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赤松徹也

徳島大学

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2024年4月19日更新

職名: 准教授
電話: 088-633-7324
電子メール: akamatsu@dent.tokushima-u.ac.jp
学歴: 1992/3: 徳島大学工学部生物工学科卒業
1994/3: 徳島大学大学院工学研究科生物工学専攻修了
1997/3: 徳島大学大学院工学研究科物質工学専攻単位取得退学
1997/9: 徳島大学大学院工学研究科博士後期課程修了
学位: 博士(工学) (徳島大学) (1997年9月)
職歴・経歴: 1997/4: 徳島大学歯学部口腔生理学講座助手
2004/4: 徳島大学大学院ヘルスバイオサイエンス研究部生体システム栄養科学部門摂食機能制御学講座口腔分子生理学分野助手
2007/4: 徳島大学大学院ヘルスバイオサイエンス研究部口腔分子生理学分野助教
2009/4: 徳島大学大学院ヘルスバイオサイエンス研究部口腔分子生理学分野講師
2010/4: 徳島大学大学院ヘルスバイオサイエンス研究部口腔分子生理学分野准教授
2015/4: 徳島大学大学院医歯薬学研究部口腔分子生理学分野准教授
2016/4: 徳島大学大学院生物資源産業学研究部生体分子機能学分野准教授
2017/4: 徳島大学大学院社会産業理工学研究部生体分子機能学分野准教授

専門分野・研究分野: 生化学 (Biochemistry)
細胞生理学 (Cell Physiology)
分子細胞生物学 (Molecular Cell Biology)
発生生物学 (Developmental Biology)

研究者総覧で最新データを確認する

2024年4月19日更新

専門分野・研究分野: 生化学 (Biochemistry)
細胞生理学 (Cell Physiology)
分子細胞生物学 (Molecular Cell Biology)
発生生物学 (Developmental Biology)
担当経験のある授業科目: フードサイエンス (学部)
卒業研究 (学部)
味覚サイエンス (学部)
基礎生理学 (学部)
基礎食品化学 (学部)
栄養・口腔生理学 (学部)
栄養生理学 (学部)
生体機能学特論 (大学院)
生物資源学研究 (大学院)
生物資源産業学B (学部)
生物資源産業学実習 (学部)
生物資源産業学専門英語 (学部)
英語論文講読 (学部)
英語論文講読Ⅰ (学部)
英語論文講読Ⅱ (学部)
食と健康概論 (学部)
食料生物科学特別実習 (大学院)
食料生物科学特別演習 (大学院)
食料生物科学特別研究 (大学院)
食料生物科学特別講義 (大学院)
食料科学基礎実習 (学部)
食料科学実習A (学部)
食料科学実習B (学部)
食料科学実習C (学部)
食料科学実習Ⅰ (学部)
食料科学実習Ⅱ (学部)
食料科学概論 (学部)
指導経験: 8人 (学士), 6人 (修士)

研究者総覧で最新データを確認する

2024年4月19日更新

専門分野・研究分野: 生化学 (Biochemistry)
細胞生理学 (Cell Physiology)
分子細胞生物学 (Molecular Cell Biology)
発生生物学 (Developmental Biology)

研究テーマ: 水チャネル, アクアポリンに関する研究, プロセシング酵素に関する研究, 口腔組織発生に関する研究 (アクアポリン, 水チャネル, プロセシング酵素, 前駆体蛋白質変換酵素, 口腔組織, 発生 (development))

著書

赤松徹也, 姚陳娟, 細井和雄 :
動物細胞培養の手法と細胞死・増殖不良・細胞変異を防止する技術,
株式会社技術情報協会, 2014年4月.

(キーワード): 唾液腺 (salivary gland) / 組織培養 / 分化誘導

Tetsuya Akamatsu and al. et :
Handbook of Proteolytic Enzymes. 3rd Ed., --- Proprotein Convertase PACE4. ---,
Academic Press, Dec. 2012. 細井和雄, 赤松徹也, 他 :
口腔生物学各論-唾液腺,
学建書院, 東京, 2006年2月. 細井和雄, 樋口富彦, 柏俣正典, 新垣理恵子, 新垣尚捷, 多田淳, 津村恵子, 赤松徹也, 武田京子, 上野明道, 末永みどり, 馬場嘉信, 他 :
分子細胞生物学基礎実験法改訂第2版(分担),
南江堂, 東京, 2004年4月.

(キーワード): In situ hybridization

Hideaki Nagamune, Kazuaki Muramatsu, Masakatsu Higashine, Tetsuya Akamatsu, Yasuhiro Tamai, Yasuo Yonetomi, Akihiko Tsuji, Keisuke Izumi, Takemasa Sakaguchi, Tetsuya Yoshida and Yoshiko Matsuda :
Cyclic AMP-inducible procalcitonin processing in thyroidal parafollicular cells is regulated by the Kexin family protease, PC1.,
IOS Press, Amsterdam, Mar. 1997.

(要約): カルシウム調節ホルモンであるカルシトニンは甲状腺傍濾胞細胞より分泌されるが, 不活性な前駆体として合成された後, プロセシングを受けて初めて活性化される. 本研究ではcAMP刺激により著増するカルシトニンのプロセシングがKexin family proteaseに属する酵素PC1により調節されることを初めて明らかにした.
(キーワード): Calcitonin / Processing / PC1

論文

Shintaroh Kusunoki, Takako Fukuda, Saori Maeda, Chenjuan Yao, Takahiro Hasegawa, Tetsuya Akamatsu and Hiroshi Yoshimura :
Relationships between feeding behaviors and emotions: An electroencephalogram (EEG) frequency analysis study,
The Journal of Physiological Sciences, Vol.73, No.1, 2, 2022.

(要約): Feeding behaviors may be easily affected by emotions, both being based on brain activity; however, the relationships between them have not been explicitly defined. In this study, we investigated how emotional environments modulate subjective feelings, brain activity, and feeding behaviors. Electroencephalogram (EEG) recordings were obtained from healthy participants in conditions of virtual comfortable space (CS) and uncomfortable space (UCS) while eating chocolate, and the times required for eating it were measured. We found that the more participants tended to feel comfortable under the CS, the more it took time to eat in the UCS. However, the EEG emergence patterns in the two virtual spaces varied across the individuals. Upon focusing on the theta and low-beta bands, the strength of the mental condition and eating times were found to be guided by these frequency bands. The results determined that the theta and low-beta bands are likely important and relevant waves for feeding behaviors under emotional circumstances, following alterations in mental conditions.
(キーワード): 摂食行動 / 情動 / 脳波 (electroencephalogram) / 高速フーリエ変換 / 仮想空間
(徳島大学機関リポジトリ): ● Metadata: 118602
(出版サイトへのリンク): ● Publication site (DOI): 10.1186/s12576-022-00858-w
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 36869303; ● Search Scopus @ Elsevier (PMID): 36869303; ● Search Scopus @ Elsevier (DOI): 10.1186/s12576-022-00858-w

(徳島大学機関リポジトリ: 118602, DOI: 10.1186/s12576-022-00858-w, PubMed: 36869303) SNM Hashim, MFH Yusof, W Zahari, KBAA Noordin, Tetsuya Akamatsu and A Azlina :
Amniotic membrane matrix effects on calcineurin-NFAT-related gene expressions of SHED treated with VEGF for endothelial differentiation.,
In Vitro Cellular & Developmental Biology. Animal, Vol.57, No.5, 560--570, 2021.

(要約): The nuclear factor of activated T-cell (NFAT) signaling pathway is involved in angiogenesis following initiation by vascular endothelial growth factor (VEGF). A number of angiogenic genes have been associated with calcineurin in the NFAT pathway, forming a calcineurin-NFAT pathway. This study aims to investigate the involvement of four angiogenic genes within the calcineurin-NFAT pathway in the endothelial-like differentiation of stem cells from human exfoliated deciduous teeth (SHED) cultured on a human amniotic membrane (HAM) induced by VEGF. SHED were induced with VEGF for 24 h, then cultured on the stromal side of HAM. The cells were then further induced with VEGF until days 1 and 14. To understand the role of calcineurin, its potent inhibitor, cyclosporin A (CsA), was added into the culture. Results from SEM and H&E analyses showed SHED grew on HAM surface. Gene expression study of Cox-2 showed a drastically reduced expression with CsA treatment indicating Cox-2 involvement in the calcineurin-NFAT pathway. Meanwhile, IL-8 was probably controlled by another pathway as it showed no CsA inhibition. In contrast, high expression of ICAM-1 and RCAN1.4 by VEGF and CsA implied that these genes were not controlled by the calcineurin-NFAT-dependent pathway. In conclusion, the results of this study suggest the involvement of Cox-2 in the calcineurin-NFAT-dependent pathway while RCAN1.4 was controlled by NFAT molecule in endothelial-like differentiation of SHED cultured on HAM with VEGF induction.
(キーワード): Amnion / Calcineurin / Cell Differentiation / Cells, Cultured / Endothelium / Gene Expression Regulation / Humans / NFATC Transcription Factors / Real-Time Polymerase Chain Reaction / Stem Cells / Tooth, Deciduous / Vascular Endothelial Growth Factor A
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s11626-021-00588-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 34021476; ● Search Scopus @ Elsevier (PMID): 34021476; ● Search Scopus @ Elsevier (DOI): 10.1007/s11626-021-00588-0

(DOI: 10.1007/s11626-021-00588-0, PubMed: 34021476) Sayaka Doi, Mina Kawamura, Keisuke Oyama, Tetsuya Akamatsu, Mizuki Mizobuchi, Yasuo Oyama, Toshiya Masuda and Norio Kamemura :
Bioactivity of alginetin, a caramelization product of pectin: Cytometric analysis of rat thymic lymphocytes using fluorescent probes.,
PLoS ONE, Vol.15, No.11, e0241290, 2020.

(要約): Alginetin is the major product formed from pentoses and hexurionic acids. Alginetin is producted by cooking process of food including pection, a naturally-occurring polysacharride found in many plants. However, the biological interaction and toxicity of alginetin are not known at all. The aim of the present study was to investigate the cellular actions of alginetin on rat thymic lymphocytes. The effects of alginetin on the cell were examined using flow cytometry with fluorescent probes. Alginetin increased cellular content of non-protein thiols ([NPT]i) and elevated intracellular Zn2+ levels ([Zn2+]i). Chelation of intracellular Zn2+ reduced the effect of alginetin on [NPT]i, and chelation of external Zn2+ almost completely diminished alginetin-induced elevation of [Zn2+]i, indicating that alginetin treatment increased Zn2+ influx. Increased [NPT]i and [Zn2+]i levels in response to alginetin were positively correlated. Alginetin protected cells against oxidative stress induced by hydrogen peroxide and Ca2+ overload by calcium ionophore. It is considered that the increases in [NPT]i and [Zn2+]i are responsible for the cytoprotective activity of alginetin because NPT attenuates oxidative stress and Zn2+ competes with Ca2+. Alginetin may be produced during manufacturing of jam, which may provide additional health benefits of jam.
(徳島大学機関リポジトリ): ● Metadata: 116621
(出版サイトへのリンク): ● Publication site (DOI): 10.1371/journal.pone.0241290
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33137129; ● Search Scopus @ Elsevier (PMID): 33137129; ● Search Scopus @ Elsevier (DOI): 10.1371/journal.pone.0241290

(徳島大学機関リポジトリ: 116621, DOI: 10.1371/journal.pone.0241290, PubMed: 33137129) Shogo Abe, Misako Ueno, Mami Nishitani, Tetsuya Akamatsu, Takumi Sato, Marie Shimoda, Hiroki Kanaoka, Yoshitaka Nii, Hiroko Yamasaki and Keizo Yuasa :
Citrus sudachi Peel Extract Suppresses Cell Proliferation and Promotes the Differentiation of Keratinocytes through Inhibition of the EGFR-ERK Signaling Pathway.,
Biomolecules, Vol.10, No.10, 1468, 2020.

(徳島大学機関リポジトリ): ● Metadata: 116486
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/biom10101468
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 33096942; ● Search Scopus @ Elsevier (PMID): 33096942; ● Search Scopus @ Elsevier (DOI): 10.3390/biom10101468

(徳島大学機関リポジトリ: 116486, DOI: 10.3390/biom10101468, PubMed: 33096942) Hiroshi Yoshimura, Sugai Tokio, Kato Nobuo, Tominaga Takashi, Tominaga Yoko, Takahiro Hasegawa, Chenjuan Yao and Tetsuya Akamatsu :
Interplay between non-NMDA and NMDA receptor activation during oscillatory wave propagation: Analyses of caffeine-induced oscillations in the visual cortex of rats,
Neural Networks, Vol.79, 141-149, 2016.

(要約): Generation and propagation of oscillatory activities in cortical networks are important features of the brain. However, many issues related to oscillatory phenomena are unclear. We previously reported neocortical oscillation following caffeine treatment of rat brain slices. Input to the primary visual cortex (Oc1) generates N-methyl-d-aspartate (NMDA) receptor-dependent oscillations, and we proposed that the oscillatory signals originate in the secondary visual cortex (Oc2). Because non-NMDA and NMDA receptors cooperate in synaptic transmission, non-NMDA receptors may also play an important role in oscillatory activities. Here we investigated how non-NMDA receptor activities contribute to NMDA receptor-dependent oscillations by using optical recording methods. After induction of stable oscillations with caffeine application, blockade of NMDA receptors abolished the late stable oscillatory phase, but elicited 'hidden' non-NMDA receptor-dependent oscillation during the early depolarizing phase. An interesting finding is that the origin of the non-NMDA receptor-dependent oscillation moved from the Oc1, during the early phase, toward the origin of the NMDA receptor-dependent oscillation that is fixed in the Oc2. In addition, the frequency of the non-NMDA receptor-dependent oscillation was higher than that of the NMDA receptor-dependent oscillation. Thus, in one course of spatiotemporal oscillatory activities, the relative balance in receptor activities between non-NMDA and NMDA receptors gradually changes, and this may be due to the different kinetics of the two receptor types. These results suggest that interplay between the two receptor types in the areas of Oc1 and Oc2 may play an important role in oscillatory signal communication.
(キーワード): neural oscillation / non-NMDA receptor / NMDA受容体 (NMDA-receptors) / caffeine / ラット (rat)
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.neunet.2016.03.012
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 27136667; ● Search Scopus @ Elsevier (PMID): 27136667; ● Search Scopus @ Elsevier (DOI): 10.1016/j.neunet.2016.03.012

(DOI: 10.1016/j.neunet.2016.03.012, PubMed: 27136667) Hiroshi Yoshimura, 川邊真道, 須貝外喜夫, 加藤伸郎, Takahiro Hasegawa, Chenjuan Yao and Tetsuya Akamatsu :
Influences of oral impairment on neural oscilltion and wave propagation in the neocortex of rats,
Neuroscience Research Suppl, Vol.Suppl, 2015. Gang Chen, Chenjuan Yao, Takahiro Hasegawa, Tetsuya Akamatsu, Hiroshi Yoshimura and Kazuo Hosoi :
Effects of isoproterenol on aquaporin 5 levels in the parotid gland of mice in vivo.,
American Journal of Physiology, Endocrinology and Metabolism, Vol.306, No.1, E100-E108, 2014.

(要約): In the membrane fraction of mouse parotid gland (PG), the protein level of aquaporin 5 (AQP5), a member of the water channel family, was increased by injection (ip) of isoproterenol (IPR), a β-adrenergic agonist, at 1 h, and stayed at high levels until 6 h; this change occurred simultaneously as amylase secretion. The AQP5 level then decreased and returned toward the original level at 12-48 h. After IPR injection, the AQP5 mRNA gradually increased and reached a maximum at 24 h. The facts suggest a rapid appearance of AQP5 at plasma membrane by IPR and subsequent degradation/metabolism by activation of proteolytic systems. Pretreatment of animals with two calpain inhibitors, N-Ac-Leu-Leu-methininal (ALLM) and calpeptin, as well as a protein synthesis inhibitor, cycloheximide (CHX), significantly suppressed the IPR-induced AQP5 degradation in the PG membrane fraction; such suppression was not observed by two proteasome inhibitors, MG132 and lactacystin, or the lysosome denaturant chloroquine, although most of these inhibitors increased AQP5 protein levels in unstimulated mice. The AQP5 protein was also degraded by μ-calpain in vitro. Furthermore, we demonstrated that μ-calpain was colocalized with AQP5 in the acinar cells by immunohistochemistry, and its activity in the PG was increased at 6 h after IPR injection. These results suggest that the calpain system was responsible for IPR-induced AQP5 degradation in the parotid gland and that such a system was coupled to the secretory-restoration cycle of amylase in the PG.
(キーワード): アクアポリン5 / 耳下腺 / イソプロテレノール
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpendo.00317.2013
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 24192288; ● Summary page in Scopus @ Elsevier: 2-s2.0-84891527146

(DOI: 10.1152/ajpendo.00317.2013, PubMed: 24192288, Elsevier: Scopus) Hiroshi Yoshimura, Tokio Sugai, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Nobuo Kato :
Age-dependent emergence of caffeine-assisted voltage oscillations in the endopiriform nucleus of rats.,
Neuroscience Research, Vol.76, No.1-2, 16-21, 2013.

(要約): The gustatory insular cortex (IC) is connected with not only the somatosensory cortex, but also the endopiriform nucleus (EPN). We have previously revealed that low-frequency electrical stimulation to the IC can elicit membrane potential oscillations at a frequency of 8-10 Hz in the somatosensory cortex of rat brain slices under bath-application of caffeine. Using the same procedure, we investigated whether the EPN has the ability to generate oscillations, and whether such oscillations emerge age-dependently. Electrical stimulations were delivered to the IC, and field potentials were recorded from the EPN. In the case of slices made from mature rats, stable field potential oscillations at 8-10 Hz were induced in the EPN after repetitive stimulations. Optical recordings revealed that signals traveled from the IC to the EPN by way of the claustrum. Generation of oscillations was N-methyl-d-aspartate (NMDA) receptor activity-dependent, since oscillatory phases disappeared following application of NMDA receptor antagonist. In slices from immature rats, however, oscillations were not induced. IC stimulation can thus age-dependently elicit membrane potential oscillations in the EPN, and the EPN oscillations were NMDA receptor activity-dependent. These findings suggest that developmental changes in properties of the EPN might contribute to development of information integration, including gustatory information.
(キーワード): 加齢 (aging) / Animals / Caffeine / Central Nervous System Stimulants / Cerebral Cortex / Electric Stimulation / Membrane Potentials / Organ Culture Techniques / Rats / Rats, Wistar
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.neures.2013.02.010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 23517711; ● Summary page in Scopus @ Elsevier: 2-s2.0-84884702568

(DOI: 10.1016/j.neures.2013.02.010, PubMed: 23517711, Elsevier: Scopus) Nunuk Purwanti, Mileva Ratko Karabasil, Shinsuke Matsuo, Gang Chen, Javkhlan Purevjav, Ahmad Azlina, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Induction of Sca-1 via activation of STAT3 system in the duct cells of the mouse submandibular gland by ligation of the main excretory duct,
American Journal of Physiology, Gastrointestinal and Liver Physiology, Vol.301, No.5, G814-G824, 2011.

