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川上竜巳

徳島大学

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リサーチマップ・
Jグローバル
KAKEN
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2024年4月26日更新

職名: 准教授
電話: 088-656-7247
電子メール: kawakami@tokushima-u.ac.jp
学歴: 2004/3: 徳島大学大学院工学研究科機能システム工学専攻修了
学位: 博士(工学) (徳島大学) (2004年3月)
職歴・経歴: 2004/4: 徳島大学工学部教務補佐員
2006/4: 九州大学大学院農学研究院学術研究員
2006/10: 佐賀大学総合分析実験センター助手
2007/4: 佐賀大学総合分析実験センター助教

専門分野・研究分野: 酵素化学 (Enzyme Chemistry)

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2024年4月26日更新

専門分野・研究分野: 酵素化学 (Enzyme Chemistry)
担当経験のある授業科目: インターンシップ (学部)
フードサイエンス (学部)
卒業研究 (学部)
基礎食品化学 (学部)
生物資源学研究 (大学院)
生物資源産業学B (学部)
生物資源産業学実習 (学部)
生物資源産業学専門英語 (学部)
産業体験実習 (学部)
英語論文講読 (学部)
英語論文講読Ⅰ (学部)
英語論文講読Ⅱ (学部)
遺伝子工学 (学部)
遺伝子資源利用学 (学部)
酵素化学 (学部)
酵素化学特論 (大学院)
食と健康概論 (学部)
食料生物科学特別実習 (大学院)
食料生物科学特別演習 (大学院)
食料生物科学特別研究 (大学院)
食料生物科学特別講義 (大学院)
食料科学基礎実習 (学部)
食料科学実習A (学部)
食料科学実習B (学部)
食料科学実習C (学部)
食料科学実習Ⅰ (学部)
食料科学実習Ⅱ (学部)
食料科学概論 (学部)
指導経験: 9人 (学士), 5人 (修士)

研究者総覧で最新データを確認する

2024年4月26日更新

専門分野・研究分野: 酵素化学 (Enzyme Chemistry)

研究テーマ: 極限環境微生物酵素の機能・構造・応用 (超好熱アーキア, アミノ酸脱水素酵素, アミノ酸ラセマーゼ, タンパク質結晶化, X線結晶構造解析)

著書

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論文

Hanif ALi, Mone Yamanishi, Keigo Sunagawa, Mizuki Kumon, Rumana Yesmin Hasi, Mutsumi Aihara, Ryushi Kawakami and Tamotsu Tanaka :
Protective effect of oleic acid against very long-chain fatty acid-induced apoptosis in peroxisome-deficient CHO cells,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1869, No.3, 159452, 2024.

(要約): Very long-chain fatty acids (VLCFAs) are degraded exclusively in peroxisomes, as evidenced by the accumulation of VLCFAs in patients with certain peroxisomal disorders. Although accumulation of VLCFAs is considered to be associated with health issues, including neuronal degeneration, the mechanisms underlying VLCFAs-induced tissue degeneration remain unclear. Here, we report the toxic effect of VLCFA and protective effect of C18: 1 FA in peroxisome-deficient CHO cells. We examined the cytotoxicity of saturated and monounsaturated VLCFAs with chain-length at C20-C26, and found that longer and saturated VLCFA showed potent cytotoxicity at lower accumulation levels. Furthermore, the extent of VLCFA-induced toxicity was found to be associated with a decrease in cellular C18:1 FA levels. Notably, supplementation with C18:1 FA effectively rescued the cells from VLCFA-induced apoptosis without reducing the cellular VLCFAs levels, implying that peroxisome-deficient cells can survive in the presence of accumulated VLCFA, as long as the cells keep sufficient levels of cellular C18:1 FA. These results suggest a therapeutic potential of C18:1 FA in peroxisome disease and may provide new insights into the pharmacological effect of Lorenzo's oil, a 4:1 mixture of C18:1 and C22:1 FA.
(徳島大学機関リポジトリ): ● Metadata: 118916
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2024.159452
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 38244676; ● Search Scopus @ Elsevier (PMID): 38244676; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2024.159452

(徳島大学機関リポジトリ: 118916, DOI: 10.1016/j.bbalip.2024.159452, PubMed: 38244676) Yoshimichi Takai, Rumana Yesmin Hasi, Naoko Matsumoto, Chiho Fujita, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Mutsumi Aihara, Toshiki Ishikawa, Hiroyuki Imai, Mayuko Wakida, Kazuya Ando and Tamotsu Tanaka :
Degradation of glycosylinositol phosphoceramide during plant tissue homogenization,
The Journal of Biochemistry, Vol.175, No.1, 115-124, 2024.

(徳島大学機関リポジトリ): ● Metadata: 118914
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvad080
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1093/jb/mvad080

(徳島大学機関リポジトリ: 118914, DOI: 10.1093/jb/mvad080) Ryushi Kawakami, Takami Naoki, Junji Hayashi, Kazunari Yoneda, Ohmori Taketo, Toshihisa Ohshima and Haruhiko Sakuraba :
First crystal structure of an NADP+-dependent L-arginine dehydrogenase belonging to the μ-crystallin family,
International Journal of Biological Macromolecules, Vol.249, 2023.

(キーワード): L-Arginine dehydrogenase / μ-Crystallin/ornithine cyclodeaminase family / Crystal structure / Amino acid dehydrogenase / Site-directed mutagenesis
(徳島大学機関リポジトリ): ● Metadata: 119010
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijbiomac.2023.126070
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.ijbiomac.2023.126070

(徳島大学機関リポジトリ: 119010, DOI: 10.1016/j.ijbiomac.2023.126070) Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Peroxisomes attenuate cytotoxicity of very long-chain fatty acids,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1868, No.2, 159259, 2023.

(要約): One of the major functions of peroxisomes in mammals is oxidation of very long-chain fatty acids (VLCFAs). Genetic defects in peroxisomal β-oxidation result in the accumulation of VLCFAs and lead to a variety of health problems, such as demyelination of nervous tissues. However, the mechanisms by which VLCFAs cause tissue degeneration have not been fully elucidated. Recently, we found that the addition of small amounts of isopropanol can enhance the solubility of saturated VLCFAs in an aqueous medium. In this study, we characterized the biological effect of extracellular VLCFAs in peroxisome-deficient Chinese hamster ovary (CHO) cells, neural crest-derived pheochromocytoma cells (PC12), and immortalized adult Fischer rat Schwann cells (IFRS1) using this solubilizing technique. C20:0 FA was the most toxic of the C16-C26 FAs tested in all cells. The basis of the toxicity of C20:0 FA was apoptosis and was observed at 5 μM and 30 μM in peroxisome-deficient and wild-type CHO cells, respectively. The sensitivity of wild-type CHO cells to cytotoxic C20:0 FA was enhanced in the presence of a peroxisomal β-oxidation inhibitor. Further, a positive correlation was evident between cell toxicity and the extent of intracellular accumulation of toxic FA. These results suggest that peroxisomes are pivotal in the detoxification of apoptotic VLCFAs by preventing their accumulation.
(キーワード): Cricetinae / Animals / Peroxisomes / Fatty Acids / CHO Cells / Cricetulus / Oxidation-Reduction
(徳島大学機関リポジトリ): ● Metadata: 117825
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2022.159259
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36460260; ● Search Scopus @ Elsevier (PMID): 36460260; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2022.159259

(徳島大学機関リポジトリ: 117825, DOI: 10.1016/j.bbalip.2022.159259, PubMed: 36460260) Rumana Yesmin Hasi, Toshiki Ishikawa, Keigo Sunagawa, Yoshimichi Takai, Hanif Ali, Junji Hayashi, Ryushi Kawakami, Keizo Yuasa, Mutsumi Aihara, Kaori Kanemaru, Hiroyuki Imai and Tamotsu Tanaka :
Nonspecific phospholipase C3 of radish has phospholipase D activity toward glycosylinositol phosphoceramide,
FEBS Letters, Vol.596, No.23, 3024-3036, 2022.

(要約): Glycosylinositol phosphoceramide (GIPC) is a major sphingolipid in the plasma membranes of plants. Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of the D position of GIPC in cabbage and named this activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as a PLD. The recombinant NPC3 protein obtained by heterologous expression system in Escherichia coli produced PC1P from GIPC and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish and Arabidopsis thaliana. From these results, we conclude that NPC3 is one of the enzymes that degrade GIPC.
(キーワード): Phospholipase D / Raphanus / Phospholipases / Sphingolipids / Brassica / Arabidopsis
(徳島大学機関リポジトリ): ● Metadata: 117824
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/1873-3468.14520
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36266963; ● Search Scopus @ Elsevier (PMID): 36266963; ● Search Scopus @ Elsevier (DOI): 10.1002/1873-3468.14520

(徳島大学機関リポジトリ: 117824, DOI: 10.1002/1873-3468.14520, PubMed: 36266963) Ryushi Kawakami, Tatsuya Ohshida, Junji Hayashi, Kazunari Yoneda, Toshio Furumoto, Toshihisa Ohshima and Haruhiko Sakuraba :
Crystal structure of a novel type of ornithine δ-aminotransferase from the hyperthermophilic archaeon Pyrococcus horikoshii,
International Journal of Biological Macromolecules, Vol.208, 731-740, 2022.

(要約): Ornithine δ-aminotransferase (Orn-AT) activity was detected for the enzyme annotated as a γ-aminobutyrate aminotransferase encoded by PH1423 gene from Pyrococcus horikoshii OT-3. Crystal structures of this novel archaeal ω-aminotransferase were determined for the enzyme in complex with pyridoxal 5'-phosphate (PLP), in complex with PLP and l-ornithine (l-Orn), and in complex with N-(5'-phosphopyridoxyl)-l-glutamate (PLP-l-Glu). Although the sequence identity was relatively low (28%), the main-chain coordinates of P. horikoshii Orn-AT monomer showed notable similarity to those of human Orn-AT. However, the residues recognizing the α-amino group of l-Orn differ between the two enzymes. In human Orn-AT, Tyr55 and Tyr85 recognize the α-amino group, whereas the side chains of Thr92* and Asp93*, which arise from a loop in the neighboring subunit, form hydrogen bonds with the α-amino group of the substrate in P. horikoshii enzyme. Site-directed mutagenesis suggested that Asp93* plays critical roles in maintaining high affinity for the substrate. This study provides new insight into the substrate binding of a novel type of Orn-AT. Moreover, the structure of the enzyme with the reaction-intermediate analogue PLP-l-Glu bound provides the first structural evidence for the "Glu switch" mechanism in the dual substrate specificity of Orn-AT.
(キーワード): Archaea / Crystallography, X-Ray / Humans / Models, Molecular / Ornithine / Pyridoxal Phosphate / Pyrococcus horikoshii / Substrate Specificity / Transaminases
(徳島大学機関リポジトリ): ● Metadata: 119006
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ijbiomac.2022.03.114
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 35337912; ● Summary page in Scopus @ Elsevier: 2-s2.0-85127367273

(徳島大学機関リポジトリ: 119006, DOI: 10.1016/j.ijbiomac.2022.03.114, PubMed: 35337912, Elsevier: Scopus) Ali Hanif, Morito Katsuya, Rumana Hasi Yesmin, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Sango Kazunori and Tamotsu Tanaka :
Characterization of uptake and metabolism of very long-chain fatty acids in peroxisome-deficient CHO cells,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol.1867, No.2, 159088, 2022.

(要約): Fatty acids (FAs) longer than C20 are classified as very long-chain fatty acids (VLCFAs). Although biosynthesis and degradation of VLCFAs are important for the development and integrity of the myelin sheath, knowledge on the incorporation of extracellular VLCFAs into the cells is limited due to the experimental difficulty of solubilizing them. In this study, we found that a small amount of isopropanol solubilized VLCFAs in aqueous medium by facilitating the formation of the VLCFA/albumin complex. Using this solubilizing technique, we examined the role of the peroxisome in the uptake and metabolism of VLCFAs in Chinese hamster ovary (CHO) cells. When wild-type CHO cells were incubated with saturated VLCFAs (S-VLCFAs), such as C23:0 FA, C24:0 FA, and C26:0 FA, extensive uptake was observed. Most of the incorporated S-VLCFAs were oxidatively degraded without acylation into cellular lipids. In contrast, in peroxisome-deficient CHO cells uptake of S-VLCFAs was marginal and oxidative metabolism was not observed. Extensive uptake and acylation of monounsaturated (MU)-VLCFAs, such as C24:1 FA and C22:1 FA, were observed in both types of CHO cells. However, oxidative metabolism was evident only in wild-type cells. Similar manners of uptake and metabolism of S-VLCFAs and MU-VLCFAs were observed in IFRS1, a Schwan cell-derived cell line. These results indicate that peroxisome-deficient cells limit intracellular S-VLCFAs at a low level by halting uptake, and as a result, peroxisome-deficient cells almost completely lose the clearance ability of S-VLCFAs accumulated outside of the cells.
(徳島大学機関リポジトリ): ● Metadata: 116579
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbalip.2021.159088
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34848380; ● Search Scopus @ Elsevier (PMID): 34848380; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbalip.2021.159088

(徳島大学機関リポジトリ: 116579, DOI: 10.1016/j.bbalip.2021.159088, PubMed: 34848380) Hanif Ali, Ryouhei Yamashita, Jun-ichi Morishige, Katsuya Morito, Naoya Kakiuchi, Junji Hayashi, Mutsumi Aihara, Ryushi Kawakami, Koichiro Tsuchiya and Tamotsu Tanaka :
Massspectrometric analysis of sphingomyelin with N-alfa-hydroxy fatty acyl residue in mouse tissues,
Lipids, Vol.56, No.2, 181-188, 2021.

(徳島大学機関リポジトリ): ● Metadata: 117826
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/lipd.12285
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1002/lipd.12285

(徳島大学機関リポジトリ: 117826, DOI: 10.1002/lipd.12285) Ryushi Kawakami, Chinatsu Kinoshita, Tomoki Kawase, Mikio Sato, Junji Hayashi, Haruhiko Sakuraba and Toshihisa Ohshima :
Characterization of novel moderate-substrate specificity amino acid racemase from the hyperthermophilic archaeon Thermococcus litoralis.,
Bioscience, Biotechnology, and Biochemistry, Vol.85, No.7, 1650-1657, 2021.

