishikura and Youichi Sato :
Study on prediction of early adverse events by CapeOX therapy in patients with colorectal cancer,
The Journal of Medical Investigation : JMI, Vol.71, No.1,2, 141-147, 2024.

(徳島大学機関リポジトリ): ● Metadata: 119466
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.71.141
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.71.141

(徳島大学機関リポジトリ: 119466, DOI: 10.2152/jmi.71.141) Youichi Sato :
Y chromosome haplogroups are associated with birth size in Japanese men,
The Journal of Medical Investigation : JMI, Vol.71, No.1,2, 129-133, 2024.

(徳島大学機関リポジトリ): ● Metadata: 119462
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.71.129
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.71.129

(徳島大学機関リポジトリ: 119462, DOI: 10.2152/jmi.71.129) Makoto Inoue and Youichi Sato :
An update and frequency distribution of Y chromosome haplogroups in modern Japanese males,
Journal of Human Genetics, Vol.69, No.3, 107-114, 2024.

(要約): Japanese males belong to the Y chromosome C1a1, C2, D1a2a, D1a2a-12f2b, O1b2, O1b2a1a1, O2a2b1, and O2a1b haplogroups. Notably, the regional frequency of each haplogroup is homogeneous. Owing to recent developments in genome sequencing technology, the phylogenetic tree of Y chromosome haplogroups is updated annually. Therefore, in this study, we aimed to provide an update on the Y chromosome haplogroups of modern Japanese males and examine their regional distributions. Using 1,640 samples of Japanese males from seven Japanese cities (Nagasaki, Fukuoka, Tokushima, Osaka, Kanazawa, Kawasaki, and Sapporo), haplogroups C1a1, C2, D1a2a, D1a2a-12f2b, O1b2, and O1b2a1a1 were updated based on the latest phylogenetic tree. Haplogroup C1a1 was mainly classified into C1a1a1a and C1a1a1b subgroups; C1a1a1b was more common in Tokushima and Osaka than in the other regions. Haplogroup C2 was mainly classified into C2a, C2b1a1a, C2b1a1b, C2b1a2, and C2b1b subgroups and exhibited frequency differences in Osaka. Haplogroup D1a2a was classified into D1a2a1c1 and D1a2a2 subgroups, and its frequency varied between Tokushima and Osaka. Haplogroup D1a2a-12f2b was classified into D1a2a1a2b1a1a and D1a2a1a3 subgroups; however, no significant frequency differences were observed. Haplogroup O1b2 was classified into O1b2a1a2a1a, O1b2a1a2a1b, and O1b2a1a3 subgroups, with frequency differences between Nagasaki and Kanazawa. Haplogroup O1b2a1a1 was mainly classified into O1b2a1a1a, O1b2a1a1b, and O1b2a1a1c subgroups; however, no significant frequency differences were observed. Our findings suggest that gene flow in the Kinki region is caused by human migration.
(キーワード): Male / Humans / Japan / Phylogeny / Haplotypes / Chromosomes, Human, Y / Chromosome Mapping
(徳島大学機関リポジトリ): ● Metadata: 119143
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s10038-023-01214-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38123667; ● Search Scopus @ Elsevier (PMID): 38123667; ● Search Scopus @ Elsevier (DOI): 10.1038/s10038-023-01214-5

(徳島大学機関リポジトリ: 119143, DOI: 10.1038/s10038-023-01214-5, PubMed: 38123667) Yusuke Nakagawa, Atsushi Tada, Kosuke Kojo, Haruki Tsuchiya, Masahiro Kurobe, Masahiro Uchida, Kazumitsu Yamasaki, Teruaki Iwamoto and Youichi Sato :
Analysis of the correlation between gene copy deletion in the AZFc region and male infertility in Japanese men,
Reproductive Biology, Vol.23, No.1, 100728, 2023.

(要約): Deletion of the azoospermia factor c (AZFc), located on the long arm of the Y chromosome, is a cause of male infertility. The structure of the Y chromosome is diversified by the copy number of various genes, such as deleted in azoospermia (DAZ), basic protein Y2, chromodomain Y1, testis-specific transcript Y-linked 4, and Golgi autoantigen golgin subfamily a2 like Y, located in the AZF region. In this study, we investigated the deletion of each gene copy and analyzed its relationship with Japanese male infertility. Deletions of single nucleotide variants of each gene copy in 721 proven fertile men as controls, 139 patients with non-obstructive azoospermia (NOA), and 56 patients with oligozoospermia (OS) were analyzed via polymerase chain reaction-restriction fragment length polymorphism analysis. Their association with infertility was analyzed using logistic regression analysis adjusted for the Y-chromosome haplogroup, D1a2a. Deletions of DAZ/II in the r1 region and DAZ/V in the r1 and r2 regions showed significant associations with NOA (odds ratio [OR] = 4.15, 95 % confidence interval [CI] = 1.18-14.6, P = 0.026; OR = 4.19, 95 % CI = 1.19-14.7, P = 0.025, respectively). They did not show any association with OS. Partial deletion of the AZFc region affects spermatogenesis in Japanese male.
(徳島大学機関リポジトリ): ● Metadata: 117924
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.repbio.2022.100728
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36640629; ● Search Scopus @ Elsevier (PMID): 36640629; ● Search Scopus @ Elsevier (DOI): 10.1016/j.repbio.2022.100728

(徳島大学機関リポジトリ: 117924, DOI: 10.1016/j.repbio.2022.100728, PubMed: 36640629) Mayu Fukutomi, Chiharu Uedono, Aki Fujii and Youichi Sato :
Lrriq1 is an essential factor for fertility by suppressing apoptosis.,
Journal of Assisted Reproduction and Genetics, Vol.39, No.11, 2647-2657, 2022.

(要約): Leucine-rich repeats and IQ motif containing 1 (LRRIQ1) gene is reportedly associated with plasma inhibin B levels. However, the function of LRRIQ1 remains unknown. In this study, we generated Lrriq1 knockout mice (Lrriq1 mice) and examined the effects of LRRIQ1 on inhibin B and fertility. Lrriq1 mice were generated using CRISPR/Cas9 genome editing technology. The expression of Inhibin B was examined by Western blotting using a protein extracted from the testis of a 3-month-old male mouse. Mating experiments were conducted using 7-week-old Lrriq1 mice and wild-type (WT) mice to examine fertility. Sperm concentration and sperm motility were measured using 3-month-old male mice. Expression analysis of inhibin B revealed that Lrriq1 mice exhibited reduced mRNA and protein levels of inhibin alpha (Inha), which constitutes the α subunit. In the mating experiment, the litter size of Lrriq1 male mice was 4.3 ± 2.9, which was significantly lower than that of WT male mice (8.3 ± 1.3) (p < 0.001). No difference in sperm count was observed between Lrriq1 and WT male mice; however, sperm motility (%) was significantly reduced in Lrriq1 mice (48.4 ± 4.9) when compared with WT mice (70.2 ± 4.7) (p < 0.001). Based on TUNEL staining, the testes and epididymal sperm of Lrriq1 mice showed high numbers of apoptosis-positive cells. Lrriq1 knockout reduced sperm motility and litter size by inducing apoptosis of testicular germ cells and epididymal sperm.
(キーワード): Male / Mice / Animals / Sperm Motility / Semen / Fertility / Sperm Count / Testis / Spermatozoa / Mice, Knockout / Apoptosis
(徳島大学機関リポジトリ): ● Metadata: 117586
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10815-022-02623-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36129630; ● Search Scopus @ Elsevier (PMID): 36129630; ● Search Scopus @ Elsevier (DOI): 10.1007/s10815-022-02623-9

(徳島大学機関リポジトリ: 117586, DOI: 10.1007/s10815-022-02623-9, PubMed: 36129630) Hiroki Yamada, Rio Ohmori, Naoto Okada, Shingen Nakamura, Kumiko Kagawa, Shiroh Fujii, Hirokazu Miki, Keisuke Ishizawa, Masahiro Abe and Youichi Sato :
A machine learning model using SNPs obtained from a genome-wide association study predicts the onset of vincristine-induced peripheral neuropathy,
The Pharmacogenomics Journal, 2022.

(要約): Vincristine treatment may cause peripheral neuropathy. In this study, we identified the genes associated with the development of peripheral neuropathy due to vincristine therapy using a genome-wide association study (GWAS) and constructed a predictive model for the development of peripheral neuropathy using genetic information-based machine learning. The study included 72 patients admitted to the Department of Hematology, Tokushima University Hospital, who received vincristine. Of these, 56 were genotyped using the Illumina Asian Screening Array-24 Kit, and a GWAS for the onset of peripheral neuropathy caused by vincristine was conducted. Using Sanger sequencing for 16 validation samples, the top three single nucleotide polymorphisms (SNPs) associated with the onset of peripheral neuropathy were determined. Machine learning was performed using the statistical software R package "caret". The 56 GWAS and 16 validation samples were used as the training and test sets, respectively. Predictive models were constructed using random forest, support vector machine, naive Bayes, and neural network algorithms. According to the GWAS, rs2110179, rs7126100, and rs2076549 were associated with the development of peripheral neuropathy on vincristine administration. Machine learning was performed using these three SNPs to construct a prediction model. A high accuracy of 93.8% was obtained with the support vector machine and neural network using rs2110179 and rs2076549. Thus, peripheral neuropathy development due to vincristine therapy can be effectively predicted by a machine learning prediction model using SNPs associated with it.
(徳島大学機関リポジトリ): ● Metadata: 117583
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41397-022-00282-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35752658; ● Search Scopus @ Elsevier (PMID): 35752658; ● Search Scopus @ Elsevier (DOI): 10.1038/s41397-022-00282-8

(徳島大学機関リポジトリ: 117583, DOI: 10.1038/s41397-022-00282-8, PubMed: 35752658) Minori Takei, Naoto Okada, Shingen Nakamura, Kumiko Kagawa, Shiroh Fujii, Hirokazu Miki, Keisuke Ishizawa, Masahiro Abe and Youichi Sato :
A genome-wide association study predicts the onset of dysgeusia due to anti-cancer drug treatment,
Biological & Pharmaceutical Bulletin, Vol.45, No.1, 114-117, 2022.

(要約): Dysgeusia is a major side effect of anti-cancer drug treatment. Since dysgeusia significantly lowers the patient's QOL, predicting and avoiding its onset in advance is desirable. Accordingly, aims of the present study were to use a genome-wide association study (GWAS) to identify genes associated with the development of dysgeusia in patients taking anti-cancer drugs and to predict the development of dysgeusia using associated single nucleotide polymorphisms (SNPs). GWAS was conducted on 76 patients admitted to the Department of Hematology, Tokushima University Hospital. Using Sanger sequencing for 23 separately collected validation samples, the top two SNPs associated with the development of dysgeusia were determined. GWAS identified rs73049478 and rs41396146 SNPs on the retinoic acid receptor beta (RARB) gene associated with dysgeusia development due to the administration of anti-cancer drugs. Evaluation of the two SNPs using 23 validation samples indicated that the accuracy rate of rs73049478 was relatively high (87.0%). Thus, the findings of the present study suggest that the rs73049478 SNP of RARB can be used to predict the onset of dysgeusia caused by the administration of anti-cancer drugs.
(キーワード): Antineoplastic Agents / Dysgeusia / Genetic Predisposition to Disease / Genome-Wide Association Study / Humans / Pharmaceutical Preparations / Polymorphism, Single Nucleotide / Quality of Life
(徳島大学機関リポジトリ): ● Metadata: 117584
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.b21-00745
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34657909; ● CiNii @ 国立情報学研究所 (CRID): 1390290617368322560; ● Summary page in Scopus @ Elsevier: 2-s2.0-85123201593

(徳島大学機関リポジトリ: 117584, DOI: 10.1248/bpb.b21-00745, PubMed: 34657909, CiNii: 1390290617368322560, Elsevier: Scopus) Tashima Hozumi, Endo Yuka, Naoto Okada, Shingen Nakamura, Kumiko Kagawa, Shiroh Fujii, Hirokazu Miki, Keisuke Ishizawa, Masahiro Abe and Youichi Sato :
Association analysis between adverse drug reactions to cytarabine therapy and single nucleotide polymorphisms in cytarabine metabolic genes in patients with hematopoietic tumor,
Personalized Medicine Universe, Vol.10, 1-6, 2021.

(徳島大学機関リポジトリ): ● Metadata: 117585
(出版サイトへのリンク): ● Publication site (DOI): 10.46459/pmu.2021002
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.46459/pmu.2021002

(徳島大学機関リポジトリ: 117585, DOI: 10.46459/pmu.2021002) Youichi Sato, Atsushi Tajima, Misaki Kiguchi, Suzu Kogusuri, Aki Fujii, Takehiro Sato, Shiari Nozawa, Miki Yoshiike, Makiko Mieno, Kosuke Kojo, Masahiro Uchida, Haruki Tsuchiya, Kazumitu Yamasaki, Issei Imoto and Teruaki Iwamoto :
Genome-wide association study of semen volume, sperm concentration, testis size, and plasma inhibin B levels,
Journal of Human Genetics, Vol.65, No.8, 683-691, 2020.

(要約): Semen quality is affected by environmental factors, endocrine function abnormalities, and genetic factors. A GWAS recently identified ERBB4 at 2q34 as a genetic locus associated with sperm motility. However, GWASs for human semen volume and sperm concentration have not been conducted. In addition, testis size also reportedly correlates with semen quality, and it is important to identify genes that affect testis size. Reproductive hormones also play an important role in spermatogenesis. To date, genetic loci associated with plasma testosterone, sex hormone-binding globulin (SHBG), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels have been identified using GWASs. However, GWASs have not identified any relevant loci for plasma inhibin B levels. We conducted a two-stage GWAS using 811 Japanese men in a discovery stage followed by a replication stage using an additional 721 Japanese men. The results of the discovery and replication stages were combined into a meta-analysis. After setting a suggestive significance threshold for P values < 5 × 10 in the discovery stage, we identified ten regions with SNPs (semen volume: one, sperm concentration: three, testes size: two, and inhibin B: four). We selected only the most significant SNP in each region for replication genotyping. Combined discovery and replication results in the meta-analysis showed that the locus 12q21.31 associated with plasma inhibin B levels (rs11116724) had the most significant association (P = 5.7 × 10). The LRRIQ1 and TSPAN19 genes are located in the 12q21.31 region. This study provides new susceptibility variants that contribute to plasma inhibin B levels.
(徳島大学機関リポジトリ): ● Metadata: 115234
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s10038-020-0757-3
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32341457; ● Summary page in Scopus @ Elsevier: 2-s2.0-85084145101

(徳島大学機関リポジトリ: 115234, DOI: 10.1038/s10038-020-0757-3, PubMed: 32341457, Elsevier: Scopus) Tomoko Otani, Yasuko Kase, Kazufumi Kunitomo, Kazumi Shimooka, Mitsugu Naoe, Hiroko Yamamoto, Kazuyoshi Kawazoe, Youichi Sato and Aiko Yamauchi :
Novel formula using triceps skinfold thickness to revise the Cockcroft-Gault equation for estimating renal function in Japanese bedridden elderly patients,
The Journal of Medical Investigation : JMI, Vol.65, No.3-4, 195-202, 2018.

(要約): In recumbent elderly patients, creatinine clearance (eCCr) estimated by the Cockcroft-Gault (CG) equation may not necessarily reflect renal function. We aimed to develop a novel formula to revise the CG equation using anthropometric measurements in bedridden elderly patients and evaluate its clinical utility. The subjects included 77 bedridden Japanese patients aged ≦ 65, hospitalized at Naruto Yamakami Hospital. The actual CCr (mCCr) value was measured using the 24-hour urine collection method. Anthropometric data, such as skeletal muscle mass, body fat mass (BFM), and triceps skinfold thickness (TSF), were collected. We established a novel formula to estimate CCr or CCr by correcting the eCCr value with BFM or TSF. The stage of classification of renal dysfunctions in patients with eGFR or eGFR was equivalent to the GFR based on the mCCr. Notably, the novel equation for eCCr based on TSF (eCCr), dubbed the "Naruto" formula, can be useful to evaluate renal function in bedridden elderly patients without expensive equipment or additional costs. In this study, mCCr was considered to be the true renal function of the patient, but whether and to what extent mCCr correlates with inulin clearance is unknown. J. Med. Invest. 65:195-202, August, 2018.
(徳島大学機関リポジトリ): ● Metadata: 112237
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.65.195
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30282860; ● Summary page in Scopus @ Elsevier: 2-s2.0-85054455235

(徳島大学機関リポジトリ: 112237, DOI: 10.2152/jmi.65.195, PubMed: 30282860, Elsevier: Scopus) Tomoko Otani, Yasuko Kase, Kazufumi Kunitomo, Kazumi Shimooka, Mitsugu Naoe, Hiroko Yamamoto, Kazuyoshi Kawazoe, Youichi Sato and Aiko Yamauchi :
What is the correct adjustment protocol for serum creatinine value to reflect renal function in bedridden elderly patients?,
The Japanese Journal of Nephrology and Pharmacotherapy, Vol.7, No.1, 3-12, 2018.

(出版サイトへのリンク): ● Publication site (DOI): 10.24595/jjnp.7.1_3
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.24595/jjnp.7.1_3

(DOI: 10.24595/jjnp.7.1_3) Youichi Sato, Atsushi Tajima, Takehiro Sato, Shiari Nozawa, Miki Yoshiike, Issei Imoto, Aiko Yamauchi and Teruaki Iwamoto :
Genome-wide association study identifies ERBB4 on 2q34 as a novel locus associated with sperm motility in Japanese men.,
Journal of Medical Genetics, Vol.55, No.6, 415-421, 2018.

(要約): The decrease in sperm motility has a potent influence on fertilisation. Sperm motility, represented as the percentage of motile sperm in ejaculated sperms, is influenced by lifestyle habits or environmental factors and by inherited factors. However, genetic factors contributing to individual differences in sperm motility remain unclear. To identify genetic factors that influence human sperm motility, we performed a genome-wide association study (GWAS) of sperm motility. A two-stage GWAS was conducted using 811 Japanese men in a discovery stage, followed by a replication study using an additional 779 Japanese men. In the two-staged GWAS, a single nucleotide polymorphism rs3791686 in the intron of gene for erb-b2 receptor tyrosine kinase 4 () on chromosome 2q34 was identified as a novel locus for sperm motility, as evident from the discovery and replication results using meta-analysis (β=-4.01, combined P=5.40×10). Together with the previous evidence that Sertoli cell-specific -knockout mice display an impaired ability to produce motile sperm, this finding provides the first genetic evidence for further investigation of the genome-wide significant association at the locus in larger studies across diverse human populations.
(徳島大学機関リポジトリ): ● Metadata: 113933
(出版サイトへのリンク): ● Publication site (DOI): 10.1136/jmedgenet-2017-104991
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29453196; ● Search Scopus @ Elsevier (PMID): 29453196; ● Search Scopus @ Elsevier (DOI): 10.1136/jmedgenet-2017-104991

(徳島大学機関リポジトリ: 113933, DOI: 10.1136/jmedgenet-2017-104991, PubMed: 29453196) Youichi Sato, Chise Hasegawa, Atsushi Tajima, Shiari Nozawa, Miki Yoshiike, Eitetsue Koh, Jiro Kanaya, Mikio Namiki, Kiyomi Matsumiya, Akira Tsujimura, Kiyoshi Komatsu, Naoki Itoh, Jiro Eguchi, Aiko Yamauchi and Teruaki Iwamoto :
Association of TUSC1 and DPF3 gene polymorphisms with male infertility,
Journal of Assisted Reproduction and Genetics, Vol.35, No.2, 257-263, 2018.

