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坂口末廣

徳島大学

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リサーチマップ・
Jグローバル
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2024年11月22日更新

職名: 教授
電話: 研究者総覧に該当データはありませんでした。
電子メール: sakaguch@ier.tokushima-u.ac.jp
学歴: 1994/3: 長崎大学大学院医学研究科博士課程修了
学位: 博士(医学) (長崎大学) (1994年3月)
職歴・経歴: 2006/4: 徳島大学教授, 分子酵素センター (-2007.3.)
2007/4: 徳島大学教授, 疾患酵素学研究センター (-2016.12.)
2017/1: 徳島大学教授, 先端酵素学研究所

専門分野・研究分野: 医学 (Medicine)

研究者総覧で最新データを確認する

2024年11月22日更新

専門分野・研究分野: 医学 (Medicine)
担当経験のある授業科目: プロテオミクス医科学特論 (大学院)
プロテオミクス概論 (大学院)
先端医学 (学部)
応用分子酵素学・病態学特論 (大学院)
技術英語入門 (学部)
生体情報統御学 (大学院)
生体情報統御学演習 (大学院)
生命科学の研究手法 (大学院)
研究室配属 (学部)
酵素・プロテオミクス医学実験実習・臨床研究実習 (大学院)
指導経験: 研究者総覧に該当データはありませんでした。

研究者総覧で最新データを確認する

2024年11月22日更新

専門分野・研究分野: 医学 (Medicine)

研究テーマ: プリオン病の病態解明 (プリオン, プリオン病, 神経変性, クロイツフェルト・ヤコブ病, 狂牛病)

著書

坂口末廣 :
シンプル微生物学(編集:小熊恵二，堀田博，若宮伸隆),
2018年3月. Keiji Uchiyama and Suehiro Sakaguchi :
Immunological strategies for the prevention and treatment of prion diseases.,
Caister Academic Press, Norfolk, UK, 2013.

(キーワード): プリオン

坂口末廣 :
44章スローウイルスとプリオン,
丸善出版, 東京, 2012年10月.

論文

Suehiro Sakaguchi and Hideyuki Hara :
The first non-prion pathogen identified: neurotropic influenza virus.,
Prion, Vol.16, No.1, 1-6, 2022.

(徳島大学機関リポジトリ): ● Metadata: 117592
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/19336896.2021.2015224
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34978525; ● Search Scopus @ Elsevier (PMID): 34978525; ● Search Scopus @ Elsevier (DOI): 10.1080/19336896.2021.2015224

(徳島大学機関リポジトリ: 117592, DOI: 10.1080/19336896.2021.2015224, PubMed: 34978525) Dini Agriani Pasiana, Hironori Miyata, Junji Chida, Hideyuki Hara, Morikazu Imamura, Ryuichiro Atarashi and Suehiro Sakaguchi :
Central residues in prion protein PrPC are crucial for its conversion into the pathogenic isoform,
The Journal of Biological Chemistry, Vol.298, No.9, 102381, 2022.

(キーワード): prion / Mice / Prion Diseases / Prion Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jbc.2022.102381
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35973512; ● Search Scopus @ Elsevier (PMID): 35973512; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jbc.2022.102381

(DOI: 10.1016/j.jbc.2022.102381, PubMed: 35973512) Hideyuki Hara and Suehiro Sakaguchi :
Virus Infection, Genetic Mutations, and Prion Infection in Prion Protein Conversion.,
International Journal of Molecular Sciences, Vol.22, No.22, 12439, 2021.

(徳島大学機関リポジトリ): ● Metadata: 117550
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms222212439
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34830321; ● Search Scopus @ Elsevier (PMID): 34830321; ● Search Scopus @ Elsevier (DOI): 10.3390/ijms222212439

(徳島大学機関リポジトリ: 117550, DOI: 10.3390/ijms222212439, PubMed: 34830321) Keiji Uchiyama, Hideyuki Hara, Junji Chida, Agriani Dini Pasiana, Morikazu Imamura, Tsuyoshi Mori, Hanae Takatsuki, Ryuichiro Atarashi and Suehiro Sakaguchi :
Ethanolamine Is a New Anti-Prion Compound,
International Journal of Molecular Sciences, Vol.22, No.21, 11742, 2021.

(要約): Prion diseases are a group of fatal neurodegenerative disorders caused by accumulation of proteinaceous infectious particles, or prions, which mainly consist of the abnormally folded, amyloidogenic prion protein, designated PrP. PrP is produced through conformational conversion of the cellular isoform of prion protein, PrP, in the brain. To date, no effective therapies for prion diseases have been developed. In this study, we incidentally noticed that mouse neuroblastoma N2a cells persistently infected with 22L scrapie prions, termed N2aC24L1-3 cells, reduced PrP levels when cultured in advanced Dulbecco's modified eagle medium (DMEM) but not in classic DMEM. PrP levels remained unchanged in prion-uninfected parent N2aC24 cells cultured in advanced DMEM. These results suggest that advanced DMEM may contain an anti-prion compound(s). We then successfully identified ethanolamine in advanced DMEM has an anti-prion activity. Ethanolamine reduced PrP levels in N2aC24L1-3 cells, but not PrP levels in N2aC24 cells. Also, oral administration of ethanolamine through drinking water delayed prion disease in mice intracerebrally inoculated with RML scrapie prions. These results suggest that ethanolamine could be a new anti-prion compound.
(キーワード): Animals / Brain / Cell Line, Tumor / Ethanolamine / Mice / Mice, Inbred ICR / PrPSc Proteins / Prion Diseases
(徳島大学機関リポジトリ): ● Metadata: 117551
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms222111742
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34769172; ● Summary page in Scopus @ Elsevier: 2-s2.0-85118175726

(徳島大学機関リポジトリ: 117551, DOI: 10.3390/ijms222111742, PubMed: 34769172, Elsevier: Scopus) Hideyuki Hara, Junji Chida, Keiji Uchiyama, Dini Agriani Pasiana, Etsuhisa Takahashi, Hiroshi Kido and Suehiro Sakaguchi :
Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells.,
Scientific Reports, Vol.11, No.1, 10109, 2021.

(徳島大学機関リポジトリ): ● Metadata: 117718
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-021-89586-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33980968; ● Search Scopus @ Elsevier (PMID): 33980968; ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-021-89586-6

(徳島大学機関リポジトリ: 117718, DOI: 10.1038/s41598-021-89586-6, PubMed: 33980968) Hideyuki Hara, Junji Chida, Agriani Dini Pasiana, Keiji Uchiyama, Yutaka Kikuchi, Tomoko Naito, Yuichi Takahashi, Junji Yamamura, Hisashi Kuromatsu and Suehiro Sakaguchi :
Vaporized Hydrogen Peroxide and Ozone Gas Synergistically Reduce Prion Infectivity on Stainless Steel Wire.,
International Journal of Molecular Sciences, Vol.22, No.6, 3268, 2021.

(要約): Prions are infectious agents causing prion diseases, which include Creutzfeldt-Jakob disease (CJD) in humans. Several cases have been reported to be transmitted through medical instruments that were used for preclinical CJD patients, raising public health concerns on iatrogenic transmissions of the disease. Since preclinical CJD patients are currently difficult to identify, medical instruments need to be adequately sterilized so as not to transmit the disease. In this study, we investigated the sterilizing activity of two oxidizing agents, ozone gas and vaporized hydrogen peroxide, against prions fixed on stainless steel wires using a mouse bioassay. Mice intracerebrally implanted with prion-contaminated stainless steel wires treated with ozone gas or vaporized hydrogen peroxide developed prion disease later than those implanted with control prion-contaminated stainless steel wires, indicating that ozone gas and vaporized hydrogen peroxide could reduce prion infectivity on wires. Incubation times were further elongated in mice implanted with prion-contaminated stainless steel wires treated with ozone gas-mixed vaporized hydrogen peroxide, indicating that ozone gas mixed with vaporized hydrogen peroxide reduces prions on these wires more potently than ozone gas or vaporized hydrogen peroxide. These results suggest that ozone gas mixed with vaporized hydrogen peroxide might be more useful for prion sterilization than ozone gas or vaporized hydrogen peroxide alone.
(キーワード): Animals / Brain / Disease Models, Animal / Dose-Response Relationship, Drug / Hydrogen Peroxide / Mice / Ozone / PrPC Proteins / Prion Diseases / Prions / Stainless Steel
(徳島大学機関リポジトリ): ● Metadata: 116339
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms22063268
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33806892; ● Summary page in Scopus @ Elsevier: 2-s2.0-85102845949

(徳島大学機関リポジトリ: 116339, DOI: 10.3390/ijms22063268, PubMed: 33806892, Elsevier: Scopus) Keiji Uchiyama, Miyata Hironori, Yamaguchi Yoshitaka, Imamura Morikazu, Okazaki Mariya, Pasiana Dini Agriani, Junji Chida, Hideyuki Hara, Atarashi Ryuichiro, Watanabe Hitomi, Kondoh Gen and Suehiro Sakaguchi :
Strain-Dependent Prion Infection in Mice Expressing Prion Protein with Deletion of Central Residues 91-106.,
International Journal of Molecular Sciences, Vol.21, No.19, 7260, 2020.

(要約): Conformational conversion of the cellular prion protein, PrP, into the abnormally folded isoform, PrP, is a key pathogenic event in prion diseases. However, the exact conversion mechanism remains largely unknown. Transgenic mice expressing PrP with a deletion of the central residues 91-106 were generated in the absence of endogenous PrP, designated Tg(PrP∆91-106)/ mice and intracerebrally inoculated with various prions. Tg(PrP∆91-106)/ mice were resistant to RML, 22L and FK-1 prions, neither producing PrP∆91-106 or prions in the brain nor developing disease after inoculation. However, they remained marginally susceptible to bovine spongiform encephalopathy (BSE) prions, developing disease after elongated incubation times and accumulating PrP∆91-106 and prions in the brain after inoculation with BSE prions. Recombinant PrP∆91-104 converted into PrP∆91-104 after incubation with BSE-PrP-prions but not with RML- and 22L-PrP-prions, in a protein misfolding cyclic amplification assay. However, digitonin and heparin stimulated the conversion of PrP∆91-104 into PrP∆91-104 even after incubation with RML- and 22L-PrP-prions. These results suggest that residues 91-106 or 91-104 of PrP are crucially involved in prion pathogenesis in a strain-dependent manner and may play a similar role to digitonin and heparin in the conversion of PrP into PrP.
(徳島大学機関リポジトリ): ● Metadata: 115634
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms21197260
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33019549; ● Summary page in Scopus @ Elsevier: 2-s2.0-85091919621

(徳島大学機関リポジトリ: 115634, DOI: 10.3390/ijms21197260, PubMed: 33019549, Elsevier: Scopus) Kishimoto Yasushi, Hirono Moritoshi, Atarashi Ryuichiro, Suehiro Sakaguchi, Yoshioka Tohru, Katamine Shigeru and Kirino Yutaka :
Impairment of cerebellar long-term depression and GABAergic transmission in prion protein deficient mice ectopically expressing PrPLP/Dpl.,
Scientific Reports, Vol.10, No.1, 15900, 2020.

(徳島大学機関リポジトリ): ● Metadata: 115618
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-020-72753-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32985542; ● Summary page in Scopus @ Elsevier: 2-s2.0-85091653225

(徳島大学機関リポジトリ: 115618, DOI: 10.1038/s41598-020-72753-6, PubMed: 32985542, Elsevier: Scopus) Hideyuki Hara and Suehiro Sakaguchi :
N-terminal Regions of Prion Protein: Functions and Roles in Prion Diseases.,
International Journal of Molecular Sciences, Vol.21, No.17, E6233, 2020.

(要約): The normal cellular isoform of prion protein, designated PrP, is constitutively converted to the abnormally folded, amyloidogenic isoform, PrP, in prion diseases, which include Creutzfeldt-Jakob disease in humans and scrapie and bovine spongiform encephalopathy in animals. PrP is a membrane glycoprotein consisting of the non-structural -terminal domain and the globular C-terminal domain. During conversion of PrP to PrP, its 2/3 C-terminal region undergoes marked structural changes, forming a protease-resistant structure. In contrast, the N-terminal region remains protease-sensitive in PrP. Reverse genetic studies using reconstituted PrP-knockout mice with various mutant PrP molecules have revealed that the N-terminal domain has an important role in the normal function of PrP and the conversion of PrP to PrP. The N-terminal domain includes various characteristic regions, such as the positively charged residue-rich polybasic region, the octapeptide repeat (OR) region consisting of five repeats of an octapeptide sequence, and the post-OR region with another positively charged residue-rich polybasic region followed by a stretch of hydrophobic residues. We discuss the normal functions of PrP, the conversion of PrP to PrP, and the neurotoxicity of PrP by focusing on the roles of the N-terminal regions in these topics.
(徳島大学機関リポジトリ): ● Metadata: 115633
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms21176233
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32872280; ● Summary page in Scopus @ Elsevier: 2-s2.0-85090181761

(徳島大学機関リポジトリ: 115633, DOI: 10.3390/ijms21176233, PubMed: 32872280, Elsevier: Scopus) Junji Chida, Hideyuki Hara, Keiji Uchiyama, Etsuhisa Takahashi, Hironori Miyata, Hidetaka Kosako, Yukiko Tomioka, Toshihiro Ito, Hiroyuki Horiuchi, Haruo Matsuda, Hiroshi Kido and Suehiro Sakaguchi :
Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice.,
PLoS Pathogens, Vol.16, No.8, e1008823, 2020.

(要約): The cellular prion protein, PrPC, is a glycosylphosphatidylinositol anchored-membrane glycoprotein expressed most abundantly in neuronal and to a lesser extent in non-neuronal cells. Its conformational conversion into the amyloidogenic isoform in neurons is a key pathogenic event in prion diseases, including Creutzfeldt-Jakob disease in humans and scrapie and bovine spongiform encephalopathy in animals. However, the normal functions of PrPC remain largely unknown, particularly in non-neuronal cells. Here we show that stimulation of PrPC with anti-PrP monoclonal antibodies (mAbs) protected mice from lethal infection with influenza A viruses (IAVs), with abundant accumulation of anti-inflammatory M2 macrophages with activated Src family kinases (SFKs) in infected lungs. A SFK inhibitor dasatinib inhibited M2 macrophage accumulation in IAV-infected lungs after treatment with anti-PrP mAbs and abolished the anti-PrP mAb-induced protective activity against lethal influenza infection in mice. We also show that stimulation of PrPC with anti-PrP mAbs induced M2 polarization in peritoneal macrophages through SFK activation in vitro and in vivo. These results indicate that PrPC could activate SFK in macrophages and induce macrophage polarization to an anti-inflammatory M2 phenotype after stimulation with anti-PrP mAbs, thereby eliciting protective activity against lethal infection with IAVs in mice after treatment with anti-PrP mAbs. These results also highlight PrPC as a novel therapeutic target for IAV infection.
(徳島大学機関リポジトリ): ● Metadata: 115613
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.ppat.1008823
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32845931; ● Search Scopus @ Elsevier (PMID): 32845931; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.ppat.1008823

(徳島大学機関リポジトリ: 115613, DOI: 10.1371/journal.ppat.1008823, PubMed: 32845931) Suehiro Sakaguchi and Junji Chida :
Prion Protein Is a Novel Modulator of Influenza: Its Potential Implications for Anti-Influenza Therapeutics.,
Current Issues in Molecular Biology, Vol.37, 21-32, 2019.

(徳島大学機関リポジトリ): ● Metadata: 115747
(出版サイトへのリンク): ● Publication site (DOI): 10.21775/cimb.037.021
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31814573; ● Summary page in Scopus @ Elsevier: 2-s2.0-85078510915

(徳島大学機関リポジトリ: 115747, DOI: 10.21775/cimb.037.021, PubMed: 31814573, Elsevier: Scopus) Nandita Rani Das, Hironori Miyata, Hideyuki Hara, Junji Chida, Keiji Uchiyama, Kentaro Masujin, Hitomi Watanabe, Gen Kondoh and Suehiro Sakaguchi :
The N-Terminal Polybasic Region of Prion Protein Is Crucial in Prion Pathogenesis Independently of the Octapeptide Repeat Region,
Molecular Neurobiology, Vol.57, 1203-1216, 2019.

(要約): Conformational conversion of the cellular isoform of prion protein, designated PrP, into the abnormally folded, amyloidogenic isoform, PrP, is an essential pathogenic event in prion diseases. However, the exact conversion mechanism remains largely unknown. Lines of evidence indicate that the N-terminal domain, which includes the N-terminal, positively charged polybasic region and the octapeptide repeat (OR) region, is important for PrP to convert into PrP after infection with prions. To further gain insights into the role of the polybasic region and the OR region in prion pathogenesis, we generated two different transgenic mice, designated Tg(PrP3K3A)/Prnp and Tg(PrP3K3A∆OR)/Prnp mice, which express PrP with lysine residues at codons 23, 24, and 27 in the polybasic region mutated with or without a deletion of the OR region on the Prnp background, respectively, and intracerebrally inoculated them with RML and 22L prions. We show that Tg(PrP3K3A)/Prnp mice were highly resistant to the prions, indicating that lysine residues at 23, 24, and 27 could be important for the polybasic region to support prion infection. Tg(PrP3K3A∆OR)/Prnp mice also had reduced susceptibility to RML and 22L prions equivalent to Tg(PrP3K3A)/Prnp mice. The pre-OR region, including the polybasic region, of PrP3K3A∆OR, but not PrP3K3A, was unusually converted to a protease-resistant structure during conversion to PrP3K3A∆OR. These results suggest that, while the OR region could affect the conformation of the polybasic region during conversion of PrP into PrP, the polybasic region could play a crucial role in prion pathogenesis independently of the OR region.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s12035-019-01804-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31707632; ● Summary page in Scopus @ Elsevier: 2-s2.0-85074844020

(DOI: 10.1007/s12035-019-01804-5, PubMed: 31707632, Elsevier: Scopus) Suehiro Sakaguchi and Junji Chida :
Roles of Prion Protein in Virus Infections.,
DNA and Cell Biology, Vol.37, No.10, 808-811, 2018.

(要約): The normal cellular prion protein, designated PrP, is a membrane glycoprotein expressed most abundantly in brains, particularly by neurons, and to a lesser extent in non-neuronal tissues including lungs. Conformational conversion of PrP into the amyloidogenic isoform is a key pathogenic event in prion diseases. We recently found that PrP has a protective role against infection with influenza A viruses (IAVs) in mice by reducing reactive oxygen species in the lungs after infection with IAVs. The antioxidative activity of PrP is probably attributable to its function to activate antioxidative enzyme Cu/Zn-superoxide dismutase, or SOD1, through regulating Cu content in lungs infected with IAVs. Oxidative stress could play a pivotal role in the pathogenesis of a wide range of viral infections. Here, we introduce our and others' studies on the role of PrP in viral infections, and raise the attractive possibility that PrP might be a novel target molecule for development of antioxidative therapeutics against not only IAV infection but also other viral infections.
(キーワード): Animals / Copper / Enzyme Activation / Epithelial Cells / Gene Expression Regulation / Heat-Shock Proteins / Influenza A virus / Lung / Mice / Orthomyxoviridae Infections / Oxidative Stress / PrPC Proteins / Protective Factors / Reactive Oxygen Species / Superoxide Dismutase-1
(徳島大学機関リポジトリ): ● Metadata: 112207
(出版サイトへのリンク): ● Publication site (DOI): 10.1089/dna.2018.4402
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30222366; ● Search Scopus @ Elsevier (PMID): 30222366; ● Search Scopus @ Elsevier (DOI): 10.1089/dna.2018.4402

(徳島大学機関リポジトリ: 112207, DOI: 10.1089/dna.2018.4402, PubMed: 30222366) Junji Chida, Hideyuki Hara, Masashi Yano, Keiji Uchiyama, Rani Nandita Das, Etsuhisa Takahashi, Hironori Miyata, Yukiko Tomioka, Toshihiro Ito, Hiroshi Kido and Suehiro Sakaguchi :
Prion protein protects mice from lethal infection with influenza A viruses.,
PLoS Pathogens, Vol.14, No.5, e1007049, 2018.