(要約): The amino acid sequence of the OCC_10945 gene product from the hyperthermophilic archaeon Thermococcus litoralis DSM5473, originally annotated as γ-aminobutyrate aminotransferase, is highly similar to that of the uncharacterized pyridoxal 5'-phosphate (PLP)-dependent amino acid racemase from Pyrococcus horikoshii. The OCC_10945 enzyme was successfully overexpressed in Escherichia coli by coexpression with a chaperone protein. The purified enzyme demonstrated PLP-dependent amino acid racemase activity primarily toward Met and Leu. Although PLP contributed to enzyme stability, it only loosely bound to this enzyme. Enzyme activity was strongly inhibited by several metal ions, including Co2+ and Zn2+, and nonsubstrate amino acids such as l-Arg and l-Lys. These results suggest that the underlying PLP-binding and substrate recognition mechanisms in this enzyme are significantly different from those of the other archaeal and bacterial amino acid racemases. This is the first description of a novel PLP-dependent amino acid racemase with moderate substrate specificity in hyperthermophilic archaea.
(キーワード): PLP / amino acid racemase / substrate specificity / Thermococcus litoralis
(徳島大学機関リポジトリ): ● Metadata: 116135
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/bbb/zbab078
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33942867; ● Search Scopus @ Elsevier (PMID): 33942867; ● Search Scopus @ Elsevier (DOI): 10.1093/bbb/zbab078

(徳島大学機関リポジトリ: 116135, DOI: 10.1093/bbb/zbab078, PubMed: 33942867) Rumana Yesmin Hasi, Dai Majima, Katsuya Morito, Hanif Ali, Kentaro Kogure, Meera Nanjundan, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities.,
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, Vol.1152, 122213, 2020.

(要約): Glycosylinositol phosphoceramide (GIPC) is a sphingophospholipid in plants. Recently, we identified that GIPC is hydrolyzed to phytoceramide 1-phosphate (PC1P) by an uncharacterized phospholipase D activity following homogenization of certain plant tissues. We now developed methods for isolation of GIPC and PC1P from plant tissues and characterized their chemical stabilities. Hydrophilic solvents, namely a lower layer of a mixed solvent system consisting of isopropanol/hexane/water (55:20:25, v/v/v) was efficient solvent for extraction and eluent in column chromatography. GIPC was isolated by Sephadex column chromatography followed by TLC. A conventional method, such as the Bligh and Dyer method, was applicable for PC1P extraction. Specifically, PC1P was isolated by TLC following mild alkali treatment of lipid extracts of plants. The yields of GIPC and PC1P in our methods were both around 50-70%. We found that PC1P is tolerant against heat (up to 125 °C), strong acid (up to 10 M HCl), and mild alkali (0.1 M KOH). In contrast, significant degradation of GIPC occurred at 100 °C and 1.0 M HCl treatment, suggesting the instability of the inositol glycan moiety in these conditions. These data will be useful for further biochemical and nutritional studies on these sphingolipids.
(徳島大学機関リポジトリ): ● Metadata: 115195
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jchromb.2020.122213
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32615533; ● Search Scopus @ Elsevier (PMID): 32615533; ● Search Scopus @ Elsevier (DOI): 10.1016/j.jchromb.2020.122213

(徳島大学機関リポジトリ: 115195, DOI: 10.1016/j.jchromb.2020.122213, PubMed: 32615533) 川上竜巳 :
超好熱アーキアのFAD依存性脱水素酵素複合体及びPLP依存性アミノ酸ラセマーゼの研究,
ビタミン, Vol.93, No.12, 531-541, 2019年.

(要約): 著者らはこれまで，アミノ酸代謝経路の分析やバイオセンサー素子としての酵素の応用利用のために，超好熱菌由来酵素のスクリーニングや機能解析を進めてきた．この総合論文では，これまでに見出した2種類の酵素，FAD依存性L-プロリン脱水素酵素複合体とPLP依存性アミノ酸ラセマーゼについて紹介する．まず，電気化学的バイオセンサー用の機能性素子として利用可能な安定な色素依存性脱水素酵素をスクリーニングする過程で2種類の異なるL-プロリン脱水素酵素活性を見出した．最初に見出したThermococcus profundus由来の酵素はαβγδ構造の複合体酵素であり，L-プロリン脱水素酵素活性だけでなく，NADH脱水素酵素活性も示した．この酵素を用いたDNAセンシングシステムの構築にも成功している．Pyrococcus horikoshiiから見出した第2の酵素もα4β4構造の複合体酵素であり，補因子としてFAD，FMN，ATP，鉄を有していた．一方で，低基質特異性を示すPLP依存性アミノ酸ラセマーゼはP. horikoshiiの増殖におけるD-アミノ酸資化性を調査する過程で見つかった．さらに著者らは，ホモログ酵素の機能解析によってAlaとSerに特異的なアミノ酸ラセマーゼも見出した．これらの脱水素酵素やラセマーゼは両方とも，Thermococcales目超好熱アーキアに広く保存されており，アミノ酸代謝において重要な役割を担っていると考えられる．
(キーワード): Hyperthermophilic archaea / L-proline dehydrogenase complex / amino acid racemase / flavin cofactor / Pyridoxal-5'-phosphate
(徳島大学機関リポジトリ): ● Metadata: 115046
(出版サイトへのリンク): ● Publication site (DOI): 10.20632/vso.93.12_531
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390286981364692224; ● Search Scopus @ Elsevier (DOI): 10.20632/vso.93.12_531

(徳島大学機関リポジトリ: 115046, DOI: 10.20632/vso.93.12_531, CiNii: 1390286981364692224) Rumana Yesmin Hasi, Makoto Miyagi, Takashi Kida, Tatsuya Fukuta, Kentaro Kogure, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Quantitative Analysis of Glycosylinositol Phosphoceramide and Phytoceramide 1-Phosphate in Vegetables,
Journal of Nutritional Science and Vitaminology, Vol.65, No.Supplement, S175-S179, 2019.

(要約): Previously, we found an unidentified sphingolipid in cabbage, and determined it as phytoceramide 1-phosphate (PC1P). PC1P is found to be produced from glycosylinositol phosphoceramide (GIPC) by the action of phospholipase D (PLD) activity. Although GIPC is abundant sphingolipid, especially in cruciferous vegetables, amount of daily intake, digestibility and nutritional activity of GIPC are not well understood. Here, we investigated amounts of GIPC and PC1P in vegetables. GIPC was found in all vegetables examined (13 kinds) at levels 3-20 mg/100 g (wet weight). On the other hand, PC1P was present in limited vegetables which show higher GIPC-PLD activity, such as inner cabbage leaves (5.2 mg/100 g). Because PC1P is formed during homogenization by activated GIPC-PLD, level of PC1P in boiled cabbage leaves was very low. Although digestibility of GIPC is unknown at present, a portion of dietary GIPC is considered to be converted to PC1P during mastication by plant-derived GIPC-PLD activity in some vegetables.
(キーワード): Brassica / Ceramides / Glycosphingolipids / Inositol / Phosphates / Phospholipase D / Plant Leaves / Sphingolipids / Vegetables
(徳島大学機関リポジトリ): ● Metadata: 115197
(出版サイトへのリンク): ● Publication site (DOI): 10.3177/jnsv.65.S175
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31619623; ● Summary page in Scopus @ Elsevier: 2-s2.0-85073476996

(徳島大学機関リポジトリ: 115197, DOI: 10.3177/jnsv.65.S175, PubMed: 31619623, Elsevier: Scopus) Hasi Yesmin Rumana, Makoto Miyagi, Katsuya Morito, Toshiki Ishikawa, Maki Kawai-Yamada, Hiroyuki Imai, Tatsuya Fukuta, Kentaro Kogure, Kaori Kanemaru, Junji Hayashi, Ryushi Kawakami and Tamotsu Tanaka :
Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation,
The Journal of Biochemistry, Vol.166, No.5, 441-448, 2019.

(要約): Glycosylinositol phosphoceramide (GIPC) is the most abundant sphingolipid in plants and fungi. Recently, we detected GIPC-specific phospholipase D (GIPC-PLD) activity in plants. Here, we found that GIPC-PLD activity in young cabbage leaves catalyzes transphosphatidylation. The available alcohol for this reaction is a primary alcohol with a chain length below C4. Neither secondary alcohol, tertiary alcohol, choline, serine nor glycerol serves as an acceptor for transphosphatidylation of GIPC-PLD. We also found that cabbage GIPC-PLD prefers GIPC containing two sugars. Neither inositol phosphoceramide, mannosylinositol phosphoceramide nor GIPC with three sugar chains served as substrate. GIPC-PLD will become a useful catalyst for modification of polar head group of sphingophospholipid.
(キーワード): Biocatalysis / Brassica / Ceramides / Inositol / Molecular Structure / Phosphatidylcholines / Phospholipase D / Plant Leaves
(徳島大学機関リポジトリ): ● Metadata: 113685
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvz056
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 31504617; ● Summary page in Scopus @ Elsevier: 2-s2.0-85073764089

(徳島大学機関リポジトリ: 113685, DOI: 10.1093/jb/mvz056, PubMed: 31504617, Elsevier: Scopus) Ryushi Kawakami, Tatsuya Ohshida, Haruhiko Sakuraba and Toshihisa Ohshima :
A novel PLP-dependent Alanine/Serine racemase from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3,
Frontiers in Microbiology, 2018.

(要約): We recently identified and characterized a novel broad substrate specificity amino acidracemase (BAR) from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3.Three genes, PH0782, PH1423, and PH1501, encoding homologs exhibiting about45% sequence identity with BAR were present in the P. horikoshii genome. In this study,we detected pyridoxal 50-phosphate (PLP)-dependent amino acid racemase activity inthe protein encoded by PH0782. The enzyme showed activity toward Ala, Ser, Thr,and Val, but the catalytic efficiency with Thr or Val was much lower than with Ala orSer. The enzyme was therefore designated Ala/Ser racemase (ASR). Like BAR, ASRwas highly stable at high temperatures and over a wide range of pHs, though itshexameric structure differed from the dimeric structure of BAR. No activity was detectedin K291A or D234A ASR mutants. This suggests that, as in Ile 2-epimerase (ILEP) fromLactobacillus buchneri JCM1115, these residues are involved in Schiff base formationand substrate interaction, respectively. Unlike BAR, enhanced ASR activity was notdetected in P. horikoshii cells cultivated in the presence of D-Ala or D-Ser. This is thefirst description of a PLP-dependent fold type I ASR in archaea.
(キーワード): Ala racemase / Ser racemase / PLP-dependent enzyme / Pyrococcus horikoshii / hyperthermophilic archaea / D-amino acid
(徳島大学機関リポジトリ): ● Metadata: 114287
(出版サイトへのリンク): ● Publication site (DOI): 10.3389/fmicb.2018.01481
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85049861690

(徳島大学機関リポジトリ: 114287, DOI: 10.3389/fmicb.2018.01481, Elsevier: Scopus) Ryushi Kawakami, Haruhiko Sakuraba, Ohmori Taketo and Toshihisa Ohshima :
First characterization of an archaeal amino acid racemase with broad substrate specificity from the hyperthermophile Pyrococcus horikoshii OT-3 induced by D-amino acids,
Journal of Bioscience and Bioengineering, Vol.124, No.1, 23-27, 2017.

(要約): A novel amino acid racemase with broad substrate specificity (BAR) was recently isolated from the hyperthermophilic archaeon Pyrococcus horikoshii OT-3. Characterization of this enzyme has been difficult, however, because the recombinant enzyme is produced mainly as an inclusion body in Escherichia coli. In this study, expression of the recombinant protein into the soluble fraction was markedly improved by co-expression with chaperone molecules. The purified enzyme retained its full activity after incubation at 80°C for at least 2h in buffer (pH 710), making this enzyme the most thermostable amino acid racemase so far known. Besides the nine amino acids containing hydrophobic and aromatic amino acids previously reported (Kawakami etal., Amino Acids, 47, 15791587, 2015), the enzyme exhibited substantial activity toward Thr (about 42% of relative activity toward Phe) and showed no activity toward Arg, His, Gln, and Asn. The substrate specificity of this enzyme thus differs markedly from those of other known amino acid racemases. In particular, the high reaction rate with Trp and Tyr, in addition to Leu, Met and Phe as substrates is a noteworthy feature of this enzyme. The high reactivity toward Trp and Tyr, as well as extremely high thermostability, is likely a major advantage of using BAR for biochemical conversion of these aromatic amino acids.
(キーワード): Amino Acid Isomerases / Amino Acid Sequence / Hydrophobic and Hydrophilic Interactions / Pyrococcus horikoshii / Substrate Specificity / Temperature
(徳島大学機関リポジトリ): ● Metadata: 111977
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.jbiosc.2017.02.004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 28343923; ● Summary page in Scopus @ Elsevier: 2-s2.0-85016976917

(徳島大学機関リポジトリ: 111977, DOI: 10.1016/j.jbiosc.2017.02.004, PubMed: 28343923, Elsevier: Scopus) Badr Ali Hoida, Takahashi Keiko, Ryushi Kawakami, Yasuo Oyama, Kumio Yokoigawa and Kaori Kanemaru :
Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin.,
European Food Research and Technology, Vol.243, No.12, 2147-2153, 2017.

(キーワード): Shiga toxin / Seaweed / Ulva linza Linnaeus / Rhamnan sulfate
(徳島大学機関リポジトリ): ● Metadata: 111019
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00217-017-2915-1
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1007/s00217-017-2915-1

(徳島大学機関リポジトリ: 111019, DOI: 10.1007/s00217-017-2915-1) Tatsuya Ohshida, Junji Hayashi, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
First characterization of extremely halophilic 2-deoxy-D-ribose-5-phosphate aldolase,
Protein Expression and Purification, Vol.126, 62-68, 2016.

(要約): 2-Deoxy-d-ribose-5-phosphate aldolase (DERA) catalyzes the aldol reaction between two aldehydes and is thought to be a potential biocatalyst for the production of a variety of stereo-specific materials. A gene encoding DERA from the extreme halophilic archaeon, Haloarcula japonica, was overexpressed in Escherichia coli. The gene product was successfully purified, using procedures based on the protein's halophilicity, and characterized. The expressed enzyme was stable in a buffer containing 2 M NaCl and exhibited high thermostability, retaining more than 90% of its activity after heating at 70 °C for 10 min. The enzyme was also tolerant to high concentrations of organic solvents, such as acetonitrile and dimethylsulfoxide. Moreover, H. japonica DERA was highly resistant to a high concentration of acetaldehyde and retained about 35% of its initial activity after 5-h' exposure to 300 mM acetaldehyde at 25 °C, the conditions under which E. coli DERA is completely inactivated. The enzyme exhibited much higher activity at 25 °C than the previously characterized hyperthermophilic DERAs (Sakuraba et al., 2007). Our results suggest that the extremely halophilic DERA has high potential to serve as a biocatalyst in organic syntheses. This is the first description of the biochemical characterization of a halophilic DERA.
(徳島大学機関リポジトリ): ● Metadata: 111972
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.pep.2016.05.009
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27215670; ● Summary page in Scopus @ Elsevier: 2-s2.0-84973496063

(徳島大学機関リポジトリ: 111972, DOI: 10.1016/j.pep.2016.05.009, PubMed: 27215670, Elsevier: Scopus) Ryushi Kawakami, Taketo Ohmori, Haruhiko Sakuraba and Toshihisa Ohshima :
Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids,
Amino Acids, Vol.47, No.8, 1579-1587, 2015.

