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後藤優樹

徳島大学

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2024年11月22日更新

職名: 准教授
電話: 088-656-7515
電子メール: goto@tokushima-u.ac.jp
学歴: 2008/3: 徳島大学大学院工学研究科博士後期課程修了
学位: 博士(工学) (徳島大学) (2008年3月)
職歴・経歴: 2008/4: 徳島大学助教, 大学院ソシオテクノサイエンス研究部 (-2009.3.)
2010/7: 徳島大学助教, 大学院ヘルスバイオサイエンス研究部 (-2011.9.)
2011/10: 徳島大学助教, 大学院ソシオテクノサイエンス研究部 (-2016.3.)
2016/4: 徳島大学助教, 大学院生物資源産業学研究部 (-2017.3.)
2017/4: 徳島大学助教, 大学院社会産業理工学研究部 (-2021.9.)
2021/10: 徳島大学准教授, 大学院社会産業理工学研究部

専門分野・研究分野: 生物物理化学 (Biophysical Chemistry)

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2024年11月22日更新

専門分野・研究分野: 生物物理化学 (Biophysical Chemistry)
担当経験のある授業科目: 卒業研究 (学部)
応用生命創成実習 (学部)
応用生命学概論 (学部)
応用生命実習 (学部)
応用生命演習Ⅰ (学部)
応用生命科学特別演習 (大学院)
応用生命科学特別研究 (大学院)
物理化学実習 (学部)
生物化学工学特論 (大学院)
生物物理化学 (学部)
生物資源学研究 (大学院)
生物資源産業学A (学部)
生物資源産業学実習 (学部)
発酵学実習 (学部)
英語論文講読 (学部)
英語論文講読Ⅰ (学部)
英語論文講読Ⅱ (学部)
指導経験: 9人 (学士), 20人 (修士)

研究者総覧で最新データを確認する

2024年11月22日更新

専門分野・研究分野: 生物物理化学 (Biophysical Chemistry)

研究テーマ: 脂質ナノ粒子(LNP)の高圧物性と産業的応用 (脂質二重膜, 界面活性剤, 高圧, 相転移, 蛍光イメージング, 放射光, 麻酔, 膜融合)

著書

後藤優樹 :
脂質の安定性(DSC)–水和結晶・非二重膜転移–, 熱量測定・熱分析ハンドブック第3版,(日本熱測定学会編集),
丸善出版, 東京, 2020年8月. Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Encyclopedia of Biocolloid and Biointerface Science, --- Bilayer Imaging of Phosphatidylcholines by High-Pressure Fluorometry ---,
John Wiley & Sons, Inc., Hoboken, Sep. 2016. Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki :
Encyclopedia of Biocolloid and Biointerface Science, --- Phase Separation in Phospholipid Bilayers Induced by Cholesterol ---,
John Wiley & Sons, Inc., Hoboken, Sep. 2016. 松木均, 後藤優樹, 玉井伸岳, 金品昌志 :
高圧バイオサイエンスとバイオテクノロジー, --- 第17章リン脂質二重膜の圧力誘起膜融合:巨大単層ベシクルの球形成長 ---,
三恵社, 名古屋, 2015年11月. 後藤優樹, 楠部真崇, 玉井伸岳, 松木均, 金品昌志 :
高圧力下の生物科学, --- 第4章リン脂質二分子膜ゲル相の多形と安定性に及ぼす圧力効果 ---,
さんえい出版株式会社, 京都, 2006年11月. 多田佳織, 後藤優樹, 楠部真崇, 玉井伸岳, 松木均, 金品昌志 :
高圧力下の生物科学, --- 第3章ジラウロイルホスファチジルコリン二分子膜の圧力誘起相転移 ---,
さんえい出版株式会社, 京都, 2006年11月.

論文

Masaki GOTO, Shuntaro Yoshida, Shigeyuki Habara, Agnieszka Wilk-Kohlbrecher, Joachim Kohlbrecher, Nobutake Tamai and Hitoshi Matsuki :
A molecular mechanism for how pressure induces interdigitation of phospholipid bilayer membranes,
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1866, No.8, 184385-1-184385-9, 2024.

(要約): The phase transition from the bilayer gel (ripple gel) phase to the nonbilayer gel (interdigitated gel) phase of bilayers of phosphatidylcholines (PCs) with two saturated long-chain fatty acids under high pressure was investigated by pressure-scanning microscopy, fluorometry, and dynamic light scattering (DLS) measurements. Microscopic observations for giant vesicles (GVs) of distearoyl-PC (DSPC) under high pressure showed that spherical GVs transforms into warped and distorted spherical ones instantaneously at the interdigitation. The fluorescence intensities of amphiphilic probe Prodan and hydrophobic probe Laurdan in the dipalmitoyl-PC (DPPC) bilayer steeply decreased and increased, respectively, at the interdigitation, suggesting that the conformational change of the polar head group of DPPC in the bilayer transiently occurred at the interdigitation. Further, the high-pressure DLS measurements revealed that the size of the vesicle particles of DPPC and DSPC transiently increased near the interdigitation pressure, whereas the ethanol-induced interdigitation of the DSPC bilayer under atmospheric pressure produced no such change in the particle size. Taking account of the critical packing parameter of the PC molecule, we could conclude that the pressure-induced interdigitation is attributable to the increase in repulsive interaction between the polar head groups of the PC molecules resulting from the orientational change of the head group from a parallel alignment to a perpendicular one with respect to the bilayer surface with pressure, namely the transient state: it occurs when the repulsive interaction exceeds a threshold value for the balance between the repulsive interaction and the attractive interaction among the hydrophobic acyl chains.
(キーワード): 指組み構造化 / 非二分子膜 / 相転移 (phase transition) / リン脂質 / 圧力 (pressure)
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamem.2024.184385
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2024.184385

(DOI: 10.1016/j.bbamem.2024.184385) Nobutake Tamai, Hitoshi Matsuki and Masaki GOTO :
Phase Imaging of Phosphatidylcholine Bilayer Membranes by Prodan Fluorescence,
Membranes, Vol.12, No.12, 1219-1-1219-17, 2022.

(要約): Prodan (6-propiponyl-2-(N,N-dimethylamino)naphthalene) is well known as a polarity-sensitive fluorescent probe and has a high capability of detecting structural changes occurring within phospholipid bilayer membranes. In this study, we carried out the fluorescence spectroscopic observation of bilayer phase behavior for a series of symmetric saturated diacylphosphatidylcholines (CnPCs) with different acyl-chain length n (n = 12 15 and 19 22) using Prodan as a membrane probe to confirm the availability of Prodan along with the previous results for the CnPC bilayer membranes (n = 16 18). The results were discussed by constructing spectral three-dimensional (3D) imaging plots for visualizing the change in bilayer phase states with temperature or pressure to verify the functionality of this 3D imaging plot. It was found that the Prodan fluorescence technique is applicable to the detection of the changes in the bilayer phase states of all CnPCs with a few exceptions. One of the most crucial exceptions was that Prodan cannot be used for the detection of the bilayer-gel state of the C21PC bilayer membrane. It was also found that it is only to the CnPC bilayer membranes with n = 15 18 that the 3D imaging plot is adequately and accurately applicable as a useful graphical tool for visually detecting the bilayer phase states. This is a disadvantageous feature of this technique brought about by the high sensitivity of Prodan as a membrane probe. Therefore, further detailed studies on the molecular behavior of Prodan will enable us to find a more useful way of utilizing this membrane probe.
(キーワード): diacylphosphatidylcholine / Prodan (6-propiponyl-2-(N,N-dimethylamino)naphthalene) / bilayer membrane / 相転移 (phase transition) / 圧力 (pressure)
(徳島大学機関リポジトリ): ● Metadata: 118067
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/membranes12121219
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.3390/membranes12121219

(徳島大学機関リポジトリ: 118067, DOI: 10.3390/membranes12121219) Toshiki Nakao, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki :
Temperature- and Pressure-Induced Bilayer Phase Transitions of an Amide-Linked Phosphatidylcholine: A Contrasting Effect of Chain Linkage Type,
Bulletin of the Chemical Society of Japan, Vol.95, No.2, 261-270, 2022.

(要約): An unnatural phospholipid with two amide linkages, dipalmitoylamidodeoxyphosphatidylcholine (DPADPC), was synthesized and its bilayer phase transitions were observed by differential scanning calorimetry under atmospheric pressure and light-transmittance measurements under high pressure. The DPADPC bilayer membrane showed different phase transitions depending on the thermal history of the lipid sample under atmospheric pressure. The thermal-history dependent transitions were also observed under high pressure. The temperature pressure phase diagram and thermodynamic quantities of the phase transitions of the DPADPC bilayer membrane were compared with those of bilayer membranes of hydrophobic chain-linkage isomers, ester-liked dipalmitoylphosphatidylcholine (DPPC) and ether-linked dihexadecylphosphatidylcholine (DHPC). The thermodynamic quantities of the main transition for these PC bilayer membranes were similar to each other, whereas the suppression of the interdigitation and great stabilization of the hydrated crystal phase were observed for the DPADPC bilayer membrane. Furthermore, the results of nuclear magnetic resonance measurements indicated that the motility of PC molecules in both gel and liquid crystal phases of the DPADPC bilayer membranes were much lower than those of the DPPC and DHPC bilayer membranes. From the above results, we concluded that the molecular interaction in the PC bilayer membranes increases in the order of ether-, ester- and amide-linkage isomers.
(キーワード): Amide-linked phospholipid / Lipid bilayer membrane / 相転移 (phase transition)
(出版サイトへのリンク): ● Publication site (DOI): 10.1246/bcsj.20210395
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1246/bcsj.20210395

(DOI: 10.1246/bcsj.20210395) Masaki GOTO, Akira Kazama, Kensuke Fukuhara, Honami Sato, Nobutake Tamai, Hiro-O Ito and Hitoshi Matsuki :
Membrane fusion of phospholipid bilayers under high pressure: Spherical and irreversible growth of giant vesicles,
Biophysical Chemistry, Vol.277, 106639-1-106639-11, 2021.

(要約): Membrane fusion of giant vesicles (GVs) for binary bilayers of unsaturated phospholipids, dioleoylphosphatidylethanolamine (DOPE) having an ability to promote membrane fusion and its homolog dioleoylphosphatidylcholine (DOPC) having an ability to form GV, was investigated under atmospheric and high pressure. While DOPC formed GVs in the presence of inorganic salts with a multivalent metal ion under atmospheric pressure, an equimolar mixture of DOPE and DOPC formed GVs both in the absence and the presence of LaCl3. We examined the change in size and shape of the GVs of this binary mixture in the absence and presence of LaCl3 as a function of time under atmospheric and high pressure. The size and shape of the GVs in the absence of LaCl3 under atmospheric and high pressure and those in the presence of LaCl3 under atmospheric pressure hardly changed with time. By contrast, the GV in the presence of LaCl3 under high pressure gradually changed in the size and shape with time on a time scale of several hours. Namely, the GV became larger than the original GV due to accelerated membrane fusion and its shape became more spherical. This pressure-induced membrane fusion was completely irreversible, and the growth rate was correlated with the applied pressure. The reason for the GV growth by applying pressure was considered on the basis of thermodynamic phase diagrams. We concluded that the growth is attributable to a closer packing of lipid molecules in the bilayer resulting from their preference of smaller volumes under high pressure. Furthermore, the molecular mechanism of the pressure-induced membrane fusion was explored by observing the fusion of two GVs with almost the same size. From their morphological changes, we revealed that the fusion is caused by the actions of Laplace and osmotic pressure.
(キーワード): Giant vesicle / 高圧力 (high pressure) / Laplace pressure / Lipid bilayer membrane / Osmotic pressure / Membrane fusion
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bpc.2021.106639
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2021.106639

(DOI: 10.1016/j.bpc.2021.106639) Masaki GOTO, Sawaguchi Hiroshi, Nobutake Tamai and Hitoshi Matsuki :
Subgel-phase formation of membranes of ether-linked phosphatidylcholines,
Chemistry and Physics of Lipids, Vol.239, 105119-1-105119-8, 2021.

(要約): The formation of subgel (so-called hydrated crystal) phase of membranes of ether-linked phospholipids, dialkylphosphatidylcholines containing linear saturated alkyl chain (Cn = 14, 16 and 18), was examined under atmospheric and high pressure. The results of differential scanning calorimetry in 50 wt% aqueous ethylene glycol solution and water showed that these PC membranes undergo the subtransition from the subgel phase to the gel phase at a low temperature with or without the thermal pretreatment of lipid samples called annealing. The subtransition in water could be clearly observed by light-transmittance measurements under high pressure and the transition temperature increased by applying pressure. The temperature-pressure phase diagrams and the thermodynamic quantities of subtransition were obtained from the phase-transition data and compared with those of membranes of ester-linked phospholipids, diacylphosphatidylcholines. The phase diagrams indicated that all gel phases of the ether-linked PC membranes exist as stable phases while parts of the gel phases of the ester-linked PC membranes are metastable. The subtransition temperatures of the ether-linked PC membranes were lower than those of the ester-linked PC membranes by more than 10 °C and the corresponding thermodynamic quantities were extremely small. Further, it was revealed by high-pressure fluorometry that the difference in subgel phase between ether- and ester-linked PC membranes results from their phase structures: the nonbilayer interdigitated structure is maintained after the conversion from the gel phase to the subgel phase in the ether-linked PC membranes whereas the ester-linked PC membranes form bilayer subgel phase with staggered structure.
(キーワード): Ether-linked phospholipid / Interdigitation / 相転移 (phase transition) / Phospholipid membrane / 圧力 (pressure) / Subgel phase
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.chemphyslip.2021.105119
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2021.105119

(DOI: 10.1016/j.chemphyslip.2021.105119) Hitoshi Matsuki, Masaki GOTO, Makiko Motohashi, Aoi Kiguchi, Toshiki Nakao and Nobutake Tamai :
Formation of intermediate gel-liquid crystalline phase on medium-chain phosphatidylcholine bilayers: Phase transitions depending on the bilayer packing,
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1862, No.5, 183197-1-183197-11, 2020.

(要約): The bilayer phase transitions of medium-chain phosphatidylcholines with linear saturated acyl chains (Cn = 12, 13 and 14) were measured by high-pressure light-transmittance measurements and differential scanning calorimetry to investigate the formation of intermediate gel-liquid crystalline phase called Lx phase. The constructed phase diagrams showed that there existed a distinct region of the Lx phase between ripple gel (Pβ') and liquid crystalline (Lα) phases for multilamellar vesicle bilayers of C12PC and C13PC. The Lx phase of the C12PC bilayer was metastable at all pressures and disappeared at a higher pressure. In the C13PC bilayer, the Lx phase was stable and also disappeared at a higher pressure but its region markedly shrunk. By contrast, the Lx phase was not detected for the C14PC bilayer. Effects of other factors such as vesicle size and solvent substitution on the Lx phase of the C13PC bilayer were also examined. A decrease in vesicle size and solvent substitution from water to 50 wt% ethylene glycol solution promoted the Lx-phase formation as opposed to the effects of acyl-chain elongation and pressurization. The fluorescence data of the C13PC bilayer with different vesicle sizes showed that the Lx phase is caused by the difference of local packing in the bilayer. Considering these facts, we concluded that the Lx phase is an intermediate gel-Lα phase that has gel-phase monolayers with negative curvature and Lα-phase monolayers with positive curvature. The formation mechanism of the Lx-phase in stacked bilayers and dispersed vesicles is also explainable by this difference in packing state.
(キーワード): 二分子膜 / ゲル-液晶中間相 / 充填状態 / 相転移 (phase transition) / ホスファチジルコリン / 圧力 (pressure)
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamem.2020.183197
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2020.183197

(DOI: 10.1016/j.bbamem.2020.183197) Masaki GOTO, Hirotsugu Okamoto, Nobutake Tamai, Kazuhiro Fukada and Hitoshi Matsuki :
Salt effect on bilayer phase transitions of dipalmitoylphosphatidylglycerol in saline water under high pressure,
High Pressure Research, Vol.39, No.2, 238-247, 2019.

(要約): The phase transitions of dipalmitoylphosphatidylglycerol (DPPG) bilayer membrane at high NaCl concentrations under high pressure were investigated to construct the temperature pressure phase diagram and determine phase-transition properties. The constructed phase diagrams exhibited the qualitative resemblance to that of the dipalmitoylphosphatidylcholine (DPPC) bilayer membrane: they underwent the gel-phase polymorphism including the pressure-induced bilayer interdigitation. The phase-transition properties of the DPPG bilayer membrane changed in a salt concentration-dependent manner, whereas qualitatively the phase behavior is not affected appreciably by the salt concentration measured. We discussed the salt effect on the DPPG bilayer membrane from the variation in interactions between the polar head group of the PG molecule.
(キーワード): 二分子膜 / ジパルミトイルホスファチジルグリセロール / 指組み構造 / 相転移 / 圧力 / プロダン蛍光
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/08957959.2019.1601189
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85063877150

(DOI: 10.1080/08957959.2019.1601189, Elsevier: Scopus) Nobutake Tamai, Sanae Inazawa, Saori Takeuchi, Masaki GOTO and Hitoshi Matsuki :
Phase Behavior of Binary Bilayer Membrane of Dipalmitoylphosphatidylcholine and Stigmasterol,
Journal of Thermal Analysis and Calorimetry, Vol.135, No.5, 2635-2645, 2019.

(要約): リン脂質ジパルミトイルホスファチジルコリン-スティグマステロール混合二分子膜の熱的相挙動を示差走査熱量測定と蛍光プローブ測定から調査した．本系に対して得られた温度-濃度相図はジパルミトイルホスファチジルコリン-コレステロール混合二分子膜のものと類似していたが，スティグマステロールはコレステロールに比べて，ゲル相では脂質アシル鎖に大きな撹乱効果を示す一方で，鎖融解の抑制効果は弱いことが明らかとなった．
(キーワード): コレステロール (cholesterol) / Incongruent melting / Lipid bilayer / Liquid ordered phase / 相図 (phase diagram) / Stigmasterol
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10973-018-7597-9
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85051429077

(DOI: 10.1007/s10973-018-7597-9, Elsevier: Scopus) Nobutake Tamai, Maiko Uemura, Takuya Izumikawa, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Phase Behavior of Cholesterol-Containing Binary Membrane of an Ether-Linked Phospholipid, Dihexadecylphosphatidylcholine,
Colloid and Polymer Science, Vol.296, No.4, 697-711, 2018.

(要約): エーテル結合型リン脂質ジヘキサデシルホスファチジルコリン-コレステロール混合二分子膜の熱的相挙動を示差走査熱量測定と蛍光プローブ測定から調査した．コレステロール添加は指組み構造ゲル相の形成を抑制した．エステル結合型脂質ジパルミトイルホスファチジルコリン-コレステロール混合二分子膜系の相図との比較から，脂質頭部のカルボニル炭素の有無が二分子膜中における両分子の混和性に大きな影響を与えることが明確となった．
(キーワード): コレステロール (cholesterol) / Dihexadecylphosphatidylcholines / Interdigitation / Liquid ordered phase / 相図 (phase diagram) / Peritectic point
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00396-018-4280-4
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1007/s00396-018-4280-4

(DOI: 10.1007/s00396-018-4280-4) Hitoshi Matsuki, Kentaro Kato, Hirotsugu Okamoto, Shuntaro Yoshida, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Ligand partitioning into lipid bilayer membranes under high pressure: implication of variation in phase-transition temperatures,
Chemistry and Physics of Lipids, Vol.209, 9-18, 2017.

(要約): ジパルミトイルホスファチジルコリン(DPPC)二分子膜の相転移温度への2種類の膜活性リガンド(麻酔薬ハロセン，脂肪酸パルミチン酸)の効果を調べた．DPPC二分子膜の前転移および主転移は，ハロセン添加により降下，パルミチン酸添加により逆に上昇し，加圧は相転移を温度を共に上昇させた．熱力学的解析からハロセンは液晶相，パルミチン酸はラメラゲル相に親和性を有し，ハロセン添加により液晶相が膨張し，パルミチン酸添加によりゲル相が収縮することがわかった．また，ハロセンは高圧力下における指組みゲル相形成を促進し，パルミチン酸は逆に抑制した．指組みゲル相を含む相転移挙動へのリガンド効果についての議論が熱力学観点からなされた．
(キーワード): High pressure / Inhalation anesthetic / Ligand partitioning / Lipid bilayer membrane / Long-chain fatty acid / Phase transition
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.chemphyslip.2017.10.002
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2017.10.002

(DOI: 10.1016/j.chemphyslip.2017.10.002) Masaki GOTO, Yuya Aoki, Nobutake Tamai and Hitoshi Matsuki :
Effect of pressure on bilayer phase behavior of N-methylated di-O-hexadecylphosphatidylethanolamines: relevance of head-group modification on the bilayer interdigitation,
Biophysical Chemistry, Vol.231, 64-70, 2017.

