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井上美穂

徳島大学

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2025年4月25日更新

職名: 助教
電話: 研究者総覧に該当データはありませんでした。
電子メール: inoue.miho@tokushima-u.ac.jp
学歴: 研究者総覧に該当データはありませんでした。
学位: 博士(学術) (岡山大学)
職歴・経歴: 2016/4: 徳島大学助教, 大学院医歯薬学研究部

専門分野・研究分野: 骨ミネラル代謝学 (Bone and Mineral Metabolism)
生体材料学 (Biomaterials)
歯科補綴学 (Prosthodontics)

研究者総覧で最新データを確認する

2025年4月25日更新

専門分野・研究分野: 骨ミネラル代謝学 (Bone and Mineral Metabolism)
生体材料学 (Biomaterials)
歯科補綴学 (Prosthodontics)
担当経験のある授業科目: クラウンブリッジ補綴学 (学部)
クラウン補綴学実習 (学部)
ブリッジ補綴学実習 (学部)
口腔生理学 (学部)
統合医療実験・実習 (大学院)
統合医療演習 (大学院)
補綴系歯科学 (学部)
顎口腔機能再建学 (学部)
指導経験: 3人 (博士)

研究者総覧で最新データを確認する

2025年4月25日更新

専門分野・研究分野: 骨ミネラル代謝学 (Bone and Mineral Metabolism)
生体材料学 (Biomaterials)
歯科補綴学 (Prosthodontics)

研究テーマ: 抗Dickkopf1 (DKK-1) 抗体による骨分化能への影響と骨粗しょう症に対する骨分化能メカニズムの解明 (抗DKK-1抗体, 骨分化, 骨粗しょう症)
Tumor necrosis factor (TNF)-α処理炭酸アパタイトおよびハイドロキシアパタイトが骨形成におよぼす影響 (炭酸アパタイト, (tumor necrosis factor-α), 骨形成)
全部床義歯作製後の満足度と咀嚼能率の評価 (全部床義歯, 咀嚼能率, 満足度)

著書

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論文

Maki Hosoki, Mayu Miyagi, Kazuo Okura, Miho Inoue, Masamitsu Ohshima, Yoshitaka Suzuki, Aya Ozawa, Akari Shibagaki, Taniwaki Tatsuya, Kouhei Kamoi, Noriyuki Bando, Yasutomo Yoshihara, Mizuki Shinkai, Toyoko Tajima, Jaime Moreno Fabillar and Yoshizo Matsuka :
Proposals and evaluations for the enhancement of CAD/CAM education,
Journal of Prosthodontic Research, Vol.69, No.2, 133-135, 2025.

(出版サイトへのリンク): ● Publication site (DOI): 10.2186/jpr.JPR_D_24_00132
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 39443134; ● Search Scopus @ Elsevier (PMID): 39443134; ● Search Scopus @ Elsevier (DOI): 10.2186/jpr.JPR_D_24_00132

(DOI: 10.2186/jpr.JPR_D_24_00132, PubMed: 39443134) Toyoko Tajima, Maki Hosoki, Mayu Miyagi, Miho Inoue, A Ozawa, M Shinkai, Mio Naritani, Yoshiaki Kubo, S Raman, Parimal Chavan, Kazuyuki Koike and Yoshizo Matsuka :
Correlation between pierced earrings and metal allergy prevalence in Tokushima University hospital: A 15-year retrospective analysis,
Scientific Reports, Vol.15, No.1, 10939, 2025.

(要約): In Japan, metal allergies are becoming increasingly prevalent, raising concerns for public health. This study examined metal allergy characteristics, patient histories, and clinical signs associated with patch test results over a 15-year period at the Dental Metal Allergy Clinic of Tokushima University Hospital. A retrospective analysis of 1085 patients revealed that 65.4% tested positive for at least one metal allergen, with palladium chloride, nickel sulfate, potassium dichromate, and cobalt chloride identified as the most common allergens. Female patients were disproportionately affected, accounting for 78.4% of the study population. Notably, there was a substantial increase in patients reporting inflammation due to pierced earrings, increasing from 5.0% in 2005 to 43.2% in 2020, particularly among females. Patients with a history of inflammation from earrings had an 81.3% prevalence of metal allergies, which was significantly higher than the 60.4% reported in those without such a history (chi-square test, p < 0.001). These findings suggest a strong link between earrings and metal allergies, underscoring the need for improved education, early detection, and preventive strategies to address the growing impact of metal allergies on public health.
(キーワード): Humans / Female / Male / Retrospective Studies / Japan / Adult / Prevalence / Middle Aged / Hospitals, University / Nickel / Allergens / Adolescent / Hypersensitivity / Young Adult / Metals / Aged / Cobalt / Jewelry / Potassium Dichromate / Child / Patch Tests / Dermatitis, Allergic Contact / Palladium
(徳島大学機関リポジトリ): ● Metadata: 2013180
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-025-86868-1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 40157960; ● Search Scopus @ Elsevier (PMID): 40157960; ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-025-86868-1

(徳島大学機関リポジトリ: 2013180, DOI: 10.1038/s41598-025-86868-1, PubMed: 40157960) 井上美穂, 鈴木善貴, 山田輝乃, 井上正久, 松香芳三 :
睡眠時ブラキシズムに対するアロマテラピーの効果 ―無作為化二重盲検クロスオーバー比較試験―,
アロマテラピー学雑誌, Vol.26, No.1, 24-30, 2025年.

(キーワード): アロマテラピー / 睡眠時ブラキシズム / ウェアラブル筋電計 / ベルガモット / マージョラム
(出版サイトへのリンク): ● Publication site (DOI): 10.15035/aeaj.260103
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390584586126972416; ● Search Scopus @ Elsevier (DOI): 10.15035/aeaj.260103

(DOI: 10.15035/aeaj.260103, CiNii: 1390584586126972416) Yoshitaka Suzuki, Kazuo Okura, Toyoko Tajima, Junhel Dalanon, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Mayu Miyagi, Daisuke Ikutame and Yoshizo Matsuka :
Essence of diagnosis and management of sleep bruxism,
Journal of Oral Health and Biosciences, Vol.36, No.2, 22-29, 2024.

(キーワード): Sleep Bruxism / Occlusal appliance / Diagnosis / Management
(徳島大学機関リポジトリ): ● Metadata: 2011998
(出版サイトへのリンク): ● Publication site (DOI): 10.20738/johb.36.2_22
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390862555421219200; ● Search Scopus @ Elsevier (DOI): 10.20738/johb.36.2_22

(徳島大学機関リポジトリ: 2011998, DOI: 10.20738/johb.36.2_22, CiNii: 1390862555421219200) Huijiao Yan, Masamitsu Ohshima, Raju Raju, S Raman, Kazumitsu Sekine, Arief Waskitho, Miho Inoue, Masahisa Inoue, Otto Baba, Tsuyoshi Morita, Mayu Miyagi and Yoshizo Matsuka :
Dentin-pulp complex tissue regeneration via three-dimensional cell sheet layering,
Tissue Engineering. Part C, Methods, Vol.27, No.10, 559-570, 2021.

(要約): The dentin-pulp complex is a unique structure in teeth that contains both hard and soft tissues. Generally, deep caries and trauma cause damage to the dentin-pulp complex, and if left untreated, this damage will progress to irreversible pulpitis. The aim of this study was to fabricate a layered cell sheet composed of rat dental pulp (DP) cells and odontogenic differentiation of pulp (OD) cells and to investigate the ability to regenerate the dentin-pulp complex in a scaffold tooth. We fabricated two single cell sheets composed of DP cells (DP cell sheet) or OD cells (OD cell sheet) and a layered cell sheet made by layering both cells. The characteristics of the fabricated cell sheets were analyzed using light microscopy, scanning electron microscope (SEM), hematoxylin-eosin (HE) staining, and immunohistochemistry (IHC). Furthermore, the cell sheets were transplanted into the subrenal capsule of immunocompromised mice for 8 weeks. After this, the regenerative capacity to form dentin-like tissue was evaluated using micro-computed tomography (micro-CT), HE staining, and IHC. The findings of SEM and IHC confirmed that layered cell sheets fabricated by stacking OD cells and DP cells maintained their cytological characteristics. Micro-CT of layered cell sheet transplants revealed a mineralized capping of the access cavity in the crown area, similar to that of natural dentin. In contrast, the OD cell sheet group demonstrated the formation of irregular fragments of mineralized tissue in the pulp cavity, and the DP cell sheet did not develop any hard tissue. Moreover, bone volume/tissue volume (BV/TV) showed a significant increase in hard tissue formation in the layered cell sheet group compared with that in the single cell sheet group ( < 0.05). HE staining also showed a combination of soft and hard tissue formation in the layered cell sheet group. Furthermore, IHC confirmed that the dentin-like tissue generated from the layered cell sheet expressed characteristic markers of dentin but not bone equivalent to that of a natural tooth. In conclusion, this study demonstrates the feasibility of regenerating dentin-pulp complex using a bioengineered tissue designed to simulate the anatomical structure. Impact statement The dentin-pulp complex can be destroyed by deep caries and trauma, which may cause pulpitis and progress to irreversible pulpitis, apical periodontitis, and even tooth loss. Current treatments cannot maintain pulp health, and teeth can become brittle. We developed a three-dimensional (3D) layered cell sheet using dental pulp cells and odontogenic differentiation of pulp cells for dentin-pulp complex regeneration. Our layered cell sheet enables the regeneration of an organized 3D dentin-pulp-like structure comparable with that of natural teeth. This layered cell sheet technology may contribute to dentin-pulp complex regeneration and provide a novel method for complex tissue engineering.
(徳島大学機関リポジトリ): ● Metadata: 2009904
(出版サイトへのリンク): ● Publication site (DOI): 10.1089/ten.TEC.2021.0171
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34583551; ● Search Scopus @ Elsevier (PMID): 34583551; ● Search Scopus @ Elsevier (DOI): 10.1089/ten.TEC.2021.0171

(徳島大学機関リポジトリ: 2009904, DOI: 10.1089/ten.TEC.2021.0171, PubMed: 34583551) Rika Hayama, Kazuo Okura, Masamitsu Ohshima, Maki Hosoki, Yoshitaka Suzuki, Mayu Miyagi, Miho Inoue, Takuma Iwasa, Junhel Dalanon, Raju Resmi, Omar Maningo Rodis and Yoshizo Matsuka :
Longitudinal comparison between flipped classroom and team-based learning in a prosthodontic class,
Journal of Oral Health and Biosciences, Vol.34, No.1, 1-10, 2021.

(要約): Active learning is a concept that allows students to study and learn actively by themselves to get knowledge. There are several methods of active learning, including flipped classroom (FC) and team-based learning (TBL). In FC, students are required to study before classes. In TBL, students also study before class, take individual readiness assurance test (iRAT) and team readiness assurance test (tRAT), then discuss group assignment projects (GAPs) during class. The purpose of this study was to compare the effectiveness between FC and TBL.<br> The effectiveness of FC and TBL was assessed from the results of the term-end examinations, questionnaires and practice examinations. To check the difficulty of the term-end examinations, control dentists took the same examinations and we calculated the equating score with item response theory.<br> Statistical analysis showed that the correct answer rate in term-end examinations was significantly different in comparison with the time of the trial, and for the participants (students and dentists). The term-end examination score of FC and TBL did not show a statistical difference. The student questionnaire showed that TBL had higher scores than FC on various factors such as student positive attitude, preparation, ingenuity of teacher and achieving the class goals. The crown & bridge score from the practice examination of 6<sup>th</sup>-year students who had FC + TBL were constantly higher than the Japanese national average score. The identification index of FC and TBL did not show the statistical difference and there was no statistical difference on item response theory between FC and TBL.
(キーワード): Flipped classroom / Team-based learning / Prosthodontics
(徳島大学機関リポジトリ): ● Metadata: 2009204
(出版サイトへのリンク): ● Publication site (DOI): 10.20738/johb.34.1_1
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390852259674142080; ● Search Scopus @ Elsevier (DOI): 10.20738/johb.34.1_1

(徳島大学機関リポジトリ: 2009204, DOI: 10.20738/johb.34.1_1, CiNii: 1390852259674142080) Hiroo Kawahara, Miho Inoue, Kazuo Okura, Masamitsu Ohshima and Yoshizo Matsuka :
Risk factors for tooth loss in patients with 25 remaining teeth undergoing mid-long-term,
International Journal of Environmental Research and Public Health, Vol.18, No.13, 7174, 2021.