(要約): To date, there have been few reports analyzing the amino acid requirement for growth of hyperthermophilic archaea. We here found that the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 requires Thr, Leu, Val, Phe, Tyr, Trp, His and Arg in the medium for growth, and shows slow growth in medium lacking Met or Ile. This largely corresponds to the presence, or absence, of genes related to amino acid biosynthesis in its genome, though there are exceptions. The amino acid requirements were dramatically lost by addition of D-isomers of Met, Leu, Val, allo-Ile, Phe, Tyr, Trp and Arg. Tracer analysis using (14)C-labeled D-Trp showed that D-Trp in the medium was used as a protein component in the cells, suggesting the presence of D-amino acid metabolic enzymes. Pyridoxal 5'-phosphate (PLP)-dependent racemase activity toward Met, Leu and Phe was detected in crude extract of P. horikoshii and was enhanced in cells grown in the medium supplemented with D-amino acids, especially D-allo-Ile. The gene encoding the racemase was narrowed down to one open reading frame on the basis of enzyme purification from P. horikoshii cells, and the recombinant enzyme exhibited PLP-dependent racemase activity toward several amino acids, including Met, Leu and Phe, but not Pro, Asp or Glu. This is the first report showing the presence in a hyperthermophilic archaeon of a PLP-dependent amino acid racemase with broad substrate specificity that is likely responsible for utilization of D-amino acids for growth.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00726-015-2001-6
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25963389; ● Summary page in Scopus @ Elsevier: 2-s2.0-84937518494

(DOI: 10.1007/s00726-015-2001-6, PubMed: 25963389, Elsevier: Scopus) Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Identification of catalytic residues of a very large NAD-glutamate dehydrogenase from Janthinobacterium lividum by site-directed mutagenesis,
Bioscience, Biotechnology, and Biochemistry, Vol.78, No.12, 2045-2050, 2014.

(要約): We previously found a very large NAD-dependent glutamate dehydrogenase with approximately 170 kDa subunit from Janthinobacterium lividum (Jl-GDH) and predicted that GDH reaction occurred in the central domain of the subunit. To gain further insights into the role of the central domain, several single point mutations were introduced. The enzyme activity was completely lost in all single mutants of R784A, K810A, K820A, D885A, and S1142A. Because, in sequence alignment analysis, these residues corresponded to the residues responsible for glutamate binding in well-known small GDH with approximately 50 kDa subunit, very large GDH and well-known small GDH may share the same catalytic mechanism. In addition, we demonstrated that C1141, one of the three cysteine residues in the central domain, was responsible for the inhibition of enzyme activity by HgCl2, and HgCl2 functioned as an activating compound for a C1141T mutant. At low concentrations, moreover, HgCl2 was found to function as an activating compound for a wild-type Jl-GDH. This suggests that the mechanism for the activation is entirely different from that for the inhibition.
(キーワード): Amino Acid Sequence / Bacterial Proteins / Binding Sites / Biocatalysis / Burkholderiaceae / Cysteine / Enzyme Assays / Gene Expression / Glutamate Dehydrogenase / Glutamic Acid / Kinetics / Mercuric Chloride / Molecular Sequence Data / Mutagenesis, Site-Directed / Mutation / Protein Binding / Protein Structure, Tertiary / Protein Subunits / Sequence Homology, Amino Acid / Structure-Activity Relationship
(出版サイトへのリンク): ● Publication site (DOI): 10.1080/09168451.2014.946394
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 25126984; ● Summary page in Scopus @ Elsevier: 2-s2.0-84924529811

(DOI: 10.1080/09168451.2014.946394, PubMed: 25126984, Elsevier: Scopus) Ryushi Kawakami, Chiaki Noguchi, Marie Higashi, Haruhiko Sakuraba and Toshihisa Ohshima :
Comparative analysis of the catalytic components in the archaeal dye-linked L-proline dehydrogenase complexes.,
Applied Microbiology and Biotechnology, Vol.97, No.8, 3419-3427, 2013.

(要約): Two types of hetero-oligomeric dye-linked L-proline dehydrogenases (44 and types) are expressed in the hyperthermophilic archaea belonging to Thermococcales. In both enzymes, the subunit (PDH) is responsible for catalyzing L-proline dehydrogenation. The genes encoding the two enzyme types form respective clusters that are completely conserved among Pyrococcus and Thermococcus strains. To compare the enzymatic properties of PDHs from 44- and -type enzyme complexes, eight PDHs (four of each type) from Pyrococcus furiosus DSM3638, Pyrococcus horikoshii OT-3, Thermococcus kodakaraensis KOD1 JCM12380 and Thermococcus profundus DSM9503 were expressed in Escherichia coli cells and purified to homogeneity using one-step Ni-chelating chromatography. The 44-type PDHs showed greater thermostability than most of the -type PDHs: the former retained more than 80 % of their activity after heating at 70 °C for 20 min, while the latter showed different thermostabilities under the same conditions. In addition, the 44-type PDHs utilized ferricyanide as the most preferable electron acceptor, whereas -type PDHs preferred 2, 6-dichloroindophenol, with one exception. These results indicate that the differences in the enzymatic properties of the PDHs likely reflect whether they were from an - or 44-type complex, though the wider divergence observed within -type PDHs based on the phylogenetic analysis may also be responsible for their inconsistent enzymatic properties. By contrast, differences in the kinetic parameters among the PDHs did not reflect the complex type. Interestingly, the k cat value for free 44-type PDH from P. horikoshii was much larger than the value for the same subunit within the 44-complex. This indicates that the isolated PDH could be a useful element for an electrochemical system for detection of L-proline.
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00253-012-4201-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22752365; ● Search Scopus @ Elsevier (PMID): 22752365; ● Search Scopus @ Elsevier (DOI): 10.1007/s00253-012-4201-2

(DOI: 10.1007/s00253-012-4201-2, PubMed: 22752365) Haruhiko Sakuraba, Takenori Satomura, Ryushi Kawakami, Kwang Kim, Yusuke Hara, Kazunari Yoneda and Toshihisa Ohshima :
Crystal structure of novel dye-linked L-proline dehydrogenase from hyperthermophilic archaeon Aeropyrum pernix.,
The Journal of Biological Chemistry, Vol.287, No.24, 20070-20080, 2012.

(要約): Two types of dye-linked L-proline dehydrogenase (PDH1, α4β4-type hetero-octamer, and PDH2, αβγδ-type heterotetramer) have been identified so far in hyperthermophilic archaea. Here, we report the crystal structure of a third type of L-proline dehydrogenase, found in the aerobic hyperthermophilic archaeon Aeropyrum pernix, whose structure (homodimer) is much simpler than those of previously studied L-proline dehydrogenases. The structure was determined at a resolution of 1.92 Å. The asymmetric unit contained one subunit, and a crystallographic 2-fold axis generated the functional dimer. The overall fold of the subunit showed similarity to that of the PDH1 β-subunit, which is responsible for catalyzing L-proline dehydrogenation. However, the situation at the subunit-subunit interface of the A. pernix enzyme was totally different from that in PDH1. The presence of additional surface elements in the A. pernix enzyme contributes to a unique dimer association. Moreover, the C-terminal Leu(428), which is provided by a tail extending from the FAD-binding domain, shielded the active site, and an L-proline molecule was entrapped within the active site cavity. The K(m) value of a Leu(428) deletion mutant for L-proline was about 800 times larger than the K(m) value of the wild-type enzyme, although the k(cat) values did not differ much between the two enzymes. This suggests the C-terminal Leu(428) is not directly involved in catalysis, but it is essential for maintaining a high affinity for the substrate. This is the first description of an LPDH structure with L-proline bound, and it provides new insight into the substrate binding of LPDH.
(キーワード): Aeropyrum / Archaeal Proteins / Crystallography, X-Ray / Proline Oxidase / Protein Multimerization / Protein Structure, Quaternary / Protein Structure, Tertiary
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M111.319038
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22511758; ● Search Scopus @ Elsevier (PMID): 22511758; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M111.319038

(DOI: 10.1074/jbc.M111.319038, PubMed: 22511758) Takenori Satomura, Akihiro Hiraki, Tomoyuki Kawai, Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
Expression, purification, crystallization and preliminary X-ray diffraction analysis of a galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum.,
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, Vol.68, No.Pt 3, 330-332, 2012.

(要約): A galactose 1-phosphate uridylyltransferase from the hyperthermophilic archaeon Pyrobaculum aerophilum was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the tetragonal space group P4(1), with unit-cell parameters a = b = 73.3, c = 126.1 Å, and diffracted to 2.73 Å resolution on beamline BL5A at the Photon Factory. The overall R(merge) was 7.3% and the data completeness was 99.8%.
(キーワード): Crystallization / Crystallography, X-Ray / Gene Expression / Pyrobaculum / UTP-Hexose-1-Phosphate Uridylyltransferase
(出版サイトへのリンク): ● Publication site (DOI): 10.1107/S1744309112003880
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22442236; ● Search Scopus @ Elsevier (PMID): 22442236; ● Search Scopus @ Elsevier (DOI): 10.1107/S1744309112003880

(DOI: 10.1107/S1744309112003880, PubMed: 22442236) Ryushi Kawakami, Takenori Satomura, Haruhiko Sakuraba and Toshihisa Ohshima :
L-proline dehydrogenases in hyperthermophilic archaea: distribution, function, structure, and application.,
Applied Microbiology and Biotechnology, Vol.93, No.1, 83-93, 2011.

(要約): Dye-linked L-proline dehydrogenase (ProDH) catalyzes the oxidation of L-proline to ∆(1)-pyrroline-5-carboxylate (P5C) in the presence of artificial electron acceptors. The enzyme is known to be widely distributed in bacteria and eukarya, together with nicotinamide adenine dinucleotide (phosphate)-dependent P5C dehydrogenase, and to function in the metabolism of L-proline to L-glutamate. In addition, over the course of the last decade, three other types of ProDH with molecular compositions completely different from previously known ones have been identified in hyperthermophilic archaea. The first is a heterotetrameric αβγδ-type ProDH, which exhibits both ProDH and reduced nicotinamide adenine dinucleotide dehydrogenase activity and includes two electron transfer proteins. The second is a heterooctameric α(4)β(4)-type ProDH, which uses flavin adenine dinucleotide, flavin mononucleotide, adenosine triphosphate, and Fe as cofactors and creates a new electron transfer pathway. The third is a recently identified homodimeric ProDH, which exhibits the greatest thermostability among these archaeal ProDHs. This minireview focuses on the functional and structural properties of these three types of archaeal ProDH and their distribution in archaea. In addition, we will describe the specific application of hyperthermostable ProDH for use in a biosensor and for DNA sensing.
(キーワード): Amino Acid Sequence / Archaea / Coenzymes / Models, Molecular / Molecular Sequence Data / Proline / Proline Oxidase / Protein Multimerization / Protein Subunits / Pyrroles
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00253-011-3682-8
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22089387; ● Search Scopus @ Elsevier (PMID): 22089387; ● Search Scopus @ Elsevier (DOI): 10.1007/s00253-011-3682-8

(DOI: 10.1007/s00253-011-3682-8, PubMed: 22089387) Takenori Shibahara, Takenori Satomura, Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix.,
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, Vol.67, No.Pt 11, 1425-1427, 2011.

(要約): A dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was crystallized using the hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the monoclinic space group P2(1), with unit-cell parameters a = 63.4, b = 119.4, c = 70.2 Å, β = 112.0°, and diffracted to 2.0 Å resolution on the BL26B1 beamline at SPring-8. The overall R(merge) was 4.5% and the completeness was 99.8%.
(キーワード): Aeropyrum / Crystallization / Crystallography, X-Ray / Lactate Dehydrogenases
(出版サイトへのリンク): ● Publication site (DOI): 10.1107/S1744309111036098
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 22102248; ● Search Scopus @ Elsevier (PMID): 22102248; ● Search Scopus @ Elsevier (DOI): 10.1107/S1744309111036098

(DOI: 10.1107/S1744309111036098, PubMed: 22102248) Haitao Zheng, Leyi Lin, Yosuke Okezaki, Ryushi Kawakami, Haruhiko Sakuraba, Toshihisa Ohshima, Keiichi Takagi and Shin-Ichiro Suye :
Electrochemical behavior of dye-linked L-proline dehydrogenase on glassy carbon electrodes modified by multi-walled carbon nanotubes.,
Beilstein Journal of Nanotechnology, Vol.1, 135-141, 2010.

(要約): A glassy carbon electrode (GC) was modified by multi-walled carbon nanotubes (MWCNTs). The modified electrode showed a pair of redox peaks that resulted from the oxygen-containing functional groups on the nanotube surface. A recombinant thermostable dye-linked L-proline dehydrogenase (L-proDH) from hyperthermophilic archaeon (Thermococcus profundus) was further immobilized by physical adsorption. The modified electrode (GC/MWCNTs/L-proDH) exhibited an electrocatalytic signal for L-proline compared to bare GC, GC/L-proDH and GC/MWCNTs electrodes, which suggested that the presence of MWCNTs efficiently enhances electron transfer between the active site of enzyme and electrode surface. The immobilized L-proDH showed a typical Michaelis-Menten catalytic response with lower apparent constant.
(出版サイトへのリンク): ● Publication site (DOI): 10.3762/bjnano.1.16
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21977403; ● Search Scopus @ Elsevier (PMID): 21977403; ● Search Scopus @ Elsevier (DOI): 10.3762/bjnano.1.16

(DOI: 10.3762/bjnano.1.16, PubMed: 21977403) Ryushi Kawakami, Masaki Oyama, Haruhiko Sakuraba and Toshihisa Ohshima :
The unique kinetic behavior of the very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum.,
Bioscience, Biotechnology, and Biochemistry, Vol.74, No.4, 884-887, 2010.