(要約): The cellular prion protein, designated PrPC, is a membrane glycoprotein expressed abundantly in brains and to a lesser extent in other tissues. Conformational conversion of PrPC into the amyloidogenic isoform is a key pathogenic event in prion diseases. However, the physiological functions of PrPC remain largely unknown, particularly in non-neuronal tissues. Here, we show that PrPC is expressed in lung epithelial cells, including alveolar type 1 and 2 cells and bronchiolar Clara cells. Compared with wild-type (WT) mice, PrPC-null mice (Prnp0/0) were highly susceptible to influenza A viruses (IAVs), with higher mortality. Infected Prnp0/0 lungs were severely injured, with higher inflammation and higher apoptosis of epithelial cells, and contained higher reactive oxygen species (ROS) than control WT lungs. Treatment with a ROS scavenger or an inhibitor of xanthine oxidase (XO), a major ROS-generating enzyme in IAV-infected lungs, rescued Prnp0/0 mice from the lethal infection with IAV. Moreover, Prnp0/0 mice transgenic for PrP with a deletion of the Cu-binding octapeptide repeat (OR) region, Tg(PrPOR)/Prnp0/0 mice, were also highly susceptible to IAV infection. These results indicate that PrPC has a protective role against lethal infection with IAVs through the Cu-binding OR region by reducing ROS in infected lungs. Cu content and the activity of anti-oxidant enzyme Cu/Zn-dependent superoxide dismutase, SOD1, were lower in Prnp0/0 and Tg(PrPOR)/Prnp0/0 lungs than in WT lungs. It is thus conceivable that PrPC functions to maintain Cu content and regulate SOD1 through the OR region in lungs, thereby reducing ROS in IAV-infected lungs and eventually protecting them from lethal infection with IAVs. Our current results highlight the role of PrPC in protection against IAV infection, and suggest that PrPC might be a novel target molecule for anti-influenza therapeutics.
(キーワード): Animals / Brain / Copper / Disease Susceptibility / Influenza A virus / Lung / Mice / Mice, Inbred C57BL / Mice, Knockout / Neurons / PrPC Proteins / Prion Diseases / Prion Proteins / Prions / Reactive Oxygen Species / Superoxide Dismutase
(徳島大学機関リポジトリ): ● Metadata: 112206
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.ppat.1007049
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29723291; ● Search Scopus @ Elsevier (PMID): 29723291; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.ppat.1007049

(徳島大学機関リポジトリ: 112206, DOI: 10.1371/journal.ppat.1007049, PubMed: 29723291) Luise Linsenmeier, Behnam Mohammadi, Sebastian Wetzel, Berta Puig, Walker S. Jackson, Alexander Hartmann, Keiji Uchiyama, Suehiro Sakaguchi, Kristina Endres, Jörg Tatzelt, Paul Saftig, Markus Glatzel and Hermann C. Altmeppen :
Structural and mechanistic aspects influencing the ADAM10-mediated shedding of the prion protein.,
Molecular Neurodegeneration, Vol.13, No.1, 18, 2018.

(要約): Proteolytic processing of the prion protein (PrP<superscript>C</superscript>) by endogenous proteases generates bioactive membrane-bound and soluble fragments which may help to explain the pleiotropic roles of this protein in the nervous system and in brain diseases. Shedding of almost full-length PrP<superscript>C</superscript> into the extracellular space by the metalloprotease ADAM10 is of peculiar relevance since soluble PrP stimulates axonal outgrowth and is protective in neurodegenerative conditions such as Alzheimer's and prion disease. However, molecular determinates and mechanisms regulating the shedding of PrP are entirely unknown. We produced an antibody recognizing the neo-epitope of shed PrP generated by ADAM10 in biological samples and used it to study structural and mechanistic aspects affecting the shedding. For this, we investigated genetically modified cellular and murine models by biochemical and morphological approaches. We show that the novel antibody specifically detects shed PrP in cell culture supernatants and murine brain. We demonstrate that ADAM10 is the exclusive sheddase of PrP<superscript>C</superscript> in the nervous system and reveal that the glycosylation state and type of membrane-anchorage of PrP<superscript>C</superscript> severely affect its shedding. Furthermore, we provide evidence that PrP shedding can be modulated by pharmacological inhibition and stimulation and present data suggesting that shedding is a relevant part of a compensatory network ensuring PrP<superscript>C</superscript> homeostasis of the cell. With the new antibody, our study introduces a new tool to reliably investigate PrP-shedding. In addition, this study provides novel and important insight into the regulation of this cleavage event, which is likely to be relevant for diagnostic and therapeutic approaches even beyond neurodegeneration.
(キーワード): ADAM10 Protein / Animals / 脳 (brain) / Mice / Neurons / Prion Proteins
(徳島大学機関リポジトリ): ● Metadata: 114545
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s13024-018-0248-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29625583; ● Summary page in Scopus @ Elsevier: 2-s2.0-85044987648

(徳島大学機関リポジトリ: 114545, DOI: 10.1186/s13024-018-0248-6, PubMed: 29625583, Elsevier: Scopus) Junji Chida and Suehiro Sakaguchi :
Cellular prion protein-mediated protection against influenza A virus infection.,
Future Virology, Vol.14, No.1, 31-37, 2018.

(徳島大学機関リポジトリ): ● Metadata: 113067
(出版サイトへのリンク): ● Publication site (DOI): 10.2217/fvl-2018-0146
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2217/fvl-2018-0146

(徳島大学機関リポジトリ: 113067, DOI: 10.2217/fvl-2018-0146) Hideyuki Hara, Miyata Hironori, Das Rani Nandita, Junji Chida, Yoshimochi Tatenobu, Keiji Uchiyama, Watanabe Hitomi, Kondoh Gen, Yokoyama Takashi and Suehiro Sakaguchi :
Prion Protein Devoid of the Octapeptide Repeat Region Delays BSE Pathogenesis in Mice.,
Journal of Virology, Vol.92, No.1, pii:e01368-17, 2018.

(要約): Conformational conversion of the cellular isoform of prion protein, PrP, into the abnormally folded, amyloidogenic isoform, PrP, is a key pathogenic event in prion diseases, including Creutzfeldt-Jakob disease in humans and scrapie and bovine spongiform encephalopathy (BSE) in animals. We previously reported that the octapeptide repeat (OR) region could be dispensable for converting PrP into PrP after infection with RML prions. We demonstrated that mice transgenically expressing mouse PrP with deletion of the OR region on the PrP knockout background, designated Tg(PrPΔOR)/ mice, did not show reduced susceptibility to RML scrapie prions, with abundant accumulation of PrPΔOR in their brains. We show here that Tg(PrPΔOR)/ mice were highly resistant to BSE prions, developing the disease with markedly elongated incubation times after infection with BSE prions. The conversion of PrPΔOR into PrPΔOR was markedly delayed in their brains. These results suggest that the OR region may have a crucial role in the conversion of PrP into PrP after infection with BSE prions. However, Tg(PrPΔOR)/ mice remained susceptible to RML and 22L scrapie prions, developing the disease without elongated incubation times after infection with RML and 22L prions. PrPΔOR accumulated only slightly less in the brains of RML- or 22L-infected Tg(PrPΔOR)/ mice than PrP in control wild-type mice. Taken together, these results indicate that the OR region of PrP could play a differential role in the pathogenesis of BSE prions and RML or 22L scrapie prions. Structure-function relationship studies of PrP conformational conversion into PrP are worthwhile to understand the mechanism of the conversion of PrP into PrP We show here that, by inoculating Tg(PrPΔOR)/ mice with the three different strains of RML, 22L, and BSE prions, the OR region could play a differential role in the conversion of PrP into PrP after infection with RML or 22L scrapie prions and BSE prions. PrPΔOR was efficiently converted into PrPΔOR after infection with RML and 22L prions. However, the conversion of PrPΔOR into PrPΔOR was markedly delayed after infection with BSE prions. Further investigation into the role of the OR region in the conversion of PrP into PrP after infection with BSE prions might be helpful for understanding the pathogenesis of BSE prions.
(キーワード): Animals / Brain / Cattle / Disease Susceptibility / Encephalopathy, Bovine Spongiform / Humans / Mice / Mice, Transgenic / Oligopeptides / PrPC Proteins / Prion Diseases / Prions / Sequence Deletion
(徳島大学機関リポジトリ): ● Metadata: 111465
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/JVI.01368-17
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29046443; ● CiNii @ 国立情報学研究所 (CRID): 1050845762396495104; ● Search Scopus @ Elsevier (PMID): 29046443; ● Search Scopus @ Elsevier (DOI): 10.1128/JVI.01368-17

(徳島大学機関リポジトリ: 111465, DOI: 10.1128/JVI.01368-17, PubMed: 29046443, CiNii: 1050845762396495104) Suehiro Sakaguchi and Keiji Uchiyama :
Novel Amplification Mechanism of Prions through Disrupting Sortilin-Mediated Trafficking.,
Prion, Vol.11, No.6, 398-404, 2017.

(要約): Conformational conversion of the cellular prion protein, PrP, into the abnormally folded isoform of prion protein, PrP, which leads to marked accumulation of PrP in brains, is a key pathogenic event in prion diseases, a group of fatal neurodegenerative disorders caused by prions. However, the exact mechanism of PrP accumulation in prion-infected neurons remains unknown. We recently reported a novel cellular mechanism to support PrP accumulation in prion-infected neurons, in which PrP itself promotes its accumulation by evading the cellular inhibitory mechanism, which is newly identified in our recent study. We showed that the VPS10P sorting receptor sortilin negatively regulates PrP accumulation in prion-infected neurons, by interacting with PrP and PrP and trafficking them to lysosomes for degradation. However, PrP stimulated lysosomal degradation of sortilin, disrupting the sortilin-mediated degradation of PrP and PrP and eventually evoking further accumulation of PrP in prion-infected neurons. These findings suggest a positive feedback amplification mechanism for PrP accumulation in prion-infected neurons.
(徳島大学機関リポジトリ): ● Metadata: 112200
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/19336896.2017.1391435
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29099278; ● Summary page in Scopus @ Elsevier: 2-s2.0-85034055921

(徳島大学機関リポジトリ: 112200, DOI: 10.1080/19336896.2017.1391435, PubMed: 29099278, Elsevier: Scopus) Keiji Uchiyama, Mitsuru Tomita, Masashi Yano, Junji Chida, Hideyuki Hara, Nandita Rani Das, Anders Nykjaer and Suehiro Sakaguchi :
Prions amplify through degradation of the VPS10P sorting receptor sortilin.,
PLoS Pathogens, Vol.13, No.6, e1006470, 2017.

(要約): Prion diseases are a group of fatal neurodegenerative disorders caused by prions, which consist mainly of the abnormally folded isoform of prion protein, PrPSc. A pivotal pathogenic event in prion disease is progressive accumulation of prions, or PrPSc, in brains through constitutive conformational conversion of the cellular prion protein, PrPC, into PrPSc. However, the cellular mechanism by which PrPSc is progressively accumulated in prion-infected neurons remains unknown. Here, we show that PrPSc is progressively accumulated in prion-infected cells through degradation of the VPS10P sorting receptor sortilin. We first show that sortilin interacts with PrPC and PrPSc and sorts them to lysosomes for degradation. Consistently, sortilin-knockdown increased PrPSc accumulation in prion-infected cells. In contrast, overexpression of sortilin reduced PrPSc accumulation in prion-infected cells. These results indicate that sortilin negatively regulates PrPSc accumulation in prion-infected cells. The negative role of sortilin in PrPSc accumulation was further confirmed in sortilin-knockout mice infected with prions. The infected mice had accelerated prion disease with early accumulation of PrPSc in their brains. Interestingly, sortilin was reduced in prion-infected cells and mouse brains. Treatment of prion-infected cells with lysosomal inhibitors, but not proteasomal inhibitors, increased the levels of sortilin. Moreover, sortilin was reduced following PrPSc becoming detectable in cells after infection with prions. These results indicate that PrPSc accumulation stimulates sortilin degradation in lysosomes. Taken together, these results show that PrPSc accumulation of itself could impair the sortilin-mediated sorting of PrPC and PrPSc to lysosomes for degradation by stimulating lysosomal degradation of sortilin, eventually leading to progressive accumulation of PrPSc in prion-infected cells.
(キーワード): prion / Sortilin / VPS10P
(徳島大学機関リポジトリ): ● Metadata: 110433
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.ppat.1006470
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28665987; ● Search Scopus @ Elsevier (PMID): 28665987; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.ppat.1006470

(徳島大学機関リポジトリ: 110433, DOI: 10.1371/journal.ppat.1006470, PubMed: 28665987) Das Rani Nandita, Miyata Hironori, Hideyuki Hara, Keiji Uchiyama, Junji Chida, Masashi Yano, Watanabe Hitomi, Kondoh Gen and Suehiro Sakaguchi :
Effects of prion protein devoid of the N-terminal residues 25-50 on prion pathogenesis in mice.,
Archives of Virology, Vol.162, No.7, 1867-1876, 2017.

(要約): The N-terminal polybasic region of the normal prion protein, PrP(C), which encompasses residues 23-31, is important for prion pathogenesis by affecting conversion of PrP(C) into the pathogenic isoform, PrP(Sc). We previously reported transgenic mice expressing PrP with residues 25-50 deleted in the PrP-null background, designated as Tg(PrPpreOR)/Prnp (0/0) mice. Here, we produced two new lines of Tg(PrPpreOR)/Prnp (0/0) mice, each expressing the mutant protein, PrPpreOR, 1.1 and 1.6 times more than PrP(C) in wild-type mice, and subsequently intracerebrally inoculated RML and 22L prions into them. The lower expresser showed slightly reduced susceptibility to RML prions but not to 22L prions. The higher expresser exhibited enhanced susceptibility to both prions. No prion transmission barrier was created in Tg(PrPpreOR)/Prnp (0/0) mice against full-length PrP(Sc). PrP(Sc)preOR accumulated in the brains of infected Tg(PrPpreOR)/Prnp (0/0) mice less than PrP(Sc) in control wild-type mice, although lower in RML-infected Tg(PrPpreOR)/Prnp (0/0) mice than in 22L-infected mice. Prion infectivity in infected Tg(PrPpreOR)/Prnp (0/0) mice was also lower than that in wild-type mice. These results indicate that deletion of residues 25-50 only slightly affects prion susceptibility, the conversion of PrP(C) into PrP(Sc), and prion infectivity in a strain-specific way. PrPpreOR retains residues 23-24 and lacks residues 25-31 in the polybasic region. It is thus conceivable that residues 23-24 rather than 25-31 are important for the polybasic region to support prion pathogenesis. However, other investigators have reported that residues 27-31 not 23-24 are important to support prion pathogenesis. Taken together, the polybasic region might support prion pathogenesis through multiple sites including residues 23-24 and 27-31.
(キーワード): Amino Acid Sequence / Animals / Disease Susceptibility / Mice / Mice, Inbred C57BL / Mice, Transgenic / Prion Diseases / Prion Proteins / Repetitive Sequences, Amino Acid / Sequence Deletion
(徳島大学機関リポジトリ): ● Metadata: 110119
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00705-017-3295-3
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28255815; ● Summary page in Scopus @ Elsevier: 2-s2.0-85014121673

(徳島大学機関リポジトリ: 110119, DOI: 10.1007/s00705-017-3295-3, PubMed: 28255815, Elsevier: Scopus) Taichi Hamanaka, Keiko Nishizawa, Yuji Sakasegawa, Ayumi Oguma, Kenta Teruya, Hiroshi Kurahashi, Hideyuki Hara, Suehiro Sakaguchi and Katsumi Doh-ura :
Melanin or melanin-like substance interacts with the N-terminal portion of prion protein and inhibits abnormal prion protein formation in prion-infected cells.,
Journal of Virology, Vol.91, No.6, e01862-16, 2017.

(要約): Prion diseases are progressive fatal neurodegenerative illnesses caused by the accumulation of transmissible abnormal prion protein (PrP). To find treatments for prion diseases, we searched for substances from natural resources that inhibit abnormal PrP formation in prion-infected cells. We found that high-molecular-weight components from insect cuticle extracts reduced abnormal PrP levels. The chemical nature of these components was consistent with that of melanin. In fact, synthetic melanin produced from tyrosine or 3-hydroxy-l-tyrosine inhibited abnormal PrP formation. Melanin did not modify cellular or cell surface PrP levels, nor did it modify lipid raft or cellular cholesterol levels. Neither did it enhance autophagy or lysosomal function. Melanin was capable of interacting with PrP at two N-terminal domains. Specifically, it strongly interacted with the PrP region of amino acids 23 to 50 including a positively charged amino acid cluster and weakly interacted with the PrP octarepeat peptide region of residues 51 to 90. However, the in vitro and in vivo data were inconsistent with those of prion-infected cells. Abnormal PrP formation in protein misfolding cyclic amplification was not inhibited by melanin. Survival after prion infection was not significantly altered in albino mice or exogenously melanin-injected mice compared with that of control mice. These data suggest that melanin, a main determinant of skin color, is not likely to modify prion disease pathogenesis, even though racial differences in the incidence of human prion diseases have been reported. Thus, the findings identify an interaction between melanin and the N terminus of PrP, but the pathophysiological roles of the PrP-melanin interaction remain unclear.IMPORTANCE The N-terminal region of PrP is reportedly important for neuroprotection, neurotoxicity, and abnormal PrP formation, as this region is bound by many factors, such as metal ions, lipids, nucleic acids, antiprion compounds, and several proteins, including abnormal PrP in prion disease and the Aβ oligomer in Alzheimer's disease. In the present study, melanin, a main determinant of skin color, was newly found to interact with this N-terminal region and inhibits abnormal PrP formation in prion-infected cells. However, the data for prion infection in mice lacking melanin production suggest that melanin is not associated with the prion disease mechanism, although the incidence of prion disease is reportedly much higher in white people than in black people. Thus, the roles of the PrP-melanin interaction remain to be further elucidated, but melanin might be a useful competitive tool for evaluating the functions of other ligands at the N-terminal region.
(キーワード): Animals / Cell Line / Melanins / Mice / Neurons / Prion Diseases / Prions / Protein Binding / Protein Interaction Mapping / Survival Analysis
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/JVI.01862-16
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28077650; ● Search Scopus @ Elsevier (PMID): 28077650; ● Search Scopus @ Elsevier (DOI): 10.1128/JVI.01862-16

(DOI: 10.1128/JVI.01862-16, PubMed: 28077650) Yuya Nagasawa, Yu Takahashi, Wataru Itani, Hitoshi Watanabe, Yusuke Hidaka, Shotaro Morita, Kei Suzuki, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Morikazu Imamura, Takashi Yokoyama, Motohiro Horiuchi, Suehiro Sakaguchi, Shirou Mohri, Michael T. Rose, Tomonori Nochi and Hisashi Aso :
Prion Protein Binds to Aldolase A Produced by Bovine Intestinal M Cells.,
Open Journal of Veterinary Medicine, Vol.5, No.3, 43-60, 2015.

(出版サイトへのリンク): ● Publication site (DOI): 10.4236/ojvm.2015.53007
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.4236/ojvm.2015.53007

(DOI: 10.4236/ojvm.2015.53007) Keiji Uchiyama, Miyata Hironori, Masashi Yano, Yoshitaka Yamaguti, Imamura Morikazu, Muramatsu Naomi, Das Rani Nandita, Junji Chida, Hideyuki Hara and Suehiro Sakaguchi :
Mouse-Hamster Chimeric Prion Protein (PrP) Devoid of N-terminal Residues 23-88 Restores Susceptibility to 22L Prions, But Not to RML Prions in PrP-Knockout Mice.,
PLoS ONE, Vol.9, No.10, e109737, 2014.