(要約): Recently, genome-wide association studies of a Hutterite population in the USA revealed that five single nucleotide polymorphisms (SNPs) with a significant association with sperm quality and/or function in ethnically diverse men from Chicago were significantly correlated with family size. Of these, three SNPs (rs7867029, rs7174015, and rs12870438) were found to be significantly associated with the risk of azoospermia and/or oligozoospermia in a Japanese population. In this study, we investigated whether the rs10966811 (located in an intergenic region between the TUSC1 and IZUMO3 genes) and rs10129954 (located in the DPF3 gene) SNPs, previously related to family size, are associated with male infertility. In addition, we performed association analysis between rs12348 in TUSC1 and rs2772579 in IZUMO3 and male infertility. We genotyped 145 patients with infertility (including 83 patients with azoospermia and 62 with oligozoospermia) and 713 fertile controls by PCR-RFLP technique for polymorphism. Because rs10966811 has no restriction sites, the SNP rs12376894 with strong linkage disequilibrium was selected as an alternative to rs10966811. There was a statistically significant association between rs12376894 proxy SNP of rs10966811 and oligozoospermia. Also, a statistically significant association between rs10129954 and azoospermia, and oligozoospermia was observed. When we assessed the relationship between rs12348 in TUSC1 and rs2772579 in IZUMO3 and male infertility traits, we found that rs12348 in TUSC1 was significantly associated with azoospermia and oligozoospermia, but rs2772579 in IZUMO3 was not associated with male infertility. We found that the polymorphisms in TUSC1 and DPF3 displayed strong associations with male infertility.
(徳島大学機関リポジトリ): ● Metadata: 113935
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10815-017-1052-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28975488; ● Search Scopus @ Elsevier (PMID): 28975488; ● Search Scopus @ Elsevier (DOI): 10.1007/s10815-017-1052-x

(徳島大学機関リポジトリ: 113935, DOI: 10.1007/s10815-017-1052-x, PubMed: 28975488) Youichi Sato, Atsushi Tajima, Motoki Katsurayama, Shiari Nozawa, Miki Yoshiike, Eitetsue Koh, Jiro Kanaya, Mikio Namiki, Kiyomi Matsumiya, Akira Tsujimura, Kiyoshi Komatsu, Naoki Itoh, Jiro Eguchi, Issei Imoto, Aiko Yamauchi and Teruaki Iwamoto :
An independent validation study of three single nucleotide polymorphisms at the sex hormone-binding globulin locus for testosterone levels identified by genome-wide association studies,
Human Reproduction Open, Vol.2017, No.1, 1-8, 2017.

(徳島大学機関リポジトリ): ● Metadata: 111006
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/hropen/hox002
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1093/hropen/hox002

(徳島大学機関リポジトリ: 111006, DOI: 10.1093/hropen/hox002) Kiyotake Yamamoto, Hiroyuki Mizuguchi, Natsumi Tokashiki, Makoto Kobayashi, Motoyuki Tamaki, Youichi Sato, Hiroyuki Fukui and Aiko Yamauchi :
Protein kinase C-δ signaling regulates glucagon secretion from pancreatic islets,
The Journal of Medical Investigation : JMI, Vol.64, No.1,2, 122-128, 2017.

(要約): Accumulating evidence supports the "glucagonocentric hypothesis", in which antecedent -cell failure and inhibition of glucagon secretion are responsible for diabetes progression. Protein kinase C (PKC) is involved in glucagon secretion from -cells, although which PKC isozyme is involved and the mechanism underlying this PKC-regulated glucagon secretion remains unknown. Here, the involvement of PKC in the onset and progression of diabetes was elucidated. Immunofluorescence studies revealed that PKC was expressed and activated in -cells of STZ-induced diabetic model mice. Phorbol 12-myristate 13-acetate (PMA) stimulation significantly augmented glucagon secretion from isolated islets. Pre-treatment with quercetin and rottlerin, PKC signaling inhibitors, significantly suppressed the PMA-induced elevation of glucagon secretion. While Go6976, a Ca(2+)-dependent PKC selective inhibitor did not suppress glucagon secretion. Quercetin suppressed PMA-induced phosphorylation of Tyr(311) of PKC in isolated islets. However, quercetin itself had no effect on either glucagon secretion or glucagon mRNA expression. Our data suggest that PKC signaling inhibitors suppressed glucagon secretion. Elucidation of detailed signaling pathways causing PKC activation in the onset and progression of diabetes followed by the augmentation of glucagon secretion could lead to the identification of novel therapeutic target molecules and the development of novel therapeutic drugs for diabetes. J. Med. Invest. 64: 122-128, February, 2017.
(徳島大学機関リポジトリ): ● Metadata: 111099
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.64.122
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28373608; ● Summary page in Scopus @ Elsevier: 2-s2.0-85016425921

(徳島大学機関リポジトリ: 111099, DOI: 10.2152/jmi.64.122, PubMed: 28373608, Elsevier: Scopus) 十塚正治, The Super Science High School Consortium, 佐藤陽一, 田中雅嗣 :
スーパーサイエンスハイスクール(SSH)コンソーシアムによる日本人のY染色体とミトコンドリアDNAハプログループ地域分布調査,
Anthropological Science (Japanese Series), Vol.124, No.2, 85-91, 2016年.

(出版サイトへのリンク): ● Publication site (DOI): 10.1537/asj.161018
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1537/asj.161018

(DOI: 10.1537/asj.161018) Youichi Sato, Atsushi Tajima, Motoki Katsurayama, Shiari Nozawa, Miki Yoshiike, Eitetsue Koh, Jiro Kanaya, Mikio Namiki, Kiyomi Matsumiya, Akira Tsujimura, Kiyoshi Komatsu, Naoki Itoh, Jiro Eguchi, Issei Imoto, Aiko Yamauchi and Teruaki Iwamoto :
A replication study of a candidate locus for follicle-stimulating hormone levels and association analysis for semen quality traits in Japanese men,
Journal of Human Genetics, Vol.61, No.11, 911-915, 2016.

(要約): In men, follicle-stimulating hormone (FSH) acts on the seminiferous tubules and enhances spermatogenesis. Recently, a candidate locus (rs2414095) for FSH levels was identified by a genome-wide association study (GWAS) in Chinese men. The rs2414095 single-nucleotide polymorphism (SNP) is found on the third intron of the cytochrome P450, family 19, subfamily A, peptide 1 (CYP19A1) gene encoding an aromatase. In the present study, we performed a replication study in 1687 Japanese men (901 from cohort 1 and 786 from cohort 2) to assess whether this SNP is associated with circulating FSH levels. Furthermore, we investigated whether the rs2414095 SNP is correlated with semen quality traits in 2015 Japanese men (1224 from cohort 1 and 791 from cohort 2). The rs2414095 SNP was significantly associated with circulating FSH levels (STD=0.15, P=9.7 × 10(-5)), sperm concentration (STD=0.073, P=0.032) and total sperm number (TSN) (STD=0.074, P=0.027) in a combined analysis of the two Japanese male cohorts. We successfully replicated, in Japanese men, the results of the previous GWAS for the rs2414095 SNP in Chinese men, and found that the rs2414095 SNP was related with sperm production.
(キーワード): Adult / Alleles / Cohort Studies / Follicle Stimulating Hormone / Genetic Association Studies / Genotype / Gonadal Steroid Hormones / Humans / Japan / Male / Polymorphism, Single Nucleotide / Quantitative Trait Loci / Quantitative Trait, Heritable / Semen Analysis / Sperm Count / Sperm Motility
(徳島大学機関リポジトリ): ● Metadata: 109999
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/jhg.2016.82
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27357427; ● Search Scopus @ Elsevier (PMID): 27357427; ● Search Scopus @ Elsevier (DOI): 10.1038/jhg.2016.82

(徳島大学機関リポジトリ: 109999, DOI: 10.1038/jhg.2016.82, PubMed: 27357427) Masaya Denda, Takuya Morisaki, Taiki Kohiki, Jun Yamamoto, Kohei Sato, Ikuko Sagawa, Tsubasa Inokuma, Youichi Sato, Aiko Yamauchi, Akira Shigenaga and Akira Otaka :
Labelling of endogenous target protein via N-S acyl transfer-mediated activation of N-sulfanylethylanilide,
Organic & Biomolecular Chemistry, Vol.14, 6244-6251, 2016.

(徳島大学機関リポジトリ): ● Metadata: 111885
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/C6OB01014H
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1039/C6OB01014H

(徳島大学機関リポジトリ: 111885, DOI: 10.1039/C6OB01014H) Mohammad Jabasini, ASHRAF ABDEL AZIM EWIS, Youichi Sato, Yutaka Nakahori and Yoshinobu Baba :
Anomalous Separation of Small Y-Chromosomal DNA Fragments on Microchip Electrophoresis,
Scientia Pharmaceutica, Vol.84, No.3, 507-513, 2016.

(要約): We investigated an anomalous DNA separation where two DNA fragments from the human Y-chromosome sY638 (64 bp) and sY592 (65 bp), with only one base pair difference, were separated. This result is abnormal since in a previous study, we found that 5 bp was the minimum difference between two DNA fragments that the microchip electrophoresis system can separate. The formation of a mini-loop in the structure of the DNA fragment of sY638 (64 bp) was strongly expected to be the reason. To investigate this, we synthesized three modified DNA fragments for sY638 (64 bp), and the modifications were in two expected locations for possible mini-loop formation. Later, the separation between sY592 (65 bp) and the three modified fragments of sY638 (64 bp) was not possible. Thus, we conclude that the formation of a mini-loop in the structure of the DNA is the reason behind this anomalous separation.
(徳島大学機関リポジトリ): ● Metadata: 115722
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/scipharm84030507
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28117316; ● Search Scopus @ Elsevier (PMID): 28117316; ● Search Scopus @ Elsevier (DOI): 10.3390/scipharm84030507

(徳島大学機関リポジトリ: 115722, DOI: 10.3390/scipharm84030507, PubMed: 28117316) Youichi Sato, Atsushi Tajima, Kouki Tsunematsu, Shiari Nozawa, Miki Yoshiike, Eitetsue Koh, Jiro Kanaya, Mikio Namiki, Kiyomi Matsumiya, Akira Tsujimura, Kiyoshi Komatsu, Naoki Itoh, Jiro Eguchi, Issei Imoto, Aiko Yamauchi and Teruaki Iwamoto :
Lack of replication of four candidate SNPs implicated in human male fertility traits: a large-scale population-based study,
Human Reproduction, Vol.30, No.6, 1505-1509, 2015.

(要約): Are the four candidate loci (rs7867029, rs12870438, rs7174015 and rs724078) for human male fertility traits, identified in a genome-wide association study (GWAS) of a Hutterite population in the USA, associated with semen quality traits in a Japanese population? The four single nucleotide polymorphisms (SNPs) rs7867029, rs12870438, rs7174015 and rs724078 have no association with semen parameters in a meta-analysis of two Japanese male cohorts. Four (rs7867029, rs12870438, rs7174015 and rs724078) of the SNPs associated with family size or birth rate in the GWAS of a Hutterite population in the USA were associated with semen parameters in ethnically diverse men from Chicago, USA. This is a replication study in a total of 2015 Japanese subjects, including 791 fertile men and 1224 young men from the general population. We performed a replication study in two cohorts to assess whether the SNPs rs7867029, rs12870438, rs7174015 and rs724078 are associated with sperm concentration, semen volume, total sperm numbers, total motile sperm numbers or sperm motility. The rs12870438 SNP was detected by restriction fragment length polymorphism PCR while rs7174015, rs724078 and rs7867029 SNPs were genotyped using TaqMan probes. This study indicated that none of the four SNPs rs7867029, rs12870438, rs7174015 and rs724078 displayed a significant association with semen parameters in the meta-analysis of two Japanese male cohorts. Only four SNPs identified in the Hutterite GWAS were examined for associations with semen quality traits in a Japanese population. In addition, the linkage disequilibrium structures around the testing markers were different between ethnic groups. Locus mapping studies using a set of tagging SNPs across the loci will be necessary in populations with larger sample sizes in order to understand the contribution of specific genes to semen quality. This study was supported in part by the Ministry of Health and Welfare of Japan (1013201) (to T.I.), Grant-in-Aids for Scientific Research (C) (23510242) (to A.Ta.) from the Japan Society for the Promotion of Science, the European Union (BMH4-CT96-0314) (to T.I.), and the Takeda Science Foundation (to A.Ta.). None of the authors has any competing interests to declare.
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/humrep/dev081
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25908655; ● Summary page in Scopus @ Elsevier: 2-s2.0-84939638739

(DOI: 10.1093/humrep/dev081, PubMed: 25908655, Elsevier: Scopus) Youichi Sato, Atsushi Tajima, Kouki Tsunematsu, Shiari Nozawa, Miki Yoshiike, Eitetsue Koh, Jiro Kanaya, Mikio Namiki, Kiyomi Matsumiya, Akira Tsujimura, Kiyoshi Komatsu, Naoki Itoh, Jiro Eguchi, Issei Imoto, Aiko Yamauchi and Teruaki Iwamoto :
An association study of four candidate loci for human male fertility traits with male infertility,
Human Reproduction, Vol.30, No.6, 1510-1514, 2015.

(要約): Are the four candidate loci (rs7867029, rs7174015, rs12870438 and rs724078) for human male fertility traits, identified in a genome-wide association study (GWAS) of a Hutterite population in the USA, associated with male infertility in a Japanese population? rs7867029, rs7174015 and rs12870438 are significantly associated with the risk of male infertility in a Japanese population. Recently, a GWAS of a Hutterite population in the USA revealed that 41 single-nucleotide polymorphisms (SNPs) were significantly correlated with family size or birth rate. Of these, four SNPs (rs7867029, rs7174015, rs12870438 and rs724078) were found to be associated with semen parameters in ethnically diverse men from Chicago. This is a case-control association study in a total of 917 Japanese subjects, including 791 fertile men, 76 patients with azoospermia and 50 patients with oligozoospermia. Azoospermia was diagnosed on the basis of semen analysis (the absence of sperm in ejaculate), serum hormone levels and physical examinations. Oligozoospermia was defined as a sperm concentration of <20 × 10(6)/ml. We excluded patients with any known cause of infertility (i.e. obstructive azoospermia, varicocele, cryptorchidism, hypogonadotropic hypogonadism, karyotype abnormalities or complete deletion of AZF a, b or c). The SNPs rs7867029, rs7174015, rs12870438 and rs724078 were genotyped using DNA from peripheral blood samples and either restriction fragment length polymorphism PCR or TaqMan probes. Genetic associations between the four SNPs and male infertility were assessed using a logistic regression analysis under three different comparative models (additive, recessive or dominant). The genotypes of all four SNPs were in Hardy-Weinberg equilibrium in the fertile controls. The SNPs rs7867029 and rs7174015 are associated with oligozoospermia [rs7867029: odds ratio (OR) = 1.70, 95% confidence interval (CI) = 1.07-2.68, P = 0.024 (log-additive); rs7174015: OR = 6.52, 95% CI = 1.57-27.10, P = 0.0099 (dominant)] and rs12870438 is associated with azoospermia (OR = 10.90, 95% CI = 2.67-44.60, P = 0.00087 (recessive)] and oligozoospermia [OR = 8.54, 95% CI = 1.52-47.90, P = 0.015 (recessive)]. The association between rs7174015 and oligozoospermia under a dominant model and between rs12870438 and azoospermia under additive and recessive models remained after correction for multiple testing. There were no associations between rs724078 and azoospermia or oligozoospermia. Even though the sample size of case subjects was not very large, we found that three SNPs were associated with the risk of male infertility in a Japanese population. The three infertility-associated SNPs may be contributing to a quantitative reduction in spermatogenesis. This study was supported in part by the Ministry of Health and Welfare of Japan (1013201) (to T.I.), Grant-in-Aids for Scientific Research (C) (23510242) (to A.Ta.) from the Japan Society for the Promotion of Science, the European Union (BMH4-CT96-0314) (to T. I.) and the Takeda Science Foundation (to A.Ta.). None of the authors has any competing interests to declare.
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/humrep/dev088
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25908656; ● Summary page in Scopus @ Elsevier: 2-s2.0-84939605579

(DOI: 10.1093/humrep/dev088, PubMed: 25908656, Elsevier: Scopus) Youichi Sato, Toshikatsu Shinka, Shiari Nozawa, Miki Yoshiike, Eitetsue Koh, Jiro Kanaya, Mikio Namiki, Kiyomi Matsumiya, Akira Tsujimura, Kiyoshi Komatsu, Naoki Itoh, Jiro Eguchi, Aiko Yamauchi, Teruaki Iwamoto and Yutaka Nakahori :
Y chromosome haplogroup D2a1 is significantly associated with high levels of luteinizing hormone in Japanese men,
Andrology, Vol.3, No.3, 520-525, 2015.

(要約): The association between the Y chromosome haplogroup D2 and risk of azoospermia and low sperm motility has been previously studied, and it was indicated that haplogroups DE (YAP lineage) are associated with prostate cancer risk in Japanese males. Our assumption had been that Y chromosome haplogroups may be associated with sex hormone levels, because sex hormones have been deemed responsible for spermatogenesis and carcinogenesis. In this study, we assessed the association between Y chromosome haplogroups and sex hormone levels, including those of testosterone, sex hormone-binding globulin (SHBG), follicle-stimulating hormone (FSH), luteinizing hormone (LH), inhibin-B, and calculated free testosterone (cFT), in 901 young men from the general Japanese population (cohort 1) and 786 Japanese men of proven fertility (cohort 2). We found that the haplogroup D2a1 was significantly associated with high LH levels in a combined analysis involving two cohorts (β = 0.068, SE = 0.025, p = 0.0075), following correction for multiple testing. To date, this result is the first evidence that implicates Y chromosome haplogroups in an association with sex hormone levels.
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/andr.12026
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25858496; ● Summary page in Scopus @ Elsevier: 2-s2.0-84929842765

(DOI: 10.1111/andr.12026, PubMed: 25858496, Elsevier: Scopus) Youichi Sato, Toshikatsu Shinka, ASHRAF ABDEL AZIM EWIS, Aiko Yamauchi, Teruaki Iwamoto and Yutaka Nakahori :
Overview of genetic variation in the Y chromosome of modern Japanese males,
Anthropological Science, Vol.122, No.3, 131-136, 2014.

(出版サイトへのリンク): ● Publication site (DOI): 10.1537/ase.140709
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84919705410

(DOI: 10.1537/ase.140709, Elsevier: Scopus) Youichi Sato, Takanobu Kamada and Aiko Yamauchi :
The role of dipeptidyl peptidase 4 (DPP4) in the preservation of renal function: DPP4 involvement in hemoglobin expression,
The Journal of Endocrinology, Vol.223, No.2, 133-142, 2014.