(要約): To examine the very initial step that takes place immediately after tissue injury and is linked to tissue regeneration, we employed the submandibular gland (SMG), which was injured by ligation of its main excretory duct (MED). Ligation of the MED of the SMG in mice induced the expression of Sca-1, a protein marker of hematopoietic stem cells. In the normal gland, a low level of Sca-1 was expressed, which was localized predominantly in the excretory duct cells. At 1 day after ligation, Sca-1 expression increased prominently in almost all of cells in the duct system, but not in the acinar cells. The level of Sca-1 mRNA had begun to increase at 6 h after ligation and continuously rose thereafter until it reached a plateau, which occurred ∼12 h after ligation. STAT3 phosphorylated at its tyrosine-705 (p-STAT3) in the ligated gland increased immediately after ligation, and it was localized in the nuclei of all duct cells. The results of an EMSA revealed the specific binding of a nuclear extract to the sequence of the γ-interferon activation site (GAS) present in the Sca-1 promoter and confirmed that such binding increased after ligation. Thus the present study suggests that STAT3, having been phosphorylated following MED ligation, was transferred to the nucleus, where it bound to the GAS element in the promoter of Sca-1 gene, resulting in promotion of Sca-1 gene expression. Actual prevention of STAT3 phosphorylation reduced the ligation-induced Sca-1 elevation.
(キーワード): Animals / Antigens, Ly / Ligation / Membrane Proteins / Mice / Phosphorylation / Promoter Regions, Genetic / STAT3 Transcription Factor / Salivary Ducts / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpgi.00408.2010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21868636; ● Search Scopus @ Elsevier (PMID): 21868636; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpgi.00408.2010

(DOI: 10.1152/ajpgi.00408.2010, PubMed: 21868636) Purevjav Javkhlan, Yuka Hiroshima, Ahmad Azlina, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Jun-ichi Kido, Toshihiko Nagata and Kazuo Hosoi :
Lipopolysaccharide-mediated induction of calprotectin in the submandibular and parotid glands of mice,
Inflammation, Vol.34, No.6, 668-680, 2011.

(要約): S100A8 and S100A9 constitute a heterodimeric protein, calprotectin. The mRNAs of S100A8 and S100A9, being expressed at minimal levels in the submandibular and parotid glands (SMG and PG, respectively) of C3H/HeN mice, were induced strongly and transiently by lipopolysaccharide (LPS). Among the mRNAs of members of the S100 protein family examined, those of S100A8 and S100A9 were specifically induced by LPS in the salivary glands. The induction was assumed to be mediated via toll-like receptor 4 (TLR4), since their elevation was limited in C3H/HeJ mice, a TLR4-mutant strain. These proteins became expressed in the granular convoluted tubular cells and striated duct cells in the SMG, and in both acinar and duct cells in the PG (all in the cytoplasm). The salivary calprotectin level was not increased by LPS treatment, implying that elevated calprotectin was not secreted into the saliva and that they may function in microcellular environment of the salivary gland.
(キーワード): Animals / Calgranulin A / Calgranulin B / Leukocyte L1 Antigen Complex / Lipopolysaccharides / Mice / Parotid Gland / RNA, Messenger / Salivary Glands / Submandibular Gland / Transcriptional Activation
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s10753-010-9277-1
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21125321; ● Search Scopus @ Elsevier (PMID): 21125321; ● Search Scopus @ Elsevier (DOI): 10.1007/s10753-010-9277-1

(DOI: 10.1007/s10753-010-9277-1, PubMed: 21125321) Takahiro Hasegawa, Ahmad Azlina, Javkhlan Purevjav, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Novel phosphorylation of aquaporin-5 at its threonine 259 through cAMP signaling,
American Journal of Physiology, Cell Physiology, Vol.301, No.3, C667-C678, 2011.

(要約): Aquaporin-5 (AQP5), a water channel, plays key roles in salivary secretion. The novel phosphorylation of AQP5 was investigated by using human salivary gland (HSG) cells and mouse salivary glands. In the HSG cells stably transfected with a wild-type mouse AQP5 construct, a protein band immunoreactive with antibody against phosphorylated PKA substrate was detected in the AQP5 immunoprecipitated sample, and its intensity was enhanced by short-term treatment of the cells with 8-bromo-cAMP, forskolin, or phorbol 12-myristate 13-acetate, but not by that with A23187 calcium ionophore. Such enhancement was inhibited in the presence of H-89, a PKA inhibitor. An AQP5 mutant (AQP5-T259A) expressed by transfection of HSG cells was not recognized by anti-phosphorylated PKA substrate antibody, even when the cells were stimulated with the protein kinase activators. Immunoblotting and immunofluorescence studies using a specific antibody detecting AQP5 phosphorylated at its Thr259 demonstrated that AQP5 was rapidly and transiently phosphorylated at the apical membrane of acinar cells in the submandibular and parotid glands after administration of isoproterenol, but not pilocarpine. Furthermore, both AQP5 and AQP5-T259A were constitutively localized at the plasma membrane in HSG cells under the resting and forskolin-stimulated conditions. These results suggest that AQP5 is phosphorylated at its Thr259 by PKA through cAMP, but not Ca(2+), signaling pathways, and that this phosphorylation does not contribute to AQP5 trafficking in the salivary gland cells.
(キーワード): Amino Acid Substitution / Animals / Antibodies / Antibody Specificity / Aquaporin 5 / Calcimycin / Cell Line, Tumor / Cell Membrane / Cyclic AMP / Cyclic AMP-Dependent Protein Kinases / Detergents / Forskolin / Humans / Isoproterenol / Isoquinolines / Male / Mice / Mice, Inbred ICR / Parotid Gland / Phosphorylation / Pilocarpine / Protein Kinase Inhibitors / Protein Transport / Salivary Glands / Signal Transduction / Submandibular Gland / Sulfonamides / Tetradecanoylphorbol Acetate / Threonine / Transfection
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpcell.00058.2011
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21633078; ● Search Scopus @ Elsevier (PMID): 21633078; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpcell.00058.2011

(DOI: 10.1152/ajpcell.00058.2011, PubMed: 21633078) Nunuk Purwanti, Daisuke Tsuji, Ahmad Azlina, Mileva Ratko Karabasil, Purevjav Javkhlan, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Kouji Itou and Kazuo Hosoi :
Induction of Sca-1 in the duct cells of the mouse submandibular gland by obstruction of the main excretory duct,
Journal of Oral Pathology & Medicine, Vol.40, No.8, 651-658, 2011.

(要約): The effect of ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) on the expression of Sca-1, a stem cell antigen, was examined by Western blotting and immunohistochemistry. By Western blotting, the expression of Sca-1 with a molecular weight of 18 kDa was identified in the normal gland. At 1 day post-ligation, the expression level of Sca-1 was strongly increased in the experimental gland and weakly in the contralateral gland, and such expression in both glands decreased at 6 days. By immunohistochemistry, Sca-1 was detected weakly in the apical membrane of excretory duct (ED) cells of the SMG under the normal condition. By duct ligation, Sca-1 became expressed strongly in most cells of the two major duct systems, i.e., the striated duct (SD) and granular convoluted tubules (GCT), but was not detected in the acinar (Ac) cells. By fluorescence-activated cell sorter (FACS) analysis, the number of side population (SP) cells in this gland was found to be increased by ligation. These results imply that Sca-1-positive cells may have a role in the duct cell proliferation in the regeneration step elicited by MED ligation-induced injury.
(キーワード): Animals / Antigens, Ly / Cell Count / Cell Proliferation / Ligation / Male / Membrane Proteins / Mice / Mice, Inbred C57BL / Regeneration / Salivary Ducts / Salivation / Side-Population Cells / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1600-0714.2011.01011.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21884259; ● Search Scopus @ Elsevier (PMID): 21884259; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1600-0714.2011.01011.x

(DOI: 10.1111/j.1600-0714.2011.01011.x, PubMed: 21884259) Mileva Ratko Karabasil, Takahiro Hasegawa, Ahmad Azlina, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Shigemasa Tomioka and Kazuo Hosoi :
Effects of naturally occurring G103D point mutation of AQP5 on its water permeability, trafficking, and cellular localization in the submandibular gland of rats,
Biology of the Cell, Vol.103, No.2, 69-86, 2011.

(要約): AQPs (aquaporins) are water channel proteins that are expressed in almost all living things. In mammalians, 13 members of AQPs (AQP0-12) have been identified so far. AQP5 is known to be expressed mostly in the exocrine cells, including the salivary gland acinar cells. A naturally occurring point mutation (G308A, Gly103 > Asp103) was earlier found in the rat AQP5 gene [Murdiastuti, Purwanti, Karabasil, Li, Yao, Akamatsu, Kanamori and Hosoi (2006) Am. J. Physiol. 291, G1081-G1088]; in this mutant, the rate of initial saliva secretion under stimulated and unstimulated conditions is less than that for the wt (wild-type) animals. Here the mutant molecule was characterized in detail. Using the Xenopus oocyte system, we demonstrated the mutant AQP5 to have water permeability almost the same as that of the wt molecule. Mutant and wt AQP5s, tagged with GFP (green fluorescent protein; GFP-AQP5s) and expressed in polarized MDCK-II (Madin-Darby canine kidney II) cells, first appeared in the vesicular structure(s) in the cytoplasm, and were translocated to the upper plasma membrane or apical membrane during cultivation, with the mutant GFP-AQP5 being translocated less efficiently. Thapsigargin and H-89 both induced translocation in vitro of either molecule, whereas colchicine inhibited this activity; the fraction of cells showing apical localization of mutant GFP-AQP5 was less than that showing that of the wt molecule under any of the experimental conditions used. In the mutant SMG (submandibular gland) tissue, localization of AQP5 in the apical membrane of acinar cells was extremely reduced. Vesicular structures positive for AQP5 and present in the cytoplasm of the acinar cells were co-localized with LAMP2 (lysosome-associated membrane protein 2) or cathepsin D in the mutant gland, whereas such co-localizations were very rare in the wt gland, suggesting that the mutant molecules largely entered lysosomes for degradation. Replacement of highly conserved hydrophobic Gly103 with strongly hydrophilic Asp103 in rat AQP5, though it did not affect water permeability, may possibly have resulted in less efficient membrane trafficking and increased lysosomal degradation, leading to its lower expression in the apical membrane of the acinar cells in the SMG.
(キーワード): Amino Acid Sequence / Animals / Aquaporin 5 / Cell Line / Cell Membrane / Dogs / Molecular Sequence Data / Mutation, Missense / Permeability / Point Mutation / Protein Transport / Rats / Sequence Alignment / Submandibular Gland / Water / Xenopus
(出版サイトへのリンク): ● Publication site (DOI): 10.1042/BC20100086
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 21138418; ● Search Scopus @ Elsevier (PMID): 21138418; ● Search Scopus @ Elsevier (DOI): 10.1042/BC20100086

(DOI: 10.1042/BC20100086, PubMed: 21138418) Ahmad Azlina, Purevjav Javkhlan, Yuka Hiroshima, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Roles of lysosomal proteolytic systems in AQP5 degradation in the submandibular gland of rats following chorda tympani parasympathetic denervation,
American Journal of Physiology, Gastrointestinal and Liver Physiology, Vol.299, No.5, G1106-G1117, 2010.

(要約): Chorda tympani denervation (CTD) of rats was earlier shown to result in loss of submandibular gland (SMG) weight (at only 1 wk) and in continued reduction in aquaporin 5 (AQP5) protein expression (until 4 wk), without affecting its mRNA synthesis (Li X, Azlina A, Karabasil MR, Purwanti N, Hasegawa T, Yao C, Akamatsu T, Hosoi K. Am J Physiol Gastrointest Liver Physiol 295: G112-G123, 2008). The present study indicated that despite elevation of bax, a proapoptosis protein, by CTD, the operation also increased the level of bcl-2, an antiapoptosis protein, in the SMG. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL assay) showed no increase in the number of apoptotic cells in the SMG. CTD, however, induced strongly and transiently (at 1-3 days) the protein expression of LC3B-II, a marker protein of autophagosomes, suggesting that the reduction in the gland weight was due to onset of autophagy by CTD. Upon CTD, Lamp2, a lysosomal marker, gradually increased in amount, reaching a peak at the 14th day. Immunohistochemical analysis revealed an increase in the number of lysosome-like structures positive for both AQP5 and Lamp2 in the acinar cells of the SMG after CTD; similar changes were observed also for AQP5 and LC3Bs. These data suggest that AQP5 in the SMG entered autophagosomes and/or lysosomes for degradation upon CTD. In vitro AQP5-degrading activity was found in the SMG extracts, and such activity was shown to be increased by CTD. Inhibitor experiments implied cathepsins B and L to be candidate enzymes for this degradation under normal and CTD conditions, respectively.
(キーワード): Animals / Apoptosis / Aquaporin 5 / Blotting, Western / Chorda Tympani Nerve / Immunohistochemistry / In Situ Nick-End Labeling / Lysosomal-Associated Membrane Protein 2 / Lysosomes / Male / Parasympathectomy / Proto-Oncogene Proteins c-bcl-2 / Rats / Rats, Sprague-Dawley / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpgi.00194.2010
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20689061; ● Search Scopus @ Elsevier (PMID): 20689061; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpgi.00194.2010

(DOI: 10.1152/ajpgi.00194.2010, PubMed: 20689061) Chenjuan Yao, Nunuk Purwanti, Mileva Ratko Karabasil, Ahmad Azlina, Javkhlan Purevjav, Takahiro Hasegawa, Tetsuya Akamatsu, Toru Hosoi, Koichiro Ozawa and Kazuo Hosoi :
Potential down-regulation of salivary gland AQP5 by LPS via cross-coupling of NF-κB and p-c-Jun/c-Fos,
The American Journal of Pathology, Vol.177, No.2, 724-734, 2010.

(要約): The mRNA and protein levels of aquaporin (AQP)5 in the parotid gland were found to be potentially decreased by lipopolysaccharide (LPS) in vivo in C3H/HeN mice, but only weakly in C3H/HeJ, a TLR4 mutant mouse strain. In the LPS-injected mice, pilocarpine-stimulated saliva production was reduced by more than 50%. In a tissue culture system, the LPS-induced decrease in the AQP5 mRNA level was blocked completely by pyrrolidine dithiocarbamate, MG132, tyrphostin AG126, SP600125, and partially by SB203580, which are inhibitors for IkappaB kinase, 26S proteasome, ERK1/2, JNK, and p38 MAPK, respectively. In contrast, the expression of AQP1 mRNA was down-regulated by LPS and such down-regulation was blocked only by SP600125. The transcription factors NF-kappaB (p65 subunit), p-c-Jun, and c-Fos were increased by LPS given in vivo, whereas the protein-binding activities of the parotid gland extract toward the sequences for NF-kappaB but not AP-1-responsive elements present at the promoter region of the AQP5 gene were increased by LPS injection. Co-immunoprecipitation by using antibody columns suggested the physical association of the three transcription factors. These results suggest that LPS-induced potential down-regulation of expression of AQP5 mRNA in the parotid gland is mediated via a complex(es) of these two classes of transcription factors, NF-kappaB and p-c-Jun/c-Fos.
(キーワード): Animals / Aquaporin 1 / Aquaporin 5 / Cells, Cultured / Enzyme Inhibitors / Humans / JNK Mitogen-Activated Protein Kinases / Lipopolysaccharides / Male / Mice / Mice, Inbred C3H / Mice, Inbred C57BL / NF-kappa B / Nitrates / Nitrites / Proto-Oncogene Proteins c-fos / Salivary Glands
(出版サイトへのリンク): ● Publication site (DOI): 10.2353/ajpath.2010.090282
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20522648; ● Search Scopus @ Elsevier (PMID): 20522648; ● Search Scopus @ Elsevier (DOI): 10.2353/ajpath.2010.090282

(DOI: 10.2353/ajpath.2010.090282, PubMed: 20522648) Mileva Ratko Karabasil, Takahiro Hasegawa, Ahmad Azlina, Nunuk Purwanti, Javkhlan Purevjav, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Trafficking of GFP-AQP5 chimeric proteins conferred with unphosphorylated amino acids at their PKA-target motif (¹⁵²SRRTS) in MDCK-II cells,
The Journal of Medical Investigation : JMI, Vol.56, No.1, 2, 55-63, 2009.

(要約): Three constructs having mutated PKA-target motif at (152)SRRTS of AQP5, an exocrine type water channel, were prepared and fused to C-terminus of green fluorescence protein cDNA to examine the effects of blocking of phosphorylation at (152)SRRTS (a consensus PKA-target motif of AQP5) on translocation or trafficking of the chimeric proteins expressed in the Madin-Darby canine kidney-II (MDCK-II) cells. H-89 treatment increased translocation of wild-type GFP-AQP5 to the apical membrane. All 3 mutant molecules translocated 1.5 to 2 times more than the control wild-type GFP-AQP5. Colchicine but not cytochalasin B inhibited the translocation of wild-type GFP-AQP5. Present results suggest dephosphorylation of this consensus sequence increase GFP-AQP5 translocation, and that microtubules but not microfilaments are involved in this event.
(キーワード): Amino Acid Motifs / Amino Acids / Animals / Aquaporin 5 / Cell Line / Cell Membrane / Chimera / Colchicine / Cyclic AMP-Dependent Protein Kinases / Cytochalasin B / Dogs / Green Fluorescent Proteins / Isoquinolines / Kidney / Phosphorylation / Protein Transport / Sulfonamides
(徳島大学機関リポジトリ): ● Metadata: 111308
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.55
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19262015; ● Search Scopus @ Elsevier (PMID): 19262015; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.55

(徳島大学機関リポジトリ: 111308, DOI: 10.2152/jmi.56.55, PubMed: 19262015) Tetsuya Akamatsu, Ahmad Azlina, Nunuk Purwanti, Mileva Ratko Karabasil, Takahiro Hasegawa, Chenjuan Yao and Kazuo Hosoi :
Inhibition and transcriptional silencing of a subtilisin-like proprotein convertase, PACE4/SPC4, reduces the branching morphogenesis of and AQP5 expression in rat embryonic submandibular gland,
Developmental Biology, Vol.325, No.2, 434-443, 2009.