(要約): 極性基サイズの異なる3種類のエーテル型リン脂質N-メチル化ジヘキサデシルホスファチジルエタノールアミンが形成する二分子膜の高圧相挙動を調査した．極性基サイズの増加と共に相転移温度は段階的に降下したが，相転移熱力学量は極性基サイズにはほとんど依存しなかった．指組み構造ゲル相は，これら脂質二分子膜では常圧，高圧下共に観測されなかった．エステル型リン脂質N-メチル化ジパルミトイルホスファチジルエタノールアミン二分子膜系との結果の比較から，指組み構造形成にはコリン基のような嵩高い極性基が必須であることが明らかとなった．
(キーワード): Bilayer interdigitation / Ether-linked phospholipid / Lipid bilayer membrane / Phase transition / Pressure
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bpc.2017.03.009
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2017.03.009

(DOI: 10.1016/j.bpc.2017.03.009) Hitoshi Matsuki, Shigeru Endo, Ryosuke Sueyoshi, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Thermotropic and barotropic phase transitions on diacylphosphatidylethanolamine bilayer membranes,
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1859, No.7, 1222-1232, 2017.

(要約): 4種類の飽和ホスファチジルエタノールアミン(PE)および2種類の不飽和PEが形成する二分子膜の温度および圧力誘起相転移を調査した．飽和PE二分子膜は熱履歴に依存して水和結晶相/液晶相転移およびゲル相/液晶相(主)転移を示し，長鎖飽和PE二分子膜においては高圧力下でゲル相の安定性が変化した．熱力学量から，飽和PE二分子膜は同鎖長のホスファチジルコリン(PC)二分子膜よりも顕著に安定な水和結晶相を形成することがわかった．他方，不飽和PE二分子膜は，液晶相から逆ヘキサゴナル相への二分子膜/非二分子膜転移を誘起した．熱力学的な考察から，この転移は体積駆動の転移であることを示した．
(キーワード): Differential scanning calorimetry / Lipid bilayer membrane / Phase stability / Phase transition / Phosphatidylethanolamine / Pressure
(徳島大学機関リポジトリ): ● Metadata: 111087
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamem.2017.03.020
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28366514; ● Search Scopus @ Elsevier (PMID): 28366514; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2017.03.020

(徳島大学機関リポジトリ: 111087, DOI: 10.1016/j.bbamem.2017.03.020, PubMed: 28366514) Masaki GOTO, Takuya Endo, Takahiro Yano, Nobutake Tamai, Joachim Kohlbrecher and Hitoshi Matsuki :
Comprehensive characterization of temperature- and pressure-induced bilayer phase transitions for saturated phosphatidylcholines containing longer chain homologs,
Colloids and Surfaces B:Biointerfaces, Vol.128, 389-397, 2015.

(要約): 疎水鎖長が異なる長鎖飽和ジアシルホスファチジルコリン(CnPC: n = 19 - 21)の温度および圧力誘起二分子膜相転移を，特に水和結晶(Lc)相に着目して調査した．C20PCまではLc相を観測することができたが，C21PCでは観測できなかった．得られた結果を過去になされたCnPC同族体のものと比較して，CnPC二分子膜の相挙動を包括的に考察した．結果，CnPC(n = 12 - 22)二分子膜の温度-圧力相図，各種の相転移(副転移，前転移，主転移)に伴う熱力学量のアシル鎖長依存性および圧力誘起指組み構造形成の短鎖および長鎖限界など，CnPC二分子膜相挙動に関する有用な情報を得ることができた．
(キーワード): Bilayer membrane / Diacylphosphatidylcholine / Interdigitation / 相転移 (phase transition) / 高圧力 (high pressure) / Subgel phase
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.colsurfb.2015.02.036
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84938086188

(DOI: 10.1016/j.colsurfb.2015.02.036, Elsevier: Scopus) 松木均, 後藤優樹, 玉井伸岳 :
脂質膜の圧力誘起指組構造形成:疎水鎖長依存性と形成限界,
材料, Vol.63, No.8, 620-623, 2014年.

(要約): We investigated the bilayer interdigitation of diacylphosphatidylcholines (CnPCs) with long acyl chains (n = 19 - 22) under high pressure. The temperature-pressure phase diagrams of the CnPC bilayers were constructed by the methods of differential scanning calorimetry and high-pressure light transmittance. We confirmed the bilayer interdigitation for CnPCs (n = 19 - 21) under high pressure. On the other hand, the C22PC bilayer showed peculiar behavior: two transitions were observed under atmospheric pressure while one transition under high pressure. To identify the low-temperature phase of the C22PC bilayer, we performed small angle neutron scattering measurements under atmospheric pressure and fluorescence measurements under high pressure. From these results, the phase was identified as the interdigitated gel (LβI) phase. Considering our previous results, it turned out that the acyl-chain length of CnPC that exhibits the pressure-induced interdigitation is limited within n = 14 - 21. The present results definitely indicate that the bilayer interdigitation is governed by the hydrophobic interaction as well as the hydrophilic interaction of the PC molecule in the bilayer.
(キーワード): Bilayer phase transition / bilayer phase transition / Lipid bilayers / lipid bilayers / Phase behavior / phase behavior / Pressure-induced interdigitation / pressure-induced interdigitation / Saturated and unsaturated phospholipids / saturated and unsaturated phospholipids
(出版サイトへのリンク): ● Publication site (DOI): 10.2472/jsms.63.620
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282680421512704; ● Summary page in Scopus @ Elsevier: 2-s2.0-84906962918

(DOI: 10.2472/jsms.63.620, CiNii: 1390282680421512704, Elsevier: Scopus) Nobutake Tamai, Takuya Izumikawa, Suguru Fukui, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
How Does Acyl-chain Length Affect Thermotropic Phase Behavior of Saturated Diacylphosphatidylcholine-chloresterol Binary Bilayers?,
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1828, No.11, 2513-2523, 2013.

(要約): Thermotropic phase behavior of diacylphosphatidylcholine (CnPC) cholesterol binary bilayers (n = 14 16) was examined by fluorescence spectroscopy using 6-propionyl-2-(dimethylamino)naphthalene (Prodan) and differential scanning calorimetry. The former technique can detect structural changes of the bilayer in response to the changes in polarity around Prodan molecules partitioned in a relatively hydrophilic region of the bilayer, while the latter is sensitive to the conformational changes of the acyl chains. On the basis of the data from both techniques, we propose possible temperature T cholesterol composition Xch phase diagrams for these binary bilayers. A notable feature of our phase diagrams, including our previous results for C17PC and C18PC, is that there is a peritectic-like point around Xch = 0.15, which can be interpreted as indicating the formation of a 1:6-complex of cholesterol and CnPCs within the binary bilayer irrespective of the acyl chain length. We could give a reasonable explanation for such complex formation using the modified superlattice view. Our results also showed that the Xch value of the abolition of the main transition is almost constant for n = 14 17 (ca. 0.33), while it increases to ca.0.50 for n = 18. By contrast, a biphasic n-dependence of Xch was observed for the abolition of the pretransition, suggesting that there are at least two antagonistic n-dependent factors. We speculate that this could be explained by the enhancement of the van der Waals interaction with increases in n and the weakening of the repulsion between the neighboring headgroups with decreases in n.
(キーワード): コレステロール (cholesterol) / Diacylphosphatidylcholines / Peritectic point / 相図 (phase diagram) / Lateral phase separation / Superlattice
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamem.2013.06.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23791704; ● Search Scopus @ Elsevier (PMID): 23791704; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2013.06.008

(DOI: 10.1016/j.bbamem.2013.06.008, PubMed: 23791704) Nobutake Tamai, Sayuri Kakibe, Tanaka Saeko, Masaki GOTO and Hitoshi Matsuki :
An Attempt to Reveal Viscoelastic Behavior of Lipid Bilayer Membrane by Pressure Perturbation Calorimetry,
High Pressure Research, Vol.33, No.2, 271-277, 2013.

(出版サイトへのリンク): ● Publication site (DOI): 10.1080/08957959.2013.767900
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2013.767900

(DOI: 10.1080/08957959.2013.767900) Nobutake Tamai, Sanae Inazawa, Daiki Fujiwara, Masaki GOTO and Hitoshi Matsuki :
Thermotropic Phase Behavior of Binary Bilayer Membrane of Dipalmitoylphosphatidylcholine and Ergosterol,
Chemistry Letters, Vol.41, No.10, 1087-1089, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1246/cl.2012.1087
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2012.1087

(DOI: 10.1246/cl.2012.1087) Masaki GOTO, Agnieszka Wilk, Kosuke Kataoka, Shirish Chodankar, Nobutake Tamai, Makoto Fukui, Joachim Kohlbrecher, Hiro-O Ito and Hitoshi Matsuki :
Study on the Subgel-Phase Formation Using an Asymmetric Phospholipid Bilayer Membrane by High-Pressure Fluorometry,
Langmuir, Vol.28, No.33, 12191-12198, 2012.

(要約): The myristoylpalmitoylphosphatidylcholine (MPPC) bilayer membrane shows a complicated temperature-pressure phase diagram. The large portion of the lamellar gel (L(β)'), ripple gel (P(β)'), and pressure-induced gel (L(β)I) phases exist as metastable phases due to the extremely stable subgel (L(c)) phase. The stable L(c) phase enables us to examine the properties of the L(c) phase. The phases of the MPPC bilayers under atmospheric and high pressures were studied by small-angle neutron scattering (SANS) and fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan. The SANS measurements clearly demonstrated the existence of the metastable L(β)I phase with the smallest lamellar repeat distance. From a second-derivative analysis of the fluorescence data, the line shape for the L(c) phase under high pressure was characterized by a broad peak with a minimum of ca. 460 nm. The line shapes and the minimum intensity wavelength (λ''(min)) values changed with pressure, indicating that the L(c) phase has highly pressure-sensible structure. The λ''(min) values of the L(c) phase spectra were split into ca. 430 and 500 nm in the L(β)I phase region, which corresponds to the formation of a interdigitated subgel L(c) (L(c)I) phase. Moreover, the phase transitions related to the L(c) phase were reversible transitions under high pressure. Taking into account the fluorescence behavior of Prodan for the L(c) phase, we concluded that the structure of the L(c) phase is highly probably a staggered structure, which can transform into the L(c)I phase easily.
(キーワード): 2-Naphthylamine / Atmospheric Pressure / Cell Membrane / Fluorometry / Gels / Lipid Bilayers / Neutron Diffraction / Phosphatidylcholines / Pressure / Scattering, Small Angle
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/la3020173
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22823885; ● Summary page in Scopus @ Elsevier: 2-s2.0-84865495527

(DOI: 10.1021/la3020173, PubMed: 22823885, Elsevier: Scopus) Saeko Tanaka, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Phase Behavior of Dipalmitoylphosphatidylglycerol Bilayer Membrane in Saline Water under High Pressure,
International Journal of Modern Physics: Conference Series, Vol.6, 727-732, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1142/S2010194512004059
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1142/S2010194512004059

(DOI: 10.1142/S2010194512004059) Nobutake Tamai, Maiko Tsutsui, Masaki GOTO and Hitoshi Matsuki :
Volumetric Study on Dioleoylphosphatidylcholine Bilayer by Pressure Perturbation Calorimetry,
International Journal of Modern Physics: Conference Series, Vol.6, 762-767, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1142/S2010194512004114
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1142/S2010194512004114

(DOI: 10.1142/S2010194512004114) Nobutake Tamai, Yuko Nambu, Saeko Tanaka, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Volumetric characterization of ester- and ether-linked lipid bilayers by pressure perturbation calorimetry and densitometry,
Colloids and Surfaces B:Biointerfaces, Vol.92, No.1, 232-239, 2012.

(要約): We investigated the thermotropic volume behavior of dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC) and dihexadecylphosphatidylcholine (DHPC) membranes using pressure perturbation calorimetry (PPC) and densitometry. The ln φ(2) vs temperature curves (φ(2): apparent molar volume of phospholipid) obtained from the PPC data using an analysis method that we developed agreed with the results from the density measurements for these lipids within the relative difference of about 0.62%. From those curves, the volume changes with the main transition were estimated at 18.0±0.49, 23.5±2.33 and 23.0±0.33 cm(3) mol(-1) for DMPC, DPPC and DHPC, respectively. For DPPC and DMPC, the average volume per methylene group of the hydrocarbon chains v(CH2) calculated by referring to the procedure by Nagle and Wilkinson was consistent with the previous result, which indicates that the DPPC bilayer in the gel state has denser hydrophobic bilayer core than the DMPC bilayer. For DHPC, the volume of the headgroup region v(H) was calculated to be 244 Å(3) by assuming that v(CH2) of DHPC equals that of DPPC above 45°C. This value was comparable to that of DPPC when the volume of the carbonyl groups was considered, which may signify that there is no significant conformational difference in the polar headgroups of both phospholipids. However, it was suggested from the consideration on v(H) of DHPC at 20°C that expansion of the headgroup region should occur as the interdigitated structure is formed, which means some conformational change of the headgroup region is induced by the interdigitation.
(キーワード): 1,2-Dipalmitoylphosphatidylcholine / Calorimetry / Densitometry / Dimyristoylphosphatidylcholine / Esters / Ether, Ethyl / Lipid Bilayers / Phospholipid Ethers / Pressure / Temperature
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.colsurfb.2011.11.055
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22221457; ● Search Scopus @ Elsevier (PMID): 22221457; ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2011.11.055

(DOI: 10.1016/j.colsurfb.2011.11.055, PubMed: 22221457) Saeko Tanaka, Nobutake Tamai, Masaki GOTO, Shoji Kaneshina and Hitoshi Matsuki :
Morphological Change of Vesicle Particle can Produce a Peculir Stepwise Transition in Dipalmitoylphosphatidylglycerol Bilayer at High NaCl Concentration,
Chemistry Letters, Vol.41, No.3, 304-306, 2012.

(出版サイトへのリンク): ● Publication site (DOI): 10.1246/cl.2012.304
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1360283694088387200; ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2012.304

(DOI: 10.1246/cl.2012.304, CiNii: 1360283694088387200) Hitoshi Matsuki, Masaki GOTO, Masataka Kusube and Nobutake Tamai :
Imaging of Phosphatidylcholine Bilayers by a High-Pressure Fluorescence Technique: Detection of the Packing Difference,
Bulletin of the Chemical Society of Japan, Vol.84, No.12, 1329-1335, 2011.

(要約): The packing states in phosphatidylcholine (PC) bilayers under high pressure were studied from fluorescence measurements using a polarity-sensitive probe Prodan. By using the Prodan fluorescence data, three-dimensional image plots, which can visually evaluate the bilayer packing, were constructed for the C16PC bilayer. It turned out from the shape of contours in the image plot constructed by the second-derivative spectra that the Prodan molecules can be distributed into multiple sites in the bilayer and move around the head-group region depending on the phase state. Furthermore, comparing the plot to those for the C17PC and C18PC bilayers, the slight difference in hydrophobicity among the PC molecules produced a marked difference in quantity of the Prodan molecules distributed among the bilayer gel, nonbilayer gel, and liquid crystalline phases of the PC membranes. The present study shows that the imaging using Prodan is useful for the evaluation of lipid bilayer packing.
(キーワード): General Chemistry
(出版サイトへのリンク): ● Publication site (DOI): 10.1246/bcsj.20110229
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1360283694073547136; ● Search Scopus @ Elsevier (DOI): 10.1246/bcsj.20110229

(DOI: 10.1246/bcsj.20110229, CiNii: 1360283694073547136) Tselmeg Baatarjav, Kosuke Kataoka, Rebekah S. Gilbert, Yutaka Terao, Makoto Fukui, Masaki GOTO, Shigetada Kawabata, Masato Yamamoto, Kohtaro Fujihashi and Hiro-O Ito :
Mucosal immune features to phosphorylcholine by nasal Flt3 ligand cDNA-based vaccination,
Vaccine, Vol.29, No.34, 5747-5757, 2011.

(要約): Phosphorylcholine (PC) is an immunodominant epitope in some pathogens including Streptococcus pneumoniae and it is well-known that PC-specific antibodies (Abs) play a key role in the induction of protective immunity against pneumococcal infection. In this study, we examined whether nasal administration of DNA plasmid encoding Flt3 ligand gene (pFL) as a mucosal adjuvant plus PC-conjugated keyhole limpet hemocyanin (PC-KLH), would elicit PC-specific immune responses, and characterized mucosal immune responses to PC induced by this nasal vaccination. Nasal immunization with pFL plus PC-KLH enhanced induction of PC-specific IgA and IgM Abs in airway secretions when compared with mice given PC-KLH with or without empty plasmid gene (pORF) as controls; in addition to the mucosal immune responses, PC-specific immune responses in serum were also induced. Furthermore, the mucosal and serum IgA and IgM Abs in mice given pFL plus PC-KLH nasally, exhibited high-specificity for the PC molecule. Of interest, the PC-specific Abs bound dose-dependently to anti-T15 idiotype (AB1-2). Thus, the inhibition of S. pneumoniae colonization on the nasal cavity and lungs after nasal challenge with the live organism was significantly elicited in mice immunized with pFL plus PC-KLH compared to that of mice immunized with antigen with pORF. Taken together, these findings show that nasal administration of pFL with PC-KLH elicited T15-like anti-PC IgA and IgM Abs in the respiratory tracts, and further attenuated S. pneumoniae colonization on the respiratory tracts. Nasal administration of Flt3 ligand cDNA with PC may contribute to the development of nasal vaccination for prevention of S. pneumoniae infection.
(キーワード): Administration, Intranasal / Administration, Mucosal / Animals / Antibodies, Bacterial / Bacterial Vaccines / DNA, Complementary / Hemocyanin / Immunity, Mucosal / Immunoglobulin A / Immunoglobulin M / Membrane Proteins / Mice / Mice, Inbred C57BL / Phosphorylcholine / Plasmids / Pneumococcal Infections / Streptococcus pneumoniae / Vaccines, DNA
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.vaccine.2011.05.097
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21683111; ● Summary page in Scopus @ Elsevier: 2-s2.0-79960560899

(DOI: 10.1016/j.vaccine.2011.05.097, PubMed: 21683111, Elsevier: Scopus) Masaki GOTO, Shunsuke Ishida, Yuka Ito, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Thermotropic and Barotropic Phase Transitions of Dialkyldimethylammonium Bromide Bilayer Membranes: Effect of Chain Length,
Langmuir, Vol.27, No.10, 5824-5831, 2011.

(要約): The bilayer phase transitions of dialkyldimethylammonium bromides (2C(n)Br; n = 12, 14, 16) were observed by differential scanning calorimetry and high-pressure light-transmittance measurements. Under atmospheric pressure, the 2C(12)Br bilayer membrane underwent the stable transition from the lamellar crystal (L(c)) phase to the liquid crystalline (L(α)) phase. The 2C(14)Br bilayer underwent the main transition from the metastable lamellar gel (L(β)) phase to the metastable L(α) phase in addition to the stable L(c)/L(α) transition. For the 2C(16)Br bilayer, moreover, three kinds of phase transitions were observed: the metastable main transition, the metastable transition from the metastable lamellar crystal (L(c(2))) phase to the metastable L(α) phase, and the stable lamellar crystal (L(c(1)))/L(α) transition. The temperatures of all the phase transitions elevated almost linearly with increasing pressure. The temperature (T)-pressure (p) phase diagrams of the 2C(12)Br and 2C(14)Br bilayers were simple, but that of the 2C(16)Br bilayer was complex; that is, the T-p curves for the metastable main transition and the L(c(2))/L(α) transition intersect at ca. 25 MPa, which means the inversion of the relative phase stability between the metastable phases of L(β) and L(c(2)) above and below the pressure. Moreover, the T-p curve of the L(c(2))/L(α) transition was separated into two curves under high pressure, and as a result, the pressure-induced L(c(2P)) phase appeared in between. Thermodynamic quantities for phase transitions of the 2C(n)Br bilayers increased with an increase in alkyl-chain length. The chain-length dependence of the phase-transition temperature for all kinds of transitions observed suggests that the stable L(c(1))/L(α) transition incorporates the metastable L(c(2))/L(α) transition in the bilayers of 2C(n)Br with shorter alkyl chains, and the main-transition of the 2C(12)Br bilayer would occur at a temperature below 0 °C.
(キーワード): Atmospheric Pressure / Cell Membrane / Light / Lipid Bilayers / Phase Transition / Quaternary Ammonium Compounds / Surface-Active Agents / Temperature / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/la200323h
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21520909; ● Search Scopus @ Elsevier (PMID): 21520909; ● Search Scopus @ Elsevier (DOI): 10.1021/la200323h

(DOI: 10.1021/la200323h, PubMed: 21520909) Masaki GOTO, Takayuki Matsui, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Prodan fluorescence detects the bilayer packing of asymmetric phospholipids,
Colloids and Surfaces B:Biointerfaces, Vol.84, No.1, 55-62, 2011.