(要約): In a previous study, we demonstrated that dipeptidyl peptidase 4 (DPP4)-deficient rats were susceptible to reduced glomerular filtration rate as a result of streptozotocin (STZ)-induced diabetes. Therefore, we proposed that DPP4 might be responsible for the preservation of renal function. In this study, to verify the role of DPP4 in the preservation of renal function, we performed a microarray analysis of the kidneys of WT and DPP4-deficient rats after STZ treatment, and gene expression analysis using rat kidneys, human embryonic kidney 293 (HEK293) cells, and human renal cancer cells (CakI-1). The microarray analysis indicated that the expression levels of the transporter activity, heme-binding, and pheromone binding-related genes changed significantly. The results of gene expression analysis indicated that there were no significant differences in the expression levels of hemoglobin mRNA between the DPP4-deficient and WT rats; however, the expression levels of hemoglobin mRNA in the kidneys of DPP4-deficient rats tended to decrease when compared with those of both the non-STZ-treated and STZ-treated WT rats. The expression levels of hemoglobin in HEK293 and Caki-1 cells were significantly decreased when DPP4 was knocked down by siRNA, were significantly increased by the addition of soluble human DPP4, and were also significantly increased by the addition of the DPP4 inhibitor, sitagliptin. The expression level of DPP4 was also significantly increased by the addition of sitagliptin in both cell types. Our findings indicate that DPP4 regulates the expression of the hemoglobin genes, and might play a role in the preservation of renal function; however, the underlying mechanism of this preservation remains to be elucidated.
(出版サイトへのリンク): ● Publication site (DOI): 10.1530/JOE-14-0016
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25122001; ● Search Scopus @ Elsevier (PMID): 25122001; ● Search Scopus @ Elsevier (DOI): 10.1530/JOE-14-0016

(DOI: 10.1530/JOE-14-0016, PubMed: 25122001) Jun Yamamoto, Nami Maeda, Chiaki Komiya, Tomohiro Tanaka, Masaya Denda, Koji Ebisuno, Wataru Nomura, Hirokazu Tamamura, Youichi Sato, Aiko Yamauchi, Akira Shigenaga and Akira Otaka :
Development of a fluoride-responsive amide bond cleavage device that is potentially applicable to a traceable linker,
Tetrahedron, Vol.70, No.34, 5122-5127, 2014.

(徳島大学機関リポジトリ): ● Metadata: 111908
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.tet.2014.05.110
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84903391352

(徳島大学機関リポジトリ: 111908, DOI: 10.1016/j.tet.2014.05.110, Elsevier: Scopus) Youichi Sato, Iwamoto Teruaki, Toshikatsu Shinka, Nozawa Shiari, Yoshiike Miki, Koh Eitetsue, Kanaya Jiro, Namiki Mikio, Matsumiya Kiyomi, Tsujimura Akira, Komatsu Kiyoshi, Itoh Naoki, Eguchi Jiro, Aiko Yamauchi and Yutaka Nakahori :
Y chromosome gr/gr subdeletion is associated with lower semen quality in young men from the general Japanese population but not in fertile Japanese men,
Biology of Reproduction, Vol.90, No.6, 116, 2014.

(要約): Several case-control studies have investigated whether Y chromosome haplogroups or deletions are associated with spermatogenic failure. However, the relationships between Y chromosome haplogroups or deletions and semen quality in general population have not been elucidated. In this study, we assessed relationships between Y chromosome haplogroups or deletions and semen parameters in 791 fertile Japanese men and 1221 young men from the general Japanese population. We found that the haplogroup D2 (M55 lineage) was significantly associated with lower semen parameters, especially total motile sperm count (P = 0.00051, beta = -0.097), in men from the general population but not in fertile men. In addition, we found that the gr/gr subdeletion was associated with semen quality and in particular, strongly associated with decreased sperm motility (P = 0.00041, beta = -3.14) and total motile sperm count (P = 0.00031, beta = -0.099) in men from the general population but not in fertile men. The combined analysis of fertile Japanese men and men from the general Japanese population showed that the haplogroup D2 (M55 lineage) and the gr/gr subdeletion were strongly associated with reduced sperm motility (P = 0.00056, beta = -2.71, and P = 7.7 × 10(-5), beta = -3.05, respectively) and that haplogroup O2b1 was strongly associated with elevated sperm motility (P = 0.00089, beta = 2.94). These observations add further support for the view that the gr/gr subdeletion diminishes sperm motility that consequently may result in male infertility.
(キーワード): Adolescent / Adult / Asian Continental Ancestry Group / Chromosome Deletion / Chromosomes, Human, Y / Female / Fertility / Haplotypes / Humans / Infertility, Male / Japan / Male / Middle Aged / Pregnancy / Prevalence / Semen Analysis / Spermatozoa / Young Adult
(出版サイトへのリンク): ● Publication site (DOI): 10.1095/biolreprod.114.118810
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24759788; ● Summary page in Scopus @ Elsevier: 2-s2.0-84903756731

(DOI: 10.1095/biolreprod.114.118810, PubMed: 24759788, Elsevier: Scopus) Mai Nagao, Youichi Sato and Aiko Yamauchi :
Meta-Analysis of Interleukin Polymorphisms and NSAID Usage Indicates Correlations to the Risk of Developing Cancer,
International Journal of Genomic Medicine, Vol.2, No.1, 1000113, 2014.

(出版サイトへのリンク): ● Publication site (DOI): 10.4172/2332-0672.1000113
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.4172/2332-0672.1000113

(DOI: 10.4172/2332-0672.1000113) Nagao Mai, Youichi Sato and Aiko Yamauchi :
A meta-analysis of the association of PPARγ rs1801282 polymorphism and NSAID usage with the risk of developing cancer,
Biological & Pharmaceutical Bulletin, Vol.37, No.6, 1062-1067, 2014.

(要約): Use of nonsteroidal anti-inflammatory drugs (NSAIDs) is correlated with a reduced risk of cancer through the reduction of inflammation, which is an important risk factor. Several studies have investigated polymorphisms in the peroxisome proliferator-activated receptor gamma (PPARγ) gene and NSAID use in association with cancer risk. However, these studies yielded mixed results. Therefore, we performed a meta-analysis to evaluate the association of PPARγ polymorphisms and NSAID usage with cancer risk. We conducted a comprehensive search of PubMed through May 2013. Odds ratios (ORs) with corresponding 95% confidence intervals (CIs) were calculated using the fixed-effect or random-effect model. A comprehensive search of the database revealed 6 studies that fulfilled the inclusion criteria. NSAID use was significantly associated with decreased cancer risk regardless of PPARγ rs1801282 genotypes. In a stratified analysis by cancer type, NSAID users who were minor allele carriers had significantly decreased colon cancer risk compared to non-NSAID users (OR=0.73, 95% CI=0.57-0.93), whereas NSAID users homozygous for the major allele had significantly decreased risk for cancers other than colon cancer compared to non-NSAID users (OR=0.79, 95% CI=0.69-0.91). Our results suggest that the association of PPARγ rs1801282 polymorphism and NSAID use with the risk of cancer may differ according to cancer type.
(キーワード): Alleles / Anti-Inflammatory Agents, Non-Steroidal / Female / Genotype / Homozygote / Humans / Incidence / Male / Neoplasms / PPAR gamma / Polymorphism, Genetic / Risk
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.b14-00160
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24882418; ● CiNii @ 国立情報学研究所 (CRID): 1390282679607989632; ● Summary page in Scopus @ Elsevier: 2-s2.0-84901839094

(DOI: 10.1248/bpb.b14-00160, PubMed: 24882418, CiNii: 1390282679607989632, Elsevier: Scopus) Jun Yamamoto, Masaya Denda, Nami Maeda, Miku Kita, Chiaki Komiya, Tomohiro Tanaka, Wataru Nomura, Hirokazu Tamamura, Youichi Sato, Aiko Yamauchi, Akira Shigenaga and Akira Otaka :
Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins,
Organic & Biomolecular Chemistry, Vol.12, No.23, 3821-3826, 2014.

(要約): A traceable linker that is potentially applicable to identification of a target protein of bioactive compounds was developed. It enabled not only thiol-induced cleavage of the linker for enrichment of the target protein but also selective labelling to pick out the target from contaminated non-target proteins for facile identification.
(キーワード): Amides / Amino Acids / Click Chemistry / Electrophoresis, Polyacrylamide Gel / Peptides / Proteins / Staining and Labeling / Sulfhydryl Compounds
(徳島大学機関リポジトリ): ● Metadata: 111911
(出版サイトへのリンク): ● Publication site (DOI): 10.1039/C4OB00622D
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24806338; ● Summary page in Scopus @ Elsevier: 2-s2.0-84901285801

(徳島大学機関リポジトリ: 111911, DOI: 10.1039/C4OB00622D, PubMed: 24806338, Elsevier: Scopus) Mai Nagao, Youichi Sato and Aiko Yamauchi :
A Meta-Analysis of PTGS1 and PTGS2 Polymorphisms and NSAID Intake on the Risk of Developing Cancer,
PLoS ONE, Vol.8, No.8, e71126, 2013.

(要約): Several studies have investigated whether the polymorphisms in the prostaglandin endoperoxide synthase 1 (PTGS1) and PTGS2 genes and nonsteroidal anti-inflammatory drug (NSAID) use are associated with cancer risk; however, those studies have produced mixed results. Therefore, we performed a meta-analysis to evaluate the association between the PTGS1 and PTGS2 polymorphisms and the effect of NSAID use on the risk of developing cancer. We conducted a comprehensive search in PubMed through March 2012. The odds ratios (ORs) with the corresponding 95% confidence intervals (CIs) were calculated using the fixed-effect model or the random-effect model. The database search generated 13 studies that met the inclusion criteria. For PTGS1 rs3842787, NSAID users homozygous for the major allele (CC) had a significantly decreased cancer risk compared with non-NSAID users (OR = 0.73, 95% CI = 0.59-0.89). For PTGS2 rs5275 and rs20417, there were no significant differences between the gene polymorphism and NSAID use on cancer risk among the 8 and 7 studies, respectively. However, in the stratified analysis by the type of cancer or ethnicity population, NSAID users homozygous for the major allele (TT) in rs5275 demonstrated significantly decreased cancer risk compared with non-NSAID users in cancer type not involving colorectal adenoma (OR = 0.70, 95% CI = 0.59-0.83) and among the USA population (OR = 0.67, 95% CI = 0.56-0.82). NSAID users homozygous for the major allele (GG) in rs20417 displayed a significantly decreased cancer risk than non-NSAID users among the US population (OR = 0.72, 95% CI = 0.58-0.88). For the PTGS2 rs689466 and rs2745557 SNPs, there were no significant differences. This meta-analysis suggests that the associations between PTGS polymorphisms and NSAID use on cancer risk may differ with regard to the type of cancer and nationality.
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0071126
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23967159; ● Search Scopus @ Elsevier (PMID): 23967159; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0071126

(DOI: 10.1371/journal.pone.0071126, PubMed: 23967159) Youichi Sato, Toshikatsu Shinka, Teruaki Iwamoto, Aiko Yamauchi and Yutaka Nakahori :
Y chromosome haplogroup D2* lineage is associated with azoospermia in Japanese males,
Biology of Reproduction, Vol.88, No.4, 107, 2013.

(要約): Several studies have investigated whether particular Y chromosome haplogroups are associated with spermatogenic failure in Japanese males; however, they produced differing results. In this study, to investigate the association of Y chromosome haplogroup with spermatogenic failure, we recruited 451 infertile patients and 730 fertile men from a Japanese population and typed their Y chromosome haplogroups. The infertile patients were suffering from varicocele, azoospermia, oligozoospermia, asthenozoospermia, obstructive azoospermia, karyotype abnormalities, microdeletions of the long arm of the Y chromosome, or other conditions that affect fertility. The frequency of haplogroup D2* was significantly higher (odds ratio = 2.28, 95% confidence interval = 1.44-3.61, P = 0.00034 using chi-square test) among the men with azoospermia than among the fertile men. None of the other Y haplogroups displayed associations with particular types of infertility. In conclusion, Y chromosome haplogroup D2* is associated with spermatogenic failure in Japanese males, suggesting that the Y chromosome lineage can have significant effects on spermatogenesis.
(キーワード): Adult / Asian Continental Ancestry Group / Azoospermia / Case-Control Studies / Cell Lineage / Chromosome Deletion / Chromosomes, Human, Y / DNA Mutational Analysis / Haplotypes / Humans / Japan / Male / Phylogeny / Spermatogenesis
(出版サイトへのリンク): ● Publication site (DOI): 10.1095/biolreprod.112.105718
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23467741; ● Search Scopus @ Elsevier (PMID): 23467741; ● Search Scopus @ Elsevier (DOI): 10.1095/biolreprod.112.105718

(DOI: 10.1095/biolreprod.112.105718, PubMed: 23467741) Youichi Sato, Timothy Jinam, Teruaki Iwamoto, Aiko Yamauchi, Issei Imoto, Ituro Inoue and Atsushi Tajima :
Replication study and meta-analysis of human nonobstructive azoospermia in Japanese populations,
Biology of Reproduction, Vol.88, No.4, 87, 2013.

(要約): Recently, a Chinese genomewide association study (GWAS) identified four autosomal single-nucleotide polymorphism (SNP) loci as being significantly associated with risk factors for nonobstructive azoospermia (NOA; P < 5 × 10(-8)). In the present study, we performed a replication study on two Japanese cohorts from different institutions in order to evaluate whether SNP loci are associated with NOA. The four SNPs (rs12097821, rs2477686, rs10842262, and rs6080550) reported in the Chinese GWAS were genotyped in 490 NOA patients and 1167 controls. To assess the significance of the associations between each of the four SNPs and NOA in the Japanese population, the association results for the two cohorts were combined by meta-analysis. In the meta-analysis, the combined per-allele odds ratios (ORs) for the four SNPs and their respective 95% confidence intervals (CIs) were as follows: rs12097821, OR = 1.10 (CI = 0.89-1.37); rs2477686, OR = 1.11 (CI = 0.87-1.43); rs10842262, OR = 1.11 (CI = 0.94-1.32); and rs6080550, OR = 0.96 (CI = 0.76-1.21). None of the SNPs was significantly associated with NOA (P > 0.05). However, three of four SNPs (rs12097821, rs2477686, and rs10842262) showed associations in the same direction in Japanese men as those reported in the Chinese GWAS. To determine whether the four SNPs are genetic risk factors for NOA, the effect sizes of NOA risk factors require further investigation using larger independent sets of case-control samples of populations, including Japanese and Chinese populations.
(キーワード): Adult / Asian Continental Ancestry Group / Azoospermia / Case-Control Studies / Cohort Studies / Gene Frequency / Genome-Wide Association Study / Genotype / Humans / Japan / Male / Polymorphism, Single Nucleotide
(出版サイトへのリンク): ● Publication site (DOI): 10.1095/biolreprod.112.106377
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23467743; ● Search Scopus @ Elsevier (PMID): 23467743; ● Search Scopus @ Elsevier (DOI): 10.1095/biolreprod.112.106377

(DOI: 10.1095/biolreprod.112.106377, PubMed: 23467743) Yasushi Kirino, Masako Sei, Kazuyoshi Kawazoe, Kazuo Minakuchi and Youichi Sato :
Plasma dipeptidyl peptidase 4 activity correlates with body mass index and the plasma adiponectin concentration in healthy young people,
Endocrine Journal, Vol.59, No.10, 949-953, 2012.

(要約): We previously found that plasma dipeptidyl peptidase 4 (DPP4) activity was associated with the development of obesity, type 2 diabetes, and type 1 diabetes using animal models. In this study, we investigated whether DPP4 activity is correlated with the clinical parameters of obesity and/or diabetes in healthy young subjects. Body mass index (BMI), plasma DPP4 activity, total cholesterol, high density lipoprotein (HDL) cholesterol, triglycerides, fasting blood glucose, adiponectin concentration, and body fat were measured in 165 subjects (110 males and 55 females, age 23.2 ± 2.4 years). In correlation analyses, DPP4 activity displayed strong positive correlations with BMI (p = 5.5 × 10(-5)) and total cholesterol (p = 0.0014), and a negative correlation with the plasma adiponectin concentration (p = 0.013), but not fasting blood glucose. Our findings suggest that plasma DPP4 activity is correlated with the clinical parameters of obesity rather than diabetes in young people.
(出版サイトへのリンク): ● Publication site (DOI): 10.1507/endocrj.EJ12-0158
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22785237; ● Search Scopus @ Elsevier (PMID): 22785237; ● Search Scopus @ Elsevier (DOI): 10.1507/endocrj.EJ12-0158

(DOI: 10.1507/endocrj.EJ12-0158, PubMed: 22785237) Yutaka Nakahori, Youichi Sato, ASHRAF ABDEL AZIM EWIS, Teruaki Iwamoto, Toshikatsu Shinka, Shiari Nozawa, Miki Yoshiike, Xin-Jun Yang, Masako Sei, Mikio Namiki, Eitetsu Kou, Naoki Ito, Kiyoshi Komatsu, Kiyomi Matsumiya and Yasuo Nakagome :
Climatic influence on the reproductive characteristics of Japanese males,
Journal of Human Genetics, Vol.57, No.6, 375-378, 2012.

(要約): We previously performed a survey of the sperm characteristics of the partners of pregnant women in four cities in Japan. In the present study, we analyzed the sperm characteristics of these subjects and the correlations between these sperm characteristics and climatic changes or Y chromosome haplogroups. Our results showed that more haplogroup D2a1 males than O2b1 males were born in the first half of the year (January to June), whereas more O2b1 males were born in the last half of the year (July to December) (P<0.05). This was agreed and correlated with the seasonal variations in their mean sperm concentrations. The haplogroup C, D* and D2a1 males displayed lower sperm concentrations from March to May, followed by an increase in their sperm concentrations starting in June or July, while the O2b1 males displayed higher sperm concentrations in the first half of the year followed by a sudden decrease from July to August (P<0.05). We hypothesize that the Japanese climate has different effects on the sperm characteristics and reproductive seasonality of males from different lineages; and therefore, has influenced the modern population of Japan.
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/jhg.2012.38
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22534769; ● Search Scopus @ Elsevier (PMID): 22534769; ● Search Scopus @ Elsevier (DOI): 10.1038/jhg.2012.38

(DOI: 10.1038/jhg.2012.38, PubMed: 22534769) Kazuyoshi Kawazoe, Kana Ota, Mayuko Okamoto, Youichi Sato and Kazuo Minakuchi :
Anti-obesity effects of Schisandra chinensis fruit water extract in rats fed a high-fat diet,
Journal of Traditional Medicines, Vol.29, No.3, 143-148, 2012.