(要約): Prion infection induces conformational conversion of the normal prion protein PrPC, into the pathogenic isoform PrPSc, in prion diseases. It has been shown that PrP-knockout (Prnp0/0) mice transgenically reconstituted with a mouse-hamster chimeric PrP lacking N-terminal residues 23-88, or Tg(MHM223-88)/Prnp 0/0 mice, neither developed the disease nor accumulated MHM2Sc23-88 in their brains after inoculation with RML prions. In contrast, RML-inoculated Tg(MHM223-88)/Prnp 0/+ mice developed the disease with abundant accumulation of MHM2Sc23-88 in their brains. These results indicate that MHM223-88 itself might either lose or greatly reduce the converting capacity to MHM2Sc23-88, and that the co-expressing wild-type PrPC can stimulate the conversion of MHM223-88 to MHM2Sc23-88 in trans. In the present study, we confirmed that Tg(MHM223-88)/Prnp 0/0 mice remained resistant to RML prions for up to 730 days after inoculation. However, we found that Tg(MHM223-88)/Prnp 0/0 mice were susceptible to 22L prions, developing the disease with prolonged incubation times and accumulating MHM2Sc23-88 in their brains. We also found accelerated conversion of MHM223-88 into MHM2Sc23-88 in the brains of RML- and 22L-inoculated Tg(MHM223-88)/Prnp 0/+ mice. However, wild-type PrPSc accumulated less in the brains of these inoculated Tg(MHM223-88)/Prnp 0/+ mice, compared with RML- and 22L-inoculated Prnp 0/+ mice. These results show that MHM223-88 itself can convert into MHM2Sc23-88 without the help of the trans-acting PrPC, and that, irrespective of prion strains inoculated, the co-expressing wild-type PrPC stimulates the conversion of MHM223-88 into MHM2Sc23-88, but to the contrary, the co-expressing MHM223-88 disturbs the conversion of wild-type PrPC into PrPSc.
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0109737
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25330286; ● Search Scopus @ Elsevier (PMID): 25330286; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0109737

(DOI: 10.1371/journal.pone.0109737, PubMed: 25330286) Keiji Uchiyama, Miyata Hironori and Suehiro Sakaguchi :
Disturbed vesicular trafficking of membrane proteins in prion disease.,
Prion, Vol.7, No.6, 447-451, 2013.

(要約): The pathogenic mechanism of prion diseases remains unknown. We recently reported that prion infection disturbs post-Golgi trafficking of certain types of membrane proteins to the cell surface, resulting in reduced surface expression of membrane proteins and abrogating the signal from the proteins. The surface expression of the membrane proteins was reduced in the brains of mice inoculated with prions, well before abnormal symptoms became evident. Prions or pathogenic prion proteins were mainly detected in endosomal compartments, being particularly abundant in recycling endosomes. Some newly synthesized membrane proteins are delivered to the surface from the Golgi apparatus through recycling endosomes, and some endocytosed membrane proteins are delivered back to the surface through recycling endosomes. These results suggest that prions might cause neuronal dysfunctions and cell loss by disturbing post-Golgi trafficking of membrane proteins via accumulation in recycling endosomes. Interestingly, it was recently shown that delivery of a calcium channel protein to the cell surface was impaired and its function was abrogated in a mouse model of hereditary prion disease. Taken together, these results suggest that impaired delivery of membrane proteins to the cell surface is a common pathogenic event in acquired and hereditary prion diseases.
(出版サイトへのリンク): ● Publication site (DOI): 10.4161/pri.27381
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24335150; ● Summary page in Scopus @ Elsevier: 2-s2.0-84893871953

(DOI: 10.4161/pri.27381, PubMed: 24335150, Elsevier: Scopus) Kishimoto Yaushi, Hirono Moritoshi, Atarashi Ryuichiro, Suehiro Sakaguchi, Yoshioka Tohru, Katamine Shigeru and Kirino Yutaka :
Age-dependent impairment of eyeblink conditioning in prion protein-deficient mice.,
PLoS ONE, Vol.8, No.4, e60627, 2013.

(要約): Mice lacking the prion protein (PrPC) gene (Prnp), Ngsk Prnp0/0 mice, show late-onset cerebellar Purkinje cell (PC) degeneration because of ectopic overexpression of PrPC-like protein (PrPLP/Dpl). Because PrPC is highly expressed in cerebellar neurons (including PCs and granule cells), it may be involved in cerebellar synaptic function and cerebellar cognitive function. However, no studies have been conducted to investigate the possible involvement of PrPC and/or PrPLP/Dpl in cerebellum-dependent discrete motor learning. Therefore, the present cross-sectional study was designed to examine cerebellum-dependent delay eyeblink conditioning in Ngsk Prnp0/0 mice in adulthood (16, 40, and 60 weeks of age). The aims of the present study were two-fold: (1) to examine the role of PrPC and/or PrPLP/Dpl in cerebellum-dependent motor learning and (2) to confirm the age-related deterioration of eyeblink conditioning in Ngsk Prnp0/0 mice as an animal model of progressive cerebellar degeneration. Ngsk Prnp0/0 mice aged 16 weeks exhibited intact acquisition of conditioned eyeblink responses (CRs), although the CR timing was altered. The same result was observed in another line of PrPc-deficient mice, ZrchI PrnP0/0 mice. However, at 40 weeks of age, CR incidence impairment was observed in Ngsk Prnp0/0 mice. Furthermore, Ngsk Prnp0/0 mice aged 60 weeks showed more significantly impaired CR acquisition than Ngsk Prnp0/0 mice aged 40 weeks, indicating the temporal correlation between cerebellar PC degeneration and motor learning deficits. Our findings indicate the importance of the cerebellar cortex in delay eyeblink conditioning and suggest an important physiological role of prion protein in cerebellar motor learning.
(キーワード): Aging / Animals / Base Sequence / Blinking / Conditioning, Operant / DNA Primers / Mice / Polymerase Chain Reaction / Prions
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0060627
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23593266; ● CiNii @ 国立情報学研究所 (CRID): 1050850247205350016; ● Search Scopus @ Elsevier (PMID): 23593266; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0060627

(DOI: 10.1371/journal.pone.0060627, PubMed: 23593266, CiNii: 1050850247205350016) Kimura Tsutomu, Sako Takeo, (名) Siqin, Hosokawa-Muto Junji, Cui Long Yi, Wada Yasuhiro, Kataoka Yosky, Doi Hisashi, Suehiro Sakaguchi, Suzuki Masaaki, Watanabe Yasuyoshi and Kuwata Kazuo :
Synthesis of an (11) C-labeled antiprion GN8 derivative and evaluation of its brain uptake by positron emission tomography.,
ChemMedChem, Vol.8, No.7, 1035-1039, 2013.

(要約): A radiolabeled PET! A (11) C-labeled derivative of N,N'-(methylenedi-4,1-phenylene)bis[2-(1-pyrrolidinyl) acetamide] (GN8), an antiprion agent currently under development, was synthesized by palladium-catalyzed rapid methylation of aryltributylstannane and assessed for brain penetration and organ distribution in rats by positron emission tomography (PET).
(キーワード): Acetamides / Animals / Brain / Carbon Radioisotopes / Humans / Molecular Structure / Positron-Emission Tomography / Prions / Tissue Distribution
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/cmdc.201300167
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23712919; ● Summary page in Scopus @ Elsevier: 2-s2.0-84879844540

(DOI: 10.1002/cmdc.201300167, PubMed: 23712919, Elsevier: Scopus) Keiji Uchiyama, Naomi Muramatsu, Masashi Yano, Takeshi Usui, Hironori Miyata and Suehiro Sakaguchi :
Prions disturb post-Golgi trafficking of membrane proteins.,
Nature Communications, Vol.4, 1846, 2013.

(要約): Conformational conversion of normal cellular prion protein PrP(C) into pathogenic PrP(Sc) is central to the pathogenesis of prion diseases. However, the pathogenic mechanism remains unknown. Here we show that post-Golgi vesicular trafficking is significantly delayed in prion-infected N2a cells. Accordingly, cell surface expression of membrane proteins examined, including PrP(C), insulin receptor involved in neuroprotection, and attractin, whose mutation causes prion disease-like spongiform neurodegeneration, is reduced. Instead, they accumulate in the Golgi apparatus. PrP(Sc) is detected throughout endosomal compartments, being particularly abundant in recycling endosome. We also show reduced surface expression of PrP(C) and insulin receptor in prion-infected mouse brains well before the onset of disease. These results suggest that prion infection might impair post-Golgi trafficking of membrane proteins to the cell surface in neurons via PrP(Sc) accumulated in recycling endosome, and eventually induce neuronal dysfunctions associated with prion diseases.
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/ncomms2873
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23673631; ● Search Scopus @ Elsevier (PMID): 23673631; ● Search Scopus @ Elsevier (DOI): 10.1038/ncomms2873

(DOI: 10.1038/ncomms2873, PubMed: 23673631) Yoshitaka Yamaguti, Hironori Miyata, Keiji Uchiyama, Akira Ootsuyama, Sachiko Inubushi, Tsuyoshi Mori, Naomi Muramatsu, Shigeru Katamine and Suehiro Sakaguchi :
Biological and biochemical characterization of mice expressing prion protein devoid of the octapeptide repeat region after infection with prions.,
PLoS ONE, Vol.7, No.8, e43540, 2012.

(要約): Accumulating lines of evidence indicate that the N-terminal domain of prion protein (PrP) is involved in prion susceptibility in mice. In this study, to investigate the role of the octapeptide repeat (OR) region alone in the N-terminal domain for the susceptibility and pathogenesis of prion disease, we intracerebrally inoculated RML scrapie prions into tg(PrPOR)/Prnp(0/0) mice, which express mouse PrP missing only the OR region on the PrP-null background. Incubation times of these mice were not extended. Protease-resistant PrPOR, or PrP(Sc)OR, was easily detectable but lower in the brains of these mice, compared to that in control wild-type mice. Consistently, prion titers were slightly lower and astrogliosis was milder in their brains. However, in their spinal cords, PrP(Sc)OR and prion titers were abundant and astrogliosis was as strong as in control wild-type mice. These results indicate that the role of the OR region in prion susceptibility and pathogenesis of the disease is limited. We also found that the PrP(Sc)OR, including the pre-OR residues 23-50, was unusually protease-resistant, indicating that deletion of the OR region could cause structural changes to the pre-OR region upon prion infection, leading to formation of a protease-resistant structure for the pre-OR region.
(キーワード): Amino Acid Sequence / Animals / Disease Susceptibility / Endopeptidase K / Forelimb / Gene Expression Regulation / Lysine / Mice / Mice, Inbred C57BL / Mice, Transgenic / Oligopeptides / Paresis / Prions / Repetitive Sequences, Amino Acid / Scrapie / Sequence Deletion
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0043540
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22927985; ● Search Scopus @ Elsevier (PMID): 22927985; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0043540

(DOI: 10.1371/journal.pone.0043540, PubMed: 22927985) Toshiya Kubota, Yuta Hamazoe, Shuhei Hashiguchi, Daisuke Ishibashi, Kazuyuki Akasaka, Noriyuki Nishida, Shigeru Katamine, Suehiro Sakaguchi, Ryota Kuroki, Toshihiro Nakashima and Kazuhisa Sugimura :
Direct evidence of generation and accumulation of -sheet-rich prion protein in scrapie-infected neuroblastoma cells with human IgG1 antibody specific for -form prion protein.,
The Journal of Biological Chemistry, Vol.287, No.17, 14023-14039, 2012.

(要約): We prepared -sheet-rich recombinant full-length prion protein (-form PrP) (Jackson, G. S., Hosszu, L. L., Power, A., Hill, A. F., Kenney, J., Saibil, H., Craven, C. J., Waltho, J. P., Clarke, A. R., and Collinge, J. (1999) Science 283, 1935-1937). Using this -form PrP and a human single chain Fv-displaying phage library, we have established a human IgG1 antibody specific to -form but not -form PrP, PRB7 IgG. When prion-infected ScN2a cells were cultured with PRB7 IgG, they generated and accumulated PRB7-binding granules in the cytoplasm with time, consequently becoming apoptotic cells bearing very large PRB7-bound aggregates. The SAF32 antibody recognizing the N-terminal octarepeat region of full-length PrP stained distinct granules in these cells as determined by confocal laser microscopy observation. When the accumulation of proteinase K-resistant PrP was examined in prion-infected ScN2a cells cultured in the presence of PRB7 IgG or SAF32, it was strongly inhibited by SAF32 but not at all by PRB7 IgG. Thus, we demonstrated direct evidence of the generation and accumulation of -sheet-rich PrP in ScN2a cells de novo. These results suggest first that PRB7-bound PrP is not responsible for the accumulation of -form PrP aggregates, which are rather an end product resulting in the triggering of apoptotic cell death, and second that SAF32-bound PrP lacking the PRB7-recognizing -form may represent so-called PrP(Sc) with prion propagation activity. PRB7 is the first human antibody specific to -form PrP and has become a powerful tool for the characterization of the biochemical nature of prion and its pathology.
(キーワード): Animals / Antibodies / Circular Dichroism / Genetic Engineering / Humans / Immunoglobulin G / Immunohistochemistry / Mice / Microscopy, Atomic Force / Microscopy, Confocal / Neuroblastoma / Peptide Library / Prions / Protein Conformation / Protein Folding / Protein Structure, Secondary / Scrapie
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M111.318352
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22356913; ● Search Scopus @ Elsevier (PMID): 22356913; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M111.318352

(DOI: 10.1074/jbc.M111.318352, PubMed: 22356913) Ishibashi Daisuke, Yamanaka Hitoki, Tsuyoshi Mori, Yamaguchi Naohiro, Yoshitaka Yamaguti, Nishida Noriyuki and Suehiro Sakaguchi :
Antigenic mimicry-mediated anti-prion effects induced by bacterial enzyme succinylarginine dihydrolase in mice.,
Vaccine, Vol.29, No.50, 9321-9328, 2011.

(要約): Prions, the causative agents of prion diseases, are immunologically tolerated because their major component, prion protein (PrP), is a host-encoded molecule. Therefore, no effective prion vaccines have been developed. We previously showed that heterologous bovine and sheep PrP immunizations of mice overcame tolerance by an antigenic mimicry mechanism to efficiently induce anti-PrP auto-antibodies (Abs), significantly prolonging incubation times in mice subsequently infected with the mouse-adapted Fukuoka-1 prion. These results prompted us to investigate if non-mammal derived molecules able to antigenically mimic anti-prion epitopes, could act as prion vaccines. We show here that immunization of mice with recombinant succinylarginine dihydrolase, a bacterial enzyme with a peptide sequence similar to an anti-prion epitope, induced anti-PrP auto-Abs with anti-prion activity and significantly retarded survival times of the mice subsequently infected with Fukuoka-1 prions. These results might open a way for development of a new type of antigenic mimicry-based prion vaccine.
(キーワード): Animals / Antibody Formation / Autoantibodies / Bacterial Proteins / Epitopes / Female / Hydrolases / Immunization / Mice / Mice, Inbred BALB C / Molecular Mimicry / Prion Diseases / Prions / Recombinant Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.vaccine.2011.10.017
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22008817; ● Search Scopus @ Elsevier (PMID): 22008817; ● Search Scopus @ Elsevier (DOI): 10.1016/j.vaccine.2011.10.017

(DOI: 10.1016/j.vaccine.2011.10.017, PubMed: 22008817) Ikuro Takakura, Kohtaro Miyazawa, Takashi Kanaya, Wataru Itani, Kouichi Watanabe, Shyuichi Ohwada, Hitoshi Watanabe, Tetsuya Hondo, T Michael Rose, Tsuyoshi Mori, Suehiro Sakaguchi, Noriyuki Nishida, Shigeru Katamine, Takahiro Yamaguchi and Hisashi Aso :
Orally administered prion protein is incorporated by m cells and spreads into lymphoid tissues with macrophages in prion protein knockout mice.,
The American Journal of Pathology, Vol.179, No.3, 1301-1309, 2011.

(要約): Transmissible spongiform encephalopathies are fatal neurodegenerative diseases. Infection by the oral route is assumed to be important, although its pathogenesis is not understood. Using prion protein (PrP) knockout mice, we investigated the sequence of events during the invasion of orally administered PrPs through the intestinal mucosa and the spread into lymphoid tissues and the peripheral nervous system. Orally administered PrPs were incorporated by intestinal epitheliocytes in the follicle-associated epithelium and villi within 1 hour. PrP-positive cells accumulated in the subfollicle region of Peyer's patches a few hours thereafter. PrP-positive cells spread toward the mesenteric lymph nodes and spleen after the accumulation of PrPs in the Peyer's patches. The number of PrP molecules in the mesenteric lymph nodes and spleen peaked at 2 days and 6 days after inoculation, respectively. The epitheliocytes in the follicle-associated epithelium incorporating PrPs were annexin V-positive microfold cells and PrP-positive cells in Peyer's patches and spleen were CD11b-positive and CD14-positive macrophages. Additionally, PrP-positive cells in Peyer's patches and spleen were detected in the vicinity of peripheral nerve fibers in the early stages of infection. These results indicate that orally delivered PrPs were incorporated by microfold cells promptly after challenge and that macrophages might act as a transporter of incorporated PrPs from the Peyer's patches to other lymphoid tissues and the peripheral nervous system.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ajpath.2011.05.058
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21763679; ● Search Scopus @ Elsevier (PMID): 21763679; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ajpath.2011.05.058

(DOI: 10.1016/j.ajpath.2011.05.058, PubMed: 21763679) Koji Fujita, Yoshitaka Yamaguchi, Tsuyoshi Mori, Naomi Muramatsu, Takahito Miyamoto, Masashi Yano, Hironori Miyata, Akira Ootsuyama, Makoto Sawada, Haruo Matsuda, Ryuji Kaji and Suehiro Sakaguchi :
Effects of a Brain-Engraftable Microglial Cell Line Expressing Anti-Prion scFv Antibodies on Survival Times of Mice Infected with Scrapie Prions.,
Cellular and Molecular Neurobiology, Vol.31, No.7, 999-1008, 2011.

(要約): We first verified that a single chain Fv fragment against prion protein (anti-PrP scFv) was secreted by HEK293T cells and prevented prion replication in infected cells. We then stably expressed anti-PrP scFv in brain-engraftable murine microglial cells and intracerebrally injected these cells into mice before or after infection with prions. Interestingly, the injection before or at an early time point after infection attenuated the infection marginally but significantly prolonged survival times of the mice. These suggest that the ex vivo gene transfer of anti-PrP scFvs using brain-engraftable cells could be a possible immunotherapeutic approach against prion diseases.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10571-011-9696-z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21516351; ● Search Scopus @ Elsevier (PMID): 21516351; ● Search Scopus @ Elsevier (DOI): 10.1007/s10571-011-9696-z

(DOI: 10.1007/s10571-011-9696-z, PubMed: 21516351) Suehiro Sakaguchi, Daisuke Ishibashi and Haruo Matsuda :
Antibody-based immunotherapeutic attempts experimental animal models of prion diseases.,
Expert Opinion on Therapeutic Patents, Vol.19, No.7, 907-917, 2009.

(要約): There has been a dramatic decrease in the risk of transmission of bovine spongiform encephalopathy to humans. In contrast, the risk of human-to-human transmission of variant Creutzfeldt-Jakob disease (vCJD) via medical treatments became potentially high since 4 vCJD cases were reported to be possibly transmitted through blood transfusion in the UK. However, no treatments are yet available for curing prion diseases. Conversion of the normal prion protein, PrP(C), to the amyloidogenic PrP, PrP(Sc), plays a pivotal role in the pathogenesis. Recently, certain anti-PrP or anti-37/67-kDa laminin receptor (LRP/LR) antibodies were shown to have the potential to cure chronically infected cells, clearing PrP(Sc) from the cells. This has raised the possibility of antibody based-immunotherapy for prion diseases. This article aims to introduce and discuss the recently published attempts of immunotherapy in prion diseases. Bibliographic research was carried out using the PubMed database. Patent literature was searched using the UK Intellectual Property Office website. No satisfying consequences in animals could be detected with anti-PrP antibodies directly infused into the brains of animals by the intraventricular route or by anti-PrP or anti-LRP/LR single chain fragment antibodies directly delivered into the brain by virus vector-mediated gene transfer. This is probably because such delivery systems failed to deliver the antibodies to the neurons relevant for the treatments.
(キーワード): Animals / Antibodies / Cattle / Disease Models, Animal / Drug Delivery Systems / Gene Transfer Techniques / Humans / Immunotherapy / Patents as Topic / Prion Diseases / Prions
(出版サイトへのリンク): ● Publication site (DOI): 10.1517/13543770902988530
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19514955; ● Search Scopus @ Elsevier (PMID): 19514955; ● Search Scopus @ Elsevier (DOI): 10.1517/13543770902988530

(DOI: 10.1517/13543770902988530, PubMed: 19514955) Suehiro Sakaguchi :
Prospects for Preventative Vaccines against Prion Diseases,
Protein and Peptide Letters, Vol.16, No.3, 260-270, 2009.