(徳島大学機関リポジトリ): ● Metadata: 2009058
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijerph18137174
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85109198478

(徳島大学機関リポジトリ: 2009058, DOI: 10.3390/ijerph18137174, Elsevier: Scopus) 松香芳三, 大島正充, 大倉一夫, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂 :
インドネシアの歯学部における学部教育,
日本口腔リハビリテーション学会雑誌, Vol.33, No.1, 53-57, 2020年.

(キーワード): Undergraduate student education / dental school / Indonesia
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520572359516377600

(CiNii: 1520572359516377600) H Kawahara, Miho Inoue, Kazuo Okura, Masamitsu Ohshima and Yoshizo Matsuka :
Risk factors for tooth loss in patients undergoing mid long-term maintenance: A retrospective study,
International Journal of Environmental Research and Public Health, Vol.17, No.17, E6258, 2020.

(要約): In this retrospective study, we identified risk factors for tooth loss in patients undergoing mid-long-term maintenance therapy. We surveyed 674 maintenance patients for ≥5 years after active treatment who visited a dental clinic between January 2015 and December 2016. Of these, 265 were men (mean age 54.6 ± 8.0 years old) and 409 were women (mean age 54.0 ± 7.9 years old). Study variables included patient compliance, sex, number of teeth lost, cause of tooth loss (dental caries, periodontal disease, root fracture, others, vital or non-vital teeth), age at start of maintenance, number of remaining teeth at start of maintenance, smoking, use of salivary secretion inhibitors, presence of diabetes mellitus, condition of periodontal bone loss, and use of a removable denture. Most lost teeth were non-vital teeth (91.7% of all cases) and the most common cause of tooth loss was tooth fracture (62.1% of all cases). A statistically significant risk factors for tooth loss was number of remaining teeth at the start of maintenance ( = 0.003).
(徳島大学機関リポジトリ): ● Metadata: 2008099
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijerph17176258
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32867387; ● Search Scopus @ Elsevier (PMID): 32867387; ● Search Scopus @ Elsevier (DOI): 10.3390/ijerph17176258

(徳島大学機関リポジトリ: 2008099, DOI: 10.3390/ijerph17176258, PubMed: 32867387) Resmi Raju, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Arief Waskitho, Otto Baba, Masahisa Inoue and Yoshizo Matsuka :
Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet,
Scientific Reports, Vol.10, No.1, 1656, 2020.

(要約): Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
(徳島大学機関リポジトリ): ● Metadata: 2007179
(出版サイトへのリンク): ● Publication site (DOI): 10.1038/s41598-020-58222-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 32015383; ● Search Scopus @ Elsevier (PMID): 32015383; ● Search Scopus @ Elsevier (DOI): 10.1038/s41598-020-58222-0

(徳島大学機関リポジトリ: 2007179, DOI: 10.1038/s41598-020-58222-0, PubMed: 32015383) Takuma Iwasa, S Afroz, Miho Inoue, Rieko Arakaki, Masamitsu Ohshima, R Raju, Arief Waskitho, Inoue Masahisa, Otto Baba and Yoshizo Matsuka :
IL-10 and CXCL2 in trigeminal ganglia in neuropathic pain,
Neuroscience Letters, Vol.703, 132-138, 2019.

(徳島大学機関リポジトリ): ● Metadata: 2006707
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.neulet.2019.03.031
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85063442797

(徳島大学機関リポジトリ: 2006707, DOI: 10.1016/j.neulet.2019.03.031, Elsevier: Scopus) Mio Naritani, Miho Inoue, Resmi Raju, Mayu Miyagi, Masamitsu Ohshima and Yoshizo Matsuka :
Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α),
Journal of Hard Tissue Biology, Vol.28, No.2, 99-108, 2019.

(徳島大学機関リポジトリ): ● Metadata: 2006466
(出版サイトへのリンク): ● Publication site (DOI): 10.2485/jhtb.28.99
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85065527448

(徳島大学機関リポジトリ: 2006466, DOI: 10.2485/jhtb.28.99, Elsevier: Scopus) S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka :
CGRP induces differential regulation of cytokines from satellite glial cells in trigeminal ganglia and orofacial nociception,
International Journal of Molecular Sciences, Vol.20, No.3, 711, 2019.

(要約): Neuron-glia interactions contribute to pain initiation and sustainment. Intra-ganglionic (IG) secretion of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) modulates pain transmission through neuron-glia signaling, contributing to various orofacial pain conditions. The present study aimed to investigate the role of satellite glial cells (SGC) in TG in causing cytokine-related orofacial nociception in response to IG administration of CGRP. For that purpose, CGRP alone (10 μL of 10 M), Minocycline (5 μL containing 10 μg) followed by CGRP with one hour gap (Min + CGRP) were administered directly inside the TG in independent experiments. Rats were evaluated for thermal hyperalgesia at 6 and 24 h post-injection using an operant orofacial pain assessment device (OPAD) at three temperatures (37, 45 and 10 °C). Quantitative real-time PCR was performed to evaluate the mRNA expression of IL-1β, IL-6, TNF-α, IL-1 receptor antagonist (IL-1RA), sodium channel 1.7 (NaV 1.7, for assessment of neuronal activation) and glial fibrillary acidic protein (GFAP, a marker of glial activation). The cytokines released in culture media from purified glial cells were evaluated using antibody cytokine array. IG CGRP caused heat hyperalgesia between 6-24 h (paired- test, < 0.05). Between 1 to 6 h the mRNA and protein expressions of GFAP was increased in parallel with an increase in the mRNA expression of pro-inflammatory cytokines IL-1β and anti-inflammatory cytokine IL-1RA and NaV1.7 (one-way ANOVA followed by Dunnett's post hoc test, < 0.05). To investigate whether glial inhibition is useful to prevent nociception symptoms, Minocycline (glial inhibitor) was administered IG 1 h before CGRP injection. Minocycline reversed CGRP-induced thermal nociception, glial activity, and down-regulated IL-1β and IL-6 cytokines significantly at 6 h (-test, < 0.05). Purified glial cells in culture showed an increase in release of 20 cytokines after stimulation with CGRP. Our findings demonstrate that SGCs in the sensory ganglia contribute to the occurrence of pain via cytokine expression and that glial inhibition can effectively control the development of nociception.
(徳島大学機関リポジトリ): ● Metadata: 2006708
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms20030711
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 30736422; ● Summary page in Scopus @ Elsevier: 2-s2.0-85061270594

(徳島大学機関リポジトリ: 2006708, DOI: 10.3390/ijms20030711, PubMed: 30736422, Elsevier: Scopus) Miho Inoue, Mio Naritani, R Raju, Mayu Miyagi, Masamitsu Ohshima, Inoue Masahisa and Yoshizo Matsuka :
Effect of short-term Tumour Necrosis Factor-alpha (TNF-α) -stimulation on the growth and differentiation of MC3T3-E1 osteoblast-like cells,
Journal of Hard Tissue Biology, Vol.27, No.3, 213-218, 2018.

(徳島大学機関リポジトリ): ● Metadata: 2006458
(出版サイトへのリンク): ● Publication site (DOI): 10.2485/jhtb.27.213
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85049948297

(徳島大学機関リポジトリ: 2006458, DOI: 10.2485/jhtb.27.213, Elsevier: Scopus) Keisuke Nishigawa, Rika Hayama, Ktsushi Omoto, Kazuo Okura, Toyoko Tajima, Yoshitaka Suzuki, Maki Hosoki, Mayu Miyagi, Miho Inoue, Omar Maningo Rodis and Yoshizo Matsuka :
Validity of peer evaluation for team-based learning in a dental school in Japan,
Journal of Dental Education, Vol.81, No.12, 1451-1456, 2017.

(要約): The aim of this study was to determine the validity of peer evaluation for team-based learning (TBL) classes in dental education in comparison with the term-end examination records and TBL class scores. Examination and TBL class records of 256 third- and fourth-year dental students in six fixed prosthodontics courses from 2013 to 2015 in one dental school in Japan were investigated. Results of the term-end examination during those courses, individual readiness assurance test (IRAT), group readiness assurance test (GRAT), group assignment projects (GAP), and peer evaluation of group members in TBL classes were collected. Significant positive correlations were found between all combinations of peer evaluation, IRAT, and term-end examination. Individual scores also showed a positive correlation with group score (total of GRAT and GAP). From the investigation of the correlations in the six courses, significant positive correlations between peer evaluation and individual score were found in four of the six courses. In this study, peer evaluation seemed to be a valid index for learning performance in TBL classes. To verify the effectiveness of peer evaluation, all students have to realize the significance of scoring the team member's performance. Clear criteria and detailed instruction for appropriate evaluation are also required.
(キーワード): Education, Dental / Educational Measurement / Group Processes / Humans / Japan / Learning / Peer Group / Reproducibility of Results / Schools, Dental / Teaching
(徳島大学機関リポジトリ): ● Metadata: 2006075
(出版サイトへのリンク): ● Publication site (DOI): 10.21815/JDE.017.106
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 29196333; ● Summary page in Scopus @ Elsevier: 2-s2.0-85038129509

(徳島大学機関リポジトリ: 2006075, DOI: 10.21815/JDE.017.106, PubMed: 29196333, Elsevier: Scopus) Miho Inoue, KAWAHARA Hiroo, Kazuo Okura, Mayu Miyagi, NARITANI Mio, Takuma Iwasa, OMOTO Katsuhiro, Omar Maningo Rodis and Yoshizo Matsuka :
Frequency of congenitally missing permanent teeth at a primary dental care clinic in Tokushima, Japan,
The Journal of Japan Association of Oral Rehabilitation, Vol.30, No.1, 67-70, 2017.

(キーワード): Congenitally missing permanent teeth / hypodontia / primary dental care
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1520290884130282880

(CiNii: 1520290884130282880) Shintaro Sukegawa, Hitoshi Higuchi, Miho Inoue, Hitoshi Nagatsuka, Shigeru Maeda and Takuya Miyawaki :
Locally injected dexmedetomidine inhibits carrageenin-induced inflammatory responses in the injected region,
Anesthesia & Analgesia, Vol.118, No.2, 473-480, 2014.

(要約): Dexmedetomidine, a highly selective agonist of α2-adrenoceptors, is a commonly used sedative; however, a potent anti-inflammatory effect has also been found. In the present study we evaluated the inhibitory effect of locally injected dexmedetomidine on inflammatory responses in the injected region. Local inflammation was induced in the hindpaws of male mice (aged 6-8 weeks) by intraplantar injection of lambda-carrageenin. To offset the central effect of tested agents, different agents were blindly injected into the left and right paws in the pairs of comparison. The effect of dexmedetomidine on edema (increase in paw volume), the accumulation of leukocytes, and production of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were evaluated after carrageenin injection, using water displacement plethysmometry, histological imaging, immunohistochemistry, and Western blotting analysis. Furthermore, we also evaluated the effect of yohimbine, a full antagonist of α2-adrenoceptors, and phenylephrine, an agonist of the α1-adrenoceptor, on dexmedetomidine's action on inflammatory responses. Paw volume and amount of leukocytes in the injected region significantly increased after the injection of carrageenin. Similarly, TNF-α and COX-2 production was found in the subcutaneous region injected with carrageenin, 4 hours after injection. Dexmedetomidine significantly inhibited all increases in paw volume, leukocytes, and production of TNF-α and COX-2. Furthermore, yohimbine significantly antagonized the anti-inflammatory effects of dexmedetomidine, whereas phenylephrine did not significantly alter them. The findings suggest that locally injected dexmedetomidine exhibits an anti-inflammatory effect against local acute inflammatory responses, mediated by α2-adrenoceptors.
(キーワード): Adrenergic alpha-2 Receptor Antagonists / Anesthetics / Animals / Anti-Inflammatory Agents / Carrageenan / Cyclooxygenase 2 / Dexmedetomidine / Edema / Immunohistochemistry / Inflammation / Leukocytes / Male / Mice / Receptors, Adrenergic, alpha-2 / Time Factors / Tumor Necrosis Factor-alpha / Yohimbine
(出版サイトへのリンク): ● Publication site (DOI): 10.1213/ANE.0000000000000060
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24445644; ● Summary page in Scopus @ Elsevier: 2-s2.0-84893261202

(DOI: 10.1213/ANE.0000000000000060, PubMed: 24445644, Elsevier: Scopus) P. Andrea Rodriguez, Miho Inoue, Toshiyuki Tanaka, Michihiro Miyake, M. Ana Sfer, Etsuo Kishimoto, Hidetsugu Tsujigiwa, S. Rosario Rivera and Hitoshi Nagatsuka :
Effect of CaTiO₃-CaCO₃ prepared by alkoxide method on cell response,
Journal of Biomedical Materials Research. Part A, Vol.93, No.1, 297-303, 2010.