(要約): 肥満は社会的問題となっており，予防対策に関する研究が進められている．本研究では，五味子 (チョウセンゴミシ果実) の肥満予防に対する有効性について検討した．五味子水抽出エキスを高脂肪食に混合してラットに投与し，肥満に関わるパラメーターに対する影響を検討した． 10週間自由摂食させた結果，摂食量は変わらないにもかかわらず五味子水抽出エキス混合食投与群では高脂肪食のみ投与されていた群より有意な体重減少，内臓脂肪量の減少，血漿中のトリグリセリド減少が見られた．さらに，五味子水抽出液混合食を投与した群では，高脂肪食のみ投与した群と比較して脂肪組織は普通食摂食群と同程度に小さく，ブドウ糖負荷試験において90分後の血糖値は高脂肪食投与群より有意に低かった．以上のことより五味子水抽出エキスは肥満の予防に有効である可能性が示唆された．
(キーワード): traditional medicine / anti-obesity effect / schisandra fruit / epididymal fat / high-calorie diet
(出版サイトへのリンク): ● Publication site (DOI): 10.11339/jtm.29.143
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205234375424; ● Summary page in Scopus @ Elsevier: 2-s2.0-85024448611

(DOI: 10.11339/jtm.29.143, CiNii: 1390001205234375424, Elsevier: Scopus) Youichi Sato, Yano Shojiro, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori :
SRY interacts with ribosomal proteins S7 and L13a in nuclear speckles.,
Cell Biology International, Vol.35, No.5, 449-452, 2011.

(要約): The SRY (sex-determining region on the Y chromosome) is essential for male development; however, the molecular mechanism by which the SRY induces testis development is still unclear. To elucidate the mechanism of testis development, we identified SRY-interacting proteins using a yeast two-hybrid system. We found two ribosomal proteins, RPS7 (ribosomal protein S7) and RPL13a (ribosomal protein L13a) that interact with the HMG (high-mobility group) box domain of SRY. Furthermore, we confirmed the intracellular distributions of RPS7, RPL13a and SRY and found that the three proteins were co-expressed in COS1 cells. SRY, RPS7 and RPL13a were co-localized in nuclear speckles. These findings suggest that SRY plays an important role in activities associated with nuclear speckles via an unknown mechanism.
(キーワード): Animals / COS Cells / Cercopithecus aethiops / Humans / Male / Ribosomal Proteins / Sex-Determining Region Y Protein / Testis / Two-Hybrid System Techniques / Y Chromosome
(出版サイトへのリンク): ● Publication site (DOI): 10.1042/CBI20090201
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21114473; ● Search Scopus @ Elsevier (PMID): 21114473; ● Search Scopus @ Elsevier (DOI): 10.1042/CBI20090201

(DOI: 10.1042/CBI20090201, PubMed: 21114473) Yasushi Kirino, Youichi Sato, Takayuki Kamimoto, Kazuyoshi Kawazoe and Kazuo Minakuchi :
Altered dipeptidyl peptidase 4 activity during the progression of hyperinsulinemic obesity and islet atrophy in spontaneously late-stage type 2 diabetic rats.,
American Journal of Physiology, Endocrinology and Metabolism, Vol.300, No.2, 372-379, 2011.

(要約): Altered dipeptidyl peptidase-4 (DPP4) activity during the progression of late-stage type 2 diabetes was measured in Otsuka Long-Evans Tokushima fatty (OLETF) rats. Compared with OLETF rats subjected to 30% food restriction, food-satiated OLETF rats exhibited spontaneous hyperphagic obesity, insulin resistance, hyperglycemia, hyperinsulinemia, and increased plasma DPP4 activity during the early phase of the experiment (up to ∼30 wk). Subsequently, their plasma DPP4 activity as well as their body weight, body fat, and plasma insulin concentration declined to control levels during the late phase, resulting in excessive polyuria, proteinuria, dyslipidemia, pancreatic islet atrophy, hypoinsulinemia, and diabetes, which changed from insulin-resistant diabetes to hypoinsulinemic diabetes secondary to progressive islet insufficiency, and their fasting blood glucose level remained high. Since plasma DPP4 activity demonstrated significant positive correlations with body weight and the fasting plasma insulin level but not with the fasting blood glucose level during the late stage of diabetes, body fat and fasting plasma insulin levels may be useful factors for predicting the control of plasma DPP4 activity. In contrast, pancreatic DPP4 activity was significantly increased, and the expression of pancreatic insulin was significantly reduced in late-stage diabetic OLETF rats, suggesting that a relationship exists between the activation of pancreatic DPP4 and insulin depletion in pancreatic islet atrophy. In conclusion, it is suggested that plasma DPP4 activity changes in accordance with the progression of hyperinsulinemic obesity and pancreatic islet atrophy. DPP4 activity may play an important role in insulin homeostasis.
(キーワード): Animals / Area Under Curve / Atrophy / Blood Glucose / Body Weight / Diabetes Mellitus, Type 2 / Dipeptidyl Peptidase 4 / Disease Progression / Food Deprivation / Glucose Tolerance Test / Hyperinsulinism / Immunohistochemistry / Insulin / Islets of Langerhans / Male / Obesity / Organ Size / Proteinuria / Rats / Rats, Inbred OLETF / Reverse Transcriptase Polymerase Chain Reaction / Satiation
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpendo.00319.2010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21139073; ● Search Scopus @ Elsevier (PMID): 21139073; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpendo.00319.2010

(DOI: 10.1152/ajpendo.00319.2010, PubMed: 21139073) Youichi Sato, Sakura Kosioka, Yasushi Kirino, Takayuki Kamimoto, Kazuyoshi Kawazoe, Shinji Abe, Kazuo Minakuchi and Yutaka Nakahori :
Role of dipeptidyl peptidase IV (DPP4) in the development of dyslipidemia: DPP4 contributes to the steroid metabolism pathway.,
Life Sciences, Vol.88, No.1-2, 43-49, 2011.

(要約): We previously reported that dipeptidyl peptidase IV (DPP4)-deficient rats were susceptible to dyslipidemia induced by streptozotocin (STZ). Hence, it is suggested that DPP4 is important for lipid metabolism. In this study, to verify the role of DPP4 in the development of dyslipidemia, we carried out a microarray analysis of the livers of STZ-treated wild-type and DPP4-deficient rats and showed that the expression levels of genes involved in metabolic processes (steroid metabolic processes and cellular lipid metabolic processes) were significantly altered by STZ treatment. In the wild-type rats, the expression of hydroxysteroid (17-beta) dehydrogenase 2 (Hsd7b2), which catalyzes sex steroid synthesis from cholesterol, was significantly increased by about 15-fold after STZ treatment; however, it did not change in the DPP4-deficient rats. In the STZ untreated group of DPP4-deficient rats, the expression levels of cytochrome P450, subfamily 51 (Cyp51) and sterol-C4-methyl oxidase-like (Sc4mol), which catalyze intermediate steps in cholesterol synthesis, were significantly elevated compared to those of other groups. Similar results were demonstrated in HuH7-cells after DPP4 overexpression or the addition of human sera containing DPP4. DPP4 is crucial for regulating the expression of factors related to steroid metabolism such as Cyp51, Sc4mol, and Hsd17b2, and DPP4 deficiency or inhibition may cause dyslipidemia.
(キーワード): Animals / Cell Line / Cholesterol / Dipeptidyl Peptidase 4 / Dyslipidemias / Estradiol Dehydrogenases / Humans / Lipid Metabolism / Liver / Male / Oligonucleotide Array Sequence Analysis / Rats / Rats, Inbred F344 / Reverse Transcriptase Polymerase Chain Reaction / Sterol 14-Demethylase / Streptozocin
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.lfs.2010.10.019
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21047519; ● Search Scopus @ Elsevier (PMID): 21047519; ● Search Scopus @ Elsevier (DOI): 10.1016/j.lfs.2010.10.019

(DOI: 10.1016/j.lfs.2010.10.019, PubMed: 21047519) Youichi Sato, Toshikatsu Shinka, Kozue Sakamoto, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori :
The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2.,
Molecular and Cellular Biochemistry, Vol.337, No.1-2, 267-275, 2010.

(要約): In mammals, sex is determined by the presence or absence of the Y chromosome that bears a male-dominant sex-determining gene SRY, which switches the differentiation of gonads into male testes. The molecular signaling mechanism turning on the switch, however, has remained unclear for 18 years since the identification of the gene. Here, we describe how this gene emerged and started to work. From amino acid homology, we realized that SRY is a hybrid gene between a portion of the first exon of DiGeorge syndrome critical region gene 8 (DGCR8) and the high-mobility group (HMG) box of SRY box-3 (SOX3) gene. We identified the regulatory sequence in the SRY promotor region by searching for a common motif shared with DGCR8 mRNA. From the motif search between DGCR8 mRNA and the SRY upstream sequence, we found that the transcription factor CP2 (TFCP2) binding motif is present in both. TFCP2 overexpression did not show a significant increase of SRY mRNA expression, and TFCP2 suppression by RNA interference (RNAi) significantly reduced SRY mRNA expression. Furthermore, electrophoretic mobility shift assay (EMSA) demonstrated that TFCP2 acts as a regulator by directly binding to the SRY promoter. We conclude that SRY is a hybrid gene composed of two genes, DGCR8 and SOX3; and TFCP2 is an essential transcription factor for SRY expression regulation.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11010-009-0308-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19902333; ● Search Scopus @ Elsevier (PMID): 19902333; ● Search Scopus @ Elsevier (DOI): 10.1007/s11010-009-0308-x

(DOI: 10.1007/s11010-009-0308-x, PubMed: 19902333) Hokuma Munakata, Masako Sei, ASHRAF ABDEL AZIM EWIS, Mayumi Umeno, Youichi Sato, Takuro Nakano, Kozue Sakamoto, Yukiko Yoshida, Chiemi Onishi and Yutaka Nakahori :
Prediction of Japanese children at risk for complications of childhood obesity: gender differences for intervention approaches.,
The Journal of Medical Investigation : JMI, Vol.57, No.1-2, 62-68, 2010.

(要約): Childhood obesity is one of the most serious public health problems in Japan, especially in Tokushima compared with other prefectures. This study was designed to clarify the life habits which predispose to development of obesity and can be modified through an appropriate intervention program to combat childhood obesity and its lifestyle-related diseases. A total of 216 school children from Itano Town, a municipality of Tokushima Prefecture, Japan, who are attending the fourth grade (9-10 years) of elementary schools, participated in the study from 2004 to 2007. The study included child's life habits questionnaire, investigating physical activity by recording the daily steps using a pedometer, anthropometric measurements, hematological examination and hemodynamometry in a cross-sectional survey during a two-month period from June to July every year. We conclude that there are considerable gender-related differences for developing obesity and other lifestyle-related diseases; and all intervention strategies against obesity must consider such gender differences. For example, restriction of television watching hours must be intervened for controlling obesity in boys, however for girls, promotion of exercise practice or making more steps per day with adequate sleeping periods should be intervened as the proper approaches for preventing and controlling obesity and other lifestyle-related diseases.
(キーワード): Body Mass Index / 子ども (children) / 肥満症 (obesity) / リスク (risk) / Sex Characteristics
(徳島大学機関リポジトリ): ● Metadata: 68448
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.57.62
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20299744; ● Search Scopus @ Elsevier (PMID): 20299744; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.57.62

(徳島大学機関リポジトリ: 68448, DOI: 10.2152/jmi.57.62, PubMed: 20299744) Kozue Sakamoto, Youichi Sato, Masako Sei, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori :
Proteasome activity correlates with male BMI and contributes to the differentiation of adipocyte in hADSC,
Endocrine, Vol.37, No.2, 274-279, 2010.

(要約): We have previously reported that 26S proteasome subunit mRNA expressions correlate with male body mass index (BMI). In this study, to investigate whether proteasome activities are correlated with BMI, we recruited 61 healthy young Japanese male subjects, measured proteasome activities in their plasma, and correlated them with their BMI and various metabolic factors. We found that among three different proteasome activities, chymotrypsin-like activity in plasma was positively correlated with BMI in healthy Japanese male subjects. Furthermore, we analyzed proteasome activity in vitro during the differentiation of human adipose-derived stem cell (hADSC) into mature adipocytes. In the early stage of differentiation, proteasome activity was at its highest level, and proteasome inhibitor could inhibit hADSC adipocyte differentiation. Our findings suggest that proteasome is an important controlling factor for the development of obesity and adipogenesis.
(キーワード): Adipocytes / Adipogenesis / Adult / Asian Continental Ancestry Group / Body Mass Index / Cell Differentiation / Cell Survival / Cells, Cultured / Cysteine Proteinase Inhibitors / Humans / Leupeptins / Male / Obesity / Proteasome Endopeptidase Complex / Stem Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12020-009-9298-4
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20960262; ● Search Scopus @ Elsevier (PMID): 20960262; ● Search Scopus @ Elsevier (DOI): 10.1007/s12020-009-9298-4

(DOI: 10.1007/s12020-009-9298-4, PubMed: 20960262) 中山知彦, 藤井翔太郎, 勢井雅子, 中野卓郎, エヴィス ABDEL AZIM アシュラフ, 佐藤陽一, 坂本梢, 吉田友紀子 :
日本の子どもの肥満は増加しているのか(平成1∼20年度),
四国公衆衛生学会雑誌, Vol.55, No.1, 143-149, 2010年.

(キーワード): 学校保健

Youichi Sato, Toshikatsu Shinka, G Chen, HT Yan, K Sakamoto, ASHRAF ABDEL AZIM EWIS, H Aburatani and Yutaka Nakahori :
Proteomics and transcriptome approaches to investigate the mechanism of human sex determination,
Cell Biology International, Vol.33, No.8, 839-847, 2009.

(要約): The SRY gene (sex-determining region on the Y chromosome) was isolated in 1990 and is known as the testis-determining factor on the Y chromosome. The SRY has been considered as a transcription factor since it contains an HMG box, which functions as a DNA-binding domain. However, a direct target for SRY remains to be identified. We have investigated the function of SRY through proteomics and transcriptome approaches, and by using two stable SRY-overexpressing cell lines (SRY1 and SRY2) in NT2/D1 cells derived from human testicular embryonal cell carcinoma. The results of 2-dimensional gel electrophoresis show that SRY overexpression causes a considerable downregulation of many chaperone proteins. SRY also upregulates laminin, which is important for Sertoli cell differentiation. Additionally, transcriptome analysis shows that SRY overexpression upregulates many zinc finger proteins and downregulates cellular growth factors with S or G(2)/M arrest of the cell cycle and inhibition of cellular proliferation.
(キーワード): Cell Division / Cell Line, Tumor / Chromosomes, Human, Y / G2 Phase / Gene Expression Profiling / Genes, sry / Humans / Laminin / Proteomics / S Phase / Sex Determination Processes
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.cellbi.2009.04.019
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19427385; ● Search Scopus @ Elsevier (PMID): 19427385; ● Search Scopus @ Elsevier (DOI): 10.1016/j.cellbi.2009.04.019

(DOI: 10.1016/j.cellbi.2009.04.019, PubMed: 19427385) Kozue Sakamoto, Youichi Sato, Toshikatsu Shinka, Masako Sei, Isoko Nomura, Mayumi Umeno, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori :
Proteasome subunits mRNA expressions correlate with male BMI; implications for a role in obesity,
Obesity, Vol.17, No.5, 1044-1049, 2009.

(要約): Obesity as well as its associated chronic diseases and adverse health consequences such as type 2 diabetes mellitus, dyslipidemia, hypertension, and coronary artery disease are afflicting middle-aged adults and an ever greater number of children globally. We planned to investigate new obesity-related factors using proteomics approaches in a randomly selected three high and three low BMI samples of Epstein-Barr-transformed B (EBV-B) lymphoblastoid cell lines prepared from two groups of young Japanese men with different BMI. To search novel obesity-related factors, comparisons of protein expressions between high and low BMI groups were carried out by two-dimensional gel electrophoresis (2-DE). Gene transcripts of proteasome subunits found out from 2-DE were further determined by quantitative real-time PCR. Results from proteomics approach showed that the expression of proteasome alpha subunit type 5 (PSMA5) was significantly lower in the high BMI male group than in those with low BMI (P < 0.05). To validate these results, we expanded the study to include 20 more men and used real-time PCR to quantify the mRNA expression level in their EBV-B cells. Both PSMA5 and PSMA2 of EBV-B cells showed negative correlation with BMI. Furthermore, the mRNA levels measured in the peripheral blood B lymphocytes for many proteasome subunits in 75 healthy men and women showed significant negative correlation with BMI in healthy men. Our findings suggest that proteasome expression may play a key role in obesity.
(キーワード): Body Mass Index / Cell Line / DNA Primers / Female / Herpesvirus 4, Human / Humans / Male / Obesity / Polymerase Chain Reaction / Proteasome Endopeptidase Complex / Protein Subunits / RNA, Messenger / Reference Values / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/oby.2008.612
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19165168; ● Search Scopus @ Elsevier (PMID): 19165168; ● Search Scopus @ Elsevier (DOI): 10.1038/oby.2008.612

(DOI: 10.1038/oby.2008.612, PubMed: 19165168) Yasushi Kirino, Takayuki Kamimoto, Youichi Sato, Kazuyoshi Kawazoe, Kazuo Minakuchi and Yutaka Nakahori :
Increased Plasma Dipeptidyl Peptidase IV (DPP IV) Activity and Decreased DPP IV Activity of Visceral But Not Subcutaneous Adipose Tissue in Impaired Glucose Tolerance Rats Induced by High-Fat or High-Sucrose Diet,
Biological & Pharmaceutical Bulletin, Vol.32, No.3, 463-467, 2009.

(要約): Several studies have investigated whether dipeptidyl peptidase IV (DPP IV) activity is correlated to the severity of diabetes; however, it remains unclear. To investigate the roles of DPP IV activity in metabolic abnormalities, impaired glucose tolerance rats were produced using a high-fat (HF) or high-sucrose (HS) diet. HF diet-fed rats obviously exhibited impaired glucose tolerance, with increases in subcutaneous and epididymal fat mass, insulin resistance and dyslipidaemia. In rats fed a HS diet rather than a normal diet, lower body weight and fasting blood glucose were observed temporarily in the early period after HS diet feeding; however, impaired glucose tolerance was evoked to some extent with an increase in epididymal fat mass. Both HF and HS diet-fed rats showed significantly higher plasma DPP IV activity than normal diet-fed rats, in the order of HF diet>HS diet>normal diet. HF and HS diets did not significantly affect DPP IV activity and mRNA expression in the kidney. On the other hand, HF, but not HS, diet caused a significant decrease in DPP IV activity in the liver as compared to the control. Of note, both HF and HS diets caused a significant decrease in DPP IV activity in epididymal fat, even though they did not change DPP IV activity in subcutaneous fat. In conclusion, HF or HS diet-induced impaired glucose tolerance with visceral fat accumulation may be interrelated with increased plasma DPP IV activity and decreased DPP IV activity of visceral but not subcutaneous adipose tissue.
(キーワード): Animals / Body Weight / Dietary Fats / Dietary Sucrose / Dipeptidyl Peptidase 4 / Eating / Glucose Intolerance / Glucose Tolerance Test / Intra-Abdominal Fat / Male / Organ Specificity / RNA / Rats / Rats, Inbred F344 / Subcutaneous Fat
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.32.463
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19252296; ● Search Scopus @ Elsevier (PMID): 19252296; ● Search Scopus @ Elsevier (DOI): 10.1248/bpb.32.463

(DOI: 10.1248/bpb.32.463, PubMed: 19252296) Youichi Sato, Kozue Sakamoto, Masako Sei, ASHRAF ABDEL AZIM EWIS and Yutaka Nakahori :
Proteasome subunits are regulated and expressed in comparable concentrations as a functional cluster,
Biochemical and Biophysical Research Communications, Vol.378, No.4, 795-798, 2009.