(要約): Emergence of variant type of Creutzfeldt-Jakob disease (vCJD) in humans due to infection from bovine spongiform encephalopathy contaminated beef and recent reports of human-to-human transmission of vCJD via blood transfusion have raised great concern about an epidemic of vCJD. The disease is currently difficult to diagnose during pre-clinical stages and requires a very long incubation period for neurological symptoms to be evident. This therefore suggests that the disease is already latently spreading and that opportunity for infection is thus growing among human populations. Interestingly, passive immunization with antibodies against prion protein (PrP), a major component of the prion infectious agents, was shown to protect mice from infection, indicating the possibility of prion vaccines. However, PrP is a host protein therefore immune tolerance to PrP has hampered development of them. Here, the so far reported attempts to overcome the tolerance to elicit protective immunity to prions are briefly reviewed.
(キーワード): Animals / Humans / Immune Tolerance / Immunization / Prion Diseases / Prions / Vaccines
(出版サイトへのリンク): ● Publication site (DOI): 10.2174/092986609787601804
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19275739; ● Search Scopus @ Elsevier (PMID): 19275739; ● Search Scopus @ Elsevier (DOI): 10.2174/092986609787601804

(DOI: 10.2174/092986609787601804, PubMed: 19275739) Daisuke Yoshikawa, Naohiro Yamaguchi, Daisuke Ishibashi, Hitoki Yamanaka, Nobuhiko Okimura, Yoshitaka Yamaguti, Tsuyoshi Mori, Hironori Miyata, Kazuto Shigematsu, Shigeru Katamine and Suehiro Sakaguchi :
Dominant-negative effects of the N-terminal half of prion protein on neurotoxicity of prion protein-like protein/doppel in mice.,
The Journal of Biological Chemistry, Vol.283, No.35, 24202-24211, 2008.

(要約): Prion protein-like protein/doppel is neurotoxic, causing ataxia and Purkinje cell degeneration in mice, whereas prion protein antagonizes doppel-induced neurodegeneration. Doppel is homologous to the C-terminal half of prion protein but lacks the amino acid sequences corresponding to the N-terminal half of prion protein. We show here that transgenic mice expressing a fusion protein consisting of the N-terminal half, corresponding to residues 1-124, of prion protein and doppel in neurons failed to develop any neurological signs for up to 730 days in a background devoid of prion protein. In addition, the fusion protein prolonged the onset of ataxia in mice expressing exogenous doppel. These results suggested that the N-terminal part of prion protein has a neuroprotective potential acting both cis and trans on doppel. We also show that prion protein lacking the pre-octapeptide repeat (Delta25-50) or octapeptide repeat (Delta51-90) region alone could not impair the antagonistic function against doppel.
(キーワード): Animals / Ataxia / GPI-Linked Proteins / Humans / Mice / Mice, Transgenic / Neurodegenerative Diseases / Prions / Protein Structure, Tertiary / Purkinje Cells / Recombinant Fusion Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M804212200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18562311; ● Search Scopus @ Elsevier (PMID): 18562311; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M804212200

(DOI: 10.1074/jbc.M804212200, PubMed: 18562311) Suehiro Sakaguchi :
Antagonistic roles of the N-terminal domain of prion protein to doppel,
Prion, Vol.2, No.3, 107-111, 2008.

(要約): Prion protein (PrP)-like molecule, doppel (Dpl), is neurotoxic in mice, causing Purkinje cell degeneration. In contrast, PrP antagonizes Dpl in trans, rescuing mice from Purkinje cell death. We have previously shown that PrP with deletion of the N-terminal residues 23-88 failed to neutralize Dpl in mice, indicating that the N-terminal region, particularly that including residues 23-88, may have trans-protective activity against Dpl. Interestingly, PrP with deletion elongated to residues 121 or 134 in the N-terminal region was shown to be similarly neurotoxic to Dpl, indicating that the PrP C-terminal region may have toxicity which is normally prevented by the N-terminal domain in cis. We recently investigated further roles for the N-terminal region of PrP in antagonistic interactions with Dpl by producing three different types of transgenic mice. These mice expressed PrP with deletion of residues 25-50 or 51-90, or a fusion protein of the N-terminal region of PrP with Dpl. Here, we discuss a possible model for the antagonistic interaction between PrP and Dpl.
(キーワード): Amino Acid Motifs / Animals / GPI-Linked Proteins / Mice / Models, Biological / Neurotoxins / PrPC Proteins / Prion Diseases / Prions
(出版サイトへのリンク): ● Publication site (DOI): 10.4161/pri.2.3.7436
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19158506; ● Search Scopus @ Elsevier (PMID): 19158506; ● Search Scopus @ Elsevier (DOI): 10.4161/pri.2.3.7436

(DOI: 10.4161/pri.2.3.7436, PubMed: 19158506) Y Nasu-Nishimura, Y Taniuchi, T Nishimura, A Sakudo, K Nakajima, Y Ano, K Sugiura, Suehiro Sakaguchi, S Itohara and T Onodera :
Cellular prion protein prevents brain damage after encephalomyocarditis virus infection in mice,
Archives of Virology, Vol.153, No.6, 1007-1012, 2008.

(要約): Cellular prion protein (PrP(C)), a cell-surface glycoprotein normally associated with neurons, is also expressed in other cell types such as glia and lymphocytes. To further elucidate these roles of PrP(C), wild-type prion protein gene (Prnp(+/+)) mice and Prnp-deficient (Prnp(-/-)) mice were infected with encephalomyocarditis virus B variant (EMCV-B) via an intracranial route. EMCV-B causes encephalitis and apoptotic cell death in vivo. Histopathological studies revealed that Prnp(+/+) mice infected with 600 pfu of EMCV-B showed more severe infiltration of inflammatory cells, accompanied by higher activation of microglia cells around the hippocampus, than Prnp(-/-) mice; viz., no differences in the brain virus titer between these two lines of mice. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP, nick end-labeling (TUNEL) staining of the brain specimens revealed that the CA1 hippocampal pyramidal cells showed a larger number of apoptotic neurons in Prnp(-/-) than Prnp(+/+) mice. Based on all these findings, PrP(C) may play certain roles in the induction of inflammation and inhibition of apoptosis in vivo.
(キーワード): Animals / Apoptosis / Cardiovirus Infections / Cell Count / Encephalomyocarditis virus / Hippocampus / Inflammation / Mice / Mice, Knockout / Microglia / PrPC Proteins / Prions / Pyramidal Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00705-008-0086-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18408883; ● Search Scopus @ Elsevier (PMID): 18408883; ● Search Scopus @ Elsevier (DOI): 10.1007/s00705-008-0086-x

(DOI: 10.1007/s00705-008-0086-x, PubMed: 18408883) Suehiro Sakaguchi :
Recent developments in therapeutics for prion diseases,
Expert Opinion on Therapeutic Patents, Vol.18, No.1, 35-59, 2008.

(出版サイトへのリンク): ● Publication site (DOI): 10.1517/13543776.18.1.35
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1517/13543776.18.1.35

(DOI: 10.1517/13543776.18.1.35) Suehiro Sakaguchi :
Molecular biology of prion protein and its first homologous protein,
The Journal of Medical Investigation : JMI, Vol.54, No.3-4, 211-223, 2007.

(要約): Conformational conversion of the normal cellular isoform of prion protein, PrP(C), a glycoprotein anchored to the cell membrane by a glycosylphosphatidylinositol moiety, into the abnormally folded, amyloidogenic prion protein, PrP(Sc), plays a pivotal role in the pathogenesis of prion diseases. It has been suggested that PrP(C) might be functionally disturbed by constitutive conversion to PrP(Sc) due to either the resulting depletion of PrP(C) or the dominant negative effects of PrP(Sc) on PrP(C) or both. Consistent with this, we and others showed that mice devoid of PrP(C) (PrP-/-) spontaneously developed abnormal phenotypes very similar to the neurological abnormalities of prion diseases, supporting the concept that functional loss of PrP(C) might at least be partly involved in the pathogenesis of the diseases. However, no neuronal cell death could be detected in PrP-/- mice, indicating that the functional loss of PrP(C) alone might not be enough to induce neuronal cell death, one of major pathological hallmarks of prion diseases. Interestingly, it was recently shown that the first identified PrP-like protein, termed PrPLP/Doppel (Dpl), is neurotoxic in the absence of PrP(C), causing Purkinje cell degeneration in the cerebellum of mice. Although it is not understood if PrP(Sc) could have a neurotoxic potential similar to PrPLP/Dpl, it is very interesting to speculate that accumulation of PrP(Sc) and the functional disturbance of PrP(C), both of which are caused by constitutive conversion, might be required for the neurodegeneration in prion diseases.
(キーワード): Animals / Humans / Mice / Mice, Knockout / Molecular Biology / Nerve Degeneration / Neuroglia / Neurons / Prion Diseases / Prions / Purkinje Cells
(徳島大学機関リポジトリ): ● Metadata: 111516
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.54.211
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17878669; ● Search Scopus @ Elsevier (PMID): 17878669; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.54.211

(徳島大学機関リポジトリ: 111516, DOI: 10.2152/jmi.54.211, PubMed: 17878669) CK Kim, A Sakudo, Y Taniuchi, K Shigematsu, CB Kang, K Saeki, Y Matsumoto, Suehiro Sakaguchi, S Itohara and T Onodera :
Late-onset olfactory deficits and mitral cell loss in mice lacking prion protein with ectopic expression of Doppel,
International Journal of Molecular Medicine, Vol.20, No.2, 169-176, 2007.

(要約): Several lines of prion protein gene (Prnp)-knockout mice such as ZrchI, ZrchII, Npu, Ngsk and Rcm0 have been generated. Of these, ZrchII, Ngsk and Rcm0 exhibit late-onset ataxia due to ectopic expression of Doppel (Dpl); a result of damage to the splicing acceptor of Prnp exon 3. Recently, we developed another line of Prnp-/- mice (Rikn), which was generated by gene targeting with more nucleotides by replacing intron 2 with the pgk-neo gene (cf. Ngsk Prnp-/- mice) and showed not only ataxia but also a lower olfactory sensitivity than the other Prnp-/- mouse line ZrchI at over 60 weeks of age. The histopathology of the elderly Rikn Prnp-/- mice showed mitral cell loss concomitantly observed with gliosis of astrocytes. Western blot analysis showed that Dpl was detected in the cerebrum, cerebellum and olfactory bulb of Rikn Prnp-/- mice, where aberrant histopathology was observed. Thus, mitral cell loss and gliosis induced by ectopic Dpl expression were probably associated with the late-onset olfactory deficits in Rikn Prnp-/- mice.
(キーワード): Age of Onset / Aging / Animals / Brain / Caspase 3 / Cell Count / DNA, Single-Stranded / GPI-Linked Proteins / Gene Expression Regulation / Gliosis / Mice / Mice, Knockout / Olfaction Disorders / Olfactory Bulb / Prions / Tissue Distribution
(出版サイトへのリンク): ● Publication site (DOI): 10.3892/ijmm.20.2.169
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17611634; ● Summary page in Scopus @ Elsevier: 2-s2.0-35448993375

(DOI: 10.3892/ijmm.20.2.169, PubMed: 17611634, Elsevier: Scopus) CK Kim, Y Hirose, A Sakudo, N Takeyama, CB Kang, Y Taniuchi, Y Matsumoto, S Itohara, Suehiro Sakaguchi and T Onodera :
Reduced response of splenocytes after mitogen-stimulation in the prion protein (PrP) gene-deficient mouse: PrPLP/Doppel production and cerebral degeneration,
Biochemical and Biophysical Research Communications, Vol.358, No.2, 469-474, 2007.

(要約): Splenocytes of wild-type (Prnp(+/+)) and prion protein gene-deficient (Prnp(-/-)) mice were treated with various activation stimuli such as T cell mitogen concanavalin A (ConA), phorbol 12-myristate 13-acetate (PMA)+ionomycin (Io), or B cell mitogen lipopolysaccharide (LPS). Cellular prion protein (PrP(C)) expression was enhanced following ConA stimulation, but not PMA+Io or LPS in Prnp(+/+) splenocytes. Rikn Prnp(-/-) splenocytes elicited lower cell proliferations than Prnp(+/+) or Zrch I Prnp(-/-) splenocytes after LPS stimulation and showed sporadic nerve cells in the cerebral cortex and deeper structure. Around the degenerated nerve cells, mild vacuolation in the neuropil was observed. This neural alteration correlated well to the suppressed response of B cells in the spleen. The finding that discrete lesions within the central nervous systems induced marked modulation of immune function probably indicates the existence of a delicately balanced neural-endocrine network by PrP(C) and PrPLP/Doppel.
(キーワード): Animals / Cerebral Cortex / GPI-Linked Proteins / Lymphocytes / Mice / Mice, Knockout / Mitogens / Prions
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2007.04.174
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17498663; ● Search Scopus @ Elsevier (PMID): 17498663; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2007.04.174

(DOI: 10.1016/j.bbrc.2007.04.174, PubMed: 17498663) Jiaxin Dong, Aimin Li, Naohiro Yamaguchi, Suehiro Sakaguchi and David A. Harris :
Doppel induces degeneration of cerebellar Purkinje cells independently of Bax,
The American Journal of Pathology, Vol.171, No.2, 599-607, 2007.

(要約): Doppel (Dpl) is a prion protein paralog that causes neurodegeneration when expressed ectopically in the brain. To investigate the cellular mechanism underlying this effect, we analyzed Dpl-expressing transgenic mice in which the gene for the proapoptotic protein Bax had been deleted. We found that Bax deletion does not alter either clinical symptoms or Purkinje cell degeneration in Dpl transgenic mice. In addition, we observed that degenerating Purkinje cells in these animals do not display DNA fragmentation or caspase-3 activation. Our results suggest that non-Bax-dependent pathways mediate the toxic effects of Dpl in Purkinje cells, highlighting a possible role for nonapoptotic mechanisms in the death of these neurons.
(キーワード): Animals / Apoptosis / Blotting, Western / Caspase 3 / Cell Count / Cerebellum / DNA Fragmentation / Enzyme Activation / Female / GPI-Linked Proteins / Gene Deletion / Genotype / Male / Mice / Mice, Inbred C57BL / Mice, Inbred CBA / Mice, Knockout / Mice, Transgenic / Microscopy, Confocal / Nerve Degeneration / Phenotype / Prions / Purkinje Cells / Time Factors / bcl-2 Homologous Antagonist-Killer Protein / bcl-2-Associated X Protein
(出版サイトへのリンク): ● Publication site (DOI): 10.2353/ajpath.2007.070262
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17569776; ● Search Scopus @ Elsevier (PMID): 17569776; ● Search Scopus @ Elsevier (DOI): 10.2353/ajpath.2007.070262

(DOI: 10.2353/ajpath.2007.070262, PubMed: 17569776) Nishimura Takuya, Sakudo Akikazu, Hashiyama Yoriko, Yachi Akiko, Saeki Keiichi, Matsumoto Yoshitsugu, Ogawa Masaharu, Suehiro Sakaguchi, Itohara Shigeyoshi and Onodera Takashi :
Serum withdrawal-induced apoptosis in ZrchI prion protein (PrP) gene-deficient neuronal cell line is suppressed by PrP, independent of Doppel,
Microbiology and Immunology, Vol.51, No.4, 457-466, 2007.

(要約): Previous studies have shown that cellular prion protein (PrP(C)) plays anti-apoptotic and antioxidative role against cell death induced by serum-deprivation (SDP) in an immortalized prion protein gene-deficient neuronal cell line derived from Rikn prion protein (PrP) gene-deficient (Prnp(-/-)) mice, which ectopically produce excess Doppel (Dpl) (PrP-like glycoprotein). To investigate whether PrP(C) inhibits apoptotic neuronal cell death without Dpl, an immortalized cell line was established from the brain of ZrchI Prnp(-/-) mice, which do not show ectopic expression of Dpl. The results using a ZrchI neuronal Prnp(-/-) cell line (NpL2) showed that PrP(C) potently inhibited SDP-induced apoptotic cell death. Furthermore, PrP(C) expression enhanced the superoxide dismutase (SOD) activity in NpL2 cells. These results indicate that Dpl production did not affect anti-apoptotic and anti-oxidative functions of PrP, suggesting that PrP(C) may be directly correlated with protection against oxidative stress.
(キーワード): Animals / Apoptosis / GPI-Linked Proteins / Hippocampus / Mice / Mice, Knockout / Neurons / Prions / Protein Precursors / Signal Transduction
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17446686; ● Search Scopus @ Elsevier (PMID): 17446686

(PubMed: 17446686) K Miyazawa, T Kanaya, S Tanaka, I Takakura, K Watanabe, S Ohwada, H Kitazawa, MT Rose, Suehiro Sakaguchi, S Katamine, T Yamaguchi and H Aso :
Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice,
Histochemistry and Cell Biology, Vol.127, No.3, 291-301, 2007.

(要約): The gastrointestinal tract is thought to be the main site of entry for the pathological isoform of the prion protein (PrP(Sc)). Prion diseases are believed to result from a conformational change of the cellular prion protein (PrP(c)) to PrP(Sc). Therefore, PrP(c) expression is a prerequisite for the infection and spread of the disease to the central nervous system. However, the distribution of PrP(c) in the gut is still a matter of controversy. We therefore investigated the localization of PrP(c) in the bovine and murine small intestine. In cattle, most PrP(c) positive epithelial cells were detected in the duodenum, while a few positive cells were found in the jejunum. PrP(c) was expressed in serotonin producing cells. In bovine Peyer's patches, PrP(c) was distributed in extrafollicular areas, but not in the germinal centre of the jejunum and ileum. PrP(c) was expressed in myeloid lineage cells such as myeloid dendritic cells and macrophages. In mice, PrP(c) was expressed in some epithelial cells throughout the small intestine as well as in cells such as follicular dendritic cell in the germinal centre of Peyer's patches. In this study, we demonstrate that there are a number of differences in the localization of PrP(c) between the murine and bovine small intestines.
(キーワード): Animals / Cattle / Dendritic Cells / Epithelial Cells / Immunohistochemistry / Intestine, Small / Macrophages / Male / Mice / Mice, Inbred BALB C / Mice, Knockout / Peyer's Patches / PrPC Proteins / Prions
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00418-006-0250-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17165097; ● Search Scopus @ Elsevier (PMID): 17165097; ● Search Scopus @ Elsevier (DOI): 10.1007/s00418-006-0250-x

(DOI: 10.1007/s00418-006-0250-x, PubMed: 17165097) Suehiro Sakaguchi and Arakawa Takeshi :
Recent developments in mucosal vaccines against prion diseases,
Expert Review of Vaccines, Vol.6, No.1, 75-85, 2007.

(要約): Bovine spongiform encephalopathy in cattle is highly suspected to be orally transmitted to humans through contaminated food, causing new variant Creutzfeldt-Jakob disease. However, no prophylactic procedures against these diseases, such as vaccines, in particular those stimulating mucosal protective immunity, have been established. The causative agents of these diseases, termed prions, consist of the host-encoded prion protein (PrP). Therefore, prions are immunologically tolerated, inducing no host antibody responses. This immune tolerance to PrP has hampered the development of vaccines against prions. We and others recently reported that the immune tolerance could be successfully broken and mucosal immunity could be stimulated by mucosal immunization of mice with PrP fused with bacterial enterotoxin or delivered using an attenuated Salmonella strain, eliciting significantly higher immunoglobulin A and G antibody responses against PrP. In this review, we will discuss these reports.
(キーワード): Animals / Cattle / Encephalopathy, Bovine Spongiform / Humans / Immunity, Mucosal / Mice / Mucous Membrane / Prion Diseases / Prions / Technology, Pharmaceutical / Vaccines
(出版サイトへのリンク): ● Publication site (DOI): 10.1586/14760584.6.1.75
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17280480; ● Search Scopus @ Elsevier (PMID): 17280480; ● Search Scopus @ Elsevier (DOI): 10.1586/14760584.6.1.75

(DOI: 10.1586/14760584.6.1.75, PubMed: 17280480) Daisuke Ishibashi, Hitoki Yamanaka, Naohiro Yamaguchi, Daisuke Yoshikawa, Risa Nakamura, Nobuhiko Okimura, Yoshitaka Yamaguti, Kazuto Shigematsu, Shigeru Katamine and Suehiro Sakaguchi :
Immunization with recombinant bovine but not mouse prion protein delays the onset of disease in mice inoculated with a mouse-adapted prion,
Vaccine, Vol.25, No.6, 985-992, 2007.