(要約): The bilayer phase behavior of asymmetric phospholipids, palmitoylstearoylphosphatidylcholine (PSPC) and stearoylpalmitoylphosphatidylcholine (SPPC), with different vesicle sizes (large multilamellar vesicle (LMV) and giant multilamellar vesicle (GMV)) was investigated by fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan under high pressure. The results were compared with those of a symmetric phospholipid, diheptadecanoyl PC (C17PC). The difference in phase transitions of the PSPC and SPPC bilayers and in thermodynamic quantities of the transitions was hardly observed between LMV and GMV as the case of the C17PC bilayer. On the other hand, the Prodan fluorescence showed clear differences between LMV and GMV of the asymmetric PC bilayers. From the second derivative of Prodan fluorescence spectra, the three dimensional image plots in which we can clearly see the location of Prodan in the bilayer membrane as blue valleys were constructed for LMV and GMV under high pressure. We revealed from the plots that the bilayer packing is significantly dependent on not only the vesicle size but also the acyl-chain asymmetry of PC molecule in addition to the phase states. It was found that the packing of the gel phases of the asymmetric PC bilayers is weaker than that of the symmetric PC bilayer, and the size of vesicle affects the packing of the interdigitated gel phase the most markedly among three gel phases. This study suggests that the Prodan molecules can detect the effect of vesicle size on the phase states for the asymmetric PC bilayers, and they become a useful indicator for various membrane properties, especially bilayer interdigitation.
(キーワード): 2-Naphthylamine / Fluorescence / Fluorescent Dyes / Lipid Bilayers / Models, Biological / Phase Transition / Phospholipids / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.colsurfb.2010.12.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21232926; ● Search Scopus @ Elsevier (PMID): 21232926; ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2010.12.015

(DOI: 10.1016/j.colsurfb.2010.12.015, PubMed: 21232926) Masaki GOTO, Agnieszka Wilk, Akira Kazama, Shirish Chodankar, Joachim Kohlbrecher and Hitoshi Matsuki :
Chain elongation of diacylphosphatidylcholine induces fully bilayer interdigitation under atmospheric pressure,
Colloids and Surfaces B:Biointerfaces, Vol.84, No.1, 44-48, 2011.

(要約): The phase transitions of dibehenoylphosphatidylcholine (C22PC) bilayer membrane were observed by differential scanning calorimetry under atmospheric pressure and light-transmittance measurements under high pressure. The constructed temperature-pressure phase diagram suggests that the gel phase at low temperatures is the interdigitated gel phase. To confirm the phase state, we performed small-angle neutron scattering and fluorescence measurements using a polarity-sensitive probe Prodan for the C22PC bilayer membrane under atmospheric pressure. The peaks obtained in both measurements clearly showed the characteristic patterns of the fully interdigitated gel phase. Taking into account of previous studies on the gel phase for long-chain PC bilayers under atmospheric pressure and our studies on the pressure-induced bilayer interdigitaion of diacyl-PCs, it turned out that the interdigitation of diacyl-PC bilayer membranes occurs when the carbon number of acyl chain reaches at least 22. The present study revealed that the interdigitation of PC bilayer membranes occurs not only by weakening the attractive force of polar head groups but also by strengthening the cohesive force of acyl chains. When dominating the force of acyl chains, the interdigitation can be induced even in a diacyl-PC bilayer membrane by only hydration under atmospheric pressure.
(キーワード): Atmospheric Pressure / Lipid Bilayers / Models, Biological / Phosphatidylcholines
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.colsurfb.2010.12.010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21216568; ● Search Scopus @ Elsevier (PMID): 21216568; ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2010.12.010

(DOI: 10.1016/j.colsurfb.2010.12.010, PubMed: 21216568) Masaki GOTO, Yuka Ito, Shunsuke Ishida, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Hydrostatic Pressure Reveals Bilayer Phase Behavior of Dioctadecyldimethylammonium Bromide and Chloride,
Langmuir, Vol.27, No.5, 1592-1598, 2011.

(要約): Bilayer phase transitions of dioctadecyldimethylammonium bromide (2C(18)Br) and chloride (2C(18)Cl) were observed by differential scanning calorimetry and high-pressure light-transmittance measurements. The 2C(18)Br bilayer membrane showed different kinds of transitions depending on preparation methods of samples under atmospheric pressure. Under certain conditions, the 2C(18)Br bilayer underwent three kinds of transitions, the metastable transition from the metastable lamellar crystal (L(c(2))) phase to the metastable lamellar gel (L(β)) phase at 35.4 °C, the metastable main transition from the metastable L(β) phase to the metastable liquid crystalline (L(α)) phase at 44.5 °C, and the stable transition from the stable lamellar crystal (L(c(1))) phase to the stable L(α) phase at 52.8 °C. On the contrary, the 2C(18)Cl bilayer underwent two kinds of transitions, the stable transition from the stable L(c) phase to the stable L(β) phase at 19.7 °C and the stable main transition from the stable L(β) phase to the stable L(α) phase at 39.9 °C. The temperatures of the phase transitions of the 2C(18)Br and 2C(18)Cl bilayers were almost linearly elevated by applying pressure. It was found from the temperature (T)-pressure (p) phase diagram of the 2C(18)Br bilayer that the T-p curves for the main transition and the L(c(1))/L(α) transition intersect at ca. 130 MPa because of the larger slope of the former transition curve. On the other hand, the T-p phase diagram of the 2C(18)Cl bilayer took a simple shape. The thermodynamic properties for the main transition of the 2C(18)Br and 2C(18)Cl bilayers were comparable to each other, whereas those for the L(c(1))/L(α) transition of the 2C(18)Br bilayer showed considerably high values, signifying that the L(c(1)) phase of the 2C(18)Br bilayer is extremely stable. These differences observed in both bilayers are attributable to the difference in interaction between a surfactant and its counterion.
(キーワード): Atmospheric Pressure / Hydrostatic Pressure / Phase Transition / Quaternary Ammonium Compounds / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/la104552z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21261314; ● Search Scopus @ Elsevier (PMID): 21261314; ● Search Scopus @ Elsevier (DOI): 10.1021/la104552z

(DOI: 10.1021/la104552z, PubMed: 21261314) Nobutake Tamai, Yuko Nambu, Maiko Tsutsui, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Application of pressure perturbation calorimetry to the aqueous system of phospholipid vesicle dispersion,
High Pressure Research, Vol.30, No.4, 490-498, 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1080/08957959.2010.526115
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2010.526115

(DOI: 10.1080/08957959.2010.526115) Hitoshi Matsuki, Makoto Nishimoto, Kaori Tada, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Packing state in bilayer membranes of diacylphosphatidylcholines with varying acyl chain lengths under high pressure,
High Pressure Research, Vol.30, No.4, 475-482, 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1080/08957959.2010.526114
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1080/08957959.2010.526114

(DOI: 10.1080/08957959.2010.526114) Masaki GOTO, Hiroshi Sawaguchi, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Effect of Vesicle Size on the Prodan Fluorescence in Diheptadecanoylphosphatidylcholine Bilayer Membrane under Atmospheric and High Pressures,
Langmuir, Vol.26, No.16, 13377-13384, 2010.

(要約): The bilayer phase behavior of diheptadecanoylphosphatidylcholine (C17PC) with different vesicle sizes (large multilamellar vesicle (LMV) and giant multilamellar vesicle (GMV)) was investigated by fluorescence spectroscopy using a polarity-sensitive fluorescent probe Prodan under atmospheric and high pressures. The difference in phase transitions and thermodynamic quantities of the transition was hardly observed between LMV and GMV used here. On the contrary, the Prodan fluorescence in the bilayer membranes changed depending on the size of vesicles as well as on the phase states. From the second derivative of fluorescence spectra, the three-dimensional image plots in which we can see the location of Prodan in the bilayer membrane as blue valleys were constructed for LMV and GMV under atmospheric pressure. The following characteristic behavior was found: (1) the Prodan molecules in GMV can be distributed to not only adjacent glycerol backbone region, but also near bulk-water region in the lamellar gel or ripple gel phase; (2) the blue valleys of GMV became deeper than those of LMV because of the greater surface density of the Prodan molecules per unit area of GMV than LMV; (3) the liquid crystalline phase of the bilayer excludes the Prodan molecules to a more hydrophilic region at the membrane surface with an increase in vesicle size; (4) the accurate information as to the phase transitions is gradually lost with increasing vesicle size. Under the high-pressure condition, the difference in Prodan fluorescence between LMV and GMV was essentially the same as the difference under atmospheric pressure except for the existence of the pressure-induced interdigitated gel phase. Further, we found that Prodan fluorescence spectra in the interdigitated gel phase were especially affected by the size of vesicles. This study revealed that the Prodan molecules can move around the headgroup region by responding not only to the phase state but also to the vesicle size, and they become a useful membrane probe, detecting important membrane properties such as the packing stress.
(キーワード): 2-Naphthylamine / Fluorescent Dyes / Lipid Bilayers / Phosphatidylcholines / Pressure / Spectrometry, Fluorescence / Temperature / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/la100871z
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20695581; ● Search Scopus @ Elsevier (PMID): 20695581; ● Search Scopus @ Elsevier (DOI): 10.1021/la100871z

(DOI: 10.1021/la100871z, PubMed: 20695581) Masataka Kusube, Takumi Nishino, Yuki Nishikawa, Masaki GOTO, Hitoshi Matsuki and Hitoshi Iwahashi :
Recovery of Tobacco BY-2 Cells After Treatment of High Hydrostatic Pressure,
Annals of the New York Academy of Sciences, Vol.1189, 139-142, 2010.

(要約): The recovery of Nicotiana tabacum L. cv. Bright Yellow 2 (BY-2) cells in Linsmaire and Skoog medium after treatment at high hydrostatic pressure was investigated using an Evans Blue staining method to discriminate live from dead cells. The survival of BY-2 cells just after the high-pressure treatment at 5 degrees C and 25 degrees C decreased abruptly at pressures higher than 50 MPa and 100 MPa, respectively. Furthermore, almost all of the BY-2 cells treated at 5 degrees C and 25 degrees C recovered pressures below 25 MPa and 75 MPa, respectively. However, no BY-2 cells recovered at pressures above 100 MPa at either temperature.
(キーワード): Biotechnology / Cell Line / Cell Proliferation / Cell Survival / Hydrostatic Pressure / Preservation, Biological / Temperature / Tobacco
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1749-6632.2009.05185.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233380; ● Search Scopus @ Elsevier (PMID): 20233380; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05185.x

(DOI: 10.1111/j.1749-6632.2009.05185.x, PubMed: 20233380) Masaki GOTO, Masataka Kusube, Makoto Nisimoto, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Pressure Study on Symmetric and Asymmetric Phospholipid Bilayers: Effect of Vesicle Size on Prodan Fluorescence,
Annals of the New York Academy of Sciences, Vol.1189, 68-76, 2010.

(要約): The bilayer phase behavior of symmetric and asymmetric phosphatidylcholines (PCs), 1,2-diheptadecanoyl-PC (C17PC), 1-palmitoyl-2-stearoyl-PC (PSPC), and 1-stearoyl-2-palmitoyl-PC (SPPC), with different vesicle sizes were investigated by a high-pressure fluorescence method using the polarity-sensitive fluorescent probe Prodan. The second derivative of fluorescence spectra for all the PCs of small-sized vesicle showed four minima characteristic of four membrane states on the spectra irrespective of the acyl-chain symmetry, whereas those of large-sized vesicle had one more minimum originating from the most hydrophilic site at the membrane surface. These findings indicate that Prodan molecules can distribute into multiple sites in the bilayer and move around the head-group region depending on the vesicle size. The behavior of the spectra in the SPPC bilayer suggested that the interdigitated gel phase had a less polar "pocket" formed by a space between uneven terminal methyl ends of the sn-1 and sn-2 chains. It turned out that the curvature of vesicles affects the distribution of the Prodan molecules in all phases, more particularly in the interdigitated gel phase.
(キーワード): 2-Naphthylamine / Biotechnology / Fluorescent Dyes / Hydrostatic Pressure / Lipid Bilayers / Particle Size / Phase Transition / Phosphatidylcholines / Phosphatidylserines / Phospholipids / Spectrometry, Fluorescence
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1749-6632.2009.05202.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233370; ● Search Scopus @ Elsevier (PMID): 20233370; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05202.x

(DOI: 10.1111/j.1749-6632.2009.05202.x, PubMed: 20233370) Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Pressure Effect on the Bilayer Phase Transition of Asymmetric Lipids with an Unsaturated Acyl Chain,
Annals of the New York Academy of Sciences, Vol.1189, 77-85, 2010.

(要約): The bilayer phase transitions of mixed-chain lipids with monounsaturated acyl chain in the sn-2 position, 1-myristoyl-2-oleoyl-sn-glycero-3-phosphocholine (MOPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC), and with a polyunsaturated acyl chain in the sn-2 position, 1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine (SLPC), 1-stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholine (SAPC), and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (SDPC), were observed by differential scanning calorimetry (DSC) under ambient pressure and by light-transmittance measurements under high pressure. The DSC thermogram for each lipid bilayer showed only one transition between the lamellar gel and liquid crystalline phases. The introduction of one or two cis double bonds into the sn-2 acyl chain caused the significant depression of the main-transition temperature and an obvious decrease of enthalpy and volume changes associated with the transition. These features are attributable to loose packing of saturated and unsaturated acyl chains in the bilayer gel phase. The existence of four or six double bonds in the sn-2 chain produced no further decrease in the transition temperature, and in fact six double bonds caused a slight increase in the transition temperature. Thermodynamic properties associated with the bilayer phase transition were discussed.
(キーワード): Biotechnology / Calorimetry, Differential Scanning / Fatty Acids, Monounsaturated / Fatty Acids, Unsaturated / Gels / Hydrostatic Pressure / Lipid Bilayers / Molecular Structure / Phase Transition / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1749-6632.2009.05203.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20233371; ● Search Scopus @ Elsevier (PMID): 20233371; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1749-6632.2009.05203.x

(DOI: 10.1111/j.1749-6632.2009.05203.x, PubMed: 20233371) Ismail Ismaliza Mohd Nor, Tsuyoshi Yuasa, Keizo Yuasa, Yuko Nambu, Makoto Nishimoto, Masaki GOTO, Hitoshi Matsuki, Masahiro Inoue, Masami Nagahama and Akihiko Tsuji :
A critical role for highly conserved Glu⁶¹⁰ residue of oligopeptidase B Tryoanosoma brucei in thermal stability,
The Journal of Biochemistry, Vol.147, No.2, 201-211, 2010.

(要約): Oligopeptidase B from Trypanosoma brucei (Tb OPB) is a virulence factor and therapeutic target in African sleeping sickness. Three glutamic acid residues at positions 607, 609 and 610 of the catalytic domain are highly conserved in the OPB subfamily. In this study, the roles of Glu(607), Glu(609) and Glu(610) in Tb OPB were investigated by site-directed mutagenesis. A striking effect on k(cat)/K(m) was obtained following mutation of Glu(607) to glutamine. In contrast, the heat stability of Tb OPB decreased markedly following the single mutation of Glu(610) to glutamine, although this mutation had significantly less effect on catalytic properties compared with the Glu(607) mutation. Although no differences were found in the tertiary and secondary structures between wild-type (WT) OPB and the E610Q mutant prior to heat treatment, the E610Q mutant is inactivated more rapidly than WT OPB following heat treatment in a manner correlating with its attendant structural changes. Trypsin digestion showed that the boundary regions between the beta-propeller and catalytic domain of the E610Q mutant are unfolded with heat treatment. It is concluded that Glu(607) is essential for the catalytic activity of Tb OPB and that Glu(610) plays a critical role in stabilization rather than catalytic activity despite their close proximity.
(キーワード): Adrenocorticotropic Hormone / Animals / Calorimetry, Differential Scanning / Circular Dichroism / Enzyme Activation / Enzyme Stability / Glucagon / Glutamic Acid / Hot Temperature / Mutagenesis, Site-Directed / Mutation / Protozoan Proteins / Salts / Serine Endopeptidases / Trypanosoma brucei brucei / Trypsin / Urea
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvp156
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19819899; ● Search Scopus @ Elsevier (PMID): 19819899; ● Search Scopus @ Elsevier (DOI): 10.1093/jb/mvp156

(DOI: 10.1093/jb/mvp156, PubMed: 19819899) Agnieszka Broniec, Masaki GOTO and Hitoshi Matsuki :
A Peculiar Phase Transition of Plasmalogen Bilayer Membrane under High Pressure,
Langmuir, Vol.25, No.19, 11265-11268, 2009.

(要約): The bilayer phase transition of plasmalogen, monounsaturated plasmenylcholine 1-O-1'-(Z)-octadecenyl-2-oleoyl-sn-glycero-3-phosphocholine (Plg-SOPC), was examined by differential scanning calorimetry, high-pressure transmittance, and fluorescence techniques. The bilayer properties of Plg-SOPC such as the temperature-pressure phase diagram, the thermodynamic quantities of the transition, and the location of a fluorescent membrane probe in the bilayer, were compared with those of a similar phospholipid 1-stearoyl-2-oleoyl-phosphatidylcholine (SOPC). It turned out that a vinyl-ether bond in the sn-1 position of the glycerol backbone in the Plg-SOPC molecule produces a peculiar phase transition under high pressure and significantly affects the membrane properties.
(キーワード): Cell Membrane / Light / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Plasmalogens / Pressure / Scattering, Radiation / Temperature
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/la902503n
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19697955; ● Search Scopus @ Elsevier (PMID): 19697955; ● Search Scopus @ Elsevier (DOI): 10.1021/la902503n

(DOI: 10.1021/la902503n, PubMed: 19697955) Masaki GOTO, Shunsuke Ishida, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Chain asymmetry alters thermotropic and barotropic properties of phospholipid bilayer membranes,
Chemistry and Physics of Lipids, Vol.161, No.2, 65-76, 2009.

(要約): The alignment of the sn-1 and sn-2 acyl chains at the terminal methyl ends generally produces significant influence on the thermodynamic properties of the bilayer phase transitions. We investigated the bilayer phase behavior of asymmetric phospholipids, myristoylpalmitoylphosphatidylcholine and palmitoylmyristoylphosphatidylcholine, by high-pressure light-transmittance and Prodan-fluorescence techniques and differential scanning calorimetry. Constructed temperature-pressure phase diagrams revealed that no stable Lbeta' phase can exist in the whole pressure range because of the formation of the most stable Lc phase. Nevertheless, the pretransition, the detection of which is severely hampered by the exceptionally prompt formation of the Lc phase, was successfully observed. Moreover, the effect of the total and difference of the sn-1 and sn-2 acyl chain lengths on minimal interdigitation pressure (MIP) was summarized in a MIP vs. chain-length inequivalence parameter plot, where the effect was proved to be classified in three zones depending on the alignment of both terminal methyl ends.
(キーワード): 2-Naphthylamine / Calorimetry, Differential Scanning / Cholesterol / Hydrostatic Pressure / Lipid Bilayers / Phase Transition / Phospholipids / Pressure / Structure-Activity Relationship / Temperature / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.chemphyslip.2009.07.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19616525; ● Search Scopus @ Elsevier (PMID): 19616525; ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2009.07.003

(DOI: 10.1016/j.chemphyslip.2009.07.003, PubMed: 19616525) Kaori Tada, Eri Miyazaki, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Barotropic and thermotropic bilayer phase behavior of positional isomers of unsaturated mixed-chain phosphatidylcholines,
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1788, No.5, 1056-1063, 2009.