(要約): The submandibular gland (SMG) develops through the epithelial-mesenchymal interaction mediated by many growth/differentiation factors including activin and BMPs, which are synthesized as inactive precursors and activated by subtilisin-like proprotein convertases (SPC) following cleavage at their R-X-K/R-R site. Here, we found that Dec-RVKR-CMK, a potent inhibitor of SPC, inhibited the branching morphogenesis of the rat embryonic SMG, and caused low expression of a water channel AQP5, in an organ culture system. Dec-RVKR-CMK also decreased the expression of PACE4, a SPC member, but not furin, another SPC member, suggesting the involvement of PACE4 in the SMG development. Heparin, which is known to translocate PACE4 in the extracellular matrix into the medium, and an antibody specific for the catalytic domain of PACE4, both reduced the branching morphogenesis and AQP5 expression in the SMG. The inhibitory effects of Dec-RVKR-CMK were partially rescued by the addition of recombinant BMP2, whose precursor is one of the candidate substrates for PACE4 in vivo. Further, the suppression of PACE4 expression by siRNAs resulted in decreased expression of AQP5 and inhibition of the branching morphogenesis in the present organ culture system. These observations suggest that PACE4 regulates the SMG development via the activation of some growth/differentiation factors.
(キーワード): Amino Acid Chloromethyl Ketones / Animals / Aquaporin 5 / Extracellular Matrix / Furin / Gene Silencing / Heparin / Morphogenesis / Organ Culture Techniques / Proprotein Convertases / Rats / Rats, Sprague-Dawley / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.ydbio.2008.10.015
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 19013448; ● Search Scopus @ Elsevier (PMID): 19013448; ● Search Scopus @ Elsevier (DOI): 10.1016/j.ydbio.2008.10.015

(DOI: 10.1016/j.ydbio.2008.10.015, PubMed: 19013448) Xuefei Li, Ahmad Azlina, Mileva Ratko Karabasil, Nunuk Purwanti, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Degradation of submandibular gland AQP5 by parasympathetic denervation of chorda tympani and its recovery by cevimeline, an M3 muscarinic agonist,
American Journal of Physiology, Gastrointestinal and Liver Physiology, Vol.295, No.1, G112-G123, 2008.

(要約): By chorda tympani denervation (CTD, parasympathectomy), the aquaporin 5 (AQP5), but not AQP1, protein level in the rat submandibular gland (SMG) was significantly decreased, dropping to 37% of that of the contralateral gland at 4 wk. The protein levels of AQP5 and AQP1 were not significantly affected by denervation of the cervical sympathetic trunk (sympathectomy). Administration of cevimeline hydrochloride, an M3 muscarinic receptor agonist (10 mg/kg for 7 days po), but not pilocarpine (0.3 mg/kg for 7 days po), recovered the AQP5 protein level reduced by CTD and increased the AQP1 protein level above the control one. The mRNA level of AQP5 was scarcely affected by CTD and cevimeline hydrochloride administration. Administration of chloroquine (50 mg/kg for 7 days po), a denaturant of lysosomes, increased the AQP5 protein level reduced by CTD. An extract obtained from the submandibular lysosomal fraction degraded the AQP5 protein in the total membrane fraction in vitro. These results suggest the possible regulation of the AQP5 protein level in the SMG by the parasympathetic nerves/M3 muscarinic receptor agonist and imply the involvement of lysosomal enzymes, but not a transcriptional mechanism, in this regulation.
(キーワード): Animals / Aquaporin 1 / Aquaporin 5 / Chorda Tympani Nerve / Gene Expression Regulation / Lysosomes / Male / Muscarinic Agonists / Parasympathectomy / Pilocarpine / Quinuclidines / RNA, Messenger / Rats / Rats, Sprague-Dawley / Submandibular Gland / Sympathectomy / Thiophenes / Time Factors
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpgi.00359.2007
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 18450949; ● Search Scopus @ Elsevier (PMID): 18450949; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpgi.00359.2007

(DOI: 10.1152/ajpgi.00359.2007, PubMed: 18450949) Tetsuya Akamatsu, Nunuk Purwanti, Mileva Ratko Karabasil, Xuefei Li, Chenjuan Yao, Norio Kanamori and Kazuo Hosoi :
Temporospatially regulated expression of subtilisin-like proprotein convertase PACE4 (SPC4) during development of the rat submandibular gland,
Developmental Dynamics, Vol.236, No.1, 314-320, 2007.

(要約): The temporospatial expression of PACE4, a member of the mammalian subtilisin-like proprotein convertase family involved in the activation of growth/differentiation factors, was investigated by in situ hybridization during the development of the rat submandibular gland (SMG). At the initiation stage (day 15.5 of gestation; E15), PACE4 was intensely expressed in the submandibular epithelium, but weakly expressed in the mesenchymal cells. At E16 when the branching morphogenesis becomes obvious, the expression of PACE4 in the mesenchyme was further decreased, although its level in the submandibular epithelium had not changed remarkably from that at E15. During the next stage of embryonic development (E17-E20), PACE4 was expressed in the cells derived from the submandibular epithelium, which include the proacinar, terminal tubular, and presumptive ductal cells. In the perinatal SMG, PACE4 was still expressed intensely in the terminal portion of the SMG containing the proacinar and terminal tubular cells, whereas its expression in the ductal cells was obviously decreased at the second postnatal day (P2) and at P6. Acinar cells expressing no PACE4 appeared, and their numbers increased following their development (P9-P20). At P30 when the PACE4 expression in the acinar cells was completely suppressed, its expression in the ductal cells became intense again. This temporospatially regulated expression of PACE4 suggests its apparent association with the proliferation, differentiation, and establishment of functional acinar and ductal cells of the SMG.
(キーワード): Animals / Female / Gene Expression Regulation, Developmental / Gene Expression Regulation, Enzymologic / In Situ Hybridization / Morphogenesis / Pregnancy / Proprotein Convertases / RNA, Messenger / Rats / Rats, Sprague-Dawley / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1002/dvdy.21008
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 17083113; ● Search Scopus @ Elsevier (PMID): 17083113; ● Search Scopus @ Elsevier (DOI): 10.1002/dvdy.21008

(DOI: 10.1002/dvdy.21008, PubMed: 17083113) Kwartarini Murdiastuti, Nunuk Purwanti, Mileva Ratko Karabasil, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
A naturally occurring point mutation in the rat aquaporin 5 gene, influencing its protein production by and secretion of water from salivary glands,
American Journal of Physiology, Gastrointestinal and Liver Physiology, Vol.291, No.6, GI1081-GI1088, 2006.

(要約): A greater than twofold diversity in the expression level of aquaporin 5 (AQP5) has been observed in the membrane fraction of the submandibular gland (SMG) in Sprague-Dawley rats (Murdiastuti K, Miki O, Yao C, Parvin MN, Kosugi-Tanaka C, Akamatsu T, Kanamori N, and Hosoi K. Pflügers Arch 445: 405-412, 2002). In the present study, breeding between brother and sister rats was repeated within high AQP5 producers and low ones to obtain inbred offspring. High- and low-producer rats from 3rd to 18th generations were used for experiments. By Western blotting, levels of AQP5 proteins in the parotid and lacrimal glands, and lungs were all low in low producers, whereas they were all high in high producers, implying genetic variations of the gene for this water channel. Despite this implication, AQP5 mRNA levels were almost the same between the two groups by Northern blotting, suggesting the irrelevance of transcriptional regulation for this diversity. AQP5 cDNAs from the SMGs of the two groups were sequenced. The nucleotide sequence of AQP5 cDNA from low producers indicated the existence of a point mutation at nt 308 (G308A), leading to a replacement of (103)Gly with (103)Asp in the third transmembrane domain, but no alteration was detected in the Kozak area. The existence of such a mutation was confirmed by the assessment of genomic DNA also. This mutation may have resulted in an abnormal membrane insertion or ineffective trafficking of AQP5, since the rats having this mutation showed extremely low membrane expression of AQP5 in the SMG acinar cells and decreased water secretion from their salivary glands.
(キーワード): Amino Acid Sequence / Animals / Aquaporin 5 / Base Sequence / Body Water / Molecular Sequence Data / Point Mutation / Rats / Salivary Glands / Sequence Homology, Amino Acid / Structure-Activity Relationship
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpgi.00449.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16901987; ● Search Scopus @ Elsevier (PMID): 16901987; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpgi.00449.2005

(DOI: 10.1152/ajpgi.00449.2005, PubMed: 16901987) Chisato Kosugi, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Protein kinase A-regulated membrane trafficking of a green fluorescent protein-aquaporin 5 chimera in MDCK cells,
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol.1763, No.4, 337-344, 2006.

(要約): The green fluorescent protein (GFP) of the jellyfish, Aeqorea victoria, was used as an autofluorescent tag to track the trafficking of aquaporin 5 (AQP5), an exocrine gland-type water channel. Two groups of chimeric proteins were constructed; one in which GFP was fused to the amino-terminus of AQP5 (GFP-AQP5) and the other, in which it was fused to the carboxyl terminus of it (AQP5-GFP). In each group, 2 chimeras were produced, a wild-type AQP5 with its normal sequence and a mutant AQP5 having a mutated amino acid at 259, i.e., GFP-AQP5-T259A and AQP5-GFP-T259A. They were used to transfect Madin-Darby canine kidney (MDCK) cells. The GFP-AQP5 chimera was localized in the intracellular vesicles, which trafficked to the plasma membrane in response to N(6), 2'-O-dibutyryladenosine 3', 5'-cyclic monophosphate (dbcAMP). Membrane trafficking was inhibited by N-[2-(p-bromocinnamylamino)ethyl]-5-isoquimolinesulfonamide (H-89) but not by palmitoyl-dl-carnitine chloride (PCC). In contrast, the AQP5-GFP chimera expressed in MDCK cells was localized constitutively on the plasma membrane. The cellular localization of the latter chimera was not affected by stimulation with dbcAMP in the presence or absence of H-89 or PCC. Replacement of Thr-259 with Ala-259 did not affect the dbcAMP-induced translocation of the chimeric protein, suggesting that phosphorylation of Thr-259 was not necessary for AQP5 trafficking under the present experimental conditions. Thus, the GFP-AQP5 chimera will be a useful tool to study AQP5 trafficking in vitro, whereas the constitutive membrane localization of the AQP5-GFP chimera suggests the importance of the carboxyl terminus of the AQP5 protein for its sorting, whether it is translocated to intracellular vesicles or to the plasma membrane.
(キーワード): Animals / Aquaporin 5 / Cell Line / Cell Membrane / Cyclic AMP-Dependent Protein Kinases / Dogs / Green Fluorescent Proteins / Protein Transport / Recombinant Fusion Proteins
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/j.bbamcr.2006.02.005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16603260; ● Search Scopus @ Elsevier (PMID): 16603260; ● Search Scopus @ Elsevier (DOI): 10.1016/j.bbamcr.2006.02.005

(DOI: 10.1016/j.bbamcr.2006.02.005, PubMed: 16603260) Chenjuan Yao, Mileva Ratko Karabasil, Nunuk Purwanti, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1b in the submandibular gland of mice,
The Journal of Biological Chemistry, Vol.281, No.12, 7968-7976, 2006.

(要約): By using Western blot analysis, high levels of 17.5- and 20-kDa interleukin-1beta (IL-1beta) proteins were detected in the submandibular gland (SMG) of mice. Despite this fact, the amount of pro-IL-1beta protein, a precursor of IL-1beta, with a molecular size of 35 kDa in this tissue was below the detectable level, although strong expression of pro-IL-1beta mRNA was observed. A large amount of 17.5-kDa IL-1beta also appeared in the saliva of mice injected with lipopolysaccharide, suggesting that this IL-1beta is a secretory form produced by the SMG. The protein for IL-1beta-converting enzyme, a processing enzyme for pro-IL-1beta, was expressed only at a low level in the SMG as compared with its level in various epithelial tissues or lipopolysaccharide-stimulated macrophages. On the other hand, mK1, mK9, mK13, and mK22, members of the kallikrein family, were detected strongly in the SMG but not in other tissues. By incubation with mK13, but not with mK1, mK9, or mK22, the 35-kDa pro-IL-1beta was cleaved into two major products with molecular masses of 17.5 and 22 kDa, and production was inhibited by phenylmethylsulfonyl fluoride, a serine protease inhibitor, but not by IL-1beta-converting enzyme inhibitors. A peptide segment corresponding to amino acid residues 107-121 of mouse pro-IL-1beta (107WDDDDNLLVCDVPIR) was cleaved by incubation with mK13, generating two peptides, 107WDDDDNL and 114LVCDVPIR. Therefore, kallikrein mK13 would appear to hydrolyze pro-IL-1beta between its Leu113 and Leu114 residues. The results of immunohistochemistry and an autonomic therapy experiment showed that IL-1beta and kallikrein mK13 were co-localized in the secretory granules of granular convoluted tubular cells. Our present results thus suggest kallikrein mK13 is a plausible candidate for the processing enzyme for pro-IL-1beta in the SMG of mice.
(キーワード): Animals / Blotting, Western / Caspases / Electrophoresis / Enzyme Inhibitors / Enzyme-Linked Immunosorbent Assay / Epithelium / Hydrolysis / Immunoblotting / Immunohistochemistry / Interleukin-1 / Isoenzymes / Kallikreins / Leucine / Lipopolysaccharides / Macrophages / Mice / Mice, Inbred C3H / Models, Genetic / Peptides / Phenylmethylsulfonyl Fluoride / Protease Inhibitors / Protein Binding / RNA, Messenger / Reverse Transcriptase Polymerase Chain Reaction / Saliva / Serine Endopeptidases / Submandibular Gland / Time Factors / Tissue Kallikreins
(出版サイトへのリンク): ● Publication site (DOI): 10.1074/jbc.M507705200
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16423834; ● Search Scopus @ Elsevier (PMID): 16423834; ● Search Scopus @ Elsevier (DOI): 10.1074/jbc.M507705200

(DOI: 10.1074/jbc.M507705200, PubMed: 16423834) Keiko Tsumura, Xuefei Li, Kwartarini Murdiastuti, Most Nahid Parvin, Tetsuya Akamatsu, Chenjuan Yao, Norio Kanamori, Kiyotoshi Inenaga, Hiroshi Yamashita and Kazuo Hosoi :
Downregulation of AQP2 expression in the kidney of polydipsic STR/N mice,
American Journal of Physiology, Renal Physiology, Vol.290, No.2, F478-F485, 2006.

(要約): Aquaporin-2 (AQP2) is responsible for the concentration of urine in the kidney collecting tubule under the regulation of vasopressin. The mRNA level of this water channel in polydipsic STR/N mice was extremely reduced compared with that in normal ICR mice. In male mice, reduction of the AQP2 mRNA level was not evident at 3 wk of age, at which time water intake was not increased. At 10 wk of age, however, the AQP2 mRNA level was reduced to 10% of that in control mice, whereas water intake was increased by 36%. At 44 wk, the water intake became five times that of the control ICR mice, and the AQP2 mRNA level in these polydipsic mice was only approximately 5% of control. Similar changes were observed in the AQP2 protein level, suggesting that the mRNA level of AQP2 reflects the protein level of AQP2. These inverse changes in the AQP2 mRNA level and water intake were also evident in female mice. The data imply that polydipsia in STR/N mice may have affected AQP2 mRNA transcription in the kidney, resulting in reduced AQP2 expression, which would contribute to a reduction in overretention of water.
(キーワード): Age Factors / Animals / Aquaporin 2 / Aquaporins / Body Weight / Diuresis / Down-Regulation / Drinking Behavior / Female / Kidney / Male / Mice / Mice, Inbred ICR / Mice, Inbred Strains / Osmolar Concentration / Polyuria / RNA, Messenger / Reverse Transcriptase Polymerase Chain Reaction / Thirst
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajprenal.00029.2005
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16144968; ● Summary page in Scopus @ Elsevier: 2-s2.0-33644865281

(DOI: 10.1152/ajprenal.00029.2005, PubMed: 16144968, Elsevier: Scopus) Chenjuan Yao, Xuefei Li, Murdiastuti Kwartarini, Chisato Kosugi, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Lipopolysaccharide-induced elevation and secretion of Interleukin-1b in the submandibular gland of male mice,
Immunology, Vol.116, No.2, 213-222, 2005.