(要約): Host tolerance to endogenous prion protein (PrP) has hampered the development of prion vaccines as PrP is a major component of prions. Indeed, we show that immunization of mice with mouse recombinant PrP elicited no prophylactic effect against a mouse-adapted prion. However, interestingly, mice immunized with recombinant bovine PrP developed the disease significantly later than non-immunized mice after inoculation of a mouse prion. Sheep recombinant PrP exhibited variable prophylactic effects. Mouse recombinant PrP stimulated only very weak antibody responses. In contrast, bovine recombinant PrP was higher immunogenic and produced variable amounts of anti-mouse PrP autoantibodies. Sheep recombinant PrP was also immunogenic but produced more variable amounts of anti-PrP autoantibodies. These results might open a new way for development of prion vaccines.
(キーワード): Animals / Autoantibodies / COS Cells / Cattle / Cercopithecus aethiops / Female / Immunization / Mice / Mice, Inbred BALB C / Molecular Mimicry / Prion Diseases / Prions / Recombinant Proteins / Sheep
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.vaccine.2006.09.078
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17055125; ● Search Scopus @ Elsevier (PMID): 17055125; ● Search Scopus @ Elsevier (DOI): 10.1016/j.vaccine.2006.09.078

(DOI: 10.1016/j.vaccine.2006.09.078, PubMed: 17055125) Yoshikawa Daisuke, Kopacek Juraj, Yamaguchi Naohiro, Ishibashi Daisuke, Yamanaka Hitoki, Yoshitaka Yamaguti, Katamine Shigeru and Suehiro Sakaguchi :
Newly established in vitro system with fluorescent proteins shows that abnormal expression of downstream prion protein-like protein in mice is probably due to functional disconnection between splicing and 3' formation of prion protein pre-mRNA,
Gene, Vol.386, No.1-2, 139-146, 2007.

(要約): We and others previously showed that, in some lines of prion protein (PrP)-knockout mice, the downstream PrP-like protein (PrPLP/Dpl) was abnormally expressed in brains partly due to impaired cleavage/polyadenylation of the residual PrP promoter-driven pre-mRNA despite the presence of a poly(A) signal. In this study, we newly established an in vitro transient transfection system in which abnormal expression of PrPLP/Dpl can be visualized by expression of the green fluorescence protein, EGFP, in cultured cells. No EGFP was detected in cells transfected by a vector carrying a PrP genomic fragment including the region targeted in the knockout mice intact upstream of the PrPLP/Dpl gene. In contrast, deletion of the targeted region from the vector caused expression of EGFP. By employing this system with other vectors carrying various deletions or point mutations in the targeted region, we identified that disruption of the splicing elements in the PrP terminal intron caused the expression of EGFP. Recent lines of evidence indicate that terminal intron splicing and cleavage/polyadenylation of pre-mRNA are functionally linked to each other. Taken together, our newly established system shows that the abnormal expression of PrPLP/Dpl in PrP-knockout mice caused by the impaired cleavage/polyadenylation of the PrP promoter-driven pre-mRNA is due to the functional dissociation between the pre-mRNA machineries, in particular those of cleavage/polyadenylation and splicing. Our newly established in vitro system, in which the functional dissociation between the pre-mRNA machineries can be visualized by EGFP green fluorescence, may be useful for studies of the functional connection of pre-mRNA machineries.
(キーワード): 3' Untranslated Regions / Animals / Cell Line, Tumor / GPI-Linked Proteins / Gene Expression Regulation / Green Fluorescent Proteins / Mice / Mice, Knockout / Polyadenylation / Prions / RNA Precursors / RNA Splicing
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.gene.2006.08.028
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17034959; ● Search Scopus @ Elsevier (PMID): 17034959; ● Search Scopus @ Elsevier (DOI): 10.1016/j.gene.2006.08.028

(DOI: 10.1016/j.gene.2006.08.028, PubMed: 17034959) Satoshi Kawatake, Yuki Nishimura, Suehiro Sakaguchi, Toru Iwaki and Katsumi Doh-ura :
Surface plasmon resonance analysis for the screening of ant-prion compounds.,
Biological & Pharmaceutical Bulletin, Vol.29, No.5, 927-932, 2006.

(要約): The interaction of anti-prion compounds and amyloid binding dyes with a carboxy-terminal domain of prion protein (PrP121-231) was examined using surface plasmon resonance (SPR) and compared with inhibition activities of abnormal PrP formation in scrapie-infected cells. Most examined compounds had affinities for PrP121-231: antimalarials had low affinities, whereas Congo red, phthalocyanine and thioflavin S had high affinities. The SPR binding response correlated with the inhibition activity of abnormal PrP formation. Several drugs were screened using SPR to verify the findings: propranolol was identified as a new anti-prion compound. This fact indicates that drug screenings by this assay are useful.
(キーワード): Animals / Anti-Infective Agents / Antimalarials / Cell Line / Drug Evaluation, Preclinical / Kinetics / Mice / Prions / Protein Binding / Recombinant Proteins / Scrapie / Surface Plasmon Resonance
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/bpb.29.927
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16651721; ● Search Scopus @ Elsevier (PMID): 16651721; ● Search Scopus @ Elsevier (DOI): 10.1248/bpb.29.927

(DOI: 10.1248/bpb.29.927, PubMed: 16651721) Hitoki Yamanaka, Daisuke Ishibashi, Naohiro Yamaguchi, Daisuke Yoshikawa, Risa Nakamura, Nobuhiko Okimura, Takeshi Arakawa, Takao Tsuji, Shigeru Katamine and Suehiro Sakaguchi :
Enhanced mucosal immunogenicity of prion protein following fusion with B subunit of Escherichia coli heat-labile enterotoxin.,
Vaccine, Vol.24, No.15, 2815-2823, 2006.

(要約): Mucosal vaccine against prion protein (PrP), a major component of prions, is urgently awaited since the oral transmission of prions from cattle to humans is highly suspected. In the present study, we produced recombinant bovine and mouse PrPs fused with or without the B subunit of Escherichia coli heat-labile enterotoxin (LTB) and intranasally immunized mice with these fused proteins. Fusion with LTB markedly enhanced the mucosal immunogenicity of bovine PrP, producing a marked increase in specific IgG and IgA titer in serum. Mouse PrP also showed slightly increased immunogenicity following fusion with LTB. These results demonstrate that LTB-fused PrPs might be potential candidates for protective mucosal prion vaccines.
(キーワード): Administration, Intranasal / Animals / Antibodies / Bacterial Toxins / Enterotoxins / Escherichia coli / Escherichia coli Proteins / Female / Immunity, Mucosal / Immunoglobulin A / Immunoglobulin G / Mice / Mice, Inbred BALB C / Mice, Inbred C57BL / Prions / Recombinant Fusion Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.vaccine.2005.12.054
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16446015; ● Search Scopus @ Elsevier (PMID): 16446015; ● Search Scopus @ Elsevier (DOI): 10.1016/j.vaccine.2005.12.054

(DOI: 10.1016/j.vaccine.2005.12.054, PubMed: 16446015) Yasuko Sakurai-Yamashita, Suehiro Sakaguchi, Daisuke Yoshikawa, Nobuhiko Okimura, Yoshiko Masuda, Shigeru Katamine and Masami Niwa :
Female-specific neuroprotection against transient brain ischemia observed in mice devoid of prion protein is abolished by ectopic expression of prion protein-like protein.,
Neuroscience, Vol.136, No.1, 281-287, 2005.

(要約): This study was designed to examine the function of cellular prion protein and prion protein-like protein/Doppel, in transient ischemia-related neuronal death in the hippocampus. Two different lines of mice devoid of cellular prion protein, Zrch I Prnp(0/0) and Ngsk Prnp(0/0), were used. The former lacks cellular prion protein whereas the latter ectopically expresses prion protein-like protein/Doppel in the brain in the absence of cellular prion protein. Mice were subjected to 10 min-occlusion of the bilateral common carotid arteries with recovery for 14 days. Less than 10% of the pyramidal neurons in the CA1 subfield were degenerated in male and female wild-type mice. In contrast, more than half of the neurons were lost in male Zrch I Prnp(0/0) and Ngsk Prnp(0/0) mice. Such severe neuronal loss was also observed in female Ngsk Prnp(0/0) mice. However, female Zrch I Prnp(0/0) mice showed mild neuronal loss similar to wild-type mice. Flunarizine, a T- and L-type Ca(2+)-channel antagonist, significantly reduced the neuronal loss in female but not in male Ngsk Prnp(0/0) mice. These results indicate that loss of cellular prion protein renders hippocampal neurons susceptible to ischemic insult specifically in male but not female mice and the ectopic expression of prion protein-like protein/Doppel aggravates the ischemic neuronal death in female prion protein-null mice probably via overloading of Ca(2+)-dependent signaling.
(キーワード): Amyloid / Animals / Brain / Calcium Channel Blockers / Cell Death / Estradiol / Female / Flunarizine / GPI-Linked Proteins / Hippocampus / In Situ Nick-End Labeling / Ischemic Attack, Transient / Male / Mice / Mice, Inbred C57BL / Mice, Knockout / Neurons / Neuroprotective Agents / Prions / Protein Precursors / Sex Characteristics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.neuroscience.2005.06.095
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16198494; ● Search Scopus @ Elsevier (PMID): 16198494; ● Search Scopus @ Elsevier (DOI): 10.1016/j.neuroscience.2005.06.095

(DOI: 10.1016/j.neuroscience.2005.06.095, PubMed: 16198494) Kazuhiko Arima, Noriyuki Nishida, Suehiro Sakaguchi, Kazuto Shigematsu, Ryuichiro Atarashi, Naohiro Yamaguchi, Daisuke Yoshikawa, Jaewoo Yoon, Ken Watanabe, Nobuyuki Kobayashi, Sophie Mouillet-Richard, Sylvain Lehmann and Shigeru Katamine :
Biological and biochemical characteristics of prion strains conserved in persistently-infected cell cultures.,
Journal of Virology, Vol.79, No.11, 7104-7112, 2005.

(要約): Abnormal prion protein (PrP(Sc)) plays a central role in the transmission of prion diseases, but the molecular basis of prion strains with distinct biological characteristics remains to be elucidated. We analyzed the characteristics of prion disease by using mice inoculated with the Chandler and Fukuoka-1 strains propagated in a cultured mouse neuronal cell line, GT1-7, which is highly permissive to replication of the infectious agents. Strain-specific biological characteristics, including clinical manifestations, incubation period as related to the infectious unit, and pathological profiles, remained unchanged after passages in the cell cultures. We noted some differences in the biochemical aspects of PrP(Sc) between brain tissues and GT1-7 cells which were unlikely to affect the strain phenotypes. On the other hand, the proteinase K-resistant PrP core fragments derived from Fukuoka-1-infected tissues and cells were slightly larger than those from Chandler-infected versions. Moreover, Fukuoka-1 infection, but not Chandler infection, gave an extra fragment with a low molecular weight, approximately 13 kDa, in both brain tissues and GT1-7 cells. This cell culture model persistently infected with different strains will provide a new insight into the understanding of the molecular basis of prion diversity.
(キーワード): Animals / Brain / Brain Chemistry / Cell Line / Humans / Mice / Neurons / PrPSc Proteins / Prion Diseases / Prions / Species Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/JVI.79.11.7104-7112.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15890950; ● Search Scopus @ Elsevier (PMID): 15890950; ● Search Scopus @ Elsevier (DOI): 10.1128/JVI.79.11.7104-7112.2005

(DOI: 10.1128/JVI.79.11.7104-7112.2005, PubMed: 15890950) Suehiro Sakaguchi :
Prion protein, prion protein-like protein, and neurodegeneration.,
Neurodegeneration and Prion Disease edited by David R. Brown, 167-193, 2005.

(出版サイトへのリンク): ● Publication site (DOI): 10.1007/0-387-23923-5_7
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-58149471049

(DOI: 10.1007/0-387-23923-5_7, Elsevier: Scopus) Takuya Nishimura, Akikazu Sakudo, Izuru Nakamura, Deug-chan Lee, Yoichiro Taniuchi, Keiichi Saeki, Yoshitsugu Matsumoto, Masaharu Ogawa, Suehiro Sakaguchi, Shigeyoshi Itohara and Takashi Onodera :
Cellular prion protein regulates intracellular hydrogen peroxide level and prevents copper-induced apoptosis.,
Biochemical and Biophysical Research Communications, Vol.323, No.1, 218-222, 2004.

(要約): The function of cellular prion protein (PrPC), which is a copper binding protein, remains unclear. To elucidate the mechanisms in which PrPC is involved in neuroprotection, we compared death signals in prion protein gene-deficient (Prnp-/-) primary cerebellar granular neurons (CGNs) to those with wild-type (WT) CGNs. When copper was exposed to these CGNs, ZrchI, and Rikn Prnp-/- CGNs were more sensitized and underwent apoptotic cell death more readily than WT CGNs. Furthermore, the level of intracellular hydrogen peroxide (H2O2) in WT CGNs increased by copper toxicity, whereas those in ZrchI and Rikn Prnp-/- CGNs did not. These results suggest that PrPC modulates the intracellular H2O2 level as a copper-binding protein to protect CGNs from apoptotic cell death possibly due to inhibiting a Fenton reaction.
(キーワード): Animals / Apoptosis / Cell Nucleus / Cell Survival / Cerebellum / Copper / Dose-Response Relationship, Drug / Flow Cytometry / Hydrogen Peroxide / Mice / Mice, Inbred C57BL / Neurons / Prions / Protein Binding / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2004.08.087
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15351724; ● Search Scopus @ Elsevier (PMID): 15351724; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2004.08.087

(DOI: 10.1016/j.bbrc.2004.08.087, PubMed: 15351724) Naohiro Yamaguchi, Suehiro Sakaguchi, Kazuto Shigematsu, Nobuhiko Okimura and Shigeru Katamine :
Doppel-induced Purkinje cell death is stoichiometrically abrogated by prion protein.,
Biochemical and Biophysical Research Communications, Vol.319, No.4, 1247-1252, 2004.

(要約): Mice devoid of prion protein (PrP) exhibiting ataxia and Purkinje cell degeneration, such as Ngsk Prnp(-/-) mice, ectopically express PrP-like protein, Dpl, in neurons including Purkinje cells. In this study, two types of transgenic (tg) mice expressing Dpl in neurons, tg(N-Dpl), or Purkinje cells only, tg(P-Dpl), were generated on the background of non-ataxic Zrch I Prnp(-/-) mice. In contrast to the tg mice with the Prnp(+/+) background, both tg mice with the Prnp(-/-) alleles developed Purkinje cell degeneration after incubation periods inversely correlated to the levels of Dpl. Some tg mice hemizygous for Prnp allele also developed disease but much later than those carrying the Prnp(-/-) alleles. This indicates that Dpl expressed by Purkinje cells itself is toxic to the cells, and that the neurotoxicity is stoichiometrically antagonized by PrP.
(キーワード): Amyloid / Animals / Ataxia / Brain / Cell Death / GPI-Linked Proteins / Genotype / In Situ Hybridization / Mice / Mice, Knockout / Mice, Transgenic / Phenotype / Prions / Protein Precursors / Purkinje Cells / Transgenes
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2004.05.115
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15194501; ● CiNii @ 国立情報学研究所 (CRID): 1360011145119574272; ● Search Scopus @ Elsevier (PMID): 15194501; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2004.05.115

(DOI: 10.1016/j.bbrc.2004.05.115, PubMed: 15194501, CiNii: 1360011145119574272) Akikazu Sakudo, Deug-chan Lee, Etsuro Yoshimura, Seiji Nagasaka, Kayako Nitta, Keiichi Saeki, Yoshitsugu Matsumoto, Sylvain Lehmann, Shigeyoshi Itohara, Suehiro Sakaguchi and Takashi Onodera :
Prion protein suppresses perturbation of cellular copper homeostasis under oxidative conditions.,
Biochemical and Biophysical Research Communications, Vol.313, No.4, 850-855, 2004.

(要約): Prion protein (PrP) binds copper and exhibits superoxide dismutase-like activity, while the roles of PrP in copper homeostasis remain controversial. Using Zeeman graphite furnace atomic absorption spectroscopy, we quantified copper levels in immortalized PrP gene (Prnp)-deficient neuronal cells transfected with Prnp and/or Prnd, which encodes PrP-like protein (PrPLP/Dpl), in the presence or absence of oxidative stress induced by serum deprivation. In the presence of serum, copper levels were not significantly affected by the expression of PrP and/or PrPLP/Dpl, whereas serum deprivation induced a decrease in copper levels that was inhibited by PrP but not by PrPLP/Dpl. The inhibitory effect of PrP on the decrease of copper levels was prevented by overexpression of PrPLP/Dpl. These findings indicate that PrP specifically stabilizes copper homeostasis, which is perturbed under oxidative conditions, while PrPLP/Dpl overexpression prevents PrP function in copper homeostasis, suggesting an interaction of PrP and PrPLP/Dpl and distinct functions between PrP and PrPLP/Dpl on metal homeostasis. Taken together, these results strongly suggest that PrP, in addition to its antioxidant properties, plays a role in stabilizing cellular copper homeostasis under oxidative conditions.
(キーワード): Animals / Antioxidants / Cell Line / Copper / GPI-Linked Proteins / Homeostasis / Male / Manganese / Mice / Mice, Knockout / Oxidation-Reduction / PrPC Proteins / Prions / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2003.12.020
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14706620; ● Search Scopus @ Elsevier (PMID): 14706620; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2003.12.020

(DOI: 10.1016/j.bbrc.2003.12.020, PubMed: 14706620) Suehiro Sakaguchi :
Antagonistic roles of prion protein and prion protein-like protein in neurodegeneration.,
Recent Research Developments in Experimental Medicine, Vol.1, 47-61, 2004. Ryuichiro Atarashi, Suehiro Sakaguchi, Kazuto Shigematsu and Shigeru Katamine :
The absence of prion-like infectivity in mice expressing prion protein-like protein.,
EXCLI Journal, Vol.3, 82-90, 2004. Masahisa Watarai, Suk Kim, Janchivdorj Erdenebaatar, Sou-ichi Makino, Motohiro Horiuchi, Toshikazu Shirahata, Suehiro Sakaguchi and Shigeru Katamine :
Cellular prion protein promotes Brucella infection into macrophages.,
The Journal of Experimental Medicine, Vol.198, No.1, 5-17, 2003.