(要約): The bilayer phase transitions of six kinds of mixed-chain phosphatidylcholines (PCs) with an unsaturated acyl chain in the sn-1 or sn-2 position, 1-oleoyl-2-stearoyl- (OSPC), 1-stearoyl-2-oleoyl- (SOPC), 1-oleoyl-2-palmitoyl- (OPPC), 1-palmitoyl-2-oleoyl- (POPC), 1-oleoyl-2-myristoyl- (OMPC) and 1-myristoyl-2-oleoyl-sn-glycero-3-phosphocholine (MOPC), were observed by means of differential scanning calorimetry (DSC) and high-pressure light transmittance measurements. Bilayer membranes of SOPC, POPC and MOPC with an unsaturated acyl chain in the sn-2 position exhibited only one phase transition, which was identified as the main transition between the lamellar gel (L(beta)) and liquid crystalline (L(alpha)) phases. On the other hand, the bilayer membranes of OSPC, OPPC and OMPC with an unsaturated acyl chain in the sn-1 position exhibited not only the main transition but also a transition from the lamellar crystal (L(c)) to the L(beta) (or L(alpha)) phase. The stability of their gel phases was markedly affected by pressure and chain length of the saturated acyl chain in the sn-2 position. Considering the effective chain lengths of unsaturated mixed-chain PCs, the difference in the effective chain length between the sn-1 and sn-2 acyl chains was proven to be closely related to the temperature difference of the main transition. That is, a mismatch of the effective chain length promotes a temperature difference of the main transition between the positional isomers. Anomalously small volume changes of the L(c)/L(alpha) transition for the OPPC and OMPC bilayers were found despite their large enthalpy changes. This behavior is attributable to the existence of a cis double bond and to significant inequivalence between the sn-1 and sn-2 acyl chains, which brings about a small volume change for chain melting due to loose chain packing, corresponding to a large partial molar volume, even in the L(c) phase. Further, the bilayer behavior of unsaturated mixed-chain PCs containing an unsaturated acyl chain in the sn-1 or sn-2 position was well explained by the chemical-potential diagram of a lipid in each phase.
(キーワード): Biophysical Phenomena / Calorimetry, Differential Scanning / Ethylene Glycol / Isomerism / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Pressure / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamem.2009.02.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19233121; ● Summary page in Scopus @ Elsevier: 2-s2.0-65249097838

(DOI: 10.1016/j.bbamem.2009.02.008, PubMed: 19233121, Elsevier: Scopus) Makoto Nishimoto, Takashi Hata, Masaki GOTO, Nobutake Tamai, Shoji Kaneshina, Hitoshi Matsuki and Issaku Ueda :
Interaction modes of long-chain fatty acids in dipalmitoylphosphatidylcholine bilayer membrane: contrast to mode of inhalation anesthetics,
Chemistry and Physics of Lipids, Vol.158, No.2, 71-80, 2009.

(要約): The effects of long-chain fatty acids (four saturated and two unsaturated fatty acids, one derivative) on phase transitions of dipalmitoylphosphatidylcholine (DPPC) bilayer membranes were examined in the low concentration region, and the results were compared with those for an inhalation anesthetic. The effects of all fatty acids on the pre- and main-transition temperatures of the DPPC bilayer membrane appeared in the concentration range of microM order while that of the anesthetic appeared in the mM order. The appearance modes of these ligand actions were significantly different from one another. The three differential partition coefficients of the ligands between two phases of the DPPC bilayer membrane were evaluated by applying the thermodynamic equation to the variation of the phase-transition temperatures. The DPPC bilayer membranes showed the different receptivity for the ligands; the saturated fatty acids had an affinity for gel phase whereas unsaturated fatty acids and an anesthetic had an affinity for liquid-crystalline phase to the contrary. In particular, the receptivity for the ligands in the gel phase markedly changed depending on kinds of ligands. The interaction modes between the DPPC and fatty acid molecules in the gel phase were considered from the hexagonal lattice model. The disappearance compositions of the pretransition by the fatty acids coincided with the compositions at which the membrane is all covered by the units in each of which two fatty acids molecules are regularly distributed in the hexagonal lattice in a different way, and the distribution depended on the chain length and existence of a double bond for the fatty acids. The interpretation did not hold for the case of the anesthetic at all, which proved that a number of anesthetic molecules act the surface region of the bilayer membrane nonspecifically. The present study clearly implies that DPPC bilayer membranes have high ability to recognize kinds of ligand molecules and can discriminate among them with specific interaction by the membrane states.
(キーワード): 1,2-Dipalmitoylphosphatidylcholine / Anesthetics / Calorimetry, Differential Scanning / Fatty Acids / Lipid Bilayers / Phase Transition
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.chemphyslip.2009.02.003
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19428351; ● Search Scopus @ Elsevier (PMID): 19428351; ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2009.02.003

(DOI: 10.1016/j.chemphyslip.2009.02.003, PubMed: 19428351) Kaori Tada, Kensuke Saito, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
High-pressure study on bilayer phase behavior of oleoylmyristoyl- and myristoyloleoyl-phosphatidylcholines,
Biophysical Chemistry, Vol.138, No.1-2, 36-41, 2008.

(要約): We investigated the thermotropic and barotropic bilayer phase behavior of 1-myristoyl-2-oleoyl-sn-glycero-3-phosphocholine (MOPC) and 1-oleoyl-2-myristoyl-sn-glycero-3-phosphocholine (OMPC) by means of the differential scanning calorimetry (DSC) and high-pressure light-transmittance technique. Water could be used as a solvent for measurements at high pressures because of the elevation of the transition temperatures above 0 degrees C by pressurization, whereas aqueous 50 wt.% ethylene glycol solution was used mainly for those at low pressures. Only one phase transition was observed in the DSC thermogram of the MOPC bilayer membrane as an endothermic peak, and also observed at high pressures as an abrupt change of the light-transmittance. The transition was assigned as a main transition between the lamellar gel (L(beta)) and liquid-crystalline (L(alpha)) phases on the basis of the values of enthalpy change (DeltaH) and slope of the transition temperature with respect to pressure (dT/dP). The DSC thermogram of the OMPC bilayer membrane similarly showed a single endothermic peak but two kinds of phase transitions were observed at different temperatures in the light-transmittance profile at high pressures. The extrapolation of the lower-temperature transition in the high-pressure range to an ambient pressure coincided with the transition observed in the DSC thermogram. This transition was identified as a transition between the lamellar crystal (L(c)) and L(alpha) (or L(beta)) phases from the DeltaH and dT/dP values. The higher-temperature transition, appearing only at high pressures, was identified as the L(beta)/L(alpha) transition considering the topological resemblance of its temperature-pressure phase diagram as that of the dioleoylphosphatidylcholine bilayer membrane. The phase diagram of the OMPC bilayer membrane demonstrated that the L(beta) phase cannot exist at pressures below ca. 190 MPa while it can exist stably in a finite temperature range at pressures above the pressure.
(キーワード): Calorimetry, Differential Scanning / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Pressure / Temperature / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bpc.2008.08.009
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18804320; ● Search Scopus @ Elsevier (PMID): 18804320; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2008.08.009

(DOI: 10.1016/j.bpc.2008.08.009, PubMed: 18804320) Nobutake Tamai, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Lateral phase separation in cholesterol/diheptadecanoylphosphatidylcholine binary bilayer membrane,
Colloids and Surfaces B:Biointerfaces, Vol.65, No.2, 213-219, 2008.

(要約): We investigated the phase behavior of cholesterol/diheptadecanoylphosphatidylcholine (C17:0-PC) binary bilayer membrane as a function of the cholesterol composition (X(ch)) by fluorescence spectroscopy using 6-propionyl-2-(dimethylamino)naphthalene (Prodan) and differential scanning calorimetry (DSC). The fluorescence spectra showed that the wavelength at the maximum intensity (lambda(max)) changed depending on the bilayer state: ca. 440 nm for the lamellar gel (L'(beta) or L(beta)) and the liquid ordered (L(o)) phases and ca. 490 nm for the liquid-crystalline (L(alpha)) phase. The transition temperatures were determined from the temperature dependence of lambda(max) and endothermic peaks of the DSC thermograms. Both measurements showed that the pre- and main transition disappear around X(ch)=0.05 and 0.30, respectively. The constructed temperature-X(ch) phase diagram resembled a typical phase diagram for a eutectic binary mixture containing a peritectic point. The presence of a peritectic point at X(ch)=0.15 suggested that a complex of cholesterol and C17:0-PC is stoichiometrically formed in the gel phase. Consideration based on the hexagonal lattice model revealed that the compositions of 0.05 and 0.15 correspond to the bilayer states where cholesterol molecules are regularly distributed in different ways. The former is nearly equal to the composition for the membrane occupied entirely with Units (1:18), composed of a cholesterol and 18 surrounding C17:0-PC molecules within the next-next nearest neighbor sites. The latter is represented by a Unit (1:6), including a cholesterol and 6 surrounding C17:0-PC molecules. Further, the disappearance of the main transition at X(ch)=0.30 indicates that the pure L(o) phase can exist in X(ch)>0.30. The eutectic behavior observed in the phase diagram was explainable in terms of phase separation between two different types of regions with different types of regular distributions of cholesterol.
(キーワード): Calorimetry, Differential Scanning / Cholesterol / Crystallization / Lipid Bilayers / Phosphatidylcholines / Spectrometry, Fluorescence
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.colsurfb.2008.04.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18502622; ● Search Scopus @ Elsevier (PMID): 18502622; ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2008.04.004

(DOI: 10.1016/j.colsurfb.2008.04.004, PubMed: 18502622) 金品昌志, 後藤優樹, 多田佳織, 玉井伸岳, 松木均 :
エーテル結合型リン脂質における指組み構造形成,
高圧バイオサイエンスとバイオテクノロジー, Vol.2, No.1, 75-81, 2008年. 後藤優樹, 戸田雅隆, 玉井伸岳, 松木均, 金品昌志 :
高圧力下におけるジノナデカノイルホスファチジルコリン二分子膜の相挙動,
高圧バイオサイエンスとバイオテクノロジー, Vol.2, No.1, 68-74, 2008年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11229/hpbb.2.68
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.2.68

(DOI: 10.11229/hpbb.2.68) 松木均, 多田佳織, 後藤優樹, 玉井伸岳, 金品昌志 :
リン脂質膜の非二分子膜形成に対する熱力学的考察,
高圧バイオサイエンスとバイオテクノロジー, Vol.2, No.1, 61-67, 2008年.

(出版サイトへのリンク): ● Publication site (DOI): 10.11229/hpbb.2.61
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.11229/hpbb.2.61

(DOI: 10.11229/hpbb.2.61) Nobutake Tamai, Maiko Uemura, Tetsuya Takeichi, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
A new interpretation of eutectic behavior for distearoylphosphatidylcholine-cholesterol binary bilayer membrane,
Biophysical Chemistry, Vol.135, No.1-3, 95-101, 2008.

(要約): We investigated the thermotropic phase behavior of the distearoylphosphatidylcholine (DSPC)-cholesterol binary bilayer membrane as a function of the cholesterol composition (X(ch)) by fluorescence spectroscopy using 6-propionyl-2-(dimethylamino)naphthalene (Prodan) and differential scanning calorimetry (DSC). The fluorescence spectra, each of which has a single maximum, showed that the wavelength at the maximum intensity (lambda(max)) changed depending on the bilayer state: ca. 440 nm for the lamellar gel (L(beta)' or L(beta)) and the liquid ordered (L(o)) phases, ca. 470 nm for the ripple gel (P(beta)') phase and ca. 490 nm for the liquid crystalline (L(alpha)) phase, respectively. The transition temperatures were determined from the temperature dependences of the lambda(max) and endothermic peaks of the DSC thermograms. Both measurements showed that the pretransition disappears around X(ch)=0.035. The constructed temperature-X(ch) phase diagram indicated that the phase behavior of the binary bilayer membrane at X(ch)< or =0.15 is similar to that of general liquid-solid equilibrium for a binary system where both components are completely miscible in the liquid phase and completely immiscible in the solid phase. It was also revealed that the diagram has two characteristic points: a congruent melting point at X(ch)=0.08 and a peritectic-like point at X(ch)=0.15. The hexagonal lattice model was used for the interpretation of the phase behavior of the binary bilayer membrane. These characteristic compositions well correspond to the bilayer states in each of which cholesterol molecules are regularly distributed in the hexagonal lattice in a different way. That is, each composition of 0.035, 0.08 and 0.15 is nearly equal to that for the binary bilayer membrane which is entirely occupied with units, each composed of a cholesterol and 30 surrounding DSPC molecules within the next-next-next nearest neighbor sites (Unit (1:30): L(beta)(1:30)), with units, each of a cholesterol and 12 surrounding DSPC molecules within the next nearest sites (Unit (1:12): L(beta)(1:12)) or with units, each of a cholesterol and 6 surrounding DSPC molecules at the nearest neighbor sites (Unit (1:6): L(beta)(1:6)), respectively. Therefore, the eutectic behavior observed in the phase diagram was fully explainable in terms of a kind of phase separation between two different types of regions with different types of regular distributions of cholesterol. Further, the L(o) phase was found in the higher X(ch)-region (X(ch)>0.15). No endothermic peak over the temperature range from 10 to 80 degrees C at X(ch)=0.50 suggested that the single L(o) phase can exist at X(ch)>0.50.
(キーワード): Calorimetry, Differential Scanning / Cholesterol / Fluorescence / Fluorescent Dyes / Lipid Bilayers / Models, Biological / Phase Transition / Phosphatidylcholines / Thermodynamics / Transition Temperature
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bpc.2008.03.008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18436366; ● Search Scopus @ Elsevier (PMID): 18436366; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bpc.2008.03.008

(DOI: 10.1016/j.bpc.2008.03.008, PubMed: 18436366) Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Thermotropic and barotropic phase transitions of dilauroylphosphatidylcholine bilayer,
Chemistry and Physics of Lipids, Vol.153, No.2, 138-143, 2008.

(要約): The bilayer phase transitions of dilauroylphosphatidylcholine (DLPC), containing two linear acyl chains with 12 carbon atoms, were observed by means of differential scanning calorimetry (DSC) under ambient pressure and light transmittance under high pressure. When the heating scan for the DLPC bilayer in 50 wt.% aqueous ethylene glycol (EG) solution began at -30 degrees C after cold storage, the DSC thermogram showed two endothermic peaks at 1.7 and 4.5 degrees C, which correspond to the transition from the lamellar crystalline (Lc) phase to the intermediate liquid crystalline (Lx) phase and the transition from the Lx phase to the liquid crystalline (L) phase, respectively. Extremely large enthalpy change (32.9 kJ mol(-1)) is characteristic of the Lc/Lx phase transition. The DSC thermogram for the heating scan beginning from -10 degrees C showed a single endothermic peak with 9.2 kJ mol(-1) at -0.4 degrees C, which was assigned as the so-called main transition between the metastable ripple gel (P'(beta)) and metastable Lalpha phases. The DLPC bilayer under high pressure underwent three kinds of transitions in EG solution, whereas only one transition was observed in water under high pressure. The middle-temperature transition in EG solution could be assigned to the main transition because of its consistency with the main transition in water. The lower-temperature transition is probably assigned as transition from the Lc phase to the P'(beta) phase. Since the slope (dT/dp) of the Lc/P'(beta) phase boundary is smaller than that for the main transition, the Lc/P'(beta) phase boundary and the main transition curves crossed each other at 40 MPa on the temperature-pressure phase diagram. The higher-temperature transition in EG solution refers to the transition from the Lx phase to the Lalpha phase. The Lx phase disappeared at about 180 MPa, and the direct transition from the P'(beta) phase to the Lalpha phase was observed at high pressures above 180 MPa.
(キーワード): Calorimetry, Differential Scanning / Ethylene Glycol / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Pressure / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.chemphyslip.2008.03.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18394426; ● Search Scopus @ Elsevier (PMID): 18394426; ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2008.03.004

(DOI: 10.1016/j.chemphyslip.2008.03.004, PubMed: 18394426) Nobutake Tamai, Takayuki Matsui, Nobutaka Moribayashi, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Cholesterol Suppresses Pressure-induced Interdigitation of Dipalmitoylphosphatidylcholine Bilayer Membrane,
Chemistry Letters, Vol.37, No.6, 604-605, 2008.

(出版サイトへのリンク): ● Publication site (DOI): 10.1246/cl.2008.604
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1360283694087874304; ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2008.604

(DOI: 10.1246/cl.2008.604, CiNii: 1360283694087874304) Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Effect of hydrostatic pressure on the bilayer phase behavior of symmetric and asymmetric phospholipids with the same total chain length,
Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol.1778, No.4, 1067-1078, 2008.

(要約): The bilayer phase transitions of palmitoylstearoyl-phosphatidylcholine (PSPC), diheptadecanoyl-PC (C17PC) and stearoylpalmitoyl-PC (SPPC) which have the same total carbon numbers in the two acyl chains were observed by differential scanning calorimetry and high-pressure optical method. As the temperature increased, these bilayers exhibited four phases of the subgel (Lc), lamellar gel (L beta'), ripple gel (P beta') and liquid crystal (L alpha), in turn. The Lc phase was observed only in the first heating scan after cold storage. The temperatures of the phase transitions were almost linearly elevated by applying pressure. The temperature-pressure phase diagrams and the thermodynamic quantities associated with the phase transitions were compared among the lipid bilayers. For all the bilayers studied, the pressure-induced interdigitated gel (L beta I) phase appeared above the critical interdigitation pressure (CIP) between the L beta' and P beta' phases. The CIPs for the PSPC, C17PC and SPPC bilayers were found to be 50.6, 79.1 and 93.0 MPa, respectively. Contribution of two acyl chains to thermodynamic properties for the phase transitions of asymmetric PSPC and SPPC bilayers was not even. The sn-2 acyl chain lengths of asymmetric PCs governed primarily the bilayer properties. The fluorescence spectra of Prodan in lipid bilayers showed the emission maxima characteristic of bilayer phases, which were dependent on the location of Prodan in the bilayers. Second derivative of fluorescent spectrum exhibited the original emission spectrum of Prodan to be composed of the distribution of Prodan into multiple locations in the lipid bilayer. The F''497/F''430 value, a ratio of second derivative of fluorescence intensity at 497 nm to that at 430 nm, is decisive evidence whether bilayer interdigitation will occur. With respect to the L beta'/L beta I phase transition in the SPPC bilayer, the emission maximum of Prodan exhibited the narrow-range red-shift from 441 to 449 nm, indicating that the L beta I phase in the SPPC bilayer has a less polar "pocket" formed by a space between uneven terminal methyl ends of the sn-1 and sn-2 chains, in which the Prodan molecule remains stably.
(キーワード): 2-Naphthylamine / Calorimetry, Differential Scanning / Hydrostatic Pressure / Lipid Bilayers / Phase Transition / Phosphatidylcholines / Phospholipids / Spectrometry, Fluorescence / Temperature / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamem.2007.12.009
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18190778; ● Search Scopus @ Elsevier (PMID): 18190778; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamem.2007.12.009

(DOI: 10.1016/j.bbamem.2007.12.009, PubMed: 18190778) Masataka Kusube, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Bilayer Phase Transitions of N-methylated Dioleoylphosphatidylethanolamines under High Pressure,
Chemistry and Physics of Lipids, Vol.142, No.1-2, 94-102, 2006.

(要約): The bilayer phase transitions of four kinds of unsaturated phospholipids with different-sized polar head groups, dioleoylphosphatidylethanolamine (DOPE), dioleoylphosphatidyl-N-methylethanolamine (DOMePE), dioleoylphosphatidyl-N,N-dimethylethanolamine (DOMe2PE) and dioleoylphosphatidylcholine (DOPC), were observed by means of differential scanning calorimetry (DSC) and high-pressure light-transmittance. DSC thermogram and light-transmittance curve for each phospholipid vesicle solution exhibited only one phase transition under ambient pressure, respectively. The light-transmittance of DOPC solution at pressure higher than 234 MPa abruptly increased stepwise at two temperatures, which corresponds to the appearance of stable subgel and lamellar gel phases under high pressure in addition to the liquid crystal phase. The constructed temperature (T)-pressure (p) phase diagrams were compared among these phospholipids. The phase-transition temperatures of the phospholipids decreased stepwise by N-methylation of the head group. The slops of the T-p phase boundary (dT/dp) of DOPE, DOMePE and DOMe2PE bilayers (0.127-0.145 K MPa-1) were found to be close to that of the transition from the lamellar crystal (or subgel; Lc) phase to the liquid crystal (Lalpha) phase for DOPC bilayer (0.131 K MPa-1). On the other hand, the dT/dp value of the main transition from the lamellar gel (Lbeta) phase to the Lalpha phase for DOPC bilayer (0.233 K MPa-1) was significantly different from that of the Lc/Lalpha transition, hence both curves intersected with each other at 234 MPa. The thermodynamic quantities associated with the phase transition of DOPE, DOMePE and DOMe2PE bilayers had also similar values to those for the Lc/Lalpha transition of DOPC bilayer. Taking into account of the values of transition temperature, dT/dp and thermodynamic quantities compared with the corresponding results of saturated phospholipids, we identified the phase transitions observed in the DOPE, DOMePE and DOMe2PE bilayers as the transition from the Lc phase to the Lalpha phase although they have been regarded as the main transition in the previous studies. The Lbeta phase is probably unstable for DOPE, DOMePE and DOMe2PE bilayers at all pressures, it exists as a metastable phase at pressures below 234 MPa while as a stable phase at pressures above 234 MPa in DOPC bilayer. The difference in phase stability among the phospholipid bilayers is originated from that in hydration structure of the polar head groups.
(キーワード): Colorimetry / Lipid Bilayers / Methylation / Phase Transition / Phosphatidylcholines / Phosphatidylethanolamines / Stress, Mechanical / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.chemphyslip.2006.03.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16620796; ● Search Scopus @ Elsevier (PMID): 16620796; ● Search Scopus @ Elsevier (DOI): 10.1016/j.chemphyslip.2006.03.004

(DOI: 10.1016/j.chemphyslip.2006.03.004, PubMed: 16620796) Ryosuke Sueyoshi, Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Barotropic phase transition between the lamellar liquid crystal phase and the inverted hexagonal phase of dioleoylphosphatidylethanolamine,
Colloids and Surfaces B:Biointerfaces, Vol.50, No.1, 85-88, 2006.