(要約): The intraperitoneal injection of lipopolysaccharide (LPS) (400 microg/kg body weight) induced the expression of mRNAs of inflammatory cytokines such as interleukin (IL)-1beta, IL-6 and tumour necrosis factor (TNF)-alpha in the submandibular gland (SMG) of C3H/HeN mice but not that of C3H/HeJ mice, a mutant strain for Toll-like receptor-4 (TLR-4(-) mutant). The mRNA levels of these cytokines in the SMG of the wild-type mice increased as early as 3 hr after injection, peaked at 3-6 hr, and had decreased again by 24 hr. In this study, we particularly focused on IL-1beta, and induction by this endotoxin was investigated in detail. Denervation of the superior cervical trunk and chorda tympani nerve did not diminish the LPS-induced elevation of IL-1beta mRNA in the SMG, indicating the irrelevance of the central nervous system in this induction. TLR-4 mRNA and protein were shown to be strongly expressed in the SMG, suggesting the direct action of LPS on this gland. IL-1beta proteins were localized in the secretory granules of granular convoluted tubular (GCT) cells, and their molecular weights in the gland were 17.5 and 20 kDa. IL-1beta of the same size appeared in the saliva 6 hr after LPS injection in C3H/HeN but not in C3H/HeJ mice. The present study thus suggests that IL-1beta, an inflammation cytokine, is induced and secreted into the saliva in response to endotoxin injected intraperitoneally.
(キーワード): Animals / Blotting, Western / Cytokines / Gene Expression Regulation / Injections, Intraperitoneal / Interleukin-1 / Lipopolysaccharides / Male / Mice / Mice, Inbred C3H / RNA, Messenger / Reverse Transcriptase Polymerase Chain Reaction / Saliva / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1111/j.1365-2567.2005.02212.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 16162270; ● Search Scopus @ Elsevier (PMID): 16162270; ● Search Scopus @ Elsevier (DOI): 10.1111/j.1365-2567.2005.02212.x

(DOI: 10.1111/j.1365-2567.2005.02212.x, PubMed: 16162270) Most Nahid Parvin, Shingo Kurabuchi, Kwartarini Murdiastuti, Chenjuan Yao, Chisato Kosugi, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Subcellular redistribution of AQP5 by vasoactive intestinal polypeptide (VIP) in the Brunner's gland of the rat duodenum,
American Journal of Physiology, Gastrointestinal and Liver Physiology, Vol.288, No.6, G1283-G1291, 2005.

(要約): Aquaporin (AQP)5, an exocrine-type water channel, was detected in the rat duodenum by Western blot analysis, and was localized by immunohistochemistry in the secretory granule membranes as well as in the apical and lateral aspects of the plasma membrane of Brunner's gland cells. Incubation of duodenal slices with vasoactive intestinal polypeptide (VIP) in vitro significantly increased the amount of AQP5 in the apical membrane fraction in a dose- and time-dependent manner with the amount reaching a plateau at 100 nM VIP and becoming near maximal after a 30-s incubation. Protein kinase inhibitors, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7, 50 muM), and N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89; PKA-specific, 1 muM) blocked this increase, but PKC-specific inhibitor calphostin C did not, implying the involvement of PKA but not PKC in this cellular event. Intravenous injection with VIP (40 mug/kg body wt) provoked dilation of the lumen of the Brunner's gland at 2 and 7 min and increased the staining intensity of AQP5 in the apical and lateral membranes. AQP1 (both nonglycosylated and glycosylated forms) was also found to localize in the apical and basolateral membranes of cells of Brunner's gland. VIP, however, did not provoke any significant change in the AQP1 level in the apical membrane, as judged from the results of the above in vitro and in vivo experiments. These results suggest that VIP induced the exocytosis of granule contents and simultaneously caused translocation of AQP5 but not of AQP1 to the apical membrane in Brunner's gland cells.
(キーワード): Animals / Aquaporin 1 / Aquaporin 5 / Aquaporins / Brunner Glands / Dose-Response Relationship, Drug / Exocytosis / Intracellular Signaling Peptides and Proteins / Male / Membrane Proteins / Protein Kinases / Rats / Rats, Sprague-Dawley / Vasoactive Intestinal Peptide
(出版サイトへのリンク): ● Publication site (DOI): 10.1152/ajpgi.00030.2004
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 15650134; ● Search Scopus @ Elsevier (PMID): 15650134; ● Search Scopus @ Elsevier (DOI): 10.1152/ajpgi.00030.2004

(DOI: 10.1152/ajpgi.00030.2004, PubMed: 15650134) Tetsuya Akamatsu, Most Nahid Parvin, Kwartarini Murdiastuti, Chisato Kosugi, Chenjuan Yao, Osamu Miki, Norio Kanamori and Kazuo Hosoi :
Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland.,
Pflügers Archiv : European Journal of Physiology, Vol.446, No.6, 641-651, 2003.

(要約): ラット顎下腺発生過程でのAQPファミリーの発現を解析した．AQP3,4は胎生期に非常に弱く発現するが，AQP1,5は胎生期から生後を通して発現し続ける．AQP1は血管にのみ局在するのに対して，AQP5は胎生18日の未分化腺房細胞管腔膜への局在以後，分化成熟につれ腺房細胞の他，一部の血管にも局在する．発生過程を通しAQP5が唾液の産生·分泌に中心的役割を果たすと考えられる．
(キーワード): Aging / Amino Acid Sequence / Animals / Aquaporins / Biological Transport, Active / Blotting, Northern / Body Water / Female / Immunohistochemistry / Molecular Sequence Data / Pregnancy / RNA / RNA, Antisense / Rats / Rats, Sprague-Dawley / Reverse Transcriptase Polymerase Chain Reaction / Sodium-Potassium-Exchanging ATPase / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00424-003-1109-9
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 12838423; ● Search Scopus @ Elsevier (PMID): 12838423; ● Search Scopus @ Elsevier (DOI): 10.1007/s00424-003-1109-9

(DOI: 10.1007/s00424-003-1109-9, PubMed: 12838423) Kwartarini Murdiastuti, Osamu Miki, Chenjuan Yao, Most Nahid Parvin, Chisato Kosugi, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Divergent expression and localization of aquaporin 5, an exocrine-type water channel, in the submandibular gland of Sprague-Dawley rats.,
Pflügers Archiv : European Journal of Physiology, Vol.445, No.3, 405-412, 2002.

(要約): 正常SDラット唾液腺(顎下腺)のAQP5蛋白質発現レベルを調べた所，高発現ラットおよび低発現ラットが存在する事を見いだした．2種のラットを近交化する目的で兄妹交配を進め，現在高発現ラットは8代目に，また低発現ラットは4代目に到った．これらのラットでは発現レベルの差は100倍以上に達した．AQP5蛋白質発現レベルの差は耳下腺，肺，涙腺においても認められたが，AQP5 mRNAレベルはいずれの臓器においても両グループ間で差はなかった．したがって，上記2グループ間で認められたAQP5蛋白質発現レベルの差異はAQP5遺伝子あるいはそのプロモーターに発生した遺伝的変異に基づくものと考えられた．
(キーワード): aquaporin 5 / genetic variation / Sprague-Dawley (SD) rat / submandibular gland / KIDNEY COLLECTING DUCT / MOLECULAR-CLONING / FUNCTIONAL EXPRESSION / BASOLATERAL MEMBRANE / EPITHELIAL-CELLS / LACRIMAL GLANDS / PROTEIN / IMMUNOLOCALIZATION / GLYCEROL / UREA
(出版サイトへのリンク): ● Publication site (DOI): 10.1007/s00424-002-0940-8
(文献検索サイトへのリンク): ● Search Scopus @ Elsevier (DOI): 10.1007/s00424-002-0940-8

(DOI: 10.1007/s00424-002-0940-8) Most Nahid Parvin, Keiko Tsumura, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Expression and localization of AOP5 in the stomach and duodenum of the rat.,
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol.1542, No.1-3, 116-124, 2002.

(要約): 消化管におけるアクアポリン5(AQP5)の発現を，RT-PCRおよびウェッスタンブロッティングによってしらべ，下部胃および十二指腸にその強い発現を認めた．検出したAQP5蛋白質は従来の報告のとおり27kDaであった．発現部位は胃では幽門腺，十二指腸ではブルーナー腺であった．これらの結果はAQP5は胃および十二指腸における水分泌に関与する可能性を示唆する．
(キーワード): Animals / Aquaporin 5 / Aquaporins / Biological Transport / Duodenum / Fluorescein-5-isothiocyanate / Gastric Mucosa / Immunohistochemistry / Male / Membrane Proteins / RNA, Messenger / Rats / Rats, Sprague-Dawley / Reverse Transcriptase Polymerase Chain Reaction / Stomach / Water
(出版サイトへのリンク): ● Publication site (DOI): 10.1016/S0167-4889(01)00172-0
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11853885; ● Search Scopus @ Elsevier (PMID): 11853885; ● Search Scopus @ Elsevier (DOI): 10.1016/S0167-4889(01)00172-0

(DOI: 10.1016/S0167-4889(01)00172-0, PubMed: 11853885) Norio Kanamori, Tetsuya Akamatsu, Chisato Kosugi, Most Nahid Parvin and Kazuo Hosoi :
Methylene blue as a vascular filling dye.,
ITE Leters on Batteries, New Technologies & Medicine (with News), Vol.3, No.1, 75-77, 2002.

(要約): メチレンブルー溶液とピクリン酸溶液とを混合すると，色素の濃度に依存して，ゲル状∼ゴム状の沈澱を生ずることを見出した．この沈殿物を回収してその吸収パターンを計測した結果，メチレンブルーそのものであり，ピクリン酸との化合物や共沈殿物ではないことが判明した．すなわち，ピクリン酸による塩析効果によって色素が沈澱する．ピクリン酸を含むブアン液によって潅流固定した実験動物の血管内にメチレンブルー溶液を注入することにより，生成したゴム状またはゲル状の茶色の色素によって血管内を満たすことができる．通常の色素による染色では，すべての血管が染色され，組織は瀰慢性に濃染してしまう．この新たに開発した方法は，色素濃度を加減することによって生成物の粘性を選ぶことができ，色素進入の血管径を選択できるので，太い血管から順に色素を充填することが可能で，血管網のin situ観察に高い利用価値を有する．

Wei Wei, Most Nahid Parvin, Keiko Tsumura, Tetsuya Akamatsu, Jun Tada, Norio Kanamori and Kazuo Hosoi :
Induction of C-reactive protein, serum amyloid P component, and kininogens in the submandibular and lacrimal glands of rats with experimentally induced inflammation.,
Life Sciences, Vol.69, No.3, 359-368, 2001.

(要約): テルペンチン油を投与した実験的炎症モデルラットにおいて，投与24時間後に，C-反応生蛋白質，血清アミロイド蛋白質，H-およびT-キニノーゲンのmRNAが，顎下腺，眼窩内および眼窩外涙腺に誘導されることを見いだした．投与6時間後に，この誘導に先立って，TNF-αmRNAが誘導された．顎下腺，眼窩内および眼窩外涙腺におけるこれら急性期蛋白質の誘導は，外分泌腺に強い防御系が存在することを示唆するものと考えられる．

Most Nahid Parvin, Kwartarini Murdiastuti, Tetsuya Akamatsu, Norio Kanamori, Norihiko Maeda and Kazuo Hosoi :
Expression and locarization of AQP4 in the crypt epithelial cells of the rat duodenum.,
Biomedical Research (India), Vol.12, No.2, 77-82, 2001.

(要約): RT-PCRによってアクアポリン4(AQP4)の消化管における発現を調べた．AQP4は下部胃，十二指腸，空腸，回腸，盲腸，大腸，および直腸に発現していたが上部胃ニは発現を認めなかった．ウェッスタンブロッティングで十二指腸に30kDaのAQP4蛋白質の発現を認めた．滅め貴組織化学実験によりAQP4は十二指腸上皮の陰窩細胞の基底側膜に局在したが絨毛細胞には局在しなかった．

Norio Kanamori, Tetsuya Akamatsu, Jun Tada, Keiko Tsumura, Most Nahid Parvin, Kwartarini Murdiastui, Wei Wei and Kazuo Hosoi :
Search for the dye that stains the vascular system.,
ITE Leters on Batteries, New Technologies & Medicine (with News), Vol.2, No.1, 116-119, 2001.

(要約): 血管系の，特に動脈系に特異的なin situ染色法を開発する目的で，数種の染料を選び，その染色性その他の諸性質を検討した．いくつかの候補の中から，ルクソールファストブルーが最も望ましい性質を有することを明らかにした．すなわち，同色素溶液はピクリン酸溶液と接触すると複塩または錯塩を生成し，原色素とは異なる吸収曲線を呈する沈澱を生じる．ピクリン酸を含む固定液で潅流したのち左心室より同色素液を注入すると，動脈系だけが染色される．このようなin situ血管染色法は，今までになかったものである．

Tetsuya Akamatsu, Yoshiko Matsuda, Keiko Tsumura, Jun Tada, Most Nahid Parvin, Wei Wei, Norio Kanamori and Kazuo Hosoi :
Highly regulated expression of subtilisin-like proprotein convertase PACE4 (SPC4) during dentinogenesis.,
Biochemical and Biophysical Research Communications, Vol.272, No.2, 410-415, 2000.

(要約): サチライシン様プロセシング酵素PACE4は遺伝子プロモーター領域の構造から，その発現は極めて複雑に制御されると考えられたが，生体内での発現制御については不明であった．本研究により，歯牙形成過程において実際に発現が複雑に制御されていること，また，この様な発現制御がファミリーの中でもPACE4に特有であることが初めて明らかになった．
(キーワード): Processing protease / PACE4 / Dentinogenesis / Gene expression
(出版サイトへのリンク): ● Publication site (DOI): 10.1006/bbrc.2000.2752
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 10833428; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034616641

(DOI: 10.1006/bbrc.2000.2752, PubMed: 10833428, Elsevier: Scopus) Kazuo Hosoi, Sachiko Matsuura, Keiko Tsumura, Wei Wei, Most Nahid Parvin, Jun Tada, Tetsuya Akamatsu, Norio Kanamori and Kazuo Suzuki :
Expression of kininogens in the connective tissue-type mast cells of the rats.,
Immunology, Vol.101, No.4, 531-540, 2000.

(要約): ラット顎下腺および腹腔に存在する結合組織型肥満細胞にキニノーゲン(KG)が発現していることをRT-PCR，ウェッスタンブロッティング，免疫組織化学的手法を用いて明らかにした．発現しているKGは高分子型およびT-1KGであった．KGは肥満細胞分泌顆粒に局在した．RT-PCRサザンブロッティングにより分離腹腔肥満細胞にKG mRNAを検出したのでKGは肥満細胞でde novoの合成が行われて入ることが示唆された．
(キーワード): Animals / Ascitic Fluid / Blotting, Western / Gene Expression / Immunoenzyme Techniques / Kininogens / Male / Mast Cells / Molecular Weight / RNA, Messenger / Rats / Rats, Wistar / Reverse Transcriptase Polymerase Chain Reaction / Submandibular Gland
(出版サイトへのリンク): ● Publication site (DOI): 10.1046/j.1365-2567.2000.00132.x
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 11122457; ● Summary page in Scopus @ Elsevier: 2-s2.0-0034537325

(DOI: 10.1046/j.1365-2567.2000.00132.x, PubMed: 11122457, Elsevier: Scopus) Jun Tada, Takamasa Sawa, Naoki Yamanaka, Masayuki Shono, Tetsuya Akamatsu, Keiko Tsumura, Most Nahid Parvin, Norio Kanamori and Kazuo Hosoi :
Involvement of vesicle-cytoskeleton interaction in AQP5 trafficking in AQP5-gene-transfected HSG cells.,
Biochemical and Biophysical Research Communications, Vol.266, No.2, 443-447, 1999.

(要約): ヒト唾液腺細胞にアクアポリン5(AQP5)を遺伝子導入したところ，AQP5蛋白質は細胞内の小胞に発現した．タプシガージン等により細胞内カルシウムを上昇させると小胞のAQP5蛋白質は細胞膜へ移動した．さらに微小管阻害剤である，コルヒチンおよびビンブラスチンはこの移動を抑制した．AQP5のトラッフィッキングは微小管が関与して入る可能性が示唆された．
(キーワード): Aquaporin 5 / Water channel / Trafficking / Cytoskeleton

Tetsuya Akamatsu, Yoshiko Matsuda, Keiko Tsumura, Jun Tada, Most Nahid Parvin, Norio Kanamori and Kazuo Hosoi :
Subtilisin-like proprotein convertase PACE4 (SPC4) is a candidate processing enzyme of bone morphogenetic proteins during tooth formation,
Developmental Dynamics, Vol.216, No.4/5, 481-488, 1999.

(要約): サチライシン様プロセシング酵素PACE4が歯牙形成過程において時期·空間特異的に発現し，歯牙形成に必須の骨形成蛋白質群(BMPs)と一致して局在することから， PACE4が歯牙形成時の最も有力なBMPsプロセシング酵素で， BMPsの活性化によりエナメル芽細胞と象牙芽細胞の分化成熟と密接に関わることを見い出した．
(キーワード): Processing protease / PACE4 / Bone morphogenetic protein / Tooth / Development

Akihiko Tsuji, Chiemi Hine, Yasuhiro Tamai, Kayoko Yonemoto, Kazushige Mori, Shigeru Yoshida, Miwa Bando, Eri Sakai, Kenji Mori, Tetsuya Akamatsu and Yoshiko Matsuda :
Genomic organization and alternative splicing of human PACE4 (SPC4), Kexin-like processing endoprotease.,
The Journal of Biochemistry, Vol.122, No.2, 438-452, 1997.

(要約): 本研究ではプロセシング酵素Kexin familyの1つPACE4のヒトゲノムを単離し, その構造を解析し, ファミリーの中でも最も大きく複雑な構造であること, 多種類存在するアイソフォームが全て同一遺伝子から選択的スプライシングにより生じること, およびプロモーター領域がユニークな構造であること等を初めて明らかにした.
(キーワード): Processing protease / PACE4 / Gene structure / Alternative splicing

Tetsuya Akamatsu, Shigeo Daikoku, Hideaki Nagamune, Shigeru Yoshida, Kenji Mori, Akihiko Tsuji and Yoshiko Matsuda :
Developmental expression of a novel Kexin family protease, PACE4E, in the rat olfactory system.,
Histochemistry and Cell Biology, Vol.108, No.2, 95-103, 1997.

(要約): 本研究では新規Kexin family proteaseであるPACE4Eがラット脳発生過程において時期·空間特異的に発現することを明らかにし, 特に嗅球においては僧帽細胞に極めて特異的に発現し, 僧帽細胞の分化成熟と密接に関わることを見い出した.
(キーワード): Processing protease / Olfactory system / Mitral cell

Kenji Mori, Sachiko Kii, Akihiko Tsuji, Masami Nagahama, Akiyoshi Imamaki, Keiko Hayashi, Tetsuya Akamatsu, Hideaki Nagamune and Yoshiko Matsuda :
A Novel Human PACE4 Isoform, PACE4E Is an Active Processing Protease Containing a Hydrophobic Cluster at the Carboxy Terminus,
The Journal of Biochemistry, Vol.121, No.5, 941-948, 1997.