(要約): The kinetics of a very large NAD-dependent glutamate dehydrogenase from Janthinobacterium lividum showed positive cooperativity toward alpha-ketoglutarate and NADH, and the Michaelis-Menten type toward ammonium chloride in the absence of the catalytic activator, L-aspartate. An increase in the maximum activity accompanied the decrease in the S(0.5) values for alpha-ketoglutarate and NADH with the addition of L-aspartate, and the kinetic response for alpha-ketoglutarate changed completely to a typical Michaelis-Menten type in the presence of 10 mM L-aspartate.
(キーワード): Aspartic Acid / Catalysis / Chromobacterium / Glutamate Dehydrogenase / Glutamic Acid / Ketoglutaric Acids / Kinetics / NAD / Oxalobacteraceae
(出版サイトへのリンク): ● Publication site (DOI): 10.1271/bbb.90925
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20378971; ● CiNii @ 国立情報学研究所 (CRID): 1390001206478731904; ● Search Scopus @ Elsevier (PMID): 20378971; ● Search Scopus @ Elsevier (DOI): 10.1271/bbb.90925

(DOI: 10.1271/bbb.90925, PubMed: 20378971, CiNii: 1390001206478731904) Ryushi Kawakami, Haruhiko Sakuraba, Shuichiro Goda, Hideaki Tsuge and Toshihisa Ohshima :
Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix.,
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol.1794, No.10, 1496-1504, 2009.

(要約): Tartrate oxidation activity was found in the crude extract of an aerobic hyperthermophilic archaeon Aeropyrum pernix, and the enzyme was identified as (S)-malate dehydrogenase (MDH), which, when produced in Escherichia coli, was mainly obtained as an inactive inclusion body. The inclusion body was dissolved in 6 M guanidine-HCl and gradually refolded to the active enzyme through dilution of the denaturant. The purified recombinant enzyme consisted of four identical subunits with a molecular mass of about 110 kDa. NADP was preferred as a coenzyme over NAD for (S)-malate oxidation and, unlike MDHs from other sources, this enzyme readily catalyzed the oxidation of (2S,3S)-tartrate and (2S,3R)-tartrate. The tartrate oxidation activity was also observed in MDHs from the hyperthermophilic archaea Methanocaldococcus jannaschii and Archaeoglobus fulgidus, suggesting these hyperthermophilic MDHs loosely bind their substrates. The refolded A. pernix MDH was also crystallized, and the structure was determined at a resolution of 2.9 A. Its overall structure was similar to those of the M. jannaschii, Chloroflexus aurantiacus, Chlorobium vibrioforme and Cryptosporidium parvum [lactate dehydrogenase-like] MDHs with root-mean-square-deviation values between 1.4 and 2.1 A. Consistent with earlier reports, Ala at position 53 was responsible for coenzyme specificity, and the next residue, Arg, was important for NADP binding. Structural comparison revealed that the hyperthermostability of the A. pernix MDH is likely attributable to its smaller cavity volume and larger numbers of ion pairs and ion-pair networks, but the molecular strategy for thermostability may be specific for each enzyme.
(キーワード): Aeropyrum / Amino Acid Sequence / Base Sequence / Catalytic Domain / Crystallography, X-Ray / DNA Primers / DNA, Archaeal / Enzyme Stability / Genes, Archaeal / Malate Dehydrogenase / Models, Molecular / Molecular Sequence Data / Molecular Weight / Protein Folding / Protein Subunits / Recombinant Proteins / Sequence Homology, Amino Acid / Substrate Specificity / Thermodynamics
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbapap.2009.06.014
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19555779; ● Search Scopus @ Elsevier (PMID): 19555779; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbapap.2009.06.014

(DOI: 10.1016/j.bbapap.2009.06.014, PubMed: 19555779) Haruhiko Sakuraba, Kazunari Yoneda, Takenori Satomura, Ryushi Kawakami and Toshihisa Ohshima :
Structure of a D-tagatose 3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima.,
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, Vol.65, No.Pt 3, 199-203, 2009.

(要約): The crystal structure of a D-tagatose 3-epimerase-related protein (TM0416p) encoded by the hypothetical open reading frame TM0416 in the genome of the hyperthermophilic bacterium Thermotoga maritima was determined at a resolution of 2.2 A. The asymmetric unit contained two homologous subunits and a dimer was generated by twofold symmetry. The main-chain coordinates of the enzyme monomer proved to be similar to those of D-tagatose 3-epimerase from Pseudomonas cichorii and D-psicose 3-epimerase from Agrobacterium tumefaciens; however, TM0416p exhibited a unique solvent-accessible substrate-binding pocket that reflected the absence of an alpha-helix that covers the active-site cleft in the two aforementioned ketohexose 3-epimerases. In addition, the residues responsible for creating a hydrophobic environment around the substrate in TM0416p differ entirely from those in the other two enzymes. Collectively, these findings suggest that the substrate specificity of TM0416p is likely to differ substantially from those of other D-tagatose 3-epimerase family enzymes.
(キーワード): Bacterial Proteins / Carbohydrate Epimerases / Catalytic Domain / Crystallography, X-Ray / Hexoses / Protein Structure, Secondary / Structural Homology, Protein / Substrate Specificity / Thermotoga maritima
(出版サイトへのリンク): ● Publication site (DOI): 10.1107/S1744309109002115
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19255464; ● Search Scopus @ Elsevier (PMID): 19255464; ● Search Scopus @ Elsevier (DOI): 10.1107/S1744309109002115

(DOI: 10.1107/S1744309109002115, PubMed: 19255464) Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
A novel flavin adenine dinucleotide (FAD) containing d-lactate dehydrogenase from the thermoacidophilic crenarchaeota Sulfolobus tokodaii strain 7: purification, characterization and expression in Escherichia coli.,
Journal of Bioscience and Bioengineering, Vol.106, No.1, 16-21, 2008.

(要約): Dye-linked D-lactate dehydrogenase activity was found in the crude extract of a continental thermoacidophilic crenarchaeota, Sulfolobus tokodaii strain 7, and was purified 375-fold through four sequential chromatography steps. With a molecular mass of about 93 kDa, this enzyme was a homodimer comprised of identical subunits with molecular masses of about 48 kDa. The enzyme retained its full activity after incubation at 80 degrees C for 10 min and after incubation at pHs ranging from 6.5 to 10.0 for 30 min at 50 degrees C. The preferred substrate for this enzyme was D-lactate, with 2,6-dichloroindophenol serving as the electron acceptor. Using high-performance liquid chromatography (HPLC), the enzyme's prosthetic group was determined to be flavin adenine dinucleotide (FAD). Its N-terminal amino acid sequence was MLEGIEYSQGEEREDFVGFKIKPKI. Using that sequence and previously reported genome information, the gene encoding the enzyme (ST0649) was identified. It was subsequently cloned and expressed in Escherichia coli and found to encode a polypeptide of 440 amino acids with a calculated molecular weight of 49,715. The amino acid sequence of this dye-linked D-lactate dehydrogenase showed higher homology (39% identity) with that of a glycolate oxidase subunit homologue from Archaeoglobus fulgidus, but less similarity (32% identity) to D-lactate dehydrogenase from A. fulgidus. Taken together, our findings indicate that the dye-linked D-lactate dehydrogenase from S. tokodaii is a novel type of FAD containing D-lactate dehydrogenase.
(キーワード): Amino Acid Sequence / Enzyme Activation / Escherichia coli / Flavin-Adenine Dinucleotide / Lactate Dehydrogenases / Molecular Sequence Data / Protein Engineering / Species Specificity / Sulfolobus
(出版サイトへのリンク): ● Publication site (DOI): 10.1263/jbb.106.16
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18691525; ● Search Scopus @ Elsevier (PMID): 18691525; ● Search Scopus @ Elsevier (DOI): 10.1263/jbb.106.16

(DOI: 10.1263/jbb.106.16, PubMed: 18691525) Haruhiko Sakuraba, Kazunari Yoneda, Kumiko Yoshihara, Kyoko Satoh, Ryushi Kawakami, Yoshihiro Uto, Hideaki Tsuge, Katsuyuki Takahashi, Hitoshi Hori and Toshihisa Ohshima :
Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-D-ribose-5-phosphate aldolase.,
Applied and Environmental Microbiology, Vol.73, No.22, 7427-7434, 2007.

(要約): Genes encoding 2-deoxy-d-ribose-5-phosphate aldolase (DERA) homologues from two hyperthermophiles, the archaeon Pyrobaculum aerophilum and the bacterium Thermotoga maritima, were expressed individually in Escherichia coli, after which the structures and activities of the enzymes produced were characterized and compared with those of E. coli DERA. To our surprise, the two hyperthermophilic DERAs showed much greater catalysis of sequential aldol condensation using three acetaldehydes as substrates than the E. coli enzyme, even at a low temperature (25 degrees C), although both enzymes showed much less 2-deoxy-d-ribose-5-phosphate synthetic activity. Both the enzymes were highly resistant to high concentrations of acetaldehyde and retained about 50% of their initial activities after a 20-h exposure to 300 mM acetaldehyde at 25 degrees C, whereas the E. coli DERA was almost completely inactivated after a 2-h exposure under the same conditions. The structure of the P. aerophilum DERA was determined by X-ray crystallography to a resolution of 2.0 A. The main chain coordinate of the P. aerophilum enzyme monomer was quite similar to those of the T. maritima and E. coli enzymes, whose crystal structures have already been solved. However, the quaternary structure of the hyperthermophilic enzymes was totally different from that of the E. coli DERA. The areas of the subunit-subunit interface in the dimer of the hyperthermophilic enzymes are much larger than that of the E. coli enzyme. This promotes the formation of the unique dimeric structure and strengthens the hydrophobic intersubunit interactions. These structural features are considered responsible for the extremely high stability of the hyperthermophilic DERAs.
(キーワード): Acetaldehyde / Aldehyde-Lyases / Archaeal Proteins / Binding Sites / Catalysis / Enzyme Stability / Hot Temperature / Hydrogen-Ion Concentration / Magnetic Resonance Spectroscopy / Models, Molecular / 分子構造 (molecular structure) / Protein Structure, Secondary / Protein Structure, Tertiary / Pyrobaculum / Ribosemonophosphates / 構造活性相関 (structureactivity relationship) / Substrate Specificity / Thermotoga maritima
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AEM.01101-07
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17905878; ● Search Scopus @ Elsevier (PMID): 17905878; ● Search Scopus @ Elsevier (DOI): 10.1128/AEM.01101-07

(DOI: 10.1128/AEM.01101-07, PubMed: 17905878) Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Gene cloning and characterization of the very large NAD-dependent L-glutamate dehydrogenase from the psychrophile Janthinobacterium lividum, isolated from cold soil,
Journal of Bacteriology, Vol.189, No.15, 5626-5633, 2007.

(要約): NAD-dependent l-glutamate dehydrogenase (NAD-GDH) activity was detected in cell extract from the psychrophile Janthinobacterium lividum UTB1302, which was isolated from cold soil and purified to homogeneity. The native enzyme (1,065 kDa, determined by gel filtration) is a homohexamer composed of 170-kDa subunits (determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Consistent with these findings, gene cloning and sequencing enabled deduction of the amino acid sequence of the subunit, which proved to be comprised of 1,575 amino acids with a combined molecular mass of 169,360 Da. The enzyme from this psychrophile thus appears to belong to the GDH family characterized by very large subunits, like those expressed by Streptomyces clavuligerus and Pseudomonas aeruginosa (about 180 kDa). The entire amino acid sequence of the J. lividum enzyme showed about 40% identity with the sequences from S. clavuligerus and P. aeruginosa enzymes, but the central domains showed higher homology (about 65%). Within the central domain, the residues related to substrate and NAD binding were highly conserved, suggesting that this is the enzyme's catalytic domain. In the presence of NAD, but not in the presence of NADP, this GDH exclusively catalyzed the oxidative deamination of l-glutamate. The stereospecificity of the hydride transfer to NAD was pro-S, which is the same as that of the other known GDHs. Surprisingly, NAD-GDH activity was markedly enhanced by the addition of various amino acids, such as l-aspartate (1,735%) and l-arginine (936%), which strongly suggests that the N- and/or C-terminal domains play regulatory roles and are involved in the activation of the enzyme by these amino acids.
(キーワード): Amino Acid Sequence / Bacterial Proteins / Base Sequence / Binding Sites / Catalytic Domain / Chromatography, Gel / Cloning, Molecular / Conserved Sequence / DNA, Bacterial / Electrophoresis, Polyacrylamide Gel / Glutamate Dehydrogenase / Glutamic Acid / Molecular Sequence Data / Molecular Weight / NAD / NADP / Oxalobacteraceae / Protein Subunits / Pseudomonas aeruginosa / Sequence Analysis, DNA / Sequence Homology, Amino Acid / Soil Microbiology / Streptomyces / Substrate Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/JB.00496-07
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17526698; ● Search Scopus @ Elsevier (PMID): 17526698; ● Search Scopus @ Elsevier (DOI): 10.1128/JB.00496-07

(DOI: 10.1128/JB.00496-07, PubMed: 17526698) Toshihisa Ohshima, Ryushi Kawakami, Yuichiro Kanai, Shuichiro Goda and Haruhiko Sakuraba :
Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii.,
Protein Expression and Purification, Vol.54, No.1, 73-78, 2007.