(要約): In recent years, calcium titanate (CaTiO(3)) and carbon-containing materials have gained much attention in a number of biomedical material researches. To maximize the advantages of both materials, we developed a novel alkoxide method to get "calcium titanate with calcium carbonate" (CaTiO(3)-CaCO(3)). The objective was to evaluate the crystallinity and elemental composition of CaTiO(3)-CaCO(3) prepared by alkoxide method, CaTiO(3)-aC elaborated by modified thermal decomposition method, commercially-prepared CaTiO(3), and the effect of these materials on the bone marrow stromal cell. Hydroxyapatite was used as positive control material. We examined the cellular proliferation, osteoblastic differentiation, and mineralization of KUSA/A1 cells cultured with the materials. The results showed that CaTiO(3)-CaCO(3) and CaTiO(3)-aC contained evidence of calcium carbonate enhancing cell proliferation, osteoblastic differentiation, and mineralization. On the contrary, the commercially-prepared CaTiO(3) revealed absence of calcium carbonate with lower cell response than the other groups. The results indicated that calcium carbonate could play a key role in the cell response of CaTiO(3) material. In conclusion, our findings suggest that CaTiO(3)-CaCO(3) could be considered an important candidate as a biomaterial for medical and dental applications.
(キーワード): Calcium titanate / Carbonate / Cell culture / Osteoblast / Particles
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.32551
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19562752; ● Summary page in Scopus @ Elsevier: 2-s2.0-77649225180

(DOI: 10.1002/jbm.a.32551, PubMed: 19562752, Elsevier: Scopus) Miho Inoue, P. Andrea Rodriguez, Noriyuki Nagai, Hitoshi Nagatsuka, Z. Racquel Legeros, Hidetsugu Tsujigiwa, Masahisa Inoue, Etsuo Kishimoto and Shin Takagi :
Effect of fluoride-substituted apatite on in vivo bone formation,
Journal of Biomaterials Applications, Vol.25, No.8, 811-824, 2010.

(要約): Biological apatites are characterized by the presence of minor constituents such as magnesium (Mg), chloride (Cl), or fluoride (F) ions. These ions affect cell proliferation and osteoblastic differentiation during bone tissue formation. F-substituted apatites are being explored as potential bonegraft materials. The aim of the present study is to investigate the mechanism of bone formation induced by fluoride-substituted apatite (FAp) by analyzing the effect of FAp on the process of in vivo bone formation. FAps containing different F concentrations (l-FAp: 0.48 wt%, m-FAp: 0.91 wt%, h-FAp: 2.23 wt%) and calcium-deficient apatite (CDA), as positive control, were implanted in rat tibia and bone formation was evaluated by histological examination, immuhistochemistry, in situ hybridization and tartrate-resistant acid phosphatase examinations. The results showed that l-FAp, m-FAp, h-FAp, and CDA biomaterials allowed migration of macrophages, attachment, proliferation, and phenotypic expression of bone cells leading to new bone formation in direct apposition to the particles. However, the l-FAp preparation allowed faster bone conduction compared to the other experimental materials. These results suggest that FAp with low F concentration may be an efficient bonegraft material for dental and medical application.
(キーワード): bone formation. / fluoride-substituted apatite
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/0885328209357109
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20219846; ● Summary page in Scopus @ Elsevier: 2-s2.0-79957646362

(DOI: 10.1177/0885328209357109, PubMed: 20219846, Elsevier: Scopus) Miho Inoue, AP Rodriguez, T Takagi, N Katase, M Kubota, N Nagai, H Nagatsuka, M Inoue, N Nagaoka, S Takagi and K Suzuki :
Effect of a new titanium coating material (CaTiO3-aC) prepared by thermal decomposition method on osteoblastic cell response.,
Journal of Biomaterials Applications, Vol.24, No.7, 657-672, 2010.

(要約): Titanium and hydroxyapatite (HA) are widely used as biomaterials for dental and medical applications. HA-coated titanium implants have excellent biocompatibility and mechanical properties. However, the adherence of HA film formed on titanium substrate is weak because of the lack of chemical interaction between HA and titanium. A solution to this problem is to form an intermediate film on titanium substrate, which provide excellent adherence to both titanium substrate and HA. We developed a novel biomaterial called calcium titanate-amorphous carbon (CaTiO(3)-aC) coating prepared by modified thermal decomposition method. The purpose of this study was to evaluate the effect of CaTiO(3)-aC and HA coating (positive control), and Ti (negative control) on osteoblastic (MT3T3-E1) cell responses. An increased cellular proliferation was observed in CaTiO(3)-aC coating compared to HA coating. The maximum expressions of ALP activity, Col I and ALP mRNA were higher and achieved in shorter period of time in CaTiO(3)-aC coating compared to others. These results demonstrated that CaTiO(3)-aC promoted better cell attachment, cellular proliferation, and osteoblastic differentiation compared with HA. In conclusion, we suggested that CaTiO(3)-aC could be considered as an important candidate as a coating material.
(キーワード): 3T3 Cells / Animals / Biocompatible Materials / Bone Substitutes / Cell Differentiation / Cell Proliferation / Cells, Cultured / Coated Materials, Biocompatible / Hot Temperature / Mice / Microscopy, Electron, Scanning / Microscopy, Electron, Transmission / Osteoblasts / Powders / Titanium
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/0885328209340334
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19726530; ● Search Scopus @ Elsevier (PMID): 19726530; ● Search Scopus @ Elsevier (DOI): 10.1177/0885328209340334

(DOI: 10.1177/0885328209340334, PubMed: 19726530) AP Rodriguez, H Tsujigiwa, M Gunduz, B Cengiz, N Nagai, R Tamamura, SS Borkosky, T Takagi, Miho Inoue and H Nagatsuka :
Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells.,
Biocell, Vol.33, No.1, 39-47, 2009.

(要約): Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
(キーワード): Animals / Bone Regeneration / Bone and Bones / Cell Differentiation / Cell Line / Collagen Type I / Dental Papilla / Diffusion Chambers, Culture / Gene Expression / Male / Mice / Mice, SCID / Odontoblasts / Odontogenesis / Osteoblasts / Osteocalcin / Osteonectin / Osteopontin / Protein Biosynthesis / Sialoglycoproteins
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19499885; ● Search Scopus @ Elsevier (PMID): 19499885

(PubMed: 19499885) GS Sathi, H Nagatsuka, R Tamamura, M Fujii, M Gunduz, Miho Inoue, RS Rivera and N Nagai :
Stromal cells promote bone invasion by suppressing bone formation in ameloblastoma.,
Histopathology, Vol.53, No.4, 458-467, 2008.

(要約): To study the stromal variation and role of stromal-tumour cell interaction in impaired bone formation as well as enhanced bone resorption in ameloblastoma. Four types of stroma were observed histologically; fibrous, desmoplastic, myxoid and myxoid with hyalinization. Osteoblast and osteoclast were counted using haematoxylin and eosin sections and immunohistochemistry with CD68. After histomorphometric analysis, only fibrous and myxoid types of stroma were distinctly identified. Secreted frizzled-related peptide (sFRP)-2, transforming growth factor-beta 1 and receptor activator of nuclear factor-kappaB ligand (RANKL) revealed strong expression in myxoid type compared with the normal stroma. Bone morphogenetic protein (BMP)-2 was negative in myxoid type, but positive in normal stroma. Fibrous-type stroma showed weak expression of all antigens except RANKL compared with myxoid type. The results suggest that stroma does not act only in bone resorption, but also in the suppression of new bone formation. sFRP-2 is the main factor for impaired bone formation. The expression of markers related to osteoclastogenesis and suppression of osteoblast formation is higher in myxoid-type than in fibrous-type stroma. Tumour cells create a favourable environment for impaired bone formation by secreting sFRP-2 as well as bone resorption by secreting RANKL and interleukin-6.
(キーワード): Adult / Ameloblastoma / Antigens, CD / Antigens, Differentiation, Myelomonocytic / Bone Neoplasms / Bone Resorption / Bone and Bones / Female / Humans / Interleukin-6 / Jaw Neoplasms / Male / Membrane Proteins / Middle Aged / Neoplasm Invasiveness / Osteoblasts / Osteoclasts / Osteogenesis / RANK Ligand / Stromal Cells / Tumor Cells, Cultured
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2559.2008.03127.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18983611; ● Search Scopus @ Elsevier (PMID): 18983611; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2559.2008.03127.x

(DOI: 10.1111/j.1365-2559.2008.03127.x, PubMed: 18983611) C Kuroda, S Kubota, K Kawata, E Aoyama, K Sumiyoshi, M Oka, Miho Inoue, S Minagi and M Takigawa :
Distribution, gene expression, and functional role of EphA4 during ossification.,
Biochemical and Biophysical Research Communications, Vol.374, No.1, 22-27, 2008.

(要約): EphA4 receptor tyrosine kinase has been shown to be critically involved in neural tissue development. Here, we found EphA4 was also distributed among hypertrophic chondrocytes and osteoblasts in the growth plate of developing mouse long bones. In vitro evaluation revealed that ephA4 expression was elevated upon hypertrophic differentiation of chondrocytes and that markedly stronger expression was observed in osteoblastic SaOS-2 than chondrocytic HCS-2/8 cells. Of note, RNAi-mediated silencing of ephA4 in SaOS-2 cells resulted in the repression of osteocalcin gene expression and alkaline phosphatase activity. Interestingly, confocal laser-scanning microscopic analysis revealed the presence of EphA4 molecules in the nucleus as well as on the surface of SaOS-2 cells. These findings are the first indication of a critical role of EphA4 in ossification, especially at the final stage in which osteoblasts and hypertrophic chondrocytes play major roles.
(キーワード): Alkaline Phosphatase / Animals / Cell Line / Cell Membrane / Cell Nucleus / Cells, Cultured / Chondrocytes / Gene Expression / Growth Plate / Humans / Mice / Osteoblasts / Osteocalcin / Osteogenesis / RNA Interference / RNA, Small Interfering / Receptor, EphA4
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbrc.2008.06.089
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18601903; ● Search Scopus @ Elsevier (PMID): 18601903; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbrc.2008.06.089

(DOI: 10.1016/j.bbrc.2008.06.089, PubMed: 18601903) M Okauchi-Yabuuchi, R Tamamura, N Nagaoka, S Takagi, E Kishimoto, T Takagi, AP Rodriguez, Miho Inoue, H Nagatsuka, M Akao and N Nagai :
Chemical Analysis of a Novel Coating Material, CaTiO3-aC.,
Journal of Hard Tissue Biology, Vol.17, No.3, 115-120, 2008.