(要約): The proteasome is the main proteolytic enzyme that functions in the ubiquitin-proteasome system. The 26S proteasome has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits; however, it is unclear how these subunits are regulated and the 26S proteasomes assembled. To verify whether each subunit's mRNA expression is associated with the mRNA expression of other proteasome subunits, we carried out expression analysis of 34 proteasome subunits mRNA on peripheral blood from 75 subjects. The expression of proteasome subunits mRNA was comparable in each individual of the studied population and the mRNA expression has been investigated in each 20S or 19S proteasome. Our results suggest that each type of subunit is regulated by respectively common factors, and that the 20S and 19S proteasomes are regulated by different systems.
(キーワード): Female / Humans / Male / Proteasome Endopeptidase Complex / Protein Subunits / RNA, Messenger
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2008.11.125
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19063865; ● Search Scopus @ Elsevier (PMID): 19063865; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2008.11.125

(DOI: 10.1016/j.bbrc.2008.11.125, PubMed: 19063865) Yasushi Kirino, Youichi Sato, Takayuki Kamimoto, Kazuyoshi Kawazoe, Kazuo Minakuchi and Yutaka Nakahori :
Interrelationship of dipeptidyl peptidase IV (DPP IV) with the development of diabetes, dyslipidaemia and nephropathy: A streptozotocin-induced model using wild-type and DPP IV-deficient rats,
The Journal of Endocrinology, Vol.200, No.1, 53-61, 2009.

(要約): We examined the role of dipeptidyl peptidase IV (DPP4) in the development of diabetes, dyslipidaemia and renal dysfunction induced by streptozotocin (STZ). F344/DuCrlCrlj rats, which lack DPP4 activity, and wild-type rats were treated with STZ. Plasma DPP4 activity and biochemical parameters were measured until 42 days after STZ treatment. At the end of the experiment, renal function and DPP4 expressions of the kidney, liver, pancreas and adipose tissues were determined. Increases in blood glucose, cholesterol and triglycerides were evoked by STZ in both rat strains; however, the onset of hyperglycaemia was delayed in DPP4-deficient rats as compared with wild-type rats. By contrast, more severe dyslipidaemia was observed in DPP4-deficient rats than in wild-type rats after STZ treatment. Plasma DPP4 activity increased progressively with time after STZ treatment in wild-type rats. The kidney of wild-type rats showed decreased DPP4 activity with increased Dpp4 mRNA after STZ treatment. In addition, kidney weight, serum creatinine and excreted amounts of urinary protein, glucose and DPP4 enzyme were enhanced by STZ. DPP4-deficient rats showed increased serum creatinine in accordance with decreased creatinine clearance as compared with wild-type rats after STZ treatment. In conclusion, plasma DPP4 activity increased after STZ treatment, positively correlating to blood glucose. DPP4-deficient rats were resistant to developing diabetes, while susceptible to dyslipidaemia and reduction of glomerular filtration rate by STZ. DPP4 activation may be responsible for hyperglycaemia, lipid metabolism and preservation of renal function.
(キーワード): Animals / Cholesterol / Creatinine / Diabetes Mellitus, Experimental / Diabetic Nephropathies / Dipeptidyl Peptidase 4 / Disease Models, Animal / Disease Progression / Dyslipidemias / Gene Expression / Humans / Male / Rats / Rats, Transgenic / Streptozocin
(出版サイトへのリンク): ● Publication site (DOI): 10.1677/JOE-08-0424
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18931022; ● Search Scopus @ Elsevier (PMID): 18931022; ● Search Scopus @ Elsevier (DOI): 10.1677/JOE-08-0424

(DOI: 10.1677/JOE-08-0424, PubMed: 18931022) Hong-Tao Yan, Toshikatsu Shinka, Youichi Sato, Xin-Jun Yang, Gang Chen, Kozue Sakamoto, Keigo Kinoshita, Hiroyuki Aburatani and Yutaka Nakahori :
Overexpression of SOX15 Inhibits Proliferation of NT2/D1 Cells Derived from a Testicular Embryonal Cell Carcinoma,
Molecules and Cells, Vol.24, No.3, 323-328, 2007.

(要約): SOX (Sry-related HMG box) family proteins, which have an evolutionarily conserved DNA binding domain, have crucial roles in cell differentiation. However, their target genes remain enigmatic. Some members of the SOX family may have roles in regulation of cell proliferation. We established stable NT2/D1 cell lines overexpressing SOX15 (SOX15-NT2/D1), and a modified 3-(4,5-dime-thylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the SOX15-NT2/D1 cells exhibited significantly slower growth than the controls. Flow cytometry analysis revealed that an increased fraction of the SOX15-NT2/D1 cells were in G1-G0. In addition, a microarray analysis identified 26 genes that were up-regulated in the SOX15-NT2/D1 cells, but none that were down-regulated genes. Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle.
(キーワード): Carcinoma, Embryonal / Cell Line, Tumor / Cell Proliferation / DNA-Binding Proteins / Flow Cytometry / Gene Expression / High Mobility Group Proteins / Humans / Male / Oligonucleotide Array Sequence Analysis / RNA, Messenger / Reverse Transcriptase Polymerase Chain Reaction / SOX Transcription Factors / Testicular Neoplasms
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18182846; ● Search Scopus @ Elsevier (PMID): 18182846

(PubMed: 18182846) Takuro Nakano, Toshikatsu Shinka, Masako Sei, Youichi Sato, Mayumi Umeno, Kozue Sakamoto, Isoko Nomura and Yutaka Nakahori :
A/G heterozygote of the A-3826G polymorphism in the UCP-1 gene has higher BMI than A/A and G/G homozygote in young Japanese males,
The Journal of Medical Investigation : JMI, Vol.53, No.3,4, 218-222, 2006.

(要約): UCP-1 is suggested to have important roles for thermogenesis and energy expenditure. To elucidate whether the A-3826G polymorphism that is located in the 5' flanking region of the UCP-1 gene has roles in healthy young people, the polymorphism was genotyped among 251 young Japanese men whose mean age is 22.7 years old. We analyzed relationship between the A-3826G polymorphism and body mass index (BMI) or six biochemical parameters, serum concentration of total cholesterol (TC), high density lipoprotein (HDL) cholesterol, triglyceride (TG), asparatate aminotransferase (AST), alanine aminotransferase (ALT), fasting plasma glucose. The genotype frequencies were observed at the frequencies of 24.3% for AA, 48.2% for AG and 27.5% for GG, respectively. When BMI and the biochemical parameters were compared by ANOVA among individuals with each genotype, the statistical difference was observed only for BMI (P=0.016). Bonferroni's test demonstrated that the men with the AG genotype have higher BMI than those with the AA genotype (22.4+/-2.8 vs. 21.4+/-2.2) (P=0.04). The individuals with the AG genotype also showed trend to have higher BMI than those with the GG, although the difference was not statistically apparent (22.4+/-2.8 vs. 21.5+/-2.3) (P=0.07). Our results indicated that the young healthy Japanese men with the AG heterozygote showed higher BMI than those with other genotypes.
(キーワード): Adenine / Adult / Alleles / Asian Continental Ancestry Group / Body Composition / Body Mass Index / DNA / Genotype / Guanine / Heterozygote / Homozygote / Humans / Ion Channels / Japan / Male / Mitochondrial Proteins / Obesity / Polymorphism, Genetic
(徳島大学機関リポジトリ): ● Metadata: 110811
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.53.218
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16953057; ● Search Scopus @ Elsevier (PMID): 16953057; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.53.218

(徳島大学機関リポジトリ: 110811, DOI: 10.2152/jmi.53.218, PubMed: 16953057) Keigo Kinoshita, Toshikatsu Shinka, Youichi Sato, Hiroki Karahashi, Hiroe Kowa, Gang Chen, Mayumi Umeno, Kazunori Toida, Emi Kiyokage, Takuro Nakano, Susumu Ito and Yutaka Nakahori :
Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome,
The Journal of Medical Investigation : JMI, Vol.53, No.1,2, 117-122, 2006.

(要約): Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence [mHSFYL (Riken cDNA 4933413G11Rik)], which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA- or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.
(キーワード): Amino Acid Sequence / Animals / Base Sequence / Chromosomes, Human, Y / DNA, Complementary / DNA-Binding Proteins / Gene Expression / Heat-Shock Proteins / Humans / Male / Mice / Molecular Sequence Data / Oligospermia / Recombinant Proteins / Sequence Homology, Amino Acid / Species Specificity / Spermatids / Transcription Factors
(徳島大学機関リポジトリ): ● Metadata: 110796
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.53.117
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16538004; ● Search Scopus @ Elsevier (PMID): 16538004; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.53.117

(徳島大学機関リポジトリ: 110796, DOI: 10.2152/jmi.53.117, PubMed: 16538004) Mayumi Umeno, Toshikatsu Shinka, Youichi Sato, Xin-Jun Yang, Yoshinobu Baba, Teruaki Iwamoto and Yutaka Nakahori :
A rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR,
The Journal of Medical Investigation : JMI, Vol.53, No.1,2, 147-152, 2006.

(要約): Around 10% of males with idiopathic azoospermia or oligozoospermia, which are important causes of male infertility, have partial deletions on the long arm of the Y chromosome. To develop a rapid and accurate detection system for screening major Y deletions found in infertile men, we developed a multiplex PCR system that can simultaneously amplify five loci on the Y chromosome, SRY, AMELY, DBY, RBMY, DAZ and one locus on the X chromosome, AMELX. The size of the PCR products was designed to increase gradually from the distal Yp to the distal Yq. Our system could detect deletions of three major candidate regions for the azoospermic factor, AZFa, AZFb and AZFc on the Y chromosome together with sex. The gradual increase in the size of the PCR products was convenient for imaging the location of deletions on the Y chromosome. Moreover, the multiplex PCR system was combined with microchip-based electrophoresis, and the PCR products derived from each locus were separated within 4 min. Our system is useful for screening Y chromosomes bearing the structural anomalies including three major AZF deletions found among azoospermic or oligozoospermic males.
(キーワード): Base Sequence / Case-Control Studies / Chromosome Deletion / Chromosomes, Human, Y / DNA Primers / Electrophoresis / Genetic Loci / Humans / Male / Oligospermia / Polymerase Chain Reaction / Seminal Plasma Proteins
(徳島大学機関リポジトリ): ● Metadata: 110800
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.53.147
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16538008; ● Search Scopus @ Elsevier (PMID): 16538008; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.53.147

(徳島大学機関リポジトリ: 110800, DOI: 10.2152/jmi.53.147, PubMed: 16538008) Xin-Jun Yang, Toshikatsu Shinka, Shinka Nozawa, Hong-Tao Yan, Miki Yoshiike, Mayumi Umeno, Youichi Sato, Gang Chen, Teruaki Iwamoto and Yutaka Nakahori :
Survey of the two polymorphisms in DAZL, an autosomal candidate for the azoospermic factor, in Japanese infertile men and implications for male infertility,
Molecular Human Reproduction, Vol.11, No.7, 513-515, 2005.

(要約): The DAZL (DAZ-like) gene is suggested to be an ancestral gene of the DAZ (deleted in azoospermia) gene on the Y chromosome, which is a strong candidate for the azoospermic factor. Recently, it has been reported that the T54A (Thr54-->Ala) polymorphism in exon 3 of the DAZL gene is associated with spermatogenic failure in the Taiwanese population. In this study, to investigate whether this polymorphism is associated with spermatogenic failure in Japanese males, we analysed genomic DNA derived from 234 patients with azoospermia or oligozoospermia and 131 fertile controls. The T54A polymorphism was completely absent in both the patients and the controls. The T12A (Thr12-->Ala) polymorphism in exon 2 of the DAZL gene was found at a similar frequency in the patients and controls, 15.4% and 13.7%, respectively (P = 0.67). However, the frequency of T12A was higher for the azoospermic (20.5%) than oligozoospermic (9.6%) individuals in infertile men without DAZ deletions, although statistical difference was not so apparent (chi2 test: P = 0.037, OR = 2.413, 95% CI = 1.035-5.629; Yate's chi2 test: P = 0.058, OR = 2.319, 95% CI = 0.973-6.166). Our results show that the T54A polymorphism in DAZL has no major role in Japanese males with azoospermia or oligozoospermia. The distribution of the T54A polymorphism may be restricted to the narrow area including Taiwan.
(キーワード): azoospermic / DAZ / DAZL / oligozoospermia / Y chromosome
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/molehr/gah202
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16123080; ● Search Scopus @ Elsevier (PMID): 16123080; ● Search Scopus @ Elsevier (DOI): 10.1093/molehr/gah202

(DOI: 10.1093/molehr/gah202, PubMed: 16123080) Hong-Tao Yan, Toshikatsu Shinka, Keigo Kinoshita, Youichi Sato, Mayumi Umeno, Gang Chen, Keiko Tsuji, Yukiko Unemi, Xin-Jun Yang, Teruaki Iwamoto and Yutaka Nakahori :
Molecular analysis of TBL1Y, a Y-linked homologue of TBL1X related with X-linked late-onset sensorineural deafness,
Journal of Human Genetics, Vol.50, No.4, 175-181, 2005.

(要約): Recent progress in sequencing the human Y chromosome has unveiled a series of X-Y homologous genes. In the present study, we focused on Transducin beta-like 1Y (TBL1Y), which is a Y-linked homologue of TBL1X that is related with X-linked late-onset sensorineural deafness. Recently, it has been shown that TBLR1, another homologue whose gene resides on chromosome 3, and TBL1X act as a corepressor/coactivator exchanger for several nuclear receptors and transcription factors. However, the expression pattern and function of TBL1Y remain unknown. The RT-PCR analysis of the TBL1 family revealed that TBL1Y was expressed in all 13 tissues examined but not in leukocytes. Among the cell lines tested, however, it was only expressed in NT2/D1 cells and in lymphoblasts transformed with Epstein Barr (EB) virus. To compare the functions of the TBL1 family, we generated a series of expression plasmids for GAL4DBD-fused proteins of the TBL1 family. We carried out dual luciferase assays using these plasmids in combination with a plasmid having a luciferase reporter gene harboring 5xGAL4 binding sites. Unlike the other constructs, GAL4DBD-fused TBL1Y did not repress the promoter activity. Moreover, we found three novel polymorphisms in the TBL1Y gene, IVS7+9G>A, G268C, and IVS7+1G>C, which is presumed to cause splicing error. These polymorphisms are found in males within Y-haplogroup O3 (XO3e), which is defined as the Y-haplogroup O3 excluding O3e, a branch of O3. The results show that TBL1Y differs from other members of the TBL1 family in expression and function, suggesting other roles in maleness.
(キーワード): Age of Onset / Amino Acid Sequence / Chromosomes, Human, X / Chromosomes, Human, Y / Female / Genetic Linkage / Hearing Loss, Sensorineural / Humans / Luciferases / Male / Molecular Sequence Data / Polymorphism, Genetic / Promoter Regions, Genetic / Recombinant Fusion Proteins / Sequence Homology, Amino Acid / Transducin
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10038-005-0237-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15834507; ● Search Scopus @ Elsevier (PMID): 15834507; ● Search Scopus @ Elsevier (DOI): 10.1007/s10038-005-0237-9

(DOI: 10.1007/s10038-005-0237-9, PubMed: 15834507) Hirofumi Shibata, Kyoko Kondo, Ryo Katsuyama, Kazuyoshi Kawazoe, Youichi Sato, Kotaro Murakami, Yoshihisa Takaishi, Naokatu Arakaki and Tomihiko Higuti :
Alkyl Gallates, Intensifiers of ß-Lactam Susceptibility in Methicillin-Resistant Staphylococcus aureus,
Antimicrobial Agents and Chemotherapy, Vol.49, No.2, 549-555, 2005.

(要約): We found that ethyl gallate purified from a dried pod of tara (Caesalpinia spinosa) intensified beta-lactam susceptibility in methicillin-resistant and methicillin-sensitive strains of Staphylococcus aureus (MRSA and MSSA strains, respectively). This compound and several known alkyl gallates were tested with MRSA and MSSA strains to gain new insights into their structural functions in relation to antimicrobial and beta-lactam susceptibility-intensifying activities. The maximum activity of alkyl gallates against MRSA and MSSA strains occurred at 1-nonyl and 1-decyl gallate, with an MIC at which 90% of the isolates tested were inhibited of 15.6 microg/ml. At concentrations lower than the MIC, alkyl gallates synergistically elevated the susceptibility of MRSA and MSSA strains to beta-lactam antibiotics. Such a synergistic activity of the alkyl gallates appears to be specific for beta-lactam antibiotics, because no significant changes were observed in the MICs of other classes of antibiotics examined in this study. The length of the alkyl chain was also associated with the modifying activity of the alkyl gallates, and the optimum length was C5 to C6. The present work clearly demonstrates that the length of the alkyl chain has a key role in the elevation of susceptibility to beta-lactam antibiotics.
(キーワード): Anti-Bacterial Agents / Drug Synergism / Gallic Acid / Magnetic Resonance Spectroscopy / Methicillin Resistance / Microbial Sensitivity Tests / Plant Extracts / Plants, Medicinal / Spectrometry, Mass, Electrospray Ionization / Staphylococcus aureus / beta-Lactams
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AAC.49.2.549-555.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15673731; ● Search Scopus @ Elsevier (PMID): 15673731; ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.49.2.549-555.2005

(DOI: 10.1128/AAC.49.2.549-555.2005, PubMed: 15673731) 佐藤陽一, 新家利一, 陳剛, 閻洪涛, 坂本梢, 楊新軍, 梅野真由美, 中堀豊 :
性分化と精子形成機構に対するプロテオミクスからのアプローチ,
四国医学雑誌, Vol.60, No.5-6, 168-171, 2004年.

(要約): SRY(sex determining region on the Y chromosome)was a gene isolated as the testis determiningfactor on the Y chromosome in1990．The gene consisted of single exon and encodes a204aminoacid protein. Since SRY protein has a HMG domain, which functions as DNA binding domain, it hasbeen considered to be a transcription factor. Direct target of SRY, however, have not yet identifiedfor14years.In this study, we have performed a proteomics approach to analyze the function of SRY. Twodimensionalgel electrophoresis after over expression of SRY showed considerable down regulationin many proteins. Among the proteins, mitochondrial 60‐kDa heat shock protein(HSP60)andprobable protein disulfide isomerase(ER60)remarkably decreased. Since they were reported tobe associated with cell proliferation and differentiation, we investigated the effects of SRY on cellcycle profiles. It was shown that the over‐expression of SRY negatively regulated the cell growthwith significant S or G2/M arrest of cell cycle.
(キーワード): SRY / sex determination / Y chromosome / poteomics
(徳島大学機関リポジトリ): ● Metadata: 101801
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050282812440620800

(徳島大学機関リポジトリ: 101801, CiNii: 1050282812440620800) Youichi Sato, Hirofumi Shibata, Tsutomu Arai, Akira Yamamoto, Yousuke Okimura, Naokatu Arakaki and Tomihiko Higuti :
Variation in synergistic activity by flavone and its related compounds on the increased susceptibility of various strains of methicillin-resistant Staphylococcus aureus to β-lactam antibiotics,
International Journal of Antimicrobial Agents, Vol.24, No.3, 226-233, 2004.