(要約): The products of the Brucella abortus virB gene locus, which are highly similar to conjugative DNA transfer system, enable the bacterium to replicate within macrophage vacuoles. The replicative phagosome is thought to be established by the interaction of a substrate of the VirB complex with macrophages, although the substrate and its host cellular target have not yet been identified. We report here that Hsp60, a member of the GroEL family of chaperonins, of B. abortus is capable of interacting directly or indirectly with cellular prion protein (PrPC) on host cells. Aggregation of PrPC tail-like formation was observed during bacterial swimming internalization into macrophages and PrPC was selectively incorporated into macropinosomes containing B. abortus. Hsp60 reacted strongly with serum from human brucellosis patients and was exposed on the bacterial surface via a VirB complex-associated process. Under in vitro and in vivo conditions, Hsp60 of B. abortus bound to PrPC. Hsp60 of B. abortus, expressed on the surface of Lactococcus lactis, promoted the aggregation of PrPC but not PrPC tail formation on macrophages. The PrPC deficiency prevented swimming internalization and intracellular replication of B. abortus, with the result that phagosomes bearing the bacteria were targeted into the endocytic network. These results indicate that signal transduction induced by the interaction between bacterial Hsp60 and PrPC on macrophages contributes to the establishment of B. abortus infection.
(キーワード): Adaptor Proteins, Signal Transducing / Animals / Bacterial Proteins / Brucella abortus / Cells, Cultured / Chaperonin 60 / Female / GRB2 Adaptor Protein / Macrophages / Mice / Mice, Inbred BALB C / Mice, Inbred C57BL / Phenotype / Prions / Proteins / Signal Transduction
(出版サイトへのリンク): ● Publication site (DOI): 10.1084/jem.20021980
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12847134; ● Search Scopus @ Elsevier (PMID): 12847134; ● Search Scopus @ Elsevier (DOI): 10.1084/jem.20021980

(DOI: 10.1084/jem.20021980, PubMed: 12847134) Ryuichiro Atarashi, Noriyuki Nishida, Kazuto Shigematsu, Shinji Goto, Takahito Kondo, Suehiro Sakaguchi and Shigeru Katamine :
Deletion of N-terminal residues 23-88 from prion protein (PrP) abrogates the potential to rescue PrP-deficient mice from PrP-like protein/Doppel-induced neurodegeneration.,
The Journal of Biological Chemistry, Vol.278, No.31, 28944-28949, 2003.

(要約): Accumulating evidence has suggested that prion protein (PrP) is neuroprotective and that a PrP-like protein/Doppel (PrPLP/Dpl) is neurotoxic. A line of PrP-deficient mice, Ngsk Prnp0/0, ectopically expressing PrPLP/Dpl in neurons, exhibits late-onset ataxia because of Purkinje cell death that is prevented by a transgene encoding wild-type mouse PrP. To elucidate the mechanisms of neurodegeneration in these mice, we introduced five types of PrP transgene, namely one heterologous hamster, two mouse/hamster chimeric genes, and two mutants, each of which encoded PrP lacking residues 23-88 (MHM2.del23-88) or with E199K substitution (Mo.E199K), into Ngsk Prnp0/0 mice. Only MHM2.del23-88 failed to rescue the mice from the Purkinje cell death. The transgenic mice, MHM2.del23-88/Ngsk Prnp0/0, expressed several times more PrP than did wild-type (Prnp+/+) mice and PrPLP/Dpl at an equivalent level to Ngsk Prnp0/0 mice. Little difference was observed in the pathology and onset of ataxia between Ngsk Prnp0/0 and MHM2.del23-88/Ngsk Prnp0/0. No detergent-insoluble PrPLP/Dpl was detectable in the central nervous system of Ngsk Prnp0/0 mice even after the onset of ataxia. Our findings provide evidence that the N-terminal residues 23-88 of PrP containing the unique octapeptide-repeat region is crucial for preventing Purkinje cell death in Prnp0/0 mice expressing PrPLP/Dpl in the neuron.
(キーワード): Animals / Ataxia / Brain / Calcium-Binding Protein, Vitamin D-Dependent / Cell Death / Cricetinae / Detergents / GPI-Linked Proteins / Gene Deletion / Gene Expression / Glutathione Peroxidase / Mice / Mice, Transgenic / Nerve Degeneration / Neurons / Peptide Fragments / Phenotype / Pregnancy Proteins / Prions / Purkinje Cells / Repetitive Sequences, Nucleic Acid / Solubility / Superoxide Dismutase
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M303655200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12759361; ● Search Scopus @ Elsevier (PMID): 12759361; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M303655200

(DOI: 10.1074/jbc.M303655200, PubMed: 12759361) Ryuichiro Atarashi, Suehiro Sakaguchi, Kazuto Shigematsu, Kazuhiko Arima, Nobuhiko Okimura, Naohiro Yamaguchi, A Li, Juraj Kopacek and Shigeru Katamine :
Abnormal activation of glial cells in the brains of prion protein-deficient mice ectopically expressing prion protein-like protein, PrPLP/Dpl.,
Molecular Medicine, Vol.7, No.12, 803-809, 2001.

(要約): Some lines of mice homozygous for a disrupted prion protein gene (Prnp), including Ngsk Prnp(0/0) mice, exhibit Purkinje cell degeneration as a consequence of the ectopic overexpression of the downstream gene for prion protein-like protein (PrPLP/Dpl) in the brain, but others, such as Zrch I Prnp(0/0) mice, show neither the neurodegeneration nor the expression of PrPLP/Dpl. In the present study, we found that Ngsk Prnp(0/0), but not Zrch I Prnp(0/0) mice, developed gliosis involving both astrocytes and microglia in the brain. The brains from wild-type (Prnp(+/+)), Ngsk Prnp(0/0), Zrch I Prnp(0/0), and reconstituted Ngsk Prnp(0/0) mice carrying a mouse PrP transgene, designated Tg(P) Ngsk Prnp(0/0) mice, were subjected into Northern blotting and in situ hybridization using probes of glial fibrillary acidic protein (GFAP) and lysozyme M (LM) specific for astrocytes and microglia, respectively. Immunohistochemistry was also performed on the brain sections using anti-GFAP and anti-F4/80 antibodies. Northern blotting demonstrated upregulated expression of the genes for GFAP and LM in the brains of Ngsk Prnp(0/0), but not in Zrch I Prnp(0/0) mice. A transgene for normal mouse PrP(C) successfully rescued Ngsk Prnp(0/0) mice from the glial activation. In situ hybridization and immunohistochemistry revealed activated astrocytes and microglia mainly in the white matter of both the forebrains and cerebella. In contrast, there was no evidence of neuronal injury except for the Purkinje cell degeneration. Moreover, the glial cell activation was notable well before the onset of the Purkinje cell degeneration. These findings strongly suggest that ectopic PrPLP/Dpl in the absence of PrP(C) is actively involved in the glial-cell activation in the brain.
(キーワード): Aging / Animals / Astrocytes / Brain / GPI-Linked Proteins / Glial Fibrillary Acidic Protein / Gliosis / Mice / Muramidase / Nerve Degeneration / Neuroglia / PrPC Proteins / Prions / Purkinje Cells / RNA, Messenger / Recombinant Proteins / Transgenes / Up-Regulation
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11844868; ● Search Scopus @ Elsevier (PMID): 11844868

(PubMed: 11844868) Paolo Valenti, Antonio Cozzio, Noriyuki Nishida, David P. Wolfer, Suehiro Sakaguchi and Hans-Peter Lipp :
Similar target, different effects: late-onset ataxia and spatial learning in prion protein-deficient mouse lines.,
Neurogenetics, Vol.3, No.4, 173-184, 2001.

(要約): Several lines of mice with targeted deletion of the prion protein gene (Prnp) have been produced, some of them appearing phenotypically normal, others developing late-onset ataxia. This has been tentatively attributed to the size of the targeted deletion in the Prnp gene. but a masking role of genetic background could not be excluded. Thus, we have crossed an ataxic mutant line with large deletion of Prnp (Ngsk Prnp0/0) with a knockout line showing only partial deletion of Prnp and no neurological deficits (Zrchl Prnp0/0). A F2 generation was then studied for up to 70 weeks for co-segregation of lesion size and behavioral phenotype, including cognitive and neurological anomalies. These mice were later crossed with a recently generated PrP-deficient line also having a large deletion and late-onset ataxia (Zrch2 Prnp0/0). They underwent similar testing for up to 90 weeks. The ataxic phenotype always co-segregates with large homozygous deletions involving either the Ngsk or the Zrch2 allele, independent of genetic background or sex. Compound heterozygous Zrchl/Ngsk mice or Zrch1/Zrch2 mice showed intermediate neurological phenotypes, suggesting a gene-dosage effect of large deletions. At 12 weeks of age, large deletions were also associated with minor non-cognitive impairments in water maze learning, and hyperactivity in open field and elevated zero maze. These impairments were not predictive for the development of ataxia. Thus, the neurological deficits are closely associated with large deletions, which entail an upregulation of the recently discovered prion Doppel protein (Dpl), while genetic background factors seem to be responsible for shifting the onset of neurological symptoms.
(キーワード): Age of Onset / Animals / Ataxia / Behavior, Animal / Exploratory Behavior / Female / Gene Deletion / Gene Dosage / Male / Maze Learning / Memory / Mice / Mice, Inbred C57BL / Mice, Knockout / Phenotype / Prions / Space Perception
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s100480100117
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11714097; ● Search Scopus @ Elsevier (PMID): 11714097; ● Search Scopus @ Elsevier (DOI): 10.1007/s100480100117

(DOI: 10.1007/s100480100117, PubMed: 11714097) Ryota Nakaoke, Suehiro Sakaguchi, Ryuichiro Atarashi, Noriuki Nishida, Kazuhiko Arima, Kazuto Shigematsu and Shigeru Katamine :
Early appearance but lagged accumulation of detergent-insoluble prion protein in the brains of mice inoculated with a mouse-adapted Creutzfeldt-Jakob disease agent.,
Cellular and Molecular Neurobiology, Vol.20, No.6, 717-730, 2000.

(要約): 1. To elucidate mechanisms for the generation of the detergent-insoluble, proteinase K-resistant prion protein (PrP(Sc)) from the detergent-soluble, proteinase K-sensitive PrP (PrP(C)) and the replication of the infectious agent in prion diseases, we followed the kinetics of detergent-insoluble PrP and PrP(Sc) levels, infectious titers, and associated pathological changes in the brains of mice inoculated with a mouse-adapted Creutzfeldt Jakob disease agent. 2. PrP(Sc) in brain homogenate and detergent-insoluble PrP enriched by two-cycle ultracentrifugation were detected by immunoblotting and their relative amounts were estimated according to a standard curve plotted between the amount of PrP and signal intensity on immunoblotting. The titer of infectivity was determined by the incubation periods of mice inoculated with the unfractionated homogenate on the basis of a standard curve plotted between the titer and incubation period. 3. Detergent-insoluble PrP became detectable 4 weeks postinoculation (p.i.) well before the detection of PrP(Sc). The low level of detergent-insoluble PrP continued until dramatic accumulation occurred at 14 weeks p.i., correlating well with the accumulation of PrP(Sc) and development of pathological changes. The infectious titer was undetectable at 4 weeks p.i. and its logarithmic increase occurred 10 weeks p.i. preceding the logarithmic accumulation of PrPs. 4. The lag time of detergent-insoluble PrP accumulation and the discrepancy between infectious titers and PrPs observed during the early period after inoculation suggest a slow and rate-limiting step for the detergent-insoluble PrP to become the infectious agent-associated PrP(Sc).
(キーワード): Animals / Brain / Creutzfeldt-Jakob Syndrome / Disease Models, Animal / Endopeptidase K / Immunoblotting / Kinetics / Mice / Mice, Inbred C57BL / Mice, Inbred Strains / PrPSc Proteins / Prions / Ultracentrifugation
(出版サイトへのリンク): ● Publication site (DOI): 10.1023/A:1007054909662
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11100979; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033711734

(DOI: 10.1023/A:1007054909662, PubMed: 11100979, Elsevier: Scopus) Aimin Li, Suehiro Sakaguchi, Kazuto Shigematsu, Ryuichiro Atarashi, Bhabesh C. Roy, Ryota Nakaoke, Kazuhiko Arima, Nobuhiko Okimura, Juraj Kopacek and Shigeru Katamine :
Physiological expression of the gene for PrP-like protein, PrPLP/Dpl, by brain endothelial cells and its ectopic expression in neurons of PrP-deficient mice ataxic due to Purkinje cell degeneration.,
The American Journal of Pathology, Vol.157, No.5, 1447-1452, 2000.

(要約): Recently, a novel gene encoding a prion protein (PrP)-like glycoprotein, PrPLP/Dpl, was identified as being expressed ectopically by neurons of the ataxic PrP-deficient (PRNP(-/-)) mouse lines exhibiting Purkinje cell degeneration. In adult wild-type mice, PrPLP/Dpl mRNA was physiologically expressed at a high level by testis and heart, but was barely detectable in brain. However, transient expression of PrPLP/Dpl mRNA was detectable by Northern blotting in the brain of neonatal wild-type mice, showing maximal expression around 1 week after birth. In situ hybridization paired with immunohistochemistry using anti-factor VIII serum identified brain endothelial cells as expressing the transcripts. Moreover, in the neonatal wild-type mice, the PrPLP/Dpl mRNA colocalized with factor VIII immunoreactivities in spleen and was detectable on capillaries in lamina propria mucosa of gut. These findings suggested a role of PrPLP/Dpl in angiogenesis, in particular blood-brain barrier maturation in the central nervous system. Even in the ataxic Ngsk PRNP(-/-) mice, the physiological regulation of PrPLP/Dpl mRNA expression in brain endothelial cells was still preserved. This strongly supports the argument that the ectopic expression of PrPLP/Dpl in neurons, but not deregulation of its physiological expression in endothelial cells, is involved in the neuronal degeneration in ataxic PRNP(-/-) mice.
(キーワード): Adenosine Triphosphatases / Animals / Ataxia / Cerebrovascular Circulation / Endothelium, Vascular / Fungal Proteins / GPI-Linked Proteins / Gene Expression / Mice / Mice, Mutant Strains / Nerve Degeneration / Neurons / Prions / Purkinje Cells / RNA Helicases / Saccharomyces cerevisiae Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0002-9440(10)64782-7
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11073804; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033678443

(DOI: 10.1016/S0002-9440(10)64782-7, PubMed: 11073804, Elsevier: Scopus) Aimin Li, Suehiro Sakaguchi, Ryuichiro Atarashi, Bhabesh C. Roy, Ryota Nakaoke, Kazuhiko Arima, Nobuhiko Okimura, Juraj Kopacek and Kazuto Shigematsu :
Identification of a novel gene encoding a PrP-like protein expressed as chimeric transcripts fused to PrP exon 1/2 in ataxic mouse line with a disrupted PrP gene.,
Cellular and Molecular Neurobiology, Vol.20, No.5, 553-567, 2000.

(要約): 1. Mouse lines lacking prion protein (PrP(C)) have a puzzling phenotypic discrepancy. Some, but not all, developed late-onset ataxia due to Purkinje cell degeneration. 2. Here, we identified aberrant mRNA species in the brain of Ngsk Prnp0/0 ataxic, but not in nonataxic Zrch Prnp0/0 mouse line. These mRNAs were chimeric between the noncoding exons 1 and 2 of the PrP gene (Prnp) and the novel sequence encoding PrP-like protein (PrPLP), a putative membrane glycoprotein with 23% identity to PrP(C) in the primary amino acid structure. The chimeric mRNAs were generated from the disrupted Prnp locus of Ngsk Prnp0/0 mice lacking a part of the Prnp intron 2 and its splice acceptor signal. 3. In the brain of wild-type and Zrch Prnp0/0 mice, PrPLP mRNA was barely detectable. In contrast, in the brain of Ngsk Prnp0/0 mice, PrP/PrPLP chimeric mRNAs were expressed in neurons, at a particularly high level in hippocampus pyramidal cells and Purkinje cells under the control of the Prnp promoter. 4. In addition to the functional loss of PrP(C), ectopic PrPLP expression from the chimeric mRNAs could also be involved in the Purkinje cell degeneration in Ngsk Prnp0/0 mice.
(キーワード): Amino Acid Sequence / Animals / Ataxia / Base Sequence / Blotting, Northern / Brain Chemistry / Exons / GPI-Linked Proteins / Gene Expression / Introns / Mice / Mice, Knockout / Mice, Neurologic Mutants / Molecular Sequence Data / Phenotype / PrPC Proteins / Prions / Promoter Regions, Genetic / Purkinje Cells / RNA, Messenger / Recombinant Fusion Proteins / Transcription, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1023/A:1007059827541
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10930132; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033942010

(DOI: 10.1023/A:1007059827541, PubMed: 10930132, Elsevier: Scopus) Juraj Kopacek, Suehiro Sakaguchi, Kazuto Shigematsu, Noriyuki Nishida, Ryuichiro Atarashi, Ryota Nakaoke, Ryozo Moriuchi, Masami Niwa and Shigeru Katamine :
Upregulation of the genes encoding lysosomal hydrolases, a perforin-like protein, and peroxidases in the brains of mice affected with an experimental prion disease.,
Journal of Virology, Vol.74, No.1, 411-417, 2000.

(要約): In an attempt to identify the molecules involved in the pathogenesis of prion diseases, we performed cDNA subtraction on the brain tissues of mice affected with an experimental prion disease and the unaffected control. The genes identified as being upregulated in the prion-affected brain tissue included those encoding a series of lysosomal hydrolases (lysozyme M and both isoforms of beta-N-acetylhexosaminidase), a perforin-like protein (macrophage proliferation-specific gene-1 [MPS-1]), and an oxygen radical scavenger (peroxiredoxin). Dramatic increases in the expression level occurred at between 12 and 16 weeks after intracerebral inoculation of the prion, coinciding with the onset of spongiform degeneration. The proteinase K-resistant prion protein (PrP(Sc)) became detectable by immunoblotting well before 12 weeks, suggesting a causal relationship between this and the gene activation. Immunohistochemistry paired with in situ hybridization on sections of the affected brain tissue revealed that expression of the peroxiredoxin gene was detectable only in astrocytes and was noted throughout the affected brain tissue. On the other hand, the genes for the lysosomal hydrolases and MPS-1 were overexpressed exclusively by microglia, which colocalized with the spongiform morphological changes. A crucial role for microglia in the spongiform degeneration by their production of neurotoxic substances, and possibly via the aberrant activation of the lysosomal system, would have to be considered.
(キーワード): Animals / Brain / Brain Diseases / Hydrolases / Lysosomes / Membrane Glycoproteins / Mice / Perforin / Peroxidases / Pore Forming Cytotoxic Proteins / Prion Diseases / Up-Regulation
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10590130; ● Summary page in Scopus @ Elsevier: 2-s2.0-0033987067

(PubMed: 10590130, Elsevier: Scopus) Maria A. Deli, Suehiro Sakaguchi, Ryota Nakaoke, Csongor S. Abraham, Hideaki Takahata, Juraj Kopacek, Kazuto Shigematsu, Shigeru Katamine and Masami Niwa :
PrP fragment 106-126 is toxic to cerebral endothelial cells expressing PrPC.,
NeuroReport, Vol.11, No.17, 3931-3936, 2000.

(出版サイトへのリンク): ● Publication site (DOI): 10.1097/00001756-200011270-00064
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1097/00001756-200011270-00064

(DOI: 10.1097/00001756-200011270-00064) Noriyuki Nishida, Patrick Tremblay, Tetsuo Sugimoto, Kazuto Shigematsu, Susumu Shirabe, Chris Petromilli, Susanne Pilkuhn Erpel, Ryota Nakaoke, Ryuichiro Atarashi, Takeshi Houtani, Marilyn Torchia, Suehiro Sakaguchi, Stephen J. DeArmond, Stanley B. Prusiner and Shigeru Katamine :
A mouse prion protein (PrP) transgene rescues Purkinje cell degeneration and demyelination in mice deficient for PrP.,
Laboratory Investigation, Vol.79, No.6, 689-697, 1999. Shigeru Katamine, Noriuki Nishida, Tetsuo Sugimoto, Tetsuo Noda, Suehiro Sakaguchi, Kazuto Shigematsu, Yasufumi Kataoka, Akira Nakatani, Sumitaka Hasegawa, Ryozo Moriuchi and Tsutomu Miyamoto :
Impaired motor coordination in mice lacking prion protein.,
Cellular and Molecular Neurobiology, Vol.18, No.6, 731-742, 1998.