(要約): Abstract:In the current study, we compared the recently developed CaTiO₃-amorphous carbon (CaTiO₃-aC) as a bone inducing coating material with HA from aspects of surface electric charge and solubility. CaTiO₃-aC had negative surface electric charge similar to HA. Thus similar tissue reaction and bone inducing ability were considered to obtain. On the other hand solubility of CaTiO₃-aC coating was lower than HA. Moreover though CaTiO₃-aC itself showed low solubility, CaCO₃ was found to be included in it, and long-term slow Ca²⁺ release occurred. Thus the sample was suggested to be used as an ion exchange material. When the implant using Ti/CaTiO₃-aC/HA double layer coating was developed, first bioactive state of the implant will continue due to HA character. Then even early resorption of HA occurs, bioactive state will continue due to CaTiO₃-aC layer. Therefore Ti base will not expose. This result is supposed to contribute long term success of the implant.
(キーワード): CaTiO₃-aC / Hydroxyapatite / Implant / Coating material / Titanium
(出版サイトへのリンク): ● Publication site (DOI): 10.2485/jhtb.17.115
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679435482624; ● Search Scopus @ Elsevier (DOI): 10.2485/jhtb.17.115

(DOI: 10.2485/jhtb.17.115, CiNii: 1390282679435482624) N Nagai, M Okauchi, AP Rodriguez, M Gunduz, H Hu, M Kubota, N Nagaoka, Miho Inoue, H Nagatsuka, T Takagi and M Akao :
Development of New Titanium Coating Material (CaTiO3-aC) with Modified Thermal Decomposition Method.,
Journal of Hard Tissue Biology, Vol.17, No.2, 47-54, 2008.

(要約): We developed a novel method for titanium coating material using modified thermal decomposition technique, specifically focusing on presence of CaTiO₃ and carbon C. Two layers of thin film coating composed of CaTiO₃-amorphous carbon compound (CaTiO_3-aC) and hydroxyapatite (HA) were generated on titanium surface. In this method, ratios of Ca/P as well as Ca/Ti, sintering temperature and sintering velocity were carefully planned. Within crystal structure of CaTiO₃ granules, 4 atomic % of carbon in amorphous state was included. By our method, inclusion of carbon in HA suggested formation of carbonate apatite. Regarding with attachment-detachment experiment at titanium surface, adhesion strength was 2.5 times stronger in CaTiO_3-aC/HA coating substrate as compared to HA only coating material. Results of the novel developed modified thermal decomposition method suggested that the 2 layers biomaterial composed of CaTiO_3-aC (0.6 μm) and carbonate apatite-included HA (2.4 μm) can be used as a coating material on titanium surface.
(キーワード): Biomaterial / Titanium / Carbon / HA / Coating material / CaTiO_3-aC
(出版サイトへのリンク): ● Publication site (DOI): 10.2485/jhtb.17.47
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390282679434524928; ● Search Scopus @ Elsevier (DOI): 10.2485/jhtb.17.47

(DOI: 10.2485/jhtb.17.47, CiNii: 1390282679434524928) M Hoshijima, T Hattori, Miho Inoue, D Araki, H Hanagata, A Miyauchi and M Takigawa :
CT domain of CCN2/CTGF directly interacts with fibronectin and enhances cell adhesion of chondrocytes through integrin alpha5beta1.,
FEBS Letters, Vol.580, No.5, 1376-1382, 2006.

(要約): Searching for CCN family protein 2/connective tissue growth factor (CCN2/CTGF) interactive proteins by yeast-two-hybrid screening, we identified fibronectin 1 gene product as a major binding partner of CCN2/CTGF in the chondrosarcoma-derived chondrocytic cell line HCS-2/8. Only the CT domain of CCN2/CTGF bound directly to fibronectin (FN). CCN2/CTGF and its CT domain enhanced the adhesion of HCS-2/8 cells to FN in a dose-dependent manner. The CCN2/CTGF-enhancing effect on cell adhesion to FN was abolished by a blocking antibody against alpha5beta1 integrin (alpha5beta1), but not by one against anti-alphavbeta3 integrin. These findings suggest for the first time that CCN2/CTGF enhances chondrocyte adhesion to FN through direct interaction of its C-terminal CT domain with FN, and that alpha5beta1 is involved in this adhesion.
(キーワード): Binding Sites / Cell Adhesion / Cell Line / Chondrocytes / Connective Tissue Growth Factor / Fibronectins / Humans / Immediate-Early Proteins / Integrin alpha5beta1 / Intercellular Signaling Peptides and Proteins / Membrane Proteins / Protein Binding / Two-Hybrid System Techniques
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.febslet.2006.01.061
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16457822; ● Search Scopus @ Elsevier (PMID): 16457822; ● Search Scopus @ Elsevier (DOI): 10.1016/j.febslet.2006.01.061

(DOI: 10.1016/j.febslet.2006.01.061, PubMed: 16457822) RG Craig, AR Kamer, SP Kallur, Miho Inoue and DP Tarnow :
Effects of periodontal cell grafts and enamel matrix proteins on the implant-connective tissue interface: a pilot study in the minipig.,
The Journal of Oral Implantology, Vol.32, No.5, 228-236, 2006.

(要約): We have developed an experimental model to help identify and characterize factors necessary for periodontal connective tissue attachment formation on dental implants. In this pilot study, we report the effect of autogenous periodontal cell grafts, with and without the a pplication of enamel matrix derivative (EMD), on the implant-connective tissue interface. Periodontal ligament (PDL) and gingival connective tissue (GCT) cultures were established from an adult minipig. Implants were placed in osteotomies prepared with exaggerated countersinks that served as recipient sites for autogenous cell grafts in bilateral edentulated posterior mandibular sextants. In addition, 1 side received an application of EMD before placement of the autogenous cell grafts. A bioabsorbable membrane covering the coronal portion of the implants was placed before closure. After 8 weeks, quantitative histomorphometric and qualitative light microscopic analyses revealed that the implants that received gelatin vehicle alone were surrounded by bone, whereas the implants that received GCT cell grafts were mostly surrounded by fibrous connective tissue. In contrast, implants that received PDL cells without the application of EMD demonstrated good bone contact, but strands of epithelium were observed in the implant-connective tissue interface. Implants that received PDL cells and EMD also had good bone contact but without evidence of epithelium. A cementum-like interface was not observed in any of the groups. Results of this pilot study suggest that EMD and the type of cell populations present in the implant wound-healing environment may alter the implant-connective tissue interface.
(キーワード): Animals / Connective Tissue Cells / Dental Enamel Proteins / Dental Implantation, Endosseous / Dental Implants / Female / Gingiva / Models, Animal / Osseointegration / Periodontal Ligament / Pilot Projects / Regeneration / Swine / Swine, Miniature
(出版サイトへのリンク): ● Publication site (DOI): 10.1563/820.1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17069167; ● Search Scopus @ Elsevier (PMID): 17069167; ● Search Scopus @ Elsevier (DOI): 10.1563/820.1

(DOI: 10.1563/820.1, PubMed: 17069167) H Tsujigiwa, H Nagatsuka, M Gunduz, AP Rodriguez, RS Rivera, RZ LeGeros, Miho Inoue and N Nagai :
Effects of immobilized recombinant human bone morphogenetic protein-2/succinylated type I atelocollagen on cellular activity of ST2 cells.,
Journal of Biomedical Materials Research. Part A, Vol.75A, No.1, 210-215, 2005.

(要約): The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) to induce ectopic bone formation requires a carrier. Type I atelocollagen, a biomaterial with a porous structure, excellent operational features, and biocompatibility, is an effective carrier for rhBMP-2. However, the conventionally used lyophilized rhBMP-2/collagen mixture does not necessarily give adequate bone-induction effect. In the present study, we examined the effect of immobilizing rhBMP-2 to type I atelocollagen on the cellular activity of ST2 cells. The following results were obtained: (1) rhBMP-2 was effectively immobilized to succinylated type I atelocollagen, indicating the usefulness of succinylated type I atelocollagen in immobilization; (2) studies of alkaline phosphatase activity confirmed the effectiveness of rhBMP-2 immobilized on succinylated atelocollagen in augmenting cellular activity.
(キーワード): Alkaline Phosphatase / Animals / Biocompatible Materials / Blotting, Western / Bone Marrow Cells / Bone Morphogenetic Protein 2 / Bone Morphogenetic Proteins / Bone and Bones / Cell Line / Collagen / Collagen Type I / Diffusion / Dose-Response Relationship, Drug / Electrophoresis, Polyacrylamide Gel / Humans / Mice / Recombinant Proteins / Succinates / Time Factors / Transforming Growth Factor beta
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.30416
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16059889; ● Search Scopus @ Elsevier (PMID): 16059889; ● Search Scopus @ Elsevier (DOI): 10.1002/jbm.a.30416

(DOI: 10.1002/jbm.a.30416, PubMed: 16059889) Miho Inoue, H Nagatsuka, H Tsujigiwa, M Inoue, RZ LeGeros, T Yamamoto and N Nagai :
In vivo effect of fluoride-substituted apatite on rat bone.,
Dental Materials Journal, Vol.24, No.3, 398-402, 2005.

(要約): Different types of calcium phosphate compounds are commercially available for medical and dental applications as bone substitute materials. Biological apatites contain several kinds of minor elements such as carbonate (CO3), magnesium (Mg), and fluoride (F) in enamel, dentin, and bone. It has been shown that F ion and F-substituted apatite promoted osteoblast proliferation and inhibited osteoclast cell activity. The purpose of this study was to investigate the in vivo rat tibia activity on F-substituted apatite (FAp). Apatites of unsintered calcium deficient apatite (CDA), and FAps, with low, medium, and high F concentrations, were implanted in rat tibia for 1 and 2 weeks. Implanted tissues were embedded in paraffin blocks, stained with hematoxylin-eosin and histomorphometrically observed. Results showed that low F concentration induced better and faster new bone formation in vivo compared to CDA. Therefore the results suggested that F as a minor element in bone rendered a suitable effect on bone formation in vivo.
(キーワード): Animals / Apatites / Bone Marrow / Bone Substitutes / Calcium / Coloring Agents / Fluorides / Male / Osteoblasts / Osteoclasts / Osteogenesis / Rats / Rats, Wistar / Tibia / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.4012/dmj.24.398
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16279730; ● Search Scopus @ Elsevier (PMID): 16279730; ● Search Scopus @ Elsevier (DOI): 10.4012/dmj.24.398

(DOI: 10.4012/dmj.24.398, PubMed: 16279730) H Nakamura, N Nagaoka, A Hirata, Miho Inoue, H Ozawa and T Yamamoto :
Distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) in osteoclasts after calcitonin administration.,
Archives of Histology and Cytology, Vol.68, No.2, 143-150, 2005.

(要約): Scanning electron microscopy (SEM) was employed to study the effect of calcitonin on the distribution of actin filaments in osteoclasts obtained from rat tibiae. Fluorescent microscopy was also applied to examine calcitonin-induced changes in the distribution of actin filaments, non-muscle myosin, M-Ras, and extracellular signal-regulated kinase (ERK) to clarify the role of ERK in the cytoskeleton of osteoclasts. SEM of control osteoclasts revealed a ring-like structure in the peripheral region. Labeled actin filaments and non-muscle myosin were detected in the peripheral region and exhibited a ring-like pattern. Immunoreactivity indicating M-Ras and ERK was also detected in the vicinity of the actin ring. After calcitonin treatment, many osteoclasts exhibited a retracted appearance and lacked a discernible actin ring. Numerous retraction fibers were found at the edge of calcitonin-treated osteoclasts. Actin filaments and non-muscle myosin were concentrated in the cytoplasm of calcitonin-treated osteoclasts, and exhibited a filamentous pattern. Labeled M-Ras and ERK also accumulated in the central region of these osteoclasts. These findings suggest that actin-myosin interaction plays an essential role in the retraction of osteoclasts induced by calcitonin. ERK may play a role in this interaction by activating myosin light chain kinase, as previously observed in smooth muscle cells.
(キーワード): Actins / Animals / Calcitonin / Cytoskeletal Proteins / Extracellular Signal-Regulated MAP Kinases / Immunohistochemistry / Microscopy, Electron, Scanning / Monomeric GTP-Binding Proteins / Myosins / Osteoclasts / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1679/aohc.68.143
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16079460; ● Search Scopus @ Elsevier (PMID): 16079460; ● Search Scopus @ Elsevier (DOI): 10.1679/aohc.68.143

(DOI: 10.1679/aohc.68.143, PubMed: 16079460) T Yamamoto, N Nagaoka, A Hirata, H Nakamura, Miho Inoue, M Kawai and M Ikegame :
Ultrastructural and imminohistochemical studies of medullary bone calcification, with special reference to sulphated glycosaminoglycans.,
Journal of Electron Microscopy, Vol.54, No.1, 29-34, 2005.