(要約): ズブチリシン様プロプロテインコンベルターゼファミリーに属するPACE4の新規アイソフォームPACE4E-I, PACE4E-IIをヒト脳cDNAライブラリーよりクローニングした．PACE4Eは，PACE4Aと比べ，C末に75残基からなる，疎水性アミノ酸のクラスタを含むユニークな配列を持っていた．この部分を欠失したPACE4Eは分泌速度が上昇した．共発現実験によって，PACE4E-1, E-IIは，PACE4Aと同様にプロセシング活性を持っていることが証明された．

Hideaki Nagamune, Kazuaki Muramatsu, Tetsuya Akamatsu, Yasuhiro Tamai, Keisuke Izumi, Akihiko Tsuji and Yoshiko Matsuda :
Distribution of the kexin family proteases in pancreatic islets., --- PACE4C is specifically expressed in B cells of pancreatic islets ---,
Endocrinology, Vol.136, No.1, 357-360, 1995.

(要約): Kexin Family Proteasesはペプチドホルモン等のプロセシング酵素として同定され, 血糖調節ホルモンのインスリン等のプロセシングにも関与することが示唆されていたが, 本研究では我々が新規にクローニングしたPACE4CをはじめとするKexin Family Proteasesとインスリン等との膵島細胞での局在, 共存関係を初めて明らかにし, その成果は雑誌表紙にも掲載された.
(キーワード): Precessing protease / Pancreatic islet / Insulin / Glucagon

Akihiko Tsuji, Tetsuya Akamatsu, Hideaki Nagamune and Yoshiko Matsuda :
Identification of targeting proteinase for rat α1-macroglobulin in vivo., --- Mast-cell tryptase is a major component of the α1-macroglobulin-proteinase complex endocytosed into rat liver lysosomes. ---,
The Biochemical Journal, Vol.298, No.1, 79-85, 1994.

(要約): 血清蛋白質であるα-マクログロブリン(αM)は種々の酵素に対するインヒビターとして機能すると考えられていたが, 生体内で標的となる酵素は不明であった. 本研究ではαMが酵素と複合体形成後, リソゾームに運ばれ分解されることに着目し, リソゾーム内の分解を抑制したラット肝臓リソゾームよりαM-酵素複合体を単離し, 肥満細胞トリプターゼが生体内でαMの標的酵素の1つであることを初めて明らかにした.
(キーワード): α-macroglobulin / Mast-cell tryptase / Lysosome

MISC

Chenjuan Yao, Tetsuya Akamatsu, Takahiro Hasegawa and Hiroshi Yoshimura :
The Defense System of Oral Cavity: Lipopolysaccharide Induced Inflammatory Response in the Mice Salivary Gland,
BIT's 1st Annual World Congress of Oral & Dental Medicine Conference Abstract Book, 107, 2014.

(キーワード): 唾液腺 (salivary gland) / 炎症 (inflammation) / 炎症性サイトカイン (inflammatory cytokine) / リポ多糖

Tetsuya Akamatsu, Chenjuan Yao, Takahiro Hasegawa and Hiroshi Yoshimura :
Salivary Gland Development, Differentiation, and Regeneration - Role of Subtilisin-like Proprotein Convertase PACE4/SPC4,
BIT's 1st Annual World Congress of Oral & Dental Medicine Conference Abstract Book, 59, 2014.

(キーワード): 唾液腺 (salivary gland) / 発生 (development) / 分化 (differentiation) / 再生 (regeneration) / 前駆体蛋白質変換酵素

長谷川敬展, 姚陳娟, 赤松徹也, 吉村弘 :
ユビキチンリガーゼによるAQP5のユビキチン化亢進とダウンレギュレーション,
Journal of Oral Biosciences, Vol.Suppl., 204, 2014年.

(キーワード): 水チャネル (water channel) / ユビキチン化 / ダウンレギュレーション / AQP5

姚陳娟, 赤松徹也, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおける水チャネルAQP5の発現,
Journal of Oral Biosciences, Vol.Suppl., 204, 2014年.

(キーワード): 唾液腺 (salivary gland) / 再生 (regeneration) / 水チャネル (water channel) / AQP5

嶺岸誠, 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおけるサチライシン様前駆体蛋白質変換酵素の発現誘導,
Journal of Oral Biosciences, Vol.Suppl., 138, 2014年.

(キーワード): 唾液腺 (salivary gland) / 再生 (regeneration) / 前駆体蛋白質変換酵素

総説・解説

Kazuo Hosoi, Chenjuan Yao, Takahiro Hasegawa, Hiroshi Yoshimura and Tetsuya Akamatsu :
Dynamics of Salivary Gland AQP5 under Normal and Pathologic Conditions,
International Journal of Molecular Sciences, Vol.21, No.4, 1182, Feb. 2020.

(徳島大学機関リポジトリ): ● Metadata: 114575
(出版サイトへのリンク): ● Publication site (DOI): 10.3390/ijms21041182
(文献検索サイトへのリンク): ● Summary page in Scopus @ Elsevier: 2-s2.0-85079334066

(徳島大学機関リポジトリ: 114575, DOI: 10.3390/ijms21041182, Elsevier: Scopus) 赤松徹也 :
唾液腺の構造と機能,
Journal of Oral Health and Biosciences, Vol.28, No.2, 77-86, 2016年2月.

(要約): The salivary gland is developed under the epithelial-mesenchymal interaction and formed by repeating branching morphogenesis, which is a common process in the development of the glandular tissues. Although the differentiation and maturation of salivary gland is continued into early postnatal life, the fundamental ability of saliva secretion is already expressed after birth.These development, differentiation, and maturation of salivary gland are regulated by many growth/ differentiation factors, which are initially synthesized as inactive precursors and activated by the limited proteolysis. On the other hand, saliva secretion is one of the important physiological functions of the salivary gland and occurs dependently on the increase of the osmolality in the lumen through two pathways, paracellular and transcellular pathways. It is revealed that a water channel aquaporin 5 (AQP5) is involved in saliva secretion through the latter pathway. Saliva contains various components, which express various physiological functions of saliva. Because the oral cavity is confronted with hazards of various allergens from the outside continuously, the salivary gland produces and secretes various factors as the defense system. Saliva is, therefore, one of an important body fluid to maintain the oral health, and the decrease of saliva secretion causes xerostomia/dry mouth, which affects not only oral disease and dysfunction but also systemic disease. We previously reported the involvement of a subtilisin-like proprotein convertase PACE4 in the development and differentiation of salivary gland, lipopolysaccharide-mediated induction of inflammatory cytokines in the salivary gland, degradation of salivary AQP5 by parasympathetic denervation, and so on. This review will describe the structure and function of the salivary gland, from its development to functional expression and regulation.
(キーワード): 唾液腺 (salivary gland) / 唾液 / 水チャネル / サチライシン様前駆体蛋白質変換酵素
(徳島大学機関リポジトリ): ● Metadata: 109872
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1050564287418446592

(徳島大学機関リポジトリ: 109872, CiNii: 1050564287418446592) Tetsuya Akamatsu :
Physiological role of a subtilisin-like proprotein convertase, PACE4, in submandibular gland development,
Journal of Oral Biosciences, Vol.52, No.2, 81-93, May 2010.

(キーワード): subtilisin-like proprotein convertase / PACE4 / submandibular gland / branching morphogenesis / AQP5
(出版サイトへのリンク): ● Publication site (DOI): 10.2330/joralbiosci.52.81
(文献検索サイトへのリンク): ● CiNii @ 国立情報学研究所 (CRID): 1390001205213224960; ● Search Scopus @ Elsevier (DOI): 10.2330/joralbiosci.52.81

(DOI: 10.2330/joralbiosci.52.81, CiNii: 1390001205213224960) Most Parvin Nahid, Jun Tada, Tetsuya Akamatsu and Kazuo Hosoi :
Structure, function, and transcriptional regulation of aquaporins, proteins of the water channel family,
Dentistry in Japan, Vol.37, 26-31, Mar. 2001. Yoshiko Matsuda, Akihiko Tsuji, Hideaki Nagamune, Tetsuya Akamatsu, Chiemi Hine, Kazuaki Muramatsu, Kenji Mori, Yasuhiro Tamai and Kayoko Yonemoto :
Novel members of mammalian Kexin family proteases, PACE4C, PACE4D, PC7A and PC7B.,
Advances in Experimental Medicine and Biology, Vol.389, 63-71, Sep. 1996.

(要約): ペプチドホルモンや増殖因子等は不活性な前駆体として合成され, 特定の塩基性アミノ酸部位での限定切断を経て初めて活性化される. Kexin family proteaseはこのプロセシングを担う重要な酵素であり, 本研究では我々が新規メンバーをクローニングし, その構造, 組織分布等を明らかにした.
(キーワード): Processing protease

講演・発表

Chenjuan Yao, Tetsuya Akamatsu, Takahiro Hasegawa and Hiroshi Yoshimura :
Induced expression of a subtilisin-like proprotein convertase PACE4 in the regeneration model of rat submandibular gland.,
The 4th International Symposium on Salivary Glands in Honor of Niels Stensen, Okazaki (Japan), Nov. 2016. Tetsuya Akamatsu, Chenjuan Yao, Takahiro Hasegawa and Hiroshi Yoshimura :
Sexual difference in the regeneration model of the rat submandibular gland.,
The 4th International Symposium on Salivary Glands in Honor of Niels Stensen, Okazaki (Japan), Nov. 2016. Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Hiroshi Yoshimura :
Post-translational modifications of water channel aquaporin-5 in salivary gland cells, Oral Neuroscience 2016,
Oral Neuroscience 2016, Osaka, Oct. 2016. Hiroshi Yoshimura, Tetsuya Akamatsu, Chenjuan Yao and Takahiro Hasegawa :
Synaptic plasticity in the brain -Roles of NMDA receptor- (Invited lecture at Nantong University),
Sep. 2016.

(キーワード): synaptic plasticity / NMDA受容体 (NMDA-receptors)

Chenjuan Yao, Tetsuya Akamatsu, Takahiro Hasegawa and Hiroshi Yoshimura :
The Defense System of Oral Cavity: Lipopolysaccharide Induced Inflammatory Response in the Mice Salivary Gland,
BIT's 1st Annual International Congress of Oral & Dental Medicine, Session 9: Oral Immunological Diseases, Allergy and Inflammation, Haikou, China, Nov. 2014.

(キーワード): 唾液腺 (salivary gland) / 炎症 (inflammation) / 炎症性サイトカイン (inflammatory cytokine) / リポ多糖

Tetsuya Akamatsu, Chenjuan Yao, Takahiro Hasegawa and Hiroshi Yoshimura :
Salivary Gland Development, Differentiation, and Regeneration - Role of Subtilisin-like Proprotein Convertase PACE4/SPC4,
BIT's 1st Annual International Congress of Oral & Dental Medicine, Session 3: Oral Biology, Haikou, China, Nov. 2014.

(キーワード): 唾液腺 (salivary gland) / 発生 (development) / 分化 (differentiation) / 再生 (regeneration) / 前駆体蛋白質変換酵素

Mileva Ratko Karabasil, Kwartarini Murdiastuti, Nunuk Purwanti, Azlina Ahmad, Javkhlan Purevjav, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Effects of natural point mutation of rat aquaporin 5 expressed in vitro on its capacity of water permeability and membrane trafficking,
The Journal of Medical Investigation : JMI, Vol.56, No.Suppl, 398-400, Tokushima, Dec. 2009.

(要約): In the colony of Sprague-Dawley (SD) strain, we found that there were rats expressing a mutant AQP5, which has a point mutation at nt 308 (G308A), leading to a replacement of (103)Gly with (103)Asp in the 3rd transmembrane domain. The mutant molecule scarcely expressed in the acinar cells, probably because of ineffective trafficking. The mutant molecule, however, showed normal water permeability when assessed by the oocyte system.
(キーワード): Amino Acid Sequence / Animals / Aquaporin 5 / Biological Transport / Cell Membrane Permeability / Female / Models, Animal / Molecular Sequence Data / Oocytes / Point Mutation / Rats / Rats, Sprague-Dawley / Submandibular Gland / 水チャネル (water channel) / Xenopus laevis
(徳島大学機関リポジトリ): ● Metadata: 111488
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.398
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20224238; ● Search Scopus @ Elsevier (PMID): 20224238; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.398

(徳島大学機関リポジトリ: 111488, DOI: 10.2152/jmi.56.398, PubMed: 20224238) Purevjav Javkhlan, Yuka Hiroshima, Azlina Ahmad, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Jun-ichi Kido, Toshihiko Nagata and Kazuo Hosoi :
Induction of calprotectin mRNAs by lipopolysaccharide in the salivary gland of mice,
The Journal of Medical Investigation : JMI, Vol.56, No.Suppl, 287-289, Tokushima, Dec. 2009.

(要約): Calprotectin is a major cytosolic calcium-binding protein of leukocytes which belongs to the S100 protein family. S100A8 and S100A9, major types of calprotectin are heterodimeric complexes being composed of light- and heavy-chain subunits. The calprotectin levels in the plasma, feces, synovial fluid, gingival crevicular fluid, dental calculus and saliva change when the host animal suffers from several inflammatory diseases. Members of Toll-like receptor (TLR) family are pattern-recognition receptors for lipopolysaccharide (LPS) and other pathogens. Here we examined if the biological role of TLR receptor is reflected to the calprotectin expression in the salivary gland. Time course study by using real-time RT-PCR detected higher levels of S100A8 and S100A9 mRNA at 1.5-3 h after injection of LPS in both the submandibular gland (SMG) and parotid gland (PG) of C3H/HeN mice but not in the same tissues of C3H/HeJ, a TLR-4 mutant strain, indicating that this induction is mediated via the TLR-4. These results indicate that, an inflammatory marker, calprotectin, is expressed in the mouse salivary gland and that LPS stimulated its synthesis. Calprotectin (S100A8/A9) showed minimum expression in all cellular segments in the SMG except excretory duct cells, which showed strong signal at the cytoplasm. LPS induced their expressions in the granular convoluted tubular cells and striated duct cells. In the PG, these proteins were expressed very weakly in both duct and acinar cells with a little stronger staining for the former cells. LPS injection induced calprotectin (S100A8/A9) in both duct and acinar cells especially in the former cells.
(キーワード): Animals / Calgranulin A / Calgranulin B / Leukocyte L1 Antigen Complex / Lipopolysaccharides / Mice / Mice, Inbred C3H / Mice, Mutant Strains / Parotid Gland / RNA, Messenger / 唾液腺 (salivary glands) / Submandibular Gland / Toll-Like Receptor 4
(徳島大学機関リポジトリ): ● Metadata: 111354
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.287
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20224205; ● Search Scopus @ Elsevier (PMID): 20224205; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.287

(徳島大学機関リポジトリ: 111354, DOI: 10.2152/jmi.56.287, PubMed: 20224205) Azlina Ahmad, Xuefei Li, Purevjav Javkhlan, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Down-regulation of submandibular gland AQP5 following parasympathetic denervation in rats,
The Journal of Medical Investigation : JMI, Vol.56, No.Suppl, 273-276, Tokushima, Dec. 2009.

(要約): Following chorda tympani denervation (CTD, parasympathetomy), the protein levels of aquaporin5 (AQP5) as well as AQP1 and Na(+)K(+)ATPase alpha-subunit in the rat submandibular gland (SMG) were found to be decreased significantly. However, the level of another membrane protein, dipeptidyl peptidase IV was not affected by CTD, suggesting a selective reduction of AQP5, AQP1, and Na(+)K(+)ATPase alpha -subunit proteins by CTD. However, the AQP5 mRNA level was scarcely affected by CTD, which suggested that transcription process of AQP5 was unaffected by this operation. AQP5 protein was shown to be degraded in vitro by the extract of the SMG obtained from normal rat; inhibitor experiments in vitro suggested cathepsin B was a responsible enzyme. Co-localization of AQP5 and LAMP-2, a lysosomal marker, implicated AQP5 is degraded in lysosomes. A significant increase in the protein levels of LC3-II, an autophagy marker, at day 1 after CTD, and co-localization of the LC3 protein and AQP5, suggested that CTD activated autophagy of SMG, leading to AQP5 degradation.
(キーワード): Animals / Aquaporin 1 / Aquaporin 5 / オートファジー (autophagy) / Chorda Tympani Nerve / Down-Regulation / Lysosomal-Associated Membrane Protein 2 / Male / Microtubule-Associated Proteins / Parasympathectomy / RNA, Messenger / Rats / Rats, Sprague-Dawley / Sodium-Potassium-Exchanging ATPase / Submandibular Gland
(徳島大学機関リポジトリ): ● Metadata: 111348
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.273
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20224200; ● Search Scopus @ Elsevier (PMID): 20224200; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.273

(徳島大学機関リポジトリ: 111348, DOI: 10.2152/jmi.56.273, PubMed: 20224200) Nunuk Purwanti, Azlina Ahmad, Mileva Ratko Karabasil, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Involvement of the IL-6/STAT3/Sca-1 system in proliferation of duct cells following duct ligation in the submandibular gland of mice,
The Journal of Medical Investigation : JMI, Vol.56, No.Suppl, 253-254, Tokushima, Dec. 2009.

(要約): Ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) induced the expression of Sca-1, a stem cell marker. Sca-1 expression increased prominently in almost all of cells in the duct system, except the acinar cells. Sca-1 induction was accompanied with phosphorylated-STAT3 (Y705) elevation, which was localized in the nuclei of all duct cells. Electrophoretic mobility shift assay (EMSA) confirmed the specific binding of STAT3 to the GAS sequence, a biding site of gamma interferon activating site. Present study suggested one of the initial steps of the tissue regeneration after injury includes STAT3 pathway.
(キーワード): Animals / Antigens, Ly / Cell Proliferation / 炎症 (inflammation) / Interleukin-6 / Ligation / male / Membrane Proteins / Mice / Mice, Inbred C57BL / 再生 (regeneration) / STAT3 Transcription Factor / シグナル伝達 (signal transduction) / Submandibular Gland
(徳島大学機関リポジトリ): ● Metadata: 111340
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.253
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20224192; ● Search Scopus @ Elsevier (PMID): 20224192; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.253

(徳島大学機関リポジトリ: 111340, DOI: 10.2152/jmi.56.253, PubMed: 20224192) Tetsuya Akamatsu, Azlina Ahmad, Purevjav Javkhlan, Takahiro Hasegawa, Chenjuan Yao and Kazuo Hosoi :
Salivary Gland Development: Its mediation by a subtilisin-like proprotein convertase, PACE4,
The Journal of Medical Investigation : JMI, Vol.56, No.Suppl, 241-246, Tokushima, Dec. 2009.