(要約): We found that some flavonoids had a weak antibacterial effect on methicillin-resistant Staphylococcus aureus (MRSA), but that at sub-MIC concentrations they greatly increased the susceptibility of these strains to beta-lactam antibiotics. Flavone showed diverse synergistic effects on the susceptibility of MRSA to beta-lactam antibiotics. The variation of the synergistic effects of the flavones to increase the susceptibility of strains of MRSA to beta-lactam antibiotics coincided with their varying effects on growth-inhibition of these strains. Based on these findings, we have proposed a model for the mechanisms of high resistance of MRSA to beta-lactams and the massive reduction in the beta-lactams MIC caused by flavones.
(キーワード): Anti-Bacterial Agents / Bacterial Proteins / Cell Division / Drug Synergism / Flavones / Flavonoids / Gene Expression / Methicillin Resistance / Microbial Sensitivity Tests / RNA, Bacterial / RNA, Messenger / Staphylococcus aureus / beta-Lactams
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijantimicag.2004.02.028
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15325425; ● Search Scopus @ Elsevier (PMID): 15325425; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijantimicag.2004.02.028

(DOI: 10.1016/j.ijantimicag.2004.02.028, PubMed: 15325425) Youichi Sato, Hirofumi Shibata, Naokatu Arakaki and Tomihiko Higuti :
6,7-dihydroxyflavone dramatically intensifies the susceptibility of methicillin-resistant or -sensitive Staphylococcus aureus to beta-lactams.,
Antimicrobial Agents and Chemotherapy, Vol.48, No.4, 1357-1360, 2004.

(要約): We have demonstrated that 6,7-dihydroxyflavone by itself has only a weak antibacterial effect on methicillin-resistant Staphylococcus aureus (MRSA) but that at concentrations less than MIC it synergistically elevates the susceptibility of clinically isolated MRSA and methicillin-sensitive S. aureus strains to beta-lactam antibiotics from 8- to 32,800-fold.
(キーワード): Anti-Bacterial Agents / Anti-Infective Agents / Colony Count, Microbial / Drug Synergism / Flavones / Flavonoids / Humans / Methicillin Resistance / Microbial Sensitivity Tests / Staphylococcal Infections / Staphylococcus aureus / Time Factors / beta-Lactams
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AAC.48.4.1357-1360.2004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15047543; ● Search Scopus @ Elsevier (PMID): 15047543; ● Search Scopus @ Elsevier (DOI): 10.1128/AAC.48.4.1357-1360.2004

(DOI: 10.1128/AAC.48.4.1357-1360.2004, PubMed: 15047543) Hirofumi Shibata, Chikako Shirakata, Hiromi Kawasaki, Youichi Sato, Tomomi Kuwahara, Yoshinari Ohnishi, Naokatu Arakaki and Tomihiko Higuti :
Flavone markedly affects phenotypic expression of beta-lactam resistance in methicillin-resistant Staphylococcus aureus strains isolated clinically.,
Biological & Pharmaceutical Bulletin, Vol.26, No.10, 1478-1483, 2003.

(要約): Flavone and its derivatives had very weak antibacterial effects on niethicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive S. aureus, but dramatically intensified MRSA's susceptibility to β-lactams. We named these compounds "ILSMR (intensifier of β-lactam-susceptibility in MRSA)." We also found discrepancies among MRSA strains in their responses to flavone; some strains showed phenotypic susceptibility to methicillin while others showed phenotypic resistance to it. To understand the mechanism underlying this discrepancy, we characterized 20 MRSA strains in detail, analyzed their conventional and molecular typings, and examined each strain's resistance to β-lactams, with COL serving as a reference. Neither SCCmec typing nor coagulase typing explained the diverse effects of flavone on the β-lactam MICs of these strains. Likewise, changes in pulsed-field gel electrophoresis (PFGE) type were not associated with the profiles of ILSMR effects. However, the present observations suggest that the ILSMR effects on MRSA is strain-specific, and that this effect depends on an as-yet unknown mechanism that is essential for the expression of the phenotype conferring β-lactam resistance to MRSA strains, independently of an interaction with the mecA-encoded penicillin-binding protein 2a or with the β-lactamase.
(キーワード): methicillin-resistant Staphvlococcus aureus (MRSA) / flavone / β-lactam-resistance / SCCmec / population analysis / minimum inhibitory concentration (MIC)
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.26.1478
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14519958; ● CiNii @ 国立情報学研究所 (CRID): 1390282679604409856; ● Search Scopus @ Elsevier (PMID): 14519958; ● Search Scopus @ Elsevier (DOI): 10.1248/bpb.26.1478

(DOI: 10.1248/bpb.26.1478, PubMed: 14519958, CiNii: 1390282679604409856) Youichi Sato, Shiho Suzaki, Takako Nishikawa, Masaru Kihara, Hirofumi Shibata and Tomihiko Higuti :
Phytochemical flavones isolated from Scutellaria barbata and antibacterial activity against methicillin-resistant Staphylococcus aureus.,
Journal of Ethnopharmacology, Vol.72, No.3, 483-488, 2000.

(要約): A crude extract prepared from Scutellaria barbata D. Don (Lamiaceae) was analyzed in the effort to discover antibacterial compounds against high-level strains of methicillin-resistant Staphylococcus aureus (MRSA). Apigenin and luteolin were isolated from the plant as active constituents against the bacteria. These flavonoid congeners were selectively toxic to S. aureus, including the MRSA and methicillin-sensitive S. aureus strains.
(キーワード): Scutellaria barbata / Antibacterial activity / Apigenin / Luteolin / Methicillin-resistant Staphylococcus aureus
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0378-8741(00)00265-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10996290; ● Search Scopus @ Elsevier (PMID): 10996290; ● Search Scopus @ Elsevier (DOI): 10.1016/S0378-8741(00)00265-8

(DOI: 10.1016/S0378-8741(00)00265-8, PubMed: 10996290) Youichi Sato, H Oketani, T Yamada, K Singyouchi, T Ohtsubo, Masaru Kihara, Hirofumi Shibata and Tomihiko Higuti :
A Xanthanolide with Potent Antibacterial Activity against Methicillin-resistant Staphylococcus aureus.,
The Journal of Pharmacy and Pharmacology, Vol.49, 1042-1044, 1997. Youichi Sato, H Oketani, K Singyouchi, T Ohtubo, Masaru Kihara, Hirofumi Shibata and Tomihiko Higuti :
Extraction and Purification of Effective Antimicrobial Constituents of Terminalia chebula RETS. against Methicillin-Resistant Staphylococcus aureus.,
Biological & Pharmaceutical Bulletin, Vol.20, 401-404, 1997.

MISC

佐藤陽一 :
男性不妊症関連遺伝子の精子形成メカニズム,
アグリバイオ, Vol.3, No.14, 81-84, 2019年. 佐藤陽一 :
男性不妊症関連遺伝子の精子形成メカニズム,
細胞, Vol.51, No.5, 38-40, 2019年.

(キーワード): 男性不妊症 / 精子形成 / 遺伝子 / SNP
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520291853569993344

(CiNii: 1520291853569993344) Jun Yamamoto, Miku Kita, Akira Shigenaga, Youichi Sato, Aiko Yamauchi and Akira Otaka :
Development of thiol-responsive traceable linker for efficient enrichment and selective labeling of target proteins,
Peptide Science 2013, 205-206, 2014. 佐藤陽一, 中堀豊 :
日本人類学会遺伝分科会平成22年度公開シンポジウム「日本人の成り立ちについての新たな考察―遺伝と形態から―Y染色体からみた日本人の成り立ち,
Anthropological Science (Japanese Series), Vol.118, No.2, 115-118, 2010年.

(出版サイトへのリンク): ● Publication site (DOI): 10.1537/asj.118.115
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1537/asj.118.115

(DOI: 10.1537/asj.118.115)

総説・解説

佐藤陽一 :
肥満・糖尿病研究の紹介(4) ∼DPP-4活性と1型糖尿病∼,
県薬だより-情報とくしま-, No.78, 25-27, 2013年9月. 佐藤陽一 :
肥満・糖尿病研究の紹介(3)∼DPP-4活性と2型糖尿病∼,
県薬だより-情報とくしま-, No.77, 17-19, 2013年6月. 傳田将也, 山本純, 佐藤浩平, 坂本健, 重永章, 佐藤陽一, 吉村好之, 山内あい子, 大髙章 :
新規タンパク質選択的ラベル化試薬''SEAL-tag''の開発研究,
ケミカルバイオロジー, Vol.6, 6-9, 2013年6月. 佐藤陽一 :
肥満・糖尿病研究の紹介(2)∼DPP-4活性と肥満∼,
県薬だより-情報とくしま-, No.76, 7-9, 2013年3月. 佐藤陽一 :
肥満・糖尿病研究の紹介(1),
県薬だより-情報とくしま-, No.75, 36-39, 2012年12月.

講演・発表

Youichi Sato, Fukunaga Chika, Kojo Kosuke, Uchida Masahiro, Tsuchiya Haruki, Yamasaki Kazumitu and Iwamoto Teruaki :
Elucidation of the causative gene of non-obstructive azoospermia by whole-exome sequencing,
ASHG2019, Houston, Oct. 2019. Youichi Sato, Atsushi Tajima, Kogusuri Suzu, Fuji Aki, Sato Takehiro, Issei Imoto and Iwamoto Teruaki :
Identification of genetic loci related to circulating reproductive hormone levels by GWAS in Japanese men,
ASHG2018, San Diego, Oct. 2018. Soushi Imani, Youichi Sato, Tatsuya Shimozawa, Teruaki Iwamoto and Aiko Yamauchi :
Association analyses between copy numbers of genes in the azoospermia factor c (AZFc) region on the Y chromosome and male infertility,
The 13th International Congress of Human genetics, Kyoto, Apr. 2016. Youichi Sato, Atsushi Tajima, Katsurayama Motoki, Issei Imoto, Aiko Yamauchi and Iwamoto Teruaki :
A replication study of four candidate loci for sex hormone levels previously identified by genome-wide association studies,
The 13th International Congress of Human Genetics, Kyoto, Apr. 2016. Masaya Denda, Takuya Morisaki, Jun Yamamoto, Kohei Sato, Tsubasa Inokuma, Youichi Sato, Aiko Yamauchi, Akira Shigenaga and Akira Otaka :
In Cell Labeling of Target Proteins using ''SEAL-tag'',
American Peptide Symposium 2015, Florida, Jun. 2015. Hitoshi Sumi, Youichi Sato and Shinji Harihara :
The two Japanese ancestral groups, Jomon and Yayoi peoples From the perspective of mitochondrial DNA and Y chromosomal DNA polymorphisms-,
Forum for Anthropology and Paleopathology in East Asia, Tokyo, Nov. 2014. Hitoshi Sumi, Youichi Sato and Shinji Harihara :
Genetic Analysis of Hida Population in Central Japanese - From the Viewpoint of Polymorphisms of Mitochondrial DNA and Y chromosome-,
International Symposium on Mitochondria 2013, Tokyo, Nov. 2013. Jun Yamamoto, Miku Kita, Akira Shigenaga, Youichi Sato, Aiko Yamauchi and Akira Otaka :
Development of thiol-responsive traceable linker for efficient enrichment and selective labeling of target proteins,
4th Asia-Pacific International Peptide Symposium, 50th Japanese Peptide Symposium, Suita, Nov. 2013. Jun Yamamoto, Miku Kita, Akira Shigenaga, Youichi Sato, Aiko Yamauchi and Akira Otaka :
Application of thiol-responsive amino acid to traceable linker for purification and selective labeling of target protein,
23rd American Peptide Symposium, Hawai'i, Jun. 2013. Youichi Sato, Iwamoto Teruaki, Toshikatsu Shinka, Aiko Yamauchi and Yutaka Nakahori :
Seasonal variation on the reproductive characteristics and sperm concentration of Japanese males,
7th Copenhagen Workshop on Endocrine Disrupters, Copenhagen, May 2013. Youichi Sato, Toshikatsu Shinka, Aiko Yamauchi and Yutaka Nakahori :
Overview of the genetic variations in the Y chromosome in the Japanese population,
The American Society of Human Genetics, 62nd Annual Meeting, San Francisco, Nov. 2012. Masaya Denda, Jun Yamamoto, Kohei Sato, Ken Sakamoto, Akira Shigenaga, Youichi Sato, Aiko Yamauchi and Akira Otaka :
Development of a novel chemical probe that enables selective labeling of proteins,
The 1st International Symposium on Chemical Biology of Natural Products: Target ID and Regulation of Bioactivity, Kyoto, Oct. 2012. Yukari Shono, Mami Adachi, Kumiko Sakamoto, Youichi Sato and Aiko Yamauchi :
Prediction of drug-induced hepatotoxicity in human using machine learning,
The Second Decennial Meeting Between SeoulNational University and The University of Tokushima, Awaji, Dec. 2010. Youichi Sato, Toshikatsu Shinka, Kozue Sakamoto and Yutaka Nakahori :
The male-determining gene SRY is a hybrid of DGCR8 and SOX3, and is regulated by the transcription factor CP2,
The American Society of Human Genetics, 58th Annual Meeting, Philadelphia, Nov. 2008. 佐藤陽一 :
AKR1C3をターゲットとした化合物の精子形成への関与,
第69回日本生殖医学会学術講演会, 2024年11月. 中村麻理奈, 長﨑裕加, 佐藤陽一 :
Association of CYP gene polymorphisms with adverse events and blood levels of vancomycin administration,
日本人類遺伝学会第69回大会, 2024年10月. 新居謙司郎, 多田篤史, 中川雄介, 長﨑裕加, 佐藤陽一 :
Evolution of the Japanese Y chromosome in the context of haplogroups and AZFc region deletion patterns,
日本人類遺伝学会第69回大会, 2024年10月. 菊地康友, 古城公佑, 沼畑大介, 内田将央, 山崎一恭, 岩本晃明, 長﨑裕加, 佐藤陽一 :
Development of a predictive model for sperm retrieval by micro-TESE using genomic information,
日本人類遺伝学会第69回大会, 2024年10月. 西田玖二子, 古城公佑, 沼畑大介, 内田将央, 山崎一恭, 岩本晃明, 長﨑裕加, 佐藤陽一 :
Investigation of genetic causes by exome sequencing in families with non-obstructive azoospermia,
日本人類遺伝学会第69回大会, 2024年10月. 髙見真理子, 黒部匡広, 土屋春樹, 古城公佑, 内田将央, 山崎一恭, 岩本晃明, 佐藤陽一 :
非閉塞性無精子症患者43名の全エクソームシーケンス,
第68回日本生殖医学会学術講演会, 2023年11月. 入江彩香, 佐藤陽一 :
シタラビンによる副作用発現とクローン造血関連遺伝子DNMT3Aとの関連解析,
第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2023年10月. 若野奏海, 佐藤陽一 :
ゲノムワイド関連解析による精索静脈瘤関連遺伝子の同定とin silico解析,
第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2023年10月. 藤田彩花, 田嘉祥, 笠原朱莉, 佐藤陽一 :
AKR1C3をターゲットとした男性不妊症治療薬の開発研究,
第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2023年10月. 原田真優, 佐藤陽一 :
AKR1C3をターゲットとした肝細胞癌治療のための新規抗腫瘍薬の探索,
第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2023年10月. 組橋由記, 佐藤陽一 :
大腸癌患者におけるCapOX療法による機械学習を用いた副作用発現予測モデルの構築,
第62回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2023年10月. 井上誠, 佐藤陽一 :
日本人のY染色体ハプログループ解析と地域頻度,
日本人類遺伝学会第67回大会, 2022年12月. 伊東佑星, 小西麻実, 山本清威, 佐藤陽一 :
ヒト精子運動率関連遺伝子ERBB4をターゲットとした化合物の探索と精子運動率の検討,
第95回日本生化学会大会, 2022年11月. 宮田茉奈, 佐藤陽一 :
Inhibin B関連遺伝子Lrriq1の雄生殖能力に与える影響についての検討,
第95回日本生化学会大会, 2022年11月. 笠原朱莉, 山本清威, 佐藤陽一 :
GWASによる精子濃度関連遺伝子座の同定とゲノム創薬研究,
第95回日本生化学会大会, 2022年11月. 山田博貴, 大森理央, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
ゲノムワイド関連解析によるビンクリスチン副作用発現関連遺伝子の同定と機械学習を用いた副作用発現予測モデルの構築,
第60回日本薬学会中国四国支部学術大会, 2021年11月. 小西麻実, 佐藤陽一 :
精子運動率関連遺伝子ERBB4をターゲットとした男性不妊症に対するゲノム創薬,
第60回日本薬学会中国四国支部学術大会, 2021年11月. 中川雄介, 多田篤史, 佐藤陽一 :
日本人男性を対象としたAZFc領域の遺伝子欠失と精子の質との関連解析,
第60回日本薬学会中国四国支部学術大会, 2021年11月. 三浦遥香, 土屋春樹, 松本吉隆, 古城公佑, 内田将央, 岩本晃明, 佐藤陽一 :
ゲノム解析による顕微鏡下精巣内精子採取術の精子回収予測モデルの構築,
日本アンドロロジー学会第40回学術大会, 2021年6月. 福富茉由, 上殿千晴, 佐藤陽一 :
Lrriq1ノックアウトマウスのInhibin B及び生殖能力に与える影響,
日本アンドロロジー学会第40回学術大会, 2021年6月. 佐藤陽一, 土屋春樹, 古城公佑, 内田将央, 山崎一恭, 岩本晃明 :
遺伝情報の機械学習を用いた顕微鏡下精巣内精子採取術における精子回収予測の検討,
日本アンドロロジー学会第39回学術大会, 2021年1月. 武井みのり, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
機械学習による遺伝情報を用いた抗がん剤投与の味覚障害発現予測モデルの構築,
第59回中国四国支部学術大会, 2020年12月. 遠藤優香, 田島穂澄, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
ゲノム情報を用いた機械学習によるシタラビン投与後の副作用発現予測モデルの構築,
第59回中国四国支部学術大会, 2020年12月. 井上誠, 佐藤陽一 :
現代日本人のY染色体ハプログループ解析,
日本人類遺伝学会第65回大会, 2020年11月. 多田篤史, 足立奈生子, 井上誠, 佐藤陽一 :
日本人男性のY染色体ハプログループとAZF領域欠失パターンとの関連性,
日本人類遺伝学会第65回大会, 2020年11月. 上殿千晴, 藤井亜紀, 田嶋敦, 佐藤丈寛, 井本逸勢, 佐藤陽一 :
ゲノムワイド関連解析による血中inhibin B値関連遺伝子の同定と遺伝子改変マウスの解析,
日本人類遺伝学会第65回大会, 2020年11月. 国村彩香, 福永千香, 古城公佑, 内田将央, 土屋春樹, 松本吉隆, 山崎一恭, 岩本晃明, 佐藤陽一 :
非閉塞性無精子症に対する全エクソーム解析,
日本人類遺伝学会第65回大会, 2020年11月. 多田篤史, 足立奈生子, 井上誠, 佐藤陽一 :
Y染色体ハプログループとAZF領域欠失パターンとの関連性から見た日本人のY染色体進化,
第74回日本人類学会大会, 2020年10月. 遠藤優香, 田島穂澄, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
機械学習を用いたゲノム情報によるシタラビン投与の副作用発現予測モデルの構築,
日本薬学会第140年会, 2020年3月. 武井みのり, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
遺伝情報を用いた機械学習による抗がん剤投与の味覚障害発現予測モデルの構築,
日本薬学会第140年会, 2020年3月. 遠藤優香, 田島穂澄, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
機械学習を用いたゲノム情報によるシタラビン投与の副作用発現予測モデルの構築,
日本薬学会年会要旨集, 26M-am07S, 2020年3月.