(要約): The phase transition between the lamellar liquid crystal (Lalpha) phase and the inverted hexagonal (H(II)) phase of dioleoylphosphatidylethanolamine (DOPE) in aqueous NaCl solutions was observed by means of differential scanning calorimetry (DSC) under ambient pressure and light-transmittance technique under high pressure. The pressure dependence of the transition temperature (dT/dp) and the thermodynamic quantities for the Lalpha/H(II) transition were compared with those of another phase transition found in the DOPE bilayer membrane, which is the transition from the lamellar crystal (Lc) phase to the Lalpha phase. The dT/dp value of the Lalpha/H(II) transition was about 3.5 times as large as that of the Lc/Lalpha transition while the thermodynamic quantities were significantly smaller than those of the latter to the contrary. Comparing the enthalpy and volume behavior of the Lalpha/H(II) transition with that of the Lc/Lalpha transition, we concluded that the Lalpha/H(II) transition can be regarded as the volume-controlled transition for the reconstruction of molecular packing.
(キーワード): Calorimetry, Differential Scanning / Crystallization / Lipid Bilayers / Liquid Crystals / Models, Biological / Phase Transition / Phosphatidylethanolamines / Sodium Chloride / Solutions / Thermodynamics / Water
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.colsurfb.2006.04.001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16697154; ● Search Scopus @ Elsevier (PMID): 16697154; ● Search Scopus @ Elsevier (DOI): 10.1016/j.colsurfb.2006.04.001

(DOI: 10.1016/j.colsurfb.2006.04.001, PubMed: 16697154) Hitoshi Matsuki, Masaki GOTO, Masataka Kusube, Nobutake Tamai and Shoji Kaneshina :
Barotropic Phase Transitions of 1-Palmitoyl-2-stearoylphosphatidylcholine Bilayer Membrane,
Chemistry Letters, Vol.34, No.2, 270-271, 2005.

(要約): 非対称型リン脂質1-パルミトイル-2-ステアロイルホスファチジルコリン二分子膜の圧力誘起相転移を高圧光透過率測定および示差走査熱量測定により観測した．決定した相転移点に基づいて温度—圧力相図を構築し，3種類の対称型ホスファチジルコリン二分子膜の相図と比較した．1-パルミトイル-2-ステアロイルホスファチジルコリン二分子膜の相挙動は対称型ホスファチジルコリン二分子膜とは大きく異なり，指組み構造形成の臨界圧力は全ての脂質のうちで最も小さくなった．
(出版サイトへのリンク): ● Publication site (DOI): 10.1246/cl.2005.270
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1574231874639495424; ● Search Scopus @ Elsevier (DOI): 10.1246/cl.2005.270

(DOI: 10.1246/cl.2005.270, CiNii: 1574231874639495424)

MISC

後藤優樹 :
スイス連邦工科大学ポール・シェラー研究所留学記,
高圧力の科学と技術, Vol.25, No.3, 251-252, 2015年.

(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.25.251
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84941766485

(DOI: 10.4131/jshpreview.25.251, Elsevier: Scopus) 松木均, 後藤優樹, 玉井伸岳, 安澤幹人 :
人工生体膜のソフトナノテクノロジー,
徳島大学大学院ソシオテクノサイエンス研究部研究報告, Vol.55, 25-30, 2010年.

(要約): Soft nanotechnology is technology that treats soft and wet materials such as self-assemblysystems in a living body under a relatively moderate condition. We focus our attention on the sizecontrol of liposomes, namely lipid bilayer membranes and performed the soft nanotechnology insizing of liposomes under high pressure. Two high-pressure sizing techniques, a method ofcontinuous pressure relaxation using a phase transition between gel and liquid crystalline phases ofthe bilayer membrane and that of a pressure-induced gel phase using a phase transition betweenbilayer and nonbilayer membranes, were applied to liposomes of distearoylphosphatidylcholine, andthe effects of the both methods were considered. Further, the application of giant liposomescontrolled by high-pressure sizing to microsensor was briefly described.
(徳島大学機関リポジトリ): ● Metadata: 66693
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050001337463705088

(徳島大学機関リポジトリ: 66693, CiNii: 1050001337463705088)

総説・解説

後藤優樹 :
高圧力下における脂質ナノ粒子の相転移に関する研究,
膜, Vol.48, No.4, 163-167, 2023年7月.

(キーワード): 脂質二重膜 (lipid bilayer membrane) / リン脂質 / 高圧力 (high pressure)

後藤優樹 :
疎水鎖結合様式が脂質二重膜相挙動におよぼす影響:非天然アミド結合型リン脂質を用いた研究,
Colloid and Interface Communication, Vol.47, No.3, 18-21, 2022年8月. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
Membrane States of Saturated Glycerophospholipids: A Thermodynamic Study of Bilayer Phase Transitions,
Chemical & Pharmaceutical Bulletin, Vol.67, No.4, 300-307, Apr. 2019.

(キーワード): bilayer membrane / phase transition / glycerophospholipid / high pressure
(出版サイトへのリンク): ● Publication site (DOI): 10.1248/cpb.c18-00954
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30930432; ● Summary page in Scopus @ Elsevier: 2-s2.0-85064011553

(DOI: 10.1248/cpb.c18-00954, PubMed: 30930432, Elsevier: Scopus) 玉井伸岳, 後藤優樹, 松木均 :
圧力摂動熱量測定:脂質膜への適用,
熱測定, Vol.46, No.2, 76-80, 2019年4月.

(要約): Pressure perturbation calorimetry (PPC) is a novel calorimetric technique which allows us to obtain information about volumetric properties of solute molecules and colloidal particles in aqueous solutions. A PPC calorimeter has basically the same architecture as a high-sensitivity power-compensation differential scanning calorimeter, but it can measure the heat quantity transferred from or into a sample solution during the course of an isothermal small pressure change by attaching an external accessary for pressure control to the calorimeter. This heat quantity can be related to the expansion coefficient α, and thus, the temperature dependence of α can be obtained by performing PPC scans at a series of different temperatures. This article describes the application of PPC to the investigation of volume behavior of phospholipid bilayer membranes on the basis of our previous studies along with the general thermodynamic background of the PPC technique. We also give a concise explanation of how to analyze the PPC data, because we modified the conventional data analysis method to evaluate the absolute values of volume changes with bilayer phase transitions.
(キーワード): pressure perturbation calorimetry / lipid membrane / volume behavior / apparent molar volume / thermal expansivity
(出版サイトへのリンク): ● Publication site (DOI): 10.11311/jscta.46.2_76
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520290882709222784; ● Search Scopus @ Elsevier (DOI): 10.11311/jscta.46.2_76

(DOI: 10.11311/jscta.46.2_76, CiNii: 1520290882709222784) 松木均, 後藤優樹, 玉井伸岳 :
ホスファチジルエタノールアミン二重膜の温度および圧力誘起相転移,
膜, Vol.44, No.2, 40-49, 2019年3月.

(出版サイトへのリンク): ● Publication site (DOI): 10.5360/membrane.44.40
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.5360/membrane.44.40

(DOI: 10.5360/membrane.44.40) 後藤優樹, 玉井伸岳, 松木均 :
二本鎖イオン性界面活性剤の会合挙動:高圧力研究による膜状態の解明,
高圧力の科学と技術, Vol.28, No.2, 81-87, 2018年7月.

(要約): The bilayer phase transitions of double-chain cationic surfactants, dialkyldimethylammonium bromides (2CnBr), at ambient and high pressures were investigated. The 2CnBr bilayers underwent a single or multiple phase transitions depending on the alkyl-chain length. Comparing the phase-transition properties among the 2CnBr bilayers, the temperature pressure phase diagrams and thermodynamic quantities for the phase transitions were determined. We could comprehensively interpret the complicated phase behavior of the 2CnBr bilayers, which has not been revealed by conventional experimental methods at ambient pressure.
(キーワード): Double chain cationic surfactant / bilayer membrane / 相転移 (phase transition) / 高圧力 (high pressure)
(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.28.81
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85050766308

(DOI: 10.4131/jshpreview.28.81, Elsevier: Scopus) 玉井伸岳, 後藤優樹, 松木均 :
コレステロールが誘起する脂質膜側方相分離の熱力学的考察,
熱測定, Vol.44, No.3, 101-107, 2017年7月.

(出版サイトへのリンク): ● Publication site (DOI): 10.11311/jscta.44.3_101
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.11311/jscta.44.3_101

(DOI: 10.11311/jscta.44.3_101) 玉井伸岳, 後藤優樹, 松木均 :
圧力摂動熱量法によるリン脂質二分子膜の体積挙動および緩和挙動の定量的観測,
高圧力の科学と技術, Vol.25, No.2, 109-115, 2015年5月.

(要約): Pressure perturbation calorimetry (PPC) is a relatively new calorimetric technique which allows us to investigate volume behavior of solute molecules in aqueous solutions or colloidal particles in aqueous dispersions. This article deals with the application of the PPC technique to phospholipid vesicle dispersions along with a brief description about the theoretical aspect of this technique on the basis of our recent studies on the volume and relaxation behavior of phospholipid bilayers.
(キーワード): bilayer / isothermal compressibility / partial molar volume / phospholipid / pressure perturbation calorimetry / relaxation behavior / thermal expansivity
(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.25.109
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204380727040; ● Summary page in Scopus @ Elsevier: 2-s2.0-84931074285

(DOI: 10.4131/jshpreview.25.109, CiNii: 1390001204380727040, Elsevier: Scopus) 後藤優樹 :
ハロゲン化ジアルキルジメチルアンモニウム二重膜の会合挙動:高圧力研究による相状態の解明,
C & I commun colloid & interface communication : newsletter from DCSC, Vol.1, No.40, 31-33, 2015年3月. 玉井伸岳, 後藤優樹, 松木均 :
脂質ラフトは熱力学的に説明できるのか?―非調和融点型固液相図とクラスター形成―,
生物物理, Vol.54, No.3, 154-157, 2014年5月.

(出版サイトへのリンク): ● Publication site (DOI): 10.2142/biophys.54.154
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2142/biophys.54.154

(DOI: 10.2142/biophys.54.154) 松木均, 後藤優樹, 玉井伸岳 :
飽和ジアシルホスファチジルコリン二重膜の温度および圧力誘起相転移,
熱測定, Vol.41, No.2, 66-73, 2014年4月.

(出版サイトへのリンク): ● Publication site (DOI): 10.11311/jscta.41.2_66
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.11311/jscta.41.2_66

(DOI: 10.11311/jscta.41.2_66) 後藤優樹 :
高圧蛍光法によるホスファチジルコリン二重膜のイメージング,
高圧力の科学と技術, Vol.23, No.1, 157-166, 2013年5月.

(要約): High-pressure fluorescence study using a polarity-sensitive probe Prodan on phosphatidylcholine (PC) bilayers is reviewed. The author has constructed a three-dimensional image plot based on the second-derivative of the Prodan fluorescence spectra that change depending on the phase state of the PC bilayers, which enables us to examine the packing state of the bilayer. The effects of (1) acyl chain length, (2) vesicle size, and (3) chain asymmetry on the PC bilayers are discussed by using the technique of bilayer imaging. Further the formation of the lamellar crystal phase of the PC bilayer is also revealed by the technique.
(キーワード): Bilayer membrane / Bilayer imaging / Packing state / Phosphatidylcholine / Phase transition / Prodan fluorescence
(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.23.157
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679358404224; ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.23.157

(DOI: 10.4131/jshpreview.23.157, CiNii: 1390282679358404224) 松木均, 後藤優樹 :
高圧力による脂質の相転移制御,
冷凍, Vol.88, No.1026, 274-279, 2013年4月.

(キーワード): 高圧力 / 脂質二重膜 / 相安定性 / 相転移 / 中性脂肪 / 非二重膜構造
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520009408125051776

(CiNii: 1520009408125051776) 松木均, 後藤優樹, 玉井伸岳 :
生体膜脂質の膜状態 -圧力研究から見えてくる構造機能相関-,
高圧力の科学と技術, Vol.23, No.1, 30-38, 2013年2月.

(要約): Bilayer-membrane properties of the most frequently used lipid in membrane studies, dipalmitoylphosphatidylcholine (DPPC), are described. And then, barotropic bilayer phase behavior of various kinds of phospholipids with a different molecular structure from DPPC is explained in comparison with that of the DPPC bilayer. A slight difference in the molecular structure has a marked influence on the membrane state of the bilayer. Systematic pressure study using the DPPC bilayer enables us to understand the role and meaning of various phospholipids existing in biological membranes.
(キーワード): bilayer membrane / dipalmitoylphosphatidylcholine / phase stability / phase transition / phospholipids / pressure
(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.23.30
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204380540672; ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.23.30

(DOI: 10.4131/jshpreview.23.30, CiNii: 1390001204380540672) Hitoshi Matsuki, Masaki GOTO, Kaori Tada and Nobutake Tamai :
Thermotropic and Barotropic Phase Behavior of Phosphatidylcholine Bilayers, --- Phospholipids: Molecular Sciences 2012 ---,
International Journal of Molecular Sciences, Vol.14, No.2, 2282-2302, Jan. 2013.

(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms14022282
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84875095682

(DOI: 10.3390/ijms14022282, Elsevier: Scopus) 松木均, 多田佳織, 後藤優樹, 玉井伸岳 :
非対称リン脂質二重膜の高圧相転移:分子非対称性と相安定性,
高圧力の科学と技術, Vol.20, No.4, 306-314, 2010年11月.

(要約): The effect of pressure on the bilayer phase transitions of two kinds of asymmetric phospholipids, phosphatidylcholines (PCs) with different saturated acyl chains in the sn-1 and sn-2 positions on the glycerol backbone and mixed-chain PCs with an unsaturated acyl chain in the sn-1 or sn-2 position, is described. The alignment of the sn-1 and sn-2 acyl chains at the therminal methyl ends and the mismatch of the effective chain length between the sn-1 and sn-2 acyl chains produce a significant influence on their bilayer properties. The correlation between molecular asymmetry of PC molecules and phase stabilities of the bilayer membranes is discussed.
(キーワード): asymmetric phospholipid / bilayer membrane / phase stability / phase transition / pressure / symmetric phospholipid
(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.20.306
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204381315968; ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.20.306

(DOI: 10.4131/jshpreview.20.306, CiNii: 1390001204381315968) 後藤優樹 :
高圧蛍光法による対称および非対称型リン脂質二重膜相挙動の研究,
高圧力の科学と技術, Vol.18, No.3, 267-272, 2008年8月.

(要約): High-pressure fluorescence study on the bilayer phase behavior of symmetric and asymmetric phospholipids in my doctoral thesis is briefly reviewed. It has been clarified by the novel analysis of the second derivatives of fluorescent spectra that the fluorescent probe Prodan resides multiple sites in the bilayer membrane of phosphatidylcholines (PCs) depending on the membrane states. Comparing the diagrams of three dimensional fluorescent spectra among them, the present results revealed that the slight difference in acyl chain length between the sn-1 and sn-2 positions in the PC molecules provides significant influence on their bilayer phase behavior, especially in the bilayer interdigitation.
(キーワード): asymmetric phospholipid / bilayer membrane / phase transition / fluorescence / pressures-induced interdigitation / prodan fluorescence
(出版サイトへのリンク): ● Publication site (DOI): 10.4131/jshpreview.18.267
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001204381120000; ● Search Scopus @ Elsevier (DOI): 10.4131/jshpreview.18.267

(DOI: 10.4131/jshpreview.18.267, CiNii: 1390001204381120000)

講演・発表

Kana Kajiura, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Phase Transitions of Acidic Phospholipid Bilayer Membranes: Effect of Charged Head-Group Size,
26th IUPAC International Conference on Chemical Thermodynamics (ICCT-2023), Osaka, Aug. 2023. Nobutake Tamai, Mei Kamiya, Masaki GOTO and Hitoshi Matsuki :
Bilayer Phase Transitions of Phospholipid in Aqueous Solutions of Monosaccharides,
26th IUPAC International Conference on Chemical Thermodynamics (ICCT-2023), Osaka, Aug. 2023. Masaki GOTO, Ayaka Mukae, Shuntaro Yoshida, Nobutake Tamai and Hitoshi Matsuki :
Pressure-Induced Bilayer Interdigitation of Lipid Membranes: Characteristics and Formation Mechanism,
26th IUPAC International Conference on Chemical Thermodynamics (ICCT-2023), Osaka, Aug. 2023. Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki :
Bilayer Phase Imaging by Prodan Fluorescence Spectroscopy,
13th International Congress on Membranes and Membrane Processes (ICOM2023), Chiba, Jul. 2023. Masaki GOTO, Ayaka Mukae, Nobutake Tamai and Hitoshi Matsuki :
Pressure-Induced Interdigitation of Lipid Membranes: Difference from Chemically Induced Interdigitation and the Formation Mechanism,
13th International Congress on Membranes and Membrane Processes (ICOM2023), Chiba, Jul. 2023. Nobutake Tamai, Nono Kiriyama, Masaki GOTO, Hitoshi Matsuki and Kazuhiro Fukada :
Thermodynamic Study on the Effect of Monosaccharides on Phase Transitions of Phospholipid Bilayer Membrane,
The 8th International Symposium of International Society of Rare Sugars (RSC2023), Takamatsu, Apr. 2023. Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Thermodynamic Study on Bilayer Phase Transitions of Twin-Tailed Cationic Surfactants,
The 5th International Kyushu Colloid Colloquium, Okinawa, Nov. 2019. Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
High-Pressure Fluorometric Study on Bilayer Packing of Phosphatidylcholines,
Okinawa Colloid 2019, Okinawa, Nov. 2019. Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki :
Suppression of the Formation of Intgerdigitated Structure in Ether-Linked Phosphatidylcholine Bilayer by Cholesterol,
Okinawa Colloid 2019, Okinawa, Nov. 2019. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
Membrane Fusion of Phospholipid Bilayers Induced by High Pressure,
Okinawa Colloid 2019, Okinawa, Nov. 2019. Masaki GOTO, Shuntaro Yoshida, Nobutake Tamai and Hitoshi Matsuki :
Thermodynamic Study on Ligand Partitioning into Lipid Bilayer Membranes under High Pressure,
10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018), Numazu, Sep. 2018. Shuntaro Yoshida, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Prodan Is a Useful Packing Indicator in Lipid Membranes, Which Discerns Various Kinds of Phase Transitions,
10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018), Numazu, Sep. 2018. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
Pressure-Induced Membrane Fusion of Phospholipid Bilayers: Irreversible and Spherical Growth of Giant Unilamellar Vesicles,
10th International Conference on High Pressure Bioscience and Biotechnology (HPBB2018), Numazu, Sep. 2018. Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki :
Thermotropic Phase Behavior of Sterol-Containing Binary Bilayer Membranes of Diacylphosphatidylcholines,
8th International and 10th Japan-China Joint Symposium on Calorimetry and Thermal Analysis (CATS-2017), Fukuoka, Nov. 2017. Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki :
Pressure Perturbation Calorimetry for the Volume Characterization of Lipid Bilayers,
4th Joint Meeting of the 71st Calorimetry Conference (CALCON2016) and the Japan Society of Calorimetry and Thermal Analysis (JSCTA), Turtle Bay, Oahu, Hawaii, Aug. 2016. Hitoshi Matsuki, Shigeru Endo, Ryosuke Sueyoshi, Masaki GOTO and Nobutake Tamai :
Temperature- and Pressure-Induced Phase Transitions of Diacylphosphatidylethanolamine Bilayers,
4th Joint Meeting of the 71st Calorimetry Conference (CALCON2016) and the Japan Society of Calorimetry and Thermal Analysis (JSCTA), Turtle Bay, Oahu, Hawaii, Aug. 2016. Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Effect of Pressure on Bilayer Phase Behavior of N-methylated Di-O-hexadecylphosphatidylethanolamines,
9th International Conference on High Pressure Bioscience and Biotechnology (HPBB2016), Toronto, Jul. 2016. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
Effect of Hydrophobic Chain Structure on Phase Transitions of Diacylphosphatidylethanolamine Bilayers under High Pressure,
9th International Conference on High Pressure Bioscience and Biotechnology (HPBB2016), Toronto, Jul. 2016. Takahiro Yano, Yoshioka Tatsuro, Nobutake Tamai, Masaki GOTO, Hitoshi Matsuki, Nagasawa Makoto and Matsumoto Masamitsu :
Precise Measuring System of Density for Liquids under High Pressure,
8th International Conference on High Pressure Bioscience and Biotechnology (HPBB2014), Nantes, France, Jul. 2014. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
Barotropic Structural Change of Lipid Membranes: Characteristics and Mechanism of Bilayer Interdigitation,
8th International Conference on High Pressure Bioscience and Biotechnology (HPBB2014), Nantes, France, Jul. 2014. Takahiro Yano, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Barotropic Phase Behavior of Asymmetric Phosphatidylcholine Bilayers,
8th International Conference on High Pressure Bioscience and Biotechnology (HPBB2014), Nantes, France, Jul. 2014. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
Pressure-induced Bilayer Interdigitation: Model of Molecular-ordering Formation in Lipid Membranes,
The 2nd International Symposium ``Dynamical ordering of biomolecular systems for creation of integrated functions'', Kyoto, Jan. 2014. Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
High-pressure fluorometric study on the subgel-formation of phosphatidylcholine bilayer membrane,
7th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2012), Otsu, Nov. 2012. Nobutake Tamai, Masaki GOTO and Hitoshi Matsuki :
An attempt to determine the viscoelastic properties of phospholipid bilayer membranes,
7th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2012), Otsu, Nov. 2012. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
How do membranes response by applying high hydrostatic pressure?,
7th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2012), Otsu, Nov. 2012. Nobutake Tamai, Sanae Inazawa, Daiki Fijiwara, Masaki GOTO, Shoji Kaneshina and Hitoshi Matsuki :
Effect of Stigmasterol and Ergosterol on the Thermotropic Bilayer Phase Behavior of Dipalmitoylphosphatidylcholine,
14th International Association of Colloid and Interface Scientists, Conference (IACIS2012), Sendai, May 2012. Hitoshi Matsuki, Saeko Tanaka, Masaki GOTO and Nobutake Tamai :
Barotropic Bilayer Phase Behavior of an Acidic Phospholipid, Dipalmitoylphosphatidylglycerol,
14th International Association of Colloid and Interface Scientists, Conference (IACIS2012), Sendai, May 2012. Saeko Tanaka, Masaki GOTO, Nobutake Tamai and Hitoshi Matsuki :
Phase Behavior of Dipalmitoylphosphatidylglycerol Bilayer Membrane in Saline Water under High Pressure,
6th International Conference on Advanced Materials Development and Performance (AMDP2011), Tokushima, Jul. 2011. Nobutake Tamai, Maiko Tsutsui, Masaki GOTO and Hitoshi Matsuki :
Investigation on Volumetric Properties of Phospholipid Aggregates by Pressure Perturbation Calorimetry,
6th International Conference on Advanced Materials Development and Performance (AMDP2011), Tokushima, Jul. 2011. Hitoshi Matsuki, Masaki GOTO and Nobutake Tamai :
High-pressure fluorescence study on packing states of phosphatidylcholine bilayers,
The 6th meeting of the Study of Matter at Extreme Conditions (SMEC2011), Miami, Apr. 2011. Sanae Inazawa, Takuya Izumikawa, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Comparison between effects of cholesterol and stigmasterol on bilayer phase behavior of dipalmitoylphosphatidylcholine,
The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Dec. 2010. Maiko Tsutsui, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Themotropic volume behavior of bilayer membranes of saturated and unsaturated phosphatidylcholines,
The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Dec. 2010. Nobutake Tamai, Takuya Izumikawa, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Thermotropic phase behavior of binary bilayer membranes of cholesterol and a homologous series of saturated diasylphosphatidylcholines of different chain lengths,
The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Dec. 2010. Hitoshi Matsuki, Agnieszka Broniek, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Phase behavior of plasmalogen bilayer membrane under high pressure,
The 3rd International Kyushu Colloid Colloquium, Fukuoka, Sep. 2010. Hitoshi Matsuki, Hiroshi Sawaguchi, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Subgel Phase Formation on Bilayer Membranes of Diacyl- and Dialkylphosphatidylcholines with Varying Acyl Chain Lengths,
International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010. Saeko Tanaka, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Phase Behavior of Dipalmitoylphosphatidylglycerol Bilayer under High Pressure,
International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010. Ryo Kishizoe, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Anomalous Phase Behavior of Ditridecanoylphosphatidylcholine Bilayer under High Pressure,
International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010. Sanae Inazawa, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Comparative Study on Effects of Stigmasterol and Cholesterol on Bilayer Phase Behavior of Dipalmitoylphosphatidylcholine,
International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010. Nobutake Tamai, Takuya Izumikawa, Maiko Uemura, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Acyl-chain Length Dependent and Independent Effects of Chlolesterol on Bilayer Phase Behavior of Diacylphosphatidylcholines,
International Conference on Nanoscopic Colloid and Surface Science (NCSS2010), Chiba, Sep. 2010. Nobutake Tamai, Yuko Nambu, Maiko Tsutsui, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
Volume properties of diacylphosphatidylcholine bilayer membranes revealed by pressure perturbation calorimetry and densitometry,
6th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2010), Freising, Germany, Aug. 2010. Hitoshi Matsuki, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Packing state in bilayer membranes of diacylphosphatidylcholines with varying acyl chain lengths under high pressure,
6th Internationl Conference on High Pressure Bioscience and Biotechnology (HPBB2010), Freising, Germany, Aug. 2010. Nobutake Tamai, Masaki GOTO, Hitoshi Matsuki and Shoji Kaneshina :
A mechanism of pressure-induced interdigitation of lipid bilayers,
Journal of Physics: Conference Series, Vol.215, No.1, 12161, Bristol, Mar. 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1088/1742-6596/215/1/012161
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012161