(要約): The submandibular gland (SMG) develops under the epithelial-mesenchymal interaction. Its process is regulated by various growth/differentiation factors, which are synthesized as inactive precursors and activated via the limited proteolysis at their multi basic amino acid site(s) such as Arg-X-Lys/Arg-Arg. Although many of these processing steps are elucidated to be catalyzed by subtilisin-like proprotein convertases (SPCs), little is known about the role of SPCs in the SMG development. Here, we focused upon the physiological role of PACE4 (SPC4), a member of SPC family, in the SMG development. In the organ culture system of rat embryonic SMG (E15), Dec-RVKR-CMK, a potent inhibitor for SPCs, inhibited the salivary branching and the expression of an exocrine gland type water channel, AQP5. However, other peptidyl-CMKs and inhibitors for trypsin-like serine proteases including leupeptin did not affect the salivary branching and AQP5 expression. Dec-RVKR-CMK also suppressed the expression of PACE4, but not furin, another member of the family. The specific antibody for the catalytic domain of PACE4 suppressed the salivary branching and AQP5 expression similarly. These inhibitory effects of Dec-RVKR-CMK were partially rescued by the addition of recombinant BMP2 whose precursor is a candidate for the physiological substrates of PACE4. Further, the transcriptional silencing of PACE4 by its specific siRNAs caused the suppression of both the salivary branching and AQP5 expression in the present organ culture system. These observations strongly support the idea that PACE4 mediates the SMG development.
(キーワード): Animals / Aquaporin 5 / Bone Morphogenetic Protein 2 / Morphogenesis / Proprotein Convertases / Rats / Salivary Glands / Signal Transduction
(徳島大学機関リポジトリ): ● Metadata: 111337
(出版サイトへのリンク): ● Publication site (DOI): 10.2152/jmi.56.241
(文献検索サイトへのリンク): ● PubMed @ National Institutes of Health, US National Library of Medicine (PMID): 20224189; ● Search Scopus @ Elsevier (PMID): 20224189; ● Search Scopus @ Elsevier (DOI): 10.2152/jmi.56.241

(徳島大学機関リポジトリ: 111337, DOI: 10.2152/jmi.56.241, PubMed: 20224189) Chenjuan Yao, Ahmad Azlina, Javkhlan Purevjav, Takahiro Hasegawa, Tetsuya Akamatsu and Kazuo Hosoi :
Potential down-regulation of parotid gland AQP5 by LPS via cross-coupling of NF-κB/AP1,
The 11th International Symposium on Exocrine Secretion, Tokushima 09 ''Exocrine Secretion Mechanism and Disease'', Tokushima, Jul. 2009. Ahmad Azlina, Nunuk Purwanti, Mileva R. Karabasil, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Kazuo Hosoi :
Is AQP5 down-regulated via autophagic pathway following chorda tympani denervation?,
The International Symposium on Oral Sciences to Improve the Quality of Life, organized by Toshihiko Nagata, Tokushima, Sep. 2008. Mileva R. Karabasil, Takahiro Hasegawa, Ahmad Azlina, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Shigemasa Tomioka and Kazuo Hosoi :
Analyses of rat AQP5 G103D mutant expressed in MDCK-II cells and Xenopus oocytes,
The International Symposium on Oral Sciences to Improve the Quality of Life, organized by Toshihiko Nagata, Tokushima, Sep. 2008. Nunuk Puwanti, Daisuke Tsuji, Mileva Ratko Karabasil, Chenjuan Yao, Takahiro Hasegawa, Tetsuya Akamatsu, Kouji Itou and Kazuo Hosoi :
Activation of IL-6/STAT3/Sca-1 system induces proliferation of duct cells in the duct-ligated mouse submandibular gland,
The 2nd International Symposium on The Future Direction of Oral Sciences in The 21st Century-Oral Sciences for Our Healthy Life-, organized by Toshihiko Nagata, Tokushima, Dec. 2007. Mileva R. Karabasil, Kwartarini Murdiastuti, Takahiro Hasegawa, Nunuk Purwanti, Ahmad Azlina, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
A naturally occurring point mutation in rat AQP5 influencing its membrane trafficking in and water secretion from salivary gland,
The 5th International Conference of Aquaporin, Nara, Jul. 2007. Xuefei Li, Ahmad Azlina, Mileva R. Karabasil, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Effects of autonomic denervation and administration of M3 muscarinic receptor agonist on the AQP5 expression in the rat submandibular gland,
The 5th International Conference of Aquaporin, Nara, Jul. 2007. Chenjuan Yao, Mileva R. Karabasil, Nunuk Purwanti, Ahmad Azlina, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
LPS induces down-regulation of AQP5 and AQP1 via Nf-kB and MAPK pathways in the mouse parotid gland,
The 5th International Conference of Aquaporin, Nara, Jul. 2007. Tetsuya Akamatsu, Nunuk Purwanti, Ahmad Azlina, Mileva R. Karabasil, Xuefei Li, Chenjuan Yao, Norio Kanamori and Kazuo Hosoi :
Prenatal expression of rat salivary AQP5 is dependent on the submandibular gland development mediated by a subtilisin-like proprotein convertase PACE4,
The 5th International Conference of Aquaporin, Nara, Jul. 2007. Nunuk Puwanti, Daisuke Tsuji, Ahmad Azlina, Mileva Ratko Karabasil, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori, Kouji Itou and Kazuo Hosoi :
Alterations of AQP5, cellular markers of duct, and Sca-1 expression in the duct-ligated mouse submandibular gland,
The 5th International Conference of Aquaporin, Nara, Jul. 2007. Mileva R. Karabasil, Takahiro Hasegawa, Nunuk Purwanti, Kwartarini Murdiastuti, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Point mutation of AQP5 in Sprague-Dawley rats and its implication for salivary gland physiology,
The 1st International Symposium and Workshop on The Future Direction of Oral Sciences in The 21st Century, organized by Bando, E., Awaji, Mar. 2007. Xuefei Li, Mileva R. Karabasil, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Effects of autonomic denervation and administration of muscarinic receptor agonist on the expression of AQPs in the rat submandibular gland,
The 1st International Symposium and Workshop on The Future Direction of Oral Sciences in The 21st Century, organized by Bando, E., Awaji, Mar. 2007. Nunuk Purwanti, Daisuke Tsuji, Mileva Ratko Karabasil, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori, Kouji Itou and Kazuo Hosoi :
Changes of cellular markers of duct/acinni and side populations in the duct-ligated mouse submandibular gland,
The 1st International Symposium and Workshop on The Future Direction of Oral Sciences in The 21st Century, organized by Bando, E., Awaji, Mar. 2007. Mileva R. Karabasil, Takahiro Hasegawa, Nunuk Purwanti, Kwartarini Murdiastuti, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Molecular and cellular analyses of mutant AQP5 which occurred naturally in Spraque - Dawley rats,
3rd International Symposium on Salivary Glands in Honor of Niels Stensen, Okazaki, Japan, Oct. 2006. Xuefei Li, Mileva R. Karabasil, Nunuk Purwanti, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Effects of autonomic denervation and administration of SNI-2011 on the expression of AQPs in the rat salivary gland,
The 3rd International Symposium on Salivary Glands in honor of Niels Stensen, Okazaki, Japan, Oct. 2006. Chenjuan Yao, Mileva R. Karabasil, Nunuk Purwanti, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Tissue kallikrein mK13 is a candidate of the processing enzyme for pro-IL-1β in the mouse submandibular gland,
The 3rd International Symposium on Salivary Glands in honor of Niels Stensen, Okazaki, Japan, Oct. 2006. Tetsuya Akamatsu, Nunuk Purwanti, Mileva R. Karabasil, Xuefei Li, Chenjuan Yao, Norio Kanamori and Kazuo Hosoi :
Involvement of a subtilisin-like proprotein convertase, PACE4, in branching morphogenesis and AQP5 expression in the rat embryonic submandibular gland.,
The 3rd International Symposium on Salivary Glands in honor of Niels Stensen, Okazaki, Japan, Oct. 2006. Nunuk Purwanti, Daisuke Tsuji, Mileva Ratko Karabasil, Xuefei Li, Chenjuan Yao, Tetsuya Akamatsu, Norio Kanamori, Kouji Itou and Kazuo Hosoi :
The expression of cellular markers of duct/acini and side population dynamics in the duct-ligated mouse submandibular gland.,
3rd International Symposium on Salivary Glands in Honor of Niels Stensen, Okazaki, Oct. 2006. Chenjuan Yao, Mileva R. Karabasil, Nunuk Purwanti, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Proteolytic processing of the precursor of interleukin-1β by tissue kallikrein mK13 in the submandibular gland of mice.,
International symposium on "Medical and Biological Perspectives in Proteases and Their Inhibitors", Satellite meeting of the 20th IUBMB International Congress of Biochemistry and Molecular Biology & the 11th FAOBMB Congress, Awaji, Japan, Jun. 2006. Tetsuya Akamatsu, Nunuk Purwanti, Mileva R. Karabasil, Xuefei Li, Chenjuan Yao, Norio Kanamori and Kazuo Hosoi :
Inhibition of subtilisin-like proprotein convertase PACE4 reduces the branching morphogenesis and expression of AQP5 in the organ culture system of rat embryonic submandibular gland,
International symposium on "Medical and Biological Perspectives in Proteases and Their Inhibitors", Satellite meeting of the 20th IUBMB International Congress of Biochemistry and Molecular Biology & the 11th FAOBMB Congress, Awaji, Japan, Jun. 2006. 赤松徹也, プルワンティヌヌク, カラバシルミレーバ, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
サチライシン様前駆体蛋白質変換酵素PACE4の阻害はラット胎仔顎下腺の分枝形成とAQP5発現を抑制する,
20th IUBMB International Congress of Biochemistry and Molecular Biology & 11th FAOBMB Congress, 第79回日本生化学会大会 & 第29回日本分子生物学会年会, also 第59回日本細胞生物学会大会, 京都, 2006年6月. Chenjuan Yao, Xuefei Li, Tetsuya Akamatsu, Norio Kanamori and Kazuo Hosoi :
Induction, processing, and secretion of IL-1βin the submandibular gland of mice,
The 35th International Congress of Physiological Sciences (IUPS2005) and Experimental Biology 2005, San Diego, Mar. 2005. Tetsuya Akamatsu, Chenjuan Yao, Xuefei Li, Norio Kanamori and Kazuo Hosoi :
ECM-bound proprotein convertase PACE4 is involved in the branching morphogenesis of rat submandibular gland,
The 35th International Congress of Physiological Sciences (IUPS2005) and Experimental Biology 2005, San Diego, Mar. 2005. 細井和雄, 松浦幸子, 倉淵真悟, 津村恵子, 多田淳, 澤孝賢, 金森憲雄, 赤松徹也 :
肥満細胞キニノーゲンと唾液腺カリクレイン-キニン系,
The 1st meeting for international collaborative research project 1998-2000., 愛知, 1999年1月. Kazuo Hosoi, Shingo Kurabuchi, Yamato Kikkawa, Jun Tada, Tetsuya Akamatsu, Naoki Yamanaka, Takuya Matsumoto, Norio Kanamori and Keiko Tsumura :
Salivary gland tissue kallikrein family and processing of growth factor precursors and proenzymes.,
European Journal of Morphology, Vol.36, No.suppl, 82-85, Cagliari, Italy, Aug. 1998.

(要約): マウスの唾液腺(顎下腺)から得られる主要な組織カリクレインを同定しこれら酵素の有する成長因子前駆体プロセッシング活性について調べた．本研究ではこれら酵素の基質として天然の基質蛋白質(キニノゲン，およびプロレニン)に加えて各種合成基質を用いた．その結果，キニノーゲナーゼ活性の弱い組織カリクレインほど強いプロレニン変換活性を示すことがあきらかになった．

Kenji Mori, 今牧明義, Tetsuya Akamatsu, Akihiko Tsuji, Masami Nagahama and Yoshiko Matsuda :
A novel human PACE4 isoform, PACE4E, is an active protease containing a hydrophobic cluster at the carboxy terminus,
17th International Congress of Biochemistry and Molecular Biology, San Francisco, Aug. 1997. Akihiko Tsuji, Chiemi Hine, Shigeru Yoshida, Miwa Bando, Kenji Mori, Tetsuya Akamatsu and Yoshiko Matsuda :
Gene organization and Alternative Splicing of Human PACE4, Subtilisin-Like Proprotein Convertase,
17th International Congress of Biochemistry and Molecular Biology, FASEB.J., Vol.11, No.9, 1224, San Francisco, Aug. 1997. Hideaki Nagamune, Kazuaki Muramatsu, Masakatsu Higashine, Tetsuya Akamatsu, Yasuhiro Tamai, Yasuo Yonetomi, Akihiko Tsuji, Keisuke Izumi, Takemasa Sakaguchi, Tetsuya Yoshida and Yoshiko Matsuda :
Cyclic AMP-inducible procalcitonin processing in thyroidal parafollicular cells is regulated by the Kexin family protease, PC1.,
International Symposium on Medical Aspects of Protease Inhibitors; In "Medical Aspects of Proteases and Protease Inhibitors": Biomedical and Health Research, Vol.15, 22-33, Amsterdam, May 1997.

(要約): ラット甲状腺においてKexin様プロテアーゼの免疫組織化学的解析を行った．その結果，カルシトニンを作る傍濾胞細胞ではPC1とPC2が強く発現しており，FurinやPC6の発現はわずかで，PACE4は検出できなかった．傍濾胞細胞株TTのウエスタンブロットでもPC1，PC2， Furin，PC6は検出されたが，cAMP刺激による成熟カルシトニン量の上昇と連動して急増する酵素はPC1だけであり，cAMP刺激に応答してカルシトニン量を制御するKexin様プロテアーゼはPC1である事を示した．
(キーワード): Calcitonin / Processing / PC1

赤松徹也, 吉田茂, 大黒成夫, 長宗秀明, 長濱正巳, 辻明彦, 松田佳子 :
ラット脳発生過程における新規PACE4アイソフォーム,PACE4Eの発現,
The 12th International Symposium of Federation of Asian and Oceanian Biochemists and Molecular Biologists, 徳島, 1996年6月. 平田愛佳, 佐藤匠, 増田尚輝, 姚陳娟, 向井理恵, 渡辺崇人, 三戸太郎, 赤松徹也 :
コオロギ食が唾液腺機能に及ぼす効果の検証,
第1回唾液ケア研究会学術集会, 2022年11月. 平田愛佳, 佐藤匠, 田中寛人, 増田尚輝, 姚陳娟, 向井理恵, 長谷川敬展, 吉村弘, 渡辺崇人, 三戸太郎, 赤松徹也 :
食品成分が唾液腺機能に及ぼす影響,
第34回唾液腺談話会, 2022年9月. 植野美彦, 櫻谷英治, 関陽介, 上岡麻衣子, 浅田元子, 赤松徹也, 宮脇克行, 宇都義浩, 田中保 :
一般選抜後期日程における入学辞退率改善の取り組みーー徳島大学B学部の事例からーー,
第17回大学教育カンファレンスin徳島, 2022年1月. 平田愛佳, 佐藤匠, 田中寛人, 小西冴季, 姚陳娟, 向井理恵, 赤松徹也 :
マウス唾液腺AQP5発現に及ぼす高脂肪食および低タンパク質食摂取の影響,
第63回歯科基礎医学会学術大会, 2021年10月. 福田崇子, 楠進太郎, 前田さおり, 長谷川敬展, 姚陳娟, 赤松徹也, 吉村弘 :
周辺環境が主観的感覚および摂食行動に及ぼす影響:筋電図を用いた研究,
日本味と匂学会第55回大会, 2021年9月. 楠進太郎, 福田崇子, 前田さおり, 姚陳娟, 長谷川敬展, 赤松徹也, 吉村弘 :
快・不快環境下における脳活動と摂食行動の関連性,
第55回日本味と匂い学会, 2021年9月. 平田愛佳, 佐藤匠, 田中寛人, 小西冴季, 姚陳娟, 向井理恵, 赤松徹也 :
高脂肪食や低タンパク質食が引き起こすマウス唾液腺AQP5発現・局在変化,
第75回日本栄養・食糧学会大会, 2021年7月. 佐藤匠, 姚陳娟, 長谷川敬展, 吉村弘, 赤松徹也 :
サチライシン様前駆体蛋白質変換酵素PACE4のラット顎下腺発生過程における局在,
第62回歯科基礎医学会学術大会, 2020年9月. 佐藤匠, 姚陳娟, 長谷川敬展, 吉村弘, 赤松徹也 :
ラット唾液腺発生過程におけるサチライシン様前駆体蛋白質変換酵素PACE4の局在,
第93回日本生化学会大会, 2020年9月. Maeda Saori, Hiroshi Yoshimura, Miyaji Yuji, Hiroyuki Kanayama, Takahiro Hasegawa, Chenjuan Yao and Tetsuya Akamatsu :
Increase in theta-band EEG activities under tasting chocolate with unmatched odor stimulation,
第41回日本神経科学大会, Jul. 2018.

(キーワード): 脳波 (electroencephalogram) / シータ波

前田さおり, 吉村弘, 宮地裕司, 金山宏幸, 長谷川敬展, 姚陳娟, 赤松徹也 :
匂いと味の不一致が引き起こすシータ波領域脳活動,
第95回日本生理学大会, 2018年3月. 前田さおり, 宮地裕司, 金山宏幸, 長谷川敬展, 姚陳娟, 赤松徹也, 吉村弘 :
甘味とニオイのミスマッチが甘味認知に与える影響,
第69回日本生理学会中国・四国地方会, 2017年10月. 畑美緒, 赤松徹也, 姚陳娟, 前田さおり, 宮地裕司, 金山宏幸, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおける雌雄差の影響,
第69回日本生理学会中国・四国地方会, 2017年10月. 前田さおり, 吉村弘, 宮地ゆうじ, 長谷川敬展, 姚陳娟, 赤松徹也 :
味と匂いのミスマッチが味覚認知に与える影響:脳波周波数分析を用いた研究,
日本味と匂学会第51回大会, 2017年9月. 吉村弘, 須貝外喜夫, 加藤伸郎, 冨永貴志, 冨永洋子, 長谷川敬展, 姚陳娟, 赤松徹也 :
カフェイン投与により誘発される視覚野オシレーションにおけるnon-NMDA受容体とNMDA受容体の相互交錯的関与,
第40回日本神経科学大会, 2017年7月. Maeda Saori, Miyachi Yuki, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu and Hiroshi Yoshimura :
Influences of olfactory stimulation on taste perception: An electroencephalogram frequency analysis study,
The 94th Annual Meeting of the Physiological Science of Japan, Mar. 2017.