(要約): 2-Keto-3-deoxygluconate kinase (KDGK) catalyzes the ATP-dependent phosphorylation of 2-keto-3-deoxygluconate, a key intermediate in the modified (semi-phosphorylative) Entner-Doudoroff (ED) glucose metabolic pathway. We identified the gene (ORF ID: ST2478) encoding KDGK in the hyperthermophilic archaeon Sulfolobus tokodaii based on the structure of a gene cluster in a genomic database and functionally expressed it in Escherichia coli. The expressed protein was purified from crude extract by heat treatment and two conventional column chromatography steps, and the partial amino acid sequence in the N-terminal region of the purified enzyme (MAKLIT) was identical to that obtained from the gene sequence. The purified enzyme was extremely thermostable and retained full activity after heating at 80 degrees C for 1 h. The enzyme utilized ATP or GTP, but not ADP or AMP, as a phosphoryl donor and 2-keto-3-deoxy-D-gluconate or 2-keto-D-gluconate as a phosphoryl acceptor. Divalent cations including Mg(2+), Co(2+), Ni(2+), Zn(2+) or Mn(2+) were required for activity, and the apparent Km values for KDG and ATP at 50 degrees C were 0.027 mM and 0.057 mM, respectively. The presence of KDGK means that the hyperthermophilic archaeon S. tokodaii metabolizes glucose via both modified (semi-phosphorylative) and non-phosphorylative ED pathways.
(キーワード): Archaeal Proteins / Cations, Divalent / Cloning, Molecular / 大腸菌 (Escherichia coli) / グルコース (glucose) / Hydrogen-Ion Concentration / Metals / リン酸化 (phosphorylation) / Phosphotransferases (Alcohol Group Acceptor) / Purine Nucleotides / Sulfolobus
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.pep.2007.02.013
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17407821; ● Search Scopus @ Elsevier (PMID): 17407821; ● Search Scopus @ Elsevier (DOI): 10.1016/j.pep.2007.02.013

(DOI: 10.1016/j.pep.2007.02.013, PubMed: 17407821) Yoneda Kazunari, Ryushi Kawakami, Tagashira Yuya, Haruhiko Sakuraba, Shuichiro Goda and Toshihisa Ohshima :
The first archaeal l-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus: gene cloning and enzymological characterization,
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol.1764, No.6, 1087-1093, 2006.

(要約): A gene encoding an L-aspartate dehydrogenase (EC 1.4.1.21) homologue was identified in the anaerobic hyperthermophilic archaeon Archaeoglobus fulgidus. After expression in Escherichia coli, the gene product was purified to homogeneity, yielding a homodimeric protein with a molecular mass of about 48 kDa. Characterization revealed the enzyme to be a highly thermostable L-aspartate dehydrogenase, showing little loss of activity following incubation for 1 h at up to 80 degrees C. The optimum temperature for L-aspartate dehydrogenation was about 80 degrees C. The enzyme specifically utilized L-aspartate as the electron donor, while either NAD or NADP could serve as the electron acceptor. The Km values for L-aspartate were 0.19 and 4.3 mM when NAD or NADP, respectively, served as the electron acceptor. The Km values for NAD and NADP were 0.11 and 0.32 mM, respectively. For reductive amination, the Km values for oxaloacetate, NADH and ammonia were 1.2, 0.014 and 167 mM, respectively. The enzyme showed pro-R (A-type) stereospecificity for hydrogen transfer from the C4 position of the nicotinamide moiety of NADH. This is the first report of an archaeal L-aspartate dehydrogenase. Within the archaeal domain, homologues of this enzyme occurred in many Methanogenic species, but not in Thermococcales or Sulfolobales species.
(キーワード): Amino Acid Oxidoreductases / Amino Acid Sequence / Archaeoglobus fulgidus / Cloning, Molecular / Dimerization / Enzyme Stability / Escherichia coli / Kinetics / Molecular Sequence Data / Oxaloacetate / Protein Structure, Tertiary / Sequence Homology, Amino Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbapap.2006.04.006
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16731057; ● Search Scopus @ Elsevier (PMID): 16731057; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbapap.2006.04.006

(DOI: 10.1016/j.bbapap.2006.04.006, PubMed: 16731057) Zheng Haitao, Hirose Yutaka, Kimura Tomokazu, Suye Shin-ichiro, Hori Teruo, Katayama Hideo, Arai3 Jun-ichiro, Ryushi Kawakami and Toshihisa Ohshima :
L-Proline sensor based on layer-by-layer immobilization of thermostable dye-linked L-proline dehydrogenase and polymerized mediator,
Science and Technology of Advanced Materials, Vol.7, No.3, 243-248, 2006.

(要約): L-Proline sensor has been fabricated through multilayer assembly of recombinant dye-linked L-proline dehydrogenase (L-proDH) originally from hyperthermophilic archaeon (Therococcus profundus) and polymerized mediator, poly(allylamine) ferrocene (PAA-Fc), on a gold electrode by electrostatic layer-by-layer alternate adsorption method. The characteristic redox peaks of ferrocene groups were obvious exhibited in cyclic voltammograms, and both anodic and cathodic peaks increased with PAA-Fc/L-proDH bilayer number which confirmed the formation of a multilayer structure. Further experiments showed that an electrode fabricated in this way catalyzed the oxidation of L-proline. It suggested that polymerized mediator could transfer electrons between electrode surface and immobilized L-proDH. Ampeormetric experiments showed that the current response to L-proline increased with PAA-Fc/L-proDH bilayer number. The stability of the sensor was measured, and it kept a 40% relative response after 30 days storage in buffer under 4 °C.
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.stam.2005.12.010
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1016/j.stam.2005.12.010

(DOI: 10.1016/j.stam.2005.12.010) Shuichiro Goda, Masaki Kojima, Yoshimi Nishikawa, Chizu Kujo, Ryushi Kawakami, Seiki Kuramitsu, Haruhiko Sakuraba, Yuzuru Hiragi and Toshihisa Ohshima :
Intersubunit Interaction Induced by Subunit Rearrangement Is Essential for the Catalytic Activity of the Hyperthermophilic Glutamate Dehydrogenase from Pyrobaculum islandicum.,
Biochemistry, Vol.44, No.46, 15304-15313, 2005.

(要約): The specific activity of recombinant Pyrobaculum islandicum glutamate dehydrogenase (pis-GDH) expressed in Escherichia coli is much lower than that of the native enzyme. However, when the recombinant enzyme is heated at 90 degrees C or exposed to 5 M urea, the activity increases to a level comparable to that of the native enzyme. Small-angle X-ray scattering measurements revealed that the radius of gyration (R(g,z)) of the hexameric recombinant enzyme was reduced to 47 A from 55 A by either heat or urea, and that the final structure of the active enzyme is the same irrespective of the mechanism of activation. Activation was accompanied by a shift in the peaks of the Kratky plot, though the molecular mass of the enzyme was unchanged. The activation-induced decline in R(g,z) followed first-order kinetics, indicating that activation of the enzyme involved a transition between two states, which was confirmed by singular-value decomposition analysis. When the low-resolution structure of the recombinant enzyme was restored using ab initio modeling, we found it to possess no point symmetry, whereas the heat-activated enzyme possessed 32-point symmetry. In addition, a marked increase in the fluorescence emission was observed with addition of ANS to the inactive recombinant enzyme but not the active forms, indicating that upon activation hydrophobic residues on the surface of the recombinant protein moved to the interior. Taken together, these data strongly suggest that subunit rearrangement, i.e., a change in the quaternary structure of the hexameric recombinant pis-GDH, is essential for activation of the enzyme.
(キーワード): Calorimetry, Differential Scanning / Cloning, Molecular / Enzyme Activation / Glutamate Dehydrogenase / Hot Temperature / Hydrophobic and Hydrophilic Interactions / Protein Structure, Quaternary / Pyrobaculum / Recombinant Proteins / Urea / X-Ray Diffraction
(出版サイトへのリンク): ● Publication site (DOI): 10.1021/bi050478l
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16285734; ● Search Scopus @ Elsevier (PMID): 16285734; ● Search Scopus @ Elsevier (DOI): 10.1021/bi050478l

(DOI: 10.1021/bi050478l, PubMed: 16285734) Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, Hideo Ago, Masashi Miyano, Kenji Aki, Nobuhiko Katunuma and Toshihisa Ohshima :
Crystal Structure of a Novel FAD-, FMN-, and ATP-containing L-Proline Dehydrogenase Complex from Pyrococcus horikoshii,
The Journal of Biological Chemistry, Vol.280, No.35, 31045-31049, 2005.

(要約): Two novel types of dye-linked L-proline dehydrogenase complex (PDH1 and PDH2) were found in a hyperthermophilic archaeon, Pyrococcus horikoshii OT3. Here we report the first crystal structure of PDH1, which is a heterooctameric complex (alphabeta)4 containing three different cofactors: FAD, FMN, and ATP. The structure was determined by x-ray crystallography to a resolution of 2.86 angstroms. The structure of the beta subunit, which is an L-proline dehydrogenase catalytic component containing FAD as a cofactor, was similar to that of monomeric sarcosine oxidase. On the other hand, the alpha subunit possessed a unique structure composed of a classical dinucleotide fold domain with ATP, a central domain, an N-terminal domain, and a Cys-clustered domain. Serving as a third cofactor, FMN was located at the interface between the alpha and beta subunits in a novel configuration. The observed structure suggests that FAD and FMN are incorporated into an electron transfer system, with electrons passing from the former to the latter. The function of ATP is unknown, but it may play a regulatory role. Although the structure of the alpha subunit differs from that of the beta subunit, except for the presence of an analogous dinucleotide domain with a different cofactor, the structural characteristics of PDH1 suggest that each represents a divergent enzyme that arose from a common ancestral flavoenzyme and that they eventually formed a complex to gain a new function. The structural characteristics described here reveal the PDH1 complex to be a unique diflavin dehydrogenase containing a novel electron transfer system.
(キーワード): Adenosine Triphosphate / Archaeal Proteins / Crystallography, X-Ray / Flavin Mononucleotide / Flavin-Adenine Dinucleotide / Models, Molecular / Multienzyme Complexes / Proline Oxidase / Protein Structure, Quaternary / Protein Subunits / Pyrococcus horikoshii
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.C500234200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16027125; ● Search Scopus @ Elsevier (PMID): 16027125; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.C500234200

(DOI: 10.1074/jbc.C500234200, PubMed: 16027125) Yasuhiro Shimizu, Haruhiko Sakuraba, Ryushi Kawakami, Shuichiro Goda, Yutaka Kawarabayasi and Toshihisa Ohshima :
L-Threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3, --- gene cloning and enzymatic characterization ---,
Extremophiles, Vol.9, No.4, 317-324, 2005.

(要約): A gene encoding the L-threonine dehydrogenase homologue has been identified in a hyperthermophlic archaeon Pyrococcus horikoshii OT3 via genome sequencing. The gene was cloned and expressed in Escherichia coli. The purified enzyme from the recombinant E. coli was extremely thermostable; the activity was not lost after incubation at 100 degrees C for 20 min. The enzyme (molecular mass: 192 kDa) is composed of a tetrameric structure with a type of subunit (41 kDa). The enzyme is specific for NAD and utilizes L-threonine, L-serine and DL-threo-3-phenylserine as the substrate. The enzyme required divalent cations such as Zn(2+), Mn(2+) and Co(2+) for the activity, and contained one zinc ion/subunit. The K(m) values for L-threonine and NAD at 50 degrees C were 0.20 mM and 0.024 mM, respectively. Kinetic analyses indicated that the L-threonine oxidation reaction proceeds via a random mechanism with regard to the binding of L-threonine and NAD. The enzyme showed pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of NADH. This is the first description of the characteristics of an L-threonine dehydrogenase from the archaea domain.
(キーワード): Alcohol Oxidoreductases / Amino Acid Sequence / Animals / Catalysis / Cloning, Molecular / Hydrogen / Magnetic Resonance Spectroscopy / Molecular Sequence Data / NADP / Pyrococcus horikoshii / Recombinant Proteins / Sequence Alignment / Sequence Homology, Amino Acid / Serine / Stereoisomerism / Substrate Specificity
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00792-005-0447-2
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15902509; ● CiNii @ 国立情報学研究所 (CRID): 1571417126449425536; ● Search Scopus @ Elsevier (PMID): 15902509; ● Search Scopus @ Elsevier (DOI): 10.1007/s00792-005-0447-2

(DOI: 10.1007/s00792-005-0447-2, PubMed: 15902509, CiNii: 1571417126449425536) Ryushi Kawakami, Haruhiko Sakuraba, Hideaki Tsuge, Shuichiro Goda, Nobuhiko Katunuma and Toshihisa Ohshima :
A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3,
The FEBS Journal, Vol.272, No.16, 4044-4054, 2005.

(要約): Two distinguishable activity bands for dye-linked l-proline dehydrogenase (PDH1 and PDH2) were detected when crude extract of the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 was run on a polyacrylamide gel. After purification, PDH1 was found to be composed of two different subunits with molecular masses of 56 and 43 kDa, whereas PDH2 was composed of four different subunits with molecular masses of 52, 46, 20 and 8 kDa. The native molecular masses of PDH1 and PDH2 were 440 and 101 kDa, respectively, indicating that PDH1 has an alpha4beta4 structure, while PDH2 has an alphabetagammadelta structure. PDH2 was found to be similar to the dye-linked l-proline dehydrogenase complex from Thermococcus profundus, but PDH1 is a different type of enzyme. After production of the enzyme in Escherichia coli, high-performance liquid chromatography showed the PDH1 complex to contain the flavins FMN and FAD as well as ATP. Gene expression and biochemical analyses of each subunit revealed that the beta subunit bound FAD and exhibited proline dehydrogenase activity, while the alpha subunit bound ATP, but unlike the corresponding subunit in the T. profundus enzyme, it exhibited neither proline dehydrogenase nor NADH dehydrogenase activity. FMN was not bound to either subunit, suggesting it is situated at the interface between the alpha and beta subunits. A comparison of the amino-acid sequences showed that the ADP-binding motif in the alpha subunit of PDH1 clearly differs from that in the alpha subunit of PDH2. It thus appears that a second novel dye-linked l-proline dehydrogenase complex is produced in P. horikoshii.
(キーワード): Amino Acid Sequence / Chromatography, High Pressure Liquid / Coloring Agents / Genes, Archaeal / Molecular Sequence Data / Proline Oxidase / Pyrococcus horikoshii / Recombinant Proteins / Sequence Homology, Amino Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1742-4658.2005.04810.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16098188; ● Search Scopus @ Elsevier (PMID): 16098188; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1742-4658.2005.04810.x

(DOI: 10.1111/j.1742-4658.2005.04810.x, PubMed: 16098188) Haruhiko Sakuraba, Ryushi Kawakami and Toshihisa Ohshima :
First Archaeal Inorganic Polyphosphate/ATP-Dependent NAD Kinase, from Hyperthermophilic Archaeon Pyrococcus horikoshii, --- Cloning, Expression, and Characterization ---,
Applied and Environmental Microbiology, Vol.71, No.8, 4352-4358, 2005.