(要約): 1. Prion protein (PrPC) is a host-encoded glycoprotein constitutively expressed on the neuronal cell surface. Accumulation of its protease-resistant isoform is closely related to pathologic changes and prion propagation in the brain tissue of a series of prion diseases. However, the physiological role of PrPC remains to be elucidated. 2. After long-term observation, we noted impaired motor coordination and loss of cerebellar Purkinje cells in the aged mice homozygous for a disrupted PrP gene, a finding which strongly suggests that PrPC plays a role in the long-term survival of Purkinje cells. 3. We also describe the resistance of the PrP null mice to the prion, indicating the requirement of PrPC for both the development of prion diseases and the prion propagation.
(キーワード): Animals / Brain / Cerebellum / Humans / Mice / Mice, Knockout / Motor Activity / Movement Disorders / PrPC Proteins / Prion Diseases / Prions / Purkinje Cells
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 9876879; ● Search Scopus @ Elsevier (PMID): 9876879

(PubMed: 9876879) Suehiro Sakaguchi, Shigeru Katamine, Noriyuki Nishida, Ryozo Moriuchi, Kazuto Shigematsu, Tetsuo Sugimoto, Akira Nakatani, Yasufumi Kataoka, Takeshi Houtani, Susumu Shirabe, Hitoshi Okada, Sumitaka Hasegawa, Tsutomu Miyamoto and Tetsuo Noda :
Loss of cerebellar Purkinje cells in aged mice homozygous for a disrupted PrP gene.,
Nature, Vol.380, No.6574, 528-531, 1996.

(要約): Prion protein (PrP) is a glycoprotein constitutively expressed on the neuronal cell surface. A protease-resistant isoform of prion protein is implicated in the pathogenesis of a series of transmissible spongiform encephalopathies. We have developed a line of mice homozygous for a disrupted PrP gene in which the whole PrP-coding sequence is replaced by a drug-resistant gene. In keeping with previous results, we find that homozygous loss of the PrP gene has no deleterious effect on the development of these mice and renders them resistant to prion. The PrP-null mice grew normally after birth, but at about 70 weeks of age all began to show progressive symptoms of ataxia. Impaired motor coordination in these ataxic mice was evident in a rotorod test. Pathological examination revealed an extensive loss of Purkinje cells in the vast majority of cerebellar folia, suggesting that PrP plays a role in the long-term survival of Purkinje neurons.
(キーワード): Animals / Ataxia / Brain / Cell Death / Drug Resistance / Glutamate Decarboxylase / Heterozygote / Homozygote / Mice / Mutagenesis / Prion Diseases / Prions / Purkinje Cells
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/380528a0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8606772; ● Search Scopus @ Elsevier (PMID): 8606772; ● Search Scopus @ Elsevier (DOI): 10.1038/380528a0

(DOI: 10.1038/380528a0, PubMed: 8606772) Suehiro Sakaguchi, Shigeru Katamine, Kazuto Shigematsu, Akira Nakatani, Ryozo Moriuchi, Noriyuki Nishida, Kenji Kurokawa, Ryota Nakaoke, Hiroshi Sato, Kouichi Jishage, Junko Kuno, Tetsuo Noda and Tsutomu Miyamoto :
Accumulation of proteinase K-resistant prion protein (PrP) is restricted by the expression level of normal PrP in mice inoculated with a mouse-adapted strain of the Creutzfeldt-Jakob disease agent.,
Journal of Virology, Vol.69, No.12, 7586-7592, 1995. Tsutomu Miyamoto, Suehiro Sakaguchi, Shigeru Katamine and Ryozo Moriuchi :
The infectivity is dissociated from PrP accumulation in salivary gland of Creutzfeldt-Jakob disease agent-inoculated mice.,
Annals of the New York Academy of Sciences, Vol.724, 310-313, 1994. Suehiro Sakaguchi, Shigeru Katamine, Kouichi Yamanouchi, Masao Kishikawa, Ryozo Moriuchi, Norio Yasukawa, Takashi Doi and Tsutomu Miyamoto :
Kinetics of infectivity are dissociated from PrP accumulation in salivary glands of Creutzfeldt-Jakob disease agent-inoculated mice.,
The Journal of General Virology, Vol.74, No.Pt10, 2117-2123, 1993.

(要約): The protease-resistant isoform of prion protein (PrP) has been implicated in the pathogenesis and transmission of Creutzfeldt-Jakob disease (CJD), scrapie and other related diseases, but the relationship between the infectious agent and PrP awaits elucidation. In the present study, we have examined levels of infectivity together with accumulation of the protease-resistant form of PrP (PrPCJD) in various tissues of CJD agent-inoculated mice. Accumulation of PrPCJD occurred only in tissues, including brain, salivary gland and spleen, in which infectivity was readily detectable throughout the course of the experiment. The brain showed the highest levels of both infectivity and PrPCJD accumulation, with well correlated kinetics. On the other hand, the high titres of infectivity detected in salivary gland and spleen early after inoculation of the agent were obviously distinguishable from PrPCJD. Furthermore, in the salivary gland, the kinetics of infectivity and the accumulation of PrPCJD reversed; infectivity declined as PrPCJD accumulated in the tissue. Our findings indicate that PrPCJD accumulation is associated with replication of the agent; however, PrPCJD is unlikely to be the agent itself.
(キーワード): Animals / Brain / Creutzfeldt-Jakob Syndrome / Kidney / Kinetics / Liver / Male / Mice / Prions / Salivary Glands / Spleen
(出版サイトへのリンク): ● Publication site (DOI): 10.1099/0022-1317-74-10-2117
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 8409936; ● Search Scopus @ Elsevier (PMID): 8409936; ● Search Scopus @ Elsevier (DOI): 10.1099/0022-1317-74-10-2117

(DOI: 10.1099/0022-1317-74-10-2117, PubMed: 8409936)

MISC

研究者総覧に該当データはありませんでした。

総説・解説

千田淳司, 木戸博, 坂口末廣 :
宿主因子を標的にした新たなインフルエンザ治療の試み,
BIO Clinica, Vol.256, No.33, 52-55, 2018年2月. 内山圭司, 坂口末廣 :
プリオン病における神経変性のメカニズム,
Clinical Neuroscience, Vol.31, No.9, 1022-1024, 2013年9月. 坂口末廣, 森剛志 :
プリオン病,
Clinical Neuroscience, Vol.28, No.8, 906-908, 2010年8月. 坂口末廣 :
プリオン蛋白異常化と伝達・進行のメカニズム,
プリオン病と遅発性ウイルス感染症, 37-43, 2010年7月. 坂口末廣 :
プリオン病予防ワクチンの開発の試み,
バイオ医薬の開発技術とシリーズ, 373-384, 2009年3月.

(キーワード): 山本重夫監修

坂口末廣 :
プリオン病と治療戦略の最近の動向,
BRAIN and NERVE, Vol.61, No.8, 929-938, 2009年. 坂口末廣 :
基礎医学研究の活性化を目指して-若手研究者の育成:今後求められるもの-,
四国医学雑誌, Vol.64, No.1,2, 7-9, 2008年.

(徳島大学機関リポジトリ): ● Metadata: 110215

(徳島大学機関リポジトリ: 110215) 坂口末廣 :
プリオンタンパク質の正常機能とプリオン病における役割,
生化学, Vol.79, No.9, 843-852, 2007年9月.

(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17969324; ● CiNii @ 国立情報学研究所 (CRID): 1520853833087974528; ● Summary page in Scopus @ Elsevier: 2-s2.0-38449116453

(PubMed: 17969324, CiNii: 1520853833087974528, Elsevier: Scopus) 坂口末廣 :
プリオン伝播(プリオン蛋白異常化)のメカニズム,
日本臨牀, Vol.65, No.8, 1391-1395, 2007年8月. 坂口末廣 :
プリオン蛋白質の生理機能,
化学療法の領域, Vol.22, No.1, 56-62, 2006年1月. 坂口末廣 :
プリオン病,
感染症 The infection, Vol.36, No.4, 9-13, 2006年. 坂口末廣 :
プリオン蛋白とプリオン病,
Current Concepts in Infectious Diseases 対談:感染症-今日の話題から, Vol.24, No.3, 3-5, 2005年. 坂口末廣 :
プリオン蛋白類似分子の分子生物学-第2のプリオン蛋白とは-,
医学のあゆみ, Vol.203, No.10, 871-876, 2002年12月. 坂口末廣, 片峰茂 :
正常プリオンタンパクの働き,
ファルマシア, Vol.38, No.7, 640-644, 2002年. 坂口末廣 :
プリオン -プリオン研究の現状と将来の展望—,
ウイルス, Vol.52, No.1, 163-167, 2002年. 坂口末廣 :
クロイツフェルト・ヤコブ病の治療,
臨床検査, Vol.46, No.12, 1527-1532, 2002年. 坂口末廣, 片峰茂 :
ノックアウトマウスとプリオン病,
Clinical Neuroscience, Vol.19, No.8, 36(898)-39(901), 2001年. 坂口末廣, 片峰茂 :
Doppel(Dpl)/PrPLP,
脳の科学, Vol.22, 718-719, 2000年. 坂口末廣, 片峰茂 :
プリオン病とプリオン蛋白,
感染,炎症,免疫, Vol.30, No.4, 2(272)-11(281), 2000年. 坂口末廣, 片峰茂 :
プリオン病の分子病態,
遺伝子医学, Vol.3, 103(321)-107(325), 1999年. 坂口末廣, 片峰茂 :
プリオン病の分子生物学,
ウイルス, Vol.49, No.2, 193-204, 1999年. 坂口末廣, 片峰茂 :
遺伝子操作マウスによるプリオン病の研究,
現代医療, Vol.29, No.12, 87(3133)-91(3137), 1997年. 新竜一朗, 坂口末廣 :
プリオンの分子生物学,
Brain Medical, Vol.9, No.3, 33(249)-38(254), 1997年. 坂口末廣, 宮本勉 :
プリオン病 —概念，疾患，動向—,
小児科診療, Vol.60, No.11, 1771(61)-1776(66), 1997年. 坂口末廣, 片峰茂 :
ノックアウトマウスによるプリオンの解析,
実験医学, Vol.14, No.18, 43(2525)-47(2529), 1996年. 坂口末廣, 宮本勉 :
クロイツフェルト-ヤコブ病の成因とプリオン,
老年期痴呆, Vol.7, No.3, 31(303)-36(308), 1993年.

講演・発表

Keiji Uchiyama and Suehiro Sakaguchi :
Sorting of prion protein and PrPSc accumulation.,
International Prion Congress-Prion 2016, Tokyo, May 2016. Keiji Uchiyama, Tomita Mitsuru and Suehiro Sakaguchi :
Mechanism of sortilin-mediated PrP degradation.,
Asian Pacific Prion Symposium 2015, Ishikawa Ongakudo, Kanazawa, Japan., Sep. 2015. Tomita Mitsuru, Keiji Uchiyama, Usui Takeshi and Suehiro Sakaguchi :
Analysis of roles of N-terminal region of prion protein in the conversion into scrapie form by using prion-knockout cell.,
Asian Pacific Prion Symposium 2015, Ishikawa Ongakudo, Kanazawa, Japan, Sep. 2015. Suehiro Sakaguchi :
Vesicular trafficking in prion disease,
Asian Pacific Prion Symposium 2014, Jul. 2014. Keiji Uchiyama and Suehiro Sakaguchi :
Post-Golgi trafficking of membrane proteins impaired by prion infection.,
Prion, Vol.8, 98, Trieste, Italy, Jun. 2014.

(キーワード): prion / membrane trafficking

Keiji Uchiyama and Suehiro Sakaguchi :
Prions disturb post-Golgi membrane trafficking to the cell surface.,
Proceedings of The 9th International Symposium of the Institute Network, 30, Osaka, Jun. 2014. Suehiro Sakaguchi and Keiji Uchiyama :
Prions impair post-Golgi trafficking of membrane proteins,
Asian Pacific Prion Symposium 2013, Nagasaki, Jul. 2013. Suehiro Sakaguchi :
The role of the N-terminal region of prion protein in prion disease.,
8th IBRO World Congress of Neuroscience International Brain Research Organization, Florence Italy, Jul. 2011. Suehiro Sakaguchi :
Effects of a deletion of the N-terminal part of prion protein on prion replication and pathogenesis in mice,
PRION JAPAN & CANADA Foregoing partnerships. Advancing prion research. U-Port Hotel, Shinagawa, Tokyo, Japan., Nov. 2010. Suehiro Sakaguchi, Miyata Hironori, Yoshitaka Yamaguti, Tsuyoshi Mori and Muramatsu Naomi :
Roles of the N-terminal region of prion protein in prion propagation and pathogenesis,
Asia-Oceania Symposium on Prion Diseases (AOSPD 2010), Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan., Sapporo, Jul. 2010. Suehiro Sakaguchi :
Antagonistic interaction between prion protein and its homologue, PrPLP/Dpl, in neurodegeneration,
AACL-Nagasaki Symposium, ASIAN AGING 2006: The Regional Aging Connection and the Future, Nagasaki, Japan, Jun. 2006. Suehiro Sakaguchi :
Prion protein and prion diseases,
2005 Japan-America Frontiers of Engineering Symposium, San Jose, USA, Nov. 2005. Suehiro Sakaguchi, D Ishibashi and H Yamanaka :
Efficient Induction of Prophylactic antibodies against Prion Disease in Mice,
24th International Congress of Chemotherapy. Symposium 34: Emerging and re-emerging infectious diseases in the Western Pacific, Manila, Philippine, Jun. 2005. S Hashiguchi, S Kitamoto, K Sakamoto, Y Ito, T Nakashima, K Sasaki, JU Gaikwad, K Akasaka, Suehiro Sakaguchi, S Katamine and K Sugimura :
Human antibodies specific to beta-sheet-rich isoform of human prion protein,
PEGS: The Protein Engineering Summit, ``Take Protein Engineering from Concept to Reality'', Cambridge, Massachusetts, USA, May 2005. S Hashiguchi, M Yamamoto, S Kitamoto, T Nakashima, H Yamanaka, D Ishibashi, Suehiro Sakaguchi, S Katamine, Y Ito and K Sugimura :
Prion-conformation-specific human antibodies established from phage display library,
International Symposium, Prion Diseases, Food and Drug safety, Sendai, 2004. H Yamanaka, D Ishibashi, T Tsuji and Suehiro Sakaguchi :
Mucosal immuneogenicity of prion protein fused with heat-labile enterotoxin B subunit,
International Symposium, Prion Diseases, Food and Drug safety, Sendai, Japan, 2004. Suehiro Sakaguchi :
Roles of PrP and PrP-lik proteins (Doppel) in neurodegeneration,
Keystone Symposium, Molecular Aspects of Transmissible Spongiform Encephalopathies (Prion Diseases), Colorado, USA, 2003. K Arima, N Nishida, Suehiro Sakaguchi, S Lehmann and S Katamine :
The strain diversity of the TSE agent in cell-culture models,
International Conference on Transmissible Spongiform Encephalopathies, Edinburgh, UK, 2002. Suehiro Sakaguchi, R Atarashi, K Shigematsu and S Katamine :
Evaluation of pathogenic roles of PrPLP/Dpl in prion diseases,
International Symposium on The New Prion Biology: Basic Science, Diagnosis and Therapy, Venezia, Italy, 2002. Y Kirino, Y Kishimoto, S Kawahara, T Nakaya, Suehiro Sakaguchi and S Katamine :
Age-dependent Disturbance of Delay Eyeblink Conditioning in Prion Protein-Deficient Mice,
Society for Neuroscience, Annual Meeting, Miami Beach, Florida, Oct. 1999. S Katamine, J Kopacek, Suehiro Sakaguchi, R Atarshi, R Nakaoke, A Li, M Niwa and K Shigematsu :
Upregulation of the genes encoding lysosomal hydrolases and a perforin-like protein in microglia colocalyzed with vacuolar neurodegeneration in the brain of mice affected with an experimental prion disease,
Characterization and Diagnosis of Prion Diseases in Animal and Man, Tueingen, Germany, 1999. N Nishida, Suehiro Sakaguchi, R Nakaoke, R Atarashi, A Nakatani, K Shigematsu, S Shirabe, T Sugimoto, P Tremblay, SB Prusiner and S Katamine :
Purkinje cell loss in the PrP-gene knockout mouse is rescued by a wild-type mouse PrP-transgene,
Conferences Philippe Laudat INSERM Molecular Mechanisms of Infection and Pathology in Prion Diseases, Domaine Aix-Marlioz, Aix-les-Bains, France, Oct. 1997. S Katmine, Suehiro Sakaguchi, N Nishida, R Moriuchi, K Shigematsu, A Nakatani, Y Kataoka, T Sugimoto, T Noda and T Miyamoto :
Loss of cerebellar Purkinje neurons in aged mice homozygous for disrupted PrP gene,
International Symposium: Prion diseases, Epidemiology, Biochemistry, and Molecular Biology, Goettingen, Germany, Nov. 1995. S Katamine, Suehiro Sakaguchi, N Nishida, R Moriuchi, K Shigematsu, A Nakatani, Y Kataoka, T Sugimoto, T Noda and T Miyamoto :
Impaired motor coordination and loss of cerebellar Purkinje cells in aged mice homozygous for disrupted PrP gene,
Satellite Symposium of the 15th International Society of Neurochemistry and 7th International Symposium of the Biochemistry and Biophysics on Diagnosis and Treatment of Stroke, Neurotrauma, and Other Neurological Diseases, Kurashiki, Japan, 1995. 千田淳司, 原英二, 清水真祐子, 常山幸一, 坂口末廣 :
Anti-prion antibody treatment attenuates liver inflammation and fibrosis in experimental non-alcoholic steatohepatitis mouse modelAnti-prion antibody treatment attenuates liver inflammation and fibrosis in experimental non-alcoholic steatohepatitis mouse model,
原英之, 千田淳司, 坂口末廣 :
ネクロトーシスをトリガーとした異常型プリオン蛋白質産生の分子機構,
第45回日本分子生物学会年会, 2022年11月. 原英之, 千田淳司, 坂口末廣 :
インフルエンザウイルス感染は神経細胞において感染性プリオンの産生を引き起こす,
第44回日本分子生物学会年会, 2021年12月. 千田淳司, 原英之, 坂口末廣 :
プリオン蛋白質はインフルエンザ A ウイルス感染による重症化を軽減する,
第42回日本分子生物学会年会, 2019年12月. 原英之, 千田淳司, 坂口末廣 :
ウイルス感染を用いたプリオン病発症機構の解明,
第42回日本分子生物学会年会, 2019年12月. 原英之, 千田淳司, 坂口末廣 :
インフルエンザウイルス感染は神経細胞において異常型プリオン産生のトリガーとなる,
第41回日本分子生物学会年会, 2018年11月. 千田淳司, 原英之, 坂口末廣 :
プリオン蛋白質はインフルエンザAウイルス感染に防御的に機能する,
第66回日本ウイルス学会学術集会, 2018年10月. 千田淳司, 原英之, 坂口末廣 :
Prion protein protects mice from lethal infection with Influenza A virues,
2017年度生命科学系学会合同年次大会 (ConBio2017) 第 40回日本分子生物学会年会/第90回日本生化学会大会, 2017年12月. 原英之, 千田淳司, 坂口末廣 :
蛋白質凝集体「プリオン」による抗インフルエンザウイルス活性機構,
2017年度生命科学系学会合同年次大会 (ConBio2017) 第 40回日本分子生物学会年会/第90回日本生化学会大会, 2017年12月. 内山圭司, 坂口末廣 :
Identification and investigation of a novel anti-prion compound.,
第65回日本ウイルス学会学術集会, 2017年10月. 内山圭司, 藤稿智宏, 坂口末廣 :
High susceptibility of Sortilin-deficient cells to prion infection.,
第65回日本ウイルス学会学術集会, 2017年10月. 坂口末廣, 内山圭司 :
Prion propagation through sortilin degradation.,
第60回日本神経化学会大会, 2017年9月. 内山圭司, 坂口末廣 :
プリオン病における異常プリオンの蓄積メカニズム,
第39回日本分子生物学会, 2P-0593, 2016年12月. 原英之, 千田淳司, 坂口末廣 :
蛋白質凝集体「プリオン」による抗インフルエンザウイルス活性機構の解明,
第39回日本分子生物学会年会, 2016年12月. 坂口末廣 :
プリオンの神経毒性及び複製メカニズム．,
徳島大学大学院医歯薬学研究部 2015年度第7回脳科学クラスター・ミニリトリート, 2016年2月. 原英之, 千田淳司, 坂口末廣 :
蛋白質凝集体「プリオン」による抗インフルエンザ活性の発見,
第38回日本分子生物学会年会第88回日本生化学会大会合同大会, 2015年12月. 内山圭司, 富田満, 坂口末廣 :
プリオン感染により過剰な異常プリオンが蓄積する分子メカニズム,
第38回に本分子生物学会年会第88回日本生化学会大会合同大会, 2015年12月. 内山圭司, 富田満, 坂口末廣 :
Inhibition of Sortilin-mediated PrP degradation by prion infection causes excessive accumulation of abnormal prion protein.,
第63回日本ウイルス学会学術集会, 2015年11月. Keiji Uchiyama, 富田満 and Suehiro Sakaguchi :
Novel molecular mechanism for accumulation of abnormal prion protein - Inhibition of Sortilin-mediated PrP degradation.,
第15回蛋白質科学会年会ワークショップ「New development of prion biology」, Jun. 2015.