(要約): Histochemical, immunohistochemical and electron energy-loss spectroscopic studies were performed to examine the relationship between sulphated glycosaminoglycans and medullary bone calcification using oestrogen-injected male Japanese quail. Sulphated glycosaminoglycans, detected by high iron diamine (HID) or HID-thiocarbohydrazide-silver protein (HID-TCH-SP) methods, were distributed throughout the matrix of medullary bone, some periphery and extending tips of the trabeculae stained weakly, and the globular structures at osteoid areas were exclusively positive for HID-TCH-SP stain. Immunohistochemistry identified keratan sulphate located in the globular structures at osteoid areas and calcified matrix, but chondroitin-4 sulphate and chondroitin-6 sulphate were not detected in the matrix. Using electron spectroscopic imaging, sulphur was determined to be localized in the globular structures. These results demonstrate that medullary bone matrix accumulates keratan sulphate in the globular structures, which are the foci for calcification, and eventually in the calcified areas. This suggests that keratan sulphate containing sulphur is maintained in the calcified matrix. These results indicate a unique process of calcification exists in medullary bone.
(キーワード): Animals / Bone Matrix / Bone and Bones / Calcification, Physiologic / Chondroitin Sulfates / Coturnix / Estrogens / Glycosaminoglycans / Histocytochemistry / Hydrazines / Immunohistochemistry / Keratan Sulfate / Male / Silver Proteins / Spectroscopy, Electron Energy-Loss
(出版サイトへのリンク): ● Publication site (DOI): 10.1093/jmicro/dfh097
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15695482; ● Search Scopus @ Elsevier (PMID): 15695482; ● Search Scopus @ Elsevier (DOI): 10.1093/jmicro/dfh097

(DOI: 10.1093/jmicro/dfh097, PubMed: 15695482) H Nakamura, R Kato, A Hirata, Miho Inoue and T Yamamoto :
Localization of CD44 (Hyaluronan Receptor) and Hyaluronan in Rat Mandibular Condyle.,
The Journal of Histochemistry and Cytochemistry, Vol.53, No.1, 113-120, 2005.

(要約): CD44 is a multifunctional adhesion molecule that binds to hyaluronan (HA), type I collagen, and fibronectin. We investigated localization of CD44 and HA in mandibular condylar cartilage compared with the growth plate and the articular cartilage, to clarify the characteristics of chondrocytes. We also performed Western blotting using a lysate of mandibular condyle. In mandibular condyle, CD44-positive cells were seen in the surface region of the fibrous cell layer and in the proliferative cell layer. Western blotting revealed that the molecular weight of CD44 in condyle was 78 to 86 kD. Intense reactivity for HA was detected on the surface of the condyle and the lacunae of the hypertrophic cell layer. Moderate labeling was seen in cartilage matrix of the proliferative and maturative layer. Weak labeling was also seen in the fibrous cell layer. In growth plate and articular cartilage, HA was detected in all cell layers. However, chondrocytes of these cartilages did not exhibit reactivity for CD44. These results suggest that chondrocytes in the mandibular condylar cartilage differ in expression of CD44 from those in tibial growth plate and articular cartilage. Cell-matrix interaction between CD44 and HA may play an important role in the proliferation of chondrocytes in the mandibular condyle.
(キーワード): Animals / Cartilage, Articular / Cell Differentiation / Chondrocytes / Electrophoresis, Polyacrylamide Gel / Growth Plate / Hyaluronan Receptors / Hyaluronic Acid / Mandibular Condyle / Microscopy, Immunoelectron / Molecular Weight / Rats / Tibia
(出版サイトへのリンク): ● Publication site (DOI): 10.1177/002215540505300113
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15637344; ● Search Scopus @ Elsevier (PMID): 15637344; ● Search Scopus @ Elsevier (DOI): 10.1177/002215540505300113

(DOI: 10.1177/002215540505300113, PubMed: 15637344) Miho Inoue, RZ LeGeros, M Inoue, R Rohanizadeh, L Guo, R Davidson, K Rui, H Nagatsuka and N Nagai :
Effect of magnesium, strontium or fluoride ions on in vitro activities of odontoblast-like cells (MDPC-23).,
Journal of Hard Tissue Biology, Vol.14, No.1, 5-12, 2005.

(出版サイトへのリンク): ● Publication site (DOI): 10.2485/jhtb.14.5
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.2485/jhtb.14.5

(DOI: 10.2485/jhtb.14.5) Miho Inoue, RZ LeGeros, M Inoue, H Tsujigiwa, H Nagatsuka, T Yamamoto and N Nagai :
In vitro response of osteoblast-like and odontoblast-like cells to unsubstituted and substituted apatites.,
Journal of Biomedical Materials Research. Part A, Vol.70A, No.4, 585-593, 2004.

(要約): Different types of calcium phosphate compounds [calcium-deficient apatite (CDA); beta-tricalcium phosphate (beta-TCP); biphasic calcium phosphate (BCP)] are commercially available for medical and dental applications as bone substitute materials. Most of the reported in vitro studies on cell-material interactions have used osteoblast-like cells. The purpose of this study was to investigate the in vitro response of osteoblast-like (MC3T3-E1) and odontoblast-like (MDPC23) cells on unsubstituted (HA) and substituted (F-substituted) apatites. MC3T3-E1 and MDPC23 were cultured in alpha-modified medium containing 10% fetal bovine serum, ascorbic acid (50 microg/mL) and beta-glycerophosphate (2 mM). The cells were seeded on pellets made from HA, and FAp (with low, medium, and high F concentrations). Cell morphology was observed after 7 and 14 days using scanning electron microscopy (SEM). Cell attachment and differentiation were determined from the DNA content, alkaline phosphatase (ALP) activity, and total collagen content. Pellet surface composition was characterized by using Fourier Transform infrared spectroscopy. MC3T3-E1 and MDPC23 cells on HA were normal in shape and in fusion but not on FAp. Results of this study showed that the pattern of cell proliferation of osteoblast-like cells was different from that of the odontoblast-like cells. This study suggests that cell morphology, fusion, and proliferation on biomaterial surfaces depend on cell type (osteoblast-like vs odontoblast-like cell) and biomaterial composition (unsubstituted vs substituted F-apatites).
(キーワード): Alkaline Phosphatase / Animals / Apatites / Cell Line / Collagen / DNA / Mice / Microscopy, Electron, Scanning / Odontoblasts / Organ Specificity / Osteoblasts / Spectroscopy, Fourier Transform Infrared
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.30116
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15307163; ● Search Scopus @ Elsevier (PMID): 15307163; ● Search Scopus @ Elsevier (DOI): 10.1002/jbm.a.30116

(DOI: 10.1002/jbm.a.30116, PubMed: 15307163) RG Craig, SP Kallur, Miho Inoue, PA Rosenberg and RZ LeGeros :
Effect of enamel matrix proteins on the periodontal connective tissue-material interface after wound healing.,
Journal of Biomedical Materials Research. Part A, Vol.69A, No.1, 180-187, 2004.

(要約): The periodontal ligament has the potential to regenerate a complete periodontal connective tissue attachment, starting with the deposition of cementum, on pathologically exposed root surfaces as well as several materials including titanium oxide. However, most commonly used dental materials result in a fibrous encapsulation or a chronic inflammatory response after periodontal wound healing rather than the formation of a periodontal connective tissue attachment. Recently, an extract of porcine enamel matrix (Emdogain(R), EMD) has been reported inductive of cementum formation in both in vivo and in vitro studies. The aim of this study was to determine the effect of EMD, when applied to materials previously reported not supportive of periodontal connective tissue formation, on the periodontal connective tissue-material interface obtained with these materials in vivo. Bilateral osteotomies were performed on the mandible of a Yucatan minipig exposing the buccal root surface of four premolars. A series of four preparations were placed in each root surface that were subsequently filled with calcium hydroxide, gutta percha, mineral trioxide aggregate (MTA), or left unfilled. One side, in addition, received an application of EMD prior to surgical closure. A bioabsorbable surgical barrier membrane was placed over the osteotomy sites to exclude gingival connective tissue from the wound-healing environment. The mucoperiosteal flaps were then readapted and sutured in position. The animal was euthanized 10 weeks after the procedure, block sections obtained and prepared for light microscopy. Results demonstrated complete regeneration of alveolar bone and periodontal ligament in all four teeth from the EMD-treated side. Fibers from the periodontal ligament were observed to insert into a mineralized matrix consistent with cementum on all four root preparations. In contrast, massive root resorption without regeneration of alveolar bone was found on all teeth from the side not treated with EMD. The results of this pilot study suggest that the application of EMD to material surfaces that normally do not support periodontal connective tissue attachment formation can alter the type of periodontal connective tissue interface obtained with these materials.
(キーワード): Animals / Bone Cements / Calcium Hydroxide / Cementogenesis / Dental Enamel Proteins / Dental Materials / Female / Guided Tissue Regeneration / Membranes, Artificial / Periodontal Ligament / Swine / Swine, Miniature / Tooth Root / Wound Healing
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.20140
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14999766; ● Search Scopus @ Elsevier (PMID): 14999766; ● Search Scopus @ Elsevier (DOI): 10.1002/jbm.a.20140

(DOI: 10.1002/jbm.a.20140, PubMed: 14999766) Miho Inoue, RZ LeGeros, C Hoffman, K Diamond, PA Rosenberg and RG Craig :
Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials.,
Journal of Biomedical Materials Research. Part A, Vol.69A, No.1, 172-179, 2004.

(要約): Cells within the periodontal ligament have the potential to regenerate a periodontal connective tissue attachment on pathologically exposed root surfaces as well as on several material surfaces including titanium. However, rather than a periodontal connective tissue attachment, a fibrous encapsulation or chronic inflammatory response has been reported at the material connective tissue interface for most dental materials. Cementum is the first tissue of the periodontal connective tissue attachment to develop and the secretion of enamel matrix related proteins on the newly mineralized dentin surface precedes and is thought to induce cementum formation. Enamel matrix-related proteins may also function in the adult because the application of an acid extract of porcine enamel protein matrix (Emdogain(R), EMD) on pathologically exposed root surfaces has been shown to result in cementum regeneration. Therefore, the objective of the present study was to determine whether the application of EMD to materials that do not normally support cementogenesis in vivo would alter the in vitro phenotype of periodontal ligament (PDL) cells including the synthesis of cementum-associated extracellular matrix proteins. Primary PDL cells were established from 21-day-old Sprague-Dawley rats, and were cultured on four materials commonly encountered in dental practice (gutta percha, calcium hydroxide, amalgam, and super EBA cement) with and without the application of EMD. After 7 or 14 days of culture, total-DNA content, collagen synthesis, alkaline phosphatase activity, and the synthesis of a 42-kDa cementum-associated extracellular matrix protein were determined. PDL cells cultured on all materials had decreased total DNA content. The application of EMD further decreased total DNA content. PDL cells cultured on gutta percha and calcium hydroxide with the application of EMD had similar levels of collagen synthesis and alkaline phosphatase activity but also expressed a 42-kDa cementum extracellular matrix-associated protein when compared to the other groups. These results suggest that EMD can alter the phenotype of PDL cells when cultured on these dental materials.
(キーワード): Alkaline Phosphatase / Animals / Bone Cements / Calcium Hydroxide / Cell Differentiation / Cells, Cultured / Collagen / DNA / Dental Enamel Proteins / Dental Materials / Extracellular Matrix Proteins / Gene Expression / Gingiva / Periodontal Ligament / Phenotype / Rats / Rats, Sprague-Dawley
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/jbm.a.20139
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 14999765; ● Search Scopus @ Elsevier (PMID): 14999765; ● Search Scopus @ Elsevier (DOI): 10.1002/jbm.a.20139

(DOI: 10.1002/jbm.a.20139, PubMed: 14999765) K Nawachi, Miho Inoue, S Kubota, K Takahashi, T Nishida, G Yoshimichi, T Nakanishi, M Kanyama, T Kuboki, H Yatani, T Yamaai and M Takigawa :
Tyrosine Kinase-type receptor ErbB4 in chondrocytes : interaction with connective tissue growth factor and distribution in cartilage.,
FEBS Letters, Vol.528, No.1-3, 109-113, 2002.