(要約): The gene (PH1074) encoding the NAD kinase of the hyperthermophilic archaeon Pyrococcus horikoshii was identified in the genome database, cloned, and functionally expressed in Escherichia coli. The recombinant enzyme was purified to homogeneity by heat treatment at 90 degrees C for 20 min and one successive HiTrap affinity chromatography step. The purified enzyme was easily precipitated by dialysis against phosphate buffer without NaCl and imidazole and was usually stored in buffer containing 0.5 M NaCl and 0.5 M imidazole to avoid precipitation. The molecular mass of the active enzyme was determined to be 145 kDa by a gel filtration method, and the enzyme was composed of a tetramer of 37-kDa subunits. The archaeal enzyme utilized several nucleoside triphosphates, such as GTP, CTP, UTP, and ITP, as well as ATP and inorganic polyphosphates [poly(P)] as phosphoryl donors for NAD phosphorylation. The enzyme utilized poly(P)27 (the average length of the phosphoryl chain was 27) as the most active inorganic polyphosphate for NAD phosphorylation. Thus, this enzyme is categorized as an inorganic polyphosphate/ATP-dependent NAD kinase. The enzyme was the most thermostable NAD kinase found to date: its activity was not lost by incubation at 95 degrees C for 10 min. The enzyme showed classical Michaelis-Menten-type kinetics for NAD and ATP, but not for poly(P)27. The Km values for NAD were determined to be 0.30 and 0.40 mM when poly(P)27 and ATP, respectively, were used as the phosphoryl donors. The Km value for ATP was 0.29 mM, and the concentration of poly(P)27 which gave half of the maximum enzyme activity was 0.59 mM. The enzyme required several metal cations, such as Mg2+, Mn2+, or Ni2+, for its activity. The deduced amino acid sequence showed a low level of identity to those of E. coli ATP-dependent NAD kinase (31%) and the inorganic polyphosphate/ATP-dependent NAD kinase of Mycobacterium tuberculosis (29%). This is the first description of the characteristics of a poly(P)/ATP-dependent NAD kinase from a hyperthermophilic archaeon.
(キーワード): Adenosine Triphosphate / Amino Acid Sequence / Cloning, Molecular / Enzyme Stability / Escherichia coli / Hot Temperature / Hydrogen-Ion Concentration / Kinetics / Molecular Sequence Data / Phosphates / Phosphotransferases (Alcohol Group Acceptor) / Polyphosphates / Pyrococcus horikoshii / Sequence Alignment / Temperature
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AEM.71.8.4352-4358.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16085824; ● Search Scopus @ Elsevier (PMID): 16085824; ● Search Scopus @ Elsevier (DOI): 10.1128/AEM.71.8.4352-4358.2005

(DOI: 10.1128/AEM.71.8.4352-4358.2005, PubMed: 16085824) Ryushi Kawakami, Haruhiko Sakuraba, Shintaro Kamohara, Shuichiro Goda, Yutaka Kawarabayasi and Toshihisa Ohshima :
Oxidative stress response in an anaerobic hyperthermophilic archaeon, --- presence of a functional peroxiredoxin in Pyrococcus horikoshii ---,
The Journal of Biochemistry, Vol.136, No.4, 541-547, 2004.

(要約): 嫌気性超好熱古細菌Pyrococcus horikoshiiに僅かな酸素ストレスを与えると，顕著に誘導されるタンパク質を見出し，これがペルオキシレドキシンであることを明らかにした．嫌気性菌が酸素ストレス応答タンパク質を持っており，これが菌体の酸素耐性に関与する可能性を示した．
(キーワード): Amino Acid Sequence / Archaeal Proteins / Blotting, Northern / Cloning, Molecular / Culture Media / Databases, Genetic / Dithiothreitol / Electrons / Electrophoresis, Gel, Two-Dimensional / Electrophoresis, Polyacrylamide Gel / Escherichia coli / Genome / Hot Temperature / Humans / Hydrogen-Ion Concentration / Models, Chemical / Molecular Sequence Data / Oxidative Stress / Oxygen / Peroxidases / Peroxiredoxins / Protein Structure, Tertiary / Pyrococcus horikoshii / RNA / Recombinant Proteins / Sequence Homology, Amino Acid / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jb/mvh157
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15625325; ● Search Scopus @ Elsevier (PMID): 15625325; ● Search Scopus @ Elsevier (DOI): 10.1093/jb/mvh157

(DOI: 10.1093/jb/mvh157, PubMed: 15625325) Haruhiko Sakuraba, Ryushi Kawakami, Hajime Takahashi and Toshihisa Ohshima :
Novel Archaeal Alanine:Glyoxylate Aminotransferase from Thermococcus litoralis,
Journal of Bacteriology, Vol.186, No.16, 5513-5518, 2004.

(要約): 超好熱菌に初めてアラニングリオキシル酸アミノトランスフェラーゼを見出した．一次構造解析により系統樹を作製した結果，既知のアラニングリオキシル酸アミノトランスフェラーゼとは全く異なるグループに属することが判明した．本酵素は，芳香族アミノ酸トランスフェラーゼに近縁であるが，反応はアラニンとグリオキシル酸に特異的であった．本菌が本酵素やグリオキシル酸レダクターゼを含むグリオキシル酸代謝経路を持つ事を提唱した．
(キーワード): Amino Acid Sequence / Chromatography / DNA, Archaeal / Electrophoresis, Polyacrylamide Gel / Molecular Sequence Data / Phylogeny / Sequence Analysis, DNA / Sequence Analysis, Protein / Sequence Homology / Thermococcus / Transaminases
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/JB.186.16.5513-5518.2004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15292154; ● Search Scopus @ Elsevier (PMID): 15292154; ● Search Scopus @ Elsevier (DOI): 10.1128/JB.186.16.5513-5518.2004

(DOI: 10.1128/JB.186.16.5513-5518.2004, PubMed: 15292154) Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex,
Extremophiles, Vol.8, No.2, 99-108, 2004.

(要約): 超好熱アーキアThermococcus profundusに見出した新規色素依存性L-プロリン脱水素酵素の遺伝子クローニングに成功し，本酵素がユニークなヘテロテトラマー構造をとること，これらをコードする4種の遺伝子がオペロンを形成することを明らかにした．また，この異種サブユニットの共発現系の構築に成功した．本成果および関連研究により，第5回酵素応用シンポジウム研究奨励賞受賞．平成16年度医科学応用研究財団奨励金受賞．成果は日経バイオテク2004/6/21, pp19-20に掲載された．
(キーワード): Amino Acid Sequence / Base Sequence / Coloring Agents / Hot Temperature / Macromolecular Substances / Magnetic Resonance Spectroscopy / Molecular Sequence Data / Molecular Weight / Multigene Family / Proline Oxidase / Protein Conformation / Protein Subunits / Sequence Alignment / Sequence Homology, Amino Acid / Thermococcus
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00792-003-0368-x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15064976; ● Search Scopus @ Elsevier (PMID): 15064976; ● Search Scopus @ Elsevier (DOI): 10.1007/s00792-003-0368-x

(DOI: 10.1007/s00792-003-0368-x, PubMed: 15064976) Haruhiko Sakuraba, Hideaki Tsuge, Ikuko Shimoya, Ryushi Kawakami, Shuichiro Goda, Yutaka Kawarabayasi, Nobuhiko Katunuma, Hideo Ago, Masashi Miyano and Toshihisa Ohshima :
The First Crystal Structure of Archaeal Aldolase: Unique Tetrameric Structure of 2-Deoxy-D-ribose-5-phosphate Aldolase from Hyperthermophilic Archaeon Aeropyrum pernix,
The Journal of Biological Chemistry, Vol.278, No.12, 10799-10806, 2003.

(要約): 古細菌のアルドラーゼに関して，初めてX線結晶構造解析に成功した．解析したデオキシリボース5-リン酸アルドラーゼは抗がん剤や抗ウイルス剤の合成中間体の生産に利用できるが，既知の酵素は不安定で産業への実用化が難しい．本酵素は非常に安定性が高く，実用面での有用性を示した．また，その安定性が本酵素のユニークな4次構造に由来することを提唱した．本研究により，平成15年度バイオインダストリー協会「グラント」奨励金受賞．
(キーワード): Amino Acid Sequence / Archaea / Archaeal Proteins / Enzyme Stability / Escherichia coli / Fructose-Bisphosphate Aldolase / Hydrogen-Ion Concentration / Molecular Sequence Data / Protein Subunits / Recombinant Proteins / Ribosemonophosphates
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M212449200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12529358; ● Search Scopus @ Elsevier (PMID): 12529358; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M212449200

(DOI: 10.1074/jbc.M212449200, PubMed: 12529358) Haruhiko Sakuraba, Issei Yoshioka, Shinji Koga, Mamoru Takahashi, Yuki Kitahama, Takenori Satomura, Ryushi Kawakami and Toshihisa Ohshima :
ADP-Dependent Glucokinase/Phosphofructokinase, a Novel Bifunctional Enzyme from the Hyperthermophilic Archaeon Methanococcus jannaschii,
The Journal of Biological Chemistry, Vol.277, No.15, 12495-12498, 2002.

(要約): 超好熱アーキアMethanococcus jannashiiに見出したグルコキナーゼおよびホスホフルクトキナーゼの両活性を持つ新規酵素について報告している．この酵素がグルコキナーゼおよびホスホフルクトキナーゼの起源であることを提唱した．本酵素の発見により，メタン菌による糖からのメタン生産が理論上可能であることを示した．生化学分野のトップジャーナルの1つであるJ. Biol. Chem.において，特に重要な発見を紹介するAccelerated Publicationに掲載された．本研究により平成14年度ビタミン学会奨励賞受賞．
(キーワード): Amino Acid Sequence / Base Sequence / Catalysis / DNA Primers / Glucokinase / Methanococcus / Molecular Sequence Data / Phosphorylation / Phosphotransferases (Alcohol Group Acceptor) / Phylogeny / Recombinant Proteins / Sequence Homology, Amino Acid
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.C200059200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11856730; ● CiNii @ 国立情報学研究所 (CRID): 1360574094658110464; ● Search Scopus @ Elsevier (PMID): 11856730; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.C200059200

(DOI: 10.1074/jbc.C200059200, PubMed: 11856730, CiNii: 1360574094658110464) Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Dye-linked D-Proline Dehydrogenase from Hyperthermophilic Archaeon Pyrobaculum islandicum Is a Novel FAD-dependent Amino Acid Dehydrogenase,
The Journal of Biological Chemistry, Vol.277, No.15, 12861-12867, 2002.

(要約): 超好熱アーキアPyrobaculum islandicumに初めて見出した新規色素依存性D-プロリン脱水素酵素に関して報告している．本酵素は従来報告されているD-アミノ酸脱水素酵素とは大きく構造や性質が異なることを明らかにした．D-アミノ酸の合成およびD-プロリンの定量用バイオセンサー素子としての有用性が期待できる．
(キーワード): Amino Acid Sequence / Base Sequence / Chromatography, Ion Exchange / Coloring Agents / DNA Probes / Electrophoresis, Polyacrylamide Gel / Enzyme Stability / Molecular Sequence Data / Proline Oxidase / Sequence Homology, Amino Acid / Thermoproteaceae
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M112272200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11823469; ● CiNii @ 国立情報学研究所 (CRID): 1364233269761772032; ● Search Scopus @ Elsevier (PMID): 11823469; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M112272200

(DOI: 10.1074/jbc.M112272200, PubMed: 11823469, CiNii: 1364233269761772032) Haruhiko Sakuraba, Takenori Satomura, Ryushi Kawakami, Sanae Yamamoto, Yutaka Kawarabayasi, Hisasi Kikuchi and Toshihisa Ohshima :
L-Aspartate oxidase is present in the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii OT-3: characteristics and role in the de novo biosynthesis of nicotinamide adenine dinucleotide proposed by genome sequencing,
Extremophiles, Vol.6, No.4, 275-281, 2002.

(要約): 超好熱アーキアPyrococcus horikoshiiに嫌気性菌で初めて見出したL-アスパラギン酸オキシダーゼについて報告している．本酵素が嫌気条件下でも機能する色素依存性L-アスパラギン酸脱水素酵素反応も触媒することを見出し，NAD生合成系の初発酵素として機能していることを提唱した．本酵素に特徴的な脱水素酵素反応によるL-アスパラギン酸定量への応用が期待できる．本研究により平成14年度ビタミン学会奨励賞受賞．
(キーワード): Amino Acid Oxidoreductases / Amino Acid Sequence / Cloning, Molecular / Enzyme Stability / Genome, Archaeal / Hydrogen-Ion Concentration / Kinetics / Molecular Sequence Data / NAD / Pyrococcus / Recombinant Proteins / Substrate Specificity / Temperature
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00792-001-0254-3
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12215812; ● Search Scopus @ Elsevier (PMID): 12215812; ● Search Scopus @ Elsevier (DOI): 10.1007/s00792-001-0254-3

(DOI: 10.1007/s00792-001-0254-3, PubMed: 12215812) Haruhiko Sakuraba, Yoshinori Takamatsu, Takenori Satomura, Ryushi Kawakami and Toshihisa Ohshima :
Purification, Characterization, and Application of a Novel Dye-Linked L-Proline Dehydrogenase from a Hyperthermophilic Archaeon, Thermococcus profundus,
Applied and Environmental Microbiology, Vol.67, No.4, 1470-1475, 2001.

(要約): 超好熱アーキアThermococcus profundusに見出した新規色素依存性L-プロリン脱水素酵素について報告している．本酵素は新規色素依存性酵素の中では非常に安定性が高いことを明らかにした．果物やハチミツの品質管理マーカーであるL-プロリンの定量用バイオセンサー素子としての有用性を明らかにした．
(キーワード): 2,6-Dichloroindophenol / Amino Acid Sequence / Coloring Agents / Molecular Sequence Data / Proline / Proline Oxidase / Substrate Specificity / Thermococcus
(出版サイトへのリンク): ● Publication site (DOI): 10.1128/AEM.67.4.1470-1475.2001
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11282592; ● Search Scopus @ Elsevier (PMID): 11282592; ● Search Scopus @ Elsevier (DOI): 10.1128/AEM.67.4.1470-1475.2001

(DOI: 10.1128/AEM.67.4.1470-1475.2001, PubMed: 11282592)

MISC

研究者総覧に該当データはありませんでした。

総説・解説

櫻庭春彦, 郷田秀一郎, 川上竜巳, 大島敏久, 津下英明, 勝沼信彦 :
超好熱菌タンパク質の耐熱化の分子戦略,
化学と生物, Vol.44, No.5, 305-312, 2006年5月.