(DOI: 10.1088/1742-6596/215/1/012161) Takuya Izumikawa, Yuko Nambu, Kazuyo Fujishige, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Volume behaviour of dipalmitoylphosphatidylcholine bilayer membrane: pressure perturbation calorimetry and densitometry,
Journal of Physics: Conference Series, Vol.215, No.1, 12163, Bristol, Mar. 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1088/1742-6596/215/1/012163
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012163

(DOI: 10.1088/1742-6596/215/1/012163) Kaori Tada, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Effect of pressure on the bilayer phase transitions of asymmetric lipids with an unsaturated acyl chain in sn-1 position,
Journal of Physics: Conference Series, Vol.215, No.1, 12162, Bristol, Mar. 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1088/1742-6596/215/1/012162
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012162

(DOI: 10.1088/1742-6596/215/1/012162) Makoto Nishimoto, Masaki GOTO, Nobutake Tamai, Hideaki Nagamune, Shoji Kaneshina and Hitoshi Matsuki :
Effect of pressure on interactions of anti-fluorescent probe monoclonal antibody with a ligand and inhibitors,
Journal of Physics: Conference Series, Vol.215, No.1, 12157, Bristol, Mar. 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1088/1742-6596/215/1/012157
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012157

(DOI: 10.1088/1742-6596/215/1/012157) Hitoshi Matsuki, Makoto Nishimoto, Kaori Tada, Masaki GOTO, Nobutake Tamai and Shoji Kaneshina :
Thermodynamic characterization of bilayer-nonbilayer phase transitions of phospholipid membranes,
Journal of Physics: Conference Series, Vol.215, No.1, 12160, Bristol, Mar. 2010.

(出版サイトへのリンク): ● Publication site (DOI): 10.1088/1742-6596/215/1/012160
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1088/1742-6596/215/1/012160