(キーワード): Cytarabine(毒性・副作用) / 皮膚疾患(化学的誘発) / リスク評価 / 機械学習 / ヒト

佐藤陽一 :
ゲノムワイド関連解析によるヒト精子形成機構に関わる遺伝子の探索と機能解析,
第42回日本分子生物学会年会, 2019年12月. 大谷知子, 賀勢泰子, 國友一史, 下岡和美, 川添和義, 佐藤陽一, 山内あい子 :
寝たきり高齢患者の腎機能を評価するための血清クレアチニン値の補正方法に関する検討,
第13回日本腎臓病薬物療法学会学術集会・総会, 2019年11月. 濱口惠寛, 山崎一恭, 岩本晃明, 佐藤陽一 :
GWASによる精索静脈瘤関連遺伝子座の探索,
第64回日本生殖医学会学術講演会, 2019年11月. 藤井亜紀, 山崎一恭, 岩本晃明, 佐藤陽一 :
ゲノムワイド関連解析によるInhibin B値関連遺伝子座の同定と遺伝子改変マウス作製によるInhibin B産生に関する検討,
第64回日本生殖医学会学術講演会, 2019年11月. 佐藤陽一, 古城公佑, 内田将央, 土屋春樹, 山崎一恭, 岩本晃明 :
個別化医療に向けた非閉塞性無精子症患者の次世代シーケンス解析,
第64回日本生殖医学会学術講演会, 2019年11月. 道上巧基, 福田達也, 佐藤陽一, 小暮健太朗 :
微弱電流処理による精巣への薬物送達,
遺伝子・デリバリー研究会第19回夏期セミナー, 2019年9月. 道上巧基, 福田達也, 田中保, 佐藤陽一, 小暮健太朗 :
男性不妊症治療を目指した微弱電流処理による精巣への非侵襲的薬物送達技術の開発,
第35回日本DDS学会学術集会, 2019年7月. 福永千香, 古城公佑, 内田将央, 土屋春樹, 山崎一恭, 岩本晃明, 佐藤陽一 :
次世代シーケンス解析は非閉塞性無精子症の原因解明に有効か?,
日本アンドロロジー学会第38回学術大会, 2019年6月. 野崎瑞貴, 上殿千晴, 古城公佑, 内田将央, 土屋春樹, 山崎一恭, 岩本晃明, 佐藤陽一 :
家系を対象とした次世代シーケンス解析による新規男性不妊症原因遺伝子の同定と遺伝子改変マウス作製による精子形成に関する検討,
日本アンドロロジー学会第38回学術大会, 2019年6月. 谷垣雄都, 山口裕大, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
ゲノムワイド関連解析を用いたテイコプラニン・バンコマイシン投与による皮疹発現および血中濃度関連遺伝子座の同定,
日本薬学会第139年会, 2019年3月. 大森理央, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
ゲノムワイド関連解析を用いたビンクリスチンによる末梢神経障害発現関連遺伝子の同定,
第57回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2018年11月. 山口裕大, 谷垣雄都, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 佐藤陽一 :
CYP遺伝子多型とバンコマイシンの副作用発現及び血中濃度との関連解析,
第57回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2018年11月. 小薬鈴, 藤井亜紀, 田嶋敦, 佐藤丈寛, 井本逸勢, 岩本晃明, 佐藤陽一 :
日本人を対象としたGWASによる生殖ホルモン値と関連する遺伝子座の同定,
第91回日本生化学会大会, 2018年9月. 木口美沙妃, 田嶋敦, 佐藤丈寛, 井本逸勢, 岩本晃明, 佐藤陽一 :
WASによる精液パラメータ及び精巣サイズ関連遺伝子座の同定,
第91回日本生化学会大会, 2018年9月. 佐藤陽一, 福永千香, 古城公佑, 内田将央, 土屋春樹, 岩本晃明 :
非閉塞性無精子症患者5例を対象とした次世代シーケンス解析,
第63回日本生殖医学会学術講演会・総会, 2018年9月. 小薬鈴, 佐藤陽一, 田嶋敦, 佐藤丈寛, 井本逸勢, 岩本晃明, 山内あい子 :
GWASによる血中inhibin Bレベル関連遺伝子座の探索,
ConBio2017, 2017年12月. 木口美沙妃, 佐藤陽一, 田嶋敦, 佐藤丈寛, 井本逸勢, 岩本晃明, 山内あい子 :
GWASによる精子濃度の関連遺伝子座の探索,
ConBio2017, 2017年12月. 長谷川知世, 佐藤陽一, 岩本晃明, 山内あい子 :
DPF3，TUSC1およびIZUMO3遺伝子上のSNPと男性不妊症との関連解析,
ConBio2017, 2017年12月. 佐藤陽一, 古城公佑, 内田将央, 土屋春樹, 野崎瑞貴, 岩本晃明, 山内あい子 :
家系を対象とした次世代シーケンス解析による新規無精子症原因遺伝子同定の試み,
第62回日本生殖医学会学術講演会, 2017年11月. 福永千香, 佐藤陽一, 岩本晃明, 山内あい子 :
日本人を対象としたメチレンテトラヒドロ葉酸還元酵素遺伝子C677T多型と男性不妊症との関連解析,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. 谷垣雄都, 佐藤陽一, 山口裕大, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 山内あい子 :
テイコプラニン投与による副作用発現および血中濃度とCYP遺伝子多型との関連解析,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. 田島穂澄, 佐藤陽一, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 山内あい子 :
シタラビン投与による副作用発現と代謝経路関連遺伝子多型の関連解析,
第56回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2017年10月. Matsuoka Koki, AHMAD AMMAR GHAIBEH, Omura Shiro, Youichi Sato, Hiroki Moriguchi and Aiko Yamauchi :
Integrated analysis for drug toxicities in human using multi-label classification.,
CBI学会2017年大会, Oct. 2017. 宗野真和, 松岡一郎, 牧純, 阿部真治, 佐藤陽一, 飯原なおみ, 宮澤宏, 通元夫, 桐野豊 :
海外の薬学教育(3)―伝統を携え改革に取り組む，イギリス・フランス・ドイツの薬学教育―,
第2回日本薬学教育学会, 2017年9月. 大森理央, 佐藤陽一, 木口美沙妃, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 山内あい子 :
ビンクリスチンによる副作用発現とMDR1遺伝子多型との関連性,
第20回日本医薬品情報学会総会・学術大会, 2017年7月. 山口裕大, 佐藤陽一, 谷垣雄都, 岡田直人, 中村信元, 賀川久美子, 藤井志朗, 三木浩和, 石澤啓介, 安倍正博, 山内あい子 :
バンコマイシン投与による副作用発現及び血中濃度とCYP遺伝子多型との関連解析,
第20回日本医薬品情報学会総会・学術大会, 2017年7月. 岡島八千代, 佐藤陽一, 山内あい子 :
日本人の食品品目別一日喫食量の将来予測,
日本食品化学学会第23回総会・学術大会, 2017年6月. 山本清威, 水口博之, 渡嘉敷夏海, 小林誠, 佐藤陽一, 藤野裕道, 福井裕行, 山内あい子 :
膵α細胞からのグルカゴン分泌に関与するPKCアイソザイムの同定,
日本薬学会第137年会, 2017年3月. 松田実紗, 岡田直人, 佐藤陽一, 山内あい子, 川添和義 :
DPP4遺伝子多型と肥満との関連解析,
第55回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2016年11月. 山西愛実, 岡田直人, 佐藤陽一, 山内あい子, 川添和義 :
インクレチンホルモン及びその受容体の遺伝子多型と肥満との関連解析,
第55回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2016年11月. 大村士朗, 松岡恒輝, 佐藤陽一, 山内あい子, ガイベアハマドアンマール, 森口博基 :
Support Vector Machine及び決定木による化合物のヒト発がん性予測モデルの構築,
第55回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2016年11月. 内田将央, 佐藤陽一, 古城公佑, 岩本晃明 :
AZFc部分欠失gr/gr欠失例の臨床像の検討,
第61回日本生殖医学会学術講演会, 2016年11月. 大谷知子, 賀勢泰子, 國友一史, 下岡和美, 山本浩子, 川添和義, 佐藤陽一, 山内あい子 :
寝たきり高齢者における腎機能評価に影響を与える因子の検討,
第24回日本慢性期医療学会, 2016年10月. Matsuoka Koki, AHMAD AMMAR GHAIBEH, Omura Shiro, Youichi Sato, Hiroki Moriguchi and Aiko Yamauchi :
"Prediction of chemical-induced developmental and reproductive toxicity in human using the machine learning",
CBI学会2016年大会, Oct. 2016. 山本清威, 水口博之, 小林誠, 佐藤陽一, 福井裕行, 山内あい子 :
膵α細胞からのグルカゴン分泌に対するquercetinの効果,
日本薬学会第136年会, 2016年3月. 今仁聡志, 佐藤陽一, 下澤辰也, 岩本晃明, 山内あい子 :
Y染色体上azoospermia factor c (AZFc) 領域内に存在する遺伝子のコピー数と男性不妊症との関連解析,
第38回日本分子生物学年会・第88回日本生化学会大会合同大会, 2015年12月. 松岡恒輝, 佐藤陽一, 大髙章, 山内あい子 :
薬物の胎児毒性に関する機械学習による予測と化学構造特性の解析,
第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2015年11月. 辻弥生, 佐藤陽一, 川添和義, 山内あい子, 石澤啓介 :
NSAIDsの種類及び服薬期間，頻度と発癌リスクに関するメタ解析,
第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2015年11月. 山本清威, 水口博之, 小林誠, 佐藤陽一, 福井裕行, 山内あい子 :
PKCδシグナル抑制化合物によるグルカゴン分泌抑制効果,
第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2015年10月. 葛山元貴, 佐藤陽一, 田嶋敦, 井本逸勢, 岩本晃明, 山内あい子 :
性ホルモン関連遺伝子座3 SNPsの日本人男性を対象としたreplication解析,
第54回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2015年10月. 大谷知子, 賀勢泰子, 國友一史, 川添和義, 佐藤陽一, 山内あい子 :
寝たきり高齢者における腎機能評価 ∼Scr補正の検証∼,
第9回日本腎臓病薬物療法学会学術大会2015, 2015年10月. 傳田将也, 森崎巧也, 猪熊翼, 佐藤陽一, 山内あい子, 重永章, 大髙章 :
N-Sアシル基転移反応を基盤としたin cellタンパク質ラベル化法の開発,
第47回若手ペプチド夏の勉強会, 2015年8月. 傳田将也, 森崎巧也, 猪熊翼, 佐藤陽一, 山内あい子, 重永章, 大髙章 :
N-Sアシル基転移反応を利用した新規ラベル化試薬の開発,
創薬懇話会2015 in 徳島, 2015年7月. 松岡恒輝, 稲山義高, 大村士朗, 佐藤陽一, 山内あい子 :
ヒト胎児に対する薬物の安全性を機械学習により予測する,
第18回日本医薬品情報学会総会・学術大会, 2015年6月. 大谷知子, 賀勢泰子, 山本浩子, 國友一史, 川添和義, 佐藤陽一, 山内あい子 :
高齢者における腎機能評価に影響を与える因子の検討∼実測Ccr と推定Ccr の比較∼,
日本薬学会第135年会, 2015年3月. 川野壮一, 新谷保実, 鈴江朋子, 岡田直人, 佐藤陽一, 山内あい子 :
当院での糖尿病治療におけるDPP-4 阻害薬の有効性と安全性の評価,
日本薬学会第135年会, 2015年3月. 山本純, 森崎巧也, 北未来, 小宮千明, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
標的タンパク質の効率的濃縮および選択的ラベル化を可能とするチオール応答型トレーサブルリンカーの開発,
第32回メディシナルケミストリーシンポジウム, 2014年11月. 佐藤陽一, 常松晃希, 田嶋敦, 井本逸勢, 山内あい子, 岩本晃明 :
男性生殖能力関連候補遺伝子座 4SNPsの日本人男性を対象とした精液パラメータ及び男性不妊症との関連解析,
日本人類遺伝学会第59回大会, 2014年11月. 下澤辰也, 佐藤陽一, 岩本晃明, 山内あい子 :
日本人男性におけるY染色体上DAZ遺伝子の欠失パターンと精液パラメータとの関連解析,
日本人類遺伝学会第59回大会, 2014年11月. 関屋諒, 佐藤陽一, 山内あい子 :
Hemoglobinの抗酸化作用による腎機能保護に対するDPP4の影響,
第53回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2014年11月. 住斉, 田中和彦, 佐藤陽一, 針原伸二 :
9世紀の太政官令「飛騨の民の顔付は他国と違う」を裏打ちする状況;7世紀人骨と現代人DNAより,
第68回日本人類学会大会, 2014年11月. 住斉, 佐藤陽一, 針原伸二 :
母方先祖には先住民が，父方先祖には外来の権力民が現れる例;日本人平均と飛騨びと-DNA解析より,
第68回日本人類学会大会, 2014年11月. 中西智子, 石澤啓介, 阿部真治, 中瀬真理, 柴田洋文, 佐藤智恵美, 新垣尚捷, 佐藤陽一, 山﨑尚志, 笠原二郎, 東満美, 山﨑哲男, 山内あい子, 滝口祥令, 土屋浩一郎 :
アドバンスト演習を通した問題解決能力向上のための症例解析手法の検討-プロダクトからの分析,
日本薬学会第134年会, 2014年3月. 長尾麻以, 佐藤陽一, 山内あい子 :
炎症関連遺伝子多型とNSAIDs服用による発癌抑制に関するメタ解析,
日本薬学会第134年会, 2014年3月. 傳田将也, 山本純, 佐藤浩平, 坂本健, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
ラベル化試薬"SEAL-tag"の開発とCOX-1およびhCA1のラベル化,
日本薬学会第134年会, 2014年3月. 森崎巧也, 山本純, 重永章, 佐藤陽一, 猪熊翼, 山内あい子, 大髙章 :
標的タンパク質の効率的濃縮および選択的ラベル化を可能とする新規リンカー分子の開発,
日本薬学会第134年会, 2014年3月. 佐藤浩平, 傳田将也, 山本純, 坂本健, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
タンパク質機能解明を指向した新規ラベル化試薬''SEAL-tag''の開発研究,
第31回メディシナルケミストリーシンポジウム, 2013年11月. 佐藤陽一, 住斉, 針原伸二 :
飛騨では，ミトコンドリアDNAによる母系ルーツは土着縄文人，Y染色体による父方ルーツは渡来系弥生人,
第67回日本人類学会大会, 2013年11月. 住斉, 佐藤陽一, 針原伸二 :
飛騨びとのDNA解析で，母方ルーツは土着縄文人，父方ルーツは渡来系弥生人となっている超原-弥生男性における一夫多妻世代連鎖,
第67回日本人類学会大会, 2013年11月. 三上拓也, 佐藤陽一, 森山耕太, 宮髙紘輔, 岡田直人, 川添和義, 水口和生 :
高脂肪食ラットに及ぼすリンゴ酸の影響,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2013年10月. 中西智子, 石澤啓介, 阿部真治, 中瀬真理, 柴田洋文, 佐藤智恵美, 新垣尚捷, 佐藤陽一, 山﨑尚志, 笠原二郎, 東満美, 山﨑哲男, 山内あい子, 滝口祥令, 土屋浩一郎 :
アドバンスト演習を通した問題解決能力向上のための症例解析手法の検討,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2013年10月. 鎌田高宣, 佐藤陽一, 山内あい子 :
DPP4欠損ラット及び培養細胞を用いた腎臓におけるDPP4の機能解析,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2013年10月. 岩佐亮佑, 中谷充志, 佐藤陽一, 山内あい子 :
ヒト有機アニオントランスポーター3(hOAT3)新規アイソフォームのクローニングと発現解析,
第52回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2013年10月. 長尾麻以, 佐藤陽一, 山内あい子 :
NSAIDs服用による発癌リスク抑制効果とPPAR 遺伝子多型の影響に関するメタアナリシス,
第23回日本医療薬学会年会, 2013年9月. 山本純, 北未来, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
標的タンパク質精製・選択的ラベル化ツール''Traceable Linker''の開発研究,
生命分子機能研究会2013学術集会, 2013年9月. 長尾麻以, 佐藤陽一, 山内あい子 :
インターロイキン遺伝子多型とNSAIDs服用による発癌リスクに関するメタアナリシス,
第16回日本医薬品情報学会総会・学術大会, 2013年8月. 山本純, 北未来, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
標的タンパク質を釣り上げろ!∼トレーサブルリンカーの開発∼,
第45回若手ペプチド夏の勉強会, 2013年7月. 長尾麻以, 佐藤陽一, 山内あい子 :
NSAIDs服用による発癌抑制とIL遺伝子多型の影響に関するメタ解析,
日本薬学会第133回年会, 2013年3月. 鎌田高宣, 佐藤陽一, 山内あい子 :
腎機能障害発症におけるDPP4の関わり,
日本薬学会第133回年会, 2013年3月. 山本純, 北未来, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
標的タンパク質の精製・選択的ラベル化ツール「トレーサブルリンカー」の開発,
日本薬学会第133回年会, 2013年3月. 山本純, 傳田将也, 戎野紘司, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
フッ化物イオン応答型トレーサブルリンカーを利用したタンパク質精製実験,
第30回メディシナルケミストリーシンポジウム, 2012年11月. 中西智子, 佐藤陽一, 山内あい子, 土屋浩一郎 :
薬学部での臨床思考プロセスを取り入れた医薬品情報提供実習,
51回日本薬学会・日本薬剤師会，日本病院薬剤師会中国四国支部学術大会, 2012年11月. 傳田将也, 山本純, 坂本健, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
タンパク質選択的ラベル化法の開発研究,
第51回日本薬学会・日本薬剤師会,日本病院薬剤師会中国四国支部学術大会, 2012年11月. 鍋島亜季, 瀧澤みのり, 土居沙也子, 坂本久美子, 佐藤陽一, 山内あい子 :
ヒト胎盤絨毛細胞培養モデルによる薬物の胎盤通過性評価,
51回日本薬学会・日本薬剤師会，日本病院薬剤師会中国四国支部学術大会, 2012年11月. 鍛冶貴久, 佐藤陽一, 山内あい子 :
肥満発症とプロテアソーム活性の関連性,
51回日本薬学会・日本薬剤師会，日本病院薬剤師会中国四国支部学術大会, 2012年11月. 十塚正治, 佐藤陽一, 田中雅嗣 :
Y染色体，mtDNAの分析による縄文・弥生人分布,
第66回日本人類学会大会, 2012年11月. 長尾麻以, 佐藤陽一, 山内あい子 :
NSAIDs服用による発癌抑制とCOX遺伝子多型の影響に関するメタアナリシス,
第22回日本医療薬学会年会, 2012年10月. 岩出あゆみ, 吉田奈央, 坂本久美子, 佐藤陽一, 山内あい子 :
妊婦・授乳婦の漢方薬治療に関する安全性情報データベースの構築,
第22回日本医療薬学会年会, 2012年10月. 中島裕登, 稲山義高, 佐藤陽一, 山内あい子 :
薬物性腎障害に関する医薬品副作用情報の解析と機械学習による安全性予測,
第15回日本医薬品情報学会総会・学術大会, 2012年7月. 長尾麻以, 佐藤陽一, 山内あい子 :
COX-2遺伝子多型とNSAIDs服用による発がんリスクに関するメタアナリシス,
第15回日本医薬品情報学会総会・学術大会, 2012年7月. 庄野由佳理, 足立麻美, 坂本久美子, 佐藤陽一, 山内あい子 :
副作用情報に基づく薬物性肝障害惹起医薬品の分類と機械学習による安全性予測,
第15回日本医薬品情報学会総会・学術大会, 2012年7月. 傳田将也, 山本純, 佐藤浩平, 坂本健, 重永章, 佐藤陽一, 吉村好之, 山内あい子, 大髙章 :
新規タンパク質選択的ラベル化試薬''SEAL-tag''の開発研究,
日本ケミカルバイオロジー学会第7回年会, 2012年6月. 山本純, 傳田将也, 戎野紘司, 重永章, 佐藤陽一, 山内あい子, 大髙章 :
フッ化物イオン応答型トレーサブルリンカーを利用したタンパク質精製法の開発,
日本ケミカルバイオロジー学会第7回年会, 2012年6月. 吉田奈央, 村上貴子, 植村紘子, 小林理, 坂本久美子, 佐藤陽一, 山内あい子 :
妊婦と授乳婦の漢方薬治療における安全性情報データベースの構築,
第33 回徳島大学薬学部卒後教育公開講座, 2011年12月. 長尾麻以, 佐藤陽一, 山内あい子 :
NSAIDs 服用による発癌リスク抑制効果とCOX-2 遺伝子多型の影響に関するメタアナリシス,
第33 回徳島大学薬学部卒後教育公開講座, 2011年12月. 中西智子, 佐藤陽一, 山内あい子, 土屋浩一郎 :
問題解決能力の向上を目指した病院実務実習を考える,
第33 回徳島大学薬学部卒後教育公開講座, 2011年12月. 岡本茉佑子, 佐藤陽一, 三上拓也, 川添和義, 水口和生 :
五味子水抽出物の新規生理活性に関する研究,
第50回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2011年11月. 佐藤陽一, 新家利一, 中堀豊 :
Y染色体からみた日本人の成り立ち,
第65回日本人類学会大会, 2011年11月. 中西智子, 佐藤陽一, 山内あい子, 土屋浩一郎 :
問題解決能力の向上を目指した病院実務実習を考える,
第50回日本薬学会・日本薬剤師会・日本病院薬剤師会中国支部学術大会, 2011年11月. 佐藤陽一, 中西智子, 山内あい子 :
薬学部教員が病院実習「情報を正しく使う」を実施して,
第21回日本医療薬学会年会, 2011年10月. 山内あい子, 坂本久美子, 佐藤陽一 :
薬物の乳汁移行性予測について,
第14回日本医薬品情報学会, 2011年7月. 佐藤陽一, 中西智子, 山内あい子 :
初年度の病院実習「情報を正しく使う」を担当して,
第14回日本医薬品情報学会, 2011年7月. 吉田奈央, 佐藤陽一, 山内あい子 :
妊婦・授乳婦のための漢方安全性情報データベースの構築,
第14回日本医薬品情報学会総会・学術大会, 2011年7月. 佐藤陽一 :
五味子の新規作用,
第10回国際バイオEXPO, 2011年6月. 佐藤陽一, 中西智子, 山内あい子 :
初年度の病院実習「情報を正しく使う」を担当して,
日本薬学会131年会, 2011年3月. 佐藤陽一, 山内あい子 :
初年度の病院実習「情報を正しく使う」を担当して,
第31回徳島大学卒後教育公開講座, 2010年12月. 石澤啓介, 中西智子, 柴田洋文, 阿部真治, 杉村真由美, 奥村千恵子, 吉村好之, 佐藤陽一, 新垣尚捷, 川添和義, 東満美, 土屋浩一郎, 山内あい子, 山﨑哲男, 水口和生 :
徳島大学における薬学部・薬剤部連携による病院実務実習の実践,
第20回日本医療薬学会年会, 2010年11月. 太田佳那, 佐藤陽一, 岡本茉佑子, 阿部真治, エヴィス ABDEL AZIM アシュラフ, 勢井雅子, 川添和義, 水口和生 :
五味子水抽出物の新規作用に関する検討,
第49回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2010年11月. 庄野由佳理, 足立麻美, 坂本久美子, 佐藤陽一, 山内あい子 :
副作用情報に基づく薬物性肝障害惹起医薬品の分類と機械学習による安全性予測,
第38回構造活性相関シンポジウム, 2010年10月. 吉田恭史, Binh Quang Tran, 佐藤陽一, エヴィス ABDEL AZIM アシュラフ, 中堀豊, 井本逸勢 :
Y染色体多型における日本人男性とベトナム人男性の相違,
日本人類遺伝学会第55回大会, 2010年10月. 吉田友紀子, 岸江信介, 佐藤陽一, 中堀豊, 井本逸勢 :
集団遺伝学の立場から見た日本語音調成立についての考察,
日本人類遺伝学会第55回大会, 2010年10月. 佐藤陽一, 新家利一, エヴィス ABDEL AZIM アシュラフ, 中堀豊 :
性決定因子SRYの成り立ち,
日本遺伝学会第82回大会ワークショップ, 2010年9月. 佐藤陽一 :
Y染色体からみた日本人の成り立ち,
日本人類学会遺伝分科会公開シンポジウム, 2010年9月. 吉田友紀子, 前田和寿, 勢井雅子, 佐藤陽一, 中堀豊 :
先天性副腎皮質過形成に関する遺伝相談,
四国公衆衛生学会雑誌, Vol.55, No.1, 20-21, 2010年2月.