(キーワード): prion / Sortilin / degradation

坂口末廣, 内山圭司 :
プリオンによるポストゴルジ膜輸送障害,
第36回生体膜と薬物の相互作用シンポジウム「生体膜における蛋白質の機能制御システムと疾患」, 2014年11月. 富田満, 内山圭司, 臼井健, 坂口末廣 :
プリオンノックアウト細胞を用いたプリオンタンパク質N末端領域の異常プリオン形成における役割の解明,
第62回日本ウイルス学会学術集会, 2014年11月. 内山圭司, 富田満, 臼井健, 坂口末廣 :
新規プリオン結合因子Sortilinのプリオン感染における役割,
第62回日本ウイルス学会学術集会, 2014年11月. 清水七海, 古賀雄一, 作道章一, 原英之, 坂口末廣, 金谷茂則 :
超好熱菌由来プロテアーゼによるプリオンタンパク質分解の評価,
第87回日本生化学会, 2014年10月. 内山圭司, 坂口末廣 :
プリオン感染と小胞輸送障害,
第87回日本生化学会シンポジウム認知症克服に向けて:プリオン病をもっと知る., 2014年10月. 坂口末廣 :
プリオン病のイントロダクション,
第87回日本生化学会シンポジウム認知症克服に向けて:プリオン病をもっと知る, 2014年10月. 古賀雄一, 清水七海, 作道章一, 原英之, 坂口末廣, 金谷茂則 :
超好熱菌由来プロテアーゼによるプリオンタンパク質分解の評価,
第66回日本生物工学会大会, 2014年9月. 内山圭司, 坂口末廣 :
プリオン感染によるSortilin発現低下が異常プリオン蓄積を引き起こす,
第29回中国四国ウイルス研究会プログラム・抄録集, 26, 2014年6月.

(キーワード): プリオン / 異常プリオン

内山圭司, 坂口末廣 :
プリオン感染によるポストゴルジ小胞輸送障害,
第66回日本細胞生物学会要旨集, 149, 2014年6月.

(キーワード): プリオン / 小胞輸送

村松直美, 坂口末廣 :
プロテアソーム阻害はプリオン感染細胞においてG2期停止を引き起こしアポトーシスを増悪させる,
第61回日本ウイルス学会学術集会, 2013年11月. 内山圭司, 坂口末廣 :
プリオン感染はポストゴルジ小胞輸送を抑制する,
第61回日本ウイルス学会学術集会, 2013年11月. 村松直美, 坂口末廣 :
Enhanced apoptosis and G2 arrest in prion-infected cells treated with proteasome inhibitors,
日本生化学会, 2013年9月. 内山圭司, 坂口末廣 :
プリオン感染はポストゴルジ小胞輸送障害を引き起こす,
日本生化学会, 2013年9月. 坂口末廣, 内山圭司 :
Inhibition of Post-Golgi Membrane Traffic in Prion-Infected Cells,
第35回分子生物学会年会(ポスター発表), 2012年12月. 坂口末廣, 内山圭司 :
Inhibition of Post-Golgi Membrane Traffic in Prion-Infected Cells,
第35回分子生物学会年会ワークショップ「Membrane Traccic and Diseases」(口頭発表), 2012年12月. Ishibashi Daisuke, Yamanaka Hitoki, Yamaguchi Naohiro, 山口仁孝, Nishida Noriyuki, 坂口末廣 :
細菌由来の蛋白succinylarginine dihydrolaseの免疫によるプリオン病の予防効果,
第40回日本免疫学会, 2011年11月. 森剛志, 村松直美, 犬伏祥子, 山口仁孝, 矢野雅司, 藤田浩司, 坂口末廣 :
プリオン蛋白過剰発現誘導性細胞死の分子機構,
第33回日本分子生物学会年会,第83回日本生化学会大会合同大会, 2010年12月. 坂口末廣, 宮田博規, 山口仁孝, 村松直美, 森剛志 :
異なるプリオン株の産生メカニズム,
第33回日本分子生物学会年会,第83回日本生化学会大会合同大会, 2010年12月. 森剛志, 村松直美, 犬伏祥子, 山口仁孝, 矢野雅司, 藤田浩司, 坂口末廣 :
プリオン蛋白過剰発現誘導性細胞死の分子機構,
第33回日本分子生物学会年会,第83回日本生化学会大会合同大会プリオン蛋白過剰発現誘導性細胞死の分子機構神戸ポートアイランド, 2010年12月. 村松直美, 森剛志, 山口仁孝, 藤田浩司, 坂口末廣 :
A prion protein with familial mutation, PrP-Y145Stop, induces cell death through G2 cell cycle arrest,
第33回日本分子生物学会年会,第83回日本生化学会大会合同大会神戸ポートアイランド, 2010年12月. 清水一史, 佐々木裕, 芝田敏克, 田中寅彦, 黒田和道, 坂口末廣, 片峰茂, 山本樹生 :
インフルエンザウイルス感染による選択的スプライシングの誘導,
第58回日本ウイルス学会あわぎんホール(徳島県郷土文化会館), 2010年11月. 村松直美, 森剛志, 山口仁孝, 坂口末廣 :
培養細胞を用いたプリオンの細胞死誘導のメカニズム,
第58回日本ウイルス学会あわぎんホール(徳島県郷土文化会館), 2010年11月. 森剛志, 村松直美, 犬伏祥子, 山口仁孝, 坂口末廣 :
プリオン蛋白の過剰発現は細胞死を誘導する,
第58回日本ウイルス学会あわぎんホール(徳島県郷土文化会館), 2010年11月. 山口仁孝, 村松直美, 森剛志, 坂口末廣 :
プリオン蛋白構造変換におけるアミノ酸91-104領域の役割,
第58回日本ウイルス学会, 2010年11月. 坂口末廣, 宮田博規, 山口仁孝, 村松直美, 森剛志 :
異なるプリオン株に異なる感受性を示すプリオン蛋白:プリオン株産生メカニズムについての考察,
第58回日本ウイルス学会あわぎんホール(徳島県郷土文化会館), 2010年11月. 坂口末廣 :
感染性タンパク質``プリオン'',
第一回全国共同利用・共同研究「酵素学研究拠点」シンポジウム—酵素学から始まる新たな創薬研究—, 2010年9月.

研究会・報告書

千田淳司, 原英之, 坂口末廣 :
肺で発現する正常プリオン蛋白質の機能解析,
第32回中国四国ウイルス研究会, 2017年6月. 坂口末廣 :
プリオン病の分子生物学,
徳島文理大学薬学部病態分子薬理学研究室大学院特別講義, 2016年12月. 坂口末廣 :
次世代抗インフルエンザ薬の宿主ターゲット分子の発見とその治療効果,
四国地区五大学新技術説明会, 2015年11月. 坂口末廣 :
プリオン病におけるポストゴルジ小胞輸送障害,
革新的医療研究開発で挑む神経変性疾患—プリオン病治験耐性の確立に向けてー, 2015年2月. 坂口末廣 :
蛋白質感染粒子「プリオン」と細胞内小胞輸送,
第8回共同利用・共同研究「酵素学研究拠点」シンポジウム—タンパク質代謝・分解系の酵素学—, 2015年2月. 内山圭司, 臼井健, 矢野雅司, 坂口末廣 :
プリオン感染はポストゴルジ小胞輸送を抑制する,
第28回中国四国ウイルス研究会, 2013年6月. 内山圭司, 臼井健, 矢野雅司, 坂口末廣 :
プリオン感染によるポストゴルジ小胞輸送抑制,
第54回日本生化学会中国・四国支部例会, 2013年6月. 内山圭司, 坂口末廣 :
プリオン感染は神経細胞におけるインスリンシグナル異常を引き起こす,
第27回中国四国ウイルス研究会抄録集, 2012年6月.

(キーワード): プリオン

坂口末廣 :
プリオン病の基礎研究:プリオン蛋白の病態機序,
第11回徳島神経難病セミナー, 2012年1月. 内山圭司, 坂口末廣 :
プリオン感染細胞における細胞内小胞輸送の抑制,
第26回中国四国ウイルス研究会抄録集, 2011年6月.

(キーワード): プリオン / ゴルジ体

坂口末廣, 宮田博規, 山口仁孝, 村松直美, 森剛志, 内山圭司, 犬伏祥子 :
異なるプリオン株の産生メカニズムについて,
第26回中国四国ウイルス研究会徳島大学青藍会館徳島, 2011年6月. Suehiro Sakaguchi :
Roles of a prion protein family in neurodegeneration,
Enzyme Research Forum 2011 in Nantong University. Nantong University. China., Mar. 2011. 森剛志, 村松直美, 山口仁孝, 犬伏祥子, 矢野雅司, 藤田浩司, 坂口末廣 :
プリオン蛋白過剰発現により誘導される細胞死の機序,
第26回中国四国ウイルス研究会岡山大学創立50周年記念館岡山, 2010年6月.

特許: 坂口末廣, 千田淳司 : 非アルコール性脂肪肝炎を治療または予防するための医薬組成物, (2022年1月), (2023年8月), 特許第2022-008773号 (2022年1月). 坂口末廣, 千田淳司 : 抗プリオン蛋白質抗体とその用途, (2015年4月), 特許第2015-077742号 (2015年4月).
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: プリオン病の予防・治療法開発を目指したプリオン以外の病原体の究明と病態解明研究 (研究課題/領域番号: 23K27489 )
プリオン病の神経細胞障害のメカニズム解明とその障害を改善する化合物の探索 (研究課題/領域番号: 19H03548 )
プリオン蛋白質は耐性ウイルスを誘導しない夢のインフルエンザ薬のターゲットである (研究課題/領域番号: 17K19661 )
プリオン以外のプリオン病の病原体の同定とその病原性メカニズムの解明 (研究課題/領域番号: 17H05701 )
プリオンの増殖と病原性獲得に重要なプリオンの細胞内移動に関与する分子の同定 (研究課題/領域番号: 15H01560 )
我々の最新の発見は薬剤耐性ウイルスを誘導しない夢の抗インフルエンザ薬を可能にする (研究課題/領域番号: 15K15380 )
新規プリオン結合因子ソーティリンによるプリオン病治療基盤技術の開発 (研究課題/領域番号: 26460557 )
プリオン病の新規病態として発見した細胞膜蛋白質の小胞輸送障害のメカニズム解明 (研究課題/領域番号: 26293212 )
プリオン感染に必須なプリオン蛋白質以外の分子の同定 (研究課題/領域番号: 25670422 )
BSE(狂牛病)超高感度診断法の開発 (研究課題/領域番号: 20580354 )
神経細胞におけるプリオン蛋白の分子シグナルの解明 (研究課題/領域番号: 16590387 )
神経細胞ストレス回避機構の機能プロテオミクスープリオンと新規神経保護蛋白質の役割 (研究課題/領域番号: 15390028 )
プリオン病における神経細胞変性死の分子機構解明 (研究課題/領域番号: 15016087 )
プリオン受容体を構成する分子の同定 (研究課題/領域番号: 14570267 )
PrP pre-mRNA 3'プロセッシング異常と神経細胞死の分子機構解明 (研究課題/領域番号: 14035244 )
プリオン病における神経細胞変性死の分子機構解明 (研究課題/領域番号: 14017076 )
プリオン病における神経細胞変性死の分子機構解明 (研究課題/領域番号: 13210112 )
プリオン病における神経細胞変性死の分子機構解明 (研究課題/領域番号: 12210119 )
プリオン蛋白欠損マウスで認められる加齢に伴う神経細胞変性死の分子機構解明 (研究課題/領域番号: 12050232 )
プリオン蛋白欠損マウスにおける小脳プルキンエ細胞死と脱髄機序の解明 (研究課題/領域番号: 11694292 )
プリオン蛋白(PrP)結合ペプチドの同定とそのプリオン病治療への応用 (研究課題/領域番号: 10878140 )
プリオン病におけるプリオン蛋白変換の分子機構解明と脳組織内変換の場の特定 (研究課題/領域番号: 09470154 )
プリオン遺伝子欠損マウスを用いたクロイツフェルト・ヤコブ病病原因子の本態解明 (研究課題/領域番号: 09470086 )
プリオン蛋白欠損マウスにおける神経細胞死の分子機構 (研究課題/領域番号: 09044321 )
クロイツフェルト・ヤコブ病の病原因子はプリオンでない可能性がある (研究課題/領域番号: 08877056 )
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2024年11月22日更新

専門分野・研究分野: 医学 (Medicine)
所属学会・所属協会: アジア太平洋プリオン研究会
中国四国ウイルス研究会
委員歴・役員歴: アジア太平洋プリオン研究会 (理事長 [2013年8月〜2015年7月])
中国四国ウイルス研究会 (幹事 [2006年6月〜2016年3月])
受賞: 2013年7月, best presentation prize (Asian Pacific Prion Symposium 2013)
活動: 研究者総覧に該当データはありませんでした。

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Jグローバル最終確認日: 2024/11/16 01:21
氏名（漢字）: 坂口末廣
氏名（フリガナ）: サカグチスエヒロ
氏名（英字）: Sakaguchi Suehiro
所属機関: 徳島大学教授

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researchmap最終確認日: 2024/11/17 01:33
氏名（漢字）: 坂口末廣
氏名（フリガナ）: サカグチスエヒロ
氏名（英字）: Sakaguchi Suehiro
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登録日時: 2002/10/18 00:00
更新日時: 2021/12/10 11:34
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所属ID: 0344000000
所属: 徳島大学
部署: 先端酵素学研究所
職名: 教授
学位: 博士（医学）
学位授与機関: 長崎大学
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経歴
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2024年11月16日更新

研究者番号: 60274635

所属（現在）: 2024/4/1 : 徳島大学, 先端酵素学研究所, 教授

所属（過去の研究課題 情報に基づく）*注記: 2023/4/1 – 2024/4/1 : 徳島大学, 先端酵素学研究所, 教授
2020/4/1 – 2021/4/1 : 徳島大学, 先端酵素学研究所, 教授
2016/4/1 – 2019/4/1 : 徳島大学, 先端酵素学研究所(次世代), 教授
2016/4/1 : 徳島大学, 先端酵素学研究所, 教授
2013/4/1 – 2015/4/1 : 徳島大学, 疾患酵素学研究センター, 教授
2008/4/1 – 2010/4/1 : 徳島大学, 疾患酵素学研究センター, 教授
2004/4/1 – 2005/4/1 : 長崎大学, 大学院・医歯薬学総合研究科, 助教授
2002/4/1 – 2004/4/1 : 長崎大学, 大学院・医歯薬学総合研究科, 講師
2000/4/1 – 2001/4/1 : 長崎大学, 大学院・医学研究科, 講師
2000/4/1 : 長崎大学, 医学研究科, 講師
1999/4/1 : 長崎大学, 医学部, 講師
1997/4/1 – 1998/4/1 : 長崎大学, 医学部, 助手

審査区分/研究分野

研究代表者

医学 / 病理 / ウイルス学
生物系 / 医歯薬学 / 内科系臨床医学 / 神経内科学
生物系 / 医歯薬学 / 基礎医学 / ウイルス学
生物系 / 医歯薬学 / 内科系臨床医学 / 感染症内科学
内科学一般およびその関連分野
生物系
小区分51030:病態神経科学関連

研究代表者以外

複合領域 / 神経科学(含脳科学) / 神経化学・神経薬理学
生物系 / 農学 / 畜産学・獣医学 / 臨床獣医学
複合領域 / 神経科学(含脳科学) / 神経科学一般
医学 / 内科 / 神経内科学
医学 / 病理 / ウイルス学
生物系 / 医歯薬学 / 薬学 / 生物系薬学
生物系 / 医歯薬学 / 基礎医学 / ウイルス学

キーワード

研究代表者

プリオン / プリオン病 / プリオン蛋白 / プリオン蛋白欠損マウス / クロイツフェルト・ヤコブ病 / 遺伝子欠損マウス / 伝播性海綿状脳症 / プリオン蛋白(PrP) / ノックアウトマウス / プルキンエ細胞 / 神経変性 / プリオン蛋白類似蛋白(PrPLP) / 遺伝子導入マウス / プリオン蛋白類似蛋白 / 神経細胞変性 / ファージディスプレイ法 / ペプチド療法 / 受容体 / トランスジェニックマウス / intergenic splicing / pre-mRNA / 3'プロセッシング / siRNA / 神経芽細胞腫 / ソーチリン / プリオンタンパク質 / 感受性 / 構造変換 / 狂牛病 / 蛋白質輸送 / 蛋白質分解 / 神経変性疾患 / プリオン蛋白質 / アトラクチン / インスリン受容体 / ポストゴルジ小胞輸送 / 小胞輸送 / 細胞膜蛋白質 / Tgマウス / ポストゴルジ輸送 / リポソーム / prion / knockout mouse / liposome / 結合分子 / アルカリフォスファターゼ / 結合蛋白 / リコンビナント蛋白 / two-hybrid法 / タグ / クローニング / prion protein / binding protein / receptor / alkali phosphatase / シグナル / yeast-two hybrid / 細胞死 / 凝集体 / 蛋白分解 / yeast-two hyb / yeast two-hybrid / binding molecule / prion disease / cell death / aggregate / インフルエンザ / インフルエンザウイルス / 活性酸素種 / 銅イオン / 銅/亜鉛依存性スーパーオキシドジスムターゼ / アポトーシス / 酸化ストレス / 抗体 / リード化合物 / 細胞内小胞輸送 / スーパーオキサイドジスムターゼ / Src / Srcファミリーキナーゼ / マクロファージ / A型インフルエンザウイルス / 銅 / スーパーオキシドジスムターゼ / 異常プリオン蛋白質 / 病原体 / インターロイキン1β / エタノールアミン / 神経細胞死

研究代表者以外

プリオン病 / プリオン蛋白 / ペプチド / ファージディスプレイ / 獣医学 / 蛋白質 / 脳神経疾患 / ターゲティングマウス / トランスジェニックマウス / 神経変性 / プルキンエ細胞 / 軸索 / プリオン / 遺伝子改変動物 / 神経細胞死 / アストロサイト / 脱髄 / PRION PROTEIN / TARGETED MYTAGENESIS / PURKINJE CELLS / NEURODEGENERATION / DEMYELINATION / 蛋白蛋白相互作用 / 発症遅延 / ノックアウトマウス / 発症予防 / ペプチドライブラリ / 遺伝子改変マウス / ニューロン / グリア / PRION DISEASES / PEPTIDES / THERAPEUTICS / PROTEIN-PROTEIN INTERACTION / RNAスプライシング / 遺伝子ノックアウトマウス / 生理機能 / ファージデイスプレイ / Prion diseases / Prion protein / Neurodegeneration / RNA splicing / Gene-knockout mice / ストレス / non-classical release / S100A13 / FGF-1 / 無血清 / Ca^<2+> / STI / NDI / ネクローシス / 分泌 / 核移行 / 低酸素 / S100 / Stress / non-vesicular release / serum starvation / 異常プリオン / Sortilin / ソーティング / レトロマー / VPS35 / ソーティリン / プリオン感染 / レトロマー複合体 / タンパク質分解 / プリオン分解 / タパク質分解 / プリオン高感受性 / ンソーティリン欠損マウス / ソーティリン欠損細胞

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坂口 末廣

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坂口末廣