(要約): In order to identify receptor molecules that participate in the growth and differentiation of chondrocytes, we cloned a number of cDNA fragments from HCS-2/8 chondrocytic cells, by using tyrosine kinase-specific primers for amplification. The mRNA expression of one such receptor, ErbB4, was increased by connective tissue growth factor/hypertrophic chondrocyte-specific gene product (CTGF/Hcs24), which promotes all stages of the endochondral ossification in vitro. ErbB4 expression was observed through all stages of chondrocytic differentiation in vitro, corresponding to the wide distribution of CTGF/Hcs24 target cells. Furthermore, positive signals for erbB4 mRNA were detectable throughout most populations of chondrocytes, in growth and articular cartilage in vivo. These results demonstrate for the first time that ErbB4 is expressed in chondrocytes and may play some roles in chondrocytic growth and differentiation along with CTGF/Hcs24.
(キーワード): Amino Acid Sequence / Animals / Cartilage / Cartilage, Articular / Cell Differentiation / Cell Line / Chondrocytes / Connective Tissue Growth Factor / ErbB Receptors / Growth Plate / Humans / Immediate-Early Proteins / Intercellular Signaling Peptides and Proteins / Mice / Mice, Inbred ICR / Molecular Sequence Data / Protein Structure, Tertiary / Receptor, ErbB-4 / Recombinant Proteins / Sequence Homology, Amino Acid / Tissue Distribution / Up-Regulation
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/s0014-5793(02)03263-5
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12297288; ● Search Scopus @ Elsevier (PMID): 12297288; ● Search Scopus @ Elsevier (DOI): 10.1016/s0014-5793(02)03263-5

(DOI: 10.1016/s0014-5793(02)03263-5, PubMed: 12297288) Miho Inoue, T Inoue, A Miyagi, I Tanimoto, R Shingaki, h Ohta and K Fukui :
Nucleotide Sequencing and Transcriptional Analysis of Two Tandem Genes Encoding Glucosyltransferase (Water- Soluble- Glucan Synthetase) in Streptococcus cricetus HS-6.,
Microbiology and Immunology, Vol.44, No.9, 755-764, 2000.

(要約): Two tandem genes encoding glucosyltransferase synthesizing water-soluble glucan (GTF-S) were cloned from the lambda gene library of Streptococcus cricetus HS-6 (serotype a) using anti-GTF-S antibody, and the nucleotide sequences were analyzed. The two genes (ORF1 and ORF2) were identified as streptococcal glucosyltransferases based on the following evidence: [1] the deduced amino acid sequences of their products have an active site for catalytic action and C-terminal repeated units for dextran binding, and [2] a homology search revealed that the ORF1 and ORF2 products are homologous to the GtfS protein (77.4%) of S. downei Mfe28 and GtfT protein (83.8%) of S. sobrinus OMZ176, respectively, which are both known to have GTF-S activity. Therefore, ORF1 and ORF2 might be designated gtfS and gtfT of S. cricetus, respectively. A Northern blotting and RNase protection assay suggested that the gtfS and gtfT of S. cricetus are transcribed as a single bicistronic mRNA as well as separate monocistronic mRNAs. Primer extension analysis indicated multiple transcriptional start points for each gene.
(キーワード): Amino Acid Sequence / Base Sequence / Cloning, Molecular / Glucosyltransferases / Molecular Sequence Data / Open Reading Frames / Streptococcus / Transcription, Genetic
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1348-0421.2000.tb02560.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11092239; ● Search Scopus @ Elsevier (PMID): 11092239; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1348-0421.2000.tb02560.x

(DOI: 10.1111/j.1348-0421.2000.tb02560.x, PubMed: 11092239) S Kawamata, K Shimoharai, Y Imura, Miho Inoue, Y Ichinose, T Shiraishi, H Kunoh and T Yamada :
Temporal and Spatial Pattern of the Pea Phenylalanine Ammonia-Lyase Gene1 Promoter in Transgenic Tabacco.,
Plant & Cell Physiology, Vol.38, No.7, 792-803, 1997.

MISC

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総説・解説

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講演・発表

S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka :
CGRP induced glial-cytokine expression, NFkB signaling and orofacial nociception,
International Association for Dental Research, Washington, D.C., Mar. 2020. R Raju, Masamitsu Ohshima, Tsuyoshi Morita, Huijiao Yan, Miho Inoue, Otto Baba, Masahisa Inoue and Yoshizo Matsuka :
A double-layered complex cell sheet can regenerate the periodontal tissue in a mice model,
Dentisphere, Surabayai, Nov. 2019. S Afroz, Rieko Arakaki, Takuma Iwasa, Masamitsu Ohshima, Maki Hosoki, Miho Inoue, Otto Baba, Yoshihiro Okayama and Yoshizo Matsuka :
Orofacial nociception due to glial expressed cytokines in trigeminal ganglia,
International Association for Dental Research, Vancouver, Jun. 2019. Iwasa Takuma, Rieko Arakaki, Oshima Masamitsu, S Afroz, Miho Inoue, Goto Nami, Naozumi Ishimaru and Yoshizo Matsuka :
Up-regulation of inflammatory cytokines in trigeminal ganglia after infraorbital nerve constriction,
The Journal of Neuroscience, Washington DC, Nov. 2017. P. Andrea Rodríguez, A. María Sánchez, Miho Inoue, Betiana Felice, Hitoshi Nagatsuka, E. Rossana Madrid, Carmelo Felice and Hidetsugu Tsujigiwa :
Effect of sintering time of CaTiO₃-CaCO₃ in osteoblastic response of KUSA/A1 cells,
IFMBE Proceedings, Vol.49, 159-162, Jan. 2015.

(キーワード): Calcium titanate / Carbonate / Cell culture / Osteoblast / Particles
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/978-3-319-13117-7_42
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-84925277703