(DOI: 10.1088/1742-6596/215/1/012160) Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Bilayer Phase Behavior of an Asymmetric Lipid, 1-Stearoyl-2-palmitoylphosphatidylcholine, under High Pressure,
The Proceedings of 4th Internationl Conference on High Pressure Bioscience and Biotechnology, Vol.1, 73-78, Tsukuba, Aug. 2007. Kaori Tada, Masaki GOTO, Masataka Kusube, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Barotropic Phase Transitions of Dilauroylphosphatidylcholine Bilayer Membrane,
The Proceedings of 4th Internationl Conference on High Pressure Bioscience and Biotechnology, Vol.1, 68-72, Tsukuba, Aug. 2007. Masataka Kusube, Masaki GOTO, Nobutake Tamai, Hitoshi Matsuki and Shoji Kaneshina :
Effect of Pressure on the Prodan Fluorescence in Bilayer Membrane of Ether-linked Lipid, Dihexadecylphosphatidylcholine,
The Proceedings of 4th Internationl Conference on High Pressure Bioscience and Biotechnology, Vol.1, 61-67, Tsukuba, Aug. 2007. 後藤優樹, 松下里李夏, 玉井伸岳, 松木均 :
リン脂質二重膜は光学活性リガンドを認識できるのか?,
2023年度麻酔メカニズム研究会, 2023年12月. 角田芙美, 玉井伸岳, 後藤優樹, 安澤幹人, 松木均 :
極性頭部荷電が異なるコリンホスフェート型脂質の有機合成,
2023年度日本化学会中国四国支部大会, 2023年11月. 角田芙美, 玉井伸岳, 後藤優樹, 安澤幹人, 松木均 :
荷電状態の異なる極性頭部転置型リン脂質の有機合成,
第37回九州コロイドコロキウム, 2023年11月. 中尾俊樹, 後藤優樹, 松木均, 加藤稔 :
FTIR法によるラセミ体アミド結合型リン脂質の温度・圧力誘起相転移,
第64回高圧討論会, 2023年11月. 榎本賢太郎, 尾形茉実, 後藤優樹, 玉井伸岳, 松木均 :
高圧力下におけるハロゲン化ジアルキルジメチルアンモニウム二重膜の相挙動,
第64回高圧討論会, 2023年11月. 羽原誉幸, 川原宏介, 後藤優樹, 玉井伸岳, 松木均 :
不飽和リン脂質混合二重膜の圧力誘起膜融合,
第64回高圧討論会, 2023年11月. 松岡颯大, 梶浦可菜, 後藤優樹, 玉井伸岳, 松木均 :
高圧下における酸性リン脂質ジパルミトイルホスファチジン酸二重膜の相挙動,
第64回高圧討論会, 2023年11月. 後藤優樹, 松岡楓太, 玉井伸岳, 松木均 :
酸性リン脂質ジミリストイルホスファチジルグリセロール二重膜の圧力誘起相転移,
第64回高圧討論会, 2023年11月. 松木均, 後藤優樹, 玉井伸岳 :
脂質膜研究の新展開:系統脂質学から化学脂質学へ,
第64回高圧討論会, 2023年11月. 玉井伸岳, 桐山野乃, 神谷芽生, 後藤優樹, 深田和宏, 松木均 :
化学構造の異なる四種類のアルドおよびケトヘキソースがリン脂質二重膜の相転移におよぼす影響,
第59回熱測定討論会, 2023年10月. 梶浦可菜, 松岡颯大, 後藤優樹, 玉井伸岳, 松木均 :
極性頭部の有無が酸性リン脂質の二重膜相転移におよぼす影響:特異的な添加塩濃度依存性,
第74回コロイドおよび界面化学討論会, 2023年9月. 後藤優樹, 玉井伸岳, 松木均 :
ジアルキルホスファチジルコリン二重膜のサブゲル相形成,
第74回コロイドおよび界面化学討論会, 2023年9月. 角田芙美, 後藤優樹, 玉井伸岳, 松木均 :
配列変異非天然脂質の有機合成,
第14回日本生物物理学会中国四国支部大会, 2023年5月. 玉井伸岳, 角田芙美, 後藤優樹, 松木均 :
系統脂質学から化学脂質学へ:新たな脂質膜研究の展開,
第14回日本生物物理学会中国四国支部大会, 2023年5月. 後藤優樹, 中尾俊樹, 玉井伸岳, 松木均 :
ラセミ体アミド結合型長鎖ホスファチジルコリンの圧力誘起二重膜相転移,
第63回高圧討論会, 2022年12月. 梶浦可菜, 松岡颯大, 後藤優樹, 玉井伸岳, 松木均 :
リン脂質二重膜の圧力誘起相転移 -酸性リン脂質ジパルミトイルホスファチジン酸-,
第63回高圧討論会, 2022年12月. 関谷朋美, 後藤優樹, 玉井伸岳, 松木均 :
光散乱法によるリン脂質ベシクルの形態観察,
2022年度日本化学会中国四国支部大会, 2022年11月. 山崎名津美, 後藤優樹, 玉井伸岳, 松木均 :
極性頭部転置ベタイン型脂質の有機合成と二重膜物性,
2022年度日本化学会中国四国支部大会, 2022年11月. 玉井伸岳, 横矢祐香, 後藤優樹, 松木均 :
アルキル鎖長の異なる1:1-カチオンーアニオン界面活性剤イオン対の水中における会合体形成,
第58回熱測定討論会, 2022年10月. Toshiki Nakao, Masaki GOTO, Nobutake Tamai, Minoru Kato and Hitoshi Matsuki :
Bilayer Properties of a Glycero- and Sphingo-Mixed Type Phospholipid,
The 73rd Divisional Meeting of Division of Colloid and Surface Chemistry, The Chemical Society of Japan, Sep. 2022. 玉井伸岳, 横矢祐香, 後藤優樹, 松木均 :
水中での非対称型カチオン-アニオン界面活性剤の会合体形成,
第73回コロイドおよび界面化学討論会, 2022年9月. 後藤優樹, 中尾俊樹, 玉井伸岳, 松木均 :
高圧下におけるラセミ体アミド結合型長鎖ホスファチジルコリン二重膜の相挙動,
第73回コロイドおよび界面化学討論会, 2022年9月. 桐山野乃, 神谷芽生, 玉井伸岳, 後藤優樹, 深田和宏, 松木均 :
単糖水溶液中でのリン脂質二分子膜のゲル-液晶相転移,
第36回九州コロイドコロキウム, 2022年8月. 松岡楓太, 後藤優樹, 玉井伸岳, 松木均 :
高圧下におけるジミリストイルホスファチジルグリセロール二重膜の相挙動,
第36回九州コロイドコロキウム, 2022年8月. 梶浦可菜, 成瀬由希, 後藤優樹, 玉井伸岳, 松木均 :
酸性リン脂質ホスファチジン酸の二重膜相転移:荷電頭部サイズの効果,
第36回九州コロイドコロキウム, 2022年8月. 梶浦可菜, 成瀬由希, 後藤優樹, 玉井伸岳, 松木均 :
酸性リン脂質二重膜の相転移:荷電頭部サイズが膜状態におよぼす影響,
日本膜学会第44年会, 2022年6月. 桐山野乃, 神谷芽生, 玉井伸岳, 後藤優樹, 深田和宏, 松木均 :
リン脂質二重膜のゲル–液晶相転移におよぼす単糖の効果,
日本膜学会第44年会, 2022年6月. 松岡楓太, 後藤優樹, 玉井伸岳, 松木均 :
ジミリストイルホスファチジルグリセロール二重膜の圧力誘起相転移,
第13回日本生物物理学会中国四国支部大会, 2022年5月. 名田智哉, 尾形茉実, 後藤優樹, 玉井伸岳, 松木均 :
ヨウ化ジテトラデシルジメチルアンモニウム二重膜の相挙動,
第13回日本生物物理学会中国四国支部大会, 2022年5月. 迎綾香, 下田毬絵, 金岡大樹, 新居佳孝, 山﨑博子, 湯浅恵造, 後藤優樹, 玉井伸岳, 松木均 :
柑橘類果皮抽出液による真皮線維芽細胞コラーゲン産生作用,
第13回日本生物物理学会中国四国支部大会, 2022年5月. 大西沙弥, 西谷真美, 下野萌香, 下田毬絵, 金岡大樹, 山﨑博子, 湯浅恵造, 後藤優樹, 玉井伸岳, 松木均 :
スダチ果皮抽出液による表皮アクアポリン3の発現制御機構の解明,
第13回日本生物物理学会中国四国支部大会, 2022年5月. 角田芙美, 中尾俊樹, 後藤優樹, 玉井伸岳, 松木均 :
光学活性アミド結合型リン脂質の有機合成と二重膜特性,
第13回日本生物物理学会中国四国支部大会, 2022年5月. 中尾俊樹, 後藤優樹, 倉科昌, 玉井伸岳, 安澤幹人, 松木均 :
ホスファチジルコリン二重膜におよぼす疎水鎖結合様式の影響,
第59回生物物理学会年会, 2021年11月. 松木均, 後藤優樹, 玉井伸岳 :
エーテル結合型ホスファチジルコリン膜のサブゲル相形成,
膜シンポジウム2021, 2021年11月. 玉井伸岳, 清水建翔, 後藤優樹, 松木均 :
アルキル鎖長の等しい1:1-カチオン-アニオン界面活性剤イオン対の水中における会合体形成,
第57回熱測定討論会, 2021年10月. 後藤優樹, 中尾俊樹, 玉井伸岳, 松木均 :
高圧下におけるアミド結合型リン脂質ジアシルアミドデオキシホスファチジルコリン二重膜の相挙動,
第62回高圧討論会, 2021年10月. 松木均, 後藤優樹, 玉井伸岳 :
ホスファチジルコリン二重膜のサブゲル相形成,
第62回高圧討論会, 2021年10月. 松下里李夏, 後藤優樹, 玉井伸岳, 松木均 :
光学活性リン脂質二重膜の相転移とリガンド認識,
第35回九州コロイドコロキウム, 2021年9月. 中尾俊樹, 後藤優樹, 倉科昌, 玉井伸岳, 安澤幹人, 松木均 :
グリセロ-スフィンゴ混合型非天然リン脂質の二分子膜相転移,
第35回九州コロイドコロキウム, 2021年9月. 後藤優樹, 中尾俊樹, 玉井伸岳, 松木均 :
アミド結合型リン脂質ジアシルアミドデオキシホスファチジルコリン二重膜の常圧および高圧相転移,
第72回コロイドおよび界面化学討論会, 2021年9月. 玉井伸岳, 清水建翔, 後藤優樹, 松木均 :
水中での対称型カチオン-アニオン界面活性剤の会合体形成,
第72回コロイドおよび界面化学討論会, 2021年9月. 長尾苑, 玉井伸岳, 後藤優樹, 松木均 :
光散乱法によるリン脂質ベシクルの構造安定性評価,
日本膜学会第43年会, 2021年6月. 中尾俊樹, 後藤優樹, 倉科昌, 玉井伸岳, 安澤幹人, 松木均 :
モジュール構造変更アナログ脂質の合成と二重膜物性,
日本膜学会第43年会, 2021年6月. Toshiki Nakao, Masaki GOTO, Masashi Kurashina, Nobutake Tamai, Mikito Yasuzawa and Hitoshi Matsuki :
Organic Synthesis and Bilayer Properties of a Sphingolipid Analog, an Amide-Linked Phosphatidylcholine,
12th Annual Meeting of Chugoku/Shikoku Branch in the Biophysical Society of Japan, May 2021. 荒井祐徹, 今村比呂志, 後藤優樹, 玉井伸岳, 松木均, 加藤稔 :
ジミリストイルホスファチジルコリン二重膜の温度・圧力相転移に関するFTIR研究,
第61回高圧討論会, 2020年12月. 後藤優樹, 玉井伸岳, 松木均 :
飽和ジアルキルホスファチジルコリン二重膜の熱力学的相図,
第61回高圧討論会, 2020年12月. 長安真舜, 川原宏介, 後藤優樹, 玉井伸岳, 松木均 :
高圧力が誘起する不飽和リン脂質混合二重膜の膜融合とその分子メカニズム,
2020年度日本化学会中国四国支部大会, 2020年11月. 神谷芽生, 後藤優樹, 玉井伸岳, 松木均 :
非対称型カチオン-アニオン界面活性剤の水中での会合体形成,
2020年度日本化学会中国四国支部大会, 2020年11月. 尾形茉実, 本橋牧子, 青木雄椰, 後藤優樹, 玉井伸岳, 松木均 :
ハロゲン化ジドデシルジメチルアンモニウム二重膜相挙動におよぼす対イオン効果,
2020年度日本化学会中国四国支部大会, 2020年11月. 玉井伸岳, 清水建翔, 横矢祐香, 後藤優樹, 松木均 :
対称型カチオン–アニオン界面活性剤塩の水中での会合体形成に関する熱力学的研究,
膜シンポジウム2020, 2020年11月. 中尾俊樹, 後藤優樹, 倉科昌, 玉井伸岳, 安澤幹人, 松木均 :
スフィンゴリン脂質類似アミド型リン脂質二重膜の熱的相転移:鎖結合様式の対照的効果,
第56回熱測定討論会, 2020年10月. 中尾俊樹, 後藤優樹, 倉科昌, 玉井伸岳, 安澤幹人, 松木均 :
スフィンゴ脂質類似アミド結合型ホスファチジルコリンの有機合成と二重膜物性,
日本膜学会第42年会, 2020年6月. 川原宏介, 後藤優樹, 玉井伸岳, 松木均 :
高圧力下における不飽和リン脂質混合二重膜ベシクルの融合条件,
2019年度日本化学会中国四国支部大会, 2019年11月. 中尾俊樹, 倉科昌, 後藤優樹, 玉井伸岳, 安澤幹人, 松木均 :
アミド結合型ホスファチジルコリンの有機合成および二分子膜相転移評価,
2019年度日本化学会中国四国支部大会, 2019年11月. 野村拓弘, 成瀬由希, 岡本裕嗣, 後藤優樹, 玉井伸岳, 松木均 :
高圧蛍光法によるジパルミトイルホスファチジルグリセロール二重膜内充填構造の評価,
2019年度日本化学会中国四国支部大会, 2019年11月. 田中杏奈, 本橋牧子, 青木雄椰, 後藤優樹, 玉井伸岳, 松木均 :
塩化ジアルキルジメチルアンモニウムの合成と二重膜相挙動の解明,
2019年度日本化学会中国四国支部大会, 2019年11月. 吉田俊太郎, 後藤優樹, 玉井伸岳, 松木均 :
高圧力下におけるDPPC二重膜のエタノール誘起指組み構造形成,
2019年度日本化学会中国四国支部大会, 2019年11月. 原山侑也, 後藤優樹, 玉井伸岳, 松木均 :
荷電状態の異なる極性頭部転置型リン脂質二分子膜の合成と膜物性,
2019年度日本化学会中国四国支部大会, 2019年11月. 木口碧, 後藤優樹, 玉井伸岳, 松木均 :
中鎖ホスファチジルコリン二重膜のゲル-液晶中間相状態,
2019年度日本化学会中国四国支部大会, 2019年11月. 平井悠貴, 玉井伸岳, 後藤優樹, 松木均 :
ハロゲン化ジアルキルジメチルアンモニウム二分子膜の体積挙動,
2019年度日本化学会中国四国支部大会, 2019年11月. 清水建翔, 玉井伸岳, 後藤優樹, 松木均 :
水中における対称型カチオン-アニオン界面活性剤の会合体形成,
2019年度日本化学会中国四国支部大会, 2019年11月. 玉井伸岳, 稲澤早苗, 後藤優樹, 松木均 :
植物および菌類由来のステロールが誘起するリン脂質二分子膜側方相分離挙動,
膜シンポジウム2019, 2019年11月. 松木均, 木口碧, 中尾俊樹, 本橋牧子, 後藤優樹, 玉井伸岳 :
中鎖ホスファチジルコリン二重膜のゲル-液晶中間状態:出現様式と形成機構,
膜シンポジウム2019, 2019年11月. 後藤優樹, 岡本裕嗣, 成瀬由希, 野村拓弘, 玉井伸岳, 松木均 :
ジステアロイルホスファチジルコリン二重膜の高圧相挙動,
第60回高圧討論会, 2019年10月. 成瀬由希, 中江ひかる, 山口舞夏, 後藤優樹, 玉井伸岳, 松木均 :
酸性リン脂質ジパルミトイルホスファチジン酸二重膜の熱的相転移,
第55回熱測定討論会, 2019年10月. 横矢祐香, 桶谷嘉一, 玉井伸岳, 後藤優樹, 松木均 :
対称型カチオン-アニオン界面活性剤塩の水中における二分子膜状会合体形成,
第55回熱測定討論会, 2019年10月. 本橋牧子, 青木雄椰, 田中杏奈, 玉井伸岳, 後藤優樹, 松木均 :
塩化ジアルキルジメチルアンモニウム二重膜の相転移熱力学量,
第55回熱測定討論会, 2019年10月. 玉井伸岳, 稲澤早苗, 後藤優樹, 松木均 :
リン脂質二分子膜の相挙動におよぼす種々のステロールの効果,
第55回熱測定討論会, 2019年10月. 玉井伸岳, 信岡健, 武知嶺, 後藤優樹, 松木均 :
動的および静的光散乱法によるリン脂質ベシクルの構造評価2,
第57回生物物理学会年会, 2019年9月. 玉井伸岳, 信岡健, 武知嶺, 後藤優樹, 松木均 :
動的・静的光散乱法によるリン脂質会合体の構造特性評価,
日本膜学会第41年会, 2019年5月. 松木均, 田中佐江子, 岡本裕嗣, 後藤優樹, 玉井伸岳 :
酸性リン脂質二分子膜の会合形態依存相転移,
日本膜学会第41年会, 2019年5月. 後藤優樹, 岡本裕嗣, 吉本早希, 吉田俊太郎, 玉井伸岳, 松木均 :
リン脂質二分子膜の圧力誘起相転移 -アシル鎖長の異なる飽和ジアシルホスファチジルグリセロール-,
第59回高圧討論会, 2018年11月. 吉田俊太郎, 後藤優樹, 玉井伸岳, 松木均 :
ホスファチジルコリン二重膜の圧力誘起指組み構造形成:高圧蛍光法による遷移状態の実証,
第59回高圧討論会, 2018年11月. 青木雄椰, 村上尚, 村上祐介, 田中杏奈, 後藤優樹, 玉井伸岳, 松木均 :
陽イオン性二本鎖界面活性剤二重膜の相転移に関する熱力学的研究,
第59回高圧討論会, 2018年11月. 平井悠貴, 後藤優樹, 玉井伸岳, 松木均 :
核磁気共鳴法によるホスファチジルコリン二分子膜中の脂質分子運動性評価,
2018年度日本化学会中国四国支部大会, 2018年11月. 清水建翔, 後藤優樹, 玉井伸岳, 松木均 :
カチオン-アニオン界面活性剤の水中における会合挙動,
2018年度日本化学会中国四国支部大会, 2018年11月. 田中杏奈, 青木雄椰, 後藤優樹, 玉井伸岳, 松木均 :
塩化テトラデシルジメチルアンモニウムの合成と二分子膜物性,
2018年度日本化学会中国四国支部大会, 2018年11月. 松木均, 風間暁, 後藤優樹, 玉井伸岳 :
脂質二分子膜の圧力誘起膜融合2:融合の分子メカニズム,
膜シンポジウム2018, 2018年11月. 松木均, 木口碧, 中尾俊樹, 本橋牧子, 後藤優樹, 玉井伸岳 :
リン脂質二分子膜のゲル-液晶中間相形成:充填状態依存相転移と形成機構,
第54回熱測定討論会, 2018年10月. 吉田俊太郎, 後藤優樹, 玉井伸岳, 松木均 :
ホスファチジルコリン二重膜の圧力誘起指組み構造形成:蛍光プローブ法による遷移状態の検出,
第35回九州コロイドコロキウム, 2018年9月. 木口碧, 後藤優樹, 玉井伸岳, 松木均 :
中鎖ホスファチジルコリン二分子膜の特異的な相転移:充填状態に依存したゲル-液晶転移,
第35回九州コロイドコロキウム, 2018年9月. 後藤優樹, 村上祐介, 村上尚, 後藤優樹, 松木均 :
塩化ジアルキルジメチルアンモニウム二重膜の高圧相挙動:アニーリング処理に依存した膜状態,
第69回コロイドおよび界面化学討論会, 2018年9月. 玉井伸岳, 森光達, 後藤優樹, 長宗秀明, 松木均 :
リン脂質-コレステロール混合二分子膜に対するコレステロール依存性細胞溶解毒素の結合活性,
第69回コロイドおよび界面化学討論会, 2018年9月. 玉井伸岳, 森光達, 後藤優樹, 長宗秀明, 松木均 :
モデル膜を用いたコレステロール依存性細胞溶解毒素の膜結合活性評価,
第56回生物物理学会年会, 2018年9月. 木口碧, 後藤優樹, 玉井伸岳, 松木均 :
中鎖ホスファチジルコリン二分子膜のゲル-液晶中間相形成,
第10回日本生物物理学会中国四国支部大会, 2018年5月. 吉田俊太郎, 後藤優樹, 玉井伸岳, 松木均 :
ホスファチジルコリン二分子膜における圧力誘起指組み構造形成の分子機構,
第10回日本生物物理学会中国四国支部大会, 2018年5月. 平井悠貴, 後藤優樹, 玉井伸岳, 松木均 :
エステルおよびエーテル結合型ホスファチジルコリン二分子膜中の脂質分子運動性評価,
第10回日本生物物理学会中国四国支部大会, 2018年5月. 松木均, 後藤優樹, 玉井伸岳 :
脂質二分子膜の圧力誘起膜融合,
日本膜学会第40年会, 2018年5月. 玉井伸岳, 森光達, 後藤優樹, 長宗秀明, 松木均 :
コレステロール依存性細胞溶解毒素の脂質膜に対する結合活性,
日本膜学会第40年会, 2018年5月. 松木均, 吉田俊太郎, 後藤優樹, 玉井伸岳 :
脂質二分子膜へのリガンド分配2:温度・圧力誘起相の安定性変化,
膜シンポジウム2017, 2017年11月. 竹下真広, 玉井伸岳, 後藤優樹, 松木均 :
リン脂質–コレステロール二成分混合二分子膜における各成分の部分モル体積,
膜シンポジウム2017, 2017年11月. 村上祐介, 村上尚, 後藤優樹, 玉井伸岳, 松木均 :
塩化ジアルキルジメチルアンモニウム二重膜の高圧相挙動,
第58回高圧討論会, 2017年11月. 後藤優樹, 吉田俊太郎, 玉井伸岳, 松木均 :
ジパルミトイルホスファチジルグリセロール二重膜の蛍光イメージング,
第58回高圧討論会, 2017年11月. 松木均, 岡本裕嗣, 吉田俊太郎, 後藤優樹, 玉井伸岳 :
リガンド存在下におけるリン脂質二分子膜の高圧相挙動:圧力誘起指組み構造ゲル相の安定性変化,
第58回高圧討論会, 2017年11月. 中尾俊樹, 木口碧, 後藤優樹, 玉井伸岳, 松木均 :
ジトリデカノイルホスファチジルコリン二分子膜における中間相の熱的相転移,
第53回熱測定討論会, 2017年11月. 後藤優樹, 岡本裕嗣, 中尾俊樹, 玉井伸岳, 松木均 :
リガンド添加による脂質二分子膜相転移温度変化の熱力学的解釈,
第55回生物物理学会年会, 2017年9月. 玉井伸岳, 泉川拓也, 植村麻衣子, 後藤優樹, 松木均 :
コレステロールによるジアルキルホスファチジルコリンの指組構造化の抑制,
第55回生物物理学会年会, 2017年9月. 村上尚, 村上祐介, 後藤優樹, 玉井伸岳, 松木均 :
二本鎖陽イオン性界面活性剤二重膜の高圧相挙動,
第68回コロイドおよび界面化学討論会, 2017年9月. 坪井俊祐, 後藤優樹, 玉井伸岳, 松木均 :
コレステロールを含む二成分リン脂質二分子膜の圧力摂動熱量測定を用いた体積挙動の定量的評価,
第68回コロイドおよび界面化学討論会, 2017年9月. 岡本裕嗣, 後藤優樹, 玉井伸岳, 松木均, 深田和宏 :
添加塩により誘起されるジステアロイルホスファチジルグリセロール二分子膜の特異的相転移,
第68回コロイドおよび界面化学討論会, 2017年9月. 後藤優樹, 村上尚, 村上祐介, 後藤優樹, 松木均 :
塩化ジアルキルジメチルアンモニウム二重膜の圧力誘起相転移,
第68回コロイドおよび界面化学討論会, 2017年9月. 玉井伸岳, 泉川拓也, 植村麻衣子, 後藤優樹, 松木均 :
ジアルキルリン脂質の指組構造形成におよぼすコレステロール効果,
第68回コロイドおよび界面化学討論会, 2017年9月. 松木均, 岡本裕嗣, 後藤優樹, 玉井伸岳 :
脂質二分子膜へのリガンド分配:相転移温度変化の熱力学的考察,
日本膜学会第39年会, 2017年5月. 玉井伸岳, 泉川拓也, 植村麻衣子, 後藤優樹, 松木均 :
エーテル結合型リン脂質-コレステロール二成分混合膜の相挙動,
日本膜学会第39年会, 2017年5月. 青木雄椰, 後藤優樹, 玉井伸岳, 松木均 :
N-メチル化ジヘキサデシルホスファチジルエタノールアミン二分子膜の高圧相挙動,
第9回日本生物物理学会中国四国支部大会, 2017年5月. 後藤優樹, 岡本裕嗣, 玉井伸岳, 松木均 :
ジパルミトイルホスファチジルグリセロール二重膜の圧力誘起相転移,
第9回日本生物物理学会中国四国支部大会, 2017年5月. 岡本裕嗣, 後藤優樹, 玉井伸岳, 松木均 :
ジアシルホスファチジルグリセロール二分子膜の添加塩誘起相転移,
第3回日本生物工学会西日本支部講演会, 2016年12月. 竹下真広, 玉井伸岳, 後藤優樹, 松木均 :
コレステロール含有二成分リン脂質二分子膜中における構成脂質の部分モル体積の組成依存的変化,
第3回日本生物工学会西日本支部講演会, 2016年12月. 後藤優樹, 遠藤茂, 玉井伸岳, 松木均 :
アシル鎖長の異なるホスファチジルエタノールアミン二重膜の熱および圧力相転移,
第54回生物物理学会年会, 2016年11月. 玉井伸岳, 竹下真広, 後藤優樹, 松木均 :
ジミリストイルホスファチジルコリン-コレステロール二成分二分子膜中における構成脂質の部分モル体積,
第54回生物物理学会年会, 2016年11月. 竹下真広, 玉井伸岳, 後藤優樹, 松木均 :
リン脂質-コレステロール混合二分子膜の体積挙動:部分モル体積の組成依存性,
2016年度日本化学会中国四国支部大会, 2016年11月. 坪井俊祐, 玉井伸岳, 後藤優樹, 松木均 :
リン脂質-コレステロール混合二分子膜の体積挙動:転移体積の組成依存性,
2016年度日本化学会中国四国支部大会, 2016年11月. 村上祐介, 後藤優樹, 玉井伸岳, 松木均 :
二本鎖陽イオン性界面活性剤二重膜の相挙動-アルキル鎖長の影響-,
2016年度日本化学会中国四国支部大会, 2016年11月. 村上尚, 後藤優樹, 玉井伸岳, 松木均 :
二本鎖陽イオン性界面活性剤二重膜の相挙動-対イオンの影響-,
2016年度日本化学会中国四国支部大会, 2016年11月. 後藤優樹, 玉井伸岳, Joachim Kohlbrecher, 松木均 :
陽イオン性二本鎖界面活性剤二重膜の構造解析,
2016年度日本化学会中国四国支部大会, 2016年11月. 後藤優樹, 岡本裕嗣, 玉井伸岳, 松木均 :
高圧力下におけるジパルミトイルホスファチジルグリセロール二重膜の相挙動,
第57回高圧討論会, 2016年10月. 松木均, 山下翔子, 後藤優樹, 玉井伸岳 :
飽和ジアシルホスファチジルエタノールアミン二重膜の相転移:疎水鎖長依存性と相転移熱力学量,
第57回高圧討論会, 2016年10月. 後藤優樹, 岡本裕嗣, 玉井伸岳, 松木均 :
ジパルミトイルホスファチジルグリセロール二重膜の相転移熱力学量,
第52回熱測定討論会, 2016年9月. 西本真琴, 高垣美由紀, 後藤優樹, 玉井伸岳, 松木均, 山中美智男 :
血清アルブミンと麻酔薬の本質的な結合様式:麻酔薬疎水性と特異的結合部位,
第52回熱測定討論会, 2016年9月. 村上祐介, 後藤優樹, 玉井伸岳, 松木均 :
二本鎖陽イオン性界面活性剤二重膜の相挙動におよぼすアルキル鎖長の影響,
第52回熱測定討論会, 2016年9月. 坪井俊祐, 玉井伸岳, 後藤優樹, 松木均 :
圧力摂動熱量測定を用いたジミリストイルホスファチジルコリン-コレステロール混合二分子膜の体積挙動の定量的評価,
第52回熱測定討論会, 2016年9月. 村上尚, 後藤優樹, 玉井伸岳, 松木均 :
二本鎖陽イオン性界面活性剤二重膜の相挙動におよぼす対イオンの影響,
第52回熱測定討論会, 2016年9月. 岡本裕嗣, 後藤優樹, 玉井伸岳, 松木均 :
ジアシルホスファチジルグリセロール二分子膜が形成する棒状会合体のゲル-液晶転移,
第52回熱測定討論会, 2016年9月. 玉井伸岳, 後藤優樹, 松木均 :
圧力摂動熱量法を利用したリン脂質二分子膜の体積特性の評価,
第52回熱測定討論会, 2016年9月. 玉井伸岳, 竹下真広, 坪井俊祐, 後藤優樹, 松木均 :
ジミリストイルホスファチジルコリン二分子膜の体積特性におよぼすコレステロール効果,
第67回コロイドおよび界面化学討論会, 2016年9月. 松木均, 山下翔子, 後藤優樹, 玉井伸岳 :
飽和ジアシルホスファチジルエタノールアミン二重膜の温度および圧力誘起相転移,
第67回コロイドおよび界面化学討論会, 2016年9月. 玉井伸岳, 坪井俊祐, 竹下真広, 後藤優樹, 松木均 :
ジミリストイルホスファチジルコリン-コレステロール混合二分子膜の体積挙動,
第8回日本生物物理学会中国四国支部大会, 2016年5月. 岡本裕嗣, 後藤優樹, 玉井伸岳, 松木均 :
ジステアロイルホスファチジルグリセロール二分子膜の添加塩濃度に依存した特異的な相転移,
第8回日本生物物理学会中国四国支部大会, 2016年5月. 松木均, 加藤謙太郎, 後藤優樹, 玉井伸岳 :
高圧力下におけるリン脂質二重膜のリガンド分配様式,
第56回高圧討論会, 2015年11月. 後藤優樹, 玉井伸岳, 松木均 :
リン脂質二重膜の圧力誘起相転移–酸性リン脂質ジパルミトイルホスファチジルグリセロール–,
第56回高圧討論会, 2015年11月. 後藤優樹 :
高圧蛍光法による脂質二重膜内充填構造の評価,
第38回溶液化学シンポジウムプレシンポジウム, 2015年10月. 井上嶺之, 後藤優樹, 玉井伸岳, 松木均 :
酸性リン脂質二重膜の温度・圧力誘起相転移:ジミリストイルホスファチジルグリセロール,
第51回熱測定討論会, 2015年10月. 黒葛和信, 後藤優樹, 玉井伸岳, 松木均 :
血清アルブミン中における麻酔薬の結合部位:熱的および構造データの相関性,
第51回熱測定討論会, 2015年10月. 後藤優樹, 玉井伸岳, 松木均 :
非対称飽和アシル鎖を有するホスファチジルコリンの温度および圧力誘起二重膜相転移,
第53回日本生物物理学会年会, 2015年9月. 後藤優樹, 玉井伸岳, Joachim Kohlbrecher, 松木均 :
高圧小角中性子散乱法による二本鎖界面活性剤会合体の構造解析,
第66回コロイドおよび界面化学討論会, 2015年9月. 松木均, 後藤優樹, 玉井伸岳 :
生体膜脂質の非対称性:非対称飽和ホスファチジルコリン位置異性体,
特殊環境微生物セミナー2015, 2015年9月. 後藤優樹 :
支部発海外だより∼スイス・ポールシェラー研究所滞在記∼,
日本生物物理学会第7回中国四国支部大会, 2015年5月. 黒葛和信, 後藤優樹, 玉井伸岳, 西本真琴, 松木均 :
血清アルブミン中における麻酔薬の結合部位:脂肪酸の結合部位との同一性,
第7回日本生物物理学会中国四国支部大会, 2015年5月. 井上嶺之, 後藤優樹, 玉井伸岳, 松木均 :
酸性リン脂質二重幕の熱的相転移:ジミリストイルホスファチジルグリセロール,
第7回日本生物物理学会中国四国支部大会, 2015年5月. 玉井伸岳, 後藤優樹, 松木均 :
圧力摂動熱量法を用いたリン脂質二分子膜の体積挙動の定量的評価,
日本膜学会第37年会, 2015年5月. 松木均, 矢野貴大, 後藤優樹, 玉井伸岳 :
脂質膜の圧力誘起指組み構造形成(その2):アシル鎖非対称性,
日本膜学会第37年会, 2015年5月. 八木信久, 後藤優樹, 玉井伸岳, 松木均 :
高圧下におけるリン脂質二分子膜の体積挙動 ―高圧精密密度測定システムの構築―,
日本膜学会第37年会, 2015年5月. 井上嶺之, 後藤優樹, 玉井伸岳, 松木均 :
ホスファチジルグリセロール二分子膜の熱的相挙動:相転移におよぼす電荷の影響,
日本膜学会第37年会, 2015年5月. 茨木孝司, 後藤優樹, 玉井伸岳, 松木均 :
リン脂質二重膜の圧力誘起膜融合 -融合率と封入率の相関性-,
第55回高圧討論会, 2014年11月. 玉井伸岳, 後藤優樹, 松木均 :
リン脂質二分子膜の体積挙動,
第55回高圧討論会, 2014年11月. 矢野貴大, 後藤優樹, 玉井伸岳, 松木均 :
リン脂質二分子膜の圧力誘起相転移 -非対称飽和アシル鎖をもつホスファチジルコリン-,
第55回高圧討論会, 2014年11月. 松木均, 後藤優樹, 玉井伸岳 :
リン脂質二重膜の圧力および化学誘起指組み構造化:形成機構の相違,
第52回生物物理学会年会, 2014年9月. 玉井伸岳, 信岡健, 後藤優樹, 松木均 :
動的および静的光散乱法によるリン脂質ベシクルの構造評価,
第52回生物物理学会年会, 2014年9月. 玉井伸岳, 森絵里香, 後藤優樹, 松木均 :
リン脂質ベシクルの体積緩和挙動におよぼすコレステロール効果,
第65回コロイドおよび界面化学討論会, 2014年9月. 松木均, 金澤雅俊, 後藤優樹, 玉井伸岳 :
高圧力が誘起するリン脂質二重膜の膜融合:膜曲率の効果と融合機構,
第65回コロイドおよび界面化学討論会, 2014年9月. 矢野貴大, 後藤優樹, 玉井伸岳, 松木均 :
脂質二分子膜相安定性におよぼすアシル鎖非対称性の影響,
第6回日本生物物理学会中国四国支部大会, 2014年5月. 松木均, 後藤優樹, 玉井伸岳 :
脂質膜の圧力誘起指組み構造形成:短鎖限界と長鎖限界,
日本膜学会第36年会, 2014年5月. 玉井伸岳, 後藤優樹, 松木均 :
コレステロールが誘起するジパルミトイルホスファチジルコリン二重膜の側方相分離,
日本膜学会第36年会, 2014年5月. 松木均, 多田佳織, 後藤優樹, 玉井伸岳 :
リン脂質二分子膜の圧力誘起相転移 -ジラウロイル基を有するリン脂質-,
第54回高圧討論会, 2013年11月. 後藤優樹, 佐藤明弘, 玉井伸岳, 松木均 :
リン脂質二重膜のサブゲル相形成 -エーテル結合およびエステル結合型リン脂質の相違-,
第54回高圧討論会, 2013年11月. 西本真琴, 矢野華奈子, 後藤優樹, 玉井伸岳, 松木均 :
ホタル発光酵素ルシフェラーゼへの麻酔薬効果:DSC測定による構造安定性の評価,
第49回熱測定討論会, 2013年11月. 松木均, 黒葛和信, 後藤優樹, 玉井伸岳, 西本真琴 :
牛血清アルブミンへの麻酔薬効果:DSC，ITCおよびPPC測定による比較研究,
第49回熱測定討論会, 2013年11月. 後藤優樹, 玉井伸岳, 松木均 :
高圧蛍光法により明らかにされるサブゲル相中のホスファチジルコリン分子のスタッガード構造,
第51回生物物理学会年会, 2013年10月. 玉井伸岳, 稲澤早苗, 藤原大樹, 後藤優樹, 松木均 :
ジパルミトイルホスファチジルコリン二分子膜の熱的相挙動に及ぼすステロール効果,
第51回生物物理学会年会, 2013年10月. 松木均, 後藤優樹, 玉井伸岳 :
疎水性相互作用に支配される脂質膜の圧力誘起指組み構造形成,
特殊環境微生物セミナー2013, 2013年10月. 茨木孝司, 後藤優樹, 玉井伸岳, 松木均 :
圧力誘起膜融合リポソームの安定性評価,
第64回コロイドおよび界面化学討論会, 2013年9月. 矢野貴大, 森神佳彦, 後藤優樹, 玉井伸岳, 松木均 :
非対称型ホスファチジルコリン二分子膜の高圧誘起相転移,
第64回コロイドおよび界面化学討論会, 2013年9月. 大坪洋平, 岩佐悠太, 後藤優樹, 玉井伸岳, 松木均 :
ハロゲン化ジオクタデシルジメチルアンモニウム二重膜の高圧相挙動,
第64回コロイドおよび界面化学討論会, 2013年9月. 後藤優樹, Agnieszka Wilk, 玉井伸岳, Joachim Kohlbrecher, 松木均 :
ホスファチジルコリン二分子膜における圧力誘起指組み構造の分子メカニズム,
第64回コロイドおよび界面化学討論会, 2013年9月. 玉井伸岳, 柿部小百合, 田中佐江子, 後藤優樹, 松木均 :
圧力摂動熱量法によるリン脂質二分子膜の緩和挙動の観測,
第64回コロイドおよび界面化学討論会, 2013年9月. 松木均, 後藤優樹, 玉井伸岳 :
高圧力が誘起するリン脂質二重膜の膜融合,
第64回コロイドおよび界面化学討論会, 2013年9月. 松木均, 後藤優樹, 玉井伸岳 :
高圧力により誘起されるリン脂質二重膜の膜融合(第2報),
第18回生物関連高圧研究会シンポジウム, 2013年9月. 松木均, 西本真琴, 後藤優樹, 玉井伸岳 :
麻酔作用のタンパク質受容体仮説の源泉:ホタル発光酵素ルシフェラーゼ,
第5回日本生物物理学会中国四国支部大会, 2013年5月. 玉井伸岳, 後藤優樹, 松木均 :
脂質ラフト形成は熱力学的に説明できるのか? -側方相分離とクラスター形成-,
第5回日本生物物理学会中国四国支部大会, 2013年5月. 後藤優樹, 玉井伸岳, 松木均 :
リン脂質二重膜のイメージング:蛍光分子の膜内配向で見る膜状態,
第5回日本生物物理学会中国四国支部大会, 2013年5月. 後藤優樹, Agnieszka Wilk, 玉井伸岳, Joachim Kohlbrecher, 松木均 :
リン脂質二重膜のサブゲル相形成 -非対称型リン脂質膜への高圧蛍光法の適用-,
第53回高圧討論会, 2012年11月. 松木均, 金澤雅俊, 後藤優樹, 玉井伸岳 :
リン脂質二重膜の圧力誘起膜融合 -巨大単層ベシクルの不可逆的球形成長-,
第53回高圧討論会, 2012年11月. 松木均, 後藤優樹, 玉井伸岳 :
リン脂質二重膜の圧力誘起指組み構造形成:疎水鎖長依存性と形成限界,
第50回生物物理学会年会, 2012年9月. 玉井伸岳, 柿部小百合, 田中佐江子, 後藤優樹, 松木均 :
圧力摂動熱量法によるジミリストイルホスファチジルコリン二分子膜の緩和挙動の解明への試み,
第50回生物物理学会年会, 2012年9月. 玉井伸岳, 稲澤早苗, 藤原大樹, 後藤優樹, 松木均 :
エルゴステロール–ジパルミトイルホスファチジルコリン二成分混合二分子膜の相挙動,
第4回日本生物物理学会中国四国支部大会, 2012年6月. 後藤優樹, Agnieszka Wilk, 玉井伸岳, Joachim Kohlbrecher, 松木均 :
ジアシルホスファチジルコリン二重膜の圧力誘起指組み構造化の形成限界,
第4回日本生物物理学会中国四国支部大会, 2012年6月. 福井誠, SA Mulyatno, 後藤優樹, 三木かなめ, RC Orihuela-Campos, 片岡宏介, 林田秀明, 北村雅保, 川崎浩二, 関田孝晴, 中里未央, 前田隆浩, 齋藤俊行, 伊藤博夫 :
ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性-第二報-,
第61回日本口腔衛生学会・総会, 2012年5月. 松木均, 後藤優樹, 玉井伸岳 :
脂質膜の圧力誘起指組み構造形成:疎水鎖長依存性と形成限界,
材料学会第61期通常総会講演会極限環境フォーラム, 2012年5月. 森神佳彦, 田中佐江子, 澤口裕史, 後藤優樹, 玉井伸岳, 松木均 :
ジアシルホスファチジルコリン二分子膜のラメラ結晶相形成におよぼすアシル鎖長の効果,
2011年日本化学会西日本大会, 2011年11月. 田岡昌朗, 後藤優樹, 玉井伸岳, 松木均 :
ジアシルホスファチジルコリン二分子膜の指組み構造化のメカニズム,
2011年日本化学会西日本大会, 2011年11月. 田中佐江子, 後藤優樹, 玉井伸岳, 松木均 :
ジパルミトイルホスファチジルグリセロール二分子膜の相挙動に及ぼすNaClの効果,
2011年日本化学会西日本大会, 2011年11月. 金澤雅俊, 後藤優樹, 玉井伸岳, 松木均 :
非対称型リン脂質二分子膜相挙動におよぼすベシクルサイズの効果,
2011年日本化学会西日本大会, 2011年11月. 稲澤早苗, 後藤優樹, 玉井伸岳, 松木均 :
ジパルミトイルホスファチジルコリン二分子膜相挙動に及ぼすスティグマステロールの効果,
2011年日本化学会西日本大会, 2011年11月. 遠藤卓也, 田中佐江子, 後藤優樹, 玉井伸岳, 松木均 :
長鎖飽和アシル鎖を有するホスファチジルコリン二分子膜の高圧相挙動,
2011年日本化学会西日本大会, 2011年11月. 岩佐悠太, 後藤優樹, 玉井伸岳, 松木均 :
臭化および塩化ジオクタデシルジメチルアンモニウム二分子膜相挙動におよぼす1-ブタノールの影響,
2011年日本化学会西日本大会, 2011年11月. 玉井伸岳, 南部優子, 後藤優樹, 松木均 :
圧力摂動法による脂質二分子膜体積挙動の定量的解析,
第52回高圧討論会, 2011年11月. 後藤優樹, Agnieszka Wilk, 遠藤卓也, 田中佐江子, 玉井伸岳, Joachim Kohlbrecher, 松木均 :
長鎖アシル鎖を有するホスファチジルコリン二分子膜の高圧相挙動,
第52回高圧討論会, 2011年11月. 福井誠, ムルヤトノサプタ, バータルジャフツェルメグ, 後藤優樹, 片岡宏介, 林田秀明, 川崎浩二, 前田隆浩, 中里未央, 齋藤俊行, 伊藤博夫 :
ヒト唾液中および血中の抗ホスホリルコリン抗体と動脈硬化リスクとの関連性:五島研究,
2011年10月. ムルヤトノサプタ, 片岡宏介, 関根伸一, バータルジャブツェルメグ, 福井誠, 金川裕子, 後藤優樹, 伊藤博夫 :
経鼻粘膜アジュバントCpG ODNはマクロファージのTLR9発現と歯周病細菌抗原特異的Th1免疫応答を誘導する,
2011年10月. 松木均, 後藤優樹, 玉井伸岳 :
高圧力により誘起されるリン脂質二重膜の膜融合,
第17回生物関連高圧研究会シンポジウム, 2011年9月. 玉井伸岳, 南部優子, 後藤優樹, 松木均 :
圧力摂動法によるエステルおよびエーテル結合型リン脂質膜の体積挙動の解明,
第3回日本生物物理学会中国四国支部大会, 2011年5月. 松木均, 後藤優樹, 玉井伸岳 :
脂質二重膜内パッキング状態のイメージング解析,
第3回日本生物物理学会中国四国支部大会, 2011年5月. 梨野翔, 後藤優樹, 玉井伸岳, 松木均 :
リン脂質-短鎖脂肪酸混合二分子膜の相挙動,
2010年日本化学会西日本大会, 2010年11月. 衣川進, 多田佳織, 後藤優樹, 玉井伸岳, 松木均, 金品昌志 :
カチオン-アニオン界面活性剤混合系の会合体転移に関する熱的研究,
2010年日本化学会西日本大会, 2010年11月. 後藤優樹, 玉井伸岳, 松木均, 金品昌志 :
ジアシルホスファチジルコリン二分子膜相挙動の蛍光イメージング解析,
第51回高圧討論会, 2010年10月. 風間暁, Agnieszka Wilk, 後藤優樹, 玉井伸岳, 金品昌志, Joachim Kohlbrecher, 松木均 :
ホスファチジルコリン二分子膜の圧力誘起指組み構造化のメカニズム,
第51回高圧討論会, 2010年10月. 玉井伸岳, 泉川拓也, 後藤優樹, 松木均 :
ジヘキサデシルホスファチジルコリン二分子膜の相挙動に及ぼすコレステロールの影響,
第2回日本生物物理学会中国四国支部大会, 2010年5月. 筒井舞子, 後藤優樹, 玉井伸岳, 松木均 :
飽和・不飽和リン脂質二分子膜の体積挙動観測,
第2回日本生物物理学会中国四国支部大会, 2010年5月. 田中佐江子, 後藤優樹, 玉井伸岳, 松木均 :
ジパルミトイルホファチジルグリセロール二分子膜の高圧相挙動,
第2回日本生物物理学会中国四国支部大会, 2010年5月.