(出版サイトへのリンク): ● Publication site (DOI): 10.1271/kagakutoseibutsu1962.44.305
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1571417125313316352; ● Search Scopus @ Elsevier (DOI): 10.1271/kagakutoseibutsu1962.44.305

(DOI: 10.1271/kagakutoseibutsu1962.44.305, CiNii: 1571417125313316352) 津下英明, 川上竜巳, 櫻庭春彦, 安藝謙嗣, 勝沼信彦, 大島敏久 :
新規ジフラビン脱水素酵素ファミリー : FAD, FMN, ATP結合L-プロリン脱水素酵素複合体の構造と機能,
生化学, Vol.78, No.1, 41-45, 2006年1月.

(キーワード): Adenosine Triphosphate / Crystallization / Flavin Mononucleotide / Flavin-Adenine Dinucleotide / Proline Oxidase / Protein Conformation / Pyrococcus horikoshii / Sarcosine Oxidase
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16491912; ● CiNii @ 国立情報学研究所 (CRID): 1520853832706586496; ● Search Scopus @ Elsevier (PMID): 16491912

(PubMed: 16491912, CiNii: 1520853832706586496) 川上竜巳, 櫻庭春彦, 大島敏久 :
超好熱アーキアの新規フラビン含有アミノ酸脱水素酵素の機能開発,
酵素工学ニュース, Vol.54, 31-36, 2005年10月.

講演・発表

Fukuda J., Yoneda K., Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Crystallization and Chracterization of L-lysine Dehydrogenase (LysDH) from Hyperthermophilic Archaeon yrococcus horikoshii,
The first joint Symposium on life between Pusan National Univeristy and Kyushu University, Fukuoka, Feb. 2007. Yoneda K., Ryushi Kawakami, Tagashira Y., Haruhiko Sakuraba, Shuichiro Goda, Hideaki Tsuge, Katunuma N. and Toshihisa Ohshima :
The first crystal structure of an archaeal L-aspartate dehydrogenase from the hyperthermophile Archaeoglobus fulgidus,
EXTREMOPHILES 2006 CONFERENCE, Brest, Sep. 2006. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, Kenji Aki, Nobuhiko Katunuma, Hideo Ago, Masashi Miyano and Toshihisa Ohshima :
The Crystal Structure of L-proline Dehydrogenase in a Hyperthermophilic Archaeon,
XX Congress of the International Union of Crystallography, Vol.27, No.50, C198, Florence, Italy, Aug. 2005.

(キーワード): dehydrogenase / flavins / complex structure

Toshihisa Ohshima, Ryushi Kawakami and Haruhiko Sakuraba :
Gene and primary stuructures, and properties of a novel type of dye-linked FAD-containing L-proline dehydrogenase complex from the hyperthremophilic archaeon Thermococcus profundus,
The eighth China-Japan-Korea joint symposium on enzyme engineering, 43-44, Hangzhou, China, Oct. 2004.

(要約): 超好熱アーキアThermococcus profundusに見出した新規色素依存性L-プロリン脱水素酵素の遺伝子クローニングに成功し，本酵素がユニークなヘテロテトラマー構造をとること，これらをコードする4種の遺伝子がオペロンを形成することを明らかにした．また，この異種サブユニットの共発現系の構築に成功した．

Shin-ichiro Suye, Yutaka Hirose, Tomokazu Kimura, Haitao Zheng, Teruo Hori, Hideo Katayama, Ryushi Kawakami and Toshihisa Ohshima :
Amperometric DNA sensor using gold electrode modified with polymeric mediator and thermostable dehydrogenase by layer-by-layer adsorption,
Nano and Giga Challenges in Microelectronics Symposium and Summer School Reserch and Development Opportunities, Cracow, Sep. 2004. Hideaki Tsuge, Ryushi Kawakami, Haruhiko Sakuraba, Kazunari Yoneda, Hideo Ago, Masashi Miyano, Kenji Aki, Nobuhiko Katunuma and Toshihisa Ohshima :
Crystal structure of an unique FAD and FMN containing enzyme; Dye-linked L-proline dehydrogenase from Pyrococcus horikoshii,
The 1st Pacific-Rim International Conference on Protein Science, Yokohama, Apr. 2004.

(要約): 超好熱アーキアPyrococcus horikoshiiに見出した新規色素依存性L-プロリン脱水素酵素の遺伝子クローニングに成功し，これらをコードする2種の遺伝子の共発現系構築に成功した．さらに本酵素のX線結晶構造解析に成功した．

Ryushi Kawakami, Hideaki Tsuge, Shuichiro Goda, Haruhiko Sakuraba, Nobuhiko Katunuma and Toshihisa Ohshima :
Novel dye-linked L-proline dehydrogenase from anaerobic hyperthermophilic archaea, Thermococcus profundus and Pyrococcus horikoshii OT-3,
The 1st Pacific-Rim International Conference on Protein Science, Yokohama, Apr. 2004.

(要約): 超好熱アーキアThermococcus profundusに見出した新規色素依存性L-プロリン脱水素酵素の遺伝子クローニングに成功し，本酵素がユニークなヘテロテトラマー構造をとることを明らかにした．

Shuichiro Goda, Haruhiko Sakuraba, Mohammad W Bhuiya, Ryushi Kawakami, Seiki Kuramitsu, Yuzuru Hiragi and Toshihisa Ohshima :
Heat and Urea Induced Activations by Subunit Rearrangement of Hyperthermophilic Glutamate Dehydrogenase Produced in Escherichia Coli,
Thermophiles 2003, Vol.P2.17, 143, Exeter, United Kingom, Sep. 2003.

(要約): 大腸菌内で不活性型として生産された超好熱菌由来グルタミン酸脱水素酵素酵素の熱および尿素による成熟化機構について熱力学的解析を行った．

Ryushi Kawakami, Toshihisa Ohshima and Haruhiko Sakuraba :
Novel dye-linked L-prorine dehydrogenase from a hyperthemophilic archaeon, Thermococcus profundus, --- Subunit structure, gene structure and catalytic properties ---,
9th International Symposium on the Genetics of Industrial Microorganisms, Gyeongju, Korea, Jul. 2002.

(要約): 超好熱アーキアThermococcus profundusに見出した新規色素依存性L-プロリン脱水素酵素の遺伝子クローニングに成功し，本酵素がユニークなヘテロテトラマー構造をとること，これらをコードする4種の遺伝子がオペロンを形成することを明らかにした．

Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba and Toshihisa Ohshima :
Purification and characterization of dye-linked D-proline dehydrogenase from hyperthermophilic archaeon Pyrobaculum islandicum,
International Congress on Biocatalysis, Hamburg, Germany, Jun. 2002.

(要約): 超好熱アーキアPyrobaculum islandicumに初めて見出した新規色素依存性D-プロリン脱水素酵素に関して報告している．本酵素は従来報告されているD-アミノ酸脱水素酵素とは大きく構造や性質が異なることを明らかにした．

Toshihisa Ohshima, Haruhiko Sakuraba, Takenori Satomura and Ryushi Kawakami :
Two novel dye-linked proline dehydrogenases from anaerobic hyperthermophilic archaea: Their function, structure and application,
Japan-Italy Symposium New Trends in Enzyme Science and Technology, Kyoto, Nov. 2001.

(要約): 超好熱アーキアに見出した2種類の新規色素依存性L-プロリン脱水素酵素の遺伝子クローニングに成功し，これらをコードする遺伝子がオペロンを形成することを明らかにした．

服部武文, 片山恵, 川上竜巳, 林順司, 山村正臣 :
マツタケ由来S-アデノシル-L-メチオニン依存ケイ皮酸カルボキシルメチルトランスフェラーゼ組換え酵素の特性解明,
第74回日本木材学会大会(京都大会), 2024年3月. 川上竜巳, 林順司, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus furiosusのPLP依存性アミノ酸ラセマーゼの機能と構造の特徴,
2023年度日本ビタミン学会大会, 2023年6月. 山﨑義輝, 山下晶央, 佐々木千鶴, 林順司, 川上竜巳, 金丸芳 :
鳴門産スジアオノリ(Ulva prolifera)の志賀毒素吸着活性,
日本農芸化学会中四国支部第64 回講演会要旨集, 2023年1月.

(キーワード): E. coli O157 / Siga Toxin / Ulva prolifera / adsorption

川上竜巳, 河瀬智紀, 上原太良, 櫻庭春彦, 大島敏久 :
PH0140転写因子によるアミノ酸ラセマーゼ遺伝子クラスターの発現制御,
2022年度極限環境生物学会, 2022年11月. 山下晶央, 山﨑義輝, 佐々木千鶴, 林順司, 川上竜巳, 金丸芳 :
アラメ(Eisenia bicyclis)によるEHEC O157 産生志賀毒素吸着,
日本農芸化学会中四国支部大会講演要旨集, 37, 2022年9月.