(DOI: 10.1007/978-3-319-13117-7_42, Elsevier: Scopus) 谷脇竜弥, 細木眞紀, 大島正充, 大倉一夫, 鈴木善貴, 井上美穂, 生田目大介, 宮城麻友, 板東伸幸, 小澤彩, 吉原靖智, 新開瑞希, 柴垣あかり, 青木映璃, 鴨居浩平, 松香芳三 :
徳島大学における補綴学実習の評価と改善,
大学教育カンファレンスin徳島, 2024年12月. 新開瑞希, 鈴木善貴, 大倉一夫, 小澤彩, 柴垣あかり, 谷脇竜弥, 井上美穂, 松香芳三 :
口腔内湿潤および唾液分泌量と睡眠時ブラキシズムの関連の調査,
日本睡眠歯科学会, 2024年11月. 谷脇竜弥, 大倉一夫, 鈴木善貴, 井上美穂, 新開瑞希, 松香芳三 :
糖質制限による睡眠時ブラキシズムへの効果の検討, 中間報告,
日本睡眠歯科学会, 2024年11月. 井上美穂, 鈴木善貴, 新開瑞希, 谷脇竜弥, 大倉一夫, 松香芳三 :
睡眠時ブラキシズムに対する各種精油の効果,
日本睡眠歯科学会, 2024年11月. 小池一幸, 細木眞紀, 田島登誉子, 宮城麻友, 井上美穂, 生田目大介, 吉原靖智, 谷脇竜弥, 大島正充, 松香芳三 :
掌蹠膿疱症性骨関節炎における歯科金属除去の重要性:症例報告と考察,
日本補綴学会中国四国支部学術大会, 2024年8月. 井上正久, 岡本果鈴, 川上隆茂, 阿部友美, 井上美穂, 細木眞紀, 松香芳三 :
チタン及びニッケル誘発アレルギーマウスにおける好酸球局在の免疫組織化学的検討,
硬組織再生生物学会, 2024年8月. 谷脇竜弥, 細木眞紀, 宮城麻友, 板東伸幸, 大島正充, 鈴木善貴, 井上美穂, 生田目大介, 小澤彩, 青木映璃, 吉原靖智, 大倉一夫, 新開瑞希, 柴垣あかり, 松香芳三 :
クラウンブリッジ補綴学模型実習の改善:アンケート調査に基づく分析,
日本補綴歯科学会雑誌, 2024年7月. 小澤彩, 鈴木善貴, 大倉一夫, 田島登誉子, 谷脇竜弥, 新開瑞希, 柴垣あかり, 井上美穂, 大川敏永, 安陪晋, 重本修伺, 松香芳三 :
睡眠時ブラキシズムはオクルーザルアプライアンスを変形させるのか,
日本補綴歯科学会雑誌, 2024年7月. 横井美有希, 安部倉仁, 森田晃司, 土井一矢, 細木眞紀, 宮城麻友, 井上美穂, 生田目大介, 中川晋輔, 秋山謙太郎, 縄稚久美子, 黒崎陽子, 松香芳三, 窪木拓男, 津賀一弘 :
最後方大臼歯へのPEEKクラウンの臨床応用についての6ヶ月間の評価,
日本補綴歯科学会雑誌, 2024年7月. 細木眞紀, 田島登誉子, 宮城麻衣, 井上美穂, 仁木真理子, 久保宜明, 松香芳三 :
金属アレルギー患者におけるピアスによる皮膚障害の臨床的検討,
日本アレルギー学会, Vol.72, No.6, 871, 2023年10月. 青木映璃, 鈴木善貴, 新開瑞希, 小澤彩, 柴垣あかり, 谷脇竜弥, 吉原靖智, 大倉一夫, 井上美穂, 池田隆志, 大島正充, 松香芳三 :
睡眠体位療法による睡眠時ブラキシズムへの効果の検討,
日本補綴学会中国四国支部学術大会, 2023年9月. 大島正充, 井上美穂, 宮城麻友, 松香芳三 :
複合組織の立体構築による歯の組織再生技術の開発,
公益社団法人日本補綴歯科学会令和5年度中国四国支部学術大会, 2023年9月. 柴垣あかり, 鈴木善貴, 福田杏, 神野青空, 新開瑞希, 小澤彩, 青木映璃, 谷脇竜弥, 大倉一夫, 井上美穂, G. Lavigne, 松香芳三 :
睡眠体位療法の睡眠時ブラキシズムへの効果予測の検討,
四国歯学会雑誌, 2023年7月. 小澤彩, 鈴木善貴, 田島登誉子, 大川敏永, 鴨居浩平, 大倉一夫, 谷脇竜弥, 井上美穂, 吉原靖智, 大島正充, 安陪晋, 松香芳三 :
睡眠時ブラキシズム患者の適応したアプライアンスの変形を長期間観察した症例,
日本補綴歯科学会雑誌, 2023年5月. 井上美穂, 大島正充, 宮城麻友, 生田目大介, 松香芳三 :
口腔顔面痛はアロマテラピーで軽減する∼口腔顔面痛モデル動物での評価∼,
日本補綴歯科学会雑誌, 2023年5月. 細木眞紀, 生田目大介, 宮城麻友, 大倉一夫, 井上美穂, 小澤彩, 柴垣あかり, 谷脇竜弥, 鴨居浩平, 板東伸幸, 吉原靖智, 大島正充, 鈴木善貴, 新開瑞希, 松香芳三 :
デジタル化に対応したクラウンブリッジ補綴学実習の検討,
日本補綴歯科学会雑誌, 2023年5月. 蓮池真由子, 井上美穂, Swarna Raman, Jaime M.Fabillar, Lutfi P.Perdana, 宮城麻友, 生田目大介, 大島正充, 松香芳三 :
口腔顔面痛モデル動物を用いた精油嗅覚刺激の疼痛抑制効果∼ベルガモット，ラベンダーの検討∼,
四国歯学会雑誌, 2023年3月. 井上美穂, Swarna Raman, Jaime Jr.M.Fabillar, 蓮池真由子, Lutfi P.Perdana, 井上正久, 生田目大介, 宮城麻友, 大島正充, 松香芳三 :
精油成分による口腔顔面痛モデル動物の痛み軽減とメカニズム,
徳島大学脳科学クラスター・ミニリトリート, 2023年2月. 細木眞紀, 生田目大介, 宮城麻友, 大倉一夫, 井上美穂, 小澤彩, 柴垣あかり, 谷脇竜弥, 鴨居浩平, 板東伸幸, C. Riquelme, 吉原靖智, 大島正充, 鈴木善貴, 新開みずき, 松香芳三 :
デジタル化が進む歯科診療の現状紹介と歯科補綴学実習における学習内容の検討,
大学教育カンファレンスin徳島, 2022年12月. 細木眞紀, 田島登誉子, 宮城麻友, 井上美穂, 久保宜明, 松香芳三 :
装飾品に対するニッケル表示の標準化をめざした取り組みについて,
日本皮膚免疫アレルギー学会, 2022年12月. 大倉一夫, 井上美穂, 鈴木善貴, 細木眞紀, 生田目大介, 安陪晋, 河野文昭, 松香芳三 :
アクティブラーニングの学修効果 ―オンライン授業と通常授業，TBL授業の比較―,
日本歯科医学教育学会雑誌, 2022年7月. 細木眞紀, 大倉一夫, 井上美穂, 鈴木善貴, 生田目大介, 安陪晋, 河野文昭, 松香芳三 :
2021年度歯科補綴学実習の評価,
日本歯科医学教育学会雑誌, 2022年7月. 田島登誉子, 細木眞紀, 井上美穂, 小澤彩, 新開瑞希, 宮城麻友, 成谷美緒, 大島正充, 杉尾隆夫, 山本伊一郎, 松香芳三 :
徳島大学病院における歯科用金属アレルギー患者の臨床統計学的検討,
日本補綴歯科学会雑誌, 2022年7月. 新開瑞希, 細木眞紀, 鈴木善貴, 田島登誉子, 井上美穂, 小澤彩, 吉原靖智, 大島正充, 織田英正, 本田常晴, 松香芳三 :
レジンアレルギーのリスクと歯科治療における対応,
日本補綴歯科学会雑誌, 2022年7月. 大倉一夫, 葉山莉香, 大島正充, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂, 生田目大介, 田島登誉子, 吉原靖智, 小澤彩, 新開瑞希, 松香芳三 :
歯科補綴学におけるアクティブラーニングの学修効果反転授業とTBL，通常授業の比較,
日本補綴歯科学会雑誌, 2022年7月. 大倉一夫, 葉山莉香, 大島正充, 細木眞紀, 鈴木善貴, 井上美穂, 生田目大介, 吉原靖智, 新開瑞希, 小澤彩, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの学修効果―オンライン授業と通常授業，TBL授業の比較―,
大学教育カンファレンス in 徳島, 2022年1月. 細木眞紀, 西川泰史, 生田目大介, 大島正充, 井上美穂, 小澤彩, 新開瑞希, RAMAN SWARNA LAKSHMI, RAVINDRA PARIMAL CHAVAN, 吉原靖智, 大倉一夫, 鈴木善貴, 松香芳三 :
COVID-19対策が日常となった2021年度歯科補綴学実習の評価,
大学教育カンファレンス in 徳島, 2022年1月. 大倉一夫, 井上美穂, 鈴木善貴, 細木眞紀, 安陪晋, 河野文昭, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの導入とその学習効果 ―オンライン授業とTBL授業の比較―,
日本歯科医学教育学会雑誌, 2021年11月. 細木眞紀, 大倉一夫, 井上美穂, 鈴木善貴, 安陪晋, 河野文昭, 松香芳三 :
コロナ禍におけるデジタル機器およびウェブを利用した歯科補綴学実習の評価,
日本歯科医学教育学会雑誌, 2021年11月. 細木眞紀, Yuehui Zhang, 田島登誉子, 井上美穂, 井上正久, 大島正充, 宮城麻友, 松香芳三 :
モデルマウスによるチタンアレルギーの検討,
日本歯科医学会誌, 2021年9月. 吉原靖智, 鈴木善貴, 大倉一夫, 重本修伺, 安陪晋, 大島正充, 細木眞紀, 井上美穂, 坂東永一, 松香芳三 :
顎運動を伴うRMMAの三次元顎運動解析を行った症例,
日本補綴歯科学会雑誌, 2021年6月. Yuehui Zhang, 細木眞紀, 大島正充, 宮城麻友, 成谷美緒, 井上美穂, 松香芳三 :
金属アレルギー患者群と健常者群における特異的マイクロRNAの同定,
日本補綴歯科学会雑誌, 2021年6月. 大島正充, RESMI RAJU, YAN HUIJIAO, 井上美穂, 松香芳三 :
骨‐歯根膜線維の複合組織立体形成による歯周組織再生技術の開発,
第20回日本再生医療学会総会, 2021年3月. 葉山莉香, 大倉一夫, 大島正充, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂, ロディス Maningo オマー, Junhel Dalanon, Raju Resmi, 松香芳三 :
補綴学授業における反転授業とTBLの学修効果に関する比較,
四国歯学会雑誌, 2021年2月. 大島正充, RESMI RAJU, 閻会嬌, 井上美穂, 松香芳三 :
骨‐歯根膜線維の複合組織立体形成による歯周組織再生技術の開発,
日本再生医療学会, 2021年2月. 細木眞紀, 大倉一夫, 大島正充, 西川泰史, Resmi Raju, 小澤彩, 新開瑞希, Arief Waskitho, Swarna L.Raman, Parimal R.Chavan, 井上美穂, 鈴木善貴, 吉原靖智, 松香芳三 :
歯科補綴学実習における新型コロナウイルス感染症対策について,
大学教育カンファレンス in 徳島, 2021年1月. 大倉一夫, 大島正充, 細木眞紀, 鈴木善貴, 井上美穂, 吉原靖智, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの学修効果―オンライン授業とTBL授業の比較―,
大学教育カンファレンス in 徳島, 2021年1月. 細木眞紀, Yuehui Zhang, 井上美穂, 井上正久, 松香芳三 :
チタンアレルギーモデルマウスの作製と免疫担当細胞の病理組織学的検討,
日本皮膚免疫アレルギー学会, 2020年12月. 細木眞紀, 大倉一夫, 井上美穂, 鈴木善貴, 安陪晋, 河野文昭, 松香芳三 :
新型コロナウイルス感染症対策を踏まえた冠橋義歯学実習の検討,
日本歯科医学教育学会雑誌, 2020年9月. 大倉一夫, 井上美穂, 鈴木善貴, 細木眞紀, 河野文昭, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの導入とその学習効果 ―通常授業，TBL，反転授業の比較―,
日本歯科医学教育学会雑誌, 2020年9月. 大島正充, Resmi Raju, 井上美穂, 宮城麻友, 松香芳三 :
骨‐歯根膜線維の複合組織形成による三次元組織再生技術の開発,
公益社団法人日本補綴歯科学会第129回学術大会, 2020年6月. 岩浅匠真, 井上美穂, Resmi Raju, 大島正充, 松香芳三 :
IL-10は神経障害性疼痛を抑制する,
日本補綴歯科学会雑誌, 2020年6月. 細木眞紀, ZHANG YUEHUI, 大島正充, 井上美穂, 成谷美緒, 宮城麻友, 松香芳三 :
血中マイクロRNAを介した金属アレルギーの感作・発症機序の解明,
日本歯科医学会誌, Vol.39, 58, 2020年3月. 葉山莉香, 大倉一夫, 大島正充, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂, Omar Marianito, 岩浅匠真, 松香芳三 :
補綴学授業におけるアクティブラーニングによる学修効果-反転授業とTBLの長期比較-,
徳島県歯科医学大会, 2020年2月. 岩浅匠真, Shaista Afroz, 井上美穂, 新垣理恵子, 大島正充, Resmi Raju, Arief Waskitho, 井上正久, 馬場麻人, 松香芳三 :
痛みに対するサイトカイン療法の開発に向けて,
徳島県歯科医学大会, 2020年2月. 細木眞紀, 井上美穂, 岩浅匠真, 西川泰史, 秋月皆人, 吉原靖智, 大倉一夫, Yuehui Zhang, Huijiao Yan, Arief Waskitho, Ofuchi R.S., 大島正充, 鈴木善貴, 成谷美緒, 宮城麻友, Resmi Raju, 松香芳三 :
歯科補綴学(Ⅱ)実習における実習書改定後の検討,
大学教育カンファレンスin徳島, 2019年12月. 大倉一夫, 大島正充, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂, 岩浅匠真, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの学修効果―反転授業とTBL，通常授業の比較―,
大学教育カンファレンスin徳島, 2019年12月. Raju Resmi, Masamitsu Ohshima, Miho Inoue, Tsuyoshi Morita, Yan Huijiao, Otto Baba, Inoue Masahisa and Yoshizo Matsuka :
Development of a multilayered complex cell sheet to regenerate periodontal tissue,
Tokushima University Bioscience Retreat, Sep. 2019. Yuehui Zhang, 細木眞紀, 大島正充, 宮城麻友, 成谷美緒, 井上美穂, 松香芳三 :
金属アレルギー患者群と健常者群における特異的マイクロRNAの同定,
日本補綴歯科学会中国・四国支部学術大会, 2019年9月. 成谷美緒, 細木眞紀, 宮城麻友, 大島正充, 井上美穂, 岩浅匠真, 松香芳三 :
金属アレルギー患者に対する血管内治療の術前評価,
日本補綴歯科学会中国・四国支部学術大会, 2019年9月. 細木眞紀, Zhang Yuehui, 大島正充, 井上美穂, 成谷美緒, 宮城麻友, 松香芳三 :
血中マイクロRNAを介した金属アレルギーの感作・発症機序の解明,
歯科医学を中心とした総合的な研究を推進する集い, 2019年8月. Resmi Raju, 大島正充, 井上美穂, 守田剛, Huijiao Yan, 馬場麻人, 井上正久, 松香芳三 :
骨芽細胞と歯根膜細胞との立体形成複合細胞シートによる歯周組織再生の確立,
硬組織再生生物学会, 2019年8月. 大倉一夫, 井上美穂, 細木眞紀, 河野文昭, 松香芳三 :
卒前専門基礎教育におけるアクティブラーニングの導入とその学習効果―反転授業とTBLの比較―,
日本歯科医学教育学会雑誌, 2019年7月. 井上美穂, ラジュレスミ, 岩浅匠真, 秋山謙太郎, 大島正充, 窪木拓男, 松香芳三 :
抗Dickkopf1 (DKK-1) 抗体による骨分化能メカニズムの解明,
日本補綴歯科学会雑誌, 2019年5月. 細木眞紀, 井上美穂, 岩浅匠真, 西川泰史, 秋月皆人, 吉原靖智, 大倉一夫, Zhang Yuehui, Yan Huijiao, Arief Waskitho, Rodrigo Shigeru Ofuchi, 大島正充, 鈴木善貴, 成谷美緒, 宮城麻友, Resmi Raju, 松香芳三 :
歯科補綴学(Ⅱ)実習における実習書改定後の検討in徳島,
大学教育カンファレンス, 2018年12月. 大倉一夫, 大島正充, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂, 岩浅匠真, ジュネルダラノン, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの学修効果-反転授業とTBLの比較-,
大学教育カンファレンスin徳島, 2018年12月. 細木眞紀, 宮城麻友, 井上美穂, 大倉一夫, 大島正充, Resmi Raju, 岩浅匠真, 成谷美緒, ZHANG YUEHUI, 閻会嬌, Arief Waskitho, Dalanon Junhel Casinillo, 鈴木善貴, 吉原靖智, 松香芳三 :
歯科補綴学実習における実習書の改訂とその効果の検討,
大学教育カンファレンスin徳島, 2018年12月. 川原博雄, 井上美穂, 大島正充, 大倉一夫, 細木眞紀, 田島登誉子, 松香芳三 :
一般歯科診療所での長期メインテナンス患者における歯の喪失原因の評価,
日本補綴歯科学会中国・四国支部学術大会, 2018年9月. Raju Resmi, Miho Inoue, 宮城麻友, 秋山謙太郎, Masamitsu Ohshima, 井上正久 and Yoshizo Matsuka :
抗Dickkopf1 (DKK-1) 抗体による骨髄細胞の骨分化能への影響,
硬組織再生生物学会, Aug. 2018. 井上美穂, Resmi Raju, 大島正充, 梶本昇, 都留寛治, 石川邦夫, 井上正久, 松香芳三 :
Tumor necrosis factor (TNF)-α処理炭酸アパタイトおよびハイドロキシアパタイトが骨形成におよぼす影響,
硬組織再生生物学会, 2018年8月. 岩浅匠真, SHAISTA AFROZ, Casinilo Junhel Dalanon, 大島正充, 大倉一夫, 井上美穂, 松香芳三 :
三叉神経痛モデルラットにおける炎症性サイトカインの変動解析,
日本顎関節学会・日本口腔顔面痛学会・日本歯科心身医学会, 2018年7月. 鴨居浩平, 鈴木善貴, 大倉一夫, 宮城麻友, 井上美穂, 大島正充, 清水裕次, 松香芳三 :
ナイトガードの咬耗面を3次元画像解析装置にて定量評価した症例,
四国歯学会雑誌, 2018年3月. 大倉一夫, 西川啓介, 大島正充, 細木眞紀, 鈴木善貴, 宮城麻友, 井上美穂, 岩浅匠真, 葉山莉香, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの効果 ―反転授業とTBLの比較―,
教育カンファレンスin徳島, 2017年12月. S Afroz, Iwasa Takuma, Masamitsu Ohshima, Rieko Arakaki, Miho Inoue, Naozumi Ishimaru and Yoshizo Matsuka :
Cytokine released by satellite glial cells and pain transmission in sensory ganglions,
Japan Association for Dental Research, Nov. 2017. 川原博雄, 井上美穂, 大倉一夫, 大島正充, 松香芳三 :
長期的メインテナンス患者における歯の喪失に関する後ろ向き調査,
日本口腔リハビリテーション学会雑誌, 2017年11月. 岩浅匠真, SHAISTA AFROZ, 成谷美緒, 宮城麻友, 井上美穂, 大島正充, 松香芳三 :
神経障害性痛モデルラットに対するボツリヌス毒素の効果,
日本ボツリヌス治療学会, 2017年9月. RESMI RAJU, 井上美穂, 成谷美緒, 宮城麻友, 織田英正, 友永泰弘, 大島正充, 松香芳三 :
骨髄由来間葉系細胞におけるTNF-α短期刺激による細胞動態の解析,
日本補綴歯科学会中国四国支部学術大会, 2017年8月. 井上美穂, 成谷美緒, RESMI RAJU, 宮城麻友, ZHANG YUEHUI, 大島正充, 井上正久, 松香芳三 :
骨髄細胞，骨芽細胞様細胞におけるTNF-α短期間刺激による細胞挙動の解析,
日本硬組織再生生物学会, 2017年8月. 岩浅匠真, 大島正充, 大倉一夫, 井上美穂, SHAISTA AFROZ, 松香芳三 :
口腔顎顔面痛モデル三叉神経節におけるサイトカイン動態,
日本口腔顔面痛学会雑誌, 2017年7月. 照屋大貴, 井上美穂, 成谷美緒, RESMI RAJU, 宮城麻友, 大島正充, 松香芳三 :
骨髄細胞，骨芽細胞様細胞におけるTNF-α短期刺激による細胞学的・組織学的検討,
日本補綴歯科学会雑誌, 2017年7月. 照屋大貴, 井上美穂, 成谷美緒, RESMI RAJU, 宮城麻友, 大島正充, 松香芳三 :
TNF-α短期刺激による骨髄細胞の細胞学的検討,
四国歯学会雑誌, 2017年6月. 井上美穂, 成谷美緒, RESMI RAJU, 宮城麻友, 松香芳三 :
腫瘍壊死因子TNF-αによる歯髄細胞，骨髄細胞の幹細胞化,
徳島大学大学院医歯薬学研究部発生・再生・遺伝クラスター・ミニリトリート, 2017年3月. 松香芳三, 岩浅匠真, SHAISTA AFROZ, 大本勝弘, 井上美穂, 成谷美緒, 宮城麻友, 大倉一夫 :
末梢知覚神経節における神経伝達物質による痛み情報伝達,
徳島大学医歯薬学研究部脳科学クラスター・ミニリトリート, 2017年3月. 大倉一夫, 西川啓介, 細木眞紀, 田島登誉子, 鈴木善貴, 宮城麻友, 井上美穂, 大本勝弘, 葉山莉香, 松香芳三 :
歯科補綴学授業におけるアクティブラーニングの効果 ―反転授業とTBLの比較―,
大学教育再生加速プログラムシンポジウム, 2016年12月. 藤枝創, 細木眞紀, 西川啓介, 井上美穂, 中川敬司, 鴨居浩平, 大塩恭仁, 宮本裕司, 松香芳三 :
ジルコニア切削方法の違いが強度におよぼす影響について,
日本補綴歯科学会九州,中国・四国支部学術大会, 2016年9月. 宮城麻友, 井上美穂, 福本美緒, 松香芳三 :
炎症環境による歯髄細胞の幹細胞化―歯髄細胞分化に与える TNF-αの影響―,
日本顎口腔機能学会雑誌, 2016年4月. 松香芳三, 大本勝弘, 岩浅匠真, 井上美穂, 福本美緒, 宮城麻友, 大倉一夫 :
知覚神経節における神経伝達物質による痛み情報伝達の可能性,
日本顎口腔機能学会雑誌, 2016年4月.