(キーワード): taste perception / olfaction / 脳波 (electroencephalogram)

赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生過程で見られる雌雄差について,
第58回歯科基礎医学会学術大会, 2016年8月. 嶋谷達哉, 嶺岸誠, 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおけるサチライシン様前駆体蛋白質変換酵素PACE4の発現誘導-Part II-,
第58回歯科基礎医学会学術大会, 2016年8月. Hiroshi Yoshimura, 川邊真道, 須貝外喜夫, 加藤伸郎, Takahiro Hasegawa, Chenjuan Yao and Tetsuya Akamatsu :
Influences of oral impairment on neural oscilltion and wave propagation in the neocortex of rats,
第38回日本神経科学大会, Jul. 2015. 赤松徹也 :
唾液腺の構造と機能,
第47回四国歯学会, 2015年6月. 姚陳娟, 赤松徹也, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおける水チャネルAQP5の発現,
第56回歯科基礎医学会, 2014年9月.

(キーワード): 唾液腺 (salivary gland) / 再生 (regeneration) / 水チャネル (water channel) / AQP5

長谷川敬展, 姚陳娟, 赤松徹也, 吉村弘 :
ユビキチンリガーゼによるAQP5のユビキチン化亢進とダウンレギュレーション,
第56回歯科基礎医学会, 2014年9月.

(キーワード): 水チャネル (water channel) / ユビキチン化 / ダウンレギュレーション / AQP5

嶺岸誠, 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおけるサチライシン様前駆体蛋白質変換酵素の発現誘導,
第56回歯科基礎医学会(福岡国際会議場/福岡県), 2014年9月.

(キーワード): 唾液腺 (salivary gland) / 再生 (regeneration) / 前駆体蛋白質変換酵素

赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生におけるサチライシン様前駆体蛋白質変換酵素PACE4の関与,
第55回歯科基礎医学会(2013年9月19日-21日), 2013年9月. 長谷川敬展, 姚陳娟, 赤松徹也, 吉村弘 :
ヒト唾液腺HSG細胞における構成的なAQP5の取り込み,
第55回歯科基礎医学会[2013年9月19日(金)-21日(日)], 2013年9月. 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生におけるサチライシン様前駆体蛋白質変換酵素PACE4の関与,
Journal of Oral Biosciences, Vol.55, No.Suppl., 199, 2013年9月. 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生におけるサチライシン様前駆体蛋白質変換酵素PACE4の関与,
第55回歯科基礎医学会[2013年9月19日(金)-21日(日)], 2013年9月. 吉村弘, 須貝外喜夫, 姚陳娟, 赤松徹也, 長谷川敬展, 加藤伸郎 :
ラット大脳皮質におけるnon-NMDA受容体活動に依存する20Hzオシレーション,
第36回日本神経科学大会[2013年6月20日(木)-23日(日)], 2013年6月. 長谷川敬展, 姚陳娟, 赤松徹也, 吉村弘 :
ヒト唾液腺HSG細胞における構成的なAQP5の取り込み,
Journal of Oral Biosciences, Vol.55, No.Suppl., 199, 2013年. 長谷川敬展, 姚陳娟, 赤松徹也, 細井和雄 :
唾液腺細胞におけるアクアポリン5の翻訳後修飾,
第53回歯科基礎医学会学術大会サテライトシンポジウムSS11, 2011年9月. 長谷川敬展, 姚陳娟, 赤松徹也, 細井和雄 :
唾液腺細胞におけるアクアポリン5の翻訳後修飾, --- 第53回歯科基礎医学会学術大会(2011年9月30日-10月2日)サテライトシンポジウムSS11 ---,
Journal of Oral Biosciences, Vol.53, No.Supplement, 104, 2011年9月. Purevjav Javkhlan, Yuka Hiroshima, Ahmad Azlina, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Toshihiko Nagata and Kazuo Hosoi :
Calprotectin expression in the mouse salivary gland: Induction by lipopolysaccharide,
第52回歯科基礎医学会(2010年9月20-22日), Sep. 2010. 廣島佑香, Purevjav Javkhlan, Ahmad Azlina, 長谷川敬展, 赤松徹也, 細井和雄 :
Porphyromonas gingivalis由来LPSはヒト好中球からのレジスチンの遊離を促進する,
第52回歯科基礎医学会(2010年9月20-22日), 2010年9月. 長谷川敬展, Ahmad Azlina, Purevjav Javkhlan, 廣島佑香, 姚陳娟, 赤松徹也, 細井和雄 :
唾液腺細胞におけるアクアポリン5リン酸化のシグナル伝達機構,
第52回歯科基礎医学会(2010年9月20-22日), 2010年9月. Purevjav Javkhlan, Yuka Hiroshima, Ahmad Azlina, Takahiro Hasegawa, Chenjuan Yao, Tetsuya Akamatsu, Toshihiko Nagata and Kazuo Hosoi :
Calprotectin expression in the mouse salivary gland: Induction by lipopolysaccharide,
Journal of Oral Biosciences, Vol.52, No.supplement, 94, Sep. 2010. 廣島佑香, フルジャフジャフラン, Ahmad Azlina, 長谷川敬展, 赤松徹也, 細井和雄 :
Porphyromonas gingivalis由来LPSはヒト好中球からのレジスチンの遊離を促進する,
Journal of Oral Biosciences, Vol.52, No.supplement, 148, 2010年9月. 長谷川敬展, Ahmad Azlina, フルジャフジャフラン, 廣島佑香, 姚陳娟, 赤松徹也, 細井和雄 :
唾液腺細胞におけるアクアポリン5リン酸化のシグナル伝達機構,
Journal of Oral Biosciences, Vol.52, No.supplement, 164, 2010年9月. 姚陳娟, Ahmad Azlina, フルジャフジャフラン, 長谷川敬展, 赤松徹也, 細井和雄 :
NF-κB/AP-1の複合体を介した耳下腺AQP5のLPSによるdown-regulation,
第51回歯科基礎医学会, 2009年9月. 赤松徹也, Ahmad Azlina, フルジャフジャフラン, 長谷川敬展, 姚陳娟, 細井和雄 :
唾液腺の発生と細胞分化,
第51回歯科基礎医学会, 2009年9月. 長谷川敬展, Ahmad Azlina, フルジャフジャフラン, 姚陳娟, 赤松徹也, 細井和雄 :
アクアポリン5のユビキチン化とその細胞内膜輸送系における役割,
第51回歯科基礎医学会, 2009年9月. フルジャフジャフラン, 廣島佑香, Ahmad Azlina, 長谷川敬展, 姚陳娟, 赤松徹也, 永田俊彦, 細井和雄 :
マウス唾液腺でのリポ多糖によるカルプロテクチン誘導,
第51回歯科基礎医学会, 2009年9月. Ahmad Azlina, フルジャフジャフラン, 長谷川敬展, 姚陳娟, 赤松徹也, 細井和雄 :
ラット顎下腺での副交感神経切除に続くオートファジーを介したAQP5分解,
第51回歯科基礎医学会, 2009年9月. 赤松徹也 :
唾液腺発生におけるサチライシン様前駆体蛋白質変換酵素PACE4の生理機能,
第51回歯科基礎医学会, 2009年9月. カラバシルミレーバ, 長谷川敬展, アズリナアハマド, プルワンティヌヌク, 姚陳娟, 赤松徹也, 細井和雄 :
SDラットで見られるAQP5 G103D変異は正常な水透過性を示すが，膜へのトラフィッキングが低下した,
第50回歯科基礎医学会, 2008年9月. 姚陳娟, アズリナアハマド, プルワンティヌヌク, カラバシルミレーバ, 長谷川敬展, 赤松徹也, 細井和雄 :
唾液腺AQP5のLPSによるdown-regulationの機構,
第50回歯科基礎医学会, 2008年9月. アズリナアハマド, プルワンティヌヌク, カラバシルミレーバ, 姚陳娟, 長谷川敬展, 赤松徹也, 細井和雄 :
副交感神経切除による顎下腺AQP5のダウンレギュレーション,
第50回歯科基礎医学会, 2008年9月. プルワンティヌヌク, アズリナアハマド, カラバシルミレーバ, 姚陳娟, 長谷川敬展, 赤松徹也, 細井和雄 :
導管結紮マウス顎下腺における導管細胞の増殖へのIL-6-STAT3-Sca-1系の関与,
第50回歯科基礎医学会, 2008年9月. 姚陳娟, アズリナアハマド, プルワンティヌヌク, カラバシルミレーバ, 長谷川敬展, 赤松徹也, 細井和雄 :
LPSによる耳下腺AQP5の発現制御とそのシグナル伝達経路,
第59回日本生理学会中国四国地方会, 2007年11月. 赤松徹也, アズリナアハマド, プルワンティヌヌク, カラバシルミレーバ, 長谷川敬展, 姚陳娟, 細井和雄 :
唾液腺腺房細胞の発生・分化・成熟化と水チャネルAQP5発現,
第59回日本生理学会中国四国地方会, 2007年11月. プルワンティヌヌク, 辻大輔, アズリナアハマド, カラバシルミレーバ, 姚陳娟, 長谷川敬展, 赤松徹也, 伊藤孝司, 細井和雄 :
導管結紮によるマウス顎下腺での一過性のIL-6の増加が幹細胞マーカーSca-1の持続的な上昇を引き起す,
第49回歯科基礎医学会学術大会, 2007年8月. カラバシルミレーバ, 長谷川敬展, アズリナアハマド, プルワンティヌヌク, 姚陳娟, 赤松徹也, 細井和雄 :
MDCK II細胞で発現させたラット変異AQP5の機能解析,
第49回歯科基礎医学会, 2007年8月. アズリナアハマド, カラバシルミレーバ, プルワンティヌヌク, 姚陳娟, 長谷川敬展, 赤松徹也, 細井和雄 :
リソゾーム系による顎下腺AQP5蛋白質発現の調節,
第49回歯科基礎医学会, 2007年8月. 姚陳娟, アズリナアハマド, プルワンティヌヌク, カラバシルミレーバ, 長谷川敬展, 赤松徹也, 細井和雄 :
唾液腺AQP5およびAQP1発現のLPSによるdown-regulation,
第49回歯科基礎医学会, 2007年8月. 赤松徹也, アズリナアハマド, プルワンティヌヌク, カラバシルミレーバ, 長谷川敬展, 姚陳娟, 細井和雄 :
唾液腺腺房細胞の分化とAQP5発現,
第49回歯科基礎医学会, 2007年8月. カラバシルミレーバ, 長谷川敬展, プルワンティヌヌク, 李雪飛, 姚陳娟, 赤松徹也, 金森憲雄, 細井和雄 :
in vitroシステムで発現したラット変異AQP5の機能解析と異常唾液分泌への影響,
第48回歯科基礎医学会, 2006年9月. 李雪飛, プルワンティヌヌク, カラバシルミレーバ, 姚陳娟, 赤松徹也, 金森憲雄, 細井和雄 :
ラット唾液腺AQP5，AQP1，及びリソゾーム酵素系に対する自律神経切除とSNI2011投与の影響,
第48回歯科基礎医学会, 2006年9月. 姚陳娟, カラバシルミレーバ, プルワンティヌヌク, 李雪飛, 赤松徹也, 金森憲雄, 細井和雄 :
LPSによる唾液腺AQP5およびAQP1の発現制御とそのシグナル伝達経路,
第48回歯科基礎医学会, 2006年9月. 赤松徹也, プルワンティヌヌク, カラバシルミレーバ, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
顎下腺の分枝形成とAQP5発現は前駆体蛋白質変換酵素PACE4により調節される,
第48回歯科基礎医学会, 2006年9月. プルワンティヌヌク, 辻大輔, カラバシルミレーバ, 李雪飛, 姚陳娟, 赤松徹也, 金森憲雄, 伊藤孝司, 細井和雄 :
マウス顎下腺における腺房細胞/導管細胞マーカーの発現，およびサイドポピュレーション細胞群の挙動に及ぼす主導管結紮の影響,
第48回歯科基礎医学会学術大会, 2006年9月. 赤松徹也, プルワンティヌヌク, カラバシルミレーバ, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
唾液腺発生におけるsubtilisin-like proprotein convertaseの役割,
第28回分子生物学会年会, ワークショップW3G「プロテアーゼによる細胞分化・機能制御, 植物から動物まで/Proteases as biomodulators in bacteria, plant and animal」, 2005年12月. 李雪飛, 姚陳娟, 赤松徹也, 金森憲雄, 細井和雄 :
交感神経切除，副交感神経切除，およびSNI-2011投与のラット顎下腺におけるAQP5，AQP1発現におよぼす影響,
第78回日本生化学会大会, 2005年10月. 姚陳娟, Nunuk Purwanti, Chisato Kosugi-Tanaka, 赤松徹也, 金森憲雄, 細井和雄 :
カリクレインmK13はマウス唾液腺におけるpro-IL-1βプロセシング酵素の候補である,
第78回日本生化学会大会, 2005年10月. 赤松徹也, Nunuk Purwanti, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
ECM結合型前駆体蛋白質変換酵素PACE4による顎下腺発生過程の調節,
第78回日本生化学会大会, 2005年10月. 李雪飛, 姚陳娟, 赤松徹也, 金森憲雄, 細井和雄 :
ラット顎下腺AQP5並びにAQP1に対する交感神経切除・副交感神経切除及びSNI-2011投与の影響,
第47回歯科基礎医学会, 2005年9月. 姚陳娟, 李雪飛, 赤松徹也, 金森憲雄, 細井和雄 :
マウス唾液腺カリクレインmK13はpro-IL-1βプロセシング酵素の候補である,
第47回歯科基礎医学会, 2005年9月. 赤松徹也, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
顎下腺発生における前駆体蛋白質変換酵素PACE4の関与,
第47回歯科基礎医学会, 2005年9月. 細井和雄, 李雪飛, 姚陳娟, 赤松徹也, 金森憲雄, ミレーバカラバシル, ヌヌクプルワンティ :
外分泌腺における水チャネル，アクアポリンの発現と機能調節,
第47回歯科基礎医学会サテライトシンポジウム「唾液分泌機構研究の最近の進歩」, 2005年9月. 赤松徹也, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
ECM結合型前駆体蛋白質変換酵素PACE4はラット顎下腺の分枝形成における調節因子候補である,
第77回日本生化学会大会, 2004年10月. 姚陳娟, 李雪飛, 赤松徹也, 金森憲雄, 細井和雄 :
マウス顎下腺におけるカリクレインmK13によるpro-IL1βの活性化,
第46回歯科基礎医学会, 2004年9月. 赤松徹也, 李雪飛, 姚陳娟, 金森憲雄, 細井和雄 :
顎下腺の分枝形成と機能発現,
第46回歯科基礎医学会, 2004年9月. 赤松徹也 :
ラット顎下腺発生過程での水チャネルAQPファミリーの発現と局在,
第28回四国歯学会/第9回高田充歯科基礎医学奨励賞受賞講演, 2004年6月. Kawartarini Murdiastuti, Nahid Parvin, 姚陳娟, 李雪飛, 赤松徹也, 金森憲雄, 細井和雄 :
水チャネル，アクアポリンの外分泌腺における発現と機能調節,
第45回日本生化学会中国・四国支部例会, 2004年5月. 赤松徹也, 李雪飛, 姚陳娟, Kawartarini Murdiastuti, 小杉知里, 金森憲雄, 細井和雄 :
ラット顎下腺の発生・分化・成熟と機能発現,
第45回日本生化学会中国・四国支部例会, 2004年5月. 姚陳娟, 李雪飛, 赤松徹也, 金森憲雄, 細井和雄 :
実験的炎症による唾液腺サイトカインの誘導,
第45回歯科基礎医学会, 2003年9月. 金森憲雄, 赤松徹也, Chisato Kosugi, Kawartarini Murdiastuti, Most. Nahid Parvin, 姚陳娟, Osamu Miki, Xuefei Li, 細井和雄 :
ジャンピング:てんかん家系スナネズミに対する行動指標(プレリミナリーレポート),
第26回日本神経科学大会, 2003年7月. 赤松徹也, Most. Nahid Parvin, 小杉-田中知里, 姚陳娟, 金森憲雄, 細井和雄 :
ラット顎下腺発生過程でのAQPsの発現と局在,
第75回日本生化学会大会, 2002年10月. Kawartarini Murdiastuti, 三木修, 姚陳娟, Most. Nahid Parvin, 小杉-田中知里, 赤松徹也, 金森憲雄, 細井和雄 :
Sprague-Dawleyラット顎下腺におけるAQP5の相違する遺伝子発現と局在,
第44回歯科基礎医学会, 2002年10月. 赤松徹也, Most. Nahid Parvin, 小杉-田中知里, 姚陳娟, 金森憲雄, 細井和雄 :
ラット顎下腺発生過程でのAQPsの発現と局在,
第44回歯科基礎医学会, 2002年10月. 金森憲雄, 赤松徹也, 小杉-田中知里, Parvin Nahid Most., 姚陳娟, 細井和雄 :
病態モデルスナネズミ近交化の試み(中間報告),
第25回日本神経科学大会, 2002年7月. Most. Parvin Nahid, 小杉-田中知里, 赤松徹也, 倉淵真悟, 金森憲雄, 細井和雄 :
AQP5のラット十二指腸での発現，局在，およびVIPの効果,
第79回日本生理学会学術大会, 2002年3月. 津村恵子, Most. Parvin Nahid, Kawartarini Murdiastuti, 赤松徹也, 金森憲雄, 稲永清敏, 細井和雄 :
水代謝異常多飲多渇マウス(STR/N)の唾液腺および腎におけるアクアポリン(AQP)の発現,
第43回歯科基礎医学会学術大会, 2001年9月. 赤松徹也, Most Parvin Nahid, Kawartarini Murdiastuti, 金森憲雄, 細井和雄 :
ラット唾液線発生過程でのAQPsの発現,
第43回歯科基礎医学会学術大会, 2001年9月. Most Parvin Nahid, Kawartarini Murdiastuti, 赤松徹也, 金森憲雄, 細井和雄 :
十二指腸ブルナー腺におけるAQP5発現とVIPの効果,
第43回歯科基礎医学会学術大会, 2001年9月. 赤松徹也, 津村恵子, 多田淳, Most. Nahid Parvin, 金森憲雄, 細井和雄 :
口腔組織発生におけるプロセシング酵素PACE4の役割,
日本生化学会第73回大会, 2000年10月. Wei Wei, Most. Nahid Parvin, 津村恵子, 松浦幸子, 赤松徹也, 多田淳, 金森憲雄, 細井和雄 :
ラット唾液腺における急性期蛋白質の誘導,
第42回歯科基礎医学会学術大会, 2000年9月. 多田淳, 津村恵子, 赤松徹也, Most. Nahid Parvin, 金森憲雄, 細井和雄 :
唾液腺培養細胞でのAQP5のトラフィッキング機構,
第42回歯科基礎医学会学術大会, 2000年9月. Most. Nahid Parvin, Wei Wei, 津村恵子, 多田淳, 赤松徹也, 金森憲雄, 細井和雄 :
ラット唾液腺と消化管におけるアクアポリン-5水チャネル発現と局在,
第42回歯科基礎医学会学術大会, 2000年9月. 赤松徹也, 松田佳子, 多田淳, 津村恵子, Most. Nahid Parvin, 金森憲雄, 細井和雄 :
歯牙形成における前駆体蛋白質活性化酵素SPCsの役割,
日本生化学会第72回大会, 1999年10月. 松浦幸子, 鈴木和夫, 赤松徹也, 津村恵子, 多田淳, 細井和雄 :
口腔粘膜・肥満細胞でのキニノーゲンの発現,
第41回歯科基礎医学会学術大会, 1999年9月. 赤松徹也, 津村恵子, 金森憲雄, 多田淳, Most. Nahid Parvin, 細井和雄 :
前駆体蛋白質活性化酵素SPCのラット唾液腺発生過程での発現,
第41回歯科基礎医学会学術大会, 1999年9月. 金森憲雄, 里村一人, 赤松徹也, 細井和雄 :
スナネズミ総頚動脈結紮時の脳血流動態,
第22回日本神経科学大会, 1999年7月. 赤松徹也, 松田佳子, 辻明彦, 津村恵子, 金森憲雄, 細井和雄 :
サチライシン様前駆体蛋白質変換酵素PACE4は歯の形成過程における骨形成蛋白質のプロセシングプロテアーゼの候補である,
第6回日本生化学会春期シンポジウム, 1999年5月. 細井和雄, 津村恵子, 多田淳, 赤松徹也, 金森憲雄, 松浦幸子 :
浸潤唾液腺肥満細胞におけるキニノーゲンの発現,
第40回歯科基礎医学会学術大会, 1998年10月. 丹羽保晴, 李志遠, 岡真紀子, 永田典子, 坂本貞一, 大中正治, 赤松徹也, 中屋豊 :
エンドセリン-1(1-31)を産生する細胞の同定,
日本生化学会第71回大会, 1998年10月. 金森憲雄, 赤松徹也, 細井和雄 :
新たに開発した血管染色法と, それによって判定したスナネズミ大脳動脈輪欠損出現率の系統差,
第21回日本神経科学・第41回日本神経化学合同大会, 1998年9月. 赤松徹也, 吉田茂, 長宗秀明, 辻明彦, 松田佳子 :
ラット脳発生過程でのプロセシング酵素PACE4の発現,
日本生化学会第70回大会, 1997年9月. 赤松徹也, 吉田茂, 大黒成夫, 長宗秀明, 長濱正巳, 辻明彦, 松田佳子 :
プロセシング酵素PACE4Eのラット脳発生過程での発現,
日本生化学会第69回大会・日本分子生物学会第19回年会合同年会, 1996年8月. 赤松徹也, 吉田茂, 水野勝弘, 長田香弥, 米本佳余子, 森健二, 長宗秀明, 辻明彦, Eric Grouzmann, 松田佳子 :
Kexin様プロテアーゼPC7の中枢神経系における細胞特異的発現,
日本生化学会第68回大会, 1995年9月. 辻明彦, 長宗秀明, 赤松徹也, 日根智恵美, 村松和明, 森健二, 玉井靖啓, 米本佳余子, 松田佳子 :
新Kexin様プロテアーゼの構造と組織分布,
日本先天代謝異常学会第36回大会, 1994年11月. 赤松徹也, 今村恵美, 長宗秀明, 辻明彦, 松田佳子 :
新しいkexinファミリープロテアーゼPC7の細胞特異的発現-in situ hybridizationによる解析,
日本細胞生物学会第47回大会, 1994年9月. 玉井靖啓, 村松和明, 赤松徹也, 日根智恵美, 森健二, 長宗秀明, 辻明彦, 泉啓介, 松田佳子 :
新ペプチドホルモンプロセッシングプロテアーゼPACE4Cの細胞特異的発現,
日本細胞生物学会第47回大会, 1994年9月. 長宗秀明, 辻明彦, 村松和明, 赤松徹也, 東根一也, 日根智恵美, 泉啓介, 坂口剛正, 吉田哲也, 野地澄晴, 松田佳子 :
甲状腺傍濾胞細胞におけるサチラーゼファミリープロテアーゼの発現,
日本生化学会第66回大会, 1993年10月. 赤松徹也, 辻明彦, 長宗秀明, 松田佳子 :
ラット肝α-マクログロブリン-プロテアーゼ複合体の性質,
日本生化学会第65回大会, 1992年10月. 辻明彦, 赤松徹也, 尾田領一, 長宗秀明, 松田佳子 :
ラット肝α-マクログロブリン-プロテアーゼ複合体の性質,
日本生化学会中四国支部第55回例会, 1992年4月.