(キーワード): *遺伝相談

石澤啓介, 川添和義, 東満美, 土屋浩一郎, 山内あい子, 山﨑哲男, 水口和生, 中西智子, 柴田洋文, 阿部真治, 杉村真由美, 奥村千恵子, 吉村好之, 佐藤陽一, 新垣尚捷 :
P1-571 徳島大学における薬学部・薬剤部連携による病院実務実習の実践(一般演題ポスター発表,薬学教育(実務実習),臨床から学び臨床へと還元する医療薬学),
日本医療薬学会年会講演要旨集, Vol.20, No.0, 382, 2010年.

(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390845713041900800

(CiNii: 1390845713041900800) 腰岡桜, 佐藤陽一, 桐野靖, 紙本尚幸, 阿部真治, 川添和義, 中堀豊, 水口和生 :
DPP4欠損ラットを用いた1型糖尿病誘発モデルにおける脂質代謝異常発症のメカニズム解明,
第48回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 2009年11月. 佐藤陽一, 矢野尚二郎, 中堀豊 :
ヒト性決定因子SRYと相互作用する因子の同定と性分化に関わるメカニズムの解析,
第54回日本人類遺伝学会, 2009年9月. 坂本梢, 佐藤陽一, 勢井雅子, 中堀豊 :
肥満関連因子として見出されたProteasomeの脂肪分化に関わる機序の解析,
第54回日本人類遺伝学会, 2009年9月. 矢野尚二郎, 佐藤陽一, エヴィス ABDEL AZIM アシュラフ, 中堀豊 :
ヒト性決定因子であるSRYと相互作用するタンパク質の同定と解析,
第238回徳島医学会学術集会, 2009年2月. 勢井雅子, 石本寛子, 斎藤泰憲, 中川洋一, 中野卓郎, 佐藤陽一, 坂本梢, 吉田友紀子, 中堀豊 :
徳島県の糖尿病対策班の取り組み,
日本公衆衛生学会総会抄録集, 170, 2009年.

(キーワード): 糖尿病

紙本尚幸, 桐野靖, 腰岡桜, 佐藤陽一, 阿部真治, 川添和義, 水口和生, 中堀豊 :
ストレプトゾトシン誘発糖尿病ラットモデルにおけるDipptidyl pdptidase 4と脂質代謝異常の関連性について,
第47回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会, 83, 2008年11月. 佐藤陽一, 新家利一, 坂本梢, 矢野尚二郎, エヴィス ABDEL AZIM アシュラフ, 中堀豊 :
性決定因子SRYの発現調節に関わる因子の解析,
第53回日本人類遺伝学会, 2008年9月. 坂本梢, 佐藤陽一, 新家利一, 勢井雅子, 野村以徂子, 梅野真由美, エヴィス ABDEL AZIM アシュラフ, 中堀豊 :
プロテオミクス解析，遺伝子発現解析で見出された肥満関連因子の脂肪分化に関わる機序の解析,
第53回日本人類遺伝学会, 2008年9月. 矢野尚二郎, 佐藤陽一, エヴィス ABDEL AZIM アシュラフ, 中堀豊 :
ヒト性決定因子SRYと相互作用する因子の同定と解析,
第53回日本人類遺伝学会, 2008年9月. 紙本尚幸, 桐野靖, 佐藤陽一, 川添和義, 水口和生, 中堀豊 :
高カロリー食付加動物モデルを用いた，血中dipeptidyl peptidase 4(DPP4)活性と肥満ならびに耐糖能異常との関連性について,
日本薬学会年会, 2008年3月. 黒岩裕美, 佐藤陽一, 矢野尚二郎, 中堀豊 :
性決定因子であるSRYのGPR74発現に及ぼす影響,
第236回徳島医学会学術集会, 2008年2月. 佐藤陽一, 新家利一, 坂本梢, 黒岩裕美, 中堀豊 :
性決定遺伝子であるSRY遺伝子はDGCR8とSOX3のハイブリッドであり，TFCP2によって制御される,
第52回日本人類遺伝学会, 2007年9月. 坂本梢, 佐藤陽一, 新家利一, 勢井雅子, 野村以徂子, 中堀豊 :
肥満関連因子の探索と解析,
第52回日本人類遺伝学会, 2007年9月. 中野卓郎, 新家利一, 佐藤陽一, 勢井雅子, 木下桂午, 中堀豊, 梅野真由美 :
生活習慣病の発病リスクと遺伝的要因,
四国医学雑誌, Vol.62, 85, 2006年.

(キーワード): 危険因子

勢井雅子, 棟方百熊, 田中久子, 梅野真由美, 野村以徂子, 中野卓郎, 佐藤陽一, 吉田友紀子, 坂本梢, BinhTran Quang, 元木良江, 湯浅京子, 雄西智惠美, 新家利一, 中堀豊 :
地域・学校と連携した生活習慣病予防事業の実践,
四国公衆衛生学会雑誌, Vol.51, No.1, 29-30, 2006年.

(キーワード): *学校保健

閻洪涛, 新家利一, 陳剛, 木下桂午, 梅野真由美, 佐藤陽一, 中堀豊 :
ヒトSOX15の機能に関する研究,
第228回徳島医学会学術集会, 2003年2月.

研究会・報告書: 濱口惠寛, 佐藤陽一 :
Y染色体AZF領域の欠失からみた日本人男性,
第1回ヤポネシアゲノムくにうみミーティング, 2019年9月. 谷垣雄都, 佐藤陽一 :
Y染色体ハプログループによる表現型の特徴,
第1回ヤポネシアゲノムくにうみミーティング, 2019年9月. 佐藤陽一 :
ヤポネシアにおけるY染色体ハプログループの分布,
第1回ヤポネシアゲノムくにうみミーティング, 2019年9月. 藤井亜紀, 小薬鈴, 中川雄介, 田嶋敦, 井本逸勢, 山崎一恭, 岩本晃明, 佐藤陽一 :
ゲノムワイド関連解析によるInhibin B値関連遺伝子の同定と遺伝子改変マウスを用いたInhibin B発現に関する検討,
先端モデル動物支援プラットフォーム 2019年度若手支援技術講習会, 2019年9月. 野崎瑞貴, 上殿千晴, 古城公佑, 内田将央, 土屋春樹, 岩本晃明, 佐藤陽一 :
男性不妊症新規原因遺伝子の同定と遺伝子改変マウス作製による精子形成機能に関する検討,
先端モデル動物支援プラットフォーム平成30年度成果発表会, 2019年1月. 佐藤陽一 :
男性不妊症における新規原因遺伝子の同定に向けた2方向からのアプローチ,
2015年度ゲノム支援拡大班会議, 2015年8月. 佐藤陽一 :
男性不妊症における新規原因遺伝子の同定に向けた2方向からのアプローチ,
2014年度ゲノム支援拡大班会議, 2014年8月. 重永章, 山本純, 前田奈美, 佐藤陽一, 山内あい子, 大髙章 :
標的タンパク質の精製およびラベル化を可能とするトレーサブルリンカーの開発,
新学術領域研究「天然物ケミカルバイオロジー:分子標的と活性制御」第2回若手研究者ワークショップ, 2012年10月. 佐藤陽一, 新家利一, 中堀豊 :
ヒト性分化機構に対するプロテオミクスからのアプローチ,
特定領域研究「性分化機構の解明」第3回領域会議, 2006年9月. 佐藤陽一, 新家利一, 中堀豊 :
ヒト性分化機構に対するプロテオミクスからのアプローチ,
特定領域研究「性分化機構の解明」第2回領域会議, 2005年9月.

特許: Tomihiko Higuti, Hirofumi Shibata, Youichi Sato, Yoshihisa Takaishi, Kazuyoshi Kawazoe and Kotaro Murakami : Medical composition for treating infection with drugresistant Staphylococcus aureus, PIXXD2 WO 2004066992 A1 20040812 CAN 141:167738 AN 2004:648378 CAPLUS (Apr. 2004).
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: ALアミロイドーシスにおける単球に着目した疾患関連遺伝子と新規治療標的の探索 (研究課題/領域番号: 24K11516 )
男性不妊症新規原因遺伝子の同定と治療法の開発研究 (研究課題/領域番号: 22K09528 )
次世代シーケンサーを使用した精巣内精子採取術による精子回収予測システムの構築 (研究課題/領域番号: 19K22762 )
Y染色体からみたヤポネシア男性の起源・成立の解明 (研究課題/領域番号: 19H05346 )
検査および治療法の開発に向けた精子形成分子基盤の解明 (研究課題/領域番号: 17H04331 )
男性不妊症における新規原因遺伝子の同定に向けた２方向からのアプローチ (研究課題/領域番号: 26462461 )
データマイニング技術でヒトにおける医薬品の安全性を予測し得るか？ (研究課題/領域番号: 23590185 )
不妊症に関する遺伝子解析法および遺伝的背景の研究 (研究課題/領域番号: 20590576 )
早期糖尿病登録システムの構築および行動変容の要因分析 (研究課題/領域番号: 18390197 )
ヒト性分化機構に対するプロテオミクスからのアプローチ (研究課題/領域番号: 17052017 )
研究者番号（10363160）による検索

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2024年11月25日更新

専門分野・研究分野: 人類遺伝学 (Human Genetics)
所属学会・所属協会: 日本人類遺伝学会
日本薬学会
日本医薬品情報学会
日本分子生物学会
日本生殖医学会
日本生化学会
委員歴・役員歴: 日本人類遺伝学会 (評議員 [2004年5月])
日本薬学会 ( [2009年12月])
日本分子生物学会 ( [2015年7月])
日本生殖医学会 ( [2017年6月])
日本生化学会 ( [2018年6月])
受賞: 2004年8月, 第13回徳島医学会受賞 (徳島医学会)
活動: 研究者総覧に該当データはありませんでした。

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2024年11月24日更新

2024年11月23日更新

Ｊグローバル

Jグローバル最終確認日: 2024/11/23 01:23
氏名（漢字）: 佐藤陽一
氏名（フリガナ）: サトウヨウイチ
氏名（英字）: Sato Youichi
所属機関: 徳島大学准教授

リサーチマップ

researchmap最終確認日: 2024/11/24 01:35
氏名（漢字）: 佐藤陽一
氏名（フリガナ）: サトウヨウイチ
氏名（英字）: Sato Youichi
プロフィール: リサーチマップAPIで取得できませんでした。
登録日時: 2006/1/7 00:00
更新日時: 2024/11/19 09:32
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所属ID: 0344000000
所属: 徳島大学
部署: 大学院医歯薬学研究部医薬品情報学分野
職名: 准教授
学位: 薬学博士
学位授与機関: 徳島大学
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2024年11月23日更新

研究者番号: 10363160

所属（現在）: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学域), 教授

所属（過去の研究課題 情報に基づく）*注記: 2024/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学域), 教授
2022/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学域), 教授
2020/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学域), 教授
2018/4/1 – 2019/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学域), 准教授
2016/4/1 – 2017/4/1 : 徳島大学, 大学院医歯薬学研究部(薬学系), 准教授
2015/4/1 : 徳島大学, 大学院医歯薬学研究部, 准教授
2011/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 准教授
2010/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授
2007/4/1 – 2009/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教
2006/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助手
2005/4/1 – 2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助手

審査区分/研究分野

研究代表者

生物系 / 医歯薬学 / 外科系臨床医学 / 泌尿器科学
生物系
中区分58:社会医学、看護学およびその関連分野
複合領域
小区分56030:泌尿器科学関連

研究代表者以外

生物系 / 医歯薬学 / 社会医学 / 公衆衛生学・健康科学
生物系 / 医歯薬学 / 境界医学 / 病態検査学
生物系 / 医歯薬学 / 薬学 / 医療系薬学
小区分54010:血液および腫瘍内科学関連

キーワード

研究代表者

性分化 / SRY / プロテオミクス / GeneChip / 男性不妊症 / 精子濃度 / 次世代シーケンサー / GWAS / アンドロロジー / 男性不妊 / ゲノム / 遺伝子改変動物 / 次世代シーケンス / 遺伝子改変マウス / 非閉塞性無精子症 / micro-TESE / 機械学習 / MD-TESE / Y染色体 / ハプログループ

研究代表者以外

社会医学 / 糖尿病 / 早期介入 / 行動変容 / 栄養指導 / 不妊症 / 遺伝子解析 / 医薬品安全性情報 / データマイニング / ヒト毒性予測 / アミロイドーシス

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佐藤 陽一

Ｊグローバル

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佐藤陽一