(キーワード): E. coli O157 / Siga Toxin / Eisenia bicyclis / adsorption

田中保, Md Hanif Ali, 小林美佑, Rumana Yesmin Hasi, 粟飯原睦美, 林順司, 川上竜巳 :
極長鎖脂肪酸による毒性とその解毒装置としてのペルオキシソームの役割,
脂質栄養学, Vol.31, No.2, 143, 2022年9月. 中永美樹, 川上竜巳, 林順司, 大島敏久, 櫻庭春彦 :
超好熱性アーキア Thermococcus profundus 由来色素依存性 L -プロリン脱水素酵素に関する研究,
日本農芸化学会2022年度中四国支部大会 2022年9月22日, 2022年9月. 林順司, 玉谷優奈, 小川詩緒里, 川上竜巳, 里村武範, 大島敏久, 櫻庭春彦 :
高度耐熱性FAD依存性D-乳酸脱水素酵素のX線結晶構造解析,
日本農芸化学会2022年度中四国支部大会(第63回講演会), 2022年9月. Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Distribution and characterization of glycosylinositol phosphoceramide specific phospholipase D in Brassica plants,
第64回日本脂質生化学会, Vol.64, 272-275, Jun. 2022. Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Peroxisomes attenuate lipotoxicity of very-long-chain fatty acids,
第64回日本脂質生化学会, Vol.64, 43-46, Jun. 2022. 林順司, 大志田達也, 川上竜巳, 里村武範, 若山守, 大島敏久, 櫻庭春彦 :
超好熱アーキア由来色素依存性D-乳酸脱水素酵素の構造解析,
日本ビタミン学会第74回大会, 2022年6月. Hanif Ali, Miyu Kobayashi, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Metabolism and biological effect of very-long-chain fatty acid in peroxisome-deficient cells,
第63回日本生化学中国・四国支部例会, May 2022. 川上竜巳, 河瀬智紀, 佐藤樹夫, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshiiにおけるL-Ile/D-allo-Ileによるアミノ酸ラセマーゼ遺伝子の発現制御,
2021年度極限環境生物学会, 2021年11月. Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Characterization of uptake and metabolism of very-long-chain fatty acid in peroxisome-deficient CHO cells,
第94回日本生化学大会, Nov. 2021. Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Production of phytoceramide 1- phosphate and inositol glycan by glycosylinositol phosphoceramide specific phospholipase D activity in plants,
第94回日本生化学大会, Nov. 2021. 櫻庭春彦, 大志田達也, 川上竜巳, 林順司, 米田一成, 大島敏久 :
好熱アーキアPyrococcus horikoshii 由来オルニチンアミノトランスフェラーゼの構造解析,
第465回ビタミンB研究協議会, 2021年11月. Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya, Kazunori Sango and Tamotsu Tanaka :
Characterization of uptake and metabolism of very-long-chain fatty acid in peroxisome-deficient CHO cells,
第62回日本生化学中国・四国支部例会, Sep. 2021. Rumana Yesmin Hasi, Naohiro Imura, Toshiki Ishikawa, Hiroyuki Imai, Yoshimichi Takai, Hanif Ali, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru and Tamotsu Tanaka :
Production of phytoceramide 1- phosphate and inositol glycan by glycosylinositol phosphoceramide specific phospholipase D activity in plants,
第62回日本生化学中国・四国支部例会, Sep. 2021. Hanif Ali, Katsuya Morito, Rumana Yesmin Hasi, Mutsumi Aihara, Junji Hayashi, Ryushi Kawakami, Kaori Kanemaru, Koichiro Tsuchiya and Tamotsu Tanaka :
Uptake and metabolism of very-long-chain fatty acid in animal cells,
第63回日本脂質生化学会, Jun. 2021. 林順司, 大志田達也, 川上竜巳, 里村武範, 若山守, 大島敏久, 櫻庭春彦 :
超好熱菌由来色素依存性D-乳酸脱水素酵素のX線結晶構造解析,
日本農芸化学会中四国支部第58回講演会, 2021年1月. 川上竜巳, 林順司, 木下千夏, 河瀬智紀, 佐藤樹夫 :
超好熱アーキア Thermococcus litoralis DSM5473 のアミノ酸ラセマーゼ BAR2 の機能解析,
日本ビタミン学会第 72 回大会, 2020年6月. 田中保, Rumana Hasi Yesmin, 森戸克弥, 小暮健太朗, 林順司, 川上竜巳, 金丸芳, 今井博之, 石川寿樹 :
グリコシルイノシトールホスホセラミドの単離法の開発,
第12回セラミド研究会学術集会, 2019年10月. 田中保, 森戸克弥, Rumana Hasi Yesmin, 林順司, 川上竜巳, 金丸芳, 若山睦, 近藤千恵子, 福田達也, 小暮健太朗 :
食品素材に含まれるセラミドの簡便な定量方法,
日本脂質栄養学会第28回大会, 2019年9月. 川上竜巳, 半澤七菜, 大志田達也, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT-3のオルニチンアミノトランスフェラーゼの機能解析と構造解析,
2019年度日本ビタミン会大会, 2019年6月. 川上竜巳 :
超好熱アーキアのFAD依存性脱水素酵素複合体及びPLP依存性アミノ酸ラセマーゼの研究,
2019年度日本ビタミン会大会奨励賞受賞講演, 2019年6月. 川上竜巳, 金丸芳, 横井川久己男 :
食品微生物や発酵食品に含まれるD-アミノ酸検出のためのUHPLC検出系の構築,
第38回日本日本食品微生物学会学術総会, 2017年10月. Badr Ali Hoida, Takahashi Keiko, Ryushi Kawakami, Yasuo Oyama, Kumio Yokoigawa and Kaori Kanemaru :
Screening and analysis of edible seaweeds in the ability to adsorb Shiga toxin,
第38回日本日本食品微生物学会学術総会, Oct. 2017. 川上竜巳, 藤原拓, 金茂里沙, 辻奈々子 :
超好熱アーキアPyrococcus horikoshii OT-3のアミノ酸ラセマーゼホモログの酵素化学的解析,
日本農芸化学会中四国支部第48回講演会, 2017年6月. 大志田達也, 林順司, 里村武範, 川上竜巳, 大島敏久, 櫻庭春彦 :
高度好塩菌 Haloarcula japonica 由来 2-デオキシリボース 5-リン酸アルドラーゼに関する研究,
日本農芸化学会2017年度大会, 2017年3月. 金丸芳, 生杉笙, 川上竜巳, 小山保夫, 横井川久己男 :
徳島県産食用植物のガン細胞増殖抑制,
日本農芸化学会2017年度(平成29年度)大会講演要旨集, 2017年3月. 川上竜巳, 藤原拓, 金茂里沙, 辻奈々子 :
超好熱アーキアPyrococcus horikoshii OT-3のD-アミノ酸培養とアミノ酸ラセマーゼ活性の相関,
2016年度極限環境生物学会, 2016年11月. 大志田達也, 林順司, 里村武範, 川上竜巳, 大島敏久, 櫻庭春彦 :
高度好塩菌 Haloarcula japonica由来2-デオキシリボース-5-リン酸アルドラーゼに関する研究,
日本農芸化学会2016年度中四国支部大会, 2016年9月. 川上竜巳, 櫻庭春彦, 大森勇門, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT3のアミノ酸ラセマーゼの酵素化学的解析,
日本農芸化学会中四国支部第45回講演会, 2016年6月. 川上竜巳 :
超好熱アーキアのD-アミノ酸利用とアミノ酸ラセマーゼの発見,
日本農芸化学会中四国支部第22回若手シンポジウム, 2016年4月. 川上竜巳 :
超好熱アーキアのL-プロリン脱水素酵素:機能・構造・応用,
日本農芸化学会中四国支部第18回若手シンポジウム, 2014年9月. 川上竜巳, 山下久美, 大森勇門, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT-3 由来アミノ酸ラセマーゼの同定,
2014年度日本ビタミン会大会, 2014年6月. 川上竜巳, 櫻庭春彦, 大島敏久 :
L-プロリン脱水素酵素からのオキシダーゼ活性除去の検討,
2013年度日本生物工学会大会, 2013年9月. 黒田早織, 川上竜巳, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshiiのNAD合成酵素(NADS)の機能解析,
日本農芸化学会2007年度大会, 2007年3月. 川上竜巳, 櫻庭春彦, 大島敏久 :
低温菌由来の巨大グルタミン酸脱水素酵素,
酵素工学研究会, 2006年10月. 大島敏久, 里村武範, 川上竜巳, 櫻庭春彦, 谷裕児, 薮谷智規, 本仲純子 :
超好熱アーキアPyrobaculum islandicumの色素依存性D-プロリン脱水素酵素の機能解析と応用,
第2回D-アミノ酸研究学術講演会, 2006年9月. 川上竜巳, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
低温菌Janthinobacterium lividum UTB1302が生産するNAD依存性グルタミン酸脱水素酵素の遺伝子クローニング,
日本ビタミン学会第58回大会, 2006年5月. 吉行貴司, 伊藤佳洋, 川上竜巳, 郷田秀一郎, 櫻庭春彦, 櫻庭春彦, 大島敏久 :
超好熱アーキアSulfolobus tokodaii strain7 由来2-keto-3-deoxygluconate (KDG) aldolase の機能解析,
日本農芸化学会2006年度大会, 2006年3月. 寺井利光, 広瀬由鷹, 末信一郎, 堀照夫, 川上竜巳, 櫻庭春彦, 大島敏久 :
耐熱性L-プロリン脱水素酵素の電極上への分子配向制御固定,
日本農芸化学会2006年度大会, 2006年3月. 川上竜巳, 櫻庭春彦, 津下英明, 勝沼信彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshiiの新規色素依存性L-プロリン脱水素酵素の機能と構造,
日本Archaea研究会第18回講演会, 2005年7月. 川上竜巳, 田頭祐哉, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
超好熱アーキアArchaeoglobus fulgidusのNAD生合成に機能すると予想されるL-AspDHホモログの機能解析,
日本ビタミン学会第57回大会, 2005年5月. 大島敏久, 本多恭子, 川上竜巳, 櫻庭春彦, 新居修 :
スクモ藍建て工程に関与するインジゴ還元酵素の精製と性質,
日本農芸化学会2005年度大会, 2005年3月. 櫻庭春彦, 川上竜巳, 津下英明, 郷田秀一郎, 勝沼信彦, 安藝謙嗣, 大島敏久 :
超好熱アーキアPyrococcus horikoshiiに存在する新規ATP含有アミノ酸脱水素酵素,
日本農芸化学会2005年度大会, 2005年3月. 廣瀬由鷹, 木村友和, 鄭海涛, 末信一朗, 堀照夫, 片山秀夫, 川上竜巳, 大島敏久 :
リコンビナント耐熱性L-プロリン脱水素酵素を用いたLegionella属菌のDNA検出システム,
日本農芸化学会2005年度大会, 2005年3月. 津下英明, 川上竜巳, 櫻庭春彦, 吾郷日出夫, 宮野雅司, 安藝謙嗣, 勝沼信彦, 大島敏久 :
P. horikoshii由来L-プロリン脱水素酵素のX線結晶構造解析,
日本生物物理学会第42回年会, 2004年12月. 大島敏久, 川上竜巳, 櫻庭春彦, 津下英明 :
超好熱アーキアの色素依存性脱水素酵素の探索とその応用,
第27回日本分子生物学会年会, 369, 2004年12月. 廣瀬由鷹, 木村友和, 鄭海涛, 末信一朗, 堀照夫, 片山秀夫, 川上竜巳, 大島敏久 :
超好熱アーキア由来色素依存性L-プロリン脱水素酵素を用いたLegionella属菌検出DNAセンサーの構築,
日本生物工学会平成16年度大会, 240, 2004年9月. Haitao Zheng, Yukata Hirose, Tomokazu Kimura, Shin-ichiro Suye, Teruo Hori, Hideo Katayama, Ryushi Kawakami and Toshihisa Ohshima :
Multilayer assembly of dye-linked L-proline dehydrogenase and poly(allylamine)ferrocene by layer-by layer adsorption method,
日本生物工学会平成16年度大会, 240, Sep. 2004. 川上竜巳, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
低温菌Janthinobacterium lividum UTB1302由来のグルタミン酸脱水素酵素の特徴,
日本生物工学会平成16年度大会, 109, 2004年9月. 川上竜巳, 津下英明, 郷田秀一郎, 櫻庭春彦, 勝沼信彦, 安藝謙嗣, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT-3から発見されたフラビン酵素，色素依存性L-プロリン脱水素酵素の機能と構造解析,
日本ビタミン学会第56回大会, Vol.78, No.4, 253, 2004年5月. 櫻庭春彦, 川上竜巳, 大島敏久 :
超好熱アーキアThermococcus litoralisに存在する新規アラニン:グリオキシル酸アミノトランスフェラーゼ,
日本ビタミン学会第56回大会, Vol.78, No.4, 246, 2004年5月. 本多恭子, 川上竜巳, 郷田秀一郎, 櫻庭春彦, 新居修, 大島敏久 :
藍染め染色液中の好アルカリ性菌の分離とインジゴ還元酵素の諸性質の解明,
日本農芸化学会2004年度大会, 263, 2004年3月. 川上竜巳, 郷田秀一郎, 櫻庭春彦, 津下英明, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT-3の色素依存性L-プロリン脱水素酵素の性質と構造,
日本農芸化学会2004年度大会, 118, 2004年3月. 郷田秀一郎, Koji Ono, 川上竜巳, 櫻庭春彦, 河原林裕, 大島敏久 :
Pyrococcus horikoshii agmatinase: gene expression in E. coli and characterization,
第76回日本生化学会大会, Vol.75, No.8, 1066, 2003年10月. 川上竜巳, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT-3に存在する2種類の色素依存性L-プロリン脱水素酵素の特徴,
日本生物工学会平成15年度大会, 45, 2003年9月. 郷田秀一郎, 櫻庭春彦, Mohammad W Bhuiya, 里村武範, 川上竜巳, 倉光成紀, 柊弓絃, 大島敏久 :
大腸菌によって発現された低活性超好熱菌由来グルタミン酸脱水素酵素の熱及び尿素による活性化過程のX線小角散乱と熱量計による解析,
日本アーキア研究会第16回講演会, 26, 2003年6月. 川上竜巳, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
超好熱アーキアThermococcus profundusの色素依存性L-プロリン/NADH脱水素酵素複合体の解析,
日本農芸化学会2003年度大会, 258, 2003年4月. 郷田秀一郎, 櫻庭春彦, Mohammad W Bhuiya, 里村武範, 川上竜巳, 柊弓絃, 大島敏久 :
好熱菌由来グルタミン酸脱水素酵素の熱及び尿素による成熟化課程の物理化学的解明,
日本農芸化学会2003年度大会, 241, 2003年4月. 里村武範, 川上竜巳, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
超好熱アーキアD-アミノ酸脱水素酵素の性質,
第3回極限環境微生物学会年会, 5-6, 2002年12月. 里村武範, 川上竜巳, 藤崎礼子, 中山奈央子, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
好熱好酸性アーキアSulfolobus tokodaii strain 7 由来 D- 乳酸脱水素酵素の特徴,
日本生物工学会平成14年度大会, 2002年10月. 川上竜巳, 里村武範, 郷田秀一郎, 櫻庭春彦, 大島敏久 :
超好熱アーキアPyrococcus horikoshii OT3 の色素依存性 L- プロリン脱水素酵素の精製と酵素遺伝子の同定,
日本生物工学会平成14年度大会, 2002年10月. 大島敏久, 川上竜巳, 櫻庭春彦 :
超好熱アーキアの色素依存性L-プロリン脱水素酵素の特徴,
第388回ビタミンB研究委員会, 2002年8月.

研究会・報告書: 大島敏久, 櫻庭春彦, 川上竜巳, 津下英明 :
超好熱菌のアミノ酸代謝関連酵素，NAD生合成系酵素の立体構造解析,
タンパク3000プロジェクト代謝系評価会, 2006年11月.

特許: 大島敏久, 櫻庭春彦, 川上竜巳, 吉田洋一, 下家郁子 : 耐熱性2-デオキシリボース-5-リン酸アルドラーゼ, 特願2002-049172 (2002年2月), 特開2003-250553 (2003年9月), .
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 超好熱アーキアに備わるＤ－アミノ酸を介した遺伝子発現機構と生理的意義 (研究課題/領域番号: 24K08666 )
担子菌特異的カルボキシルメチルトランスフェラーゼの反応機作の解明 (研究課題/領域番号: 23K05337 )
遺伝子発現とアミノ酸動態の解析によるＤ－アミノ酸誘導性タンパク質発現の解明 (研究課題/領域番号: 20K05729 )
超好熱アーキアのL‐プロリン脱水素酵素複合体の機能構造解析と電子伝達機構の解明 (研究課題/領域番号: 21780099 )
研究者番号（90380120）による検索

その他: 研究者総覧に該当データはありませんでした。

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2024年4月26日更新

専門分野・研究分野: 酵素化学 (Enzyme Chemistry)
所属学会・所属協会: 日本生物工学会
社団法人日本農芸化学会
日本ビタミン学会
極限環境生物学会
委員歴・役員歴: 日本生物工学会 (正会員 [2004年4月〜2024年3月])
社団法人日本農芸化学会 (中四国支部参与 [2017年4月〜2024年3月])
日本ビタミン学会 (正会員 [2010年4月〜2024年3月])
極限環境生物学会 (正会員 [2018年4月〜2024年3月])
日本生物工学会 (西日本支部編集委員 [2015年5月〜2017年5月])
日本ビタミン学会 (将来構想検討委員会 [2020年10月〜2024年3月])
社団法人日本農芸化学会 (正会員 [2017年4月〜2024年3月])
受賞: 2013年1月, 支部奨励賞 (日本農芸化学会西日本支部)
2019年6月, 日本ビタミン学会奨励賞 (日本ビタミン学会)
活動: 学生委員会委員 (2014年4月〜2016年3月)
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学生委員会委員 (2016年4月〜2021年3月)
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クラス副担任(2年生) (2020年4月〜2021年3月)
クラス副担任(1年生) (2020年4月〜2021年3月)
クラス担任(1年生) (2020年4月〜2021年3月)
クラス担任(2年生) (2020年4月〜2021年3月)
クラス担任(3年生) (2020年4月〜2021年3月)
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学生委員会委員 (2022年4月〜2024年3月)
インターンシップ委員会 (2023年4月〜2025年3月)

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Ｊグローバル

Jグローバル最終確認日: 2024/4/27 01:19
氏名（漢字）: 川上竜巳
氏名（フリガナ）: カワカミリュウシ
氏名（英字）: Kawakami Ryushi
所属機関: 徳島大学大学院社会産業理工学研究部准教授

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researchmap最終確認日: 2024/4/21 01:30
氏名（漢字）: 川上竜巳
氏名（フリガナ）: カワカミリュウシ
氏名（英字）: Kawakami Ryushi
プロフィール: 超好熱アーキアなどの極限環境微生物を対象としてD-アミノ酸などの代謝に関する酵素の研究を行っています。
登録日時: 2009/9/28 00:00
更新日時: 2024/2/1 18:00
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所属: 徳島大学大学院社会産業理工学研究部
部署: 生物資源産業学域
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2024年4月27日更新

研究者番号: 90380120

所属（現在）: 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授

所属（過去の研究課題 情報に基づく）*注記: 2020/4/1 – 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授
2009/4/1 – 2010/4/1 : 佐賀大学, 総合分析実験センター, 助教

審査区分/研究分野

研究代表者

生物系 / 農学 / 農芸化学 / 応用生物化学
小区分37020:生物分子化学関連
小区分38020:応用微生物学関連

研究代表者以外

小区分40020:木質科学関連

キーワード

研究代表者

酵素化学 / 構造生物学 / 生化学 / 超好熱アーキア / L-プロリン脱水素酵素複合体 / 鉄イオウクラスター / Thermococcus profundus / Pyrococcus horikoshii / 遺伝子発現 / D-アミノ酸 / 転写因子 / アミノ酸ラセマーゼ

研究代表者以外

ケイ皮酸メチル / Tricholoma matsutake / カルボキシルメチルトランスフェラーゼ

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2020年9月18日

生物の代謝酵素を利用した環境に優しい次世代地盤改良技術「バイオグラウト」の開発

平田章松尾義則真壁和裕 W. 渡部稔田中保金丸芳川上竜巳林順司

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川上竜巳