研究会・報告書

姚陳娟, 長谷川敬展, 赤松徹也, 吉村弘 :
マウス耳下腺における水チャネルAQP5のイソプロテレノールによるdouwn-regulationの機構,
Journal of Oral Biosciences, Vol.54, No.Suppl., 153, 姚陳娟, 佐藤匠, 長谷川敬展, 赤松徹也, 吉村弘 :
イソプロパノール反復投与によるマウス唾液腺機能に及ぼす影響,
第34回唾液腺談話会, 2022年9月.

(キーワード): イソプロパノール / 水チャネル (water channel)

嶋谷達哉, 嶺岸誠, 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生モデルにおけるサチライシン様前駆体蛋白質変換酵素PACE4の発現誘導-Part II-,
Journal of Oral Biosciences, Vol.Suppl., 415, 2016年8月. 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生過程で見られる雌雄差について,
Journal of Oral Biosciences, Vol.Suppl., 451, 2016年8月. 赤松徹也 :
基礎系教育講演, --- 唾液腺の構造と機能 ---,
Journal of Oral Health and Biosciences, Vol.28, No.2, 97, 2016年2月. 赤松徹也, 姚陳娟, 長谷川敬展, 吉村弘 :
唾液腺再生への subtilisin-like proprotein convertase PACE4の関与,
自然科学研究機構生理学研究所研究会「唾液腺形態形成研究会~機能解析から器官再生へ~」, 2014年8月.

(キーワード): 唾液腺 (salivary gland) / 再生 (regeneration) / 前駆体蛋白質変換酵素

姚陳娟, 長谷川敬展, 赤松徹也, 吉村弘 :
マウス耳下腺における水チャネルAQP5のイソプロテレノールによるdouwn-regulationの機構,
第54回歯科基礎医学会, 2012年9月. 吉村弘, 長谷川敬展, 姚陳娟, 赤松徹也 :
細胞内cAMP上昇が大脳皮質味覚野から口腔体性感覚野への信号伝播速度に与える影響,
第54回歯科基礎医学会, 2012年9月. 姚陳娟, 長谷川敬展, 赤松徹也, 吉村弘 :
マウス耳下腺における水チャネルAQP5のイソプロテレノールによるdouwn-regulationの機構,
Journal of Oral Biosciences, Vol.54, No.Suppl., 153, 2012年9月. 吉村弘, 長谷川敬展, 姚陳娟, 赤松徹也 :
細胞内cAMP上昇が大脳皮質味覚野から口腔体性感覚野への信号伝播速度に与える影響,
Journal of Oral Biosciences, Vol.54, No.Suppl., 131, 2012年9月. 赤松徹也, 細井和雄 :
顎下腺の発生・分化・成熟と機能発現,
生理学研究所研究会:唾液分泌機構解明に向けての戦略的展開, 2005年2月. 赤松徹也, 細井和雄 :
ラット顎下腺の発生・分化・成熟と機能発現,
生理学研究所研究会, 2004年2月. 細井和雄, 金森憲雄, 赤松徹也, 姚陳娟 :
外分泌腺における水チャネル，特にアクアポリン5の発現と機能調節の分子機構,
平成18年度-平成20年度文部省科学研究費補助金(基盤研究(B))研究成果報告書, 徳島, 2009年3月. 細井和雄, 金森憲雄, 赤松徹也, 小杉知里 :
唾液腺における炎症性サイトカインの誘導と口腔の防御システム:再生医療への試み,
平成16年度-平成17年度文部省科学研究費補助金(萌芽研究)研究成果報告書, 徳島, 2006年3月. 細井和雄, 中條信義, 羽地達次, 赤松徹也 :
肥満細胞由来キニノーゲンによる炎症とその抑制 -抗アレルギー新薬の開発-,
平成13年度-平成15年度文部省科学研究費補助金(基盤研究(B)(2))研究成果報告書, 徳島, 2004年3月. 細井和雄, 金森憲雄, 赤松徹也, 多田淳, 津村恵子 :
水チャネル蛋白質，アクアポリンの唾液腺細胞における発現と機能調節の分子機構,
平成13年度-平成14年度文部省科学研究費補助金(基盤研究(C)(2))研究成果報告書, 徳島, 2003年3月. 細井和雄, 金森憲雄, 松浦幸子, 赤松徹也, 澤孝賢 :
水チャネル蛋白質，アクアポリンの唾液腺細胞における発現と機能調節の分子機構,
平成11年度-平成12年度文部省科学研究費補助金(基盤研究(C)(2))研究成果報告書, 徳島, 2001年3月.

特許: 研究者総覧に該当データはありませんでした。
作品: 研究者総覧に該当データはありませんでした。
補助金・競争的資金: 唾液腺Ca2+応答の低侵襲的長期間イメージング技術の確立と機能・再生研究 (研究課題/領域番号: 16K11484 )
唾液腺再生の分子機構の解明～口腔乾燥症等の再生医療への応用を目指して～ (研究課題/領域番号: 26462814 )
口腔乾燥症の克服に向けた唾液腺腺房細胞の分化・成熟と機能発現の分子機構の解明 (研究課題/領域番号: 23592737 )
病態時における外分泌腺型水チャネル、AQP5の発現と機能調節の分子機構 (研究課題/領域番号: 23592736 )
唾液腺腺房細胞の分化誘導、及び機能発現の分子メカニズムの解明 (研究課題/領域番号: 18791369 )
外分泌腺における水チャネル、特にアクアポリン5の発現と機能調節の分子機構 (研究課題/領域番号: 18390493 )
水チャネル,アクアポリンの唾液腺発生過程における発現・局在及び機能制御機構の解明 (研究課題/領域番号: 16791126 )
唾液腺における炎症性サイトカインの誘導と口腔の防御システム:再生医療への試み (研究課題/領域番号: 16659513 )
網羅的cDNA塩基配列決定を基盤とする膵β細胞特異的に発現する遺伝子の解析 (研究課題/領域番号: 15590943 )
水チャネル蛋白質,アクアポリンの外分泌腺細胞における発現と機能調節の分子機構 (研究課題/領域番号: 13671940 )
肥満細胞由来キニノーゲンによる炎症とその抑制―抗アレルギー新薬の開発― (研究課題/領域番号: 13557154 )
歯・唾液腺発生過程でのプロセシング酵素PACE4の発現制御機構及び生理機能の解明 (研究課題/領域番号: 12771107 )
水チャネル蛋白質、アクアポリンの唾液腺細胞における発現と機能調節の分子機構 (研究課題/領域番号: 11671844 )
口腔組織(歯,舌,唾液腺)発生におけるサチライシン様プロ蛋白質変換酵素の役割 (研究課題/領域番号: 10771013 )
組織カリクレインファミリーの新しい生理機能と成長因子前駆体のプロセッシング (研究課題/領域番号: 08672129 )
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2024年4月19日更新

専門分野・研究分野: 生化学 (Biochemistry)
細胞生理学 (Cell Physiology)
分子細胞生物学 (Molecular Cell Biology)
発生生物学 (Developmental Biology)
所属学会・所属協会: 日本生化学会
日本細胞生物学会
歯科基礎医学会
日本生理学会
日本分子生物学会
四国歯学会
委員歴・役員歴: 歯科基礎医学会 (評議員 [2009年9月〜2016年3月])
四国歯学会 (評議員 [2007年4月〜2018年3月])
受賞: 1994年1月, 康楽賞 (財団法人三木康楽会)
2003年12月, 高田充歯科基礎医学奨励賞 (歯学科)
2009年9月, 第21回歯科基礎医学会賞 (歯科基礎医学会)
2010年7月, 徳島大学歯学部若手研究奨励賞 (歯学科)
2012年3月, 徳島大学歯学部ベストメンター賞 (歯学部)
2012年7月, 歯学部優秀教育賞 (歯学部)
2016年3月, H27年度ベストメンター賞 (歯学部)
活動: 歯学部総合研究室運営委員会委員 (1997年6月〜1999年6月)
歯学部総合研究室運営委員会委員 (2001年6月〜2003年6月)
歯学部総合研究室運営委員会委員 (2003年6月〜2005年6月)

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Ｊグローバル

Jグローバル最終確認日: 2024/4/20 01:14
氏名（漢字）: 赤松徹也
氏名（フリガナ）: アカマツテツヤ
氏名（英字）: Akamatsu Tetsuya
所属機関: 徳島大学准教授

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researchmap最終確認日: 2024/4/14 01:15
氏名（漢字）: 赤松徹也
氏名（フリガナ）: アカマツテツヤ
氏名（英字）: Akamatsu Tetsuya
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登録日時: 2007/3/15 00:00
更新日時: 2024/4/13 06:31
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eメール: akamatsu_t@tokushima-u.ac.jp
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所属ID: 0344000000
所属: 徳島大学
部署: 大学院社会産業理工学研究部食料科学分野
職名: 准教授
学位: 博士(工学)
学位授与機関: 徳島大学
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研究者番号: 80294700

所属（現在）: 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授

所属（過去の研究課題 情報に基づく）*注記: 2024/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授
2017/4/1 – 2018/4/1 : 徳島大学, 大学院社会産業理工学研究部(生物資源産業学域), 准教授
2016/4/1 : 徳島大学, 大学院生物資源産業学研究部, 准教授
2015/4/1 : 徳島大学, 大学院医歯薬学研究部, 准教授
2011/4/1 – 2014/4/1 : 徳島大学, ヘルスバイオサイエンス研究部, 准教授
2011/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授
2007/4/1 – 2008/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教
2006/4/1 – 2007/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助手
2005/4/1 – 2006/4/1 : 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助手
2004/4/1 : 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助手
1997/4/1 – 2003/4/1 : 徳島大学, 歯学部, 助手

審査区分/研究分野

研究代表者

医学 / 歯学 / 機能系基礎歯科学
生物系 / 医歯薬学 / 歯学 / 機能系基礎歯科学
小区分59040:栄養学および健康科学関連

研究代表者以外

生物系 / 医歯薬学 / 歯学 / 機能系基礎歯科学
医学 / 歯学 / 機能系基礎歯科学
生物系 / 医歯薬学 / 内科系臨床医学 / 代謝学

キーワード

研究代表者

サチライシン様プロ蛋白質変換酵素 / プロセシング / 口腔組織 / 発生 / 歯牙形成 / 骨形成蛋白質 / 唾液腺 / 分枝形成 / in situハイブリダイゼーション / bHLH型転写因子 / 顎下腺 / 器官培養 / 水チャネル / AQP5 / プロセシング酵素 / PACE4 / RNAi / 腺房細胞 / HSG細胞 / サチライシン様前駆体蛋白質変換酵素 / 導管細胞 / サチライシン様前駆体蛋白質酵素 / 発生・分化・再生 / 再生 / 雌雄差

研究代表者以外

唾液腺 / TLR4 / 炎症性サイトカイン / IL-1β / カリクレインmK13 / 幹細胞特異抗原 / アクアポリン / カテプシン / 副交感神経 / 塩酸セビメリン / MDCK細胞 / トラフィッキング / GFP-AQP5キメラ分子 / アクアポリン5 / LPS / 遺伝子変異 / 鼓索神経切除 / 組織カリクレインファミリー / プロレニン変換酵素 / 真性組織カリクレイン / マウス / プロセシング酵素 / 組織カリクレイン / プロレニン / プロセッシング / 成長因子 / Tissue kallikrein family / Proreninn converting enzyme / True tissue kallikrein / Submandibular gland / Mouse / Processing enzyme / 消化管 / 十二指腸 / ブルナー腺 / 細胞骨格 / 細胞内カルシウムイオン / aquaporins / trafficking / salivary gland / gastrointestinal tract / duodenum / Brunner's gland / キニノーゲン / 急性期蛋白質 / 外分泌腺 / Kininogen / Acute phase protein / Inflammation cytokine / exocrine gland / Salivary gland / VIP / SDラット / 遺伝的変動 / Sprague-Dawley rat / genetic variation / 膵β細胞 / セリンプロテアーゼ / エンドソーム / pancreatic islet β cells / serine protease / endosome / in vivo / 蛍光 / カルシウム / 遺伝子 / ウイルス / カルシウム応答 / イメージング / 蛍光タンパク質 / ウイルスベクター / アセチルコリン / in vivoイメージング / 光ファイバー / 薬理学 / 生理学 / バイオテクノロジー

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赤松 徹也

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赤松徹也