研究会・報告書: Raju Resmi, Masamitsu Ohshima, Tsuyoshi Morita, Yan Huijiao, Miho Inoue, Otto Baba, Inoue Masahisa and Yoshizo Matsuka :
Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet,
発生・再生・遺伝クラスターミニリトリート, Feb. 2020. Resmi Raju, Miho Inoue, M. Naritani, Mayu Miyagi, Yuehui Zhang, Masamitsu Oshima, Masahisa Inoue and Yoshizo Matsuka :
Cellular kinetics of bone marrow derived mesenchymal cells by short-term TNF-alpha stimulation,
Tokushima University Bioscience Retreat, Sep. 2017. S. Afroz, Iwasa Takuma, Oshima Masamitsu, Rieko Arakaki, Miho Inoue, Naozumi Ishimaru and Yoshizo Matsuka :
Cytokine release from satellite glial cells of trigeminal ganglia,
Tokushima University Bioscience Retreat, Sep. 2017.

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 口腔顔面痛モデル動物の精油の嗅覚刺激による痛み刺激の軽減と脳神経メカニズムの解明 (研究課題/領域番号: 22K10060 )
microRNAを用いた金属アレルギーによる口腔扁平苔癬の新規診断マーカーの確立 (研究課題/領域番号: 21K10022 )
末梢血miRNA発現解析による金属アレルギー疾患の新規診断バイオマーカーの確立 (研究課題/領域番号: 20K10038 )
咬合メカニカルストレスによる歯周組織形成・成熟機構に立脚したバイオ人工歯根の開発 (研究課題/領域番号: 19K10208 )
骨形成作用を有する抗DKK-1抗体の効果メカニズムの解明 (研究課題/領域番号: 17K11753 )
歯髄細胞の幹細胞化を応用した新規治療法の開発 (研究課題/領域番号: 16K11600 )
金属アレルギー；感作と発症間に何があるのか？―特異的遺伝子の同定― (研究課題/領域番号: 16K11599 )
歯髄・象牙質再生を促進する新規生体材料の開発 (研究課題/領域番号: 15H06450 )
分子生物学的アプローチによる口腔内細菌叢の動態調査 (研究課題/領域番号: 10671699 )
新規軟骨由来血管新生因子(CTGF)に対する阻害剤の開発と血管新生病治療への応用 (研究課題/領域番号: 10557165 )
軟骨肉腫由来軟骨成長因子エコジェニン/CTGFのレセプターの分子クローニング (研究課題/領域番号: 09671893 )
Helicobacter pyroliの口腔内における生態解析 (研究課題/領域番号: 08672069 )
若年性歯周病原性細菌が産生する白血球毒素蛋白質の機能領域の同定 (研究課題/領域番号: 07771611 )
ミュータンス菌のグルカン合成酵素の構造と機能 (研究課題/領域番号: 06671811 )
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2025年4月25日更新

専門分野・研究分野: 骨ミネラル代謝学 (Bone and Mineral Metabolism)
生体材料学 (Biomaterials)
歯科補綴学 (Prosthodontics)
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受賞: 2018年8月, 優秀発表賞 (硬組織再生生物学会)
2019年8月, Poster presentation Award (硬組織再生生物学会)
2019年9月, 優秀発表賞 (歯科医学を中心とした総合的な研究を推進する集い)
2020年6月, 学術大会課題口演賞 (日本補綴歯科学会)
2022年7月, 学術大会優秀ポスター賞 (日本補綴歯科学会)
2023年5月, 学術大会優秀ポスター賞 (日本補綴歯科学会)
2024年11月, 菊池賞 (日本睡眠歯科学会)
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2025年4月19日更新

研究者番号: 20271059

所属（現在）: 2025/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 助教

所属（過去の研究課題 情報に基づく）*注記: 2018/4/1 – 2023/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学域), 助教
2016/4/1 – 2017/4/1 : 徳島大学, 大学院医歯薬学研究部(歯学系), 助教
2015/4/1 : 徳島大学, 大学病院, 診療支援医師
1995/4/1 – 1999/4/1 : 岡山大学, 歯学部, 教務員

審査区分/研究分野

研究代表者

医学 / 歯学 / 形態系基礎歯科学
生物系 / 医歯薬学 / 歯学 / 補綴・理工系歯学
小区分57050:補綴系歯学関連

研究代表者以外

医学 / 歯学 / 形態系基礎歯科学
医学 / 歯学 / 機能系基礎歯科学
生物系 / 医歯薬学 / 歯学 / 補綴・理工系歯学
小区分57050:補綴系歯学関連

キーワード

研究代表者

Actinobacillus actinomycetemcomitans / 白血球毒素 / 蛋白質リン酸化 / 幹細胞化 / 骨芽細胞様細胞 / 骨髄細胞 / 骨芽細胞 / 分化 / 歯学 / 生体材料 / 抗DKK-1抗体 / 骨形成 / 骨代謝 / 骨粗しょう症 / TNF-α / 骨形成能 / 骨粗しょう症モデルマウス / 顎骨壊死 / シグナル伝達 / 再生・医学 / 柑橘系精油 / 痛み / 口腔顔面痛モデル / 三叉神経節 / サイトカイン / ベルガモット

研究代表者以外

Streptococcus mutans / Glucosyltransferase / 不溶性グルカン / グルカン合成酵素 / GTF / GTF遺伝子 / glucosyltransferase / streptococcus mutans / グルカン合成酵素遺伝子 / Streptococcus sobrinus / function asd structure / water-insoluble glucan / alpha-1,3 glucan / dextran-dependent / ヘリコバクター・ピロリ / 胃潰瘍・十二指腸潰瘍 / 球状体 / ケモスタット / Helicobacter pylori / 口腔細菌 / 生態学 / PCR / gastric ulcer / duodenal ulcer / morphologic conversion / chemostat culture / differential display / 結合組織成長因子(CTGF) / クロスリンク / アフィニティークロマト / MAPキナーゼ / プロテオグリカン / インテグリン / チロシンキナーゼ / CTGF / レセプター / 内軟骨性骨化 / ペプチドマッピング / 軟骨細胞 / 結合組織増殖因子(CTGF) / Scatchard解析 / crosslink / PDGF / FGF / connective tissue growth factor (CTGF) / affinity chromatography / MAP kinase / proteoglycan / integrin / tyrosine kinase / 血管新生 / 結合組織成長因子 / 抗CTGF / 血管内皮細胞 / 転移 / 慢性関節リウマチ / 心筋梗塞 / 抗CTGF抗体 / angiogenesis / anti-CTGF antibody / endothelial cells / metastasis / rheumatoid arthritis / neovascularization / myocardial infarction / 細菌叢 / 16SrDNA / う蝕 / 歯周病原細菌 / 齲蝕 / genotype / 16S rRNA / bacterial community / dental caries / periodontal bacteria / 金属アレルギー / マイクロRNA / パッチテスト / microRNA / miRNA / チタン / ピアス / 歯科補綴学一般 / アレルギー / 歯髄細胞 / TNF-α / 幹細胞 / 幹細胞化 / 歯科インプラント / 歯周組織 / メカニカルストレス / 歯科用インプラント / アレルギー性接触皮膚炎 / バイオマーカー / 扁平苔癬 / マイクロアレイ解析 / 歯科補綴学

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井上 美穂

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