研究会・報告書

日下一也, 山田洋平, 大西舞, 塚越雅幸, 安澤幹人, 後藤優樹, 森本恵美, 芥川正武, 寺田賢治, 藤澤正一郎 :
導入科目「プロジェクトマネジメント基礎」の実施と評価,
電気学会研究会資料制御研究会, Vol.CT-14, No.15, 11-16, 2014年4月.

(要約): We report about the introductory subject "Project Management Fundamentals". The student will get a basic ability to work with diverse peoples in the community or workplace, through the planning and practice of a project to accomplish the task. In addition, the student will obtain project management skills required to design and implement the project. This year's theme was "the development of new products to eliminate the inconvenience for you". The students worked on the development of new products in a team as a project. We evaluated the individual and team by using the project notes.
(キーワード): 導入科目 / 創成科目 / プロジェクト / プロジェクト管理 (project management) / グループ活動 / 評価 (evaluation)

特許: 後藤優樹, 玉井伸岳, 松木均 : 高圧力を利用したリポソームの粒子径制御方法, 特願2011-021310 (2011年2月), .
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: リピッドミューテーションによる極性基転置型非天然リン脂質の創製と会合体挙動 (研究課題/領域番号: 21K05006 )
モジュール構造変更アナログ脂質の二重膜キャラクタリゼーション (研究課題/領域番号: 19K05404 )
脂質膜のメカノアシンメトリー：圧力研究から見えてくる脂質分子非対称性 (研究課題/領域番号: 26410016 )
吸入麻酔薬は本当に受容体結合薬物であるのか：ホタル発光酵素のカロリメトリー (研究課題/領域番号: 24659698 )
ステロール－脂質二重膜の体積緩和ダイナミクスとミクロドメインの動的形成機構 (研究課題/領域番号: 24550157 )
ホスホリルコリンに対する粘膜免疫応答と動脈硬化との関連性 (研究課題/領域番号: 23792515 )
研究者番号（30507455）による検索

その他: 研究者総覧に該当データはありませんでした。

研究者総覧で最新データを確認する

2024年11月22日更新

専門分野・研究分野: 生物物理化学 (Biophysical Chemistry)
所属学会・所属協会: 社団法人日本化学会
日本高圧力学会
日本口腔衛生学会
日本麻酔メカニズム研究会
日本生物物理学会
委員歴・役員歴: 研究者総覧に該当データはありませんでした。
受賞: 2003年9月, 第56回コロイドおよび界面化学討論会ポスター賞 (社団法人日本化学会)
2011年7月, Best Poster Award (The 6th International Conference on Advanced Materials Development and Performance)
2011年12月, Selected Paper of the Bulletin Chemical Society of Japan (社団法人日本化学会)
2012年11月, 奨励賞 (日本高圧力学会)
2014年11月, 第55回高圧討論会ポスター賞 (日本高圧力学会)
2016年9月, 第52回熱測定討論会ポスター賞(渦潮賞) (日本熱測定学会)
2017年9月, 第68回コロイドおよび界面化学討論会ポスター賞 (社団法人日本化学会)
2019年11月, 2019年度日本化学会中国四国支部大会ポスター賞 (日本化学会中国四国支部)
2021年6月, 日本膜学会第43年会学生賞 (日本膜学会)
2021年9月, 第35回九州コロイドコロキウム優秀ポスター賞 (日本化学会コロイドおよび界面化学部会九州支部)
2022年8月, 第36回九州コロイドコロキウム優秀ポスター賞 (日本化学会コロイドおよび界面化学部会九州支部)
2022年12月, 第63回高圧討論会ポスター賞 (日本高圧力学会)
2023年9月, 第74回コロイドおよび界面化学討論会ポスター賞 (社団法人日本化学会)
2023年12月, 2023年度麻酔メカニズム研究会優秀ポスター賞 (日本麻酔メカニズム研究会)
2024年5月, 第15回日本生物物理学会中国四国支部大会若手発表優秀賞 (日本生物物理学会中国四国支部)
2024年9月, 第60回熱測定討論会ポスター賞(京都北山賞) (日本熱測定学会)
活動: 研究者総覧に該当データはありませんでした。

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2024年11月17日更新

2024年11月16日更新

Ｊグローバル

Jグローバル最終確認日: 2024/11/16 01:20
氏名（漢字）: 後藤優樹
氏名（フリガナ）: ゴトウマサキ
氏名（英字）: GOTO Masaki
所属機関: 徳島大学准教授
徳島大学准教授

リサーチマップ

researchmap最終確認日

2024/11/17 01:32

氏名（漢字）

後藤優樹

氏名（フリガナ）

ゴトウマサキ

氏名（英字）

GOTO Masaki

プロフィール

京都は伏見出身、ＵＳクリーブランド＆博多育ち。発話障害に苦しむも、生物工学を志し阿波徳島へ。三度の無職を経て徳島大ライフシステム部門に着任(松木研)。自身の研究テーマは一貫して「高圧力と脂質ナノ粒子(LNP)」。高圧力技術を武器にLNPの物性解明と産業的応用を模索している。リン脂質二重膜の高圧相挙動から研究を始め、その蛍光イメージングと放射光による構造解析、二本鎖界面活性剤二重膜の高圧物性、圧力誘起膜融合現象、新規非天然型リン脂質の有機合成とその二重膜物性、麻酔薬のLNPに対する作用メカニズムなどの研究を遂行中である。また予防歯科学教室・医局在籍時に学んだ歯学・免疫学を生かし異分野との融合も試みている。

研究室ホームページ

https://biomembeng-tokushima-u.com/

京都市美術館

https://kyotocity-kyocera.museum/

登録日時

2013/7/3 08:35

更新日時

2024/10/4 19:32

アバター画像URI

https://researchmap.jp/masaki_goto/avatar.jpg

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徳島大学

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大学院社会産業理工学研究部生物資源産業学域応用生命系応用生物資源学分野

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准教授

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博士（工学）

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徳島大学

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ＫＡＫＥＮで最新データを確認する

2024年11月16日更新

研究者番号: 30507455

所属（現在）: 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授

所属（過去の研究課題 情報に基づく）*注記: 2021/4/1 – 2022/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授
2019/4/1 – 2020/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 助教
2016/4/1 : 徳島大学, 大学院生物資源産業学研究部, 助教
2011/4/1 – 2015/4/1 : 徳島大学, ソシオテクノサイエンス研究部, 助教
2014/4/1 : 徳島大学, 大学院ソシオテクノサイエンス研究部, 助教
2013/4/1 : 徳島大学, 大学院ソシオテクノサイエンス研究部, 助教
2012/4/1 : 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教
2011/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教

審査区分/研究分野

研究代表者

生物系 / 医歯薬学 / 歯学 / 社会系歯学

研究代表者以外

生物系 / 医歯薬学 / 歯学 / 社会系歯学
理工系 / 化学 / 複合化学 / 機能物質化学
生物系 / 医歯薬学 / 外科系臨床医学 / 麻酔・蘇生学
理工系 / 化学 / 基礎化学 / 物理化学
小区分32020:機能物性化学関連

キーワード

研究代表者

粘膜免疫 / ホスホリルコリン / 動脈硬化 / 口腔常在菌

研究代表者以外

歯周病 / 予防ワクチン / 唾液タンパク質 / 粘膜免疫 / 分泌型IgA抗体 / 定着阻害 / 唾液 / リン脂質二分子膜 / コレステロール / 圧力摂動熱量法（PPC） / 高圧密度測定 / 等温圧縮率 / 緩和挙動 / ルシフェラーゼ / 吸入麻酔薬 / 脂肪酸 / アデノシン３リン酸 / 示差走査熱量測定 / 等温滴定熱量測定 / 特異的および非特異的相互作用 / 誘導適合 / 長鎖脂肪酸 / 圧力摂動熱量測定 / 脂質膜 / 高圧力 / 相転移 / 指組み構造 / 非対称脂質 / ホスファチジルコリン / メカノアシンメトリー / 膜内イメージング / モジュール変更アナログ脂質 / グリセロリン脂質 / スフィンゴリン脂質 / 脂質二重膜 / 熱測定 / モジュール構造変更アナログ脂質 / 非天然脂質 / 非天然リン脂質 / リン脂質二重膜 / 高圧相挙動 / 示差走査熱量法 / 二重膜相転移

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後藤 優樹

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研究代表者

研究代表者以外

研究代表者

研究代表者以外

後藤優樹